LOCUS       DACECJ010000001        96314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.001, whole genome shotgun sequence.
ACCESSION   DACECJ010000001 DACECJ010000000
VERSION     DACECJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 96314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.001"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            83..601
                     /locus_tag="IWO09_000001"
     CDS             83..601
                     /locus_tag="IWO09_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0262629.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            729..1412
                     /locus_tag="IWO09_000002"
     CDS             729..1412
                     /locus_tag="IWO09_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0262630.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1721..1794
                     /locus_tag="IWO09_000003"
     tRNA            1721..1794
                     /locus_tag="IWO09_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1755..1757,aa:Arg,seq:tct)
     gene            1953..2312
                     /locus_tag="IWO09_000004"
     CDS             1953..2312
                     /locus_tag="IWO09_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0262631.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2435..4066
                     /locus_tag="IWO09_000005"
     CDS             2435..4066
                     /locus_tag="IWO09_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0262632.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4128..4769)
                     /locus_tag="IWO09_000006"
     CDS             complement(4128..4769)
                     /locus_tag="IWO09_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0262633.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5051..5359
                     /gene="rplU"
                     /locus_tag="IWO09_000007"
     CDS             5051..5359
                     /gene="rplU"
                     /locus_tag="IWO09_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0262634.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5386..5730
                     /locus_tag="IWO09_000008"
     CDS             5386..5730
                     /locus_tag="IWO09_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0262635.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5743..6036
                     /gene="rpmA"
                     /locus_tag="IWO09_000009"
     CDS             5743..6036
                     /gene="rpmA"
                     /locus_tag="IWO09_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0262636.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6443..9277
                     /locus_tag="IWO09_000010"
     CDS             6443..9277
                     /locus_tag="IWO09_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0262637.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9468..9959
                     /locus_tag="IWO09_000011"
     CDS             9468..9959
                     /locus_tag="IWO09_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0262638.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10062..11051
                     /gene="manX"
                     /locus_tag="IWO09_000012"
     CDS             10062..11051
                     /gene="manX"
                     /locus_tag="IWO09_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0262639.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11084..11887
                     /locus_tag="IWO09_000013"
     CDS             11084..11887
                     /locus_tag="IWO09_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0262640.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11908..12822
                     /locus_tag="IWO09_000014"
     CDS             11908..12822
                     /locus_tag="IWO09_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0262641.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12905..13279
                     /locus_tag="IWO09_000015"
     CDS             12905..13279
                     /locus_tag="IWO09_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0262642.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13418..13663
                     /locus_tag="IWO09_000016"
     CDS             13418..13663
                     /locus_tag="IWO09_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0262643.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13835..14899
                     /locus_tag="IWO09_000017"
     CDS             13835..14899
                     /locus_tag="IWO09_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0262644.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15145..15570
                     /locus_tag="IWO09_000018"
     CDS             15145..15570
                     /locus_tag="IWO09_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0262645.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15563..16012
                     /gene="nusB"
                     /locus_tag="IWO09_000019"
     CDS             15563..16012
                     /gene="nusB"
                     /locus_tag="IWO09_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0262646.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16109..16963
                     /locus_tag="IWO09_000020"
     CDS             16109..16963
                     /locus_tag="IWO09_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0262647.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16964..18301
                     /locus_tag="IWO09_000021"
     CDS             16964..18301
                     /locus_tag="IWO09_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0262648.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18306..18530
                     /locus_tag="IWO09_000022"
     CDS             18306..18530
                     /locus_tag="IWO09_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0262649.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18532..19422
                     /locus_tag="IWO09_000023"
     CDS             18532..19422
                     /locus_tag="IWO09_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0262650.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19431..20249
                     /locus_tag="IWO09_000024"
     CDS             19431..20249
                     /locus_tag="IWO09_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0262651.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20356..20805
                     /locus_tag="IWO09_000025"
     CDS             20356..20805
                     /locus_tag="IWO09_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0262652.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20823..22505
                     /gene="recN"
                     /locus_tag="IWO09_000026"
     CDS             20823..22505
                     /gene="recN"
                     /locus_tag="IWO09_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0262653.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22560..22676)
                     /locus_tag="IWO09_000027"
     CDS             complement(22560..22676)
                     /locus_tag="IWO09_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0262654.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22749..23705)
                     /locus_tag="IWO09_000028"
     CDS             complement(22749..23705)
                     /locus_tag="IWO09_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0262655.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23876..24244
                     /locus_tag="IWO09_000029"
     CDS             23876..24244
                     /locus_tag="IWO09_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0262656.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24414..24845
                     /gene="mraZ"
                     /locus_tag="IWO09_000030"
     CDS             24414..24845
                     /gene="mraZ"
                     /locus_tag="IWO09_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0262657.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24866..25825
                     /gene="rsmH"
                     /locus_tag="IWO09_000031"
     CDS             24866..25825
                     /gene="rsmH"
                     /locus_tag="IWO09_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0262658.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25830..26231
                     /gene="ftsL"
                     /locus_tag="IWO09_000032"
     CDS             25830..26231
                     /gene="ftsL"
                     /locus_tag="IWO09_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0262659.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26232..28424
                     /locus_tag="IWO09_000033"
     CDS             26232..28424
                     /locus_tag="IWO09_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0262660.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28450..29412
                     /locus_tag="IWO09_000034"
     CDS             28450..29412
                     /locus_tag="IWO09_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0262661.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29426..30787
                     /locus_tag="IWO09_000035"
     CDS             29426..30787
                     /locus_tag="IWO09_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0262662.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30813..31901
                     /gene="murG"
                     /locus_tag="IWO09_000036"
     CDS             30813..31901
                     /gene="murG"
                     /locus_tag="IWO09_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0262663.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31916..33136
                     /locus_tag="IWO09_000037"
     CDS             31916..33136
                     /locus_tag="IWO09_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0262664.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33292..34617
                     /gene="ftsA"
                     /locus_tag="IWO09_000038"
     CDS             33292..34617
                     /gene="ftsA"
                     /locus_tag="IWO09_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0262665.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34640..35881
                     /gene="ftsZ"
                     /locus_tag="IWO09_000039"
     CDS             34640..35881
                     /gene="ftsZ"
                     /locus_tag="IWO09_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0262666.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35898..36575
                     /locus_tag="IWO09_000040"
     CDS             35898..36575
                     /locus_tag="IWO09_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0262667.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36589..37188
                     /locus_tag="IWO09_000041"
     CDS             36589..37188
                     /locus_tag="IWO09_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0262668.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37203..37490
                     /locus_tag="IWO09_000042"
     CDS             37203..37490
                     /locus_tag="IWO09_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0262669.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37539..38321
                     /locus_tag="IWO09_000043"
     CDS             37539..38321
                     /locus_tag="IWO09_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0262670.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38342..39049
                     /locus_tag="IWO09_000044"
     CDS             38342..39049
                     /locus_tag="IWO09_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0262671.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39311..42109
                     /gene="ileS"
                     /locus_tag="IWO09_000045"
     CDS             39311..42109
                     /gene="ileS"
                     /locus_tag="IWO09_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0262672.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42158..43684)
                     /locus_tag="IWO09_000046"
     CDS             complement(42158..43684)
                     /locus_tag="IWO09_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0262673.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43699..44346)
                     /locus_tag="IWO09_000047"
     CDS             complement(43699..44346)
                     /locus_tag="IWO09_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0262674.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44530..44859)
                     /locus_tag="IWO09_000048"
     CDS             complement(44530..44859)
                     /locus_tag="IWO09_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0262675.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45036..45764
                     /gene="sfsA"
                     /locus_tag="IWO09_000049"
     CDS             45036..45764
                     /gene="sfsA"
                     /locus_tag="IWO09_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0262676.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45780..46793
                     /locus_tag="IWO09_000050"
     CDS             45780..46793
                     /locus_tag="IWO09_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0262677.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46793..48070
                     /locus_tag="IWO09_000051"
     CDS             46793..48070
                     /locus_tag="IWO09_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0262678.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48133..50835
                     /locus_tag="IWO09_000052"
     CDS             48133..50835
                     /locus_tag="IWO09_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0262679.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50987..51304
                     /locus_tag="IWO09_000053"
     CDS             50987..51304
                     /locus_tag="IWO09_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0262680.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51334..51654
                     /locus_tag="IWO09_000054"
     CDS             51334..51654
                     /locus_tag="IWO09_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0262681.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51762..53222
                     /locus_tag="IWO09_000055"
     CDS             51762..53222
                     /locus_tag="IWO09_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0262682.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53290..53511
                     /locus_tag="IWO09_000056"
     CDS             53290..53511
                     /locus_tag="IWO09_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262683.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53542..53724
                     /locus_tag="IWO09_000057"
     CDS             53542..53724
                     /locus_tag="IWO09_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0262684.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53724..54137
                     /locus_tag="IWO09_000058"
     CDS             53724..54137
                     /locus_tag="IWO09_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0262685.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54260..55441
                     /locus_tag="IWO09_000059"
     CDS             54260..55441
                     /locus_tag="IWO09_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0262686.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55830..55903
                     /locus_tag="IWO09_000060"
     tRNA            55830..55903
                     /locus_tag="IWO09_000060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55864..55866,aa:Arg,seq:cct)
     gene            complement(55940..56140)
                     /locus_tag="IWO09_000061"
     CDS             complement(55940..56140)
                     /locus_tag="IWO09_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0262687.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56445..57677)
                     /locus_tag="IWO09_000062"
     CDS             complement(56445..57677)
                     /locus_tag="IWO09_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0262688.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57931..58500
                     /locus_tag="IWO09_000063"
     CDS             57931..58500
                     /locus_tag="IWO09_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0262689.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58678..59118)
                     /locus_tag="IWO09_000064"
     CDS             complement(58678..59118)
                     /locus_tag="IWO09_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0262690.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59276..60040
                     /gene="map"
                     /locus_tag="IWO09_000065"
     CDS             59276..60040
                     /gene="map"
                     /locus_tag="IWO09_000065"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0262691.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60072..60995
                     /locus_tag="IWO09_000066"
     CDS             60072..60995
                     /locus_tag="IWO09_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0262692.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61071..62210
                     /locus_tag="IWO09_000067"
     CDS             61071..62210
                     /locus_tag="IWO09_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0262693.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62203..63003
                     /locus_tag="IWO09_000068"
     CDS             62203..63003
                     /locus_tag="IWO09_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0262694.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            63003..63830
                     /locus_tag="IWO09_000069"
     CDS             63003..63830
                     /locus_tag="IWO09_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0262695.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63808..64542
                     /locus_tag="IWO09_000070"
     CDS             63808..64542
                     /locus_tag="IWO09_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0262696.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64642..65508
                     /gene="rfbA"
                     /locus_tag="IWO09_000071"
     CDS             64642..65508
                     /gene="rfbA"
                     /locus_tag="IWO09_000071"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0262697.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65522..66094
                     /gene="rfbC"
                     /locus_tag="IWO09_000072"
     CDS             65522..66094
                     /gene="rfbC"
                     /locus_tag="IWO09_000072"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0262698.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66116..67144
                     /gene="rfbB"
                     /locus_tag="IWO09_000073"
     CDS             66116..67144
                     /gene="rfbB"
                     /locus_tag="IWO09_000073"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0262699.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67242..68093
                     /gene="rfbD"
                     /locus_tag="IWO09_000074"
     CDS             67242..68093
                     /gene="rfbD"
                     /locus_tag="IWO09_000074"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0262700.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68128..70161
                     /locus_tag="IWO09_000075"
     CDS             68128..70161
                     /locus_tag="IWO09_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262701.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70205..71485
                     /locus_tag="IWO09_000076"
     CDS             70205..71485
                     /locus_tag="IWO09_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0262702.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71695..72501
                     /locus_tag="IWO09_000077"
     CDS             71695..72501
                     /locus_tag="IWO09_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0262703.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72513..73733
                     /locus_tag="IWO09_000078"
     CDS             72513..73733
                     /locus_tag="IWO09_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0262704.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73723..75309
                     /locus_tag="IWO09_000079"
     CDS             73723..75309
                     /locus_tag="IWO09_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0262705.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75348..77486
                     /locus_tag="IWO09_000080"
     CDS             75348..77486
                     /locus_tag="IWO09_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0262706.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77854..78837)
                     /locus_tag="IWO09_000081"
     CDS             complement(77854..78837)
                     /locus_tag="IWO09_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0262707.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79215..80606
                     /locus_tag="IWO09_000082"
     CDS             79215..80606
                     /locus_tag="IWO09_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0262708.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80622..81662
                     /locus_tag="IWO09_000083"
     CDS             80622..81662
                     /locus_tag="IWO09_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0262709.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81681..82766
                     /locus_tag="IWO09_000084"
     CDS             81681..82766
                     /locus_tag="IWO09_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0262710.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            82799..83761
                     /locus_tag="IWO09_000085"
     CDS             82799..83761
                     /locus_tag="IWO09_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0262711.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            83754..85181
                     /locus_tag="IWO09_000086"
     CDS             83754..85181
                     /locus_tag="IWO09_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0262712.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            85183..86253
                     /locus_tag="IWO09_000087"
     CDS             85183..86253
                     /locus_tag="IWO09_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262713.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            86240..87661
                     /locus_tag="IWO09_000088"
     CDS             86240..87661
                     /locus_tag="IWO09_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0262714.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            87674..88681
                     /locus_tag="IWO09_000089"
     CDS             87674..88681
                     /locus_tag="IWO09_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0262715.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            88693..89697
                     /gene="neuB"
                     /locus_tag="IWO09_000090"
     CDS             88693..89697
                     /gene="neuB"
                     /locus_tag="IWO09_000090"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0262716.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            89694..90842
                     /gene="neuC"
                     /locus_tag="IWO09_000091"
     CDS             89694..90842
                     /gene="neuC"
                     /locus_tag="IWO09_000091"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0262717.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            90815..91492
                     /locus_tag="IWO09_000092"
     CDS             90815..91492
                     /locus_tag="IWO09_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0262718.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            91482..92624
                     /locus_tag="IWO09_000093"
     CDS             91482..92624
                     /locus_tag="IWO09_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0262719.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            92637..93614
                     /locus_tag="IWO09_000094"
     CDS             92637..93614
                     /locus_tag="IWO09_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0262720.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            93614..94522
                     /locus_tag="IWO09_000095"
     CDS             93614..94522
                     /locus_tag="IWO09_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0262721.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            94523..95275
                     /locus_tag="IWO09_000096"
     CDS             94523..95275
                     /locus_tag="IWO09_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0262722.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            95280..96227
                     /locus_tag="IWO09_000097"
     CDS             95280..96227
                     /locus_tag="IWO09_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0262723.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        31958 a        15607 c        19945 g        28804 t
ORIGIN      
        1 catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa gaaggattga
       61 ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta taagttctat
      121 aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc gaatttttgg
      181 tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac aaggaaatct
      241 gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta ttacatatta
      301 tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa acaaccacga
      361 agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag taaaaaagag
      421 aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc tttcaaaaac
      481 ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa caggaatcac
      541 agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga aatttttgta
      601 agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa catgaaacac
      661 gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt gtgaagagga
      721 ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa ctattggatt
      781 ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa gaagaagctc
      841 atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa tttttacttg
      901 gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt tcatcaagtg
      961 tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt ttcgatgtca
     1021 tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa gtgactttgc
     1081 ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac tttattttta
     1141 tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt ttgttaagaa
     1201 aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg ctcccagatg
     1261 aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt cttcgtgttg
     1321 tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt gtgatcttaa
     1381 tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc tgtagataat
     1441 cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt gtaagtcaat
     1501 taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta gctcctgttt
     1561 tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga ttgatttagt
     1621 agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat tgacagaagc
     1681 aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag ctcagctgga
     1741 tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt gacgctaaaa
     1801 aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca atattgtttg
     1861 ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata aggagcaaaa
     1921 aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga attttagtga
     1981 ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga actgattctg
     2041 acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa tctgttcaaa
     2101 cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta ctttatggag
     2161 cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct attttttttg
     2221 ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa ggtgggctag
     2281 ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa tttatctaac
     2341 aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta atgaatggta
     2401 ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg tttcctcact
     2461 aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt tggctgggat
     2521 cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa gcggagctga
     2581 tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg gtttatcagg
     2641 cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga tgtctattgc
     2701 ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc tatttttact
     2761 tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac ccgtaattac
     2821 ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata atttatttgg
     2881 tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa cgataggatt
     2941 tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga tgtttatcta
     3001 tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt tggcaacagc
     3061 agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac aaacattgat
     3121 cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac aagtggcagt
     3181 tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg gtgcttcggc
     3241 tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc aaggaattgg
     3301 aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg cccgtacaag
     3361 cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg cagttgtctt
     3421 actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc cagcacttgc
     3481 aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca aggagctatt
     3541 tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt gtacagttat
     3601 ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat tttttgttgt
     3661 caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa tggggctgcc
     3721 agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac tgttttttat
     3781 gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg gtgtcatttt
     3841 ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg atgaatttca
     3901 agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc cagaagtaga
     3961 aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa cagttgctca
     4021 tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca atgaaaaaga
     4081 acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta atattgctgt
     4141 aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga gcgattgtag
     4201 aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga ccattcgtag
     4261 gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc atgatttttg
     4321 gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag tgtcgcaatt
     4381 aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc aaatacttcg
     4441 gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg tttattgatc
     4501 cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac ctcttctcgt
     4561 gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc gctaaaagga
     4621 acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag tttaggatga
     4681 agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct taaagaagct
     4741 tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt tattataaga
     4801 ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag aaaagcagaa
     4861 gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta acggttgctg
     4921 ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc tcagaacaag
     4981 taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc agaaggaggt
     5041 gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag ttgaagtggg
     5101 acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg tttttgacga
     5161 agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg caggtgcaac
     5221 tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt tcaaatacaa
     5281 acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa aagtggttat
     5341 cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat caaaggaaca
     5401 tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca tgctgatgct
     5461 ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat cagcactgtc
     5521 aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga agaagaaggc
     5581 ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa tattgctgag
     5641 attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa ctcagaatat
     5701 atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt atctatgaat
     5761 ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg tgactcagaa
     5821 tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg atcaattctt
     5881 taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg tggagacgac
     5941 actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg taacaaaaaa
     6001 caagtgtctg tttatccagt agctcaagaa gcttaattga atcttatgat taaagaggtt
     6061 gtgacaagac ttttgtcaca gccttttttg tttataaaaa taaaagttgt agtggaggct
     6121 acgaagttta ctttgtcttt ttacacttaa tatttttttg ccaatttgga atcaggatgt
     6181 tttgataact atcgttttca attgatggaa gctatattgt ttgctatcag tgaaataatt
     6241 atatggtaaa cagtgttctt tttcttgttt ccattataaa aatcgtatcg ggatttttcg
     6301 ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa aatcaggtaa
     6361 atagttgacg aaacagaaat aaatacatag tattatgttt tgaagcctaa gtacaatagt
     6421 ctatagaaaa ggagcgatac cattggcaaa acgcattgac cgaatttata tgtatgtcaa
     6481 agaaaaaacc gctcatttaa caaaggcaga atatgatcaa ggagtaacaa cacaggaaat
     6541 tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa gatctgaatc agctagtgcg
     6601 cgaaggaaaa ctgataaaga cagacggacg accagtacgt tacatatatc aaacactcgt
     6661 tacgcacgta aagccagtta caaaacatgt agtcagttac aaagaaacgc cggtctctga
     6721 agaaaaaaat acactccaaa tagatactaa ggatatcttt gcaaaaatca ttggagctaa
     6781 cggcagcatg aaaaattcag tggaacaggc aaaagctgcg attttgtatc cgccaagagg
     6841 attaaattgt ttgattacag gacctaccgg atcaggaaag acttattttg cacacgcgat
     6901 gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa acggaactga ttgttttcaa
     6961 ttgtgctgat tatgccaata accctgagct tttgatgagt catttatttg ggtacgcaaa
     7021 aggtgctttt acaggcgcag atgaagaaaa aatggggatt atcgatcaag cggatggcgg
     7081 tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa tgatctttta
     7141 ctttatggat catggaactt acagtcgtct tggggaaaca acgaaatccc atgaagcaaa
     7201 tgtacggatc gtaggtgcga ccacagaaga cccaggttct tctctattag aaacttttgt
     7261 tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg caaatgagaa
     7321 agtcgattta gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc gaaagatttc
     7381 tttgacagaa gatgtagcaa aagcattgat cggcagtgtg acctatggaa atataggaca
     7441 attgaagtca aatatccagt tagtatgtgc acgaggattt ctaaatcata tgcaatcacc
     7501 cgaaatctcg atcacaatcg atgatttgac agaagggatt cgtagcggat tgattcaatt
     7561 agcaagcaac cgcagtgcaa tggcagagct ttctaaaata ttggagccaa agataactgt
     7621 agcaccaaat gatacgatca tgaaaatcca atcggactcc tatgagttac cttataatct
     7681 atatgatatc atcggagata aagcagcttt actgaagtcg gatggtttgg atcaagaagc
     7741 aatcaatcat ttcatttcta cagatatcaa catccatctg aagtctttct ataaagacca
     7801 tggtttttct tttaatgcag acagcaaatt agcagaattt gtagatccga aaatcataga
     7861 agtgaccaat cagatttatg taatggtcaa aagatcttta ccatatgagt ttcagcaaaa
     7921 ctttatttat gctatgagct tgcatatcag ttcttttttg aaaaaaatca acttaggaga
     7981 agaacggcat acgaatgata acatccgtga gatggctttt gattttccag atgaatatgc
     8041 tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt caagtggaga ttccagaaag
     8101 tgaagactat tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag caagcggcag
     8161 aatcggtgta gtcgttgcag ctcacggaaa cagcacagca agcagtatgg tccaagttgt
     8221 ccaacagttg ttagatgcag agaacgtacg agcagtagac atgcctttgg atatggatcc
     8281 gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag gaagcggggt
     8341 catactctta gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc aaagagacac
     8401 tggaattcca gtacgtactg ttgatatggt aacaacttct ctagttttgg aaactgtacg
     8461 aaaggtctca gtattaggga cagatctaga tatgttgcat gaatcattga aaaattttcg
     8521 aggttacgca gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta ttcataagaa
     8581 aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa tcaaagaatt
     8641 gatcgaacga gcggtaaaca agcgtcaaga aacagaatta gaagttctag ctttgtcgat
     8701 tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa tatcagatca tagctactac
     8761 tgggattact gatttgaata tccaagcccc atttattcct ttagaacgtt ttattgatca
     8821 aaatatcgaa gtcattttgg atcagttgct gatggaatca gaattggagg aaactgaatt
     8881 catctcccta gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct ctgataactt
     8941 catctttatt aatggtagta agttaatcga tccaatgtgg caattttcta cacaaatctc
     9001 tcagacgacg ggaatcggcg atgaagaata tggattcaaa atcaacctag tgatgcacac
     9061 agcaggaatg atcgaacgta ttattcggaa tgaaccattg actgtggaag aaaacgaatt
     9121 gacaaataca acgaatgatc ctctttatag ccaattggca gcctcagttg ttctgttgga
     9181 agatcagata aaagtaaaag tgccgataga agaaatgtat tatctgctac gtctagtcca
     9241 taatcagtta gataaaaaag aatacactgt gccatgaata cacaaaaacg atacactttt
     9301 atagtgtatc gtttttgtgt attggttaag aatcctttaa attcaattag tgtatcgtaa
     9361 aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg ttcacacaga
     9421 aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg gatattcgat
     9481 tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg tcgaagcaaa
     9541 caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa ttgagaaagt
     9601 ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc gtgcagaaaa
     9661 tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt ttattcacca
     9721 atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg ttgaatatcg
     9781 gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca gtaaatcaac
     9841 aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag attcgaaaag
     9901 tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa aaggtgtagc
     9961 aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa ttattaatat
    10021 ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg attatcctag
    10081 caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc tttggcgaac
    10141 aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac ttgagagcca
    10201 aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc ttagttgatt
    10261 tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac aaagataaat
    10321 gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca tcacgctttt
    10381 caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa gacggtgtaa
    10441 aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt gaagacgcac
    10501 aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc aaatatgtat
    10561 tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg acaaaagcag
    10621 tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat ctgcgtaaga
    10681 gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct attagcaaaa
    10741 tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt ttatttgaaa
    10801 atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa ttaaatgttg
    10861 ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct atgggacaag
    10921 aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat gtacgtaaag
    10981 tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa gctgaattag
    11041 caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta ttatttcaat
    11101 aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg atgaatttca
    11161 atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta acctcgaagc
    11221 tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa atatcggtgc
    11281 cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt tagttttagg
    11341 tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt tggctgttgc
    11401 agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc atttgatgga
    11461 tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca ttgctgtatg
    11521 tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc cagcacaaac
    11581 tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg ctatcggtgg
    11641 gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta caaaagaagt
    11701 atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa cattgattgc
    11761 tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta aaatgggtgg
    11821 ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca ttttgaatga
    11881 ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt atcaaaaaaa
    11941 gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa ctacgaacgt
    12001 atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt atacaaaaca
    12061 aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac acacccatac
    12121 attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc gaatggcgcg
    12181 cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc tttagctggt
    12241 gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact aggtgcttca
    12301 ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg gaacattatt
    12361 cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc taaaattact
    12421 gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat tttagggatg
    12481 ttcgtattag ctgcccttgt acaaagatgg gtatcaatca aattcttacc agttgtatca
    12541 actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc aggtggtgaa
    12601 ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc tgaaaaggta
    12661 acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt aggattaaca
    12721 ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat ccttggttta
    12781 ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa aatatagctc
    12841 gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata tctggaggga
    12901 gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta cttcgtttac
    12961 gggtctgaca gattacgggc aaatcatgat aggtgataat ggttttgagt tttaccatgc
    13021 gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg tactagcatc
    13081 agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa aaaatggtac
    13141 atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag aatatgtaga
    13201 tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg gtattcgtag
    13261 tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt ctttattttt
    13321 ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct ttaaatccca
    13381 tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat ggacattgat
    13441 tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa attcgatggg
    13501 ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag cgattttcgg
    13561 cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag gcgcagtgat
    13621 tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta caaaatagta
    13681 aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat ttaaaataaa
    13741 aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt tgctataatg
    13801 gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag aaaaattgcg
    13861 caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt acaatcttcg
    13921 ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg aaaaagcatt
    13981 ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag ggtttgaaat
    14041 tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca aagaagaact
    14101 gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg ttttagaaga
    14161 aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca gagaagtgaa
    14221 agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata tggcatatga
    14281 tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa atcaactaga
    14341 cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg ttgctagcgg
    14401 attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac aaggtgactt
    14461 gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga cacgtacatt
    14521 cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct tagatgctca
    14581 attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag atgcagttgc
    14641 tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta caggacatgg
    14701 gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa agcaatttgt
    14761 accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg gtggagtacg
    14821 tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc gttcaccaaa
    14881 agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat cagtgttcac
    14941 agactttgtt atagaaaatc actcgattta atgatacact atagaagaac ttttaagctg
    15001 taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc tgatcggcgg
    15061 cagttttctt accacgtgat cactatgccg catcatagag aattcggatg tatctcgtgc
    15121 ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt aaacgcaaac
    15181 caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat cgggattgca
    15241 gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa tgtcactgaa
    15301 ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga agatggcttg
    15361 aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt agcaatggaa
    15421 atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt atctgaagtc
    15481 aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat cgaagaactt
    15541 tttgaagacg aagaggaaga gaatgaataa agaattatca agacacgaga ttcgggaaat
    15601 ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag aagatgcgat
    15661 tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat cagagtttgt
    15721 tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg aattggataa
    15781 agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa aaatggattt
    15841 ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac cagcggctgt
    15901 tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt cacgtaaatt
    15961 cgtcaacggt gttttatcca atgtattgaa agaattagaa gctggggcgt aagtcctagc
    16021 ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg gtagaatgaa
    16081 aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa aagaattagc
    16141 tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata atggtatcca
    16201 tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct atgtaagaaa
    16261 taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt atcctgagac
    16321 gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc ctagttttca
    16381 tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga tcctgttagc
    16441 tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca gactccttgc
    16501 gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt ttgaagctta
    16561 ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata tcgtcggtaa
    16621 accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc attctaaaac
    16681 cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa taggacgtgg
    16741 acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg tagggatgaa
    16801 tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg agcctattgc
    16861 aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga tgctgatgta
    16921 tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac aagaatattt
    16981 aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc cttatttaga
    17041 acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg cccatcaata
    17101 tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag gagcattcca
    17161 aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc gtatttcatt
    17221 atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg atggaatagg
    17281 tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag gcctttttca
    17341 agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta ccagtccaag
    17401 cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa ttgtccaatt
    17461 ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg tcaggaatat
    17521 ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg ggggtggttc
    17581 gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt atcaagctaa
    17641 tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg atttagtagc
    17701 agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg ttttgtcaga
    17761 agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg tacgccagat
    17821 ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc ggcagcctga
    17881 aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat tatatcaagc
    17941 tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc accaattgga
    18001 acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt atctagaaaa
    18061 acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt ttgctcaagc
    18121 tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct acagctatac
    18181 gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg atttgctgac
    18241 gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca aaggagaata
    18301 aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa attgtcatgc
    18361 agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa cgaggaatgg
    18421 aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg acgaaaatga
    18481 tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga gatgagatta
    18541 actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc ttttttagat
    18601 gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa agcaggcggg
    18661 aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga gcctattgat
    18721 gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc cttgatccac
    18781 gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac caatcataaa
    18841 gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg agcattccag
    18901 ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca gcaattagct
    18961 gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga gggcgaatcg
    19021 aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg gcatttgatt
    19081 cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat cttattgcta
    19141 cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga tctactcgat
    19201 gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg tatggaaaaa
    19261 aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga gattgagctg
    19321 ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt tgatggcggg
    19381 ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga atggaaaaag
    19441 aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa caagccaaac
    19501 gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat aaaccgggag
    19561 aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct tatgtttctc
    19621 gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt gaaggaaaaa
    19681 cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg caaaatggcg
    19741 caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa ataagacaag
    19801 atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc gaagacttta
    19861 ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta aaactgatgc
    19921 tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta atcaaacccc
    19981 aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac cctcagaccc
    20041 atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat gatgtgctgg
    20101 aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg gctcacttga
    20161 ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa gtggtttcaa
    20221 atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag tgagaagtat
    20281 gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa gaatatgcta
    20341 aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg attgttgagt
    20401 gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa aggaatatct
    20461 gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa ggtaccggct
    20521 tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt aaatactaaa
    20581 ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa atttgttatt
    20641 ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa acgttacaag
    20701 aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac acgaactgaa
    20761 gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga ggagcgaatg
    20821 tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg cggctttctt
    20881 ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc attattatcg
    20941 atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt caaggagcag
    21001 aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc tctgaactga
    21061 tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg gatatgtcct
    21121 taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct aatctgagaa
    21181 gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa ctgctccagc
    21241 ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa cagaagaaaa
    21301 agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta cgaaaagtgc
    21361 agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag caagaagaaa
    21421 tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa agagaaaaac
    21481 ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca ctgacgaata
    21541 atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa gggatcgccc
    21601 atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac tatttgctgc
    21661 aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat gagagtcgtt
    21721 tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa tacggcgaat
    21781 ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct tcttctgatt
    21841 tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag cttctacttc
    21901 agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa ttggaaaaat
    21961 ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag gtacgtttct
    22021 taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa ttttacatta
    22081 cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt ggcgaactgt
    22141 ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg acaagtatta
    22201 tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc gcagataaaa
    22261 tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct caagttgctg
    22321 cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt acgcaaactt
    22381 ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg ctggctggaa
    22441 gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc gcaaaaagga
    22501 cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat attttatttt
    22561 tacatgacac tcattacagc agtcaataca acagatccaa tcgttaaaat cagcattgtc
    22621 caaatgacaa attttgttac tttagcaaaa gtacttgttt gttttttttc acgcattgtt
    22681 ttcaacctcg tcattaatta gttgattcaa gtgtacaatc ttttttccat ttccacaaga
    22741 gggctttttt ataaaaactt cccttttttt aaataatagg tagttacgat acaaataaat
    22801 aatgttaata gaactagcca tataaaggca tcatcccttt cagcaatagg taacttaatg
    22861 ttcattccat agatactgcc gacgattgtc ggtatagtta aaataatcgt taaagaagtt
    22921 aaaattttca taatattatt cagattattc gatacgattg ctgaaaaagt ctgattcatt
    22981 tgttctacta gttttaattg aatgcgagaa gtagtcattg cctgctctgt ttccaccaat
    23041 acatcacgta aatgattttt caaagcatga ttttcttgga aaagtttatt ttgcgctagt
    23101 tctttcaatg catctaaatt agcagaagtc gcttctctga aataaacgag gcttttttgg
    23161 atatccatga tttgataaag ttgtttgttt tcagtggata cttgaagttt ggcttccaaa
    23221 gtatcgcatt gagattgaag cgtagctaaa taactgttgt aagagagtgt gacctgccaa
    23281 atgaattgaa agaagatatt gagattaggc gaaatatcac tttttggaaa ggtctggcta
    23341 aagactttct ttaaaaacag tggttcatga ttcgtaattg tgataagctt ctttttgggc
    23401 gtcaaaataa tagagagcgg gtatgtatcc acttgaaaat aaccgctagg acttgtttta
    23461 gcaaaaggat attgtaacaa taataacgct ggctttgtca actttgattg attgaattct
    23521 tctgtacggg aattttctgc atcgtccaat atggaagtga tgtaatctga cggaatttca
    23581 aattgatgtg tcagctgatg aatctcttct tgattaggac gttctacaca ataccagatc
    23641 gtttggtcag tttcctttcc ggtattacgc agtttctcat tatctaatgt aaaatatttg
    23701 atcatgattt tcctcctgtt gcctgtttct tttattataa cggataggga attggaatac
    23761 atggaatgtg tttgtaagat atagaatata actgcataaa tttaaataaa atgaaacagt
    23821 tgtgtaaagt atatacagta tacttgaagc aacgataaaa aaggagttta cgttcatggg
    23881 cacattcaca ccacttatct tgttttggta tatttacccg gtaatcgttt tgtttggttg
    23941 ccaatttctt gtaaaattat tttcgttaaa tcgtcgtttt aaaataaaag cccctgattt
    24001 ggcgattcct tttttgtggg gcggtatgca tgcattgtct agaaatacag ggacaatttc
    24061 tatcttaccg tttttcttta tttctgtttt gctaatggga atttgtattg ctgttttcca
    24121 agcatattat tatgaagaaa ttatttatcc gcgttatttc aagatgactt ggcgtttggt
    24181 ttttctgttg acaatcgtct tgtacgctgt cttgatcatt gtgaacatac tttcttacct
    24241 atgaaaaaaa tagaaaagct gtaaaaagtc ttttaaaagg ctttttgcag cttgattttt
    24301 aaaaaaatgt ttatgaataa aaaaagttgt tcccggattt cccagaaaag gtggtagaaa
    24361 gtgggggatt gtggtagact aattttagtt agtggaggaa tggggggctt tcaatgttga
    24421 tgggtgaatt ccaacataat attgatgcaa aaggacgttt aatcgtgccc tcaaaacttc
    24481 gtgaagaact tggcgaaaag tttgttctga cacgcggatt ggatggctgt cttttcggct
    24541 atccgatgag cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg ttggctaaaa
    24601 aagacgcacg tacttttgtg cgattcttct attcggctgc cactgaatgc gaacttgata
    24661 aacaaggacg aatcaatata cccagcacgt taagaaatta tgcagctttg acaaaaggtt
    24721 gcgtagtcat cggtgtatct aaccgaattg aaatttggga tgagacacga tggcaagatt
    24781 tttctgcagc agcagaagag aactttgacg aaattgctga aagtatgatt gatttcggac
    24841 tataagaata ggaagaggct gactaatgac tgaaacattt caacactata cagtcatgtt
    24901 gaaagaaacg gtagatggat tgaacatcaa accggatggc gtttatgtag actgtacact
    24961 cggcggtgcc ggacatagtg aatatttatt aagccaacta agttctaaag ggcatctgta
    25021 tgcgtttgac caagatcaaa aagcaatcga tcacgccaag ctgcgtttag cttcttatgt
    25081 cgaacaaggt atggtcacat ttatcaaagc gaacttcaga gaattagaag aacgcttgag
    25141 tgagcatgta gataaggtag acggcatttt gtatgatcta ggggtttcct ctcctcagct
    25201 agatgaagca gaacgtggat ttagttatca tcaagatgca ccgttggata tgcgcatgga
    25261 tcagtcagct cctttatccg cttatcacgt aattaatgaa tattcctacc atgaattagt
    25321 aaagattttc ttccgctatg gcgaggagaa attttccaaa caaattgctc gtgaaatcga
    25381 acgagtacga aaaacaaaac caatccaaac aactggtgag cttgtcgaaa tcattaaaca
    25441 agtcatccca gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa tttttcaagc
    25501 tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag gcctctttag aacaggcaat
    25561 ccggttattg aaagtcaatg gaagaatcag cgtaatcaca ttccattcat tggaagacag
    25621 aattgtcaaa agtatgttta aagaatatag tacggtacaa gatctaccac cgggacttcc
    25681 tgtcatacct gacgaattcc aaccagaact gaaagtcatt actcgtaaac caatccttcc
    25741 ttcagaggaa gagctaacag aaaacaatcg atcacgcagt gcaaagttac gtattgcaga
    25801 aaaaataaga atgaaagagg agtagaatga tggcagaatt aaagaaaatg caagattacg
    25861 aatatgattt acctgaattg gtggatcagc cggaacaaca aatgcctgat tcttcttcga
    25921 atcaagaaat cattcttcca caatcaccgg caaaacgatt aaagcagatt tctgctttgg
    25981 aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt ttaacagtta
    26041 tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa acagaaatca
    26101 atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt tctcgtactg
    26161 aacggattaa acaaatcgca gaagaaaaag gattgtctat taatgacgac aatttaagga
    26221 aagtgaagta aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag aaaaatctaa
    26281 atccgatgaa caatcgcaaa aaagtaggta tcattttatt tgctacgagt attggattgt
    26341 tctttttatt tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt gctggtacct
    26401 cattagctga aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa gcaaaaagag
    26461 gaacgattta tgatcgtaac ggtgtggcgc tcgcagaaga tgcctcctct tattcaatta
    26521 aagcgatact ctcaaaaaca tatacctctg gggataaaaa actgtatgtt gaagaaaaga
    26581 attttgataa aatagcagaa atcctgcata agaatctttc tatagacaaa aaagatgcac
    26641 tgaatatttt agaggacggc gcaaaaaaag agctgtatca agttgaattt ggtagctatg
    26701 ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa aaagaaggtg
    26761 tcgctggtct ttattttgtc gaccatcagg ctcggatgta tccaaatggc gtcttttctt
    26821 ctcattttat tggatacgca gttccagaca aagatgagaa tggcttagtt ggtaaactag
    26881 gtttggagtc tgcttacaat gatatcttga gcggtaaaga tggtaagatt atttatcaaa
    26941 aagataattt tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa gctgtcgacg
    27001 gacaagatat ctacacgact ttagacagca gactacaaag ttatttagaa acactgatgg
    27061 atcaagtaaa tgaagaatat cagccagaag aattgacagc agtcctgatg aaagcaaaaa
    27121 ccggagaaat cttggcaatg ggacaacgac ctactttcaa tcctgaaaca atggaaggat
    27181 tgactggaaa agatgctatc tggcgtaatt tcttggtaca agatagttat gaaccaggat
    27241 caaccatgaa agttttcact actgcagctg ctatcgaaga aggagaattc aacgagaatg
    27301 aaaccttcca atctggaaaa atccaagtag aagatgctac gatcaatgac catgactttg
    27361 gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac gtcgggatgg
    27421 tcatactgga acagcgatta ggcggacgat ggtataacta tttacaaaaa ctgggatttg
    27481 gacaaagtac tcattctgga ttagatgatg aagtaaacgg tgctttacct acattaaata
    27541 ttgtcgaccg agcaatgagt gcttatggac aagcggtagg tgtgacgaat ttccaaatga
    27601 tgaaagcttt cacttctatt gcgaataacg gtacaatgat tcaacctcga tatatcagca
    27661 aagtggtgga tccccaaact ggagaagagc gaacaactca aacagaagtt ctagggcaac
    27721 ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt gtcgaaagcg
    27781 aaaactacgg tagtgcttat ggtgtatata gtgtacctgg atacaatgta tcggcaaaaa
    27841 acggaacagc ccaaatcgct tctgatacag ggggatatca aacaggagat acagcctacc
    27901 tgtattcgat tgtggagatg gttccttctg aagatccaga ctatgtcttg tatctaacaa
    27961 tgaaacaccc taagacatat gatcgaatgg cattggctaa aatcgcaaat ccattgatga
    28021 aacgggcaat ggattttaaa gaaactgaag aagattcaga tacagaaaca aaaacagaaa
    28081 aagtatccgt agccgattat cgtaatcttg aagcggacgt tgcagcagct gatgctcaaa
    28141 aaagtggact gcaacctgta gtgatcggta atggtaaaaa agtacaaaaa caatctactg
    28201 ccaacggtga tcagttgatc tctggagaaa aactgatcct atatactggt ggtgataagc
    28261 tgatgcctga cgtaacagga tggtcaaaag cagatatcat gaagctgggg aaaatcctcg
    28321 gtattgaagt tagttttgat ggagatggtt attgtgtcaa acaagaactt gctccatatg
    28381 aaaaaatcac tgaagacaaa cttagtttta ctttagaaga ataagaagag aaataggaga
    28441 gaaaagagca tggaatggac acaagcattg attcccattg tcagcagttg tgcaatgaca
    28501 attgccgcaa tgccgctatt tattggttat tttcaaatga aaaagcaagg tcaggcgatt
    28561 cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa ctcctacaat gggcggctta
    28621 gtgtttctga tcggctctat tttgacagga atttgggttg gagcttggca aaaacagcta
    28681 actcctactc ttttcatctt gctttttgta ttagcgttat acggagtaat cggctttctt
    28741 gatgatttta taaaaatatt caagaaaaga aacatgggac tgaactcgaa gcagaaattg
    28801 attggacaga ttctaggggg aatcatcttt tatcttgttt accgttctga aggttatcca
    28861 ggtgtattga atttcttcgg actagatcta cctttaggaa tcatttatgg tattttcgct
    28921 ctcttctggc ttgttggatt ttcaaatgca gtaaatctga cagatggcat cgatggatta
    28981 gttgctggct taggaagtat ctcttttgca gcttacggtc taattgcttg gcatcagcat
    29041 cagtatgatg tattaatcat ttgcttaagt gttttaggag gtttactggg tttcttcgtc
    29101 tacaacagaa aacctgccaa gatttttatg ggagatgtcg gttcattagc gttgggcgga
    29161 cttttagcgg caatttctat aatgctaaac caagaatgga cattgctttt gattggtttg
    29221 atctatgtta tggaaacagc aagtgtgatg cttcaagtta cttcctttaa gcttacagga
    29281 aaacgaattt ttaaaatgtc tccaattcat catcactttg aaatgtgcgg atggtcggag
    29341 tggaagattg atacagtttt ttggttgatc agcattgtga cttcgttgat cacattatgg
    29401 atcgtttggt aggagatgac gggaaatgaa aaaaatatca acatatgaaa ataagaaagt
    29461 gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc aaattattac atgaattggg
    29521 cgcacttgtc accgttaatg atggaaaacc ttttgacgaa aatccggaag cacaagagct
    29581 gctttcttta gggataaaag taataacagg tagtcatcca atcgaattgt tggatgaaga
    29641 attttcattg atggtcaaaa atccagggat tccctacagt catcctttag tagcaaaagc
    29701 ccaagaaatg ggtattccgg taattacgga agttgagcta gcttacgaag tagcggaatg
    29761 cccaattatt gggattacag gaacgaacgg aaaaacaacg acaacaacga tgacaggact
    29821 actactgaat gcaggagcag atcaaggaat tgcacgttta gcgggtaata ttgggtatcc
    29881 agcaagtggt gtagcgcaag aagcaaaatc agaagataaa attgtgatgg aattgtctag
    29941 cttccaactc atgggaatca cagatttccg ccctcatata gcggtgatta ccaatatcta
    30001 tgaagcacat attgattatc atggtacaag aaaagaatat gtcaaagcta aatggaacct
    30061 tcaaaaaaac atgacagaaa aagattatct gatccttaac tggaatcagt cagaactaca
    30121 agaattagcg caaagaacaa aagcacgcgt actgcctttc tcgacaaaag aagtgttgga
    30181 agacggtgtg tatgctgacg attactcaat atactataaa aaagaaaaaa taatggaaat
    30241 atctgaactt ggcgtaccag gtaagcataa tgtagaaaat gcactagctg ctatttctgt
    30301 agcaaaattg tatggtatat caaatgaagc tatacgagaa acgttgcatt ttttccatgg
    30361 cgtaccacat cgtacgcaat atgtaggaga aatccaaggc agaaaatttt ataatgattc
    30421 aaaagcaacc aatattttag cgactaaaat ggctctaagt ggatttgaaa cgtctaaggt
    30481 cgtgttattg gctggcggat tagacagagg aaatacattt gatgaattaa ttccttcatt
    30541 aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa gaaaagttga tggatgctgg
    30601 aaaaaaagca ggcatcgaga caatagtaac agccgattct gtagaacaag cagttccttt
    30661 agcattagaa aacagtacag atggtgatgt agttttatta tcaccagcga atgccagctg
    30721 ggatcaatac ccaaattttg aaactagagg aaacagattc atggaagctg tgaaccgatt
    30781 aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat tttggttaca ggcggaggaa
    30841 cagggggaca tatttatcca gctcttgcct ttgtcaatta tgtaaagact caagaaccga
    30901 atgctgaatt catgtatgtg ggagcaaaac gaggtctaga aaacaaaatc gtccctgata
    30961 caggaatgcc tttccatacc ttggagattc aaggatttaa acgaaaaatt tccatgcata
    31021 acatcaagac ggtccgattg tttcttaaga gtatccgtca agcaaaaaaa atcctcagtg
    31081 attttcaacc agatatcgtt atcggtactg gaggatatgt ttccggagcg gtagtgtacg
    31141 ctgcagccaa aatgggtatt ccaacagtta tccatgaaca aaacagtgta ccaggtgtta
    31201 ccaataaatt tttaagccga tatgtggatc ggatcgctct ttcatttaaa gatgcggctt
    31261 ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc tcgagcacaa gaagtagcag
    31321 atactaaaaa atcagatatt ttgaaaacat ttgatttgga tccagaaaag aaaacagtct
    31381 tgatttttgg cggtagtcaa ggagcactta aaatcaatca agcagtatca gtttttttaa
    31441 gtgaatatat tccttcttct taccaagttt tgtatgcttc aggagataga tattatgaag
    31501 agatcctaaa taaaataaat cctgaaatac aaaatattag tgtccagcct tatatcaaaa
    31561 atatggcaga ggtcatggct tccagtgatc tgctggtggg gcgtgctggt gcaacttcga
    31621 tcgcagaatt cacagctctg gggttaccgg cagtgttggt tcccagccca tatgttacga
    31681 atgatcatca gactaaaaat gcaatgagtc ttgttcacgc aggagcagca aaaatgattg
    31741 cagataatga actaacaggt gaatcactaa gtcagacagt caacgaaatc atgggtgatg
    31801 aagaattgca aaagcaaatg tgccgagctt ctaaagaaca aggaatccct gatgcttcaa
    31861 aacgtcttta tgacttagta aaacaaataa tcaaaaaata aaggcggtgg tcttagtgag
    31921 taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct gatgaaaaca atttgactcc
    31981 ttggcaaaag gctaaccgaa aatatctggc agaacaagag atgaaaaacc aagagccaaa
    32041 aaaagaagac ataacattag atacacccct ttcagattct gaagaagaaa aaacggttca
    32101 agaggaacag caagaagaaa gccagtccga gaaaaaaatc gaattttttg aagagatcaa
    32161 gcaaaagaaa aacggtggac catataacgg ttcttttttg aaccggctgc ccaatttaaa
    32221 aagtcaacgg cacaaagtac tatttcgtcg gttagctttg atcatcactg tattgggtat
    32281 accgctgatt tttttgatct attatgtttc tccttatagc aaattacaag cagttgcagt
    32341 atcaggcaat aagacagtca acagccaaga aatcatctct gatacaaaat tgtcattagg
    32401 agaaaatgtt tggggacagt attttcatcg atctacttat atcgatcgcc ttaaaaaagc
    32461 acagccaaga atcgaaacag caaatataca tttcaaaggg atgaatgaat tcgatttgga
    32521 tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac aatcaatact acccagtgat
    32581 tgaaaatgga acagtattag atgaaaaagt agcaaatcct acaaaaaatc tccctatctt
    32641 ggaagagttc aaagactctg caaaaatcaa agaattaaca aagcaataca atcagttatc
    32701 atcagaatta caaaaagcaa tttctgaaat caagtacacg ccaagagcaa gcaataaaaa
    32761 tctgatccaa ttgaatatga atgacggcaa tcaagttatt gtaaatatca ataatcttgc
    32821 gaaccaaatg aaatattatt cgcaagtagc caaagatatg gatgaaaaag gtgtgatcga
    32881 catggaagtt ggtatcttct cgcatcctta cggtagcact caagaaacgg aagaatcggg
    32941 ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat caaacaaacg aatcaagtga
    33001 agaggcagaa acgcaaaaag cacaaacacc tggatcgtct agcacaaacg gatcatctga
    33061 ttcaaatgat tcaagtgaaa caagtcaaat agaagataat ccaagtacaa ctccgtcaag
    33121 tttaaatgaa aattgataaa acaactttaa aaagtaagaa tgtactttct aaaaaccagt
    33181 aatttatggt agaattggaa gcagtgaata tatctattaa taagtatatt ttaaaacgga
    33241 cgctagttgt tcgcttgtat atggaatagt aagtaggagg gaatcccatt catggcaaaa
    33301 acaggaatgt atgtaggcct tgatattgga acgacatctg ttaaagttgt cgtggctgaa
    33361 tatatcgaca gccaaatgaa tattattgga gtaggaaacg caaaatcaga agggattaac
    33421 cgaggcatta tcgttgatat cgataaaacg gtccaagcga tacaacgagc agtacgacaa
    33481 gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg tcggtttgcc agcaaacatg
    33541 cttgaagtag aaaattgcca gggaatgatt gcagttaacg gagattcaaa ggaaatcacc
    33601 gatgaagatg tcagaaatgt ggcttcagct gcccttgttc gttcaatacc tcctgaacgt
    33661 caaatcgtgt caatattgcc acaagatttt actgtcgatg ggttcgaggg aatcaaagat
    33721 cctcgtggaa tgatcggcgt aagattagaa atgtacggtc tgttgtttac aggacctaaa
    33781 acgatcatcc ataacatacg taaatgcgtg gaaaatgccg gattaatcgt caacgaaatg
    33841 gtgattactc ctttagcgct gactgaatca attctttctg acggagaaaa agattttggt
    33901 acgatcgtca ttgatatggg tggcggccaa acaacaacgg ctgttatgca tgataagcaa
    33961 ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg tcactaaaga tatctctatt
    34021 gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa taaactacgg ggatgcatac
    34081 ccagaacgga catcaccaga tgaagaattt ccagtagatg tgattggtca gtcagaacca
    34141 gtgaaagtcg atgaacgcta cttgtcagaa attatttcag ctcgaatgga acagattttc
    34201 aacaaagcaa aagatgcatt ggaccaaatc gaagcattag agttgccagg tggcgtcgta
    34261 ttgacaggcg gagctgctag tcttcctggt gttgtggact tggcacaaga aatcttcggc
    34321 gtaaatgtaa aattgtatgt tccaaatcac atggggctga gaaacccggt atttacaaat
    34381 gtaataagta tcgtggatta ttcagcaaac ttgagtgaag tgtatcaatt ggcaaaaatt
    34441 gctgttacgg gtgaaacctc agcagctcgt caaatggtag ttgaacaaga aacaacaaac
    34501 acatatgaga gctatgaagc acctgaagaa acaatctacg atgagccgga accaaaagag
    34561 tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta caaatatttt tgattaattc
    34621 atcctgggag gaataagata tggaattctc aattgataat aatatcaatg acggcgcagt
    34681 aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct gtaaaccgaa tgattgaaga
    34741 aaacgttaaa ggtgttgaat tcattacagc caacacagac gtgcaagcat taaaaaattc
    34801 aaaagctgaa acagttatcc aattaggccc caaatatact cgtggtttag gtgctggttc
    34861 tcagccagaa gttggacaaa aagcggcaga agaaagtgaa caatccttac gcgaagcttt
    34921 agacggtgca gatatgattt tcatcactgc aggaatgggt ggaggaaccg gtacaggtgc
    34981 tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca ttgactgttg gtgtcgtgac
    35041 acgtccattc acttttgaag gaccgaaacg tggccgtttt gctgcagaag gtatcgctcg
    35101 tttgaaagaa aatgtggata cattattaat tatctcaaat aaccgtttat tagaagttgt
    35161 agataaaaag acaccgatgc ttgaagcttt ccgtgaagca gataatgttt tacgacaagg
    35221 tgtacaaggt atctctgatt tgatcacagc gccaggttac gttaaccttg actttgctga
    35281 cgtgaaaaca gtgatggaaa accaaggtac agcattgatg gggatcggtg tggctagtgg
    35341 agaagatcgc gtagtagaag caacgaaaaa agcaatctcc tctccattgc ttgaaacatc
    35401 tattgatgga gctgaacaag tacttcttaa catcacaggc ggtttggata tgacattgtt
    35461 cgaagcacaa gatgcttcag atatcgtagc aaatgcagca actggcgatg taaatatcat
    35521 tctaggtaca tctatcaatg aagaaatggg cgatgaaatc cgtgtgacgg tcatcgcaac
    35581 aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga ccagctagac aagcacaaat
    35641 gcaatcacct gctcaaaaga ctgtattaga tatggatcaa gctaaaccaa cttcttcaga
    35701 agaagaaaac agttttggcg attgggatat cagacgtgaa caaaatgttc gtcctagagt
    35761 tgatgattcc aattttgaca atatcgaaaa aaaagaattt gatacattca accgcgaaga
    35821 aacaaaatct catggcgatg atgaattaaa tacgccaccg tttttccgtc gtaaaaaata
    35881 aaggagagga agagcacatg attgctgaca acttgcagaa aatcaagcaa gaaattcagg
    35941 attcgtgtgc tcttgttcat cgtgatccga aagaagttac attagtagct gtaacaaaat
    36001 ctgttgaatc cgctcaagct gaagaattgg ttagagctgg cgttatgaat tgtgcagaaa
    36061 accgagtaga taaattatta gaaaagaaac aagatttatt tgattttcct gaaataaagt
    36121 ggcatttaat tggtaattta cagcgcagaa aggtaaaatt gattgtaaat gaaattgatt
    36181 actttcatgc gttggacagt ttaagattag cagaagaaat ccagaagcgg ttagagaaag
    36241 aattatcctg ttttattgaa gtaaatgttt ctggtgagga atcaaaacat ggaatttcac
    36301 cagatgagct tttaccgttt gtggaatctt tagctgcctg cacaaaaatc aaggttgttg
    36361 ggttgatgac tatggcgcct aaagaagctt ctgatgagca aatccataaa gtgtttggta
    36421 cgctgaaaga attgcgtgat cttgttcaag caaaaggtta tgcccatgca ccatgtagtg
    36481 aattaagtat ggggatgagt caagactttc aaattgcgat tgaagaaggt gcaacattta
    36541 tccgtgtagg aacagcgcta ttcaaagacg aataaaggag gaacaaaaat gccgctttta
    36601 aacagaggag caattgctaa ttttttcgga ttagctgatg aggaagagta cgaataccaa
    36661 gattatcctg aaacaacaaa aaaggctgta cagccaacta aaaaaaatac aacacagaat
    36721 caatcaactc attctgtatc tgctgcgaat actcatgaaa aacaaaaaac ggcgacttca
    36781 cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga aaaaagtaat tgaactacat
    36841 caatcaccta caaaaactgt agcgaaagaa agaacagctt caagaacctc tacacaacct
    36901 aaagcaaatc ttgctggaaa aatcactgta atggaaccaa gagcttattc tgaagctatg
    36961 gcgattgcta aacgaatcat tgctggagaa gcaaccttag tgaattttca tttagtagaa
    37021 gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa cagtttatgc actagatgga
    37081 gatatcaaac gtgttggaga tgagattttt ctttgtacac cgactgggat ggagattgac
    37141 agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg atctgtaaga ggaggaacta
    37201 cgatttatac tattgcacta ttacttcatt tacttcaacg ggcagcctat ttctatagca
    37261 ttatcttgat ggtatatgca ttgctctctt ggtttccagg cgggtatcaa tcagggctag
    37321 gacgattatt agctaagatc tgcgaaccgt atatcagttt gttcgatcgt ctgcctttga
    37381 ggattggacc cttggatttc acaattttag gggcaatcct gatcttgaac ttagccgtac
    37441 aaggtttagc acagatcgtt atatggataa ctgtccattt agtttattga ttgaagcaac
    37501 aaaatcttta ttctcagaat agataggagg ggaaaatttt ggatgcaaat gtataccagc
    37561 actttcgggc agatgaacgt acatttatcg attcggtagg cgattggatt gaacaggttc
    37621 aaggacaata tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc tatattttag
    37681 aaacactgat tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga tatgaacagg
    37741 cagaaagaaa aagatgtttg attttccccg attattacga acctgtagag gaagattttg
    37801 aaacagcatt gtatgaaatc cattatccta gcaaatttgc cagcctaagt catgggaaaa
    37861 tccttggaac tttagtggga acggggatca aacgctctta ctttggcgat atcatctcgg
    37921 acggtgatcg ctggcaagta tttattgcaa atgaggtgga tcattttgtt gctgcacaag
    37981 taacaaagat tggaaaggtt gctgtacgct tggaaccaat caaatacaca gaaatgatta
    38041 tgccgaaaga tagctggaca caagaaagag gaacagttag ttccatgcga ctagacagtg
    38101 tgatctctga gatttataat atttccagac agcggtccaa gcagctgatc gaagctggta
    38161 aagtaaaagt gaactggaca gaaaatacaa gacctgattt tgtacttgaa ctattagata
    38221 ttatttcagt cagaggttat ggcagagtcc agatccaaga acttgagggg aagacgaaaa
    38281 aagaaaagta cagggtactg ttcggtgtat tacgtaaata aaataaaaag gtggtttact
    38341 catggcttta actccattag atatccaaaa taagacattc ccgacaaaaa tgcggggata
    38401 taatcaagac gaagtagatg atttcttaga tttagttgtt cgtgactatg aggaattgac
    38461 tcaaagaaat cgagaattag aaaaagcagt caaacattct gaagaaaaac ttgagtactt
    38521 caatgaatta aaagatgctt tgaaccaatc aatcatcgta gctcaagata cggctgataa
    38581 agtaaaaaca agtgcaagca aggaatccga agtgatcgtt acttctgcac aaaacaaagc
    38641 agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg atgcagaaga
    38701 aaaagcaaga aaaatcttga cagatgcaac agaaaaagca agacagcttg cgacagaaac
    38761 tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga tgcttgagtc
    38821 tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat tctccagcta
    38881 cgtgactgac agccatgaag tgatcaaaga agttttagca aaacagcttg acaatgaaaa
    38941 tgatactgat gtaaactcag acacaggagt catagaagca cctatgacgg catttgatac
    39001 acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga acagaaaagc
    39061 actttgttat tcttaaagcg agccgatgag agtggaagtt cggtaagaca ctgcatggtg
    39121 aaagatcctc tacttgagac ctgcttttcc gaaattagag taagaagcag cggctgacct
    39181 cgttattggt cacagagagt ttgcactaga tagatgcaac taaatttggg tggtaccacg
    39241 gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa ataaaggagt
    39301 gtatggttga atgaaaatga aagaaacctt gcaattagga aaaacaaaat tccctatgcg
    39361 tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa atcgaatcta
    39421 ccaaaaaaga caagagttga acgaaggaaa acctagcttt gttcttcatg atggacctcc
    39481 atatgccaat ggtaatatcc atttaggaca ttcattgaat aaaatcagta aagacattat
    39541 cattcgttca aaatcaatgt ctggctatcg tgcaccatat gttccaggat gggatacaca
    39601 tggattgccg attgaacaag tattggctaa taaaggcgtg aaacgtaaag aaatgtccat
    39661 ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc caagtagata agcaacgagc
    39721 agatttcaaa cgcctaggag ttgcaggaga ttgggaacat ccttatgtta cattagatcc
    39781 ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag gctatattta
    39841 caaaggattg aaaccaattt actggtctcc gtcaagtgag tcttctttag cggaagctga
    39901 aattgaatat aaagatgtaa aatctccatc tatttttgta gctttcaatg tcaaagacgg
    39961 caaagatatt cttgatgaag atacagcttt tgtcatttgg acgacaacac catggacatt
    40021 gccagcaaac caaggtattg ctgtaaatcc aaaatttacg tatgtattgg tggaagccga
    40081 tggccgtaag tttgtcgtag cgaaagattt gctagaaaca gtccaatcag aaatcggttg
    40141 ggaaaatgta acagttctaa aagaatttgc tggacaagaa atggaatata tgactgcaag
    40201 tcatccattc tatgaacgtg aatcacttgt gattttaggc gatcatgtta ctttagacgc
    40261 tggtactgga ttagtccata ctgctccagg acatggggaa gatgactata tcgctggtag
    40321 caaatataaa ctatctgtag tgtcacctgt agactcaaaa ggattgttta cagaagaagc
    40381 acctggattt gaaggtatct tttatgacaa agcaaatcct atgatcactg atctattaaa
    40441 agaaaaagga gctttgctga agctagattt ctttactcat agttatcctc acgattggcg
    40501 gacgaaaaag cctgtgatct atcgggcaac tccacaatgg tttgcatcta tcgataaatt
    40561 ccgtcagaat attttggatg aagtagaaaa agtagagtgg gttatcccat ggggtaaaac
    40621 acgtttgtat aatatgatcc gtgaccgcgg cgattgggta atttcccgtc agcgtgcatg
    40681 gggagtacca cttccgattt tctatgctga aaatggagaa gcaatcatca caccagaaac
    40741 aatcgaacat gtggcaaaat tatttgctga acatggatca attatctggt ttgaaaaaga
    40801 agcgaaagaa cttcttcctg aaggatttac ccacccaggt tctcctaatg gcgaatttac
    40861 aaaagaaaaa gatatcatgg atgtctggtt tgattctggt tcttctcatg aagctgtttt
    40921 acgtcagcga ccagaattat cattcccagc agatatgtat ttagaaggat cagaccaata
    40981 tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct atcaatggtg tagcaccata
    41041 caaatcagtt atttctcaag gatttgcttt agatggtgaa ggccgtaaga tgagtaaatc
    41101 tttaggaaac atcattgcac cagaaaaagt gatcaaacaa tttggtgcag atatccttcg
    41161 cttatgggta tctagtgtag attatgaagc agatgttcgt gtttctatgg atatcttaag
    41221 tcaagtggca gaagtatatc gtaaaatcag aaatacgatg cgtttcttat tagcgaatac
    41281 agaagatttc gatcctaaaa aagatactgt ttcgtacaat gatttacgtt ctgtagacaa
    41341 atatatgacc gttcgattga accaagtaat caaagaaatc cgagaagaag ggtacgacaa
    41401 atataacttc atgcatattt accgtacagt tatgaacttc ttgacggttg atctatcgtc
    41461 attctacctg gattttgcta aagatgtagt ttatattgaa gcagaagatg attatcaacg
    41521 tcgttgcatg caaactgttt tctatcaaac agctgtagct ttgacaaaat tattgactcc
    41581 tatcattccg catacagcag aagaaatctg gtcattcctg aaagaagaag aggagtacgt
    41641 tcagttaagc gagtttcctg gttatgaaga gtttgcaaac caagaagaat tgatggatac
    41701 ctggactgct ttcatggact tccgtgataa tgtcttaaaa gcattggaag aagcaagaaa
    41761 cagcaaattg attggtaaat ctttggaagc aaaactaact gtttatccaa aacaacaagt
    41821 tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa ttattgatcg tttcaccaga
    41881 ctactttgaa gttatggcag cagatgaaca agtgccagat aacgctatgg tatttgatga
    41941 tgttgctatt acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc gccaagttcg
    42001 taaagatgta ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg caaaaatcgt
    42061 tgaagacttc tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa agggaaaagg
    42121 gggtgtgaca tgttctttgt cacaccccct tttgttatca atcgtttaat tttccacgtt
    42181 cacgatacca gttatctggt tgccagatcc attcgaatcc gtctttttca agtagttcga
    42241 aagctgcttt aggtcccatt gatccagctt tgtagttggg aaaatccggt tgtgtttggt
    42301 cccaagcttt tcgaatgaca tccacgatat gccaagattg agcgacttct tcccaatgag
    42361 agaagttcgt tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa gcttctgggc
    42421 tattttcaac catttctgca ctatttctaa attccaattt gattggttcc gtttcaaatc
    42481 cttgccctac ttctttccca ttcaaggata aagaaaatcc ttcagttggc tgaatataga
    42541 tcgttagaac attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca gatgttttga
    42601 atacattaac aggaacttgt ttgaatacga tgttgatccg agtccctttt tcagtcaaac
    42661 gtttgcctgt acgtacatag aaaggaacac ctgaccaacg gaagttatcg atagtgaatt
    42721 tgcctgcgac gaatgtttca gtctctgatt gagcatccac tttgtcttct tcgcgatagc
    42781 ccttgaattg tacaccatcc aattcacctt gagcatattg cccgcgaacg aagttttcta
    42841 acacttcttc ttcgttataa atgcggatag ctttcaatgc tttgatcttt tctgtacgga
    42901 tttctttttc ggaaaatgct actggcggtt ccattgcaag taatgctaca acctgtaaaa
    42961 tatgattttg caccatgtct ttaagtgcgc cactatgatc gtagtagccc ccgcgatctt
    43021 ctacaccgag gttttcgcca aaagtaattt ggatattgtc gatatagcga ttgttccatt
    43081 gagcctcaaa aatattattg gcaaaacgga tagctgagat gttttggatc atttcttttc
    43141 ctaaataatg atcaatcctg aaaatatctt gttctgggaa tacttctctg atttgctggt
    43201 tcaattcaaa agcagactgg taatcagaac cgaacggttt ttctatgatc aaacgattga
    43261 agccattttt tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt ccaaaaaatt
    43321 gaggtgccat agccaaataa tagacacggt taccgtttaa ttgatagtgt ccatctaatc
    43381 ggtcagacaa ttcttttaat gtctgataat gcgttgaatc atttacatca tgggattgat
    43441 agtaaaaatg gcttgcaaag gtagttgctt cttcatctga aggattcaat gcacggattg
    43501 tttctttgac aacttcacgg tagtgctcat cgctccaggg acggcgagcg gttccgatta
    43561 cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac gggtacaatt
    43621 tacgctgggc taggtcacct gtaccgccga atatcgtaaa caatacattt ttttcattag
    43681 acatgtggat acactccttt atattttatc gacaaaaatc gtgctggccg ccttaaagct
    43741 gacggggatt ttctttctat cattttggat agtgatcggt ccttcataag ctgcgatgtc
    43801 aacgatctca tattcttcgt tgatattcag gtcaatagtg actaagtaat ccaacaattc
    43861 tttttcatca agcactcgtg cgatacgcac ttttgtaccg acaggatagg actccaatgt
    43921 ttgtcggcgt tcttcatgga caacttcctc ttttttagga atcgatcctc catgaggaca
    43981 atgttcagga tgattcaaat agttttctaa atgatctgca agatgttccg aagtcacatg
    44041 ttccaatact tcagcatcat catggacttc attccagcta tagtctaaat gttcaactaa
    44101 aaaaacttcc cataagcgat gtttgcgaat cagactgcta gctttttgca gtccgcttgc
    44161 agtcaattgt acaccttgat aaggggaatg ttctacaagc tcttctttca caagtttaga
    44221 aatcatttca ctgacagatg cagctgagac atctagacta gatacgattt gtttattgtt
    44281 gactttcgtt gtatctccac ctagttctaa aatgattttc aaataatctt ctcgatttgg
    44341 tgtcatattc gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt aacaaaaaaa
    44401 acgttaattg actatttttc taaataaaaa acaaaggatt tttagacgtt tttacaacaa
    44461 aaaacgaaag atgatagaga agactcattc tttcgtttat aaaggatata tatatacatt
    44521 gggagggatc tatttttcag ataacaactt atatgaagtg tctgcagatt cgtcataaag
    44581 agtttgttcg ggataagaaa agataagata aaaaccatac tgatcacgta cgactaaacg
    44641 atattcatct atttctacca cagatgtgcc aagagactga ccgttgatca aaaacgtgta
    44701 atcagatgta atctgtaatt gttgtttttg ctggctatca ctaaagaacc atgtaccttg
    44761 atactttgag agtccttctg acttttccgc ttgttttttc tttttcaagt aaataaaact
    44821 tgagagaacg gtaatgagtg aaccaagaaa atagatcatt ttttttatca ccaaatcact
    44881 cctttatgag aaaataatcg catagacata ttctttcttc tttatttcat tgtaccgaat
    44941 aaaagctaaa atttctctaa gactttcgac tggttttttt actgtttttc tacaaattat
    45001 gataggatac atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag
    45061 ctttttttat tgaccgcccg aatcggttta tagcacattg tatgaaaaaa gacggagaaa
    45121 taattattgc gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata ccaggcgctg
    45181 aagtggctat tgtatttgct cctggaccta aacggaaaac cgcctatgat ctgattgctg
    45241 taaaaaagaa gggtgactgg tttaatatcg atagtcaatt gcctaatcgg ctggcaattg
    45301 atggtatttt agacggcacg atccttttac caggattaac tggtccattt acggtataca
    45361 agcgtgaagt aacttatctt cactccaaat ttgatattta tttagagacg gtggtcggac
    45421 aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc
    45481 ctgatgcgcc cacactcaga ggattgaaac atataggaga attaacgtat gccgcacaag
    45541 atggttatgc cgcttatgtc ttgtttattg ctcagttcga acatcttcat ctggcaacga
    45601 tccatgaaga aatgcaacca gcactagctg atatggttcg acatgcgcag caaagcggag
    45661 ttcaaatcct cgcctataat tgtcaagtat cggcagatga ggtaactgtc aagcaggcca
    45721 ttccatttga tgtagatatc ccttttgaag atcctataca atagaaagaa ggtaaaataa
    45781 tgatcaactt aggtattatc ggaacaaatt ggattactca tcaatttgtc caagcagctt
    45841 taagcacaaa aaaatatgaa ttgacagcgg tatattctag acatttggaa aaagcacagg
    45901 aatttggttc tcattatgaa ggggatatcg aattcgcaac agatttgaag acttttttca
    45961 atattactca tatggacacc atttatattg cttctccgaa tagtcttcac tttagtcaag
    46021 ctaagcaagc aattttggca ggaaaaaatg tgatagttga aaagccagct ttttcgacgc
    46081 caaaagaaat ggatgaaatc atccatttgg caaatgaaca caatgtgtat ttttttgaag
    46141 cagcaagaaa tatccatgaa tcaggtttta aaaagattgc cgattactta ccattaaaag
    46201 acgagataat cggtgcaaat ttcagctata tgaaatattc ttcgcgctat gaccaagtat
    46261 tagatggaga agaaccgaat atcttctcga ctcatttctc aggtggtgca ttgatggatt
    46321 taggtgttta ccttgtatat gcagctgttg cttggtttgg aatgccaaaa gaagtccatt
    46381 atttcccaag aaagatttct actggtgtag atggtcttgg atgggggatc ttacgttatg
    46441 agaattttga tgtatcgatc cagccaggta aaatcggtga ttcttattta ccgtcagaga
    46501 tttattttga ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa caagcacaat
    46561 ttcatgatcg cgcgcataaa gaaatcattg atttggcggt aacagctgca gaaaatccaa
    46621 tggcagatga agcagaggat tttgccaatg tcatggctca tccgaaagat ccagcttggg
    46681 gattgttgta tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg atttatgatt
    46741 taagaaaaaa tggcggcatc aaattcgatg cagataatga ggaagatttt taatgaacga
    46801 atttgtacaa gtatttcccg aaaattggca gaaaaaatat gaggataaag ggtttatctc
    46861 accttctctt attcaacgag cagtttttca gccactaagt aataaacaaa gtatagtagc
    46921 ggtttctcct acaggttcag gtaaaacact ggcttatctt tggcctctac tactaaacgt
    46981 ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct tctcaagaac tagctattca
    47041 agtagcagat gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc aatctttggt
    47101 gggcggagcc aacgtcaaac gtcagattga gggtttgaag gaaaaaccag aaatcttgat
    47161 tggtacacca ggaagaatat tggaattgat gaaagcaaaa aaaataaaag cacatcaagt
    47221 gaaaacaatg gtatttgatg aagcagacca gctatttgac gctgggaaca gccaaatcat
    47281 tgatcaaatt cttcatcaag ctccaacaga gtatcagcta gctttttttt cagcgacagc
    47341 agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga caattgacgt
    47401 gacagcagag gacgattctc gaaaaggact gagacattac tttttacgtg taccgatacg
    47461 aaagaaagat gactacttga gacgtctcac gcatatagaa gattttcatg gtctgatttt
    47521 ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc
    47581 ggtaggaagt ctggcctctg atcagaacaa gctactccga aaagcagcgc tagaacaatt
    47641 caaaaagaaa atggtcagag cacttttgac cacagatgtg gcggctagag gtttagatat
    47701 tacaggtctt ccttatgtag tgaatgcaga agttccgtta agtgaagaag cttatttgca
    47761 tcgttcggga agaactggtc gaatgggtaa tgaaggaata gtaattacac tcgtacaaga
    47821 taataatcta agagatttaa aacggctttt acgtccatca aatataacac ttgaagagat
    47881 ttttttatac ggaggtaagc tgcaaactga gcagccaacc aaagaagatg cagctcctgc
    47941 agtaaataaa aagtatgcga aaactttttc tggaaaggag cagaacccag aatataacaa
    48001 agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag
    48061 gcgcaaataa gcgccttttt atgctatgat tatgaaaacg ggaacaaaat tggttgccag
    48121 aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc agctattgac agaaaaccaa
    48181 caaggattga cagcaagtga tgtagctgag cgcttaacta tcgatcgaag caatgcaagc
    48241 cgatatttaa gtgaactata caaggcccat catattgtaa aaaccgctgg ccgaccagtc
    48301 gtttactctt tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg
    48361 caagtgactt ttgaaacact cgttggagaa aatgattctt taaaagtcag catacagcag
    48421 gcaaaagctg cgattcttta tccaccgcgg gggttgcata cgattatttt tggagaaact
    48481 ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg
    48541 ctttctgcag atgcgccatt tgtttctttc aattgtgcgg attatgctca aaatccgcaa
    48601 ctgctctttg gacacatctt tgggatcaaa aaaggggctt acacaggagc agctcaagat
    48661 agtcccggtc taatagccaa agcagacggc ggtatattgt ttttagatga gatccaccgt
    48721 ttaccgccgg aagggcagga aatgctgttt actttcattg ataaaggtat ttaccgtccg
    48781 cttggagaaa gtgcacaagt acatgaagca tctgttcaga tcattggggc gacgactgaa
    48841 tcttcagaaa gtttcttatc tacgttcaat cgacggattc ctatggcaat caccttgccg
    48901 aatcttgctt cacgttcact ggatgagcgt tatgaaatca tctctctttt tatcaaacaa
    48961 gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag aagcgattct tgccttcatg
    49021 ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact agtctgtgca
    49081 aaatcttttt tacattaccg tactcatcat gagaaaaagc taatcattcg aaaagaagaa
    49141 ctttcccttc aagtacaaaa aggattactg aaaatcaagg aaataccgga acgtctcgac
    49201 cgattcatgg acagcaaaag tcagtatttg acatttgaac caggatttgc tgatgttgtc
    49261 tggtcacaag atccagaacg aaacatgcag gtctataacg atattgagga aaaagtcact
    49321 tctctttccg aaaccggtgt ggagaacatt gatctagaga ccctcatttc aaaagatgtg
    49381 gatgcttatt tccagacata tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt
    49441 ttgccagatg aaatctggcg gttaaccaat caattgtatg atgtggctga aaaacggtta
    49501 gaccgtattt ataatgaaaa agctcgtttt gcttttgctt tgcatctgca aagtacattg
    49561 gatcgtgtga gagaaggtca tatgatcgtc cacccagatt tgaataacgt acggaaaaat
    49621 tttaagaggg aattccaggt agcaatcgat ctttcaacga tcattgaaga agaagaacaa
    49681 gttgagattc cttttgatga aatcggcttt attagtatgt ttctttctat caatctagga
    49741 gaaagtgagc ctgttaaaga aaacaaagta gatgttctca tattgatgca cggcagacag
    49801 actgcttcaa gcatgttgga gatggcacaa gaactattag gaacaaaagt agggaaagct
    49861 tttaatatgc cactggttat ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc
    49921 aaaaactatc aagagcattt gacgaatggc ctactcttgt taacagatat gggatcatta
    49981 aacaccttca gtgatttgat ttatgaagag actggcattc ggacgaaagc tatcacgatg
    50041 acaagtacca tgattgtgat agaagcgata cggatggcaa gtgttggaag aagcttagaa
    50101 gatatttatc aaaatatcca gctttccttt gaatcgatcg tacgtgatca gttcaaagcg
    50161 gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa
    50221 ggagtagctg ccaaattata tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa
    50281 ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa aacacatcga tgccttattg
    50341 gaagaatatg agatcaaagc aattgcggga acagttgatg ttgattatca aaatatccca
    50401 tttttttcag cctatgacgt ttttgatgat gaaaaactaa atattttaaa gcgtatagct
    50461 gatgatgaga tcccgacagc taaaattgtc gattcacttg ctggatcatt aagcgatgtc
    50521 ttttctattg atgaactgat ccgttcattg caaaagacag ttcagcagat ccagaccgat
    50581 ttgtcgatta tcgtggaacc tggagtagat actggaatcg ttatccattt agccttcatg
    50641 ttagacggat tgatcaaaca agaaagccat cggcaatttg agaaactgtc tattttctca
    50701 aaaatttatc gtttggaaat ggatatcgtt cgggcaaact tgatggcact agaacgaaaa
    50761 tatcaggtaa aaattccaga agatgatatc gcatttctta cacaaatgtt tcttgaaaat
    50821 aaaataaaaa aatgaacaca ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac
    50881 tataaaaaag agctaaaaag catgaaaacg ctatttcagc tctttttttt attggcatgc
    50941 ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac
    51001 aatcatgtta gtttgttcag cagggatgag tacgagttta ttggtcacaa aaatgcaaaa
    51061 agcagcagaa gaaaaaggga tggaagccga tatttttgcg gtctctgcct cagatgcaga
    51121 taataattta gaatctaaga atgtcgatgt attattatta ggtcctcaag tacgtttcat
    51181 gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt ccattagatg tcatcaatat
    51241 gcaagactat ggcatgatga acggagaaaa agttcttgca caagcagaaa atctaatgaa
    51301 ataaatcaag tatgcagttg ggggtataac aacgtggaag atcaacaaaa tctagaagcc
    51361 atcatggggt taatcatgtt tggcggtaat gcaaagagcg atgcaatgga agcgattgcg
    51421 gctgctaaaa tcggtgattt tgagttagcg gataggaaaa tcgctgatgc agaagaatca
    51481 cttatacaag cgcatcatgc acaaacagac atgttgacac aagaagcaca aggaaatcat
    51541 atgcaagtga cattattgac tgttcacagt caagatcact taatgacatc tattgcgttt
    51601 actgatttag caaaagaaat catcgaactt tatcgtcgcg tatacagcga ataaatacaa
    51661 cttctttttt gaacggaaga agaaaaattt ttgaacggat tttttatttt gttataagga
    51721 gaactctctt tatataaaaa acttatagga ggaagtaaaa aatgaacgga ctatcaagtt
    51781 ggatggagaa atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa catttagttg
    51841 ctttgcgtga tgcttttatc ggcacactgc ccgcaacaat ggcgggatca gtcgctgtaa
    51901 tgatcaatgc aattattcgt gatttaccgc aacagtttaa cagtacctat gctgacagtt
    51961 tagcagcaga caaaggaatc tttgccgtat tgaatgtcat catcggaatc aatggttttg
    52021 tctggaacgg tacactagca atcgccggat tgatctttgc cttttcttgg gggtataatc
    52081 ttgcccgagc ttatggagta aatgatctag ctggggggat cgttggttta gctaccttga
    52141 ttcaaggtat ttcctttggt ccagcattat cagcagcatt agatgtgaat gttccttcaa
    52201 atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac gattgcagat ggcacactgt
    52261 cagttggatt atggggttgg ctaaaactag atcatttgaa tggaaacgct tattttacag
    52321 ttatgatcat gggagctatt tcagtcatta ttttttgtaa attgatgctt gccaacatta
    52381 cgatcaaaat gcctgattca gtaccaccag cagtatctaa agcatttgca gccattttac
    52441 cagcgacgat cgctttatac gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg
    52501 atgggcaact gatcattgat ttggtgcaaa aatatattgc agagccattc ctaggattat
    52561 ctcaagggtt gggagcagta ttgatcgtca cagtatttgt tcaagtattc tggttctttg
    52621 gtattcatgg accaaatgta ttggcaccag tgctagaggg aatctgggga caagcacaac
    52681 taatcaatat tgatattttc caaaaaggtt ataacggaaa aactggtaca ccagccgtgt
    52741 tagatgcaat cgaagatgga aaagcttata tgtgggtccg cggatcgttc gatgcttttg
    52801 catggtttgg cggatcaggc ggaacgatcg tcttactgat agctatcttg attttctcta
    52861 aacgtgcaga ttatctaaca gtagctaaat tatccattgg gcctggtatc ttcaatatca
    52921 acgaaccagt gatgtttggt ctgccaatcg tattgaatgc aatcatgttt atcccattct
    52981 tgattgctcc agtagtagca acagcaattg gatggacagc tacttatctt ggcctagtcg
    53041 ctccggtatc gcagcaagtc acatgggtag taccgccgtt cttattgtca ttcttagcaa
    53101 caggagcgga ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg actttcttga
    53161 tttgggcacc atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat
    53221 aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa ttttcgagga
    53281 ggaactgaaa tgattacaat tgccagtgca atgcaacaag atgaaatcaa agaattgtta
    53341 gaaggataca atgaagaagg aatgactttt acatttaaag aaaaacaagg aatcaagctg
    53401 ttctttgaaa caagtgcaga agatccggaa gttgcggcta aaaaagcaaa ggaactaatc
    53461 aaagcacagc cttggggaac agtcctttat tttcaatcaa cagctgttta gaaaatggtt
    53521 taaaaagata ggatggttga gatgataaaa aacggtttat ttttatgtag tattggttct
    53581 ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt atgatttgta tagtatggtc
    53641 acaggtcttt ttctaacagg attaggaatc tatttcttca tgaaggggaa aaaaagagaa
    53701 gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt
    53761 ttttttacga taaagttatg gactctgtcc ttttcgatgt acgtaaagcg accggcaaat
    53821 cagtaactag aaaacagctg aaaaatttgg agtacgtcaa acaattctcg caaaataacc
    53881 gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt cgtacatcga
    53941 caacgaagaa tgaattcgtc gttcattatc aaatcaaacc attggacgat tcgacctgtg
    54001 aggttcatta cacagaaaaa atggagtctt atggcatgct tcaaaagttg aatgatatgc
    54061 ttttaggaac aatcttgatg tattttaaac ggagacgttt caaaaaaatg ctagatatga
    54121 tcgaagcaac gtattaaatc aatgaaacat ggtactattt agttattgaa aaatgaagta
    54181 agctagaagg aatttttcat aggaagatag atctttcctg cgaatcctca ctagcttttt
    54241 tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat cttcgattga
    54301 ggaaatgttt gatttagcca cttatgcatt taatggggca gatacacctg aaaggcgaga
    54361 acgttttcgt acacttgcgg agaattcttg gaattacgga ttttttgatg aggaggatcg
    54421 gctaacaagt caagtcatgg cgaccccttt tccagtgaat ttttacggac aagagtacct
    54481 aatggccggc attggctatg ttgcttctta tccagaagca aggggacaag gcgggatcaa
    54541 tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct tatcttatct
    54601 ggcgccattc tcctatccct tctatcggcg ctatggctat gaacaatcat ttgataaaat
    54661 cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa
    54721 gcgtatggat tttgaagatg caaaagctgc aatcagaaga atttatgaac agatgccgga
    54781 aaaccaacga gctggtctga agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa
    54841 aggcaattac cagtttgctt tgcattttga tgataatgag gaagcaacgg gatatatcgt
    54901 ttatcagctt gcaacgtctt cctttattat tgcggagtgg ggatttttgg atcatgatgc
    54961 tttttgcagc cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa catttgaata
    55021 ttcatgcggc tacggcggta atgatcaaaa ttatctatta gaaaatccat ttgcttctgt
    55081 aatgatcaca ccttatatga tggcgaggat cgtagatatt tctttgtttt tggaaaatta
    55141 tccatttaaa aagagacaag ctgcatttgc cctacaaata gaagaagatc attatgcgaa
    55201 atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt
    55261 agaacaaaca aaactcccaa tcgttcgagg aagtatccaa catatcacgc agctattatt
    55321 agggtatcgt aaagtggaag agttggtatt ccaagaaaga atacaggtga caaaagaata
    55381 tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca attcttaatg actatttttg
    55441 ataaaaaacg ggtgatgaca atatttttgt caccaccctt ttgtttttca tttggcattt
    55501 gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt gttttctttt
    55561 gttttagggt tttctttttt gtggtcttgg attgcttctt tcgtaatctt ttcccgattt
    55621 tttttagatg aatctaagcg ttctgcgtat actttttctt ttggcttatc attcattgga
    55681 aaggacctcc ctacattatt tcattctacc tctagtttat catctgttaa ggactagtgg
    55741 aaacgttttg cctaacttat aagctgattt ttttagcaga taagcttgca agtaaaaaac
    55801 aaacaagcta caattgattt attgaaattg ttcctgtagc tcagtaggat agagcaatcg
    55861 cctcctaagc gataggtcgc tggttcaatt ccagtcagga acgtacaaat aacccctgta
    55921 tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct aaactgctat
    55981 gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat
    56041 gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa ttgattacta
    56101 aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag attcgaatta
    56161 aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt tcttaactct
    56221 agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga tgacttttta
    56281 ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa agtaccatat
    56341 aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct tagatccttt
    56401 attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc ccaatcgcca
    56461 tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc catcttgtca
    56521 gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc tgctaattct
    56581 tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc tagtgctaag
    56641 tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga ttgagaaata
    56701 ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc atctgtatca
    56761 agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaacaac ttttccatct
    56821 ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg ctttgtactt
    56881 gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc cgtcggcata
    56941 tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca aagatggttt
    57001 ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgaagggc agaaaattgt
    57061 tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt ccatgctaag
    57121 acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa tgcctcaact
    57181 tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc agccgcagcc
    57241 acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat tagtgctttg
    57301 ccattcgctg attgataaga cgctactcga tggctatcta caccagcaaa tgcatcggga
    57361 tcggcagaaa caacgctgat cctgcttgct cctttttcaa gccattcatc cgcttgatcg
    57421 attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag aaattctctt
    57481 tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt ataagcttct
    57541 tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac aactgtgtgt
    57601 cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt tagaagttca
    57661 ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta actattagca
    57721 tgataccatt taattatgat tcaaacaatg aggctttaaa taaaaaaagg aggttatttt
    57781 tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt gaagaaaatc
    57841 aaaaactaat tttgcatatc aaaaaaacat gttacaatga aattaatcaa tggtggaagc
    57901 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
    57961 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
    58021 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
    58081 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
    58141 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
    58201 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
    58261 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
    58321 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
    58381 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
    58441 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
    58501 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
    58561 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
    58621 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
    58681 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
    58741 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
    58801 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
    58861 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
    58921 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
    58981 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
    59041 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
    59101 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
    59161 taccaaaaca tcattgaaaa gaaaataaaa cctaaagaca cctggggaat gtcatttggc
    59221 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
    59281 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
    59341 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
    59401 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
    59461 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
    59521 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
    59581 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
    59641 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
    59701 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
    59761 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
    59821 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
    59881 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
    59941 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
    60001 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
    60061 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
    60121 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
    60181 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
    60241 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
    60301 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
    60361 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
    60421 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
    60481 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
    60541 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
    60601 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
    60661 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
    60721 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
    60781 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
    60841 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
    60901 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
    60961 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
    61021 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
    61081 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
    61141 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
    61201 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
    61261 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
    61321 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
    61381 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
    61441 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
    61501 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
    61561 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
    61621 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
    61681 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
    61741 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
    61801 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
    61861 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
    61921 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
    61981 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcgtat
    62041 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
    62101 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
    62161 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
    62221 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
    62281 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
    62341 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
    62401 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
    62461 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
    62521 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
    62581 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
    62641 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
    62701 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
    62761 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
    62821 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
    62881 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
    62941 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
    63001 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
    63061 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
    63121 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
    63181 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
    63241 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
    63301 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
    63361 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
    63421 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
    63481 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
    63541 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
    63601 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
    63661 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
    63721 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
    63781 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
    63841 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
    63901 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
    63961 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
    64021 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
    64081 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
    64141 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
    64201 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
    64261 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
    64321 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
    64381 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
    64441 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
    64501 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
    64561 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
    64621 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
    64681 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
    64741 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
    64801 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
    64861 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
    64921 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
    64981 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
    65041 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
    65101 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
    65161 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
    65221 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
    65281 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
    65341 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
    65401 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
    65461 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
    65521 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
    65581 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
    65641 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
    65701 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
    65761 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
    65821 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
    65881 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
    65941 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
    66001 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
    66061 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
    66121 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
    66181 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
    66241 agagaacttg gccggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
    66301 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    66361 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    66421 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    66481 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    66541 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    66601 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    66661 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    66721 agtaacgagt atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    66781 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    66841 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    66901 attgatctta gaaatgatgg gccaaccaaa agacgcatac gaccatgtaa acgaccgcgc
    66961 agggcacgat ttgcgttatg caatcgattc gacaaaattg cgagaagagc taggttggca
    67021 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    67081 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    67141 ataatacaga ggaaatttca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    67201 tgtctctttt ttaaaataaa aatcaaggag cggataacca tatgttttta ttaactggag
    67261 gaaatggaca attaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgccatatg
    67321 tttcaacaga tgcaaaagac ttggatatca cagatgctga gaaaacaatg gcttatatta
    67381 cagaattaaa acccgatgtt attttccatt gcgcagctta cacagcagta gataaagcag
    67441 aagaagaagc aaaagaatta gatgagaaaa tcaatgtcga tggcacaaga aatgtagcac
    67501 ttgcagcaaa agctgtgaat gcacaattcg tctatatcag tacagactat gttttcgatg
    67561 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    67621 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    67681 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    67741 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    67801 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    67861 atcatttgtc gaacgaaaat agctgttctt ggtatgagtt cgcaaaagaa atattgaaag
    67921 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    67981 cgcagtattc agtcatgagc ctgaaaaaga cagaagcgtt aggttttgtc ataccaactt
    68041 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    68101 tttatcaaat agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa
    68161 gaaaagccaa aagcgaatca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc
    68221 cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt
    68281 tcacttggtg tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg
    68341 atttttggac agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac
    68401 ctctcacaag ctgaagagga cttttcgttg gtgaacgagg attattcatt aaacggacaa
    68461 aacatgatca ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc
    68521 aactggggat tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa
    68581 ttagtcgtca tggtcttgct tggattcgaa gtactgatga tattaacgaa gaggaaaaaa
    68641 gctctatcgc taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc
    68701 atgcaacatg tgggcgattt gattttcttt actttgggac tgatgattgc tttttatcat
    68761 tatttctaca atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt
    68821 ttcggtattg ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg
    68881 ctgatttttt attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg
    68941 ttggatgtag tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc
    69001 tggctaacgt caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg
    69061 gtaagtactg gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa
    69121 attccttttg aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt
    69181 tttccaactg tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa
    69241 aagttactgg gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt
    69301 gggttgccaa aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc
    69361 atccttactt ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg
    69421 agagaagatg ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct
    69481 tatttctatg cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc
    69541 gcagttgctg tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg
    69601 ttttacctcg cttttgtcgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta
    69661 gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa
    69721 caagatcctg gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg
    69781 ggagtgaaat ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc
    69841 gatccgaaag aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc
    69901 gctaaggaag atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat
    69961 tttgaagacg cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat
    70021 tacaaccaat tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat
    70081 atgattttta aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa
    70141 aatcagctag tcgtacagta ataggctagc tgagaataat cctgaaatat tagtagaaag
    70201 gagagtgaag ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt
    70261 cttgcttttg ttgctgacgc tagtcttttt tgtttggaca gcaggggtag gttttccagc
    70321 aactttgttt atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct
    70381 cattttaaat gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc
    70441 ccttttcatt ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt
    70501 actgtttcct ttggttattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg
    70561 ggatcgcggt atttatctga ttgtctttac ggtcttcctc ggaatcacgt tatatcgtgt
    70621 atttacagag atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc
    70681 tgatatttgg atcaatacga atacaatcgg aagttctttg atgactcttg ctttattgat
    70741 cagtggattt gccagttctt ttgaacgatg gtatgttcga atcatcggtg ggatagcagt
    70801 cgttgcagcc tttttaggta tttgggtctg ccaatctaga ggtgcattag tcgcgttgat
    70861 tgtttttgcg atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt
    70921 tattgcctac acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc
    70981 tgaaaaagtc aatttgttta ccgatcgaga agatatttgg cataaattct atcagacgct
    71041 aggagaaaaa tctgaacaag ttttactagg aatgaaaaca tttatcttcc agcgaggaaa
    71101 ccaattttta ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg
    71161 atttgggatt gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga
    71221 tttatccaac gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt
    71281 tatggaggat acattgactt catacccatg ggttccgatt atttatatcc tgttggctat
    71341 ggccgttcat cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc
    71401 atatgacgat atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc
    71461 ttccaggatc caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt
    71521 cgcagccttt tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga
    71581 gaaaatcttg tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat
    71641 atggtaaaat ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt
    71701 aaagatttgt atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt
    71761 tcgatcaatg acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt
    71821 atcaatccgc tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca
    71881 caaatgatcg atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg
    71941 ttcttcttct cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta
    72001 gtaaagaaag tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg
    72061 tacacccatt tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat
    72121 ccttcagcaa tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca
    72181 ggattgactt ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt
    72241 acgatcgtgc tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt
    72301 gattcaggtc ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat
    72361 cgtgagtcat ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc
    72421 tgggttttca cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca
    72481 catttttcag atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag
    72541 atatcacaaa atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca
    72601 atccttttaa gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa
    72661 taaaaaaagg tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt
    72721 tgaaaatcat tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa
    72781 tctcagcttt gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata
    72841 tctatttaaa tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg
    72901 atatcattga atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa
    72961 gcgggatgtt cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga
    73021 ttgtggatga agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat
    73081 ttacagaaat caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga
    73141 ttcgtcgttt ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg
    73201 atacgatgga agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag
    73261 tggatcggaa taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga
    73321 aagcggaatt gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac
    73381 tatacgttaa agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa
    73441 ttgcgctacg aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta
    73501 tcaagattcc ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt
    73561 tgttagctgg tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt
    73621 tggtatataa gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca
    73681 tcgtggtctt agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa
    73741 atggacctag atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt
    73801 aaagttttag agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa
    73861 caatgtgaag tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt
    73921 gctcaagatg gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga
    73981 cagacatttg attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa
    74041 acattgaaga aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct
    74101 aacttggcgc ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct
    74161 tatggtctcc tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt
    74221 tttgaaaaag cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat
    74281 acagaattaa aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg
    74341 cgaccatttg gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa
    74401 agttccattg cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa
    74461 acgaaacaag atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag
    74521 acactaagtt tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa
    74581 cctagcttta tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg
    74641 aatgctgtag tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag
    74701 ttcgttctaa cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc
    74761 ggggaattga caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc
    74821 acaaagcagc tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag
    74881 gaaaatacaa gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg
    74941 gaaagtgatg aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa
    75001 aaaatccaag aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca
    75061 aaaatattaa aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat
    75121 aagttatcag ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc
    75181 aatcaagcag aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc
    75241 gagcaagaac gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt
    75301 gaaatgtaaa taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag
    75361 aaattgccgt atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg
    75421 tgggctgggc aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa
    75481 atgtgattgc tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg
    75541 atgttaagac ccaaagcggt ttcaaggtac gtttatcagg gaaaatgaga ggaaaagcca
    75601 tcttagactt ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat
    75661 acccttatga tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag
    75721 ggataaatta tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat
    75781 tgaagccagg atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga
    75841 aagagcaaag gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc
    75901 ctgtctataa cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa
    75961 cgtatgatca ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa
    76021 agtgtctgga atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa
    76081 atggacacat cagtctggca actaattctg cattggaaat agcagaagga gaatttatag
    76141 cactcttaga taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc
    76201 taaacgtaca tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg
    76261 gcaatcgctt tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata
    76321 attatatctc tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc
    76381 gtaaaggcta tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc
    76441 ctgcagatca tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat
    76501 caactgcaag taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga
    76561 cagatgccct gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat
    76621 tttatgagat tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga
    76681 agaatggcta tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt
    76741 caaattatga aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat
    76801 ttgcagaata caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat
    76861 tcaattattc tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat
    76921 tcttgaataa cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg
    76981 cacagtttga tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc
    77041 agcatgcagg agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg
    77101 atcgaacaca ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca
    77161 ctgcagcttg tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa
    77221 cattagaagt agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt
    77281 acaatgtcta tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg
    77341 aagatactcc agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat
    77401 ggccagcata tattgcgcat gatccatttt acaatgataa tttaacaaaa gacggaatcg
    77461 gcgacttttc gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct
    77521 cgcacataac ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa
    77581 aaataagtcg aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa
    77641 aattgctttt caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc
    77701 accgtttatt cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct
    77761 tattcatgct cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc
    77821 tgtcaaagaa tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta
    77881 ttcctctaca aaagccactc tatcaattat catgggttta aaataagaag tgatggagat
    77941 aaccagattg ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata
    78001 tatttcgttc tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc
    78061 ttctaaagca ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg
    78121 ttttccttta tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc
    78181 cgataaacac aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt
    78241 ggttttagga gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata
    78301 attgacatgg tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt
    78361 tgttagctta cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata
    78421 gttgaattcg ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca
    78481 atcttcactt gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat
    78541 tgttagcaag tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata
    78601 ctctggaaaa taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc
    78661 aatgccaatc agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc
    78721 ttttttataa ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat
    78781 ttgacatata tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag
    78841 atattacctc actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg
    78901 attcttcacg taatacaggt attcttatgg actagacagc gctttgggaa gatggattct
    78961 ctgaaaaata taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt
    79021 ctttaattta ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct
    79081 tgtcttgatt agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt
    79141 gcttataaac taggttttta aaggtgacta tggtaagata atagagatat tattttgtga
    79201 ttaggagtgt atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta
    79261 tattaatgga tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc
    79321 gtggagagat cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag
    79381 tgatttttat tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca
    79441 gtgacatcgt gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag
    79501 tgacttttgc gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc
    79561 tcggtacgct tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa
    79621 gtcgagataa aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa
    79681 attttggttc aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt
    79741 atgcaaacat caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg
    79801 aagaaactga aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt
    79861 tgaatacaac atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg
    79921 aaagtattat tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt
    79981 tgtttgatat catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca
    80041 cagcaatttt agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca
    80101 caaaaaatca aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag
    80161 caaaatctgg accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat
    80221 ttattcgagc tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata
    80281 tgtcgatcgt aggaccacgt cctgaacgtt ctttcttcgt cgatcagttc aatgcagaaa
    80341 acccttatta ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct
    80401 atggaaaata tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa
    80461 aatattcatt gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata
    80521 aaatgtcctc tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt
    80581 ttccgaatga aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca
    80641 gcataatcgt accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa
    80701 aagaattgga ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa
    80761 cacaggaaat cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga
    80821 ccaatcaagg gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc
    80881 tcttattctt cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt
    80941 tgctagaaaa gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg
    81001 acgagggaag aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc
    81061 acgaagagtt tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat
    81121 ggaacaaatg ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata
    81181 atagttgtga ggatcgaata tttaacttac attattatga atactgtcat caagtgatca
    81241 tgaacccaaa agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat
    81301 accgacctaa caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag
    81361 acgatattaa taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt
    81421 tttctattta tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga
    81481 tattgagtga tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa
    81541 aaaaaataac aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag
    81601 ttggaagctt cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt
    81661 agttaatgga ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag
    81721 gtggagcaga gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat
    81781 ttgatttttt gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa
    81841 gattaggagg ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa
    81901 tagaagtgaa taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt
    81961 ttggcggggg aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc
    82021 atagccatgc aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat
    82081 ttttaaccaa attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg
    82141 gtgaaagtct gtttcaaaac catccctttg agatagttca taatggtatt gatttagagc
    82201 tttatagtaa aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga
    82261 gtacttttgt tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt
    82321 tagagatttt tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg
    82381 aagggtcact tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag
    82441 taaaattttt aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg
    82501 taatgccttc tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat
    82561 taaaattagt cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt
    82621 ttgtatcatt agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg
    82681 ctaataaaat aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaatgg
    82741 agagtatata tagtagagat cattaaaaat gttcaagata ttttaggagt gattattttt
    82801 gaaaatatgt tatttgatat tggcatacaa tgatattggc aacttaaaga gattggtcaa
    82861 tcgattgaac gaaaatgcag tttttattat tcatatagat aaaaaaactg atatagaacc
    82921 atttattgaa gcatttaaat catatgataa tgttgaattt ttatgtaata aaaaccgtgt
    82981 taaaatttat tggggtggtt tttccattgt acaagcagaa atcaatttag taaagagagc
    83041 attacaaaat gagaaatatt taaaatatgt attattatct ggtgctgatt atccaatcaa
    83101 agataatgag tatatttata attattttaa aaagaatagt tcagtggagt ttattcgtgg
    83161 aatagatcta gatcaaatta aacataaaga attttattat aaacatatag atgtatatca
    83221 aaaacatgat tatcctagaa ttaataggaa taacacaact gcatttaaaa tttttagagc
    83281 tataattaat cgttgtctga ggatgattaa acttccccca aaaattagac accataaatt
    83341 tgatttgtat catggttctc aatggtgggc tttgtcgaaa gaatgcctaa cagaattaat
    83401 acaaatgtat gaacaaaatc aacaagacta cctgaatttt aaaataggta tgtttgcccc
    83461 agatgaaaaa ttttttcata cacttttttt taattcctcg ttcaaaaata aaaatgttat
    83521 tggaggacca gatttaccac ttgaattgaa aaatattgaa gaaactactt tacaaacttc
    83581 gaaattagca aatattcata ttattgatcc ttccatgaac aaatggtttg atgaaaggga
    83641 tatagatcaa ataaaagaaa gtgaaaaatt atttgtaaga aaagttaaat cggattactc
    83701 tgataaacta ttaaatagaa ttgataaaga aattttgaaa gttggaaata tagatgaata
    83761 gaatagtacc tgttttagag aagagtttat taagtcgaga tgcgtttcga gtaataaatt
    83821 taatcatttg tctgctttat tttatgccta gtacttttaa tgtaactaat atcccgatga
    83881 aaatagcttt tgcttggggt ggaattatag ttttgtatga ggtttttata aagaaaaatt
    83941 tttttaaaat gcgtttttct tggctactga ttggagcaat gattagtttt atcttaacta
    84001 taatcattaa cagagaaaat tttttaattc catctattta taattttgga tatttgctga
    84061 ttactttgat agtaattttt cctgcagact tttcacaaag tgaagaggaa aaaaagaaaa
    84121 aaatggtgta ttttaataat gttttcatta tcataatttt tttagctgcg ctaatatcta
    84181 tctatcaatt tatttttttg atttcttatc atgtcccaac aggaacgcca ggattacttg
    84241 caagacaagg ttttattgaa catcggttat ttggtgtcta tacaagtcca aatgttggag
    84301 ctatgtttgg atatacttct attattttaa gtttaattag tgtattaatt tcaaaaataa
    84361 caaaaacgat tcgtgttttt tatatgatga attttatcgt acaaatattt tatattacac
    84421 tttcttcgtc tagaggtgcc caggtagtaa tttgttcttt tttagtatta tttcttttgt
    84481 tgtttgccaa ttcaaaattt agaaagagca ttagagaagt actacctgta aaaatcaaat
    84541 ggatggtaag catattatta gttctattgg tatatttttc ttttggtact aattatgcta
    84601 aggatttttt agcaacaatt cctgtatcga ttgatagaaa agtagaagtt atagagtcaa
    84661 atgaatcaga taaaactcca cttccagaaa aagaagtaat attacagcat tctagtgata
    84721 actccgaaat ttcagctggt cgttttacga tttggaaagc tgcgcttaaa gttataagac
    84781 caactttgtt atttggttat ggagagaccg atttctatgg gaatagcgat gcaaaatttt
    84841 tgaaaaaagt agatcttgat actacagatg tgaatgaact aaaaagagca catggaaaca
    84901 tgcataatgg atttcttcaa gtacttgtat caagtggtat aattgctttt atatgtatct
    84961 atgctttcta tgtgttaaat atctttagtt tactcaaaac attttttaga agtaaagtga
    85021 attatgcgtt tcttggtatt gttttaattt ttatactatc tatttttata gatgaaatgg
    85081 tggaggctca tgtacttttc aataagcgcg atgtcatttc aattgttttc tggtattatt
    85141 tgggaagcat ttcttacttg tatttaaaaa aggagaatta atatgaagac agctttcatt
    85201 gcttggactc ctttacattt gattaatata ttaaatgttt atcaaaattt ttaccgagaa
    85261 tcaaaaggag atttatttat ttattctgaa tttgaaaacg ctagaagata tagtgagcgt
    85321 ataaaagaat tagggctatt taatgatgtt gtttttgtga atcaccggaa aatcggttct
    85381 tcctttaaac gtaaaattaa cttgttaact aaccataatc aatttttctc ggatgattta
    85441 tccgtttatg atgaaatttt tatacagggg gaaaattatt tcacaaaaat acttttctcc
    85501 aatgtttaca aaaaaaatcc agatgtaaaa ctaaattata tagaagatgg tttgggtact
    85561 tatttgaaga agagtatatt ttcaacaagt aaaaacgttc aaagagtttt taaaatatta
    85621 aataaaaata gcatcttcaa aaaaaaaatt gacgattatt ttgtctattg tccggaattg
    85681 ttactttatg atgtaaaaaa tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg
    85741 atgaaaaaca tacaatcaat ctttgggata gaagaagttt ttgtcggaga taaatgtatc
    85801 ttttttgatc aacctttatt atctgatggt tatggcatag atgaaacaga tattttcgct
    85861 aaagtaaata gtatattttc tggaaaaaaa gaaactttaa ttaagcttca tcctagagca
    85921 aggacaaatc gttatggtga aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg
    85981 ctacttgaaa atgacttctc ttcaacactt ttgattagtc ctatatctac aatttctttt
    86041 tcaccatcta tgatgtttga aaagacgatc aactctatta tgttggctaa attaatttta
    86101 aatgaatata cagcattatc tgaagataaa aaagaaacat tgacaaatat tgttcttttc
    86161 tgtgaacatt ataaccgtct atcagaagaa aaaattttac tcccagaaaa ctgggcagaa
    86221 ttaagaaagt tggtagagta tgggattatt taacacactt tccaagtcaa aattagttaa
    86281 gtccagtttc tggtatacta tagggaattt ttttattaag ggaatttcat tcataactat
    86341 tcctattttt gtaaatatta ctactcaagc agaatatggt ttgattaata atttcacttc
    86401 atttgtatca attttttcga ttcttgttgg gctaagccta aattcggcaa ttaataatgc
    86461 aagttttgac tataaagatg acataaatga atttatgtca tcggtacttt ctctagctac
    86521 actttcgtta attgtgttct tattaatagg gaacatatat tttttgtttt caaatgcttt
    86581 ttttgatatg aaccaacttg tatttaattt gatgttatta caaagttttg ctagttttgt
    86641 aattagttat atccagtctt attttacgat taactttaac cattttaaat atttgatttt
    86701 atcttttata tctacttttt tgaatattgc aatctctgta gctttgatgc agacattata
    86761 ttttgatgat aaatatgttg gcagagtagt cggcggaaca ctgtcatttg tgttgatagg
    86821 tttagtaatt tatttaagaa taatgataaa gggtgctcat ttttttgata aaaaacagtg
    86881 gagatacgct ttaaaaatct ctttgccgct aatcccacat tccttagcaa atatagtttt
    86941 agcacaattc gatagaatta tgattaattc ttatttaggt cctgctgcgg ggggaatata
    87001 ttcctacatt agtaacatag gtattattct aagcgtaata tggatgtcta ctaataacgc
    87061 ctgggttcca tggttctatg gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt
    87121 tagtaactat tatttagtag tctttacttt gattacgatg attgtaatgg tagtttcgat
    87181 tgatttagca aaatttatgg ctccgaaaga ctattattct gggttatctt tagtggttcc
    87241 gataacctta ggctatttct ttcagttttt atatagttta ccagttaatg ctgaatttta
    87301 tgagaaaaaa acatcgttta ttgcgattgg tacagtagca agtgctatag ttaatatact
    87361 attaaacatg attttcatac ctaggttagg aataattgct gctggataca caaccgtatt
    87421 tacgtatttt ttacttttta tttttcatta taatatcgct aaaaaaatta cgaataaaca
    87481 attatttgac acaaaaaaaa tatgtatcat aataattttt gtatcaattt tttctttgat
    87541 acttcatatt ttaatcaatc aaatgttgat tcgttacgtt ttgacatcat tattgataat
    87601 tagtttagtt atatattttt taaagattaa gaatcgaatt atagatgact ataattcata
    87661 ggaggagata taagtgttta aaggaaaaac aatattagta actggcggaa ctggttccat
    87721 tggaagtgaa attgttcgtc agcttttaaa atatgaacca aaagctatca gaatttatag
    87781 tagaggtgaa gaaaaacaat tttatatgca acaagagctc tcaaatcaca ctaatttgag
    87841 atttttaatt ggtgatatca gagactctca aaggattgat tatgcatgtc gtgatgtgga
    87901 tattattttt catgctgccg caatgaaaca cgttccagca agtgaatata atcctatgga
    87961 agctgtaaaa acaaatgtga tgggaactca aaatgtaata gatgcagcga tcaaaaataa
    88021 tgtaaaacat tttgtaggta tcagtacaga taaagtagtg agtcctacta acaccatggg
    88081 tgcgacaaag ttattgagtg aaaaattaat aatagctgcc aatcagtata aaggaaaaac
    88141 agaaacaata ttttcttgtg taaggtttgg aaatgtcatg gggtcaagtg gatctgttat
    88201 tcctcttttt agtaaacaaa taaaaaacaa taaagatatt actttgacag atgttgatat
    88261 gacacgattt atgatgtcaa taccggatgc tgcaagatta gttctgtctg ctgctatgaa
    88321 ttcaaaattt ggagaaacat atgtcttaaa aatgccggca atcaatattt taactttagc
    88381 tcaatcatta ataaaaatat ataatgatac acatgatgaa aaatatagag gtgaaataaa
    88441 agttgtaggt atcaggccag gagaaaaatt atatgaagag ctaatgacta cagacgaaat
    88501 tgaacgctca tatgaaaatg ataaaatgta tatcattcca ccacactatc atgctactga
    88561 cgaactgtac aaagattatg aaaaaattaa aatcactgca tactcttcag atgcaacaga
    88621 attaatgagt ggacaggaat ttattagaat cgcaaaagag aataatgtcg tgacttggta
    88681 aggagtttta tgatgaaatc agaagtatat gtaattgcag aaattggtgt aaaccataat
    88741 ggaagtctag atttagcttt agaaagtatt gataaagcta aagaatgtgg agctaacgcg
    88801 gttaaatttc aaacatttaa aacgaataag ctagtttcca aaaaggctcc aatggcaaaa
    88861 tatcagaagg ataatcttaa agaggacagt tctcaatttg aaatgctaaa aaaattagaa
    88921 ttatctcaat ctgattttat tagaataaaa gattattgta tacaaaataa aattgatttt
    88981 ttgtctactc cttttgatga agaaagtgca gactttctaa aacaaattgg tgtacaagga
    89041 tttaaaattg gctcaggaga tttaacaaat ttaccttttc ttagaaaaat agatatgtat
    89101 gggttgccag ttttattatc tacaggtatg gctgatattc atgaggtcag tgaggctgta
    89161 gattgtttca tagatagtcc agttactatt ttacattgta cttcaaatta tccggctagt
    89221 tctgaagata ttaatttatt ggcgattaac acattaaaag aaatgttcgg gatacccgtt
    89281 gggtactccg atcattcatt gggatatgat gttggtattt gtgctgttgc gatgggtgct
    89341 aaggtaattg agaaacattt taccttggat aaggaactac ctggtccaga tcataaagct
    89401 tctttagatc ctcaggaatt tgcagatttt gtaaagcata ttagaaacac tgaaatattt
    89461 ttaggtgatg gtgttaagcg agcaatgcct tctgaactta gtacaaaaga agtatcaaga
    89521 aaaagcctag tggtcactga gaatttaaat gtgggagata ttcttacaga gaaatctctt
    89581 acaataaaac gtcctggaaa tggaatagct cctaagtttt tatatcaata tattggcaaa
    89641 aaagttaaaa gaccaataag tgcagatagt gttctgcttg aggaagatgt tgaatgaaaa
    89701 aaatacttat agtaagtgga acacgagctg attatggaat atataaacct gttgctgaga
    89761 atttactaca agataattgg gatgtatcat ttgttgtttc tggaatgcat ttatcccata
    89821 aatatggatt tactaaagaa ttgatcttag ctgacggatt tagaattagt ggagaagttc
    89881 aaagtttgtt tttagagaac aataagggaa acatggctcg gtctgtggca ttagagataa
    89941 atggtttcac aaatatattt gaacaaacta gccctgattt tgtattatta ctaggggata
    90001 gaggagaaat gttggcagct gcgatagctt gtttgtatct tggaatcaaa acagcacata
    90061 ttcacggagg cgaagtgtct ggtactgtag atgaatcaat aagacatgcc ataacgaaaa
    90121 tagcatctgt tcattttgct gctacggaaa aaagcaaaga gcgtttaata aaaatggggg
    90181 aagattcttg gagagttttt cccgtcggag ctcctagaat agatactatt ttaaataata
    90241 gattacctag ttttagagat ataaaagaaa aatatcatct aaaatttgaa aagaaaaaat
    90301 acgttttgca agtatttcat ccagttgtaa ccgaactaga aaatatagaa aaacaaattt
    90361 tagctttgat caattcaatg gaacaacaat ctcaaccaat tatttgcatt ttacctaata
    90421 gtgatgctgg tagcgaaatt atcagaaaaa tgtaccagaa atatttaaaa acggatatta
    90481 tttatattac gaatttagag ccggaggaat acctgactat attaaaagag tgcaaattta
    90541 tgatcggaaa ttcttcttct gggataattg aagctgcatc attcaaaata ccagtaataa
    90601 atttaggaac cagacaaaat ggacgagaac aatctaaaaa tattataaat gcaaatctaa
    90661 atgaaaatga aattgaaact gctttgaata aaatacaaga tcctcagttt ataaaaagct
    90721 tgaaaaatgt taagaacatc tacggtgacg gcaacagttc tagaagaata acaaaaacat
    90781 tgaatcaaat attgaatgat ggggaagata ttaagtggat tcaaaaacaa atagcttatt
    90841 aaaatatact agtgatccat acattattat tggggctggc ggacacgcaa aagtcattat
    90901 tgaaacctta agattagaaa ataagttaat atatggattt tttgatgata agcaaaataa
    90961 aatgcaagat ctgaaatatc ttgggaaaat aaacgaattg aatagctggg cggatagcaa
    91021 aaaaaaaatc tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat
    91081 aaaaaaattg aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt
    91141 ggcaaaagaa atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc
    91201 agatgctgta attggagagc atgctataat aaacacaggt agcattgttg agcatgattg
    91261 tagaattaat aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt
    91321 aggtgtagga actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc
    91381 ttggtgtatg ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc
    91441 ttatggcaat ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat
    91501 tatcaagtcc tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata
    91561 attggatagc gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata
    91621 ctggttctaa gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct
    91681 taagactgct taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag
    91741 ctacagtaaa tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta
    91801 atacatggaa tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca
    91861 accaattacc taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac
    91921 gaattttagc tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt
    91981 taggttctac ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat
    92041 ttaacggtaa taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa
    92101 aatatgttga gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc
    92161 aacatagcga aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg
    92221 gacaaatgga agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata
    92281 aggaagcttt ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc
    92341 caaatcgttg gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta
    92401 acgtaataaa taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac
    92461 atttgcaacc agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt
    92521 cagaaaagtt atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg
    92581 aacaagaatt tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga
    92641 acataggaat tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac
    92701 actttaatga tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg
    92761 aaaaggaatt tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa
    92821 taaattcagt ttttgtttgt acatccccta taacccatga atttattata aaagaggctt
    92881 taaatttaga ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa
    92941 taatcgaagt agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga
    93001 gaaaagaaat tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat
    93061 accatgtagg acagtattta cccgactggc atccttggga agattatcgc aatttttttg
    93121 tttcaaacaa aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta
    93181 tagatacatt tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg
    93241 atattgatta ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa
    93301 ctttaagtgt agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa
    93361 agattttaat agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt
    93421 ctagtatgac agatattcaa ttgtacgatg aggttatgca tgataataat tattcacata
    93481 ctattattga agatgcatat ttagaggaga ttaaagaatt tttcacgctt ttggaaaaga
    93541 aaatggaacc ccaatatact tttgaaaaag ataagtatat tattaactta attaacgaca
    93601 ttgaggtaaa ttaatgatga aagtaatttt ttttgggtta gggtcaattg gaaagagaca
    93661 tctaaaaaat ctaataagct atggaaaaaa gaattctatt aattttgata taacagctta
    93721 tagtagcagt ggaaaaaaag agtttgaaga aataacgtat attcaaaata gagacgattt
    93781 ggatacacat tatgatatag cttttattac aaatccgact tttaagcatc tggaaacact
    93841 aaatgtaatt gttgaaaaag ttgattacat atttttagaa aaacctgctt tttctgagag
    93901 tattgatctt actcaatata attatgatta caaaaatatt tatgttgctg ctccactgag
    93961 gtataaaaat atcatggaag ttgttcgtaa tagtattcaa agtaaaaaag tatattctgt
    94021 aagagctatt tgttctactt atcttcctga ttggagaaaa ggtactgatt atagaaataa
    94081 ttattctgcc tttaaatcta tggggggagg agtagaatta gactgtatac atgaattgga
    94141 ttatattata aacctatttg gtttccctaa atcagtaaaa aaacagtttg gtaaatattc
    94201 gaatcttgaa attaacagta atgatttagc aacatatata ttgagatacg atgataaaat
    94261 tatagaaatt catttagatt attttggaag agttcctaca agaaaatttc aattgtttac
    94321 tttagatgaa tgtatcaatg ttgacttatt aaataataaa ataagttttg aagaaaacat
    94381 tgttaaagaa atgcctgaat ctgctaatga tatgtatgag aaggagttga aatatttctt
    94441 tgaaaacgta gtaacaggca aagaaaactg gaatgattta tatcatgcaa acgaagtatt
    94501 gaaaatagct gaggagaaat aaatggaaat tttatttact atttgtggta gagctgggtc
    94561 taaggggttt aaaaataaaa atttgaaata tttggcagga aagcccctag tccatttcgc
    94621 tttagcaatg atagatatct atcaacaaaa acatcctgaa cataattgca cgacttgttt
    94681 gaatacggac agtaatgagt tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat
    94741 tgtagaacgt aaacctgaat tagctacaga tactgtaggg aaaattgaag tgatacgtga
    94801 ttcattctta caactagaaa gagaagataa tatatttgat ttaattatag atttagattt
    94861 gacttcacct ttacgtgaag tttctaatct tgaagaatta atagatgaac atctaaaaaa
    94921 tttagataaa gacgtctact tttcagttgt ttctgcaaga cgtaatccct attttaatat
    94981 ggtaaaaaga attgaaaatg aagttactat agttaataaa tcaagataca catctaggca
    95041 acagacacct gaagtttttg aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct
    95101 aaaaaaaagt aattatattt ttgatgggca atgtgggata ataaaaatga gagattatct
    95161 aattttagat attgatagtg aagaagattt taaatggtta gaatttttgt ttcctaaatt
    95221 tttagaagat tcttcggagc ttcaagatat ctataattat ttatatcgga ggtaaaagga
    95281 tgagctgtta tcttatcacc ggtggggctg gcttcattgg ttctacctta gccaatcatt
    95341 taggagaaga acatgtggta atcgtcgtcg atgacttatc aatgggaaaa gtagaaaatc
    95401 ttgatatgac gaaaaatatc acttttattg agggagatgt cgctgagaaa tccttgatgg
    95461 aagagatcat gaaagctaat aaatttgatt atatctttca tttagcagca gttgctagtg
    95521 tggcagattc agttgcccga ccagtagaaa cacatcgtgt gaattttgaa agtgtattaa
    95581 tgattttaga attgatccgc aagtatcaac ctgatttaaa acggatcgtg tttagttctt
    95641 cagcggctgt ttatggggat gaaccgactt taccaaaaaa agaagagtcc gtgattcgcc
    95701 cattgacgcc ttatgcggtt gataaattcg ctgcagaaca atatgtgcta gattattgtc
    95761 atttatatga tgtacccggt agcgcagttc gttttttcaa tgtctatggt ccaaatcaaa
    95821 atccgaattc tccatacagt ggtgtgattt ctattctcgt agatcgctat aagaaacaaa
    95881 tcgtaggaga agcaacttca tttacattat ttggtgatgg aagccagtct agagactttg
    95941 tttatataga tgatgtaatc caagcattac ttttagttgc gaatgaggaa gctgcactgg
    96001 ggaaacaatt caatgttgga acgggtaaag caaccacact tttggaattg attcatgcaa
    96061 tcaatgaaat tctcggtgta gaattgacac ttgaatacaa agaagagcgt tcgggtgata
    96121 tccatgactc tctagcagat attactaaga tccagtcaat tggctatcaa ccgaaatatg
    96181 gtattttaag tggaatgact aattatttga aaactgaaat caattaaaac atactaagat
    96241 aagattagag ggtaaattca ggctctttgt caataaggac tgatgagttt tgtaaaatca
    96301 aatctgggat agaa
//
LOCUS       DACECJ010000002        72311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.002, whole genome shotgun sequence.
ACCESSION   DACECJ010000002 DACECJ010000000
VERSION     DACECJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.002"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..940)
                     /locus_tag="IWO09_000098"
     CDS             complement(<1..940)
                     /locus_tag="IWO09_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262724.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEEST"
     gene            complement(1195..1704)
                     /gene="mreD"
                     /locus_tag="IWO09_000099"
     CDS             complement(1195..1704)
                     /gene="mreD"
                     /locus_tag="IWO09_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0262725.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1708..2562)
                     /gene="mreC"
                     /locus_tag="IWO09_000100"
     CDS             complement(1708..2562)
                     /gene="mreC"
                     /locus_tag="IWO09_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0262726.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2717..3355)
                     /locus_tag="IWO09_000101"
     CDS             complement(2717..3355)
                     /locus_tag="IWO09_000101"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0262727.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3356..4006)
                     /locus_tag="IWO09_000102"
     CDS             complement(3356..4006)
                     /locus_tag="IWO09_000102"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0262728.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4019..4918)
                     /gene="rsgA"
                     /locus_tag="IWO09_000103"
     CDS             complement(4019..4918)
                     /gene="rsgA"
                     /locus_tag="IWO09_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0262729.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5083..7152)
                     /gene="pknB"
                     /locus_tag="IWO09_000104"
     CDS             complement(5083..7152)
                     /gene="pknB"
                     /locus_tag="IWO09_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0262730.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7149..7889)
                     /locus_tag="IWO09_000105"
     CDS             complement(7149..7889)
                     /locus_tag="IWO09_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0262731.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7902..9260)
                     /gene="rsmB"
                     /locus_tag="IWO09_000106"
     CDS             complement(7902..9260)
                     /gene="rsmB"
                     /locus_tag="IWO09_000106"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0262732.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9260..10198)
                     /locus_tag="IWO09_000107"
     CDS             complement(9260..10198)
                     /locus_tag="IWO09_000107"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0262733.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10191..10682)
                     /gene="def"
                     /locus_tag="IWO09_000108"
     CDS             complement(10191..10682)
                     /gene="def"
                     /locus_tag="IWO09_000108"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0262734.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10697..13105)
                     /gene="priA"
                     /locus_tag="IWO09_000109"
     CDS             complement(10697..13105)
                     /gene="priA"
                     /locus_tag="IWO09_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0262735.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13358..13663)
                     /locus_tag="IWO09_000110"
     CDS             complement(13358..13663)
                     /locus_tag="IWO09_000110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0262736.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13668..14282)
                     /gene="gmk"
                     /locus_tag="IWO09_000111"
     CDS             complement(13668..14282)
                     /gene="gmk"
                     /locus_tag="IWO09_000111"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0262737.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14769..15053)
                     /locus_tag="IWO09_000112"
     CDS             complement(14769..15053)
                     /locus_tag="IWO09_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262738.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15071..15961)
                     /locus_tag="IWO09_000113"
     CDS             complement(15071..15961)
                     /locus_tag="IWO09_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0262739.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(15981..16199)
                     /locus_tag="IWO09_000114"
     CDS             complement(15981..16199)
                     /locus_tag="IWO09_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262740.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16169..17869)
                     /locus_tag="IWO09_000115"
     CDS             complement(16169..17869)
                     /locus_tag="IWO09_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0262741.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18136..18909
                     /locus_tag="IWO09_000116"
     CDS             18136..18909
                     /locus_tag="IWO09_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262742.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(18990..19559)
                     /gene="thiT"
                     /locus_tag="IWO09_000117"
     CDS             complement(18990..19559)
                     /gene="thiT"
                     /locus_tag="IWO09_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0262743.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19625..19727)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19733..20704)
                     /locus_tag="IWO09_000118"
     CDS             complement(19733..20704)
                     /locus_tag="IWO09_000118"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0262744.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20798..22171)
                     /gene="glmU"
                     /locus_tag="IWO09_000119"
     CDS             complement(20798..22171)
                     /gene="glmU"
                     /locus_tag="IWO09_000119"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0262745.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22674..23492)
                     /gene="purR"
                     /locus_tag="IWO09_000120"
     CDS             complement(22674..23492)
                     /gene="purR"
                     /locus_tag="IWO09_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0262746.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23659..24483)
                     /locus_tag="IWO09_000121"
     CDS             complement(23659..24483)
                     /locus_tag="IWO09_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0262747.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24456..25136)
                     /locus_tag="IWO09_000122"
     CDS             complement(24456..25136)
                     /locus_tag="IWO09_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0262748.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25159..26097)
                     /locus_tag="IWO09_000123"
     CDS             complement(25159..26097)
                     /locus_tag="IWO09_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0262749.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26342..27193)
                     /locus_tag="IWO09_000124"
     CDS             complement(26342..27193)
                     /locus_tag="IWO09_000124"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0262750.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27347..29920)
                     /gene="mprF"
                     /locus_tag="IWO09_000125"
     CDS             complement(27347..29920)
                     /gene="mprF"
                     /locus_tag="IWO09_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0262751.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30061..30867
                     /gene="proC"
                     /locus_tag="IWO09_000126"
     CDS             30061..30867
                     /gene="proC"
                     /locus_tag="IWO09_000126"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0262752.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30946..32502)
                     /gene="rny"
                     /locus_tag="IWO09_000127"
     CDS             complement(30946..32502)
                     /gene="rny"
                     /locus_tag="IWO09_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0262753.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32913..33962)
                     /gene="recA"
                     /locus_tag="IWO09_000128"
     CDS             complement(32913..33962)
                     /gene="recA"
                     /locus_tag="IWO09_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0262754.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34071..35312)
                     /locus_tag="IWO09_000129"
     CDS             complement(34071..35312)
                     /locus_tag="IWO09_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0262755.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35552..36130)
                     /gene="pgsA"
                     /locus_tag="IWO09_000130"
     CDS             complement(35552..36130)
                     /gene="pgsA"
                     /locus_tag="IWO09_000130"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0262756.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36339..37223)
                     /locus_tag="IWO09_000131"
     CDS             complement(36339..37223)
                     /locus_tag="IWO09_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0262757.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(38001..39287)
                     /locus_tag="IWO09_000132"
     CDS             complement(38001..39287)
                     /locus_tag="IWO09_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0262758.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39280..40548)
                     /locus_tag="IWO09_000133"
     CDS             complement(39280..40548)
                     /locus_tag="IWO09_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0262759.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40800..41261
                     /gene="mscL"
                     /locus_tag="IWO09_000134"
     CDS             40800..41261
                     /gene="mscL"
                     /locus_tag="IWO09_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0262760.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41321..41767)
                     /locus_tag="IWO09_000135"
     CDS             complement(41321..41767)
                     /locus_tag="IWO09_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0262761.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41860..42105)
                     /locus_tag="IWO09_000136"
     CDS             complement(41860..42105)
                     /locus_tag="IWO09_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262762.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42221..42799)
                     /locus_tag="IWO09_000137"
     CDS             complement(42221..42799)
                     /locus_tag="IWO09_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0262763.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42876..43406)
                     /locus_tag="IWO09_000138"
     CDS             complement(42876..43406)
                     /locus_tag="IWO09_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0262764.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43410..44270)
                     /gene="rlmB"
                     /locus_tag="IWO09_000139"
     CDS             complement(43410..44270)
                     /gene="rlmB"
                     /locus_tag="IWO09_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0262765.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44263..44658)
                     /locus_tag="IWO09_000140"
     CDS             complement(44263..44658)
                     /locus_tag="IWO09_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0262766.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44655..46067)
                     /locus_tag="IWO09_000141"
     CDS             complement(44655..46067)
                     /locus_tag="IWO09_000141"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0262767.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46064..46600)
                     /gene="cysE"
                     /locus_tag="IWO09_000142"
     CDS             complement(46064..46600)
                     /gene="cysE"
                     /locus_tag="IWO09_000142"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0262768.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(46922..48382)
                     /locus_tag="IWO09_000143"
     CDS             complement(46922..48382)
                     /locus_tag="IWO09_000143"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0262769.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48552..49709)
                     /locus_tag="IWO09_000144"
     CDS             complement(48552..49709)
                     /locus_tag="IWO09_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0262770.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49743..51113)
                     /gene="radA"
                     /locus_tag="IWO09_000145"
     CDS             complement(49743..51113)
                     /gene="radA"
                     /locus_tag="IWO09_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0262771.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51176..51703)
                     /locus_tag="IWO09_000146"
     CDS             complement(51176..51703)
                     /locus_tag="IWO09_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0262772.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51933..52739
                     /gene="proB"
                     /locus_tag="IWO09_000147"
     CDS             51933..52739
                     /gene="proB"
                     /locus_tag="IWO09_000147"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0262773.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52736..53983
                     /locus_tag="IWO09_000148"
     CDS             52736..53983
                     /locus_tag="IWO09_000148"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0262774.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54059..54403
                     /locus_tag="IWO09_000149"
     CDS             54059..54403
                     /locus_tag="IWO09_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262775.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54520..54846)
                     /locus_tag="IWO09_000150"
     CDS             complement(54520..54846)
                     /locus_tag="IWO09_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262776.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55461..56663)
                     /locus_tag="IWO09_000151"
     CDS             complement(55461..56663)
                     /locus_tag="IWO09_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0262777.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56780..57295
                     /locus_tag="IWO09_000152"
     CDS             56780..57295
                     /locus_tag="IWO09_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0262778.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57494..57940)
                     /locus_tag="IWO09_000153"
     CDS             complement(57494..57940)
                     /locus_tag="IWO09_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0262779.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(57987..59465)
                     /gene="msr"
                     /locus_tag="IWO09_000154"
     CDS             complement(57987..59465)
                     /gene="msr"
                     /locus_tag="IWO09_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0262780.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59819..60448)
                     /locus_tag="IWO09_000155"
     CDS             complement(59819..60448)
                     /locus_tag="IWO09_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0262781.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60432..60896)
                     /locus_tag="IWO09_000156"
     CDS             complement(60432..60896)
                     /locus_tag="IWO09_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0262782.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(61383..62651)
                     /locus_tag="IWO09_000157"
     CDS             complement(61383..62651)
                     /locus_tag="IWO09_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0262783.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(62882..63619)
                     /locus_tag="IWO09_000158"
     CDS             complement(62882..63619)
                     /locus_tag="IWO09_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0262784.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(63686..64255)
                     /locus_tag="IWO09_000159"
     CDS             complement(63686..64255)
                     /locus_tag="IWO09_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0262785.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            64383..65255
                     /locus_tag="IWO09_000160"
     CDS             64383..65255
                     /locus_tag="IWO09_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0262786.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            65597..66469
                     /locus_tag="IWO09_000161"
     CDS             65597..66469
                     /locus_tag="IWO09_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0262787.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            66466..68424
                     /locus_tag="IWO09_000162"
     CDS             66466..68424
                     /locus_tag="IWO09_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0262788.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(68756..69607)
                     /locus_tag="IWO09_000163"
     CDS             complement(68756..69607)
                     /locus_tag="IWO09_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0262789.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            69730..70197
                     /locus_tag="IWO09_000164"
     CDS             69730..70197
                     /locus_tag="IWO09_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0262790.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(70460..70942)
                     /locus_tag="IWO09_000165"
     CDS             complement(70460..70942)
                     /locus_tag="IWO09_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0262791.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71042..71905)
                     /locus_tag="IWO09_000166"
     CDS             complement(71042..71905)
                     /locus_tag="IWO09_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0262792.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        20269 a        15494 c        12938 g        23610 t
ORIGIN      
        1 ctgttgattc ttctgttgaa gattcaggtg ttgttgattc ttctgttgct gaagattgtg
       61 tagctgatga tgattcttca gtcattgaag attgtgtagc tgatgattct tctgttgctg
      121 agctttgtgc agtagatgaa gcttgagtag cttgtgcttc agcctctgct tgttcgcgtg
      181 cttctttttc agctttttct tgtgcagctt gttgacgagc ttgttctgct agacgttgtt
      241 gttcacgaat acgagcttgc tctgcttcag cttctgcttt ttgacggttc aagtcagctt
      301 tcttgtcttc agcagttgct tgttcagcag ctaatgaagt tttcagtacg ttcaaatcag
      361 cttgtttaga taaaagatcg cctttttgag attccaaagc agcttggttt tcagctaatt
      421 cttttagttt agcatcgttt tcagcttttt tgtcttctac agctttttta tcttgttttt
      481 gttgttccat caagtcgttg tttgctttta ccatcgttgt cattgcttga acgcgtccaa
      541 tagcatctgc taatgaatca gcattcaata cagcatcgat gtagtttgag cttgtgttgc
      601 ttacttgtgc ttcgcgtgct tgtttttgga ttgtatcttc acgtttttca atacgttctt
      661 gtaaatctgc aatgtctttt actaattgtg cagattcttg acgcaatgta tcttgttttg
      721 ctaataggtc ttgtgcttgt gtgttgattt cagaaacttg gctttctaag gcatcgattt
      781 gtgattgtgc atctgcttgt tgatttttca agtctgcaat cttttgatct tgctgttgta
      841 tctgagaatc aaaatcgtcc gccgcggcag cgatcggtga tgctacggca gtcaaggtca
      901 ttgaacatac cattactgct gatattaaac tctttttcac tcttcattcc tccgactggc
      961 ttaatttaat aaatatctaa atatatttaa gtctttctta acgatataaa aattgtacca
     1021 tagcagtgtg tcaacgatat aatagttatg ttacaaaaat gtttcaaaac aacttttaaa
     1081 aatgacaaaa ctgttcgttt cagaaaatga tcatgaaaaa gccgcgttaa gtctagcatt
     1141 tcttaagata acaacgattc tttttcgctt tcgacctaaa aataaaaaaa gaaattattc
     1201 atcactgaat aatttcttaa agggaaaaat gaataataca aatataatca tgttcaacag
     1261 cagtgtaggt cctaaaaacc ttgtaataaa atacgtgtta tttactactg ctaatttaaa
     1321 aatcaactgg accaccatcg taaacaattc ataacccgtg acaaagatga tcatactgaa
     1381 aaattcagta aagatgttga catggataac gtctttcatt acatacatca accatacaat
     1441 taaagggaga gctactgcat agataccgat aacgccaata tagtaagcgt catagatagc
     1501 cccgagaaca atcgtggtaa tcagtaaata acgtttttca aacgccatac tgcaacataa
     1561 taatgccaat atcagaaagt gggcatttga catataggtg cctttcgacc actcccctag
     1621 cattcttgtt aaatgaccat ctatcaacat caaaaagaac aaaacgattg gcagatagta
     1681 cttcattgtt tctttcttca acatggacta atcctccgct agccgttgga caatcgtcac
     1741 aactgacacg tcatacattt ctgcataagg tttcacataa acttcccgat ccagcccata
     1801 actatctggt ttcgttttaa taaccgtacc gatcggcaag ttagctggag aattccctcc
     1861 aagaccggat gtctggacaa catccccttc tttgatatcc gtatcaccag ttaattgggt
     1921 caccactaag gcatggagct tttcatcata gtttttaagc aaaccaaacg cttcgccatt
     1981 agccgaagat acccgtactg gaaaatgatt ggagctttca ttagaggatg acagtaattc
     2041 gattttagac gaagccgtat tgacctcgat cactcggcca atcaatcctt tttgcgacat
     2101 gactgccata tttgcttcga taccgtcttt agaacccttg tctacgatca gcatgtcctg
     2161 ccaactgtca ggcgaacgag taatgacatt tgccgttact ttttcgtaac tagtcagcgt
     2221 ctgattcaac tcaagttctt ttttcaacgc ttcgatttct ctctcgtaat tcttgttttg
     2281 ctgtgaaacg tcatcatagc tatcgatttt ttcttttagc cgctcatttt cggcataagt
     2341 tgaaaaaaga ttatggacag aatctactcc atttcgtacc caccgtactg gggcagagat
     2401 tgttttatca accattgcaa cactgtcatt aacgatggat tggaagaaat tgggctttcc
     2461 ttcattcgct cgctgcgcag cggtcacact tagtatcgtc accacgatga taacaataac
     2521 caaggtaata ataatatttt tattaggatt gaacttcttc acaaagacac ctcgaattaa
     2581 ttagactctt gtgttcaatt ttatcactaa atgccgtgaa acaaaaggta agttgctcct
     2641 cttaagcttt tttttagcaa ataatagatt ctgtcatttt tagcctgaaa gacaaatttt
     2701 tttatttgtt tatggatcaa tctttgcttt gaataactgc aatcatacca tctgaaaaag
     2761 aaaattctgc ggtatctgaa agaaattcgt tacttgcata agatgtcgga tattctactt
     2821 ctttattttc caatacatac ttgctgcctt ttaatgtaag attcgagacg ggagtcaagc
     2881 agcaataagc taaatacttc atatctggta tctgtcttac tatatgctct ccaggcaaat
     2941 aataagacaa ataattttga caatctctga taataagctg atgggcaaag ggtttgaagc
     3001 gaggttccat acctaaccag agattggcta aaagatgatc caatcttccg cccgtagctc
     3061 caatcaaagt gacttccgct tgcgggtaac gttcaaatgt ttttaagata cctaactgtg
     3121 tatctgtatc gtctttttct gcaggggaac gggtatactc tttggctttt gcaaatactt
     3181 ccttctgctc ttcgttcgac aatgaatcaa aatcacccac tgccagatct actgggtagc
     3241 ctgcttgaag caggtacagc gcaccacggt ctatcccgac ataggcatcg tatttggaca
     3301 gcagagactg cggccagttt tgcggattac ctccagcagc taataaaatt ttcatttagt
     3361 ctaatgcctc ttttaatcgt tcgatttgtc cgactggatt ttcggctcca tagatatatg
     3421 atcctgcaac gaagacatcc gcccctgcat ctttacagat ttttgctgtt tctggaacga
     3481 ttccgccatc tacttcgatt gcataagtcc aattgttctc tctacgtaga tcagcgagtt
     3541 ccgtaatttt ttctactgtt tcttcaataa agctttgtcc gccgaaccca ggattgacag
     3601 tcatgactag tacttgatca gccaaaggca aaacatgttt gatcatggag actggtgttc
     3661 ctggattgat aactactcca gctttaactc ctaagctttt tgtcatttga agtgcccgat
     3721 gaatatgagg agttgcttcg acgtggacag taatgatatc tgctccagct tttgcaaatg
     3781 ctggaatgta gttttctgga tcaacgatca tcagatggac gtctaacggt aatttagtaa
     3841 caggacggat tgcctgaaca atatttggcc ctaatgtgat atttgggaca aatcggccat
     3901 ccattacatc tacgtggatg taatctgcgc ctcctttttc cactaactca atgtctcttt
     3961 gtaaattggc aaaatctgca ctcaaaatgg atggtgctat cttcattctt gtttcctcct
     4021 attttcgttt accttctttt tttgcataca ttggttttct tttttcgatt tcttgtaaga
     4081 attgcaaata attgtcgtaa cgtgtctgtg cgataccacc tgtttctact ctatgtttta
     4141 cttcgcagcc aggttcttta gcatgcatgc actctctaaa tttgcaatgg acagaagctt
     4201 ctacaaattc aggaaattgt ctcggcagtt ctcctgcttc aattgtcaaa aagtcaatcg
     4261 agctaaatcc tggtgtatct gctactaacc cgccatagat aggaatcagt tccacatgac
     4321 gtgttgtatg ccgtcctctt ccaagtgcat cagaaatctc tcctgttgct aaattcagtt
     4381 ccggtgatat ttgattcagt agcgtcgatt ttcctgctcc agactgaccc ataaagaccg
     4441 atagccgttc tgggaagtaa cggatcagtt ctgtcgtatc acccgtatga ttgggtacaa
     4501 tcaccggata gcctatttta gtataggttt gcttgatctc ttcgattttt tctgggtcgt
     4561 ctgacaagtc ggtctttgtc aaaaaaataa tcggttcgat cgactcgtat tccaaggtaa
     4621 ctagaaagcg gtcaagtaga ttataagaaa atgcaggctc tacgatactt gtgacaatga
     4681 ttccaagatc aacatttgtg actggtgggc gaactaattg attttttctc ggcaagattt
     4741 ccagaagata gccgtctgtt tgattttcac tctcaaagat cacctgatct ccgacaagcg
     4801 gcgtgatttt ccgattccgg aaatttcctc ttcctcttgt ttgataggtc tctccgtttg
     4861 cgtatacata gtaaaaaccg ctgattgctt ttctgatttg tcctttgata agtgccacct
     4921 ccgtttttgt ttattttacc atacgaagag gaaagtaggt gttattccat gcaaacttct
     4981 caaagtatat ggcttgaaaa ttgatcaaaa gagatagaaa cacaaaaaag agaatgcaac
     5041 aaatcaatgt cgcattccct tctgatatat tttattcagc acttattttg aactttctga
     5101 ggtgctagct gatgaacttg tggagctgga agattcttct tttgaagagt tgcttgtgct
     5161 actggagcta cttgagctgc tagaactgtc tgaagagtct ttttcagaac tagaagacgc
     5221 tggatctggt cctttagagt aaattacgtt cacaaccatt cctttggtaa ttgtcgttcc
     5281 tgcccctggt actgtacgaa tcactttatc tttatccacc gtgtttgaat attcggaagt
     5341 ctcatgcccg atatattctg caccgacact agctaaataa ctttgtgctt cgctttttgt
     5401 atagccggag atatcattca atttaggctc tgttccttgg cttacgacaa aattgatgta
     5461 agtttcttct gctacaactt cacccgaggc tggatcctga ctgatgatcg aacctgatgg
     5521 tacagtgtcg ctatagtcat aagtagcact aatattattg atattgaatc cttgtgtaac
     5581 aagccaatta cgaacagttt cgtagttttc tcctacatga ctactcaaag tcactgtttc
     5641 tggtcctttg ctgactgtga aagtaatcgt gtctgtttta ggatcgactt ctgtatcttc
     5701 tgatggagat tgcgaaatga tcattccgct ttctacctct gaactgaatt cctcttcttt
     5761 ttgaatcaag tcagcagaaa accctaattc tttcaaactt gcttcggcat cttcataact
     5821 tttaccactg taatcatcta atttgatttt tttcgtgccg ctactaatat acagagtgac
     5881 ttcgcgtttt tgcttgactg tcgttcccgc tgccgggtct gtcttcacta cattaccttc
     5941 ttcgattttg tcatctgcaa tttcttctgt ttttgttgct acttctaatt tcgcttttgc
     6001 gagtgtttct ctagcagaag cttcagaaag atcagaaaca tcaggaacaa caacttctcc
     6061 tcgcccgcca gaagcaaaga atacagctgt tccgattcct aaaacagcaa gaatagctaa
     6121 taggatcagc caccattttt tctgtccttt ctttttagga tcaggcagaa cttcttcctc
     6181 ctctggtaag gtttcgtttt ctggcttgtc ttttggcaga ggcatagatt tgaatgaatc
     6241 aggcattggt gcatcttccg taaggggtgt gataactttt gtttcattat ccatcacatg
     6301 aggctgccat ttttcttcgt ttgcacgagc tggtgataat actgtagaaa gatcatcaga
     6361 catttcctcg gctgacttat aacggtctgc aggttcttta gccgttgccc gtaaaatcac
     6421 gttttccagt gcttgtggca catttggatc aagtgctttt atagatggaa gatcatcttg
     6481 aaaatgtttt aaagcaattg ttacagcaga ttcaccatca aacggtacgc tacccgtcaa
     6541 catctcataa aggatgatcc ctaaagcata aatatctgac tgcttcgttg ccatacttcc
     6601 tctggcctgc tcaggggaca gataatgaac agatcctagc atcgtattcg tctgcgtgat
     6661 cgatgtttca gataaagcga tagcaatccc gaaatccgtg atttttacaa caccttcatt
     6721 atcgatcagt acgttttggg gtttcaaatc tcggtggatg atttgatggt tatgagctaa
     6781 agaaatagca gaaaggatct gctgcataat atttactgct gtttcataag gaacaggata
     6841 atgtgtttgg atatatcgtt tcaagtccat tcctttgacg tattccatca ccagatactg
     6901 catgttgtct tcttcaccaa catcgtaaac actaacaatg tttggatgaa cgagttctgt
     6961 tgctgctaaa gcttctctct gaaatcgtcg aatcgcagtc tggtcatttt gaaagtcaaa
     7021 tcgtaagact ttaactgcta catcacgatc caagattaaa tcgtgcgcca aaaatacatt
     7081 tgccatacca ccgctaccga tatttccagt gatttgataa cggccattta attttttgcc
     7141 caactcgatc atacactgtc ctccttatat tcgataagaa gaacggtgat attatccgct
     7201 ccgcctgctt cattggcacg atcaatcagc tcagttactt tatgagacaa tgggccttct
     7261 tgattgatga tcgttcctat ttcttcttca gacagcatgt tcgtcaagcc atctgaacat
     7321 aacagcagtc gatcttttag ttgccaaaga tgagtagaaa catctacttc gactgttcca
     7381 ggcatgccga cagaacgtgt taaaatattt ttccttggat ggttcactgc catttcttcg
     7441 ctgatttctc ctgatttgac tagttcattg actaaagaat gatcctccgt caattgcatg
     7501 attttatctt cacgaatcaa ataagctctg ctatccccaa cattggcaat cgtgaaccag
     7561 tcatctgaca gagcggccgc tacgattgtt gttcccatac cgttatattc cggctgttct
     7621 tgtccacgct gataaatcag catattttct tcttgaatcg attgaatgaa ccattgggcg
     7681 attttttcat cgtcaacagc ttcttgtttt tcccaagctt cgcctagatt cgttaccgcc
     7741 atctgactgg caatgtctcc ggcacgatga ccacccatcc cgtctgctaa tacagcaagc
     7801 ttgagacctg aacggctggt aaatacactg gcatagtctt gatttgtgtt acgtcgtctt
     7861 cctacatctg attgatattc aatctgcatt ttttcacctc attacttttt gcgcaaacaa
     7921 caaatgaaaa atccatctgt gtaaaactga tgcggataca aagtcaacat tttctcttcg
     7981 attgatgatt gtagcatagg attggtcaaa acatcaatcc tctcaaaatc agggtgtctt
     8041 tccaagaaag cagaaacaac gtcttggttt tcttctttta agatcgtaca agtgctataa
     8101 gtcagaatac cggaagactt taaagttggt gcgcagcttt ctaaaatttc caattggatt
     8161 tttggtaaat tcgctaattc atccggctgt tttttataac gaatatctgg ttttcgccga
     8221 agcagcccaa gacctgaaca aggcgcgtct actaaaatcc gatcaaactg ctctgcttcg
     8281 aactgtttac tgacttctct agcatccatc ttttgagcgt agacagcaga agagacacct
     8341 agacgtgctg cattatcttc aatgagtttg actttgtgtt cgtgtacatc taaagcgacc
     8401 acttctccac cgcatgacgg atctaaaaaa gaagcaatat gcgtcgtctt acctccgggt
     8461 gctgcacagg catctaagac atgatgttca ggctcgattt gcatagacgg cgctacaagc
     8521 atcgaacttt catcttggat cgtcatgacg ccatcgtgga acaatgagct agaagctaaa
     8581 tgcccttttt tcgctactat accgtatgga ctgacttggc ttttttcagc tgtgatctct
     8641 tcttcttgca aaacagtgat ggcctgatct ctggaaagtc ggcgtgtatc tactcgggca
     8701 ctagcatggc taggctgata tagcgataaa ccgagctttt cagtttcttc gtatccgatt
     8761 tgatccacca gtcgtttcgt catccataga ggcaggctga ttttgacaga aagtctttcg
     8821 ataggatcag caatgccttg cagatctggt gctccttgac gctgataatt cctcaagaca
     8881 ccatttacaa atttccctgt cccaggattt ccttttactt ttgcgatttc gaccgcctcg
     8941 tttaaaatag catgtgcagg gactttatct aaaaattcta attggtatac agaaagataa
     9001 agaagcatct tgacccagtc atccactttt tttgctttag cgatcaatgg tgcaacgtaa
     9061 aattccaata gtcgctgatg gctaatcgta ccatagatca attctgtaaa taaacccaca
     9121 tctttttgat ttagctcgct ttgtttgatc atctcattca acaaaagatt cgagtaggcg
     9181 ccacccttgt tcactcgctc taatgtcagt aatgccatat atcgtactga ttttttcatt
     9241 tttttaggga tttttttcat tatttcacct gctgaccaat ctgaacttgc tgaccgcttc
     9301 cgttaaggaa atcatgtacg gcctgttttc ctttaccagc tggctgtaat tcattgatgg
     9361 ctaagacggt ctgtttaccg caagcaatcc atagattttt tttgtctttc cggatgatcg
     9421 taccaggtgc tttgtctgta gtttctgcca atacttcaac cgataataat ttccagcgta
     9481 tgccctcata ggtcgtgaat gcaattggcc aaggacgcat tccgcgtact tggttgtcaa
     9541 tttcttctgc agttttatgc caatcgattg cttcttgttc ccgtgtgatg tttggcgaaa
     9601 aagttgcttc tgactcgtct tgaggtactg gtttttgtcc atccaaaata ttgggcaatg
     9661 tttctagcaa tagctttctt cctaaaaggc taagcttttc aaacatcgtc cctacatcat
     9721 cttgtttagt gattggtatg ctttcttgcg catagattcc gcctgcatcc atttttttga
     9781 tcatttccat gatcgttaca ccagtttctt tctccccatt catgatggca taatgaacag
     9841 gcgcacctcc ccgatattta ggaagtaaag aagcatggac attgattgcc cctaattttg
     9901 gtacttgcaa taacttttca ggcaagaatt ggccaaatgc cgcagtgacg atcacatcag
     9961 gcaccaattc aatgatttct tccatttctg gcgaaccgga gattttctct ggctgcagca
    10021 cacggatacc gtgcttcaga gccgcttttt ttaccggtgt cggcgtaatt actcgtttcc
    10081 gcccaactgg acggtcgggc tgtgttacca ctgcctggat ctcgtaacct gattctatca
    10141 gcccttcaag aatcggcaca gaaaaagcag gtgtgcccat aaatactagc ttagtcatca
    10201 agatgttcct ccatatattc ttcaagttct tcttccggga tctgctgtat cattttctca
    10261 atgaacaaga taccgtctaa gtgatcgatt tcatgctgga aggcacgggc taaatagcca
    10321 aaagccgtca cttctatctc ttctccgtct cgatcataat aacgcacggt cacttcatct
    10381 gctcgcttga ccgttccata gacatgcgga atactgagac aaccttctac gtcaaggctt
    10441 tcaccttttg cttcgatgat ttctgggtta atcagttcaa atttctcgcc ttcatctact
    10501 tcgatcacag caattctctt gttttgtcct acttgaggag cagcgatccc tattccgtca
    10561 ttcgcaatca ttgtctcata taaattgtct aataaagcga ttgtctcgtc cgtgattaca
    10621 tcgattggct gagcagttct ttttaattta tcattagggt ggatcaatat aggatagcgc
    10681 ataatttggc cctttcttaa ataaagttta atggttcgtt gtcaattgcc acaaaaagac
    10741 cttgtcgctg ttctttctgg cttccattca aaatttcatc caaaacatga ggaagattcg
    10801 gttcatgttt atatttgatg attagttgat aataatatcg gtttttcact ctcaaaatca
    10861 tgctgggcgt tggccctaat agcaagcttt gagggcttaa cacttctttt agctgattgg
    10921 caatctgaaa gattttcttt gccgctactt gttcttcagg atggctggcg gttattttca
    10981 cagtaaagta ataaggagga tagcctccac gatgccgtac atgcatttcc tgctgataaa
    11041 actgctcata attttgtttt ttagccaatt ctatggcgta atgttgagga ttgaatgttt
    11101 ggatcacgac ttcgcctgct ttttccgctc gccctgctcg tcctgaaact tgggtaagca
    11161 attggaatgt atgctcacta gaacgaaaat ccggtaaatt cagggaggta tcagcattca
    11221 acaccccaac taatgtcact tctggaaagt ccaaaccttt agcaatcatt tgtgtcccta
    11281 ataatatatc tgcttctttt gcaccgaatt tttgtaaaat ctgttcgtgt gccccttttc
    11341 ttcgagtggt atctacatcc atacgcaaaa tcctagcttg cggataaagt tctcggagtt
    11401 cctcttctac tttttgagta cctgttccat agtagcggat tttattgccg ccacaattag
    11461 gacagcgatt gggaataggt tcttcatgtc cgcaataatg gcagcgcatt gaacgtgtat
    11521 ccatatgcag tgtaagagag atatcgcaat taggacatgg aagaacaaat ccacagtccc
    11581 tacacatcac aaaagacgaa tatcctcgac gattgagcag taggacagtt tgttcttttt
    11641 tgtctaatcg attttgtatt ttttcctgca aattagcaga aaaagtagag gtccgatgat
    11701 tctgaaactc ttcacgcata tctacgatct caatagctgg aagctgtgca gctgctttgg
    11761 ctcggtggtt taactgcaat aactgataaa cgcctttttg agctcgtgct cttgattcta
    11821 aagagggcgt cgcgcttcct agaactattg ggcaatgatg atatttactg cgccaaattg
    11881 ctagatctct cgcatgataa cgcggtgtct catcttgttt gtaacttgat tcatgttctt
    11941 catcgataat gatcagcccg atatttttca gcggggcaaa aatagcagat cgtgcgccta
    12001 ctactacttg cgcttcttcc cgctcaatct tgcgccattc atcatatttt tcaccttggg
    12061 aaagtccgct atgtaagacc gcaactgatt ctccgaagcg tcctttgaat cgctcgacca
    12121 tttgcggagt caatgcgatt tcaggcacta acataatagc cgttttattt tctgacaaaa
    12181 catcagcgat tgcctgaaga tatacttctg ttttcccgct gccagtgatt ccctccagca
    12241 aaaagacctg atctttttgc tgctgacctg ctgtgatgat ttgttcgaca gcattttttt
    12301 gttctgcatt cagttccaat gccgtcgttt ggtcaaatac tcggtcttta taagggtcgc
    12361 gataacgttc tgattctata aactccagcc agcccttttc ttttccttga ttcaagacag
    12421 cagtactgat tcctttattt ttgaaataag caattggttc ttcttggctc agttcagata
    12481 aacacaaaag aagttctttt tgtttcttgg cattttgccg taattttgcc cattcttctt
    12541 cgatttgctt ttcagttaac aaagaacgga ccaaacgaat cgtttttact ttatttcgtg
    12601 tatggacttc atatttgata tctactaatt gctcttggcg ccagcgaatc aattgagaaa
    12661 ggctgccttc ttcttctgct tccttccagg aaatcacttc tcgctgatca aacaatacct
    12721 gaacttctgg tgtgtctgcc aaaggataga tcaatttatc atattctgct ttcatcacac
    12781 tcggaagcat cgtttgtaga caagtgattt tgaatgcgta agtaattttt ttcatataat
    12841 cagctaatga aagtaattct tcattgacca ccggagcaag gtctagcaaa cgaataactg
    12901 ccttcaaaga tggattagct gattcttctg atttttggat agccataaca aatccttgga
    12961 catgacgatt gccattacca aaagggactt ctactcgcat cccgacttgc aaagctgttt
    13021 ccatttcaga gggaaccaaa taagtgaatg gctgatcggt ctgcatagta ggtacgtcca
    13081 caatgacttc agctgttaat gtcatggttc gtttcctcct ttttttgtct tttcttatca
    13141 agcgacaaaa agttccttgc ctattttact agagatcgat ggatttgtct tatcttctgc
    13201 caaatcctaa caaaaaatta acagcagata agaaaaaaaa gaactgtgtc aaaaagttca
    13261 gacacagcct tttctaccag acaataagtg gcgaatgttt gaaaaggaaa cagctgggtt
    13321 tgcttttctt tttagatgtt cgctctattt ttctttatta cattttttgc tcttcacgga
    13381 tacgtgattc caattcgtgt tgttcacgct ctttttgtgc ttgacgttcc tcttcttcca
    13441 tcttcaaacg ttcacgtttt aattcaggat gtggatcatt gactacattt cctgcatcga
    13501 tttcttctaa agcttgaccg acgcttttta ctgaatcaaa agaatctaga gtaggctgtg
    13561 cacttgcatc caattcatgc gcgcgtttac ttgctaaaat cactagagaa tattttgaat
    13621 ttacacgatc taatagtgag tcaattgaag gttttaacat cataagacta catctccttt
    13681 aacattttaa tatatttacc gattacgcga tctactcgaa aatgttcact ggcaataatg
    13741 tttttgattc gttcaactgc tagcggcact tcatcattta caacagcata gtcgtaaaga
    13801 gccatcattt cgatttcttc ttttgcaact ttcattcgtt tttcgattac ttcatctgca
    13861 tctgttcctc ggcctacgat ccgtgatttt aattccgcta aatctggcgg agtcaaaaag
    13921 atgaatacac catcgggcac tttttctttg acttgttttg ctccttggac ttcaatctcc
    13981 aagaaaacat cttttccttc atccaatgtc ttgttgacat atgacaaagg agttccataa
    14041 taattcccga catattctgc gtattccagc atctctccag cttcgatcat tgattcaaac
    14101 tcttctttgc ttcggaagaa ataatcaacg ccttcgacct ccccttcacg cattttacgg
    14161 gtagtcattg agatcgaata ctgaaaatca ttatcctcac tctcaaaaat tgcttttcgt
    14221 actgtgcctt ttcctacacc ggatggacca gacaacacga ttaataatcc acgctctgac
    14281 atgacgacgt cctttcaact agtctttttg tacttatcgt ttattttgca ctttttttct
    14341 gcaactttca aggttatctt taattatagc tagaaattgg taaaaaagct accctgtacc
    14401 aagaggtcgt aaaaaagctg tcctgcatca agtaataaga acagccaaac ataaacagct
    14461 tctcatgttt ttcgtttggt tggggttatt atcgcagatg caagcttttg aacgccgtgt
    14521 ataagaggtc gtgaagaagc cgattagttt cgagtatgaa gaaaaatttt gtgaaaatag
    14581 tttctcacat ttttgcataa atttggctta ataccgagaa aatggttttt gaacatcact
    14641 tattcaaaat aaaaaaagat tgtgacacga attttgtcac aacctttaaa tgtattccta
    14701 gagcaaagaa gctaagcgaa gaaatccaat tctgtttatt tattgtattt ctttgttcag
    14761 catccttgct aaaagcccat attggacaaa gtattcacta cacccatgat ctcatctttt
    14821 cgagaacgtg ctttttctgt cggatcttct acctcttctg tgtcaattcg aacaataaat
    14881 ttccgattca tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc gttctcatta
    14941 tagtctgcac aggcatcttt ccatacgtca aagccgtttt tttcagaagg gatatcccgt
    15001 ttaaccacag tttttccact taattcgtgg aacgttacgt cataatattt cattatctct
    15061 acctctttcg tcattcaata ttttgtactt gttctctgat tttttctaag atcgtcttag
    15121 actggacgac ttgattttta atttcgattg catttgattt ggaaccgatc gtgttgattt
    15181 cccgattcat ttcttggatc aggaaatcta attcccgacc tacaggacat tgtgcttcga
    15241 taagttcatc tagtttttgt acatggatcg ccagacgatc cagttcctcg tgaatatctc
    15301 cgcgttccaa caagatggct agctccgtca acaaccgttg ctcatcgact ttcgctccaa
    15361 gaaattcctc cagctttttt tgatacctta tttgaaattc cttttcatag atttctacaa
    15421 aactatcgag tttttttagt acttctttga attcttcagt attctttttc aacactaaag
    15481 ctaaagcttg accttctttt tctcgactcg cctcaataga atcaagtgcg cttttcaatg
    15541 tatctagagc taactgtgcc aattcttcca tgctctcttc cggtttttct tctaccgtta
    15601 cgaagttttc gtccagcacg aagcgttccg ccatccgact aattgacact tgattttgac
    15661 taccgtatcg ttgttgaatt ccgtctgtca attgagtgat aagtgaatca atcaattccc
    15721 agttgatgac gatctgtttt tcgtttttgc caatataagc tagattcaca aatacttcga
    15781 ttcgaccgcg gcttaactgc tgtttaataa gctgacgaaa atcgttttca acaaagttca
    15841 ggattttagg acttcgtatt tgaacatcta gaaaacgctg gttgacactt tttatttcaa
    15901 tatctaactg atacgctttt gtttcgcgga tggctttgcc aaaaccagtc atacttttca
    15961 ttcgacaggg tccttcctca ttacatattt tctattgctt tttgcagttg ttcaaactct
    16021 tcgttcgtca atgtcaggcc tttccccatt ttttcatggt ctggcgccca atctcgaaga
    16081 tcgaattttg gcgggcgacc attccagctg actaggttca gttctttacg ccaccccttg
    16141 ttgttttctg acaatatagc gatttcttct acgatttcat atgaaaattc ttgtgccatg
    16201 gttgttcctc cttgagataa tataaatata ggatcagtaa tgtgtcaact ggttgcttaa
    16261 gcaattcatg ctgcaaacga tccttgcgtt ttccttgtag cagataatca aaataaacag
    16321 ttttcgtgcg ctcattttgt attttttgct ggtattttcg ataccttcgc cattcttcag
    16381 tacttaattg accaatttgt gccaataaat catccggcat actttttata tcttgttggc
    16441 agatcatttg aatcatcatg tctacttgat agatttcatt gggcagtaaa aaatgttttt
    16501 ttgctaaaag ttctagcatc gccaatccgc atgtctctgg tacgaccctg ttcttatata
    16561 aattacgcag tttttgccaa aattttatgc ttttgaaaaa agataattct aactgaattt
    16621 cttttgttaa gtaagacaaa aatcctcgcc agtatgttct tgcaggttta acagaatgaa
    16681 atgtatacca gttcccatag taatagccta atcgtttttt catttccaga tagcctaatt
    16741 gtaaatttaa ttggttcctt tggctatcaa aaagcagaaa gctgcctagt gtccacaatg
    16801 tttgacattt ccaatggata aaaatgtctg gcatacgaat ttttttcgct gggcctggcc
    16861 cttgcacatc tacgacaaat gcctctgttg ctcctttgtt aatcgcaata tcaataggta
    16921 ttttgttgcg atacccaccg tctgcatatt tattttttcc gatctttcga taagccatag
    16981 cgggataaaa tgaagcagag gcgagtatcc aatctggtat ttcttctgtt tttgtctgtt
    17041 ggatatcagt cactacttcc gctagttctg gcagtttagt agacaccgta tataaacgta
    17101 ttttgggatt atgtttgagt aaggacaaat ccaaattgtc tttgaccatc ttttccaatg
    17161 gagcagaaga aaccccgcct tcaattagtg ctgttttcgt catttggcgt ctttctcgat
    17221 agaagcgttc acgaaggtct tcaactgcag ccatttctgg taacgcaagc acttgtcttg
    17281 tcgacagctt tttccataaa ttcacagctt ttttcatatc tccttgtaaa atcaatgcgc
    17341 cgttcaacgc acctaccgag gtacctgtga taatctcaaa gttgatatca tgttccttca
    17401 gcgcctgcca cacaccaatc tgataagcac cgtgcgctcc tcctccacct aaaacaagtg
    17461 ctgtatgata gaccagcttt ttatttaatt gtgtggttcc ttgatagccg tttttatgaa
    17521 aaatacgcaa ccattcttct gatagaggag attgaaataa aaaacaacag ttttctttaa
    17581 taaaaaaacg agcgcaagct tccatatacc gaaaaaattt tttccattca aaagtttcac
    17641 cttcaatcaa cagattatta aaaataacat ctcctctact tttagcaata gtaaaataga
    17701 cggtatattt tttcgatact gctttaaaag cacattgctt ttttgcagaa cgccaaaaag
    17761 cgtctctact ttcttgatga ctttgaaaaa atggaagggg ttgttttgct tgctggatag
    17821 gcgaatacag cgacattttc tgtgaaaagt aaactttttt tatctccatc gcgctctcct
    17881 ttttcttcaa gtataccagt acgtcataaa aaggtcatga gtctcgtatt cgactttgta
    17941 gattccataa gaaaacaatt cataaaacct cctttaaata aaaaaataca aaaatcacaa
    18001 aaattaattg aaaatgagag tgttttcaat gaaaacgtga ttttgtaatg gaattttaaa
    18061 ataaaagaaa caagcttttt taatgcaaat gttgtaaaat aaaagagatt aagaggaaat
    18121 aagaggagga acatgatgac tgagtcagtt tatgtacata ttgataccac aagtaatgct
    18181 gttttaacaa aagggctgac tgctactgac tttgcaaaca gtatcgtgca ttttcctcaa
    18241 aacttgctct tgcttgatcc atctgcttct gctggagaat atgaatccca tacagggctg
    18301 aaagtaatcc gtggtacaga aaatattcag cgtttctttt cctcttctcg taacaggcgt
    18361 ggattcgatg aattgaagtg gatcgacttt actgatttaa cgatgctgaa agaattgaca
    18421 cctttagaaa tttcagaatt attatatttt ggtcatatga aaacacatct tcattcgccg
    18481 tttttttata aactacagaa taattttgta tacttcgatc ttaatgatca gctgaatcgg
    18541 atttattatc gctatttaga tgaattttat aggatttttg ctagtaaatt aagttcgatc
    18601 ttgtcagata agatcaatgt aaaaaaatct tttttcaaaa aagaaacacc tgttgaaaaa
    18661 atcagtgtgg aattattgaa agaaatgcgt aatttgatgc aggaagggat cattttttct
    18721 tttgatcaag ttggtttgat cgatggtaaa tatcgtatcc caatctatgt gattgaagat
    18781 tatatttgga aattaaaagg cacccggtat cgagaagagg aaatcatcgc tactttaatc
    18841 tactctgtca ccgatcgaca ctggcaattg gtagaagatg ccgatctttc ttatctgcca
    18901 ttgaagtaga tctatttctt atcaaaaaat aaaaaggaaa caagaaacta ggacatgatt
    18961 ctgtctcaag tttcttgctt cttttttatt tacagctcct gtcgtttatc tggcacaaaa
    19021 atcgaaggag tcgtttgttg gatccctaat aaaacgatga tcgttacaat agctgtactc
    19081 aacccgctgg caccattcat tacgaaagaa aagagccaag gattcatccc ccatagtgca
    19141 tacgctcccc agaaaaccac tcctgcaata aaatgccaaa aatatctgga aagtgcgcca
    19201 atcaatgcac caaacacgat gctttttcta atttgtttcg tattatcgtc tacatagcct
    19261 ttttggacat ttgaagccat gagccctgct gcgcctgcaa aagtataggc aatcgggtac
    19321 tcaatcaaca cttgaacgac acttaaataa taaacttgtc ccaacggaaa atgtaggatg
    19381 ccccaaatga aacctgccag catccctgcc tttgtccctc ttcttaacgc aaaaagagtc
    19441 aatggaatct gccctaatga aatagaaaaa ctgctgccga tctggatcgg aatcaacgac
    19501 aacaccatcg ctaacgctgc aacaatcgtt ccttctaccc aaactctatt ttttcccata
    19561 aaaaaacttc ctcctttgaa taaaaaagtc acaaaggagg aagcagcttt cgaaaatagt
    19621 caaacagctg tttattcaca attcctacgc tcgcataacc gatacaggta cgaagggttt
    19681 agaaatagat tcaatctcag cccaattgga ctcccctttg tgagtttatt tattatttat
    19741 tttttgtttc aaacaatgga ctaactggtt tgttttcatg gatacgtttg attgcatctc
    19801 ccattagtcc gccaacactt acttcatcga ttttgtcgat tttacgatct tctggaagat
    19861 agatcgagtc tgtaacaact agacgctcga tagctgaatc ttctattctt tgtaaagcag
    19921 ggccggataa tactggatgt gtacatgaag catacacact agttgcacca gcttctttca
    19981 aagcattggc agccaatgtg atcgtacctg ctgtatcgat catatcatcg atcagtacac
    20041 aagttttgcc tttgacttgt ccaatgatat tcattacttc tgcaacattt gcttttggac
    20101 ggcgtttgtc tatgatcgca atcggagatt tcaaaaattc cgctaattta cgtgcccgtg
    20161 tgacaccacc atggtctggt gaaacaacga caacatcatc gcctttgatt cctttttcta
    20221 agaaataatt ggcaataagt ggcgcaccca tcaaatgatc gactggaata tcaaagaaac
    20281 cttggatttg gactgcatga aggtccaaag tcagcattct tgttgcacca gctttttgaa
    20341 tcatgtttgc tactaatttc gctgtgattg gttcccgtgc acgtgcttta cggtcttgac
    20401 gcgcatagcc ataataaggc atgactacgt tgattgtttt tgcacttgct cgtttcagtg
    20461 cgtcaatcat gatcagcaac tccatcaagt tatcattgac tgggctgctc gttgattgaa
    20521 taacatagac atgcgatcca cgaatacttt cttcaatgtt tacttgaatt tctccatcac
    20581 taaattgagt aacagatgat tttcctaact cgacgccgac tgctgctgca attttctctg
    20641 ctaatgggcg attggaattt aacgcaaaaa tttttaatct tgggtcaaag taatgttttg
    20701 acatggggac ctccactttc tttttcacaa ctctagaatt ctacctaata aatagtagtc
    20761 atatttttta aataaatcaa gtgattttgc aaaagtctta gtttagataa gggagttttt
    20821 ttgcatatcc ttctttattg acttgtcttg ctctcgcaat tgccagcgca tattctggaa
    20881 tattgtctgt aattgttgac ccggcagcaa tcgaactgtt tttagctacc tcgacaggtc
    20941 cgatgatatt cgtgctagaa ccaataaagc tgtggtcgcc gacagtcgta tgatgtttgt
    21001 tctttccatc atagttgacg aagacaacac cacatccgac attgatttct tctcccagcg
    21061 tagcatcacc aacataagtc aagtgaccga ctttagttcc tttgccgatt tgagcatttt
    21121 tcacttccac aaagtttccg atatggacgt tttcgccaat ttcagctttt ggtcgtaaat
    21181 gtgcataagg tcccacatct gcatgtttct tgacatggct gctttcaatg actgagtttt
    21241 caatgacgac gtgatcctca atcctactgt ctacgatttt tgaatgtgac ccgatgacac
    21301 aatcagaacc aatgactgta tttccttgga tttgtacacc tgcttcgatc aatgtgtctg
    21361 gaccgatttc tacgcctgca tcgatgtatg tcgttgctga atcgacaaag cttacgccat
    21421 tgaccatatg catctggtta attcgctttc tcatgatctc atttgcttta gctaacgcga
    21481 tccgatcatt gactcccatt gattcttcaa aatcatccgt ttgataagca gcaactgtat
    21541 ggccttcttc ttttaagatt tcgatgatat ctgttagata gtattcacct tgagcattgt
    21601 ttgttccaac tttgcttaac gcatcgaaca aagcttcgtt gtcaaaacag taagttcctg
    21661 tattgatttc ttgtacaaga gcttcttctg gtgttgcatc tttttgttcc acgatttttt
    21721 ctacgatccc aatatggtca cggatgattc gtccatatcc tgttgggttt tctgcttgtg
    21781 ccgtcaagat cgttgcactt gcattttttc cttggtgata ttcaaaaaga ttgttcagcg
    21841 tttcagtagt caataatggt gtatcgccac tgataactaa cgttgttcct ttttttcctt
    21901 ccaagaaaga agcagcttgc aaaacagcat gtcctgttcc caattgttct gcttgtaacg
    21961 catatttact gcgttctcct agttgtgctt taacttgttc tgctccgtgt ccaacgatcg
    22021 taatcacttc atctggtttc gtttcactga cccgattaat gatatgttct accataggtt
    22081 taccagaaac gggatgtaaa actttatata attttgactt catgcgggta cctttccctg
    22141 ccgccaaaat aattgcataa cgtgcgtcca actgcgtcac tcctaaatat agtttgctga
    22201 tttcagttta tcccagtata cccatttttt caacaaaaca agtgattttc ttttatccca
    22261 gcgttttctg gataaaaaaa tagatagacc ataacgggcc tatctatttg gtaaccggtg
    22321 ctctcctgcc aactcctcgg tcacaagtca cactactcat aaaacatgaa aagctgtttt
    22381 ttgaaatcgt ttcttgtgac ttagagttaa acggtaggct tacaacctct tcattaaacg
    22441 tttttcaatc agctgacctt cggtattagc tcaataaacg gcaaaatatg aggagcaatt
    22501 tttccgttta ttgagctaat actcaggaca ggacgctgat ttcacaacct ctagataaac
    22561 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgaaaaaatg tggaaagcca
    22621 ttttcacaaa atttttctta ctactcgaaa ctaagcggct ttttcacaac ctcttattct
    22681 gaaaaataat ttcctggcac aaccgtaata tttttcgttt gagtatctac atctttgaca
    22741 taaagcagtg acgtataatc atcgatggcg cgatgaccgt tgaattttga ttcggcaaat
    22801 acagtgatgc caacaagttc tgattcgaat tcttcaatca agctcttcat tccgttgact
    22861 gttccgccgc ctttcatgaa atcatccact acaaggacac gtgaaccacg tttcaaactt
    22921 ctcttagaca gttccatctt ttcaattctt tcagaagatc cagacacata gttgacactt
    22981 actgttgatc cttctgttat ttttgagtcc cggcgtacaa taacgaatgg cgcattcaga
    23041 taataggaca cagcttgggc aataggtacg ccttttgtgg ctacagtcat tacagcatcg
    23101 attttttcac caaggtattt agaagcaatg attcttccaa cttgacgcag cagttcaggt
    23161 tctcctaaca aatcagagag ataaacatat cctcctggaa gcagacggtc ttgttctgat
    23221 agacgttctg ctagagattc aatatatttt ttcgcctctt catatgaaat ttcagggatg
    23281 aacaatacac cacctgcagc acccgggatt gtttccaagg ttccataccc tcgttcttta
    23341 aatgttttct tgatgattcc caaatcttca ctgatagaag atttagcaga ttcataacgt
    23401 tcggcaaagc ttgttaagga aatcaattca tgcgggtgat tcaataaata ctgtgtcatg
    23461 tcaactagac gttcactgcg acgtactttc acctattcca actcctttta agaaatttta
    23521 tcgttttaca ttatataagt ttttcttcaa aaattcgagc attttatgaa aaaataagaa
    23581 aatagttcgg tatttattaa aataaaaaga agcctataaa aaatttttcg tttttataag
    23641 gcttcttttc tttataattt agttattttt tcttctgaag gcaaccaata ttcgtttgat
    23701 gatatttaca atcaggaaca aagcaataaa gactaatgtg attgttgctc ctggaggtgt
    23761 atctagataa aacgagctag ttagtcctga cagcatccct agaaatccta cacaaacact
    23821 gatagcaatc acgacattga agcttttgcc taatcgcata ccgattgctg cgggaagaac
    23881 catgattgcg gatatcaaaa gtgcaccggc aatcgggatc atcacagcaa tagccacacc
    23941 agtaatcaca ttgaacatca tcgacatcaa atgaacgggt agcccatcca cgtgggcggt
    24001 atcttcatca aaagtcagaa catacatcgg ccgtttgaat aaaaagaaca atataccgat
    24061 ggcaacgaat aagattccta aaaagatgac ttgctcccat gtaatggtca cgatcgaacc
    24121 aaacagatag gattggatac ttgttgctga attccctccg ctcaaattca tcaatactaa
    24181 cgctagcgcc aaaccaccag ccattaaaat ggcaatggag atttctgaat aggttcggta
    24241 gatcgttcgc agatactcca atatcacagc tgctaataca acgactagca tggtcgtaag
    24301 tgtaggatta aggttcaaaa agaatcctaa cgctacccct gctaaagaga catgagaaag
    24361 cgtatcagcc atcagcgatt gtcttctgat cactaaaaac acacctagca tcggggcgat
    24421 tccggcaatg aagatagcgg caagaaacgc tcttctcatg aattcatatg aaagcaacgc
    24481 cattgagaat cctcctttct caccagtcgg atctgtctgt ccgcatactg tttgatatct
    24541 tcatgatcat gggtgatcat tagaactgat ttcccatgga catgcgcact atgtcggaga
    24601 agacgataaa attcatttct ggattcttca tccatacctg ttgttggttc atcaagaaca
    24661 aataagtcag ggtcagtagc aaaaatacgc gctaaactga ttcgctgctt ttgtccgcca
    24721 gacaattcac ctattcgccg atttcgcata tcccacatcc ctacagcttc taaagcacgc
    24781 tgtacatgtt gatgatcttt ttcagagaga cgcttgaacc agcggcctct tggatatctt
    24841 cctgaacgga ctaattcgat caccgtacta ggaaaacctg cattgaaaga tgcaacttgt
    24901 tgaggaatat aaccgatact taattttttt ccttcttgat ttttctcagc aatctttatc
    24961 attccttttg aaggtttcag taatcctaac gatgctttca ccaaggttga cttggctgct
    25021 ccattttctc cagttaaaat gacaaattca cccgcatcta catgataaga gacatcttct
    25081 agtacaggtt catcatcata gtaaaaggtc aaatcagata tttcgatata acgcacctta
    25141 caacctccat tctaaaactt aattgatact ttcttttaaa gctttcagat ttttttccat
    25201 gacagaaata taattttcgc ctgctttgat ttgttcatta gtcaagcttt ctaaaggatt
    25261 caacacttct agcttcactc ctgtttcatt cgacaatgtc tcagctacct tcgaagaggc
    25321 attttcttca aaataaatca ctttgatgtt gtttttcttc acaaattctt ttagctcagc
    25381 taagcgactt ggtgtgggtt cctgatctgg tgaaacgccc gaaatcgcta cttgttttaa
    25441 gtcgtattga ttcgctaaat aagcaaaagc agcatgctga gtgacaaatg tgcggtttgt
    25501 tgcatctttt agctcatcgg agtatttttg atccagtgct tcaagctttt tgatataagc
    25561 atcggcattc ttcgtaaaag tttccttttt atctggatat ttttcgccta attcttcagc
    25621 aatcgttctg acttctttta tagctaatac cggatcgagc caaacatgtg gatcgtattc
    25681 atgtgaatga ccatgccctt cttctgattc ttcatgttcg tctgcttctt gcgattctaa
    25741 acggtcgatt cctttagctg cttcgatcat caaggtctgt ttgctatcca ctgaatcctt
    25801 taacgaatcc acaaaaaatt ccatgtctga actattatac acgaaaacat ctgcatcgga
    25861 aatttctgct ctttcttttg cacttggttc gaaatcatgc ggctccgttc cagcagggat
    25921 caaaagtttc acgtttcctt catcccctac gatctctttt gtaaaatcat acatcggata
    25981 aaaagtcgta acgatcgtca aatcctcttt tttgtctgct tccttgttcg tatttccaca
    26041 tgctgcaaac agtaaagcac ctatcattac cgtagctgtc aaaagatatt ttttcattta
    26101 tatgctcctt tcaatgaaca aatcggaatt ttacgattta ttaacgtgaa ctaatgtatc
    26161 aaaataaaaa aacacagtca atagaaatcc atcaatttct gatttaagca tgtatattct
    26221 tgattaaatc gtaaaatgga tgagttgctg ctcatatcta gccatttcac tacaataaaa
    26281 aactgaagaa taccatttta ggattcctca gtttttcatc acctatttta cttcttatat
    26341 tttattgcag ggttcgtact aaataaactt cctcacaaaa accttttaac ccattatata
    26401 ctctctgcgc tcttgaataa tgttgacaca aggcaaaaac ggttggtccg cttccactca
    26461 tcaatgcagc atctgctccg tacttgatca tacgctcttt gatttgatgg atgactggat
    26521 gccgctttcc cgttatatct tctaaactgt ttcccatttt tttgatcatc tgctgataat
    26581 cttgctctcg gatggcttca gcaagtccgt ggatgtcttg atgttggatg gcttgcgtat
    26641 ctacttctct aaacactgta cgtgtagaga cactcattct tggtttgacc aacacaaccc
    26701 agcactgtgg catagaaggt aatacttgga tttcttcccc tttgccaccg acaaatgccg
    26761 tacttccgta gacacagtaa ggaacatctg taccgacttc caaaccgatt tctgccagtt
    26821 cttctaacga acagcccaga ttccataatt ggttgatccc tcttagagca gctgcacagt
    26881 ctgtacttcc tccgcctaat ccagctgcta ctggaatatt cttcttaaca aagatcttga
    26941 caccggtttt gatattgtat ttatttttta gcaataaagc agcttgataa acattgttcc
    27001 gttcatctac cggtaaaaat gctttatttg tttctacaat gattttgtct tctgttattt
    27061 cttcaaaaaa tagatgatct gccaagtcta cgctcgacat caccatttcc aactcatgat
    27121 acccatcttg ccgtctatat aatacatcca gtcctaaatt tatcttggct ggcgcttttt
    27181 caatcaattc catcctctca cccattcgaa aagtctaagt cgcaagtatt ctatcatagg
    27241 gaaaaataaa agtatagctt atgtctgcct agtggttgat tttgtcatcg tttcaacagc
    27301 tttcacagaa ttttctcttc ataatccaaa caagttctag tgattcttat aattctgtga
    27361 gcacttcttc tgccaaactc ggaacatttc ctttttcttt tcctatcaga ctgagtagct
    27421 gcatggtagc aaatccgaaa ttacttcctt tcctataagc aaaataacgt ccttcccaat
    27481 gatcgacata ttttaatttc ttttcataag cagactggaa ggaatagaaa aaattaccat
    27541 aattatatac gatatgcatg actttttctt gaaagaaaga aaaatctgtt tctccaacat
    27601 tagctaatgg agctaagcct agattggctt ccgagtagcc cattttctcg tatacaataa
    27661 acagattggc taacagatag tcagctaatt cctcagattc tttaggtaag acccgcaaca
    27721 aatcatatcc cgcctgcttc ttcgtcatcg gctgagcggt gatgaagcca acgacttttt
    27781 gatctttacg gacaataccg acaccgctag ataatacata tgatttatca aatcgtccca
    27841 cagagaagta tttttctcgt gcaccttgaa gccatttttg tgaaacttca tttacagctt
    27901 gatgtaattc ttccggcagt tgatcgtacc aagtaaaatg gtatccttct ttcatcaaac
    27961 gttgatattc aagttgctga ctcagccgag acaagtcttc tgttgctaga tcaacaatcc
    28021 ctgcttcacc tactttcgtg aagtgaaaac ccaattcatg aagcatcact acgtagcttt
    28081 ctgatatctt ataaaacgca agctgatagc ctaaacaatc tgcttgtttc ataaactgat
    28141 tcgttgctgc ccgcatcatt ttaggattgc caactggatc tcctaaaaca aagcatttgt
    28201 ttcctttgat ctgataacca aaaacgactt gatcttggtc gtcttcttgg taataaaaat
    28261 aactgtaacc tgacaagcga agctgatggc ttgtagctgt gcctccgtat gtttccaata
    28321 aacggtcaaa acggtctttt tcgtactcac tgccaagctt cgtagtatct tcagctaaat
    28381 attgataaag cgccactaaa gctagcaatg agatgcccaa accaataagc cctgagaacc
    28441 acacatcatc tgaaggaaac aaaataaaat gatttcctgt tattcgaccg actaaaaagc
    28501 gtccgctgtg atagcctgca attgcgtaaa cgataaacaa aaagccgaac aagctagcat
    28561 caaagattat agctccccaa gaataaacga attttgttcg ataaaattct tttctggata
    28621 accacacagc taacagaatc aagacatata ccaatatcaa ctgccaagat aacgtacgtg
    28681 tgattgtatt taaaatacca aaagatagta aagcaatcgt aggcagaaat gcctttttga
    28741 cttttactga aagtgctcta gcaaggccga gtaataaaaa gccaaccaat agattcactg
    28801 tctgatctaa aaaattgaat gagaacggca ataatacttg aaatagccga ctgattgcag
    28861 acagattagt gattgttgat agaagtacca tcatgatccc cgcaaaatag acagcactta
    28921 cgagcacgat atgcgcaacc ttttggaaaa acagtcgcgg aatgttatct aaaaatcgat
    28981 tgattttgac tcctgtatgt gtgatgaaca atagaagtcc cgtcataaaa ggtagaacat
    29041 aataaaacaa ccgataatat aatagccaaa ctactgctac atcttgccga acagacaatt
    29101 gactcattcc taagatcatc aaaacgtcaa aagtccccat tccacctggg accatcgtca
    29161 gcattcctat caatgtagca atcatgaaca taggatagat cgaaagcatc gaaatatcta
    29221 ttctcatcaa cttgccgatg atcaaaaata ccgcaagggc tcccgtccat tgtccaaagg
    29281 atgccagtag cagaccaagc aagccttttg gataaaactc gaagaacaat gtctttctct
    29341 ttagataagc aaaaaagaaa atgactggtg ctaaaaaact accgccgaaa agccagatcc
    29401 aatagcttcg aaaaatactt tctgactgaa taaaaaagac atcaataaat gtaagaaacg
    29461 accatacaga caaaccagtc agcataaata gtgcgacttt tgatacagtg gctaaaacca
    29521 tttttctatt tgtctgcttt ccataaaaac tagcccgcag acttgctcct accacaccac
    29581 caaaaccggc gagattgttg atcgtgtttg ttacccacgc agcaagaaga aactcttttc
    29641 tttccatttt tggttttcct tgtttttcta agactctgat cgtcacccaa tcatacaaca
    29701 acatcggagt gacacccagc agtccagcca gaatcatgag caggatcgtc atgcggcttt
    29761 gttgtcccat cctttgccaa atatcttgcc aactcatgcc attagcaata tttgcgactt
    29821 gatttgccac aaagactaac acggagccga aaaaaatcag ttttaaaatc agactatgtt
    29881 cctttatcca ttgcagcatc aagtatcgtt tatttttcac attttcccat tcctaacttg
    29941 tatacttttg tgtcataccc caatgttatc ttatttattt taatttttcc actttagacc
    30001 tgttctttgc ttgcgatgat agaaaaagaa agatatccta atgctaaagg agaggataac
    30061 atgaaagtag gatttatcgg cacagggaat atggcacaag ctattattga tggtttatta
    30121 aaaaaaggaa cagtctcgaa agaagaggtg ttcgtttcca gtggacattt tgaaaatgca
    30181 caaaaatttg ctgctgaaac tggcgtccat gcatgcaaaa gtaatcagga agttgcatca
    30241 caatcagata tcgttctttt agccgtgaaa ccaaaaatca ttgaaaaagt attggaagaa
    30301 ctaagagaat atagccagaa agtcttattc atttctattg cttcaggaaa atcattaata
    30361 gatttagaaa cagcgcttga agatcctcag gcctccatcg tacgtgtgat gccgaatgtc
    30421 aatgtcagcg taggagcagg cgtttctgcg atttgcaaaa atgaagctgc cacggaggaa
    30481 cagcttcagc aagctacgat gatttttgaa gcaatcggca gcgtctatcc tttagcagaa
    30541 tcagatttca gtacattcat tggccttgct ggcagctcac ccgcattcgt ttacatgctg
    30601 atcgatgcga tgggacggac tggtgtactg aatggtttgt ccaaacagac agccactgag
    30661 attgctgcaa aagcaattat gggttcttgt caaaaattcc tttcaagtga taaaaatcca
    30721 tgggaacttg tagaccaagt ttcttctcct ggcggtacaa ctgttgcagg tgtagttgcc
    30781 ttagaagaag caggattcat cccagcaatc atcaaaggaa tcaatacgac gatcgagaaa
    30841 gatcttgaga tgttgaataa aaaataatga tagcaatgtt ttttcgtgcc ttacaaaaaa
    30901 agaagagctg tggcttttgc cgcggctctt cttttgtatt ttttattatt tagcataatc
    30961 agttgcacga gtttcccgaa taacgattac tttgatgtgt ccaggataat ccagttcttc
    31021 ttcgattcgt ttacgaatat cacgtactaa acgaacagcg tctaaatccg agatttcttc
    31081 tggcttgacc attacccgaa cttcgcgtcc agcttgaact gcgtaacttg attctacacc
    31141 ggcgaagctg ttcgaaatat tttccagact tcttaaacga cggatgtagt tttctaatga
    31201 ttcacttcgc gcaccaggtc ttgctgctga gagcgcatct gctgcagcta caaggacaga
    31261 gataacagaa gtagcttcca catctccatg atgagaagcg atcgcgttga ttactactgg
    31321 gttttctttg tacttggcag ctaattccgc accgatctca acgtgagatc cttcaatctc
    31381 atggtcaagt gctttcccaa tatcatggag caacccggca cgttttgcta attggatatc
    31441 ttcgccaagc tctgctgcta agataccagc taattttgca acctcgaccg aatgcttcaa
    31501 gacgttttgt ccataactag ttctgaatcg cagacgtccg atgatcttga ttagatctgg
    31561 atgaagtgta tgagcaccga cttcaaacgc tgcctcttcc ccgtattcgc gaatacgttc
    31621 atccatttct ttacgagatt tctcgaccat ttcttcgata cgcgctggat ggattcgtcc
    31681 atcttgtatc agtttttcca aggtcatccg tgcgatttct cttcggattg gatcgaatcc
    31741 ggaaagaaca actgcttccg gtgtatcatc gatgattaaa tcgataccag tcagcgtctc
    31801 aagcgttcga atattacgac cttctcgtcc aatgatacga cctttcattt catcattcgg
    31861 aagggtaacg actgaaacag tcgtttctga aacttgatct gctgcgcagc gttgaatcgc
    31921 taatgataat aagtttttcg ctttgcgatc tgcttcttct ttggcacgct gttcagattc
    31981 ttttaccatt agagttaact catgattcag ctcttcttca gtcgatttca tgatgacatc
    32041 tcgtgcgtcc tcttttgata aaccagcaat tttttctagt tctttttgtt gatcttcaat
    32101 cattttttca acgtcttttt ctcgctcttc aattaattgc tgttttgaac taagtgcttc
    32161 ttctttactt tcaagtgagt tctcgcgttt ttctaacgag tcatctttac gattcagcgt
    32221 ttgttcgcgt tgaatcaaac gattctcttg agattttaat tctaacttac tttctctcag
    32281 ctcactttca acttcagaac ggtatttctg attttcttcc ttagcttcta acaatgcttc
    32341 tttttttaga gtttctgctt ctttttttgc actatcgatg attccagcag ctgatgattg
    32401 tgcaccagct atttctttct cgtgacgtga ctttgcgacc ataaacccta aaccaagacc
    32461 gacaattaaa ccgatgatag cgaggagaat gttgaatacc ataaaatcca cctccatact
    32521 atctttttca tttttatcat ctatacagct tctatgataa attattctaa actcgtcaat
    32581 atgcatcttc gcatatatgt gtacaaaaaa catgcacaac tttattctaa tgtttgtacc
    32641 ccgtagtgtc aactttaaat aaacaaagaa acggctttct acttattcat taaacgatgg
    32701 tttttaaaat aaatgacact agtttcatat aggtaaacct ttaaattgag aaacttttct
    32761 attaaattat tctttgtaga tgaacagata tttttatttt atgtacccac tcgtataatt
    32821 aaataactca cgatttcttt attcaataaa aaaacggcag gtcaaggaga gtacctactc
    32881 ttcagaccag ccgtttttgt ttatcataat ttttattctt ctactaaagg caattcttct
    32941 tgtccttttt catcttctgg gacagcgact tcttcaccaa taccataagc tgctctaact
    33001 agagcagaaa cttctgccat catttcagga tgattagcca tatagatttt agcgttttcc
    33061 cgtccttgac caatccgatc ttctttataa gaataccaag caccgctttt atcaacaatg
    33121 tctttttcta ctgccatgtc tagaagttct ccttcttgtg agatccctaa accatacatg
    33181 acatctactt cagcgatttt gaaaggaggt gctactttgt tttttacgac tttgatcttt
    33241 gtacggttac caacaatatc tgtaccttgt ttcaattgtt ctgcacgacg tacttccaaa
    33301 cggatcgttg cgtagaattt caatgcgcgt cctccaggag tgatttctgg attcccaaac
    33361 atcacgccta ctttttcacg gatttggttg atgaaaatag cgattgtttt tgttttgttg
    33421 atcgaaccag agagtttacg caatgcttga gacatcaaac gtgcttgtaa cccgacatgt
    33481 gagtcaccca tttcgccgtc gatttctgct cgtggaacta aagcagcaac tgaatcgact
    33541 actacgatat ctacagcccc acttgatact aaagcatcag cgatctctag accttgttct
    33601 cctgtgtcag gctgtgaaag aagtagttca tcgatattca cacctaattt ttgtgcatat
    33661 tgcggatcta acgcatgctc agcatcaatg aaagcggccg ttccgccatt tttttgtact
    33721 tctgcaatag cgtgtagtgc aactgttgtt ttacctgaac tttcaggtcc atatacttcg
    33781 atgatacgtc cacgggggta accgccgaca cctaaagcga catctaacgc aagagagcca
    33841 cttggaattg tagagatttg ttggtcgatt ttttcgccca atttcataat agagccttta
    33901 ccatagctct tttcaatttt tttcagtgca gcatctaagg ctgctttacg atcatctgcc
    33961 aatacgataa tcctccttat atttacaaat cagagcaaca tttcattatc cttatcatat
    34021 cttttttagt gaaaaaaagc aagaaaaaac cgaacaaata ttcgcattta tcattttctt
    34081 aaaagctgtt ttctcaaaag atcaaatcct tgcataaccg cattatatct gatagcagca
    34141 cgatctctgg caaagtgata tttttccgaa accacgccgt ctttagatga aatagcgatc
    34201 catactgttc ctgcagattg accttctaac gtgtccggtc cagccactcc ggtgaaagct
    34261 acactgtaat ctgttcttgc tatctcacgt gcttgaatgg ccatctgctc cacacattct
    34321 ttgctgactg ttccatattc tgctaatagc tccggatcaa tctccagaaa accagctttt
    34381 gtttggagcg agtaggtaac gaatccgcct ggaaatactt cagatacacc agcaatgtcg
    34441 cctaatgcgg cttggaaggc gccggctgtc aaactttccg ctgctgttac agtctgcttg
    34501 ttttctttca gtagttccac tacgactttt gctagcgaat tatcatcgcc gtatccataa
    34561 aaatattccc ctactcgttc ttggattttt tcttctaatg ctaaaagagc ttgattccct
    34621 gcctgaacat catttgtttt cactgtcagt cgtaatgtca cttcatttgg tttggcatat
    34681 ggtgcgatcg ttggattgat ctgtgtctca atcagatctt ttagctcagt gaccagtcgc
    34741 gattcaccaa tcccataaaa gcgcaaaact ttagaaatta gtttttcttc agaaggaaaa
    34801 cgttcctcta gtaaaggacg tacttgttcc acaaacattg gtttcaactc attaggcgga
    34861 ccgggaagaa ggatatacgc atgtgtatct gtttgataaa aagtccctaa agcaagccct
    34921 gtacgatttg gtagaggaac cccgccttcg ataatctggg actgttgcag attgtttttg
    34981 gtcatttttc gatgggttgt ttcaaaatat gccaataatt ttttgtatcc ttccgtattt
    35041 tgtatgagag acttaccgag gtgagccgca gttgcttctt ttgtcaggtc atcttctgtc
    35101 ggtcctaatc cgccacataa gagtatgagt tcacttcttg tttcagctag agcaatcaac
    35161 tcttctaaac gctgctgatt atctcctaca actgtttgat aataaacttc gatacctaga
    35221 tcagctagct gttctgacaa gaaagtggca ttcgtattca cgacttgtcc tagtaaaagt
    35281 tctgttccga cagctattat ttctgctttc attacttccc gctcccttat atagtatacg
    35341 ctgtattttt atattttttt atttaaaaag agggcaagga gctgtgacaa aaatcttgtc
    35401 acagctcctt gttccaaata aacagtgttc tcctgccaaa acctcggtca taagtcacaa
    35461 tatacataaa acatgaaaag ctgttttttg aaattatccc ttgtgactca gagttagacg
    35521 gcaggctcac aacctctcca ctaaacgata cttacattga tcctttgaac acaccgctgt
    35581 ttttgatgaa gtaatccaca cctgaataga tagtaaagat cagacaagca taaagcatga
    35641 tttgatctac tggaataccg atcaggctga atgggatatt attgataaac agcaagataa
    35701 tagcaaccat ttgtgtagcc gtcttgactt ttcccggcca agctgcagcc atgacttctc
    35761 catgttcgac aagtagtaaa cgcaagcctg tgacggctaa ttcacgacag acaatgatcg
    35821 ctactaccca accaggggcc tttccttggt caaccaacat gataaaagca gtcataacta
    35881 gcattttatc tgccaacggg tccgcaaact tgccaaaatt agtgacaagg cctcgcgcac
    35941 gggcaatttt tccatctaac cagtctgtga tactagctaa agcaaaaata actgcaccta
    36001 ctagctgtgt gacagccagt gtggtgtctc ctacgttaaa ttgtccccaa tctaaaggta
    36061 aacctacaac caaaatgaaa atcgggatca tacaaattct aagtacagtt aatttatttg
    36121 gtaaattcac actatcgact cctttcttcc ctattctacg tgactatcag cgaagtttcc
    36181 accgatatta tttacttttc caaatgaatt tattcaaagg agtattttta ttgaaattga
    36241 tctttttgaa ttaacgaaga aggaccgcga cacagatttg tcacgatcct tcttttttat
    36301 acctacaagt aaacagatgc gtaaactaat ttatacgatc attctgcgta ttgaagagta
    36361 aggttcacat tttttggact ctgattgttt tcccctggat taactggtac ggattctcca
    36421 ttcgctttga tcgttgcatt gcttgctgca ccaatcgtga tggtcgcatt tgttgctgtt
    36481 tccggtaaca cggtactctg tgtttcattt gctgccagtg tcccttgata aacataggca
    36541 ttgtctacta gcacacctaa ccatacacga ttgacagcag taaattcaag ggttacaggc
    36601 ttggtagcgt ctgtgatatt tactgcgatg gcactgccag aatcttctcc tgtagctatt
    36661 gccattttct tttcttcact tgattttgtt gttgattcta ctgtagaaga ctctttggtc
    36721 tgcgtgcttt gctctgagga agaggactcc tcgactgatc cttctacttt aagcgaagaa
    36781 gctgtgtctc cgattattgg atcagcttga cgatcttgcc aagtcatata gcccacgaca
    36841 acgacaatcg ccaaagcaac caatcctaat aaaatgacag gaagacttgt ccaaaagcga
    36901 cttggatgcg attcttcctg atgcattgct tttctagaag tacttactgt ttcaggctgc
    36961 ggacgttcag ggaccgatcc tgtcaatttg cttttcccat caaaaacatc gactaaatgg
    37021 tctccatctt ctcctacagc ttcagcgtat tgccgaataa atgcccgcac gtaaaattta
    37081 ccaggcaatt ggtcaaaatc atcattgtcg attgcttcca aataacgttt ttgaattttt
    37141 gtaatctgct gtaattcatc taaagatatt tttttattca ggcgtgcttg ccgtaatttt
    37201 tctccgatgg tttcaaaagc cactcgttca tcttctccag tctgtttgat tctccgaagc
    37261 atctgctgtt gcaacgaaag acgttatgtt tgggtgctgt gacaaaagtc atgtcacagc
    37321 tccttttttc cgaataaaca ttgttctcct gccaactcct cggtcacaag tcacaatatc
    37381 caataaatat aagaagctgt tttttgaact tgttccttgt gactcggagt taaacggcag
    37441 gctcacgacc tctttcttaa acggtgttca atcagctgtt cctcggtatt agctcaaaaa
    37501 ccggcaaaat ataaggagat atttttccgt atgtttctct aatactcagg tcaaaacgct
    37561 gatttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagtcca
    37621 accaagttaa aaacattaag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    37681 gcagcttttt cacaacctct tggtacacgg tgttccagaa gtagcttctt cggcaataag
    37741 ccgaaatatc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    37801 tgaccacttc tgtcacaacc tctttgatta cttcgtttga ttttatttta atataagttg
    37861 attaaaagag agcatgattt gttttatttt ctcactaatt tttccaacac aactaagaat
    37921 accataaaag ctgattttgg aggaggttcc tactacaagt ctaaaacgaa atttaagact
    37981 tttcctcata aaatactggt ttattttggt ctcatgtaaa actcgctgaa ggcctcttca
    38041 cttataaatg cttccccagc tgccagtacg tctttcattt ggatcgaatc gatgatttca
    38101 ggaagatcaa ataaagttcg atcgccaaat aaactttgcg taaattgatt ggcaatatat
    38161 tcgatcgaat ttaatgattg gaaatattgt ccaagcattt tcttcttcag caaatcgaga
    38221 tttgtttctg acacttccgg atcatcagcg aatccaagaa tgatttcttt gactttttct
    38281 gctgctttct ctggttcgtc cgtatctccg ctgaagtccg caaagtgaaa ttcacgatcc
    38341 aagctgaatt caaaaccaaa gctgtcatcg ataattcctt gattgtacat cgctagatag
    38401 tttcgagaag tattgcctaa aatcatttgg aacaacagat tgattgctgt tttatagcgt
    38461 aacagttctg ttccttcttg cggcaatgtg tctaatcctt taataccaag tacaaattta
    38521 tctcgtgtaa tggctgcttc caatgcactt tgtttgatga tttcttttgt gttttcaggg
    38581 aaataacgta caatttcttg ctttggaggg aaattttttg cttcttggtt ttcccgaata
    38641 agtttcatca atttctcagg ctccatctta ccaactacaa ataaaaccat attgcttggc
    38701 tgataaaacg tccgatagca cgtatacaag tcttgagctg tgattttgtc gatactttca
    38761 accgtgcccg caatatcgat atgcaatggg tgtgtaggat acaagttatt tagtatgcca
    38821 aaaaacatcc gccagttagg gtcgtcttca tacatttgga tttcctgacc gatgattcct
    38881 ttttccttgt ttacagtttc ttctgtgaag taaggtgctt gtacaaaatc gatcagagtc
    38941 gtcagattct tttctacctg atccgtcgta gaaaacagat agctggtttt cgtaaaactt
    39001 gtgaaagcat tggcggaagc tccttgtttc ccaaataatt gaaagacatc accatcttct
    39061 ttttcaaaca acttatgttc taaaaaatgg gcaattccat ctggaacttt caccttttct
    39121 ttttcaccat agggaataaa ttcgttatcg atcgaaccat aattcgtact gaacaaacca
    39181 tatgtcttgt gatagtcatt tttaggcaac aaatacacgg tcaaaccgtt tggcagtact
    39241 tcatgataaa gtgtttcatt gatttgctca tattctgttt tattcatttg catttcctcc
    39301 tgctaaaaag aagattgcct gcaattcgat ttgctcagct acttgttgta tctctttgat
    39361 cgttacgctc tccatacgtt tcaaccattc ttcatcagat aattttgttt ccggcagcca
    39421 gctgtccaaa aaagaagctt cgattgccgc ttgcggagag tctagagaaa gcagatattg
    39481 gtttctcagc attgcttttg tctgagacag ttccaactct gttatttcac catttcgcaa
    39541 actttctaat tgctcgtgaa tcagatgaag tacttgattt cggttctttt catcgatacc
    39601 ggtttggaca ctcatgaatc ctcggaacgt atccacgcta ctagaggcat aataagctag
    39661 actttctttc tctctgacat tcatgaaaag tttagagtga ggaaaaccgc caaataaacc
    39721 gttaaagacc attagcgcaa aacgttctgg ttcatcgtaa tacacatttg tctgataagc
    39781 taggttcagc tttgcttgaa cgattggctc acgtacttgt cgttccttca cgatattcac
    39841 ttgaggttgg gtataaaaca gatctggata aactgtttcc cgcgactcaa atggtaattg
    39901 cccaactgct tcagctgctt tgtccggatc aacatcgccg acaacaaaaa tgtcgatttg
    39961 atcatttgcc atcatttctt gatagtagga cgctagcgaa ctagcagtga tcttgtccaa
    40021 atcttcgact acgcccaacc ctgggatctt ttgttccggg ctgttttcaa aataaagttc
    40081 ttgcaaggca taggaagcaa aagtttgttt atcttccttc aaactttcca aatatagacg
    40141 cagattgttc ttttccaagt caaacgtcaa ctgatcaaac tgatgatttt taatatttgg
    40201 ataaaataaa acctctttta aaaaatctac cgcttgagaa aaaaggtttg gatcattgac
    40261 atattcacca ttcacaaaac tgatccctgc gtttagccaa tgtaaatttc ctttttttct
    40321 aactgataat ccaaagcttg cgccataaag ctctgcaagt tgcgcactta actttgtctg
    40381 atccgaataa ttcaaactat ttgtttccaa catgctgctg agaagtgaac gtttcgtgat
    40441 gacttcttgt tgcagacgcg cagtaaaacg gataaaaatc cggatcgttt tgtattgttt
    40501 tgttggtaag acatgaagat tcacgccttc tgccaattga tacaccatat ttatatgctc
    40561 cttttcacag ttgattgccc gcttactctt ttaatgggtc aacctgcctt ttttacttat
    40621 tcctctgctt gaaatttata agttcaggca attcgccgtt ttttctaaat gatgttacac
    40681 tttatagtat agcatagatg ttttttatca aaagaaacag acggctaaaa gcgtatattg
    40741 tttttttatt ttttattttt cgatatacta aagacaaata aaagaaggga gggtcattca
    40801 tgatcaaaga attcaaagag ttcatcatgc gtggagatgt cttggatctt gctgtcggcg
    40861 ttgttattgg tagtgcgttt acaagcatcg taaaccaagt ggtgcaaggc ttgatcacac
    40921 cactaatagg ttggtttgtc gcaatgatca caggtacttc tgatttagaa ggagcacttt
    40981 caattcttga ctggtcgccg acaaaaggcg tcacatttgc gtttggtaac gttattagtg
    41041 ctatcattac atttctgatc actggttttg tcttattctt agttgtcaaa gcggcaaacc
    41101 gtgcgaagaa tttacgctcc aaagaagagc aagtggaagt ggaagaaact cctactgcag
    41161 aagattatct tagtgacata cgcgatcttc ttgccaaaca agtggcagaa gacaatacac
    41221 atccagtaga gcaaacaaat ctttccgata cttctaaata ataaagaatc taaaaaaaaa
    41281 gaaagccgat tttcagtgtc ggctttcttt ttttagattc ttaacttgct acttgttcct
    41341 ttttcatgtc gatatgtgga atatcatctt ccaaatacac atctgagatt gccttgaatc
    41401 caaacgactc ataaaaagct tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg
    41461 gaaaattttt ctggatcatg ttaatcgttt cttgaacaat ctttcttcct aaaccatctt
    41521 tacggttgtc ctgatgaaca aggacacgtc caaaatggat actctccgct tcttcatata
    41581 ctcgcgtgta ggcaaggatc ttcccatcag tatcttgaaa aaacgtatgc caagcttctc
    41641 catcaatgtc atcaacttct tgataaggac actgttgttc cacaacaaaa actgcgattc
    41701 gttctttgac gatcgcgtaa aactctgctg ttgacaattc ttcaaatttc tttacctgat
    41761 attccactaa gcaaacgccc cctgcttttt tattcttagc ataccgattc gcacacaaaa
    41821 aagcaaacga accttacgaa tctcaggctc gtttgctttt taaactgctt cagttaaaaa
    41881 ttcgatctca actgtccgag taagtacatc tgaatagctg taagatactc gttcaaaaga
    41941 attttcttca ggatccaaat caacaacgaa tactgaagga taggtttctg ttaaaacgcc
    42001 gcggcgttct gtttgacgct ttcttcctgt ctgagcaacc aacatgattg ggctgccgat
    42061 acgaccttct aaatctttct tgattgaagc taaagttgtt ggcatacagt tcacctctac
    42121 tatcaactat accacatatt tttaaaattt tcaagtttac cataaaaaaa aaaagaaatg
    42181 caataaaaat aaataagaag ctcattaaga tttttctaaa ttattcctca ttataaaatt
    42241 tcaagtgttt ttttattttg agttttactc tcccgtaagc gctcattact tgcttgtgat
    42301 ccattgctaa acattcagcc atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa
    42361 ttttagcttc tatttttgaa aactggattt cgcaattttc gattgcttct tttaataaaa
    42421 gatgctgaat tgctaaaggt tcttgcaatg tgatctctgc taaaaagtct ggtccttctg
    42481 tctctatttt ttggtcgatt gagacttcat ctttttgatc acgacgcttc agcgcgttct
    42541 gcttcctcaa cagagaatag atttgattgc ttataacgct tttataataa cttccaaagc
    42601 taacaccttt gcttttttcg taagttctca atgcctgata taaagcgatc cttgcttctt
    42661 gttcccaatc ctcagaaaca taatttctta aataataggt gtttttcaaa cgtagtaaaa
    42721 gtggaaaata tttttgatac agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc
    42781 gcaataactc ctcagacata aaataattcc tccataaatt cgtttatgga ggaatcttat
    42841 catcaatgat tcatcgaact gattagaacg atatgttatt tattttttct cattaaatcg
    42901 tccaattttt gggacaattt ttttaactgc tcttcattcc atggagaatt acgtcggaaa
    42961 tcaataaaat gcatatcttt attttcttgg gctattgttt tttccgtttt ttttatcgtc
    43021 ttatataatt cataagcaga agttcgtaat gctccttggg aaaagatcat ccactgttct
    43081 gctaaatcgc ttgtcgccac tgtcacttgt gtcaaacggt cattcagctg accagcgatt
    43141 ctctcgatat aactgtcagc cgtttcgtct tctttcgtaa aaacaacatc caactggtat
    43201 ttcttataat tttgttggat gcccggaact agttgggcat cgaagacaac gatgacttcc
    43261 attccttcat atttcgcata atttgataaa cgatgtaaaa gagcttctct tgcatccgcc
    43321 attttgtcct gctttttaag ctggacaagt tccggccacg aaccaatcat gttgtagccg
    43381 tctacaatca acagctgttt tttcatggtc tactccccct gacgttggga aaaagcctga
    43441 tacataagca aaccagcagc aacactggca tttaagctct ggacgtgccc ggtcatcggg
    43501 atcgtcagca attcatccat ttcttttttc aatcctggac tcattccttt gccttcatta
    43561 ccaatgacta aagcaattga tcctttggca ttccattttt ggaagtttgt gccggtcata
    43621 tctgtgccaa atacccaaaa tcctgtttct ttgagctgtt gcacagcttg taccaaattc
    43681 gtcacgcgtg caacagggac atgttcgact gccccagtgg aagtctttgt tacgataggt
    43741 gtgattccaa ctgctcggtg ttttggtatg atcacaccat cgacgccact tgcgtctgct
    43801 gtccgtaaaa tcgacccaaa attgtgtgga tcttcgatgc tatccaaaat caaaaagaag
    43861 gggtcttctt ttttctcttt tgttcgttca atcagttggt caagggataa atattcataa
    43921 ggtgtaatcg caagaacgac tccttgatgc acgccgtgat cgctcaaagt ttctaatttt
    43981 tgtttaggaa cccatttgac tggcacagct tgttcacgtg cgatttcttt cagttcttct
    44041 actttttctc ctcgtgaatc ttcttgaaga aagagtttat tccctcgccc ttgttgtagc
    44101 gcttctactg cggcatggtg cccaaagacg aaattatctt ctaatgcttc ttcattttga
    44161 tcagaaactg actgatttcg tccagtttgc tttttatttg aatatctatt ttgttttttg
    44221 tcttcaaatc cacgtttcga ttgatgtttg tttgttcttt ctttacccat ttttttcacc
    44281 aactttctga atacaccagc ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg
    44341 cagatagccc atgagcgctt cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt
    44401 ttttgcgctc gtatggcttt tagcatttct tcctctgcga taataaagtt cttcttcttc
    44461 tgtcaaaaga tgttcttcca gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac
    44521 ataattcgtt gccgcacgat gcagcttatt gggttttgtt tctccttgat caatcaggtg
    44581 gtcacgaaca tagatttcgt aaatcgcatc tccaacataa gccaaggcta aaccgttcaa
    44641 ttgtgtgtaa tctctcatgc ttctcttctc catcgggttc cttgcggtgt atcttctaat
    44701 aagatccctt tttctttcaa caaatcacga atttcgtcgc ttcggacaaa atcacgattg
    44761 cttcttgctt gatttcgttc ttcgatcaat tgttcaatct cttcatctaa taactcttct
    44821 gctgtaaata aaataccgaa gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc
    44881 aagacagcgg cagaaacttt cggctggtct agataagtgt tcagccactt agcaagctca
    44941 tagaccactg tgatcccatt tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc
    45001 tgttctaaat tttctaacaa agcgatttta tccgcatcat ctgccaaatt ctctgaggca
    45061 gtttcttggc ggaatttcaa attgtcaaac gtattcttca gtcgttgtaa attggtggct
    45121 gcttctttca tcgtcgtttc actgtagcgg atcggacgac ggtattgtgt ggtcgccatg
    45181 aagaaacgca agacttgtgg atcaacgttt ttgatcattt catgtacagt gatgaaattc
    45241 cctagagatt tactcatttt ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc
    45301 cagtaattag cgaatttttt ccctgttttc gcttcacttt gtgcgatttc attttcatga
    45361 tgaggaaatt ctaaatcttg cccgccacca tggatatcga tcgtttctcc taaatgtttt
    45421 gttgccataa cggaacattc aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc
    45481 caagaaatct cgccaggttt tgcttgtttc cataaagcga aatctaatgg gtcttctttt
    45541 tgctgctgtt cttcccctgt acgctgactt gctccgactt ccagttcatc gatggattgg
    45601 tcacttaatt tcccataatt ttcaaattta cgggtacggt aatacacatc acctgaagat
    45661 tcataagcat agcctttttc aaccaacact tgaataaatg ctaaaatatc tggcatatga
    45721 tccatgactc ttggatgcag tgtagcaggt tcgacattca acgctttcgt atcttcttca
    45781 aatgctcgga tgaaacgatc agcaacttct ggtgctgtaa tacctaattc gtttgctgta
    45841 cggatgattt tatcgtctac atccgtgaaa ttcgatacga aatcgacttt atagcctcga
    45901 tattcaaaat agcgtcgaat tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg
    45961 atataattat acacagtcgg tccgcagaca tacataccga catgtccctt ttgtataggg
    46021 atgaattctt ctttttttct ggtcaatgta ttgtagattt ttatcattct gatctcctca
    46081 ctttttctcc tttgattcga acgacttttg ctggtactcc taccgctgta gcgtcgtctg
    46141 gaacatcttt caacacaacg gcacctgcac cgattttggc gttttttccg atcgttaccg
    46201 gtcctaatat ctgcgcattc gcagagagca tggctccttg tttaacagtt gggtgtcttt
    46261 tacccgtttc ttttcctgtg ccgcctaatg ttacgccatg gaataaaatc acatcgtcct
    46321 cgatttctgc ggtttcgcca atgacgattc ccattccatg gtcgatgaac acgccagttc
    46381 caatcgttgc tcctggatgg atctcaacac cagttaggaa acgccagaat tgagcattga
    46441 tcttcgccaa taaaaagagc cggtgctgat ataaaaaatg agagaaacga tgccaaaaca
    46501 aggcatgaaa accagggtat gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt
    46561 ttctctttgc tgcatcaatc gctcgggtaa accaactcat gactttccac tcctttcgta
    46621 agaattttat gtaggaaatc atcttcttat aaagaaaaga agcgcctttt aaataagttt
    46681 ccttatcaaa agacgctttc cgcgtggttc cacttttctt cataatcaat gattatctct
    46741 gacagctaac gcctgttata cgttcttggc tactttgttt cctataaaac agtttttcct
    46801 gttttctgtg tttcgccaag accactccca gaggcatttc ggcaaccatt cattgatacc
    46861 ttgcaccacc cggtatctct cttgtaatga tcagctgctt acttttttct gttcatcgtg
    46921 tttagttcag tacttgattt aaatgtgcta atgatttttc acgaccaagc aattcgatcg
    46981 tttcaggcaa ttctggacca tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa
    47041 gattttttcc ttttacgcca gtttcttttt gaacagcttt gatggcagct ttgacagttg
    47101 gagcatctac tacttccatt tgttctagct gtgctttgaa tgcgtggaga acagtaggaa
    47161 ctgtttcacc agcaagtact tcttttgctg cttcattcaa tacaggatgt tcattgaaaa
    47221 atagcgtaga cagatcaacg atttctgcag catagctcat ttgaggttga tacaggctaa
    47281 cgatcttctt cacccattcg attgtctttt catctgggtt ttctgctaca cgtccatcag
    47341 cgattagata aggcagacac atgtcagtaa atgcatctag atccattgct ttgatgtaat
    47401 ggttgtttac ccattgcagt tttttcgcat cgaaagctgc gggtgattta cttaggcgtt
    47461 ttggatcgaa cattttgatc aattcttggt gttcgaagat ttcgtcttcg cccacaggtg
    47521 accagcccaa taatgcaata aagttgaaca ttgcatctgg tagatagcct aattcgcgat
    47581 attgttcgat aaattgcaag attgattcgt cacgtttgct tagtttttta cctgtttcac
    47641 tgttgatgat caatgtcata tgtccgaagg ttggtggtgt ccagccaaat gcttcataga
    47701 tcatcagttg ttttggtgta ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt
    47761 tcatcaaatg atcatcgacc gcaacagcaa aattataagt tggcatacca tcgcgttttt
    47821 ggatgacaaa gtcgccaccg atattatcgg attcgaacga aatagcgcct ttcaccatat
    47881 ctgtaaatgc gtattctgta ttgcgaggca cacggaaacg tacaacagat tctagacctt
    47941 gtgcttcttt ttctgcttgt tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag
    48001 gcatttctcc tcttgcgcgt tgtgcttccc gttctgcttc taattcttct tctgtgcaat
    48061 agcatttgta tgctcgattg ctagcaagca gctgatcaat cagcggttga tagatttctt
    48121 tacgttcaga ttgacggtaa gggccgtatt ctccaggatt ttcaggagat tcatcccaat
    48181 tcattcccaa ccaagcaagg ttttccaact ggcttttttc gccatcttcg atattacgtt
    48241 tttggtcagt atcctcaata cgaataataa agtctccatc attgtggcgg gcaaacaaat
    48301 aattgaataa tgcagttctt gcatttccaa tatgcaggtg cccagttgga cttggtgcat
    48361 agcgtacgcg tactttcgtc attttcttcc tcttctttca aactagactt tgactaacag
    48421 caaaaattcc gtcagttacg gcttctcaaa tatcatttat ttaaagccac tcagcacaaa
    48481 ttttaccttt tctaaggtaa atagtaaagc caactggttg aatttcctta ccagttggct
    48541 gaatttcttt tttattttga ttgtttagtc tttttctctg tctctttttt attgtcgatt
    48601 cctcgtcctg catgtgcagg tttagcaaag atcatgcgtc cagcagctgt ttgaagggca
    48661 cttgttacaa cgacttcgat acgttcattc atgtaatgct gtccatcttc tacgacaacc
    48721 atcgtgccgt catcaaggta ggctactcct tgttggcgtt ctgtcccggc ttttactact
    48781 agaacattca tcgtttcacc agggatgacg actggtttta cggcatttgc caacgcattg
    48841 atattaagaa caggtacatt ttggaattca gagactttat tcaagttata gtcattggtt
    48901 acaatgacac catctaaaag cttagccaat ttgatcaatt tgctgtctac ttcactgata
    48961 tcttcaaagt ctccatcgta catttcgaca gatataccct cttctttttg caaggcattt
    49021 aggatatcta atccgcgacg accacgcaca cgtttcaaac tatcccctga gtccgcgatg
    49081 tattgtagtt cgtaaagaac aaaattaggg atcatcaagg ttccttctaa gaatccagtc
    49141 tttgtaatat cataaatccg accgtcaatg atcacgcttg tatccaagat tttatattta
    49201 tggaaatttt ctcctgattt tcgttctaac agttgactct caacttgact agctgtttct
    49261 tgctcgattt ttttgcttcg tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt
    49321 cccattctca atcccagata gcctaagatg atcatcagta aaatcggcaa tacactattg
    49381 acgaatggaa tttccatatt gtaaagaggg gtcgagatca caacacctaa agtcaatccg
    49441 atgatcgcac cgatcgcacc gaagagaaga taagtcaagc tgacctcact tagtttttgt
    49501 tctacttgtt tgataatagc tgagatatgt ttggccaaca gcaaagataa taaaaagaaa
    49561 ataagtgcgc ctaacaggct gttggtaaat gtattattca gccatccgtt tgactgttga
    49621 ccgattgcct gccaagctga gggtaaaaat gaaataccca agcttgcgcc gacaatgatc
    49681 atcagcaaag taatgacacg tttttgcatc gtttttcctc cgtttcgaca atcatttatt
    49741 agttagcgga acacacgctt caacgtttca gccaaagttg ctactccaac tatctcgatt
    49801 ccttttggcg cttcccagtt acctaagttg tttttaggta ggtatacttt tgtgaagccg
    49861 agtttttgga cttctttgac tcgttgttca atatgactga cacgtctgat ttctccagtc
    49921 agtccaattt caccgatgaa acattccgtt ggctgtgttc ctttttcttt atagcttgaa
    49981 gcaatactga cagcgatagc taaatcaatc gctggttcat ttaattttac cccacctgca
    50041 gcttttaagt aagcatcttg attctgaaga agcaacccag ctcttttttc cagcactgcc
    50101 atgatcagag atacccgatt gaaatcaaga cctgttgttg ttcgtttggc atttccgaac
    50161 atagtaggtg tgactaatgc ttggatctct accaaaattg gacgtgtccc ttccattgcc
    50221 acaacgatag cagagccagt tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga
    50281 ttagcgactt cttccaatcc atgctcatgc atttcaaaaa tcccaatctc attcgttgag
    50341 ccaaatcggt tttttactgc tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa
    50401 tataacaccg tatccaccat atgttctaac attcttgggc cggcgatcga gccttctttg
    50461 gtaacatgac cgacgataaa aatggctatc ccatttgttt tagcggtttt gagtagttct
    50521 gcagtggttt ctcttacttg gctgacactt cctgccacac ttgtgatgtc tggctgcgtc
    50581 atcgtttgga tggaatcaat gataacataa tcaggggaga tatgttcgat ggcacgactg
    50641 atttcgttca tatctgtttc agcgtataga taaaattctg tgttgatact tccgaggcgt
    50701 tccgcgcgta attttatctg ctctgcgctt tcttcgccgg atacatataa gacttttccg
    50761 cctatggctg ctaattgctg agaaacttgt aacagcaaag tggatttccc gatacctgga
    50821 tcgccaccaa tcaagaccat agaccctggc accacacctc cgcctaacac acggttcagt
    50881 tcttctaatt ttgttttgat ccgaggttct tttttaggga cgacgtctgc gatttttgtt
    50941 ggttttgctt tttctcctgt caaacttgtc cgagttctgc ggtccgttgt atcttgttcg
    51001 atttcttcga ccatcgtatt ccattttccg caatttgggc agcgtcccaa gaacttaggc
    51061 gacacatacc cgcaattttg gcaaacaaat tgtacttttg atttttttgc cattgtaccg
    51121 cctcctttta tcctaatcat gagacattaa atcaagctta agtatcttta acgttttatt
    51181 tactggaaga accaaatccg ccttgacgtt gagcagtcgt ttgatcttga tctgctttta
    51241 aaaacggcaa aaagatccct tggccgatac gctcgccttt tttgatcact acatccttga
    51301 aaccaaagtt ggtgaattgg aacatgatat ggccttcatt tgcctcatta ttgtaataat
    51361 cactgtcaat cacacctact ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg
    51421 aacggttagc aagctgcaaa aattcatctt ctcccatgta agctttgatt ccagtaggca
    51481 cgagggttgg tttcaattgt ttttcatttt ctgatacatc ttttttcaat ccatctgcga
    51541 ctgtttccaa cccctttttc cagcctgctt tccagatact tggaatcacg acctcttctg
    51601 ctgcttcaaa atcatagcca gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt
    51661 gatctttata tttagaaata acttcaaatc ctctttttct caccggtttt cctccctaaa
    51721 taaactactc ctattctaac atatccgagg ctgtaaaaga ctttctttac aaattcttca
    51781 agaaattctg ctatcttaaa gtaaaccatt aattcgttct ttttccaaac gactgcaagt
    51841 tgtacaaaaa aactattttt tacatgactt tactcgtcaa aacaagtaga atagaacgaa
    51901 attaatgagt ggatatttga ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac
    51961 gaattattgt caaagtagga acaagtacat tgatttatcc caatggaaat atcaatcttg
    52021 cggcaatcga tcagttagct tttgtccttt ctgatcttag aaatcgggga aaggaagtga
    52081 tcttagtctc atcaggagct gtaggcgttg gtatgcataa attacatttg tcagaacgtc
    52141 cgactttgat tcctgaacag caagctgcgg cggctgttgg tcaagccgaa ctgatgaata
    52201 tctataatca aagattcagt gtatataatc aacagacggc acagatcctc gtgacaagag
    52261 acgtggtaga ttatcctgaa agtcgtaaaa atgtgaggaa tacattggaa cagcttttga
    52321 ttatggacac gatccctatc gtgaacgaaa acgatactgt agcagtggat gaattagatc
    52381 atctgacaaa atttggcgat aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg
    52441 cagacctttt gatcgtcctt tctgatatcg acgggtttta ttcagataat ccgactacaa
    52501 atcccgaggc tcagatgtat cgctatatcg ataaaatcga tgaaaagatt ctggcacaag
    52561 caggtggtgc tggtagcaca tttggaacag gcggtatgca gagtaaatta aaagccgctt
    52621 ctaggatatt tgaagcaaac agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta
    52681 tttttgatat tcttgcagga aaagaagtag gtactttatt taaggaggac ttgaaatgac
    52741 tgacttgatg caattaggac aacaggcaaa agatagcgcg caacaactag cacttatgcc
    52801 tacaaaccgc aaaaatgagc tacttgttca aatggcacaa accatcaaaa agaatcaaca
    52861 agcgatcatt gaagcaaatc ataaagactt agaaaaagca gtcgaaaaca atatcagtga
    52921 aacgatgcaa gaccgcttgc agctgacacc tgagcgaatc aatgccatgg ctacagagat
    52981 cgaaaaaatt gcttcgttag acgatcctat tggaaaagta gatgagatgt ggacgaacac
    53041 cgacggattg agaatcggca aaaagcgcgt gccactaggc gtgatcggca tcatttacga
    53101 atctcgtcca aatgtcacga cagatgcagc tagtctagca ttcaaatcag ggaatgccgt
    53161 tatccttcgc ggtggcaaag aagcattctt ctcgaatcaa ttattagtcc aattattgca
    53221 gcaagtactg gtcagtgctg gcgaatctcc ttatgccatc cagtttgtcg atgatacttc
    53281 tcatgaaact gcaggacagt tgatgcgatt gacagaatac ttagatgtgc tgattcctcg
    53341 aggcggagca aaattaatcc aacgagtcaa agaacaggca acggtaccta tcattgaaac
    53401 aggtacagga aataatcacg tatatatcga taaagaagca cagttatcta tggcagtgaa
    53461 catcatcgtc aatgcaaaag cttcccgtcc atctgtctgt aacgcggcag aaacattgct
    53521 gatccattct gagatcgcac cttttttctt gccagctatt gaaaaagaac tggtagaaca
    53581 cggggtctct ttacgtgcgg atgctcgtgc gttagaatat ctagagacgg ctgctttagc
    53641 agaagacaca gattgggata ctgaatatct agattatatc cttgctgtca aagtggtcga
    53701 ttctttggat gaagcaattg cacatatcaa tcggcataat actaaacatt cagaaacgat
    53761 cgtgacagat agttatgcgg ctagtcaacg tttcttaaat gaagtggacg cggcggctgt
    53821 ctatgtcaat gcctccactc gttttacaga cggatcggta tttggttttg gtgcagagat
    53881 tggtatctct actcaaaagt tgcatgcccg tggtccaatg gggttaaatg aattaacttc
    53941 cacaaaatat atcatttatg gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat
    54001 tattcccgcc ttttgccttt tttctgtaaa ggttctatcc aactagaaag gtgtcaatat
    54061 gtttgaaatt ttaatgattt tttttattta ccttatttct ctgaatatcg ctgcttttct
    54121 gggagtcggt attttatccc ttttctttca attcaaaaaa cgaagcatag ggagtcaacg
    54181 ggaaaaatgg tcgcagtatt ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca
    54241 cattagttac atggttgcat tatgcttatt agcgacaatc aattactatt cttttttcga
    54301 tcattcaatc gcttatacga ttacgctact gattgctggg atttttcact tatcttataa
    54361 atatcagctg aataaaaacc acttgaatcg gactttccgc taaaaaatat ccgtacgata
    54421 agtagagtgc cttgaagatc ggcaaaaaac tgccggactt tttagaggct gtgacaaaga
    54481 ttttgtcaca gcctcttttt ccatataaac ggcgttcgcc taccagctgc ctggtaaatc
    54541 tgtttcctct tcgttaagaa gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag
    54601 cagctcatcc atggacaagt tcatcgtttt ttctttttct tcttccgctg attcagccag
    54661 tagctgttcc cgatacacct gatacatacc agctattttt tgctgcatcg cttttctagt
    54721 ttcaccttcc gcattgatta ttggcaattc tttgatatag ccacgataaa tcaaccgatc
    54781 atctgaaaat tcaatgggta attcaacgag tgtcaacccg acttcctgaa aatattcttc
    54841 cagcatctat tatcccccca ttctttttct taaatgaatt ttagcttacc tactgaaaat
    54901 tttccagttt cattattttg agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc
    54961 tttttccgaa taaatggtgt tcttctgcca actcctcaat cataagtcac aatattcaac
    55021 aaacatgaga aactgttttt tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc
    55081 acaacctctt ggtaaacggc gttcaatcag ctgaccctcg gtattagctc aaaaaacggt
    55141 aaaatatgag aagctatttt tccgttttgt ttctctaata cttaggataa aacgctgatt
    55201 ttacaacctc tgttaaacgg tgttcaatca gctgtccttc ggtattagct caaaaaacgg
    55261 caaaatatga ggaactattt ttccgtttgt ttctctaata ctcaggacaa aacgctgatt
    55321 ttacaacctc ctgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa
    55381 acgaaaaata tgagaagcta tttttgtttg gctgttctta tgacttgatg caggacagct
    55441 ttttcacaac ctctgatttc ttattcttct tcatttgtag caaaataatt acggtgataa
    55501 atgatacaac atacactgat aagaacaatg gcacctgcaa aataaaacgc atagctttca
    55561 ccaatatgtt caccaatcac acctgcaata aatggtgcta aagctccacc gaaaaagcgg
    55621 acaaaactat agctggacga agcaacgttt cgttgcagac ctggtatttc catagcaatc
    55681 gtcgtcaaca gtgtattgat aattccttgg aaaaatcctg ctaacacaat gctgatcggt
    55741 acaaggactg gcatagaagt tccagctcct aataacagca aacaaataat gaataagaca
    55801 aatgctgcta aaattgtccg ataagtcgta aacttccgct caagagccgg tgcaacgaaa
    55861 atcgaagcaa tagccagaag cacaccccag ccaaaaaata caaaaccgac ttctaattca
    55921 gaatagccaa tcaataaaaa tggcgcatac gcaagtaaag taaagaatcc gaaattatat
    55981 aacaatgcga ttagtccaac tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc
    56041 caaaggccta cttttctctt tggtttctca gtttcaggta aaagtaccgc tagggctata
    56101 aaaccgcaaa acatcaaaaa tgctacccca aaaaatggat aacgccatga accactgccc
    56161 aagaccccac cgactagagg gccaacagcc attccgcagc caagtgctgc ttcatacatc
    56221 atgattgctt tttctgtgtt accaactaaa atactgacga tcgttgctaa agcagtagag
    56281 acaaataaag cattcccgac accccagccg gcacgtaacc caatcagcat tccgatattc
    56341 ccagataatc cgccaagacc agcgaataca acgatgataa acaatcctgt catcaatgtt
    56401 tttttcaaac cgatccgact agataagaaa cctgtaaata gcatcaccac accagtgact
    56461 aacatgtaac tagtaaataa cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca
    56521 atcgttttta agattgggtc tactagaccc actcccataa aagcgatcat acaggtagct
    56581 gctacaacta gcgctgaact tttccccttt gtttcttcat ttgtttgttc ttgtatatct
    56641 atttgattct ccaatatttc catccctttc tctctattag catcatacat gatttatttt
    56701 acatgtcaat atgcacatgt aaaacgtgga aactttactg tgatataata aagaaaataa
    56761 gacgagaggt aggattttta tgaatacttt agcctatata ttattatgcg tgcttttatc
    56821 caaaccacgt tctgggtatg aattgaagca gctgatcaca attttttggg aagcccatca
    56881 cagtcagatc tatacaacgc ttgccaagtt agaccagcag ggttatgtga ccgttttgga
    56941 caaagacgag cactctcaaa aaaagatcta ccacttgact gatgaaggac gtgctcttgt
    57001 agaggaatgg atcaaagagg aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa
    57061 aatctatgct ttttcgacgc tcgataaaac aacagctact ggactgctat tcactagaga
    57121 acaacagctg aacaaaatca tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga
    57181 caagaaacaa gaatttggac gttatgttgt gatccagcgc agaattatgc tttgtcagca
    57241 ggaactccaa tggtgtcagc aagtaagaga acaaatgcag cagttttttg agtaagggat
    57301 aaacagaacc tttctagaaa tatctattga agagcaaatt atcttcaata aaagagcaat
    57361 acagttggaa aagaaaaagc gatccgaacg acaggccaaa acctatcgtt cggatcacta
    57421 tttttctaga actgcattca agagactatg acaaaaagca tgccaaggcc ttttttcaaa
    57481 taacagtgtt ccatcattca ttactttgaa ttgcttttag cacacttctt gaaagcgggc
    57541 caacgatgat caactgcaac ggtaaagcgg caatcacatt cattttccaa gcattcatat
    57601 aagcagagaa taattgatca ggtattcctt gttccatgat cacaccgaat aaagacatac
    57661 atgtcaccat tcctaaaacc atcaagcatg aaatagttaa gatcatttgg accttgcttt
    57721 ctggatttac tggtagttta aaggcaattt ttttcgccag cacaccgatg atgaagacat
    57781 ccaaaatgaa agccaccacg aatccaggca ctagaccaat caacaaactt gataaatgaa
    57841 gatcttgatg cagccataaa ttatacgcgc tcatccccaa gaccatcaaa aaacacatca
    57901 ttgttgtaaa aattattcct tcttttttat ttgtaggcaa tctgtaccac tcctttaaat
    57961 ttgtcacgaa aatcatcgta gcacatttaa aaatttctcg taagtacttt ttttattttc
    58021 tgccctttta tttcataaac ttcatcagcc gtacgctcga caaagtaaga atcatgagaa
    58081 gtaaacaaaa tcgttcccgg atatatttgc attagcttct ctaaagcttc gatcgttgcc
    58141 atatcaataa aattagtcgg ttcatctaac agcaacacat tacttggctt agtaaaaagc
    58201 aaagcaatcg tcagacgggt cgcttctccc ccacttaatg tacataacgg tttcgtcaaa
    58261 gtttcctgag caaaccctaa gtgattcagc aaactacgga caagcgattc tgaaaactct
    58321 gtttgtctca tgacaaaacg aatgatggat tcttcagatg tcatttgata acccttttgc
    58381 tgatatactt gaaagcttac tttcggagag agaaggattc cttctccttg tttgcggata
    58441 tgttctaata aggttgtctt acctgaacca ttttcaccga caatcgcgat ttttttacct
    58501 aaagaaaact gaaaatcacc atttaccagt aatgttctcc cacttctttc caattggaca
    58561 ttttgtccca tgatgggata gcggctgtgg agttcaagag attttggtac tggaaaacgg
    58621 atttgtttca tctgctgcgg cttggttgtt tctcctattt tttggagtct tctctctaat
    58681 gttttcgcat tcttttggat ggctttttgt acactgtctt tttgtttaga ggaagacaac
    58741 cgatccagtc tgatttgttg ctttttcttt ttactggaca ctttttccaa cttttccgct
    58801 tgttttttct tttcttggat cgatttttcc aatcgttttt tctctttcat gatcgactct
    58861 gcttctcgta gctgagtctt tttctccaat tccttttgag tgagataggc actataattc
    58921 cccgaaaact ctcggatttc tccgtctttt acttcccaga ttttcgatgc tagctcatct
    58981 agaaaatagc gatcatgact aacaaaaacc agcgtaccat aataataacg aagctcttca
    59041 atcagtcgat caatactttt tctatctaga tgggttgttg gctcgtctag aagcatccct
    59101 tgttcataag aagaaagaag acgtatcaac tcgatctttc gttcttcccc tccgctccat
    59161 ttccgctctg aagtttcttc cagtcttaac tggaaatgac tggctaattc caaatttagg
    59221 tcctttgctt cagcgagata ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg
    59281 attttcccat gatcgggaaa aagttcaccg gctattagtt tgagcaaagt cgatttaccg
    59341 acaccatttt ctcctatgat ccctatacga tcttgctgat agacacgaag agaatcaata
    59401 gatagttcta gctggttgcc aaagctgact tgtagatttg ttatatttac tgctaaattt
    59461 tccatatgtt ttacactcct atttcatgta aaacaacaat tatcgatact aaaaaagagc
    59521 agaaaaatct gtttgcaaaa aaactgcatc acatcttttc ctactctgaa cgaaataact
    59581 atgactaaat caccaaatct gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg
    59641 gacatactcc atccatgaaa ttgttgttta tacatgtctt ttgaatttat ttagttgtta
    59701 ttcaaaagtt cgaaacgtaa tttcatcgat gcagtcattt ttccgacctc cttttttatt
    59761 aggttaccac acacgattca aaaaaagcaa tagctactga atagtaactg aaaacctttt
    59821 attcgaacat agtgatcttt tctggtgcta aacgaacaga ctcgtaacct tcttctaccg
    59881 cttttcctat tgataaaata ataactggaa cataacggtc tttatccaaa ccaaatgcct
    59941 ctgccaactg atctgcttcg aatccaccaa ttggatttgt ttcatagcca tgatcacgcg
    60001 caactaacat gaattgcata gccgctaagc tagaatcgac tttgactaca tcgttcattt
    60061 cttctcttga aaaactcttg taataaggaa tgatcgcagc taattgttga tctctgactt
    60121 cttttggcat cttccctgat tcgtaagctt ggttgtagat ttcttcgcca tattcgtaac
    60181 attgcatatc gccaaaaatc atgaccattg cagaagaagt atcattttgt ctcgtattga
    60241 aacggatcaa aggacgcaag atttcttttg cttcctcgct ttcagcgacc acaaagcgcc
    60301 atggtttcat attgacagaa gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt
    60361 cttcgtgtgg gattttgtat gtttcatcat atacgcgaac ggatcttcta ccagtcataa
    60421 ttgttgaaaa atcatttttt tgttccatta ttattaacct ctttcgtttc tgttgttata
    60481 ttgcaaagtt gggtattcaa tttatctaaa agtgttagaa attgtttcat ttctacatcg
    60541 ctgaaagccg gggatagcac ggaacctgta tctttatggt ccatttcaca atgcttgatt
    60601 ttcttctggg ctttttctgt tagcgctaca tacacttccc ggttgttctg aggatttctc
    60661 tgacgcgaaa catagccatt tgcttccaat accttcagat gacgagtaac tgctccttga
    60721 tcaatctgca acttttcttg caaactgctt tgtagcaatg gattttcttc tttcagtctc
    60781 atcatgatct cgtatctagt aagacttata ccgatatttt cttcaaagat ctggacgatt
    60841 ttttgatcgg ctaatttgat ttgatataaa aggtgactaa tctcttctaa agacataatt
    60901 tcctcctttc taataattga ctcgtcaacg attgacttgt caataataga ataaaaaaag
    60961 aaaaaagtca aagaatcagc tgtgtaataa aaaaaacgct ctcaatagaa aagtatagag
    61021 aaaagaaatt ttttgtagaa tgaacggaaa aaatgataaa gaaagcacaa cgctaaaacc
    61081 aagattattg attcttatca aactatagaa gatagctctt ctgcagtaga taattttaca
    61141 ccgccaaaaa tttccgaaat cgccttttgc aagaatcccg tcgaatatta gaaattggat
    61201 cagtatgctt aaacagtaat aaaaaagtta tgacaagatg ttgtcacaac cttttttgag
    61261 taaacggtgt tcaaaaactt gcatctgcgg taataagttc aaccaaacaa aaaacatgaa
    61321 aatctgtttt tgtttggctg ttcttactac ttgatgcagg gcagcttttt cactacctct
    61381 ttttaataac cataaaatcc tattccttca tatctccagc ccatcggtgt tagccaatct
    61441 ctttccttag cactcaaggt atagtgatgg gtaccagttt tttcatttgg attaaatagt
    61501 cgataaattg gcttggaacc accagaataa aaacttagac cttcataacg ccaaccgatt
    61561 tttgttaaag agttgatctc agattgtgcc attgtgtaat gatggtctcc actgtaagga
    61621 ttgtataaac ggtagacagg ttgtccgctg tttggtgctt gccaagcaat cccttcatat
    61681 tcccagccta cagttggcag ataatttttt tctccggcat tcaatgtata taaatgttct
    61741 cctgtatgac ggttatacag acgatacatc ggagaagttg aagttggtgc tgtatcatac
    61801 tgtgtcagat tataggtttc gattacattg ttcaatgtag aaccataatt aggcgcagtg
    61861 gcataacgac ctgttagcca agccgtagca tctttgtaag agttcgtatt gcttttccat
    61921 gcaccggaat agtagtaaac gcctggctga aaagaagtcg tcttcaatac cgttacgtta
    61981 tcttgcaatg attctttgta cgaaggatat tttctaaaag cggcattcac cgtaatccat
    62041 tgcccattca catattccga agtaggcatc gtaacagatt ggccattata gcttccttta
    62101 atcccaaata gattgtaatt aggggcagaa gccaaagtac tgttccccca gccactttca
    62161 acgatggctt gagcaatcat gactgatgca tataagtcat tggcacttgc caaactttgt
    62221 gcagttgagg caatactatc aatgaacgct tgttgagaat tagcagatga gcgagcataa
    62281 aaagcatagc cagtatagtt ttcgaattca gacaaatctg cttgatgggt atgtgtcaat
    62341 gtttgcccat ctgtcaattg gttcgtgatt tcttctgcat tctctggatt gttctgagct
    62401 tctgctgatg tactgtcttc ggtttgtgtt tgttctgttg tttcagttgt tggttcttcg
    62461 gttgaaattg tattttctga gacagtagct gactgcaaag tactttctgt atcgttttca
    62521 attcctgttt ctgaagaact cgatgtctga gttgcgacag tttctgtcga ttcttcagct
    62581 agcactgttg tgttggcaac tgctagattt cctaaaagaa taacgctaaa taataaattc
    62641 tttgctttca tcttttttct cctttgaatt atgaaacttt tcctaaataa cagtgtaact
    62701 aaaaaagtaa aagcaagtca atgtccatat ttaaataaca tttgtttctt tttaataaat
    62761 ttgctttttc tgcataaaaa gacgtaaatg aagaatattc ttctccattc acgccttaaa
    62821 ttgatggtcg tttgaataac tatttaacaa gtaattctct ctggatatta tccgttatgg
    62881 attatacttc ctgtgtcgtc ggacggataa tcatgttgct gacagacatc cggtctggcg
    62941 tttctatcgc aaatgctaca gcagaagcta tatcttcaga tgttaatccc cattcttttt
    63001 gcgcttcatg aagttcaaga gcagcctttt tgtcgctgat ggtttgatat aattcagttt
    63061 gaacagctcc tggagagatg atcgtagatt tgatgttatt ttgccgttgc tcttgacgta
    63121 atccttccat gattgcacgg acagcaaatt ttgttccgca ataaacggcg gaatcaggat
    63181 aaacaacatg tcccgccaca gaatcagttg aaatgatatg cccagatttt tgtttttcca
    63241 tgattggaag tacggcggaa attccattta acacacccat gatgttgata tcaagcatat
    63301 tttgccattc atcacggtga ccttcaacta atggagcggt tggcatgatt cctgcattat
    63361 tgtacaatac atcgattcga ccgaactttt ccatcgctaa atcgataact gcttggactt
    63421 gtgcaaaatc tcttacatct gctgtttgaa tatatagctc agcttctggt aaagattctt
    63481 ttattgcttt taggcgatct tctcggcgtg cagctataac tagtttagcc cctttttctg
    63541 ctaatagctt cgttgttgct tcaccgattc cgcttgaagc gcccatgatg acaatcactt
    63601 tttctgataa atcattcatt ctattctcct cctgttttga aaaatctctt gcttatactc
    63661 actatctctt atttcatctt actgttcaaa taatccgttc gacaggtttg acgattttgg
    63721 cagggattcc tgcaacgatg gtattgtctg ggacatcttt cgtaacagtt gcattcgctg
    63781 caacaatggc attctcgcca atcgttacgc ctggcagaat agttgctcct gctccgatcc
    63841 aagcattttt cttgatgtgg attggcttta ctgacaagcc tcttcgttct tttggatcgg
    63901 ttagatggtt caccgtgatg aggttgacac gaggaccaat caatactgaa tcttctaaac
    63961 agatgccacc taagtcaacg aacatcactt gacgattgat aaagacattt ttccctatac
    64021 taatatgttt gccgaaatct gtataaaaag gcagcagaac agtcaccgta tcatctactt
    64081 tgtcactaat gatattttct agccgtttgc ggatatctgc tggttcatgg tacccagtat
    64141 tcagcgctgc taaaggctct tcgttttccg cttggatctg atggatcact tcaaagagcg
    64201 gatccgttac tcgaatctct ttattgacga tttgtttttc gatgaggctt gtcattttgc
    64261 tccctccttt tatccttact ttataacgga cggcttatga tgtctaatac ttataaaaca
    64321 tacttaacta ttccttttaa ctatacttag tggtatgctt ttttaaaaac aggagggaaa
    64381 aaatggatat tcgtatactg acgtattttt tagcagtagc tagagaaaaa aatatcagcc
    64441 gagcagcaaa atctctgcat atcacgcaac ctactctctc aaaacaactg aaagatttag
    64501 aagaagaact tggtgtgaca cttttcactc gaggctcccg agagatacag ttgacggatt
    64561 ctggtcgata tctttattca aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga
    64621 acaatttaac aaatgacgca atcatctctg gagaaatcta cattggcgga ggcgaaacta
    64681 aagcaatctc tttcatttct gatagccttc attcgttgat caaggcgcat cctgatatcc
    64741 agtttcattt atatagcggg aatgcagatg atatcataga caaactggat agcggattac
    64801 ttgattttgg actcgtaatc gaaccgacaa acaaacaaaa ataccagtca tttcgactgc
    64861 cagcccaaga tcgttgggga cttttgatac atcggaatca tcctttagca aagcaaacag
    64921 tcatccatcc gcaggaactg aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg
    64981 acagtcaact agctgaatgg ctaggtttca gcatagatca gttatcgatc gtcggaacat
    65041 ataacttatt gtacaatgct tctttgatgg tcgataacgg gacttttggt gcattagctc
    65101 tagatgggat catcaatact tatgggacgg aaaaagtatt catcccactc gatccgccat
    65161 tacaagcttc actgaatctc atttggaaaa aagaccagcc tctttctcct gctgctcgta
    65221 aatttttaga tgtcctaacg gagaaataca gttaagtaga gtttttttcc ttcgctttca
    65281 gctttttaac aacgaataag ttaagcaaaa gatccaatga ttaaataaca taagtttctt
    65341 aatttgttcc ttccttttat ttctgataat tccagtaaat tactataatt ataaaaaaag
    65401 aattgacaaa acataactgt ctatataaaa ttgattacgt taacaataat tcgtttcatg
    65461 tgactagatc atactaggca tgaaaaatct ctgaggaaca tggtttccct aggaggtttt
    65521 tcgtgtctat ttttgtttgg ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt
    65581 caaaaagggt gcgcgcatga aaatcaaaaa gatctttaat caaaatgcag tattggtaga
    65641 tgacggtgga caggaaaaag tagctattgg aaaagggatt ggatttgaca gaaaaagaaa
    65701 tgatttgatt tttgatcgtg atgtcgaacg gatatttgtc atggaaccag aaggtcaaat
    65761 caagttgcaa aatttactta atcagattga tgaacgcttt ttatttgcag cagaaacgat
    65821 cattgatcat gcggaaactg ttttgatgga aaagttgaat gaacatgtat tgatcgcttt
    65881 gaccgaccac attgcttttt ctgcggagaa cattaataac ggtatcgtca tccgcaacaa
    65941 acttttaaga gaaatcgaag tgctttacag tgatgaattc agtattgctc aatgggcagt
    66001 agagtatctg acaaaagagc ttgggattcc atatacttat gacgaagcag gatatatcgc
    66061 aatccatatt cacagcggac gaagcggtcg gaacaataac catcgcagta tccgtgaagt
    66121 gacgattgta tctgacatca tccatttagt ggaatcagaa ttggaaacag atatccattc
    66181 agaaccattt tcactgaatt attcaagact ggtgaatcat ttacgactgc ttctacaacg
    66241 aacccatgcg caacaatatg ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa
    66301 atacccagaa agctataaga tttctaaaaa aatccgtgtg ttgctgacca aagaatacca
    66361 gctcgcgatc acgaaagaag aactgggtta cttggccatc catatcgagc gtttaagaag
    66421 cgcaatcgta caaacaaatg ttaacaatca tgaggaggaa aaaaattgaa agcatatcta
    66481 caacgaatgg gacgttccct tcaacttcca gttgcggtct tacctgctgc tgctttacta
    66541 gtagggatcg gaaactggtg ggcatcttat agtaacgaca ttgtcgctca ttttttacaa
    66601 gctggcggta acgcagtttt aggtcaatta cctttgctgt ttgccgttgg gttagcacta
    66661 gggatgtcag tggacaaatc aggtgctgct gccttggcag gtttggtcgc atttgaagtt
    66721 ccagtaaatg ttttaaaaac tgattcagta gctactttat tgaatatcaa accagaatca
    66781 gttgatccat cttttgctca gatcagtaac gtatttattg gtattattgc cggattgatt
    66841 gcggcagctc tttataatcg tttccacgaa gcaaaattac cgatggcttt atctttcttt
    66901 agcggcaagc gtctagtgcc aatcttggct gcctttgtca tgttgctgat cagtgcagtg
    66961 ctactgctcg tatggcctcc ggtttactct gctttagttt cttttgctaa attcattttg
    67021 agtttaggct ggggtggtgc aggtctttat ggcttcttca accgtttact gatccctaca
    67081 ggattgcatc aagcaatgaa ctccgtgttc tggtttgacg tagcaggaat caatgatatt
    67141 ggtaaatttt tggcttctga aggaacaaaa ggcgtaacag gaatgtatca agctggattt
    67201 ttcccaatca tgatgttcgg gcttccagca ggagcatatg ctatttacag aaatgcacgt
    67261 ccagaaaaca aagcaaaaac agcttccttg atgttagcag cagcttttgc ttcattcttt
    67321 actggtgtaa cagaaccact tgaattctca tttatgttcg ttgcttggcc attatatgtc
    67381 ttgcatgcaa ttttcactgg gatttcttta gcagtgagtg cattcttcca ttggacagca
    67441 ggatttgcct ttagtgccgg atttgtcgat tacttcctat ctttgaaaaa tccgattgcg
    67501 aatcagccat tgatgttgat cgtccaagga ttagtaacag cagtgattta ctactttggc
    67561 tttaactttg ctatcaaaaa attccatctg atgactcctg gccgcgagga agcagatctt
    67621 tcagatgacg atactgcaac gactaattca gacaataaat atgcggcaca agcaagcaaa
    67681 atctacgctg cattaggcgg agccgacaat gtaacttcga tcgataactg tactacacgc
    67741 cttcgtttgc aagtcaaaga tacaggcctg atcgatcaaa ataaaatcaa agccactggc
    67801 gtacctggta tgaagatcat cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta
    67861 caattcgttg cagatgagat ggcaaaacta catggtggtg ctgcaacacg acctgcacaa
    67921 acaaacaccg tggttaaaac agaaacattt gccggtggaa cgatcgaaca agatatttac
    67981 gctatcgcaa atgggaaact gattccaatc actgaagttt cagatgatgt attcgcggaa
    68041 aaaatgatgg gtgacggcta tgctgtatta ccagaaaatg gagaaatctt ctctccaatc
    68101 gctggtacga tcacaaacat tttcccaacg aaacatgctg tcggaatcca aacagatgct
    68161 ggtatcgaag tcttgcttca tatgggcatc aacactgttg acttaaaagg cgaaccattc
    68221 actttatatg taaaagaagg tcaaaaagtc gcaagaggtc aattgattgc tttagttgat
    68281 cttgccgcta ttcaatctgc aggcaaaaac acggatatgg tcgttgtatt cacaaatggc
    68341 gacaaagtcc aaagcttaga aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc
    68401 ggctctgttt ccaacaaagc ctgagccatc gtcttcccca gactttttta tcgataaaca
    68461 aacaggagct gagacaaaaa tcttgtctca gctccttttt ccgaataaaa acggcgttct
    68521 cctgccaact ccgcggtaat aagtccagcc aaacgtaaca tatgaagagc tattttttat
    68581 ttgactcttc ttattacttg atgcagaaca gcttttttac aacctctgat aaacggtgtt
    68641 caaaaaccag tttatcggta tgaagtcaaa tttatgcaaa aatgtggaaa gccattttca
    68701 caaaattttt cttcatactc aaaactaatc ggctttttca caacctcttt atacattatt
    68761 cctgatccaa tatttctttt aatgctgctt caagagaagt taatggtttt cccaatactt
    68821 tttctaaatc atcagaggaa atagctaact gatccttttt aatatcatgt tggatcgcta
    68881 aaaacatgtc agctgcttct tgagggaatc cggcttcttt tagattttcg acaaacgctg
    68941 catcacttgc ttcaataatt tctaactttt tacctgtcgc acgctctaat gccttggcta
    69001 attcttcata gtcagttaga ttgccggata attctaagat ttctggagag tcactttcta
    69061 ctagtacttt tgctgcagct tctgcatatt cacgtttcaa tgcccaacct gtttttccat
    69121 ttcctgcagc ataaacgaat tgaccagttt ttaaagcctg cccaatgata ggtaattcat
    69181 tctctacata ccaattattc cgtaaaaatg tatgggaaat acctgaatcc ttaatcattt
    69241 tttcagtgaa ttggtgatct ggcgctaata cactgatcga gtgatcggca tctgcaaaac
    69301 ttgtataagc gatataagat accccagctg cttgtgctgc cttcactaca ttcttatgtt
    69361 cggcttgacg atttcctgga acgccagaga caaaaagcaa tcgatctatt cctttcaacg
    69421 cttctttcat tgagtctaaa tcatcataat caccaatacg tatattaaat ccagcttctt
    69481 tcagtgcttc tcctttttct tcactacgta ctagtacata gatttcattt tgtggaacca
    69541 tcttttttaa gaattccagc gcataaccac caaaacctcc agttgctcct gttactaaat
    69601 aatgcatatt tgccactccc tattcttttt atttgttaag atttacagct gtacatttat
    69661 aaattacacc tttatcataa agagtgtggt aagataagtc aagcaaaaaa aatgaaagga
    69721 tgacaactga tgaagtattc tctgcaattt agtgatgcaa tccatgtact tgcctatatt
    69781 gaaatgtttc aagatacccg atcactttct agtgagatga ttgccagtag tatcgaaaca
    69841 aatgcagcaa atgtacgtaa aatcatgagt cagctcaaaa aatccggttt gatctttaca
    69901 aaaaccggaa aaccgcagcc ttcccttgcc aaaagtccag atcagatttc tttactagat
    69961 gtctatcgca gcatcgaagg taatacacag cttatccaag tcgatccaaa aacaaatcca
    70021 aattgcgtcg ttggtgcgaa tatccaagaa gctttagaag aaagctacgc tcggctgcag
    70081 aaaaaggcag aagaagaaat ggctaagatc acacttgata gtctgataca taaaatagcc
    70141 gttttggaaa aagaaaaaag accagaaaac acaggcctcg tagagagatt tttataattc
    70201 tctctactgt gcctgcattt ttctgatcgg aatcgtctac ataaattctt ttattctccg
    70261 ggggacaaaa gccttgtcat gccccttttt cctctctaat gctcaggtca aagcgctgaa
    70321 gtcatgacgt cttgataaac ggtgttcaag tagctgaccc tcggcattag ttctgaaaaa
    70381 tgcaaaatat ggaaagctat ttcccatttt tcctctctaa tgctcaggtc aaagcgctac
    70441 tttcacgacc tctatattat catggtttat tgactaccgg tatccagatt tctcccatat
    70501 atttcccgtt ggtaactccc atttcgacta cagcatttgg acccccaaca taggcgtaat
    70561 cttctacttc tcttaaaaca tgcccaaagg ccgtattcgt caaatcattt cttaattctt
    70621 caggcgattc tgctaaagcg attacaacaa gatacgttcc ttttgggaat gggatgattc
    70681 gggtagtgtt tggaacatcc tgactcgttt gaacaccgac atagtacatc attttcccgt
    70741 tatagggttc gttgactgta aagaaataat gattttcagc agcttgtttc aatgcatcta
    70801 ttgttccatt accgataact ttttgctcaa atgcttgttt ctctgcagcc aaaccgacaa
    70861 aatccgtatt gctactcttc aattccacac caatacccgt caccgtaaaa tctggttttt
    70921 cttcgatcct atactttgcc atttctccca ccctttctat tatattaagt tcatcttaca
    70981 taaaattaaa gggaaatgac acgattacgc tcttttttct agggatattt ttatttttta
    71041 actatttttt tgctttcact gcaatccaaa tctcactgta aacattactg aaatcctctc
    71101 cccattttgt aaaagagatc tcgggagctt cgactaactc ataatctgaa gaaggaagcc
    71161 attctgcata gattttcccc caagtatctt gtaaagtttg gggaaatggc cctttattag
    71221 gaaaaatggc ccaagtgtgt tcttctatag caagcatttc taaatcagcg tacggatttt
    71281 cttttgtcgt agcaaagcca atcatgtgca tcaaactgcc cttttcttcc aagcgggctc
    71341 catcaaatcc ataagaaacg ttgattactt gatttggata taaatctcct aacttatgca
    71401 tctcttccct ttgagtctcc gtgatcgtct gcgctaactt ttgtatttct tgattgactc
    71461 cttcaaattt gacaggaact tttttcgcca cacctgccac ataaaactgt tctttctttt
    71521 cgatacgaaa ttccatcgat tttcctcctc tgacatcaat aaaaaatgtc aagcgtggaa
    71581 aagatttttg tatgccgtat ctgctgattt ctgaaggcag atatcctgac caatcacgaa
    71641 aagctcttga aaaaccttcg actgtttcat atccatatct aaacgccgtt tctgtcactc
    71701 ttgatccatc taggagatcg cgattagcca aagccaatct acggtttttg atatagtcca
    71761 ttagcgacat ccctgcaata aacgtaaaga tgcgttttaa ttgatattca gagattcctg
    71821 tattttcggc tattatttta taggaaagtc cttctgtcaa atgttcttcg atgtaatcga
    71881 tcaattggtt cagatcttta agcatattta tcttcctcct tgcttgcctt tttatcatat
    71941 tatcaaagaa agtcaattac ttgatgaatc aggaaccaat ttagtcagat gtagaaaatg
    72001 ttgtatggat ctaaagaaaa ttctcaagca gcgtttcgta acggataatt ggtattatct
    72061 gcctatctat gttttacctt tagaaaaata aattttgggg aagacacatt ctaagtaaac
    72121 aaagcggtaa aaatatcttt tattccctta gttaatccgc ttactaccta ctgtacaaag
    72181 caaattgttg ttcaaactgc tgatcagaac gctgcttgga tttttctttg cgatacatca
    72241 tacctctttt atcaattgga acaattattt atttctaaca aaatcacttc atttttgtca
    72301 gataaataaa a
//
LOCUS       DACECJ010000003        71080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.003, whole genome shotgun sequence.
ACCESSION   DACECJ010000003 DACECJ010000000
VERSION     DACECJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.003"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IWO09_000167"
     CDS             161..391
                     /locus_tag="IWO09_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0262793.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IWO09_000168"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IWO09_000168"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0262794.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IWO09_000169"
     CDS             1802..1978
                     /locus_tag="IWO09_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0262795.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IWO09_000170"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IWO09_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0262796.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IWO09_000171"
     CDS             2593..3060
                     /locus_tag="IWO09_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0262797.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IWO09_000172"
     CDS             3145..4029
                     /locus_tag="IWO09_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0262798.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IWO09_000173"
     CDS             4146..4379
                     /locus_tag="IWO09_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0262799.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IWO09_000174"
     tRNA            4467..4547
                     /locus_tag="IWO09_000174"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IWO09_000175"
     tRNA            4554..4625
                     /locus_tag="IWO09_000175"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IWO09_000176"
     CDS             5140..5847
                     /locus_tag="IWO09_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0262800.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IWO09_000177"
     CDS             complement(5907..6551)
                     /locus_tag="IWO09_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0262801.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IWO09_000178"
     CDS             6678..6965
                     /locus_tag="IWO09_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262802.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IWO09_000179"
     CDS             7073..7393
                     /locus_tag="IWO09_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0262803.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="IWO09_000180"
     CDS             7474..8787
                     /locus_tag="IWO09_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0262804.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="IWO09_000181"
     CDS             8784..9503
                     /locus_tag="IWO09_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0262805.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="IWO09_000182"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="IWO09_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0262806.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="IWO09_000183"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="IWO09_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0262807.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IWO09_000184"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IWO09_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0262808.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IWO09_000185"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IWO09_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0262809.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IWO09_000186"
     CDS             14940..15833
                     /locus_tag="IWO09_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0262810.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IWO09_000187"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IWO09_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0262811.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IWO09_000188"
     CDS             17770..18627
                     /locus_tag="IWO09_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0262812.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IWO09_000189"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IWO09_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0262813.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IWO09_000190"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IWO09_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0262814.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IWO09_000191"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IWO09_000191"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0262815.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IWO09_000192"
     CDS             21383..22141
                     /locus_tag="IWO09_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0262816.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IWO09_000193"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IWO09_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0262817.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="IWO09_000194"
     CDS             23069..24100
                     /locus_tag="IWO09_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0262818.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="IWO09_000195"
     CDS             24097..24864
                     /locus_tag="IWO09_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0262819.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="IWO09_000196"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="IWO09_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0262820.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26832..27149
                     /locus_tag="IWO09_000197"
     CDS             26832..27149
                     /locus_tag="IWO09_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0262821.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27149..27499
                     /locus_tag="IWO09_000198"
     CDS             27149..27499
                     /locus_tag="IWO09_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0262822.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="IWO09_000199"
     CDS             27666..28604
                     /locus_tag="IWO09_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0262823.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="IWO09_000200"
     CDS             28619..29449
                     /locus_tag="IWO09_000200"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0262824.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="IWO09_000201"
     CDS             29493..30518
                     /locus_tag="IWO09_000201"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0262825.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="IWO09_000202"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="IWO09_000202"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0262826.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="IWO09_000203"
     CDS             31596..32036
                     /locus_tag="IWO09_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0262827.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="IWO09_000204"
     CDS             32039..32641
                     /locus_tag="IWO09_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0262828.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32700..33806)
                     /locus_tag="IWO09_000205"
     CDS             complement(32700..33806)
                     /locus_tag="IWO09_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0262829.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34016..35017
                     /gene="ccpA"
                     /locus_tag="IWO09_000206"
     CDS             34016..35017
                     /gene="ccpA"
                     /locus_tag="IWO09_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0262830.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35354..37699)
                     /locus_tag="IWO09_000207"
     CDS             complement(35354..37699)
                     /locus_tag="IWO09_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0262831.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37978..38880
                     /locus_tag="IWO09_000208"
     CDS             37978..38880
                     /locus_tag="IWO09_000208"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0262832.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39093..40868
                     /locus_tag="IWO09_000209"
     CDS             39093..40868
                     /locus_tag="IWO09_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0262833.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40934..42520)
                     /locus_tag="IWO09_000210"
     CDS             complement(40934..42520)
                     /locus_tag="IWO09_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0262834.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42771..42995
                     /locus_tag="IWO09_000211"
     CDS             42771..42995
                     /locus_tag="IWO09_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0262835.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43137..44888
                     /locus_tag="IWO09_000212"
     CDS             43137..44888
                     /locus_tag="IWO09_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0262836.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44888..46672
                     /locus_tag="IWO09_000213"
     CDS             44888..46672
                     /locus_tag="IWO09_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0262837.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46998..47849
                     /locus_tag="IWO09_000214"
     CDS             46998..47849
                     /locus_tag="IWO09_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0262838.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47903..49654
                     /locus_tag="IWO09_000215"
     CDS             47903..49654
                     /locus_tag="IWO09_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0262839.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49658..49828
                     /locus_tag="IWO09_000216"
     CDS             49658..49828
                     /locus_tag="IWO09_000216"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262840.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49868..50770)
                     /locus_tag="IWO09_000217"
     CDS             complement(49868..50770)
                     /locus_tag="IWO09_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0262841.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51001..52404
                     /locus_tag="IWO09_000218"
     CDS             51001..52404
                     /locus_tag="IWO09_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0262842.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52404..53837
                     /gene="ascB"
                     /locus_tag="IWO09_000219"
     CDS             52404..53837
                     /gene="ascB"
                     /locus_tag="IWO09_000219"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0262843.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53982..54908
                     /gene="trxB"
                     /locus_tag="IWO09_000220"
     CDS             53982..54908
                     /gene="trxB"
                     /locus_tag="IWO09_000220"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0262844.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55122..56849
                     /locus_tag="IWO09_000221"
     CDS             55122..56849
                     /locus_tag="IWO09_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0262845.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56921..57214
                     /locus_tag="IWO09_000222"
     CDS             56921..57214
                     /locus_tag="IWO09_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0262846.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57306..58166)
                     /locus_tag="IWO09_000223"
     CDS             complement(57306..58166)
                     /locus_tag="IWO09_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0262847.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58258..58686
                     /locus_tag="IWO09_000224"
     CDS             58258..58686
                     /locus_tag="IWO09_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0262848.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58848..59024
                     /locus_tag="IWO09_000225"
     CDS             58848..59024
                     /locus_tag="IWO09_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0262849.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59051..59497
                     /locus_tag="IWO09_000226"
     CDS             59051..59497
                     /locus_tag="IWO09_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0262850.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="IWO09_000227"
     CDS             complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="IWO09_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0262851.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61736..62707
                     /locus_tag="IWO09_000228"
     CDS             61736..62707
                     /locus_tag="IWO09_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0262852.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62731..64923
                     /locus_tag="IWO09_000229"
     CDS             62731..64923
                     /locus_tag="IWO09_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0262853.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64940..65416
                     /gene="ybeY"
                     /locus_tag="IWO09_000230"
     CDS             64940..65416
                     /gene="ybeY"
                     /locus_tag="IWO09_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0262854.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65394..65795
                     /locus_tag="IWO09_000231"
     CDS             65394..65795
                     /locus_tag="IWO09_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0262855.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65810..66709
                     /locus_tag="IWO09_000232"
     CDS             65810..66709
                     /locus_tag="IWO09_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0262856.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66852..67664
                     /gene="recO"
                     /locus_tag="IWO09_000233"
     CDS             66852..67664
                     /gene="recO"
                     /locus_tag="IWO09_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0262857.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68048..68965
                     /gene="glyQ"
                     /locus_tag="IWO09_000234"
     CDS             68048..68965
                     /gene="glyQ"
                     /locus_tag="IWO09_000234"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0262858.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68967..71042
                     /locus_tag="IWO09_000235"
     CDS             68967..71042
                     /locus_tag="IWO09_000235"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0262859.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23266 a        12442 c        15206 g        20166 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtt
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgc gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga
    31021 tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc
    31201 cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag
    31681 tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa
    31741 tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta
    31801 atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga
    31921 aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc
    32101 cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc
    32161 ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga
    32221 gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc
    32281 aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga
    32341 tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc
    32401 agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga
    32461 tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga
    32581 tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta
    32641 aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct
    32701 tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt
    32761 gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt
    32821 tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg
    32881 aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag
    32941 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga
    33001 cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat tttttcttgg
    33061 aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa
    33121 acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta
    33181 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    33241 acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc agtcacgcct
    33301 tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc
    33361 agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat ggaagttaga
    33421 tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc
    33481 aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt
    33541 ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta
    33601 ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga
    33661 gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg
    33721 atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt
    33781 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    33841 aataattgta ccactgattt attaaaagac caacgttcta aaaactgaaa aaagtaaaaa
    33901 ttttcatgaa aacagagata aaacggttgc aattcttgag attatttgat attctatttt
    33961 agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga
    34021 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
    34081 ttcacgtgtc gtaaatggca atccaaacgt taaaccagca acaagaaaaa aagtcttgga
    34141 agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa
    34201 aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc
    34261 acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga
    34321 tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg
    34381 cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
    34441 aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat
    34501 tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa
    34561 aatcgctttt gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg
    34621 ctataaagaa gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc
    34681 acaatacaac ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac
    34741 agctgtctat gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg
    34801 tggcgtgaaa gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga
    34861 agtagctcgt cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc
    34921 tatgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
    34981 gccttatgga atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt
    35041 tgtgtgcctg ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg
    35101 tttgatgaat attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa
    35161 aaggggttat gacaaaattt tgttataatc ctttttttgt gtcttaaaaa agagagaaag
    35221 tggttgaaat tacatttatc gtatataaaa caatccgtgt atagatgctg cattttgttc
    35281 accaccatca aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt
    35341 gtattctatt aaatcaatca tctttctcat ctgattcttt agttgcttga ttcgctcttg
    35401 cataagctga agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa
    35461 atttaaaggc gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg
    35521 gtgtttggat ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag
    35581 atactttttt cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt
    35641 caggttttgc ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc
    35701 ttgctttgct gtccattcca gtattgtcat cgtgccctgc ccagctactg atggtaatag
    35761 acggagtaga gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    35821 cccaatctgc ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag
    35881 tgatttttgc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttccg
    35941 aatagatccg gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga
    36001 tggaatcgat gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt
    36061 tggtctgttg aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat
    36121 tgtcatttgc cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc
    36181 caccggaatt caacacatct tgatataagt gataagccgg gatattgttg gaccattcta
    36241 gtgactcacg gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct
    36301 cgccagcatt ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc
    36361 cgataagccc catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag
    36421 cttgacgttc tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa
    36481 cgaatccgta gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac
    36541 tgccagcacc atcatctaac atgtatccat aattttcaac gccattttgc aatgcagcat
    36601 agacatcttt atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg
    36661 ccttttgata ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag
    36721 ccaattgttg tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc
    36781 tgtcagtctg ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg
    36841 cttcttcgta ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat
    36901 cttttctggc aaggccggca gaaagatctt cttttagcgc gcctgtgttc gtgtatggac
    36961 tatacgtgat cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat
    37021 cttttgcatc tacaccaaag attccttttg cagcttcctg gactccagca atgttttgtc
    37081 ccttattatt acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg
    37141 aaaagtattt ttctacttgt gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg
    37201 tttcatctgt aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag
    37261 agccaatacc agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt
    37321 ggtgtttatc gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa
    37381 tcttatcaga tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat
    37441 tatctgcata aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt
    37501 ttttagtcgg caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa
    37561 tacctagagc tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa
    37621 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    37681 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    37741 caaataatag aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaaag
    37801 agttaaaacg tgagaaaatg atctttatta taaaagagtt cttaagcttt tctttagaga
    37861 aaatttcagt tactatggag aacagggcaa agaaaaatca atcattttct ttgtatatca
    37921 tcttaccaat atataggaat tgttagtaac aattgccttt caaggaggaa tccatccatg
    37981 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggagc agcagaagaa
    38041 gcagccagtt atggatcttc gacttttatg atctatacag gcgcccctca aaacactaga
    38101 agaaaagcca tcgaagagat gaatattgat gccggaaaag cgtacatggc agaaaaaggt
    38161 ctaacaaata tcgtcgttca tgccccttat atcattaatt tggggaatac gatcaaacca
    38221 gaaatgtttc cttttgccgt ccaatttctg agagaagaga ttgttcgtgc ggaagcttta
    38281 ggcgctactc aaattacact tcacccaggt gctcatgtag gggcaggaac agaagccgga
    38341 attgctagaa ttattgaagg cttgaatgaa gttttgacaa aagaacagac ggcacaaatc
    38401 gctttggaga caatggcagg taaaggtacg gaaatcggtc gaacatttga ggaattagcg
    38461 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga cacctgtcat
    38521 acgaatgatg ccggttataa tattaaagaa gacttttcag gtgtaatgga agagtttgat
    38581 aagatcatcg ggttagatcg gcttaaagta ctccatatca atgattctaa aaatccgcaa
    38641 ggttcgcata aagaccggca cgccaatatt ggttttggaa caatcggatt cgacacgctg
    38701 aataagatcg tccatgatcc aatgtttgac aatatttcga aaatcttgga aactccctat
    38761 gtaggcaaag ataaaaagaa agcttacgca ccatatggaa aagaaatcgc catgttcaaa
    38821 gcgcaaacat tcgatccaga agtgtttcct gagttattag cagaagaagt gatactctaa
    38881 gattttctat ctgacagcag caaaaaagga ccaggcacat tgccttgtcc ttttttgttt
    38941 aagaaacaaa ggagtacttt ttgcagtctt tttcatcatg catcttctcc cccctctttt
    39001 taaatttaga caaacaaggt atactggtag aaaagtaaaa aaacgaaaag tttcacaagc
    39061 aagtggcaaa acgtgattgg aaggacgaaa agatgctgaa cactttaaaa cccttgatca
    39121 accaactaaa aaagaaacat acatacttta ttcttggcat agccctatta tttacaatta
    39181 ttcgtttgta tttatatagt aaagcaatct atggaacaga catgtcggca acctatgatg
    39241 accagctgct gattcgctat gctcaaaata ttttggacgg taaatggcta ggggagtata
    39301 cgactacaac actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac
    39361 tgcatctttc ttatgcagtt ttgttcggca tgttcagtat ttttgccagt atattggttg
    39421 cactcgcgtt taaaccgatt gtgaagaaca agtatcttct tttaagtatc tatttttatt
    39481 ttttgttttc tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc
    39541 tcgtgattcc ggctgttgag atcattatcg cttgtttatt agctatttat taccgcaaat
    39601 acggttctag cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct
    39661 tttggttcat tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta
    39721 tcagtttcgt ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa
    39781 aaaaacgact ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc
    39841 ctatagttgg cttttccatc gtctatcaat ggatcgaata taaaaatgag acaacatacg
    39901 gtattgcaac catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga
    39961 tccgaataga tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg
    40021 ctgcactaaa taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg
    40081 acgaattata tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag
    40141 atatcatctt ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg
    40201 caaaagaaac caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca
    40261 agggaacaat caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga
    40321 tgagtgatct gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat
    40381 ataaagattt ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg
    40441 ctcaagaatt attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa
    40501 cacctaattt ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc
    40561 aagttctggc tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag
    40621 gaaccgtatt tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg
    40681 gattaatact gacacaattg gtttttcttt tcggtgtctc atggttttgt tcttatgcgc
    40741 cagaaaaaaa ggactatttt ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat
    40801 tcatcgtagt aacaagcatg ctagttttca gtaaattaaa tctgaaaaaa gctttatctt
    40861 ctaaataaaa agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg
    40921 gaaaaatcag tttttattcg atttcttcta ccgtttcctg caaaagctgt tcatattttt
    40981 tgtttccttt atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga
    41041 ttacataagc gatgtgatca gccaatgcta gttgagcgga agtggcttgc tcgccgaaga
    41101 aactttcgat ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga
    41161 accagccaag aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc
    41221 tgttggtcat catcagtaaa ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct
    41281 gggattcatt cattaagtta ttcaaagcgg catgttcttc gatcgttcct tttgtacttg
    41341 taaacaaaac acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta
    41401 gccaaatcgg gatacgcata tggatgaatc cttccatgat gacttgaccg gtcaacgtac
    41461 cagtaatagt agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggacaaga
    41521 taccagattt tgctacagca atcaaaatac cattgctcat agctgatgga tcagataaag
    41581 cttcatacag accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac
    41641 ccatgatcaa tagcagtgca ttgactagaa aagccattga caactggata ttagaatccc
    41701 aagtagaaaa acgtacagct tgtgctacat cttcttcgtt ttggtgatcg atcttccgag
    41761 tttgtgaaac tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc
    41821 ctaaagctcc agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac
    41881 tgttagggat aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat
    41941 agacaaaaac gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa
    42001 acccgacttt tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa
    42061 tcaccaatgg aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcaa
    42121 tatctgttgc cataatggct aattcagtca agatccataa aatgacacct aatgtcttac
    42181 ttgtgcgtgc tcggattgcc tgtgccaaat ccatttggct cacgattcct aattttgctg
    42241 ccatatactg taaaagcata gcaatcagac tagacatcaa aataactgac atcaataaat
    42301 attggaaatt ttgtcctccg gtgatcgaag tagaccagtt acccgggtcc atatacccaa
    42361 ctgctactaa cgcaccaggt ccagagtaag caagaagtgt tttaaaaaaa cttttcccct
    42421 ttggtacatc aactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa
    42481 gtcgatgctt atgaaattgt ttattggatt gattattcaa gaaaaaacca cctttcctat
    42541 ttatctacga ttacattcta cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg
    42601 cctaattttt tttcatggcg cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa
    42661 aaaaaccact attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt
    42721 agtaaaataa gtcattgaga gcaattcgga aatagaaagg agagatttat atgttatcaa
    42781 caggtattgt cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt
    42841 tcctagcgcg taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca
    42901 tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc tgagaagaaa attcgccaga
    42961 tgatgcagca aatgaagaac caagggaaaa aataggttct gacccaacga ctttttacgg
    43021 gctgattttt tcagtccgtt ttttatttcg taatttgtta gactattcag attattcagc
    43081 aaaaaagaca agcacagaca atcctaggct cttgataaag acaaaggagg agtaaaatgt
    43141 cgatttttag aaaattaggc tggttttttc gtcaggaaaa gaaacattat ttaattgggg
    43201 ttttctcgct attcgcagtg gccttggtac agttagttcc tccaaaagtg attgggatca
    43261 tcattgatga gatcgccgat aaaaatttat caataaagat cattttcggg tggatcttcc
    43321 tattgattat cgctgctttt ttacaatatc tttttcgtta tatctggcgg acgaatattt
    43381 ggggaagtgc agctcgattg gagaaagaac ttcggcagca gttattcaaa cattttacag
    43441 aaatggacaa tctcttttac caaaaatatc ggactgggga tttgatggct cacgcaacga
    43501 atgatttgaa tgcgatccag aatgtagcag gagctggtat cttgaccttt gcagattcag
    43561 tgattacggg cggaacaacg atcattgcga tgattttgtt tattgattgg cgcttaacac
    43621 tgattgcgct gattcctctg cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac
    43681 tccatgatgc ttttcgtgat tcgcaagaag ctttttctag tatcaatgat aagacacaag
    43741 aaagtatcac tgggatcaaa gtcattaaaa catttggaca agaaaaagaa gatctagcgg
    43801 attttgaaga aaagattgaa gatgcaatta ttaaaaacaa acgagtcaat tttttagact
    43861 ccttatttga tccattcatt accttgatta tcggtctgtc ttatgttgca acaattattt
    43921 taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg gcagatggtc tcatttatta
    43981 gttatatcgg tatgcttgtt tggccgatgt ttgcgattgg tcgattgttc aacgtattgg
    44041 aaagaggaaa tgcttcttat gatcgcgtca atgaactttt gcatgaaaag acgcatatcg
    44101 tggaacaact gggtgcagtg cagaccccgg caaaaggcga gctgaatgta gctatttcct
    44161 cttttaatta tccgaaagaa gaatcaccag cattagaaca aatcaagttt gcgatccaag
    44221 aaggagaaac attaggtatt gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat
    44281 tactgatgcg tgaatacgat gattatcaag ggaaaatcca atttggcgga cgttcgatca
    44341 aagaatattc attgaatgct ttattaggat caatcggcta tgtgcctcag gatcacttct
    44401 tgttttcgat gaccattcga gataatatcc gtttttctaa tccaaaactt tcagaagaag
    44461 aagtagagca tgcggcttct ttagcattta tcaacaatga aatcaaggaa ttaccaaaag
    44521 gttatgatac cttagttgga gaacgtggag tctctttatc tggcggacag aaacaaagga
    44581 tttcgattgc tcgagcattg atcaaaaatc ctgaattatt aatattagat gatgcactct
    44641 ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa tttaaaagaa ataagaaaag
    44701 agaaaacaac tatcattgca gctcaccgtt taagtagcgt gatgcatgca aaagaaatca
    44761 ttgttgtcga tcaagggaaa atcattgaac gaggaaccca tcatgagctg cttgcactag
    44821 gcggctggta tgaacaaatg tgggagaaac agcaattaga agcgaagata gaaggaggcg
    44881 aggcctgatg gatgaaaatt atcaatcgga atggacaaaa tcgattccac ttaaagaaca
    44941 gaaaaagatc atcaaacgac tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt
    45001 tgctatctta tttgcgtttg ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca
    45061 aacttttatg gatgatcatc tggctaaaca gtcggctacg acacaagtta ttttgttttt
    45121 tgctggtatg tacttatttg gagtcatagt aaaaagtatc atttggtttt tccaatggta
    45181 tttgtattcc atggcctcat tgaagaccta tcagtatatt agggtaaaat tatttgaaaa
    45241 gctgcataca ctaggtatgc gttattttga tcagacaccg gcaggatcaa ccgtttctcg
    45301 agtgacgaat gacacagaaa cgctatttga attttggtac gtctttttga tggtattgac
    45361 tgggatcttt gcagtcgttt cttcattcct tgccatgttt caaatcaatg gagaaattgc
    45421 cttgtggact ttggtgtttt tgccgatttt aggaatcgtc atctggtatt accagaaatt
    45481 cagttctcgt atttaccgaa atatgcggga aaaattaagt cagctgaaca cgaaattgaa
    45541 tgaatcgatt tccggcatgc gtattatcca gcagtttcgc caagaagctc gtttagctaa
    45601 agaatttgaa caagtgaaca atgagtattt agacacgcgt tatgcgatga tcaaaacgaa
    45661 ttcgattctg ttaagtccga tcatcaattt tctatatgca ttggcgattg cgttggtgtt
    45721 gactttgttt gggatcgatg cgttgcattc tcccgtagaa gttggactga tctatgcatt
    45781 tacgacctat gtccagggat tttttaatcc aatgtctcaa atgatggact ttttgagcat
    45841 cttcacagat gggatggttg ctggtagtcg tatcttgaag attatggata cggaagaata
    45901 tgcaccacaa caaaaccctg gagcagatca aatgattact tcaggtaaga ttgagtttcg
    45961 caatgtaagc ttttcttatg atggagagca caatgtatta aacaatatct cttttgttgc
    46021 taatccggga gagaccgttg cattagtggg acacaccgga agcggaaaaa gctcgatcat
    46081 caacgtattg atgcggtttt acgaatttta tgaaggtcag atattgattg atgatcatga
    46141 cattcgagaa tatccaatgg aagagttacg taagaaaatg gggctggtct tgcaagatgc
    46201 tttcttgttt tatggggata tcgcaggaaa tattcggatg atgaatccag ctatcacaga
    46261 cgaacagata cgccatgcgg ctgagtttgt tcaagcaaac aaattcatcg atgaattacc
    46321 aaaaggatat cactcaaaag tgatcgaaca tggtgccagc tattctagtg gacaacgaca
    46381 gctgatcact tttgcgcgaa caatcgtgac tgatcctaaa attctagtat tggatgaagc
    46441 aacagcaaat atcgatactg aaacagaagg attgatccaa gaaggattgg caaaaatgcg
    46501 tcaaggacgg acaacgattg cgattgccca tcggctgtca acgattcgag atgcagaatt
    46561 gattttagtt ttagacaaag gtcatatagt agaacgcggg aatcatgaaa cacttctagc
    46621 gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc agtggtatat aaaagatgtt
    46681 gtgacagtag aattttgacc taagcactag agtgaaaata aaacatagct tctcatattt
    46741 ttcatctgtg agagttagtg tcgtagtcac cagatttaaa aaaagattcg acagctagtc
    46801 agaagtgcct aatagccact aagaaagcgt tgacaaaagc atttgctttc gataatataa
    46861 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    46921 atggtttcat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatacatc
    46981 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    47041 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    47101 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    47161 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    47221 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    47281 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    47341 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    47401 aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat
    47461 ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccagcaagac aatacctacc
    47521 tgtttactat aacaatacag gatattctaa atatcgtccg ctattactat aagattacat
    47581 ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cttacgtttt tttctgacac
    47641 gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg tttcaggtta
    47701 ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg
    47761 agacgttcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc catactgcgc
    47821 gtttagtgca tgagaatcgt tgtaaataaa aaaaggagga taaattaaca agaacgtatg
    47881 aaataagggt agtgacattc agttggctaa accttccact aataaattgg agggaataga
    47941 aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa
    48001 tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa
    48061 agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc gagcgggagg
    48121 gcagtatcaa gttgtcattg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc
    48181 aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct
    48241 tattgatgga atatcgggct gtttccagcc attccttgct atcctagctg ctggcgggat
    48301 gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg gttctggcac
    48361 atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg
    48421 tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc
    48481 attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactaac
    48541 gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc
    48601 ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc
    48661 tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt
    48721 tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg gaccagtatt
    48781 cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt
    48841 tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg gtctccattg
    48901 ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atattatgac
    48961 acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct actttaagct
    49021 tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta ttgtggggat
    49081 tacagagcca gctatttatg ggtttacatt accacgtaaa aaaatattta tttttacctg
    49141 tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt cttggaatca
    49201 aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacaga
    49261 tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatccttta cgctgacttt
    49321 tttcttttgg aaggatgaag caggtgaaac agatgatata cagaaaaaat caggtaaaga
    49381 aattgtaaaa acaccgatac aaggacaaat cgctccacta aatgcagcaa aggatgcagc
    49441 ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg
    49501 tgcccctttt gacggaacag tcatgaccct gttcccgacg aaacaggcaa ttggaatggt
    49561 ttctgaaaca gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg
    49621 gaaatatttt gaaagcttag tgcagcaagg ataaagagtg aaacaaggcg atctgttgct
    49681 acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt
    49741 caccaattac acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga
    49801 cgatgcactt atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact
    49861 attcttttta ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc
    49921 tgatagggcg tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat
    49981 aattttctgc aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga
    50041 tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg
    50101 tactcattgc agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatgat
    50161 tttgtttcaa tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca
    50221 gaatcccgca catatcactc gtgcaattgt acgcttcata gaatgattca ccaaaagtgg
    50281 tgacaatcgt gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca
    50341 aagtattcga gttactggcg aaaatcacga ttttttttgc ttgaagcatt agttgtttgg
    50401 ctttttgcag ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta
    50461 aagaagccat tttatttgcg atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg
    50521 catctacgtc ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa
    50581 aactgaccca gccatcaaaa cccatttttt ttgctacacg gatgagtgta gaggggtgga
    50641 cataacatcg ctcagcaatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta
    50701 agagaaactg gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac
    50761 gtaataacat gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa
    50821 tgatttgtaa acggatttat actatataac ttaaaataga cattttttga caagatattt
    50881 acaaaaatcc actagaaaac agtaaatttt gtaaatgtta ctggaaagaa acagatttct
    50941 attgtttgtt ttgtttacaa aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac
    51001 atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg
    51061 atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat
    51121 gtagaagaag gaaaagaaaa gcttgagatc gttagtcaga tgtctcagcg tatgtgggat
    51181 gcttctccgt atgaagtcaa gatctatgca actctggatc gggaagaagc attgaaagat
    51241 gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag
    51301 cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc
    51361 aaagcttttc gtaccgttcc agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt
    51421 ccaaatgctt ggctgatcaa tttcgcgaac ccaagtggga tggtgacaga agcagttatc
    51481 agatatggca aatgggataa agtgattgga ttatgcaatg tgccagtgat ggcgatgatg
    51541 acagaaccag caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta
    51601 aatcatttcc attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg
    51661 attgatcgtt tatacgatga agagaatgga ttaccgaaaa atatctttga tatcccgttt
    51721 ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat
    51781 cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg
    51841 cagcaagtga aagaaacaga agcagagcta tttgaattat acaaagatcc agaattagac
    51901 cataaacctg aacaattgca aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa
    51961 acgattgctt caatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat
    52021 ggagctgttc ctgacctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca
    52081 caaggagcga gaaatgtagc gtttggttct cttcctgctg ctgaaagagg ctggttgcaa
    52141 gtaatgaaga atatggaatt gctgacgatc gaagcagctg tgactggtga ttatgggaca
    52201 gctttacagg cgtttacgat caatccactt atacgatcag gaaagacagc aaaacggatc
    52261 atggatgagt tgtttattgc acaccagcag tatttaccgc aattcaagga gacaatcagc
    52321 cgattaaaag aagaaggaat cgaagttcaa gatgaactgg caagagaatt atcaggtaat
    52381 acacatataa tggaggaagt ataatgggat tcagaaaaga ttttttatgg ggcggtgcta
    52441 cagcagccaa tcaatgtgag ggcggttatg atgaaggtgg acgcggacta gcaaacgtag
    52501 atgttgtgcc aatcggaaaa gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg
    52561 aatttgatga ggatcatttt tatccagcaa aaattgcaat cgatatgtat catcgttata
    52621 aagaagatat tgctttgttt gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct
    52681 ggagtcgtat ttttccaatg ggggatgaga aagagccgaa tgaagctggc ttgaaatttt
    52741 atgaggatgc ctttaaagaa tgccaaaagc acggaattga gccacttgtg acgattacgc
    52801 attttgattg tccaatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg
    52861 ttggttttta tgaaaatctg tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat
    52921 attggctgac attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc
    52981 tgtattttga aaaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt
    53041 tggttgcaag tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag
    53101 gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg
    53161 cttctagaaa atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat
    53221 atcccgctta tgcattgaaa gaaatggagc gaaatggcat tcagatcgaa atggaaccag
    53281 gggatgccgc attgctgaaa gaacatactg ttgattttat ctctttttct tattatgctt
    53341 cccgagtttc gactaccgac aacgaactct tggaacagac tgcaggaaat atctttgctt
    53401 ctgtcaaaaa tccatatctg caagcaagtg aatgggggtg gcaaattgat ccattaggct
    53461 tgagaataac gatgaatgat atttatgatc gttatcaaaa accattgttt attgtagaaa
    53521 acggcttagg tgcagtcgat acgccagatg aaaatggtta tgtggaagat gattaccgaa
    53581 tcgattacct ggcagcgcat atccaggcga tgaaggatgc agtagaacta gacggagtcg
    53641 atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt accggtgaga
    53701 tgaagaaaag gtacggattc atttatgtcg atcgtgataa ccaaggaaat ggcacattag
    53761 cccgttcaaa gaaaaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa
    53821 atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg
    53881 agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct
    53941 gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta
    54001 ttggcgcagg tcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg
    54061 tattgatgat cgaacaaggt gcccctggcg gccaaatgaa caatacggca gaaatcgaaa
    54121 attatcctgg attcgattcg atcatgggcc ccgatctagc ttataagatg tatgaaagtg
    54181 tctcaagatt tgggacagaa aatgcttatg gaatcgttca agatatcaag gatcacggca
    54241 gccataaaga agtcatctgt gaagataaaa gttacgaagg aaaaacagtc attatcgcaa
    54301 caggatgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgaggcg
    54361 tttcttactg tgcagtctgt gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg
    54421 ggggtgggga ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag
    54481 tgatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg
    54541 caaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa
    54601 tggtagtgac aggtgtgaaa gcacgtaatg tcaaaaccgg caatgaattt gatttagcag
    54661 cagacggagt attcatctat gttggactgg atccattaac tgcacctttt agagaagcag
    54721 gtttgacgaa cgaagctggc tggattccaa cagacaacga aatgaaaacg aatatgccag
    54781 gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact acagctgttg
    54841 gtgacggcgg tattgctgga caacaagtgt ataaatactt ggagtcttta gaagaaacaa
    54901 cagcataagt ttaaaaagag tagaagaacg ggttcaattg ttcttttact cttttttttc
    54961 gcgtgaaatt tactgaatct gatttatgcg aacacgtcca acaccttttg ataaaagaat
    55021 cctttcttga tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta
    55081 tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt
    55141 atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc
    55201 tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg
    55261 caggtatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac
    55321 aagcaacaga aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag
    55381 gtgtcgctat cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca
    55441 aaacactagc acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag
    55501 agctttcttt tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc
    55561 acaacccagc ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc
    55621 ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg
    55681 aagtattatc tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag
    55741 tggacgctgc ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa
    55801 caatggggaa agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg
    55861 gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg
    55921 tggcagatcc agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg
    55981 aatatgctat acgacttgga gaaaaagaag gagctgactt gctgattgca acagatcctg
    56041 atgcagaccg tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg
    56101 ggaaccaaat cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca
    56161 ctttaccaga aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa
    56221 ttgcggaaca ctacggtgct accatgttca atgtgttgac agggtttaaa ttcattgctg
    56281 aaaaaatcca acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa
    56341 gttatggtta tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt
    56401 tattagcaga agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc
    56461 aaacgatttt tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg
    56521 gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga
    56581 cagaatttgc cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag
    56641 accgtgaagg aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat
    56701 tggaagaaaa tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt
    56761 atattggtgt aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag
    56821 cgattatcaa aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa
    56881 ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc
    56941 aaaagtagag aaagtcagtc aattattcaa aatgttgagt gatcccactc gtcttaaaat
    57001 tctgctatat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat
    57061 ggagcagtct gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc
    57121 tcatcgcgag ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt
    57181 gaaccagaca ctcaaacatg tggaacatca ataacgatag gtgaaaatag ataaacactg
    57241 gagtgtgaca acgaagaaat ttcgttgtca cactccagtg ttttttaaga aaaagacaaa
    57301 aatgattaat cgcttttttc tgttgtttca gttccataat aactatgatc ttctaggtct
    57361 aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac attgatcact
    57421 acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg
    57481 atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata
    57541 ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac
    57601 agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta
    57661 tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca
    57721 tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt
    57781 gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt
    57841 ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta
    57901 aataattgat gttcttcaca aaaagaacgt gttatctcga tcagttcttg attgattaat
    57961 gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt
    58021 tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata
    58081 gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta
    58141 acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta
    58201 ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg
    58261 aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat
    58321 actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct
    58381 aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttac
    58441 cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt aaatggtgaa
    58501 atgaagtttc gttttcattg ctgtcagggg gaaatcggcc atcaccatca ttttatctgt
    58561 acagtgtgcg gaacaacgaa agaaatccat atgtgtccaa tgaattattt cgaagaacag
    58621 ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt
    58681 caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga
    58741 cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg agatatattt
    58801 caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag
    58861 tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa
    58921 aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac
    58981 gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg
    59041 agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc
    59101 gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca
    59161 gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat
    59221 gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga
    59281 aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga
    59341 agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga
    59401 atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca
    59461 agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca
    59521 gtgattgtcc gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag
    59581 aataagattc tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg
    59641 tttgatttgg ttattcttct ggtagttcat ttgcttcttg tttataccct gataggcgat
    59701 aggcgtaata aaagagtaag ctgccagcgt aaccaagtaa cgttgggacc atccagccaa
    59761 gtcctaatgc agcaaaagga acgtgatgat gataaaatcc tagtaagctt gctacgacag
    59821 tttgctgact cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag
    59881 aaaagaacat cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg
    59941 aaagcaagat taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt
    60001 ggatgatatt atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca
    60061 gccatgtcat ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa
    60121 cgagtcccat agcggtagtg aagacaccta atgtgaccat tacccctaag aagctagcac
    60181 cgaagttacc taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata
    60241 aaggcatctt attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt
    60301 ctaacgcaac gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa
    60361 aaccaagatc acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat
    60421 caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg
    60481 cagcagattg gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta
    60541 ataatgcaag aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat
    60601 gtacagtaag gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg
    60661 aatcatattt ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc
    60721 gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat
    60781 accattttcc gacaggttta gctaagtcat acagaccatc actttttgtc actactacag
    60841 ctaagatagc taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga
    60901 tccagtttga tccagccatt tgacctaagt ggacaggaaa gatcagatta cctgacccga
    60961 agaacatccc gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg
    61021 tttcttccat aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca
    61081 gaaactagaa atgccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa
    61141 aaagcccttt tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg
    61201 cttaaaaaag cttcttcatt cacggacgct tttcgataaa gttatccgct agcctgataa
    61261 tggaggcagc cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt
    61321 cgatttattg tcatttctca cttacatgac tcactgggaa taaatcgctg aatagcctac
    61381 tgttttcctc gacgctttgc tgtatattta tttgttgacg ttataataca cctgtttttt
    61441 ttgaaatgca agaatatttc aaatattttg ttttcttttt ttgaaacaag aaaaataccc
    61501 agagaatact tgctaagtta gacggaatac ttgtcctcta tcttatgcat agcttacatg
    61561 atcagctttt tttattcaaa cgagaccttt agagaatgga agcagctatt ttttaacgtt
    61621 tcttggtact tatggttaag caactctttt agaacctttt cttatttgct atatagtttc
    61681 ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac
    61741 ggaagagtct agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt
    61801 aggtgcacac gacaagcata taaaactgat tgaagaaaca acagaaacaa ccatccatac
    61861 acgtggagaa atcattcaga tcattggcga acaatcccag atttccttgg ctgcctcagt
    61921 cattcggaca ttgcaggaat tgatcaaacg aggaatccat gtaagtacac cagatgtcgt
    61981 gacggcactg aaaatggggc aaaaaggcac attagattat tttgttgaaa tgtatgagga
    62041 agagatcgtc aaagaccgta atggaaaacc aatacgggta aaaaacagcg gccaaaagaa
    62101 atatgtcgaa tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg
    62161 aaaaactttt cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa
    62221 aatcattttg acgcggcctg ctgtggaagc aggggaaaat ctaggctttc ttcccgggga
    62281 tctaaaagaa aaagtagatc cttatctgcg tcctgtttat gatgctttat accaaatttt
    62341 tggattagat catacgaacc gattgatgga acgaggcgtg atcgaaattg ctcctttagc
    62401 ttacatgcgg gggcggacat tggatgatgc attcgttatt ttggatgaag cacagaacac
    62461 gacagttgct cagatgaaaa tgttcctgac acgtcttggt tatcaatcaa aaatgattgt
    62521 caatggagat acgagtcaga ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc
    62581 agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt
    62641 tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca
    62701 agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa
    62761 gaaaaacttg gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc
    62821 ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca
    62881 gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga
    62941 aaactggcag cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac
    63001 cagaacaatc ggatcaagca actatttgaa ttgatcgata aaacgaatga tgccatcaac
    63061 aaatcttatg acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact
    63121 acagaagagc ggatcgccgc gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt
    63181 gcgttttatc aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact
    63241 gagatccata ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa
    63301 gttcgtgaaa gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg
    63361 aatgtcacac cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg
    63421 aatgacgtat tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta
    63481 caaccagtga tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct
    63541 tcagctatga aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg
    63601 ctaattgcca tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt
    63661 caattccaaa atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca
    63721 gtcagcatag tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc
    63781 ccgctgtttt atcccgctgc ttttgcacca cttgttttaa gctttttctt gaaccgaaga
    63841 gcagggatca tggctggtat tttccaagca gtatctgcac ttttcatttt ttatgattcg
    63901 attggcacga actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca
    63961 acgattgtca gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt
    64021 atttttcctg tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat
    64081 gcaacgactt ggttaatgct tgtctgcggg ttcactggtg cactcttctc attcttgctg
    64141 acgatcggtc tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta
    64201 aatgaactca gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt
    64261 acttatcatc atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc
    64321 ggacgttctt tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat
    64381 gctagctttt tcgttgagaa cttgccttca ggagcggaaa acccgcacaa tttcttattg
    64441 ccagaagaca gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa
    64501 gattataata tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta
    64561 atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc
    64621 cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt
    64681 tgtgaggctg cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc
    64741 cataatctta tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg
    64801 aaagaaatcc gaaaagtgga aaaatcattg atcagcggtt taagtagcac cttccattct
    64861 cggatcaaat atcctaaaat gaaaacagaa gttgagaaga taaaggaaga acaagaagaa
    64921 tagaaagaag cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga
    64981 agagaaaatg aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc
    65041 agaggaaaca gaaatgtctg ttactttcat ggacaatgcg gcaatccaag taatcaatcg
    65101 tgattaccgt ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg
    65161 cgaagacgaa atcccagtga tttttgaaga tggagagaat ccattgcctc gtgtactagg
    65221 agacatcatg atttctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata
    65281 tgatcgggaa cttggctttt tagcggtaca tggattcttg catatcaacg ggtatgacca
    65341 tatgacacca gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta
    65401 tggactcaaa agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg
    65461 ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt
    65521 tcggtatcct tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat
    65581 ggctgctttt agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa
    65641 atgtagtgga catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata
    65701 tggcagctgg agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat
    65761 ttgtccctaa aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca
    65821 taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa
    65881 ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa
    65941 aatccaagga gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat
    66001 ccataaacca aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa
    66061 agaagtggat gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc ggggagacga
    66121 ttttattatt gaacgtctaa aaaacgtgca ttctccagtt tatctgatta tcaataaaat
    66181 cgataagatc catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc
    66241 ttttgcacaa gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga
    66301 tgtattagtc agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga
    66361 tcatcctgaa tactttatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg
    66421 agacgaagtg cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga
    66481 taaaatccat atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat
    66541 cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa
    66601 tcttttagga gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga
    66661 caaaaaagtc tatctgcaag actttggcta ccgaaaagac gaatactgat attctgaact
    66721 tcttataggt taagtataga aataaactgg ataaaaatcc gcacgttgag aaaaacggcg
    66781 gaagatcgaa acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta
    66841 ggagggagaa tatgagccag cttgcagaat cgaaaggctt gattttatct agccgtaatt
    66901 acaaagaaaa agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt
    66961 atatcaaagg aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg
    67021 aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag
    67081 acgtccatgc gtttcggaac atccaagaag atatcttttt gagcggttat ggtacctatt
    67141 tgctaaatct agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat
    67201 ttacgcaaca agctctgcag cggatggatc aaggagatga tgcagaaatc atcacgaata
    67261 tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg
    67321 tatgcgggga aacaaaagga aaatttgact attcttccaa atctggcggt gtgatatgcg
    67381 agaaacactg gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg
    67441 ttcgaatgtt ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa
    67501 cgaagcaggc aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc
    67561 taaaaagcaa aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag
    67621 agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa
    67681 aaaaatcaaa actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa
    67741 tagatcgttc actgagagga tctattatat aaacaagaaa actgcaaaat gaatacgtac
    67801 attgaccaag atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac
    67861 ccaaacgatt tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt
    67921 cggaagtagg gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac
    67981 tttttgtcgt tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc
    68041 aaaacagatg agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg
    68101 gtcatcaaac ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag caggtacgat
    68161 gagcccatat acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga
    68221 gccatcccgc cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca
    68281 tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga
    68341 aagcttgcgt cttttaggaa ttgatccatt agaacacgat attcgttttg ttgaagacaa
    68401 ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat
    68461 ggagatcaca caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac
    68521 ttctgagatc acttatggac tagaacgtct tgcttcatat attcaagaag ttgaaagtgt
    68581 ctatgatctg gaatgggctg atggtgtgaa atatggcgag atcttcaaac aaccagaata
    68641 tgaacattcc aaatattctt ttgaagtaag tgaccaagac ttgctactaa gcaactttga
    68701 ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga
    68761 ttatattttg aaatgcagcc atacattcaa tttattagat gctcgcggtg cagtatccgt
    68821 aacagaacga gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat
    68881 ctttgtggca gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc
    68941 agaaaacatg aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg
    69001 aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa
    69061 aatttttaga tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc
    69121 gcttagcaat caaagtaaca aacattccag aacgtcaagc agacagtgaa gaagaagtca
    69181 aaggaccagc caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag
    69241 gatttgtccg cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg
    69301 tggaatatgt ctatgtaacg aaatacactc acggcaaaga agcgaaagaa gtattgacag
    69361 gtttagcaga tgtcattaaa agcctcactt tcccagtaac gatgcattgg gcagaatacg
    69421 attttgaata tatccgccct attcactgga tcgtggcatt gttagatgac gaagtaatcc
    69481 cgtttgagat cttagatgtc aaaacaggca gagcctctcg aggacatcgt ttcttaggcg
    69541 aagatgtgac atttgcgcat gcaaaagaat atgaagaaaa attgaatgaa caattcgtta
    69601 ttgcttcacc gcaaaaaaga caagaaatga tcgttacaca agcaaatgaa ttagccgaaa
    69661 aaaatcactg ggttattgat ctagacgaag atttactaga agaagtcaac aatttagtcg
    69721 aatatccaac tgtcttttct ggcgacttcg aagaaaaata tctttctgtt cctgaagaag
    69781 tactagtcac ttctatgaaa gaacatcaac gttatttcga ggtacggaac gatcaaggta
    69841 tgctgcttcc tcattttatt tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg
    69901 ttcgaggcaa tcaaaaagta ttgacggctc gtttagaaga tgcggaattc ttttataatg
    69961 aagacaagaa agcttcaatc gaccaatacg ttgaaaaact aaaaactgtt actttccatg
    70021 aaaaaatcgg tagtatctat gagaaaatgc agcgggtaca agtcattgcc cgtatcattg
    70081 gcgaacaagt aggactatct gaggaagaat tgatggatct gcaacgagct gctgaaatct
    70141 ataaatttga tctagtaacc aacatggtag gggaatttcc tgaactacaa gggatcatgg
    70201 gcgaaaaata tgctctgctg aaaggcgaga aacctgctgt agcaacagcg attcgtgagc
    70261 attacatgcc tatatccagt gaaggagagc tacctcagtc tacagtaggt tctgttttag
    70321 cgattgcaga taaattagat agcatttttt ctttctttgc agttgggatg attccaagcg
    70381 ggtcaaacga tccttatgcc ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat
    70441 cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac gattagtgat gttatcagta
    70501 aagatacgga tcgtttcggc attggtttat cagaagggca acaacaggtg atcgacttta
    70561 tcaaaggccg tctgcgccaa ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag
    70621 ctgttttgaa tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca
    70681 agcagcatct tgcagatgaa gacttcaaac catcgatgga agctttgaca cgtgtcgtga
    70741 acttagcgaa aaaagcggag cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa
    70801 atgaagcgga gaaagaactg cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc
    70861 aaacgatcaa cgaaaattat gatcagcttg taagccttcg cccactgatc gaaaattatt
    70921 ttgatcagac gatggtcatg gcagaagaac cagcagtacg taagaaccga ttaacgcaat
    70981 taagtcaaat tgctgccatg gctttgtcgc tagcaagttt agatcaaatc atcacaaaat
    71041 aatttctaat atgtaaataa accagagaat aggcacaaaa
//
LOCUS       DACECJ010000004        68718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.004, whole genome shotgun sequence.
ACCESSION   DACECJ010000004 DACECJ010000000
VERSION     DACECJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.004"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            399..1310
                     /locus_tag="IWO09_000236"
     CDS             399..1310
                     /locus_tag="IWO09_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0262860.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1527..1964
                     /locus_tag="IWO09_000237"
     CDS             1527..1964
                     /locus_tag="IWO09_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262861.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2059..2943
                     /locus_tag="IWO09_000238"
     CDS             2059..2943
                     /locus_tag="IWO09_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0262862.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3167..6334
                     /locus_tag="IWO09_000239"
     CDS             3167..6334
                     /locus_tag="IWO09_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262863.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6354..6899
                     /locus_tag="IWO09_000240"
     CDS             6354..6899
                     /locus_tag="IWO09_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0262864.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6927..7382
                     /locus_tag="IWO09_000241"
     CDS             6927..7382
                     /locus_tag="IWO09_000241"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0262865.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7379..8137
                     /locus_tag="IWO09_000242"
     CDS             7379..8137
                     /locus_tag="IWO09_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0262866.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8223..8963)
                     /locus_tag="IWO09_000243"
     CDS             complement(8223..8963)
                     /locus_tag="IWO09_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0262867.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            9024..10277
                     /locus_tag="IWO09_000244"
     CDS             9024..10277
                     /locus_tag="IWO09_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0262868.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10599..10826
                     /locus_tag="IWO09_000245"
     CDS             10599..10826
                     /locus_tag="IWO09_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0262869.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10949..11275
                     /locus_tag="IWO09_000246"
     CDS             10949..11275
                     /locus_tag="IWO09_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0262870.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11343..12056
                     /locus_tag="IWO09_000247"
     CDS             11343..12056
                     /locus_tag="IWO09_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0262871.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12366..13217
                     /locus_tag="IWO09_000248"
     CDS             12366..13217
                     /locus_tag="IWO09_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0262872.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13338..15203
                     /locus_tag="IWO09_000249"
     CDS             13338..15203
                     /locus_tag="IWO09_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0262873.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15251..16660
                     /locus_tag="IWO09_000250"
     CDS             15251..16660
                     /locus_tag="IWO09_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0262874.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            16768..17469
                     /locus_tag="IWO09_000251"
     CDS             16768..17469
                     /locus_tag="IWO09_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0262875.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            17628..18107
                     /locus_tag="IWO09_000252"
                     /pseudo
     CDS             17628..18107
                     /locus_tag="IWO09_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            18955..19530
                     /locus_tag="IWO09_000253"
     CDS             18955..19530
                     /locus_tag="IWO09_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0262876.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            19530..19970
                     /locus_tag="IWO09_000254"
     CDS             19530..19970
                     /locus_tag="IWO09_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0262877.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            19986..20687
                     /locus_tag="IWO09_000255"
     CDS             19986..20687
                     /locus_tag="IWO09_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0262878.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21175..21600)
                     /locus_tag="IWO09_000256"
     CDS             complement(21175..21600)
                     /locus_tag="IWO09_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262879.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            21974..22945
                     /locus_tag="IWO09_000257"
     CDS             21974..22945
                     /locus_tag="IWO09_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0262880.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            22977..24374
                     /locus_tag="IWO09_000258"
     CDS             22977..24374
                     /locus_tag="IWO09_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0262881.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            24533..25156
                     /locus_tag="IWO09_000259"
     CDS             24533..25156
                     /locus_tag="IWO09_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262882.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(25382..25906)
                     /locus_tag="IWO09_000260"
     CDS             complement(25382..25906)
                     /locus_tag="IWO09_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0262883.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(25899..26372)
                     /gene="tsaE"
                     /locus_tag="IWO09_000261"
     CDS             complement(25899..26372)
                     /gene="tsaE"
                     /locus_tag="IWO09_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0262884.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(26495..27475)
                     /gene="pta"
                     /locus_tag="IWO09_000262"
     CDS             complement(26495..27475)
                     /gene="pta"
                     /locus_tag="IWO09_000262"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0262885.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(27628..28308)
                     /locus_tag="IWO09_000263"
     CDS             complement(27628..28308)
                     /locus_tag="IWO09_000263"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0262886.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            28434..29303
                     /locus_tag="IWO09_000264"
     CDS             28434..29303
                     /locus_tag="IWO09_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0262887.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            29329..29844
                     /locus_tag="IWO09_000265"
     CDS             29329..29844
                     /locus_tag="IWO09_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262888.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(29891..30454)
                     /locus_tag="IWO09_000266"
     CDS             complement(29891..30454)
                     /locus_tag="IWO09_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0262889.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(30509..30688)
                     /locus_tag="IWO09_000267"
     CDS             complement(30509..30688)
                     /locus_tag="IWO09_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262890.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(30765..31274)
                     /locus_tag="IWO09_000268"
     CDS             complement(30765..31274)
                     /locus_tag="IWO09_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262891.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(31370..31750)
                     /locus_tag="IWO09_000269"
     CDS             complement(31370..31750)
                     /locus_tag="IWO09_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0262892.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            32080..32829
                     /locus_tag="IWO09_000270"
     CDS             32080..32829
                     /locus_tag="IWO09_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0262893.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(33013..34290)
                     /locus_tag="IWO09_000271"
     CDS             complement(33013..34290)
                     /locus_tag="IWO09_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262894.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(34369..35013)
                     /gene="pcp"
                     /locus_tag="IWO09_000272"
     CDS             complement(34369..35013)
                     /gene="pcp"
                     /locus_tag="IWO09_000272"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0262895.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(35212..36156)
                     /locus_tag="IWO09_000273"
     CDS             complement(35212..36156)
                     /locus_tag="IWO09_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0262896.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(36158..36844)
                     /locus_tag="IWO09_000274"
     CDS             complement(36158..36844)
                     /locus_tag="IWO09_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0262897.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            37318..37638
                     /locus_tag="IWO09_000275"
     CDS             37318..37638
                     /locus_tag="IWO09_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0262898.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            37792..38697
                     /locus_tag="IWO09_000276"
     CDS             37792..38697
                     /locus_tag="IWO09_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0262899.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            38694..39467
                     /locus_tag="IWO09_000277"
     CDS             38694..39467
                     /locus_tag="IWO09_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0262900.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(39648..41072)
                     /locus_tag="IWO09_000278"
     CDS             complement(39648..41072)
                     /locus_tag="IWO09_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0262901.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            41283..41729
                     /gene="nrdI"
                     /locus_tag="IWO09_000279"
     CDS             41283..41729
                     /gene="nrdI"
                     /locus_tag="IWO09_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0262902.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(41794..43776)
                     /locus_tag="IWO09_000280"
     CDS             complement(41794..43776)
                     /locus_tag="IWO09_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0262903.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(43790..44686)
                     /gene="murQ"
                     /locus_tag="IWO09_000281"
     CDS             complement(43790..44686)
                     /gene="murQ"
                     /locus_tag="IWO09_000281"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0262904.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(44689..45750)
                     /locus_tag="IWO09_000282"
     CDS             complement(44689..45750)
                     /locus_tag="IWO09_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0262905.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(45961..46488)
                     /locus_tag="IWO09_000283"
     CDS             complement(45961..46488)
                     /locus_tag="IWO09_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0262906.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(46555..48057)
                     /locus_tag="IWO09_000284"
     CDS             complement(46555..48057)
                     /locus_tag="IWO09_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0262907.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(48315..49436)
                     /locus_tag="IWO09_000285"
     CDS             complement(48315..49436)
                     /locus_tag="IWO09_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0262908.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            49562..50503
                     /locus_tag="IWO09_000286"
     CDS             49562..50503
                     /locus_tag="IWO09_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0262909.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(50653..51549)
                     /locus_tag="IWO09_000287"
     CDS             complement(50653..51549)
                     /locus_tag="IWO09_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0262910.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            51818..53158
                     /locus_tag="IWO09_000288"
     CDS             51818..53158
                     /locus_tag="IWO09_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0262911.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            53376..53819
                     /locus_tag="IWO09_000289"
     CDS             53376..53819
                     /locus_tag="IWO09_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0262912.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(54323..55741)
                     /gene="nhaC"
                     /locus_tag="IWO09_000290"
     CDS             complement(54323..55741)
                     /gene="nhaC"
                     /locus_tag="IWO09_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0262913.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(56144..57367)
                     /locus_tag="IWO09_000291"
     CDS             complement(56144..57367)
                     /locus_tag="IWO09_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0262914.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(57398..58237)
                     /locus_tag="IWO09_000292"
     CDS             complement(57398..58237)
                     /locus_tag="IWO09_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0262915.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(58254..58934)
                     /locus_tag="IWO09_000293"
     CDS             complement(58254..58934)
                     /locus_tag="IWO09_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0262916.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(58936..60009)
                     /locus_tag="IWO09_000294"
     CDS             complement(58936..60009)
                     /locus_tag="IWO09_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0262917.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(60656..61327)
                     /locus_tag="IWO09_000295"
     CDS             complement(60656..61327)
                     /locus_tag="IWO09_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0262918.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(61414..64092)
                     /gene="mgtA"
                     /locus_tag="IWO09_000296"
     CDS             complement(61414..64092)
                     /gene="mgtA"
                     /locus_tag="IWO09_000296"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0262919.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            64751..65188
                     /gene="ndk"
                     /locus_tag="IWO09_000297"
     CDS             64751..65188
                     /gene="ndk"
                     /locus_tag="IWO09_000297"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0262920.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(65210..66076)
                     /locus_tag="IWO09_000298"
     CDS             complement(65210..66076)
                     /locus_tag="IWO09_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0262921.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(66236..68329)
                     /locus_tag="IWO09_000299"
     CDS             complement(66236..68329)
                     /locus_tag="IWO09_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0262922.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        21168 a        13253 c        12995 g        21302 t
ORIGIN      
        1 tttttggtag ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca
       61 acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg
      121 aagaagaaaa acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac
      181 gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac
      241 ttgacattta ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac
      301 gtatcgagca gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt
      361 agcaattcgc aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac
      421 ttgccatcgt actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag
      481 ccgttagtta taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa
      541 tgcaaggagc agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat
      601 atggagatga cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat
      661 tgggtgattc atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag
      721 acagtatcaa acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg
      781 atgaagattt aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc
      841 aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt
      901 tctctaaatt agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag
      961 tgaaatttga ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt
     1021 tgaaagatgg tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg
     1081 aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca
     1141 aaaaagaatt gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata
     1201 aagtaattgg cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa
     1261 atgttttgag tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa
     1321 taaaaaatat gaactattta aatcaataaa actgttcatc taaatctttt tattagaact
     1381 aaatcaacag tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt
     1441 acttagaatc cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat
     1501 caaaaaatgc acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag
     1561 aagttattca cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg
     1621 atcatttgta tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta
     1681 tagcggggat tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt
     1741 ttttcagtta tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga
     1801 agaaacgcta ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta
     1861 ctttattagg tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt
     1921 tcctcagttt catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa
     1981 cagtacttca tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat
     2041 cagaagggaa agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt
     2101 gaacaagcaa aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa
     2161 gtggtcgtct tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct
     2221 acggaagggt atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc
     2281 agtggaatcg ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg
     2341 aatgacttgg ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt
     2401 tcgattgaat cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat
     2461 gtcgtcgcaa ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg
     2521 aaatttggaa aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc
     2581 tactcgtttc acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg
     2641 ataggactaa ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat
     2701 gatggtaagc tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca
     2761 ttgccagacc tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt
     2821 gagcaattag ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat
     2881 gaaggggata aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt
     2941 taaaatggag gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga
     3001 ctgtgacaag acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata
     3061 gaaaaatttt tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta
     3121 aaacgataaa ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt
     3181 aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt
     3241 tgccgatgta gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac
     3301 tactttttca gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac
     3361 taataaaagg caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc
     3421 tgatagcagt gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc
     3481 cacaacagaa gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat
     3541 tcagcctttg actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg
     3601 tggattcgct cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat
     3661 cgagttccag aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga
     3721 tggaaatgcc attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac
     3781 gggagaaata aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat
     3841 ttacgctcct cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc
     3901 agtgaaggaa ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt
     3961 gaatgtgatc aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct
     4021 agattcagga gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg
     4081 ttcaaaggtc tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt
     4141 cgatacgcta cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt
     4201 aaatacaaag atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc
     4261 aggatcgaaa gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg
     4321 cggatttcgc ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc
     4381 ggtcaaaatg gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa
     4441 aggcctttct ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg
     4501 gcgttccagc gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg
     4561 ctcattgagg gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg
     4621 aaatcaagcg atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga
     4681 ttttacagtc actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt
     4741 agatacaact ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat
     4801 ggtcgggaaa acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga
     4861 cttagaatct cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg
     4921 cataggcggc tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga
     4981 aatcgcttta tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta
     5041 tttttctgat atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag
     5101 ccctagtgca ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa
     5161 tcgaatcagt gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc
     5221 agataaaaaa atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga
     5281 tgcttggaca ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc
     5341 atctgtagaa attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata
     5401 taacccatgg ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga
     5461 gagattaaaa acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca
     5521 agaagtgaca gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc
     5581 taatttaagt accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc
     5641 aggagccttg gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga
     5701 tacaggggaa tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga
     5761 aattgtatta aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag
     5821 taccaacgat gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc
     5881 cccgcctttt gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc
     5941 ttccaaactc gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca
     6001 aatggatata caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat
     6061 cttaacgata aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt
     6121 cctatatttc aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt
     6181 cagaggacaa aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg
     6241 ggcgtttaaa cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat
     6301 aacatggaca ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc
     6361 gaatatggaa taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat
     6421 tcatcctttg gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat
     6481 taccggctaa tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga
     6541 aaacacagga aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga
     6601 cattgatcca ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa
     6661 gtaaatttga tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa
     6721 taactgttcc tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag
     6781 agctggctgg tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta
     6841 acaaaagtac agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa
     6901 atagcgaaaa agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg
     6961 atggaagaaa tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct
     7021 tgactacatc ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa
     7081 aagaaaaaat agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa
     7141 aaaaattagc tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct
     7201 ggaatctcca taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg
     7261 atattgattt aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag
     7321 tcattcttat tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat
     7381 gaaaaaaaca attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag
     7441 tgtttttgct gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc
     7501 agatgctgca aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga
     7561 tactcctttt acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca
     7621 agctccagat cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc
     7681 attacaaagc ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt
     7741 aacggataaa agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat
     7801 tggtacaaca tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt
     7861 gattcgaaat agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc
     7921 tgaaggaatc gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga
     7981 agggattggc acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt
     8041 ggaattattt gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg
     8101 gtcgttaaca gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga
     8161 gtggagccga ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt
     8221 catcaactcg aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc
     8281 tttcgaccaa cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca
     8341 attttctgca attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac
     8401 ttttctaact ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt
     8461 gttttgacca taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga
     8521 ggatacagga cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatc
     8581 tgctgttctt tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg
     8641 tcttttctga tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc
     8701 gtataagcgg tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg
     8761 acatcaatac ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta
     8821 tcaatagtgc ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata
     8881 ttctcgatat gatgattgac caattctctg atgatccatt cgctgtgaaa ctggttatct
     8941 tctatgataa aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg
     9001 aaacaaaaca atggaggaat aagatggtcg caacagtgac actttatttg acctttactt
     9061 tgattttaga tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct
     9121 ttggttgtat agctttgatg gtggtgacac ttttacttcc tatccctaaa gtgagctttc
     9181 cttacctagt tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta
     9241 tcagctgttt gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt
     9301 ttgatctgat ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac
     9361 ttatcgcatt tattttctat ctattagtaa aaagtataga caaacagacg agatttctca
     9421 acttgctttt tacttatcca actgaacact attttggagg tatttgtatt ttattaagct
     9481 ggggcgtgat ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc
     9541 tatctctttt gtcgatagtc atcagttttt tttctttgtt tttttccatt atgttgatca
     9601 gtttccaagt aaaagaaaga caacatcagg aatcgcttca actgtatcaa ataaatgaag
     9661 aatattacca tcatctagag gaattcaaac atgattataa aagtctactg ttttcattaa
     9721 aaattactct tcaggaaaat aaaaaagaaa cgttggaatt tttagaaata ttagaagatc
     9781 attctgcagc tatttttgaa aaaccgcaaa tacaacagct aacaaatatt gtatccccag
     9841 ctgtcagggg tgtcttttta aaatttctag aaaaagcaga atcggagaaa atcccgatcc
     9901 aagtagttat tcctgtcgaa gtgaaaacaa tcccgattga tctggttgtt tttattcgct
     9961 gtttatctat ttttatttct aacgcattcg atcatcgtgt agccgatcag agtccgatta
    10021 cgatcaatct gctggaagaa aaagagggat cacgttttag tatagcgaac aaggcaaatc
    10081 caacgaattt gacgctatcg gagatggtca aaagaggaaa aacctctaaa aaaaatggcg
    10141 ggttaggtct atacagtgcg aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa
    10201 tcgaattttc aaaacagaat cactattttt ttgtagaaat gtatctggca tctagaaaga
    10261 gcaaatcagc ctactgactt actgtcaaag gataagtttg aggaaagtct acacggagat
    10321 agagacaaca acacagcata ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc
    10381 tgtttctttt gctatgtagt taaacaaagt agttgacact tccgctactt agtattagta
    10441 cgaaagtata cttcatgata ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc
    10501 aaaatgctaa ctgctttgct tacaccagat aaagggaaga gcaatatcgc ttattttagg
    10561 cgtggagaag caagcaaatc cctacgatca caaaaaaaat gctgacccag cagttaagag
    10621 agttagaaca agatgaaagt atccatcgaa aagcctataa ccaagtcccg cctaaagtag
    10681 agtactcgct caccgaagaa ggaaaatcat taagagagat tctagtagcg ttgtctgttt
    10741 gggaagaaga acgtatcgaa aaacaaaaag cctcaaggga agaagacacg ctactgcata
    10801 cacatggtgg ctacctaaat tattgatttt ctatgacatt tattttaaat aaaagaaaac
    10861 aggagttttt ttaaaagcat catctatttt ttatgtaaaa aaatggtatg attaatcaag
    10921 taagaagtga ttggaagaag gcaagcaaat gaatagtatg gaaaaagtca accagtttag
    10981 agatgaacgt aattggcgac aatttcataa cgaaaaagat ttggctatct ctatttcttt
    11041 ggaagcatct gaattgttag aactttttca atggaaaacg ccggaagaag taaaagaaac
    11101 acaattagaa cgaatcaaag aagaactggc agatgtattg atctattcat acatgatggc
    11161 agacaacctt cacctggatc tagatgagat cattgaagaa aaattgatca aaaataattt
    11221 gaaatatcct gtagaaaaaa gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg
    11281 tgtcattcgg ctgttggaaa gagcaaaaga aaaacagtaa aatgagtctg gagggagaga
    11341 aaatgaaaaa aaaaggaaaa gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt
    11401 tattagtagg actagctttg attttcaatg aacagatcaa ggattatttt gtcagagaga
    11461 caggagataa atatgctata gcgaatgtaa caaaagaaga tctgaaaaag aataacgata
    11521 aagatgtcag ttttgacttt gatgcggtag aaccaatgac gacagaaggg gtcatgaggt
    11581 cgcaaatgag aggaacagat ctaccagtga ttgctagtat cgcagtccct tcagtttcaa
    11641 tcaatttacc ggttttcaaa ggcttggata atacgtcact actttacgga gcaggaacat
    11701 tatctccaga tcaagaaatg ggaaaaggaa attatgcctt agcaagccat cgtgcaacga
    11761 accctgaatt gctgtttaca ccgctagaaa atttagagat gggtgccaag atctatttga
    11821 cagatttaga aaatgtgtat acatacaaaa cattttttaa agaaaaagtt gctcctacag
    11881 atacccaatt actgaatgaa gtcgagggaa aagaaattgt cacactaatt acttgtggcg
    11941 acatggacgc cgtcacgcga ctagttgtac aaggagaact ggaaagtgtt acatcgataa
    12001 aagatgcgac agatgatatg agatctgctt tcaatctaga gacaaaaaca ttttaggata
    12061 ctgggacata agtcgttaca gattaaaaac agccaaagga tatgaatcga taaggaaaaa
    12121 ccaatttgct gaaaaaaaga gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta
    12181 gatagacttg tgttccagtt tttttatttt ttatatgaaa aggcttgcat aaataaaaat
    12241 tcgatggtat gatgattttg aataagaaat ggattgttac tgtacggcag gcaaaaccag
    12301 aattcagcta agaaacagta ggcgaattct aggttttttt atttttcttg agagggcgaa
    12361 gcaaaatgaa aattgataaa attttgaata acaatgtcgt catttcgaaa aatggattcg
    12421 gggaagaagt cgtttgtatg ggaaaaggac tagcttttca aaaaaagatt ggggacgaga
    12481 tttcgccaga agcggtccaa aaagaatttg ttttaagaga ttcttttgct aaaaaccaat
    12541 tccagcagct gatggctgat attccggcag aggagatcga atgggtcaaa caagtagtcg
    12601 aactggctga agataaacta aaagttgaat tttcgcctaa tatttatctg acattgaccg
    12661 atcatcttca ttatgcgatc acacgagcaa aagaaaatat ccaactgcct aatccattat
    12721 tatttgaaac aaaaaaattt tatccgaaag aatatcaagc agcgaaagag gctcttttgt
    12781 tcgtgaaaca aaagacgggt gtggaacttt ctgaggatga agccggtttt attgcctttc
    12841 attttgtgaa tagccaacaa ggaaacgaag acatgcaagt gacgatgacc gctaccacaa
    12901 tggtaagaga tatactgagt atcatcagta aattcttcgg catcgtgttc gatgaagaat
    12961 ctttgaatta tcagcgtatc attactcatc tgcagttttt tgcccagcgt tatctcaaag
    13021 gggaaggatc tgacgagcaa gatgaatttc tctacgcatt agttcaagga aagtatccaa
    13081 aagcgtttca ttgtgtcgaa cggatcaatg agtatcttcg acaaacgcaa aaacaggaaa
    13141 tggggatcgc agagcagatt tatttaacga ttcatattca aagagtagta agtgagaaaa
    13201 aaagcatcca atcataagga ttgttactgt atggcaggca aaacctggat tgaaaaaata
    13261 ggttcacgaa tgtttgtgac tgtttttttc aatctgggtt tttatttttt aaataaaaaa
    13321 gaaagaagga aaaaacaatg gataatcaag cagttggtcg tcgtgtatgg gaagcggtag
    13381 gaggacaaaa gaatgtcaaa agtttagtac attgtgctac gcggttgcga ttcagattaa
    13441 aagatgaatc tttagcagat acgcaaaagt taaaagaaga cccggacgtg atccaagtcg
    13501 tacaaagtgg cggtcagtat caagtagtga ttggcagtaa tgtagcagat gtttatcaat
    13561 cgatcgtgga tgaggaaggt cttgcagttt cggaggatgg aaaagaagag tccaaaaata
    13621 ttttgaatcg tctgatcgat attatctcta gtatatttac accttttcta ggggcgatgg
    13681 cagccgccgg ggtattgaaa ggattcttat cattggcaac ggtgatgggc tggctgacag
    13741 ctgatagcgg tacttatcaa atattgtttg ctgcagcaga tggtgtcttt accttcttac
    13801 ctgtcatgct tgcttttaca gcagctaaaa agttcaaagc caaccagttt ttagcagtag
    13861 caattgctat ggcacttgtg tatccagcta tcacagcagt tgctggtgcg ggagaagcta
    13921 tcagtttctt tgggatccca gtgattcttt caccatctgg ttatacttct tctgtcatac
    13981 caatcattct agccgtatgg gttcaaagta aattagagcc attcgtaaaa aaagtgattc
    14041 cgcaatttct gcaaatgatt ttagttccgc ttgtcgtttt agtagtgatg gttccattaa
    14101 catttcttgc tttaggacca atcgggacgg ttgccggtaa tgctttaggc ggtctcttca
    14161 actcgattta tggatttagt ccaatcgtag caggtctaat catgggaagt ttatggcaag
    14221 tattcgtgat gttcggcatg cattggggat tcgtaccgat catgtttttg aatatcgaac
    14281 aatatgggtt tgatgtattg atgccaatgc ttcttccagc catcttggca caaggtgggg
    14341 ctgctttagc tgtagcattg cgaacaaaag atacgaaact tcgagcatta gggatttctt
    14401 ctacagtgac ttcgttattt ggaatcactg aaccaactgt gtatggtgtg acattacctt
    14461 tgaagaaacc attcattgcc gcttgtattt ctggaggtat tggtggagct attatcggat
    14521 tttccggagt aaaagctttc tccagcagtt tggtcagctt attgacgatt ccgactttca
    14581 ttaatacggt ggatggtgta gagtcaaatg tgacagtagc agtaatagct acaggaattg
    14641 cttttgttct tgcattcgta ggtacattga ttttaggatt tgacgaacaa acacaagaca
    14701 atcaattaga aaacaaacat gcgaatgcag gaaaaccgat tacttcagca cggcatacct
    14761 taaaaagtcc tttaacgggt aaagtactgc cattgagtga agtaccagac caagtttttt
    14821 cttctggcgt gatgggaaaa ggtatcgcta ttgatccaga agtaggagaa ctagttgcac
    14881 ctgcagacgg agagatcacg actatttttc caactggaca tgctgtaggg atcacaacga
    14941 ctgacggagc ggagattttg attcatatcg ggatggatac agtagaatta aatgggaatg
    15001 gctttgagat cttagtcaaa caaggggatc tcgtcaaagc aggagattta ttgattcgtt
    15061 ttgacatcga agcaattaga gcagctggct atagcgtaat cactccagtt gtcattacga
    15121 atactgatgc atttgccgat atacttgaat tagaccaaaa agagattatt gcaaacgaag
    15181 atgttctggc aattgtaaaa taaggaggaa atcaggatgc aatcacgttt ttcagaaaaa
    15241 tttttatggg gtggagcaac tgctgccaaa tcaattagaa ggggcttatg atcgagacgg
    15301 aaaaggacta tccgttgcag atgctatgcc tggaggtaag cagcgctttg cgattatcgg
    15361 cagtgaagaa tttgactgga cgattgatca agaaaaatat atttacccga atcatcgagg
    15421 aatcgatcat tacgatcgtt tcaaagaaga tcttgccttg tttgcggaaa tgggattcaa
    15481 atgttatcgt ttttcgattg cctggacacg aatttttcca aatggagacg aaggtactcc
    15541 taacgaagcg ggattagaat tttatgatca gctgattgat gagtgcttaa aatatgatat
    15601 tgaacctgtg atcacgattt ctcattacga aatgcctctt catttggcaa aagaatacgg
    15661 tggctggaaa aatcgaaaac tgatcgactt ctatgaacga tttgctcaga cagtattaga
    15721 gagatacagc agtaaagtca aatattggat gactttcaat gaaatcaatt cggcctttca
    15781 ctttccagct ttaagtcaag gattagtaaa aagcaatgga gcaggagagt accaaaatat
    15841 tttccaagca tggcataatc aattcgttgc aagtagcaaa gccgtaaaaa tcggccacga
    15901 attgcgttcg gatatccaaa tcggctgcat gattatctat gcaacaactt acagcatcga
    15961 tgccaatcca gtcaatcagg cagcaacaat gatccaaaac caagaattca attttttctg
    16021 cacggatgtc caagtacgtg gagaataccc tgcatacaca gcgcgtaccc ataaaaaata
    16081 tggagtcgat ccggaaaaat tagagcaaac ggaaaaagac ttccagttgc taaaagaata
    16141 tccagtggac tacatcggat ttagttatta tatgtctacg gcaatcaatg agactgatcc
    16201 acaagcagca acttcagaag ggaacttgct gggaggagta aaaaatccgt ttcttgaggc
    16261 aagtgaatgg ggctggcaga tcgatccaga agggctgaga atcgcattga atgaattata
    16321 caatcgttac cagaaaccat tattcattgt tgaaaacggt cttggagcca ttgatcatat
    16381 ggaagaggat ggcacgatta ttgatgatta tcggatcgat tatttacgtc gtcatatcga
    16441 agcgatggct aatgcagtag cagacggtgt agatttgatg ggctatacgc cttggggatg
    16501 tatcgatctt gtcagtgcat caacaggaga aatgagtaaa cgttacggct ttatctatgt
    16561 ggatctagac gatgaaggaa atgggacact gaatcgatca aagaaaaaat cttttgattg
    16621 gtataaagaa gtaattgcct caaatggcgt aaacttgtaa ttaaagcagc tgaatgaaaa
    16681 agaacacact tctcacatgt gatttgtgtt ctttttttat tttttatgtg ctattctttt
    16741 agtaatagta aagcaaaggg tgacgttatg aaaaggacac gtgtactata cttggaagtc
    16801 gcagataaaa ttaaagaaga tattttctca ggaaaatatc cagtcggttc gatgttgccg
    16861 acagaaagtg aattagaaga actgtttcaa gtcagcaaaa tcactgtccg aaaggcaatc
    16921 gaactgctgg catcagatga atatgtcgca aagaaaagtg gtcgtgggac gacggttcta
    16981 agcaatcgtc cttacaatcg attatccaaa gcggcaagct ttacgcaaat cttggagaac
    17041 tcccgatatg aagtccggaa agaaacgctg gatttgcaat tggtccatat aaagaaggaa
    17101 gatcctttgt tttccttttt cggtgagcaa gctgtttgtt ttaagcgctt atattatttg
    17161 aacaatcagc cttatattta ttttgttcat tatcttccac aagaaatgat gcaattcagc
    17221 agagaagaat ttggcaaaga atctttgtat cgtttgctga cgaaaaaagg atatactatt
    17281 gactcatttt ctgatgattt ctcggcagta tttttaacag aagaagaaca aggaattctg
    17341 aaaacagacg cacagatcgg catcaaacgg atacggaaat cgtctacgca aattggggaa
    17401 atgattgaat attcggaggc ggtctaccat acggagctgc atccttacca tattgattat
    17461 gaaacataaa taaaaaaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    17521 ccaaacaaaa atagcttctc atatttttcg tttggttagg cttattaccg cagatacacg
    17581 gctgtttctg gattaccatt tattctggat aagggggtgt ggcacgattt tcagaaggaa
    17641 tctatgaacc aatcactgta ttgattttag cttcgatggt aaccccattc ggcttgacga
    17701 tcgcttattt cttagggaaa atcattcgta aaaatatact caatcgtcaa gaaatcgata
    17761 cattgaagac cgcttttcct atggggatct gtcagatcac agagggatgt ttccctatcg
    17821 tattgaacga cttggcacgc aatgtcatag caacgggagt cggtgggaca gtaggtggcg
    17881 gattaagcat gttttggggc gcagatagcc gtattccggc atcgggaatg tttgcagtcg
    17941 ctacgatgac cagaccatgg gcattcattg gtgcattgtt agctggttct tttgtcacag
    18001 gaataaccat tttattattg aaaaaaccag tagatccgaa tgcagaaatc atccaagaag
    18061 aaaaagagga agaagacata tcttgggatg atttgacaat tagttaggag aggcagaatg
    18121 gattacaaga aaaaagtaca atagatgtac cttgagacag gaatggtatg aaagaatcag
    18181 agttagaaag aatcgaatat acagattttg gtttaggagc gatcgaaact gtccagagca
    18241 tcggcaccct ccttgaaatg acgatgaagg aaaaatgaga acctttcgtt gtcgaaaggg
    18301 tctggtatac cttatgtcga aggaaaggaa acaaaaaaac tttcctgtac tattccaaaa
    18361 aggaaagaaa gttattatac agtaaaaaaa gaaattattc ttcatgctag agatcagtac
    18421 acgttcgagc ccaatatcaa acactagttc caggcaggcg caacaggtgc ggttatcact
    18481 gaattttctt ccagcagtga cgattcaagt gatattttta caaatccagc tatttatcgt
    18541 taacaaaaag atttttagga gaagctgatg aacaaaaatg atcaaatttt gatcgtttag
    18601 ttcgtcggct tttttattta gagaaacatc atttatcagt gaaacataac acgagaacaa
    18661 aatgattttt tcgttaataa aagaaacaag tatttgttgt ttaaaataat acacgttatt
    18721 ggcatatagg acgaaatccc agtatcttct ataagtaagt catttcatat cgatgaaaac
    18781 acgttattta tttttgtttt atctataaaa tggctgaaaa atgatttttt tctaaatact
    18841 atatatagtg gcgtctatcc tatataccac taaaaaacta caaaatctag tcttttattt
    18901 taaaaaaaca cgtgtagaat gaagatagac ttttgagtta gggagtgttt ttttatgaaa
    18961 cttactgttt tttgcggttc ccgttttggt aataaagaaa gttacaaatt tattgcgcaa
    19021 acgttaggaa aatacatggc acaagaagaa atcgaactgg tttacggtgg ttcatactcc
    19081 gggattatgg gagtgattag taaaacagta ctggaaaata acggaaaagt cacaggtatc
    19141 tatccgaatg ggctttttga aactgaattg cctggaaaag atgttactac atttattcct
    19201 acagaaacga tcgatgaaag aaaggtatta ttatttgaaa agggagatgc tgtcctcgtt
    19261 ttccctggag gtctaggtac attggaggaa ttctcacaac ttctttcttg gatagcaatc
    19321 ggtctgacgc ccgacaagcc aatcggaata ttgaatattg gcggttatta cagcggcttg
    19381 caacaattat tagaaacttt tgcaaaagag gattttatgg ataaaaaatg gttggatcaa
    19441 gtaatctttt ccaacaatcc attggaactg gtcaatgaat tacgagcagt atctattaaa
    19501 aatcagttat tattgaagga ggcaaaataa tgagtcaaat atatttagca ggaccatttt
    19561 tttcagagga gcaaatcgat cgagtaagtc gtatcgaaaa agcgttggag gaaaacaaaa
    19621 cagtcacaag tttctactct ccacgtcatc accaagaaag caattatgaa ctattcagcg
    19681 ctggatgggc gcaggaagta tacgaaaaag atatggaaga actgacaaat gcagaatttg
    19741 ttgttgctat ccttgacttt gagcatcaaa caatcgatcc aggaacggct tacgaattag
    19801 gtgtggcaac tatgttgaaa aaaccgatga tcattgtaca agaagagacg gtacctacga
    19861 acttgatgat tacacaaagt ctccatacgt atttaaaatc agaccaagct gtacgtgaat
    19921 atgattttga aacattgcct gttgaaactt acgtaggcga gtacttatag aagaggaagg
    19981 gttgtatgtc agtgattttg atttcaggaa cgattggtgc aggtaagagc agtttgacgg
    20041 atatgcttgc aaaagagatt gattctaaac cattttatga aaatgtcgaa gataacgaag
    20101 tattgccgct cttttattca aatcctgagc agtacgcttt tttattgcaa atcttctttt
    20161 tgaacaagcg atttttagca atgaaaaatg ctttagtgaa cgatgacaat gtgctagacc
    20221 gatccatcta tgaagatagc ctgttgttcc atctaaacgc tgatttaggt cgggtaacgg
    20281 atatcgaagt acagcagtac gatagcttat tagatacgat gctaaacgag ttagatgatg
    20341 ttgctccaaa aaaacggcct gatttgatgg tccatatcaa agtttctttg gacacgatgc
    20401 tagaacgtat caaaaaacgt gggcgtgact atgaacagct agaaagcgat gagacgttat
    20461 atacctatta tgaaacgttg aacacccgct ataatcaatg gtatgaagat tttgatgtat
    20521 gtcctaaaat acaagtggat ggggataaat tcgattttgt agaaaatcca gaagatgcaa
    20581 aaaaggtgat ccgtcagatc caagaaaaat tagctgaatt ggaaggaaag cctgctggaa
    20641 aatattttac ggataaccag catgcaaaag gtcaagaatt aggctaaaaa taaaaaacca
    20701 aacgacggga aagaaaaatt actacgtgat gtagaataag cctttctatc gtttggttat
    20761 tttatattca aatatttgaa ataaataaat ctttactctt tgatttgtaa actgaaatga
    20821 tccactgttt gttttactga tgaagcttga tcaaaggaag aagctgagtt tgaagttcat
    20881 aaaaatcagc catagtgagt tttgcttgga tttgtttatc ttcttctact ttttacataa
    20941 ttttgaacag gataagattc aaatgtttta aatgaatctt tgactagttt gtgacagttg
    21001 tcgcatatgc tcaagcttcc ttggtcatta aattctgtat ggatattttt gttgttatta
    21061 catgtacaat aatcaggact atcgatcatc catttcgtct cctttacaat catcttgctt
    21121 ctttactata gcagaaaaga aggggagaaa caaaagaaga tccaacagac ggcactatag
    21181 actgtagatg ttgccatcct catccttcaa acggtaatta ccgatttttg taaatatgac
    21241 tgttgtttct tttttatttt cttttcctga aataatatat tcgtcatctt tggtagtgat
    21301 cttgatatct gtcagcactg ttttttcttc agatgattca gaagaatcag ttccttgtgt
    21361 ctgatacgtg cgtgtccatt ccattttttt atctgtaaat gtcaactgtc ccgcggataa
    21421 atccatatct ttacgtactt ttagcgtata tacactgttg gtggtatctg ctttttccgc
    21481 ttttaagaca gaagaatcgg ttgtttcgtc cgaggattgg tcagagccgc caaacaaatc
    21541 acagccagtc aatgtcaaaa gtcccgcaga caaaataaat agtacgatta atttcttcaa
    21601 aatacttccc ccatttgata ggataaagaa attataccat tggaatgtaa aacttcaaac
    21661 ggatggaaga gaaaaacgca caaaacaccc acaaatagag taaattttag aataaaattt
    21721 taaaaaggca aaaaatccgc cactttcaga aagcgtttta aaaaagtgtt gcaatctttt
    21781 aaaaaaatgc tatattaaaa atgaaccggt tcaaatactt gtttaagtat aattaatagt
    21841 gagaggaagt tgctctactg aagactttag ttatatggat attgatgaca agcaacacta
    21901 tgcaaagaat gcggtcatca taatcgaaca acaagaaaga gaaatcaaat gtaactggca
    21961 ggagtgagaa tgtatggcat cgatccgtga tgtggctaag atggctggtg tttcagtggg
    22021 aacagtttcc cgttacttga atggtcagca actgaaggaa aagaacatga aaaaaatagc
    22081 agaagcgatt tcagcgttgg actataaaga aaacatcatt gctaaagggt tgaagaataa
    22141 tcgaagcttc tctattggac ttttgataaa cgggatttcc agccgttttg ggtcggaagt
    22201 tgtctcaggg atcgaaagag tagctgaaga aaatgggtat agcttattat taagcgggtt
    22261 tgccgatcag ccagaaaaaa ttgaacaaaa aattgaatac ctgatgaagc atgtgatcga
    22321 tgggctgatt gtcttcttat ccgaagaaga gtggtctgga tttgaaatgc tcacaaaaat
    22381 gagtattcca gttatcgcat tgaattgtcc agaaggacca gcaggtattg atacaatatt
    22441 agtgaatgat cgagaaagtg tagcaaaagt gatcgctcat catggtgaac aaggacataa
    22501 aaaaatcgga tttattgctg ccactcaaac agactacgta gcaagagaac gtttagcagg
    22561 agccaaagaa gcggtccaaa atgatccatc gatccaatta gaggtcttta caggagacta
    22621 ttctagaaaa agcggctatt acggggcaaa agcattatta gaaaaagggg caactgctat
    22681 ttttgtcagc aattacaata tgtcgattgg tgccatcgaa tgtttcaatc aagaaggtgt
    22741 cagaatcggg aaggatattg cgttcagtca ctatgattat ttagataaaa acgatctgag
    22801 tatccttccg aaaatcacga tcacgccacc aacagataaa atcggggaac gagcagcaga
    22861 acagctgtta aaacgaatca aaaaagaaga ggaagaaaat gaaaaagtga ttgtgatgga
    22921 aaatatcata catggaatca actaaaacga actaaaaaag aaaaagaggt gctttgatga
    22981 aaaaatatca atttccagca aatttttggt ggggttctgc agcaagtggc ccacaaacag
    23041 aaggaaggat ttcaggcgat ggaaaaggag agaacatttt tgattattgg tacaaaaaag
    23101 aaccgcaaaa gttctttgat caagtaggac ctgaaaaaac ctcgcaagtt tatacgaagt
    23161 atcaagagga tgtacagttg atgaaacaga ctgggcataa ttcatttcga acatctatcc
    23221 aatggagccg tttgatacct gatgaatctg gaacaatcaa cgaagaagcc gttcgtttct
    23281 atcatcgtta ttttgatgag ttgattgaaa acggtatcga accctttgtg aatctgtatc
    23341 atttcgatat gccgatgtat ctgcaagaaa aaggcggatg gttgaaccga gaaacggttg
    23401 aagcctatga aaaatatgcc aaaagttgtt ttgaattgtt tggttcgaaa gtaaaaaaat
    23461 ggtttactca taatgaacca atcgttccag tcgaagcagg ttatttatat cgatggcatt
    23521 atccagaaga gtcagatatg aaaaaagcga tccaagttgg ctatcatgaa gcgttggctt
    23581 cagcgttagc catcaaatct taccatgaaa tggagcaagg tggtgttatt ggtatcatct
    23641 tgaatctgac accaagctat ccaagagacg aatcaaaccc tgaagatgtt aaagcagcgc
    23701 aaatagcaga tgcgttcttc aatcgttcat tccttgatcc agctgtaaaa ggcgagtttc
    23761 caagtgaatt gattgagata gtaaaagaac ttgatctcat gcctgtgatc catgcagagg
    23821 atttgacggt tatcaaagaa aatacagtag atcttttagg tatcaattat taccagccac
    23881 gccgggtaaa agcaaaagaa acaccaatcg atactaaaaa cggtccaatg cctgaggatt
    23941 atttcgacaa ttatgaaatg cctggtcgta aaatgaatcc ttatcgcgga tgggagatct
    24001 atgaacaagg aatctatgat attttgatca atgtacgaga tcattatgga aatatcgact
    24061 gctttatttc tgaaaacggt atgggtgtag aaaacgaagc acgatttatc aaagaagatg
    24121 gcatgatcca cgatgattat cggatcgaat ttgtccaatc tcatctgaaa tatgtccatc
    24181 aagccattca agaaggtgcc aattgtttag gctatcatat gtggacatgt atggataact
    24241 ggtcgtggac caatgcatat aagaatcgat atggctttat ttcagtagac ttagcacaag
    24301 atggaaaaag aacaatcaaa aaatcgggtc attggttcaa agacgtatca gataaaaacg
    24361 gatttgaggc ttaagaaaga atagttgaga gcaaatgcgt gaaattctcc attcttacca
    24421 ttttgtgaaa aagaagttca attatttacg atctttttca acttttttgt ctttctattt
    24481 cattttttgt atataataga atagtcaaaa ttaaatagga gtggaggaaa gcatgaggaa
    24541 aaaaagaccg tttcttctct tttggttatt cggttttttc ttactgctta ttttatccgc
    24601 agcattttca ggaaaaatga cagcagatct tgctcagaaa gaaacagaaa aaatcgtagc
    24661 aagcaaagaa tcagtagata atttcaaaaa agaattctca acttattggt gggcggagta
    24721 taaaataact gccaattacg aaaaaggcga attggacatt tatttgccaa aaggaaaaga
    24781 agaaaatcaa tttattaata tcgatgacgt acttagtgcg atttctctca gacaatatca
    24841 atttaaagac aagcatttga ccgttttgct tttgactcat gatggcgaca aagtagcccg
    24901 tttgaaaaat ggaaaagtga tcaccgctta ttctgaaaaa gtagacaaaa aagtggtcgc
    24961 cgatcgttta gcggaatgga aaaagacaaa tgaacaagtc aagaaaacaa ctgtctcttc
    25021 tcaaaaaaac gagtcgacag gaacaagtcc ttcagattct ggcagtggac aaacgataac
    25081 gggaagtgaa gaaaatcaag cctcaattat tacgaatgag tcatcacaag gacaatatca
    25141 atcctatgga caatgaaaaa cagaggttgt gaaatcagcg ttttgacctg agtattagag
    25201 aaacaaacgg caaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggtca
    25261 gctgattgaa caccgtttat tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc
    25321 cttcgtgtgt ttttctgcct tctcaaaagg atgacagttt ctcactcttt tcctatttct
    25381 tctattcaat caaataactc attttatata catctaaaaa ttcacctaga tctgttcttg
    25441 cccctctagg catgaccgct tcgatttgga agcccatttt tcggtaaagg tgtacggctc
    25501 tttcattacg aacttgaacg tcaagctcca atcgaaaaag tacacccatt tccttcgccc
    25561 aactgatgat ttcttctagc ataagagtgc ctagtcccat tccccaatat tcctgcagaa
    25621 tactgatacc gacttctccc acgtgagaca aacgaaattg actgtctgcc ttgacagatg
    25681 ctgttccaat aatcgtccct tcatagattg caagcagcaa taaattatta ttgctttcgt
    25741 atatataatc caactcttct tctaaggttt ctggtggtaa caggagttct gcttcatcca
    25801 aaactaaaaa ttctgtctcc ttattaactt gtcccatcat caaaagaatg tcttttgcat
    25861 ctcctggcac tgcttcccgc aagatgatcc caacttcttc actcatgatt ttcagcccat
    25921 ttttctagaa ttcgctgttt gaaggtttca ctttgctccc cgtatgagat tagtttcaca
    25981 taccgctttt ctaaatctgt atcacttttt tctaaaatca gttccaaata atcctccggc
    26041 agtgcttctt ctaatagatt tccccattca acaacggaaa gcccatcacc ttcaaaatat
    26101 tcatctaacc ctaaatctgc tccgctagct gctacccgat aaatatccat atgatacaac
    26161 ggaatccgac cttgactata ttctctaata atcgtgtatg tcggactttt gatcatttgc
    26221 gttatcccta atccttgggc aattcctttc gtcaacgttg ttttacccgc acctagatca
    26281 ccagtcaata tcagattgtc acctggcatt gcggaatgac caattgcctc tgcgaattgt
    26341 tctgtcattg ttaatcctga tagttcgatc atttttcatc ttctcccgct cttctttctt
    26401 tttgccagta tatcgcgtaa tgaagttttt ttaaagaaaa aatgaactag tcaagaacag
    26461 cagctgctgt ttttggctag ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg
    26521 tgataatcga taatttatat acatcttctt catttgctcc acgagacaaa tcagaaaccg
    26581 gtttgttcaa gccttgtaga attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa
    26641 ttttatagcc gatatttcct gactgcaatt ctggaaatac aaagactgtt gcttgaccag
    26701 ctacttctga atctggcgct ttcagttgtg caactgaagg aacataagaa gcatcaaatt
    26761 gcaattcgcc gtcgatcatg tattgtggcg ctagtttttt agcgatttta gttgcttcaa
    26821 ctactttgtc tacttctggc gatttcgctg agccttttgt cgagaagctc atcattgcta
    26881 cttttggttc gatatcgaac aattctgctg tttttgcaga atctacggcg atttcagcca
    26941 attcttgcgc agttggattc acgttgatcg cacaatccga aaagacatat ttttcttgat
    27001 ctcttccacg taccatgatc atcgctccgc ttgtccgact gatgccaggc tttgttttga
    27061 tgatctgaag tgctgggcga acagtgtcgc cagtcgaatg gatcgctcca gagaccattc
    27121 cgtctgcaat ccccatatac gtaagcatcg taccaaagta attcacatct tttaaaatct
    27181 tttctgcttg ttctttggat gcctttccat ttctacgttc aacaaaagta tccaccattt
    27241 catcccactt atcgtaatta tcagggctga tgatcgtaaa gctagatgtt ttgattcctc
    27301 tggcatgcgc tgcatttctt atttcttctt cgttccctac taaaatgggt tccacaagtt
    27361 cttctgcttt caaccgggca gctgcgccta aaatacgagc atcagtagct tccgggaaaa
    27421 cgattttgat cccacggcga acaattttaa atcttaaact atcaaacaat tccacaagat
    27481 gacctccaga cgtttttata tgtattatca tacactattc cagaaaaaaa gaacagttta
    27541 ttttatctgt atcagtcaaa aatgctggaa aaattaagtt tttcacatac aaagcgaact
    27601 gcactataac aggaaagcgc tgttttatta cactgtttca ggcagttgcc aatcaatcgg
    27661 ttcttctcct aacgcaagga gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa
    27721 gccacgactt gcagataacg gactgggatg tggagatgtt atgatgatat ggcgactctt
    27781 atcgatcatt ttgatttttt cttgtgccgg ttttccccac aagataaaca cgattggttt
    27841 ttcccgttca tttaattttt caatgatcgt gtctgttaat ctttcccaac ctttcccgcg
    27901 atgagaatac gcttgccctt cccgaacggt aagaacggtg ttgagtaaaa ggactccctg
    27961 tttagcccat gacactagat ttccatgttg aacaggtgcg attccaagat catcataaag
    28021 ctctttatag atatttctta acgatggcgg gatctttacg ccaggttgca cagaaaaaga
    28081 taagccatga gcttgatttt ctccatgata aggatcttgt cctaaaatca ccactttaac
    28141 ttcttcataa ggagtcaatt ccaatgcttc atagatatga tacatatctg gatggatctt
    28201 ttgggacgca tattcttttt ttaaaaattc tcttagatgc agataataat cttttgaaaa
    28261 ttctgaggac aaaacttctt gccaactgtt atgaatgatt tctttcaaaa actccacctc
    28321 ctaataaaaa gaataactca cattttcttc aatcacaagg gacaatcttc accctcagca
    28381 cttgattaat atggtaaact aggaacaaga agcagaaagg aacgataatc attatgatca
    28441 aattaattgc atcagatatg gacggcacat tattagatgc ccatatgagt gtttctcctg
    28501 aaaatgccga ggcgatacgt tttgcaaatg atgccggtgt tgagttcatg gtcgctactg
    28561 gtcgaaacta tcaagaagct cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga
    28621 tcactttgaa cggggcacag gtatttgata aagaaggaaa ttcattgttt accgtgtcga
    28681 ttccaaataa tcaagcaatc tctgttttag atattctaga tgctaacggt atttactatg
    28741 aagtagcaac caacgatggt ctttattctg aaaatcaagc gaaacgaatt gaaagttttg
    28801 cttccatggt ggctacccat ttacctcacc ttacgtataa aatggcgatt gccatggcct
    28861 cagctaagct ggagttattg cacatcacat acgtggacag tattcgtgct attttggaag
    28921 aaaagaatct ggaagtttta aaaattattt gttttcatac agaagggcca gctgtattag
    28981 gtccagttgg aaagcagatt tctcttttag gcgatctagc cgttacttcc tctggtcaaa
    29041 acaatatcga agtgaaccat aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc
    29101 acgagcgtgg gatttcttta gaagaagtca tgacgattgg cgataacttc aatgatgtca
    29161 gcatgctgca agtagcagga gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag
    29221 attatgccaa atatacaacg gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc
    29281 gcgcgattgc agaagacttg tagttttatc tagaaaagag ggatatagat gtcagaattt
    29341 ttcattcagg aaaagcagct tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga
    29401 cattccctgt ttttgatggt tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat
    29461 gccatgaatg gcgatctcgt tgctcacatc aagcagataa gcctgacatt cggtacgcgt
    29521 tttgagctgt ataaagagtt cgaaaaggtg ggaaccatgc agaaaatatt caattggccc
    29581 ggtgactttt attatattcg tagacttcac tggacagtcc aaggtgatat ctataatcat
    29641 caatatcaaa tccatcattt caatcagcaa atcatgaaaa tggataaagc aacacttttt
    29701 tcaggagatt actatgtatt ggatgtctca aatgatgaag atgctccagt ttgtatttgt
    29761 attgctgcgg ttttagatta ctggctctac cgtaaagaca aaagtcgcga taagcctgtt
    29821 tcttaccgac ttaacttaag ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag
    29881 gtacatcttt ttagatattc ttttgcatat gatcataaag atgaccactg atcatccgct
    29941 gaccgacaac tttgaagtca tggcgttcgt ataatttttt agcctttgga tttttttcat
    30001 ccacacttag accgatgacg gagcgattag ctttcttcgc caatttaggt aatgcttcta
    30061 ataatcgcga accgatgcct tgtcctctaa aatcttctga tacactgata gagtccaaat
    30121 accattcgtt gggaaacgtt tctttatcaa tgaacaaacg tgtagacgca tctagctgat
    30181 atttctctaa gataggtacc agtggctgat cgatgattga ttcttcttca tctgtataac
    30241 cgtaagcaac tcctgcaact ttaccgtcta tttcttctac gatacctcgt gaataactgt
    30301 aacgataatt tggatcagtg attgcttctt ctaaaatctc taaagtcttt tgttcccctg
    30361 tctgcttcaa aaatggcagt tccatatctt ttagaatcac taagatcaat ggggcgattt
    30421 ctggagcatc ttcttttctc gcaaaacgaa tcatagtttc ctctcctttt ctccattcta
    30481 acatgcagaa aaagaaatcg caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa
    30541 tgaaataacc atgagtaacg attcttgccg cttcagttcg gcaataaggg cagacgactg
    30601 gcatttcttt atcattatgt tcgactacat tattgacttc ttcgtatgca aatttttttc
    30661 cgcaattagg gcatttcatt ttttccatgt tatctcaact tcctttcatc tttactctct
    30721 tagcttactc ttatattgta aaaaagagaa gcaatatcct tcttctatgc agatatccat
    30781 acctgacggt cctgttctcc atctattaat cggactttat aataaggacg ttcatctttg
    30841 actagaagtg tccactcttt cacttttgct tttttcccgt agtagctgac tgcttccttc
    30901 atcgctgctt gcggttgttt gaactcttcc gttttttcta tatcaaaaag atgttctgcg
    30961 gccttttccg tgtcatttcg tacggttgtt ttcccagtgg atggattgac tgctatgact
    31021 tgtccttcac aaaaaaactg gtattcatat gttgcattcg taggattctc caaatctttt
    31081 tcaactgcaa aatgaatagc agatatgtta tgttcttttg tttcgttttt tacgattgct
    31141 gctgcttttt tcaatgagat ccgatattcc tggtcactaa tatcatccat gtgaaaagcg
    31201 gcattgagca gtcccgtcat accggtaatc cctattacga tgagggcagc aacaccaaac
    31261 atcatttttt tcattcttca ttctcctgtt tctttttttt aaagaatatc aagaaatgaa
    31321 cgaatgaaaa atcgaatgaa acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca
    31381 gttctaatgg caatccgtct ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat
    31441 cttctctaat cggttctgat tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa
    31501 tatcgtctac tttgaaagca agatgacgca agccacaagc ttctggatag tttggtcgtt
    31561 gtggcgcatt agcaattcca aaaatctcca attcagcact tcctagtttc agatctaatt
    31621 tatagtcatc tcgttctttg cggtaatttt ctctgattac ttcaaatcct aatttattaa
    31681 cataaaaatc acgtgatttc tgataatctg aaacaatgat ggctatatga tggatcgttg
    31741 taaggttcat atgagcactc ctattcttcg tttaagctcg gatctatatt aatctccagt
    31801 tccattgtaa atgtgtcaga ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag
    31861 gcaaaaaaaa cagaaagtag gtcctccaag acactcttcc tgttcccaat ataataaatg
    31921 aaagtaagaa attatatttt caagaaaaca aacagaaaat tatcataaca caaattattt
    31981 ataattgaaa gactagtttt ttttcaagaa aagcatacaa tagattgtgt tagtaaatac
    32041 agaacacact acatttagtg tcttaaggag tcctcataca tgaagtcata ttttcagttt
    32101 ttagctcatc agctaaaagg ctggccgcaa caaaattatt atcttttctt tttcagtctt
    32161 ggctgccagg tcatgactct tgtcaatagt cccatcactt ggctgtccgt tattacattt
    32221 atcggtacca cgcttggtgt attgtgtatt ttatccatca atgcggcaaa atcagtcaac
    32281 ggtattttag gtattttgtc tgcgatttgt tttattatcg ttgggttttc agcaaagaac
    32341 tatttaagta tcggtgaaca gattgcttat atgatcacat tggatatccc cgtattgtta
    32401 agtaaagaat ggaatcataa tatggcttca aaaatccgaa aattcactgg aaaaagctgg
    32461 tttatcgcaa tcatcagtac tttccttgtc tatcttgttt caggattcct gattggtcgt
    32521 tatacagatg atcctcggcc gtgggtagat gccattgctt tttctattag cttgactgct
    32581 ggtatcatca gtttcttgcg ttacaacaac caatattact ggtggttagc ttcagggctt
    32641 gctcaaatgg tcctttggta tatttcattt acgcatggat ctgcaagttt ggcgatgttc
    32701 gtgaacagct ctgtttactt gctgaatgat gtccttgcat ttaccgtttc accttggtat
    32761 aaccaaaaag aacgtgaacg tatgcaaaaa atggaagaag aatactacaa cgcacaacaa
    32821 cacgcataaa aaaactgagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
    32881 acacgtataa acggcggaat cagaagcaac tccttcggaa ataagctgaa attcacaaaa
    32941 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
    33001 agcctctatt ttttatagta attccttcag atactttctt gcctcatcat tgaaacgcag
    33061 tgattcttct aacttctgat aatattccaa cgcttgtttc tgagcttcag taaaacgata
    33121 atgttttcct ttccatttgt cgattgtctc ttgcacattt ccagattgga tatctttcac
    33181 tactagttgc catttttctt ttgagactgt acggtctttc ttatcagcag attgtatagc
    33241 ttctcgttct ttacgcaata cttttaaaaa accatttctg actatccgag gaatgctgtc
    33301 gaagaaccct tctgtaaaac catagcgaca atattgcttc caaataaata gatagatttt
    33361 tggttggatg aatcgttggt ttttcgcaaa atattccatc tgagcagtga cttctggtgt
    33421 tcgttgacgt tttgtctttc tcaacacgga aataaatgtt tcaaaataag cattaggatg
    33481 tactggcact tcttcatata cgatggattg ccaatcttta cgtaaaaatg cgcgatagtt
    33541 gatcaagaat tcttctacat cctttactaa ttcttctgca aagtcgtcaa atagttcttt
    33601 ttttatctgt cgcttttttg actgcccatt gatgtgttca cttattttct tgatttcatt
    33661 ttccaatgct tgataatccg ctatgtaaat cggcatttct acttggcgaa attctgcgag
    33721 ccgctgagct aattttatat ccggtaaata tggctgtttc atccgacgat tttggtacaa
    33781 gacaaaacag tatttttgcc atagtccttc aggaaaatcg atattcttct gcttaagcca
    33841 taataattcg tctaaggcca gatcaacttt tgtcattccc tgatggatcg aagctgccac
    33901 tgtcggagag acatgcaggt gatcggcaaa gtggttgatg cctagtttta ggatctttcc
    33961 ggtttttggc gatttaacga tgtattgcac ttttaattct ctaccacact cacaatacaa
    34021 tttttgctgg cttcgttttg ttcgaaaaaa cggattgatc ttgaagtcag aaaacaccca
    34081 atctgttgca gctaaataat gttcttccat aaaacgagag ctgattagat atcggcggtg
    34141 cttatcgact agttcccgct gttttttcgt taattttgca tagatctccc aacgttgact
    34201 gcttgtgtat tgtttagaaa ttttgatggg atagcttttg ctgatttctt ttggaaacca
    34261 ttcttcaaag acggattgcc attcttccac ggctgtttct ttcctttcta attacaaaaa
    34321 gctggctact aacataacgt tagcaaccag ccagtgtttc ttgctaaatt agtgagttgt
    34381 tccggcattt ccggaaatat cttctttatt ttctactgct gcttcaactg cataagtcaa
    34441 agaagaagca atgtcttcta aagacataga aggcgtaccc actggacgat cgatcacttg
    34501 atcgggtgag aatggcacgt ggatgaaccc acctcgaatt gccgggaatt gtgtttcgat
    34561 caaatgaagc aaacgataca tgatatcatt gcagacaaat gttcctgctg tataagagat
    34621 gtacgctggt agaccatgat cttgtacatt ttttgtcatt gctttgattg gcaatgaggt
    34681 aaagtaagcg gctggaccgt tttcttctaa agccgtatcg aatggctgat ttccttcatt
    34741 atcagggatt cttgcttctg ctagattgat tgcgacacgt tcgaacgaga ctgctgaccg
    34801 cccacctgct tgtccgacac agatcacgac atctggttgg tgctgttgga tggcttcttt
    34861 gactacttga ctgctttttt cgaatacggt cggtacttcc actttgatga tttcagcacc
    34921 gctgatttca tctggcattt tttttactgc ttcataggct ggattcactt tgtccccacc
    34981 aaatggatca aaacctgtta ctagtacttt catctgttgt tcctccgtcc gattgattta
    35041 acatatggtt atcttttatc ataccatttt ccttgaacag cgtaaataaa ggaaacaata
    35101 gtttaaataa cataacagat caaacttaat atattcttat aaaaaaatag tgttccacta
    35161 caatgttaga agaggttgcg acaattattc tgtcacaacc cctctcttct attatttaaa
    35221 tagaatcata taagcaattt ggaaaactag cataaataac gcaataaaca actggttttt
    35281 gatcactccg tatttgtctt tcatctccag catcgctaca ggcacgatat tgaagttagc
    35341 agccatcggt gtcagcaggg tcccacaaaa accgcaagtc aatgcaacca ttcctattaa
    35401 cgctggattc gctccatgac taaatacgaa tggtgcaccg atcccaaccg tcatcacggt
    35461 gatggccgca aatgcattcc ccatgattac cgtaaatatc accatgccaa gagcgtaaat
    35521 gatgatgccg atgttcacat ttccttctgg aacgatcgta cctactgcat tggaaatcac
    35581 tgtcccaact ccagcgcttg taaataccgc acctagagaa gctaaaagca ttggcaacat
    35641 gcttaacggc cctactgtgc ccagcatatc tgctgcatca tctaaaaatg tacttggtcg
    35701 attatctcga gaaaagaaca tcaagattag catggctgca aaaacaccga cgcccacgcc
    35761 gactaaagca cctaaattgg taaatagagc aaacaagatc gcaaagacac cgatactcaa
    35821 agctggaata aaaattttca ttcctagttt gtctgacatt ttttccatat attcttttga
    35881 tggaagccgg ctttctcctt tgctgacttt cttagcaatt gctggtatgg tcatcgcaaa
    35941 gatcaagcca ccgttcacga ttgccggaag ccatcttcct cctgcaataa ctacccctaa
    36001 gaccgtccag aaaaaggctg acccataacg atgaggattg gttttatctg ttaaatttct
    36061 tactccagtg taaatcaata cgagtcccat cacgatataa aggatctcca gtaatttatc
    36121 gcctaataaa atatcaggat ttgtaaaaaa gtccatctta ttgccccttt cctggataat
    36181 atttcttcgc taatttcttt tctttaagat agaagtagac acttgccaca actaaagccg
    36241 tgatagccac tggaacttcc actaaagcaa gctgtcccaa tgttacgtca tatcctagat
    36301 ctttcagtgt tgattggact agcaacgccc ctgctcctcc gatgaatagt acttggccaa
    36361 agaaccagca gacattttcc atagctgaag ccataccttt tagttcttct tcatgcttag
    36421 gatggacttc taaccctttt gattcgatgg tccccaatgc catcggtaaa atgattggac
    36481 gaacaaatcc agcaactcca ccaaaactca cgttaaaagc cgcaaagatc attcgaaaga
    36541 tcccataaat atctacaatc ataccagcag atactttctt gaatcgcttg atcaaggaag
    36601 ccgctgattc tttcagacca tttctttcaa gcgttcctgt tgccaacata atgatgataa
    36661 aaatcgccat cccgcgattg tttacaaaac ttgtacctaa aagttctaac attccttgaa
    36721 tgccaagacc gccagtcaaa gctgtaacaa ctaaagcaac cattacgatc aatatcgagt
    36781 ctaatttaaa tgcaaacccg agaataacaa tcaagacgcc taaaaggcta agataatttt
    36841 ccatatattt tcctccataa aatgacaata tttttttaat tataaagcaa aacacataaa
    36901 aaatatagtt gttttctcac gaaatagaca ctatttttaa atatttttcg ttattttttt
    36961 atcacaaaaa ttatcttact tattgtaagg taaagctttt tttaatttat tgcccgataa
    37021 tatatagaaa taaggtctag ttactgtttt ttttaaatga ttaataaata acaacttctt
    37081 tctatgaaat tgcaaagcat tcatcttctt ttccgctaac gatatatccg tcagtgcttg
    37141 gtacacttta ctaaaaagta tgagattgcc gatatctgga taggtgtgct atactacaga
    37201 aaaagatata caatctgcca ccgggtagga taaagaggat gatcattcca ttaggtcttt
    37261 gaagggatga ttttcctctt tttgtgtatt tttttatagt taaggaggaa tgtaagtatg
    37321 gcatggatgg aattaatcat agcaggaatt ttggaagtat tctggtcaac gatgatgaaa
    37381 tggagtgatg gtttttctaa aatcaattat tctttttaca cagtgattgg catgattgct
    37441 agtttttact ttttgtcaaa ggcaatcaaa agtttgccga tgagtctctc ttatcctatc
    37501 tggacaggga ttggcgcagt tggttcggtc ataattggtg tggtcttgtt tcatgataag
    37561 ctgaatcttt ccacttggtt ctttgtgggg atgctactca tcagtatcat tggcatcaag
    37621 attactagcg gccactagac acttggaata agtactgatc agaaaaaata agccttttta
    37681 tttaatattc aattttttta tgaaaaaata agaaataagt ccaatgacct agcgacttct
    37741 ccactcatca ggtgtatgct tgtactatct atagaaaagg agtgaatatt tttgaaaagt
    37801 ctttcggtcc aagaattatc caagaacttt ggtcacaaac aagccctcag cgatgtcagt
    37861 tttagcttat cccctggcaa aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca
    37921 acgatcatga aagctatttt aggactgatc cattactcag atggaacaat ctgtattggt
    37981 gaaaatgaga tttctccctc tagtcataaa gggttggaac aagtaggagc attgatcgaa
    38041 tacccgggta tctatccatt cttaacaggc tatgatcatc tgaagttatt ctctgaaacc
    38101 aacgatgtat ctgctatcga cactatcgtg aatcagctca agatggaaaa gtacattcac
    38161 aaaaaagcga aatcctactc gttgggaatg aaacaaaaat tgggaattgc tcttgcctta
    38221 ctgaaccatc cgtctatcgt tattttagat gagccgatga acggtttgga tccacaagca
    38281 acaagagatg tgcgaaaaat cattaccgac ttggctactc agggcacctc tttcttgatc
    38341 tccagtcact tactcagcga gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa
    38401 ggaaagatca tcaaacaatg tacaatggct gaattgaacc aaacagcgca agatttcatc
    38461 gttatctcta caacaaatga tctagctgct cgaacgatct tgacagaagc tggatatact
    38521 ttggaagatc ctgataaaat caagctttta aaagaaaacg aagaattatt aggaaccgtg
    38581 atccagctat tgtctgcaca aaatatccat gtgacagacg tccagcatct gacgaatgat
    38641 ttggaatctt ctctattgga aattctggat tcagtaaagg gggaagaaga aaaatgacat
    38701 tgcttgtaaa acaagaatta tttaaattga tcaagaaaaa gtcaactgct gttttatctg
    38761 ttctattagt tgttttgctc ataggaacag cacttctagc caaaaaatac acgacgatca
    38821 ttgatccagt tgaaatgact gctcagcttt ttagtgccac atcttggatt gtctttatca
    38881 tgattgcagc tgcaagcacg atcatttcga tggaagcaca atatggtaca ttgaaaaatc
    38941 tcctttatcg aaagtattct cgaggagaaa tattagtaag caaatggatc acattagtta
    39001 tttattctgt ttatttatac cttttagcta ttattgtcac tgtgttgatg aaactgattc
    39061 ttttcccttc gatttccttc acggagaagg tttctactgg tcaaacactg atccaaagct
    39121 tgttcactta tactttgggt agttatatcg gtttatggct gattttaagt ttagtgttga
    39181 tgattgcttg ctttatcaat agttccggcg catccatctc agcaggaatc gtcttttatt
    39241 ttgcttcgtc aatcatatct ggtatcctta ttgcactcat ccaaaaatgg gagtggatca
    39301 aatggaatcc gatcagtatg ttgaatctgc aaaaccaaat tggaaacgaa gagatcatga
    39361 agcaattgac tcatctttct actaatcaaa tgctattcgg taatttagct tacatcgttt
    39421 tgttcttagc attaggatat ctcgtattta agcgaaaaaa tgtatgatga ctcatcaaaa
    39481 aagaggctgt gaaagagacg ttcagcctta ttgccgattg gctgtctctt aaacaccgtt
    39541 tattctgaaa aaggagctgt gacaacattt gtgtcacagc tcctttccta gtatgttttt
    39601 gaataaacga caggctcaca acctctcttt atacttcttt tatacttcta tacgtgaagc
    39661 atcccagtgg caaataaaac cattgcaatc actccgacca tcgtcacacc tataagtaaa
    39721 atcttatcta agtggatatg ttgtacaggc tcgttttgct ccttacgtgc tttgaggtaa
    39781 aaataaatgc ctggcaagaa aagaacaaga acaagcatta cttcattaaa ggctacgact
    39841 agcatagccc atacttgaaa ggcacttgct aaaagcccta ctaaaaattg gacccattct
    39901 ttccgctgat aagaaagttt catttggtac aatcctaaga aaagccacgt gaagaaaata
    39961 gctgctgtac caagtgtgta agcaaagtta taagcactgt cagtgaataa taatgaaagt
    40021 aaaaagacat tcgtacataa cgtcgtcaat acgatagcat aagttggtgc cccatttttg
    40081 ttgagttttc cccatgcttt aggcaaaagt ccgtcttcag ccatgtttcc cattgtttca
    40141 atcggtaaga tcgtccatga gagccaaaca ccaactaaag aaatgatcaa accgccgttg
    40201 atcacgactg ctccccattt accaaccatc atttcaaaca catagcccat agcaggctgc
    40261 gccttgatcg tttgcagctg attcatcgtc attgtcccat atggaagcat ggaaaccaag
    40321 acgtaaataa taatcaagcc gaatatccct agaatcgttg ctttcccaac atctgattgt
    40381 ttttttgctc gtgaacctac gacagaggct ccttcaatac caacaaatac ccaaagcatc
    40441 accatgatgc aactcaatac ttgactggaa aacgggatcg gctgtccatt cgcattcaat
    40501 ataatgtcgc tagaaaagtt atcaatgaat tgttggatcg aaaacgtgct gatagctact
    40561 acaataaata aaaataatgg cactaattta cagaatgtga cgatagcatt gacgaaacta
    40621 gcattttcga ctccccgatt cactagccat gcatatcccc ataaaaagac tgtactcatc
    40681 acgatcgatg ccagattttg cccaccttca aatactggga agaaatatcc taatgcgctc
    40741 atcaacaatg ttgcaaaagc tacgttgcct aaccagcatg tgagccagta accccaaccg
    40801 ctgataaaac cagcgaaacg gccaaaacct tcttctgcgt aactaaagat cccgcctttt
    40861 aaatctggac gttttttatt gatattattg atcgacaagg agagcatcaa cattgctgtt
    40921 cctacgagta cccaactgaa aatagctggg ccgccagccg atccggagat gtccgtcata
    40981 ataccaaata cgccacctcc aatcgctgaa ccaattacta aggcagcaag tggaattaaa
    41041 ccaattcctt ttttctgttg ctctgtatcc atgtaagcat cctccaaaat aaaaccgctt
    41101 ttatcaaaaa gtataacaaa ccttttttat ttttacttaa ttaataaaaa ctttaataga
    41161 aatataattc taccggcttt tttctcatta tacaagcaaa gcgcctagtc cctttccaaa
    41221 ttacaccacc aaaaaactta aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat
    41281 ccatgaacat tttatatata tcgatttcag gaaatacgcg tgcctttgcc aaacatctag
    41341 cagaatatgc tgaaaaaatg catgcagaag atcctgtcaa tcctgaagtt actttaaaag
    41401 aaatccatga aaatagtgat tttacgaaag aaacagaacc attttttact ttcgttccta
    41461 cttatttaga cggcggaaat ggtttagata acggtgatac agaaatcttg accgaaacga
    41521 tgcgtgaata tctggaacat gaagacaatc ataaactttg tcttggtgta gtcggtagcg
    41581 gaaataaaaa cttcaataat cagtattgtc taacagccaa acaatatgca caaacatttg
    41641 gctttccttt cttggcagat tatgaattac ggggaacacc atctgatgtg acacgaattt
    41701 atcagatttt agcagaaaac aataaataat aaaaaagaag aagctgaaaa atagatttca
    41761 gtttcttctt tcgtttctat tgtttctatc atattaagct ttgacatcca tcacaggtgc
    41821 ttttgctttt gctttacctg ttttttccaa gacaaagttt tttacacgat ccatattggt
    41881 taccaagacg atcatggatg tttctttgcc tgctgctttg atcgcttcta agtcagcttc
    41941 tgctacaagc gtatcaggtg tcacttgctg cccttctgtt actttcacat caaatggcgt
    42001 accgcctaag tcaactgtgt tgattcccat atgaagcaag atttctaaac catttggcgt
    42061 tgtgatccca atagcgtgtt tcgttgggaa gacagagctg actaccccag taactggaga
    42121 atagatatgt ccgttttctg gttctacaaa gtagccttca cccatcatct tttgtgcaaa
    42181 aactggatca cttgctttat ctaaagaaac aactgttccg tcagcaactg atgagaggat
    42241 agtcacactt tcagctgaag tttgaggtac tactttttca ggcacttcat ttactgtggc
    42301 tgtttcagtc gctaattctt ctttttctaa acggtcttta ggaataccga agaagaatgt
    42361 agcaacgaag ccaccagcat aagctgccaa taatccaagg atgtagccta accatttacc
    42421 gtcagagatc aatgggatca aagcggctcc tgaaggtcca atagcgatag ccccgacatt
    42481 tccgatcatt ccgatcactg ccccgccgat acctccaccg atacaagcag taataaatgg
    42541 gcgtcctaga ggtaatgtta ctccgtaaat caatggttcg ccgattccta ggatcccgac
    42601 tggtaaagct cctttgatca tttctgttaa ttctttatct tttttgcagc gaatccataa
    42661 tgccaatgct gcacccactt gtcctgcacc agccatcgca agaattggaa gcaatagcgt
    42721 acgaccagtt tcatcaatca tttgcacatg gattggagtc aaaatttgat gcagtccgaa
    42781 cataaccatc ggcaagaata atgtgcctag aacgaagccg gcaaatgctc caccaacgcc
    42841 aagtacccag ttgatcccac cgaccatgcc atcagaaatg aaacctgcta aaggcatgat
    42901 gaagaaaatt tcaatcagac cgatggcaat caatgaaaga gttggtgtaa cgatgatatc
    42961 taccgcatca ggtactactt tatgcagttt tttctcaacg attgacaata gccaaacagc
    43021 gaagatgaca cctaggatcc caccttgtcc agctgcaaga gcttcaccag taaataagtt
    43081 tttcagtggt gcttccggat tcatccctgt cagcatcaca acggccccga taactccacc
    43141 aagagttggt gttgcaccaa aaacttgtgc agcattgata cctgtataga taactaggta
    43201 gctaaacatc ccgtttttca agatgttcat cacatcgata tattgttgcc atgttgctgc
    43261 atcaagtgtt ccggctgtga ctaagtttgc taaaactgcg gcaatccctg ccacgatccc
    43321 agtaccgaca aatgcaggaa tcattggcac aaagatatta gaaatgtctt ttaataccga
    43381 tttgatttta gatggttttt gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc
    43441 gtttacacgg tcttttccag atgctgatga atttgctgta gctgtactta cagaagaaag
    43501 tttttctcct aatttcacgc ctgccaaatc aaccatttgc tgtgctactt tgttcacttt
    43561 cccaggtccg atgatgactt gaagctgttc atcttctact acaccaagca ctcctggaat
    43621 cgcttttaat tcagagacat ttacaagatc atcattgatg atcttcatac gcacacgagt
    43681 catgcagtgg acaatgcttt ctacattgcc catgccgcct gcccctttat aaatttgttt
    43741 tgccagttct tccaaagtca gttcgttttt tgccatggtc ttttccccct cataaggtcc
    43801 cttttacaaa tccgtttgct tcagctaatt ttgcttgagc ttcctcttta tcactatttg
    43861 tcaaaatcat gacgatagcc agcttaacat tttgatctgc ttcttcaaag tacttttcag
    43921 ctgtttcgta atcagcttct gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt
    43981 tttcatttgt tggtttgaca tcgaccatca agttattgaa tactttacct ataccaatca
    44041 tactgatagt agaaatcata ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag
    44101 tagagcctgt caaaaattct ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac
    44161 tgatttcggc ttgtttattg caagaaatcg ttcctgtgac agcaccgatc gattttgcgt
    44221 aatctaatcc accgatcaca tatggtgtac gaccgctagc tgcgatacca ataactatat
    44281 cattttttgt caaattcagc ttgattagat cttcttgccc tagctcataa gaatcttcgg
    44341 ccccttcgac agctacagtc attgcttctt ctccaccagc aattagacct atgaccattt
    44401 ccggttctac accgaatgtc ggtacgcatt cagcagcatc taacacacct agtcttccac
    44461 ttgttccggc tcccatataa atcagacgtc cacctttttt aaaggactca atcgtgttag
    44521 caatcactgg ttcgatactt gtcagttctt tttcaaccgc agcggccact ttctgatctt
    44581 ctttgttcat tagttgaacg gcttgagaaa tactcatctc atctaatcca aaagtttctt
    44641 ggtttctgcg ttccgttgtt aggttctcta aattgatttt gctcattttt atcttccttc
    44701 ttttcttata aattgatagt tcataccagc tttgatttgg ttgatcaatg gtagctcttc
    44761 tttgataact ttagctacta cattcacttt ttcatccgct ggcaaatcat atttggttaa
    44821 ttggatctca cccatatagc gcaaatactt ttcgttatct aaagtaatag accctttcat
    44881 acgcgtagct gtatttcttg ctgggatagg cggaatctct cgaaaacgtg catctacgct
    44941 tcgtatgaca tctcttgcat cgtcttgccg attcgtatgc ttccctaata tatattcgta
    45001 aaattcttca tctacaggtt ccgtatggag caagactgtc tgttcttttt ggaaagaagc
    45061 aaattgctcc tgaacgtttt ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt
    45121 acttgctaat agatctagag cgcctgcaag aggatggatc cctcgatgtt cttccaaagt
    45181 tggcaatcct tgatatagtg ggcctctcaa ctctgtatct cctggtacaa aaccttggac
    45241 aaggagacct tgaattttga gccattgatt ttttttctga taccattctt tgtccagccc
    45301 tgtttcggga cgtggataat aattatgcca agcttcaatc tgtgagaaat cagcttctgc
    45361 ttctttcaat tcatcgatat ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa
    45421 ttgatgtgac cagtcggcaa tctgctgatt ggaaatatga taatccattc ttaaacctgt
    45481 tacaccaatt gctttcaaag gcgttagatc ttctaatgaa aaacccgctc gttccaatgc
    45541 ctctcctgaa atatcgacca tcaattctag tttattcttt ttcgcttcgc ttcctagttc
    45601 gatcaatcgc ttttgatatg ccgaaacatc atcttctggt atatgcatgg aagtgaaaat
    45661 accgacaaag ccattggatg ccatttggcg gatatactgt ttcttctctt ctgacaaaga
    45721 atcgttcata aatattgaaa agccaaacat attatccctc tttttctgta tccgtcttca
    45781 tttctatgac tagagtatat atctaaagaa acaaaatttc aatactttta ttaaaataat
    45841 taaatttcat ttctaaaggt tgagtttata ttatcaaaag aattgtcata acctaaaaaa
    45901 ggcactgcta agcaaaagca gtgccttgaa atattttatt taaataacac aaaaacataa
    45961 ttactcataa gaaaccaatt ctgtattatc taccatgaat gtaaagaacc cctcattagg
    46021 catcggatga aaataagcat tgatcactct tatcaaggca ttatggcaaa ctaacagata
    46081 gacattttct tcgtcttcga tgcattcttt caataaagga acgatccgtg catacacatc
    46141 taagactgac tccccatttg ggaatcgtac agcgaacgct aaacgtgctt tttgaaagtt
    46201 ctcatctttg cttggcagtc catcataatc cccaaaatcc atttctacca atcgttcatc
    46261 aacggtcagc ggcaagctga gacgatcagc aactgcttgt gctgtatctc ttgctcgttg
    46321 aagaggggaa tggatgattt tcgtgattgg aacaggcaaa tctactacct tttctgctag
    46381 tttctgtgcc tgcatctgtc ctttttcagt cagcggcaca tctgcccgtc cgcaaactaa
    46441 tccttgtgcg ttccaagttg tttctccgtg tctcgttaca taaagcatgt gattctctcc
    46501 ttcgttggca aagttcccct taaccatacc ttgttgttct tgagattaca agggttagat
    46561 tctctcaaga gaaataatct cagtagatac tttatccagg aaggtttgat catgttccac
    46621 tagtaacatg gcaggtttca cgttcaagat cagttgttct aattgttctt gattgaagac
    46681 atccaaataa ttcaatggtt catcccatgt atatagttca gctggctgtg ataaagattt
    46741 agccaattcc acttttttcc gttggcccat actcatctgc tcgatcttgt tatgaaaaac
    46801 atctctttcc atcccaagct ttcggaggtt gttcaaaaat gcttggtagt ctacttggtt
    46861 tttctctgca aattccgcca acgttcctcg attgtcttca taattttggc ttgcataact
    46921 aatgctcaga tgttttggct ggtatttttc tcctataacc ttgccgttga atgcccccag
    46981 aagataatgg ataatggatg actttcctgc accgttagga cctgaaatcg ccacccgctg
    47041 atgaggctcg attgtaaaat gaattggctc gaataacaga ttttcatacc ctaattgaag
    47101 atcttctacg cttaaaagtc gcttatggtg agacgcttgg ctattcatcg tcaaagaatc
    47161 gatatattcg atatctttta atagtttttc tttctcgctg atctgggtct ccatccgatt
    47221 cacgattgct ttggatcgtt tcatcgtccg tgcagcatca gcacccactg ctcctttatt
    47281 cactcgtcta gattcagtat cgatgaatcc gacttgtttc tttgttttat ctccttctcg
    47341 ggaacgagac cattcggctt tttcagctgc tgttttcttt agcctactga cttctttttt
    47401 caatttttca ttttgagcca tttcaaattc atcacgaagt tttttctgtt cttcatagat
    47461 agagaaattc ccttgataaa gttccagttg acttttttcg attgctaaaa catggtccac
    47521 tacttcatcg ataaatcccc ggtcatggct gaccacgatg aagccttgtt ttttcttttt
    47581 caaataagcc gctacttgtt ttctaccaga gatatccaaa tgattcgttg gttcatcgat
    47641 taacgggaaa tgagtgtcat ccacaaataa aagtgccagt aggacttttg tcttctctcc
    47701 ccccgatagt gtgctgaatt ctctccataa gatttcagga tctgtttgca tcaattggag
    47761 ctctctttcg atttcccata tctcaaaatc cgtaatatca tttaacacgt aataggttaa
    47821 acgttcttta tcttttgtct gttgcgggaa ataggcaaat tcttgctgat ggattacttg
    47881 cccttgataa ggtagtttgt tttgtagaat attcagtaaa gttgtctttc ctcgaccgtt
    47941 tcgtccgata agtcctagtt tccattgtgt gtcaaaattt agatttgctt gttcaaataa
    48001 taatgtgcct tggctgtcgt agccgaatgt cagatttttt atttcgattt tagacatttt
    48061 ccatcacctc gtaaaaaaag accagtccta ttttcagaac tggctaaaga ggatgcaagg
    48121 gttggataca cacaaaaaga cccatacatc gaaaaaacca agccaatccc gaaaatctgc
    48181 acactgcttc cttgtctcca caaggtacag tctgaatgca gctttcatca atcgacttaa
    48241 tttttcaatg cacggatccc tcactttgtc ctatatttga aattacgata gcataaagaa
    48301 aatgattttg tcaactactt cgggaggatg gtgaaaaatc tgctaagtgt ctgtcctaag
    48361 atttttgttt tttctaatgt ttgcctgtgt tccattgctt cttctaaacc tattggaccc
    48421 aaatgaatac ttaagacaat tgggagaaaa tcagaaagtc cttctagctc ttggtctcta
    48481 cttccgcaaa tagcgattat tggttttcga tatttttttg caagcactgc tacaccgtat
    48541 ggaacttttc cgtggatcgt ttgctgatcg atccgccctt ctcctgtaat gatgacatct
    48601 gcttgttcga tctttttttc caatccagtg atttggctga ctaaatcgaa accagaaacc
    48661 atcttcgctc ccaacaaaaa aagggctcca ccaataccgc ctgctgctcc acttcctgga
    48721 atatcatcta aatccacctc ccagaaatct tttatttgct gagcaatctt tcctgcttgt
    48781 ttatctaaga aagctagcgt cgtatctatt cctccttttt gtcttccaaa ttgccaagca
    48841 gctccttgtt gacctgtata tggattggta acatctgcag cgataatcaa ttttacttta
    48901 gaaacaagaa cgtttcttcg atcaaaatga agttttttag tagataataa aggatttcct
    48961 tctgtgtacc cttctaaaga agcacccaag gattggagta atcctagacc accatcgcta
    49021 cacccgcttc ctcctaaagt gacaataatt tgttctactt cttgaagcag tgcatcttta
    49081 ataagttctc ctaaggcata actggaagca agtcggatag tttgatccgt tggtttatca
    49141 agtagatcta accccaatgc ttgtgcagat tcgatgactg cggtgttttt tccttgccaa
    49201 gttgtcaata aatagctgat ttttcgctga cggaataaga gatccatccc atcaatggta
    49261 cgccatgttc ccctgagcgc gtaatagatt gcttctgcag tattttctcc gccatctgca
    49321 atcggcacat tgatttttct tcctttacga tcttcccagc catttagaac tgcctgattt
    49381 agttcaaatg aagaagcact tcctttaaat gaatcaattg ccgccaaaat atccatctgt
    49441 atccctcact ttcttcttaa gtaagacact ttttttcatt tttttcaaac aaaacaatgc
    49501 tattttcttc catttttcta tatactaaaa gggaactcat tgaataaagg agagaaaaaa
    49561 catgaaaatc gcaatagctg gggccggagc aatgggcagc cgaatcggat tgatgctaca
    49621 tcaaagcgga aatgaggtcc tcttgatcga tcgttggcct gctcacattg aagccattcg
    49681 tacaaatggg ttgattgctg actttaacgg taaagaagtg gtcgcaaaac tgccaatcta
    49741 ttcaccagaa gaaatcatcg aaagcaacga acacgtggac ttgatcgtag cattaacaaa
    49801 agccaatcaa ttagatgata tgttttgttc gatccaatcc attattactg acaatactta
    49861 tgttttgtgt ttactaaatg gattaggtca tgaagatgta ctggaaaaat atgtgccaaa
    49921 gaaaaatatc ttatttggga tcacgatgtg gacagctggt ctagctggtc ccggaaaagt
    49981 cacattgtta ggtgatggag aaatcgaact agagaacttg gaaccagaag gcgaagcatt
    50041 tacaaaaaaa gttgtagaag tctttcaaga agcaaattta aatccgatct atagccataa
    50101 cgttcgttat tccatttggc gaaaagcttg tgtaaatgga acattgaatg gtttatgcac
    50161 gattttggat tgtaatattg ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg
    50221 aacaatcgta tctgaatttg cttctatcgc agcaaaagaa ggaatcatac tagatcaaga
    50281 agaagtgtac caacatattg cttctactta tgatcctgac aacatcgggt tgcattaccc
    50341 atctatgtac caagacctga tcaaaaacca tcgtttgact gaaatcgact acatcaatgg
    50401 tgccatttgg cgaaaaggac aaaaatatga tattgctaca ccatattgtg cctttttgac
    50461 ccaattagtc catgcaaaag aaggaatcct tggcgcagaa taaaaggctc tctgatgaga
    50521 atcagctgaa tcagcagctg gttctctttt tttaagccca ataaaagatc aaaaagaaaa
    50581 cacaactctc atgatgccat aagaattgtg cttgtttgtt tcagtacata atctcttcaa
    50641 aatgtaggtt ttttatttgt tttcaacaga ggagaggtca tattcgtatt gctcataaat
    50701 agtggaatcg ttagtgataa atcgttttgc ccaattttct gcatcataaa attccaatag
    50761 ctctgatagg attggcttaa atggtgtatg cagaaattcc ggtaattcat ctaaatcacg
    50821 ttctcgaagc aaaaaaatat tttcaggacg ataaaaatga taatgagcaa tcgcttgatt
    50881 atccgtcact agtttctttt gaaaatacat ggattcaaat gtccgcaaag tcactccttg
    50941 ctgaccctcc cgcaacagtt ccagaatccc gcgcgattcc cctgtttttt tcaggtactc
    51001 ttcataagta agatatccag gttgttcatt ccctcgatgt ggaagaatga aatagttggt
    51061 cgtaacatct ctttttataa attcctgctt atagctttct gctcgttctt ttctcccatt
    51121 attcgttgca ccaaaaaata gatccgtctt tggttcgcta gaagaaaaat tcatatgttt
    51181 cgaataaaat gaactattat gtttcagccc aagttctttt gcttccaaaa aatcaaaatg
    51241 ataaatctca tcgattgccg gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc
    51301 tttgaagtga ttccaaaaaa ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg
    51361 agccatctcc atatcaaatc cataatctaa aaaaacgatc agctgataat ttgccaactg
    51421 ttgtctagct gatttaatga attccgactg tagtaaatct tttccggacc aaacatctaa
    51481 agagcggatg gaaggcaaaa aataaagtga tttatttttt gcaactagca gtacgtcatt
    51541 tttgttcatc ttttccgcct cactttctct gtcacttttt cctatgatag cctgtttaaa
    51601 tagtaaaaag ccagtaaaac gtttcctata tttgtatgaa aatttaacat ttgtatgaaa
    51661 atatgatgaa ataaatcatt cttctattaa aattcgccta aaagttttct gaatagttgt
    51721 aattgtctga aaatttgtta tcattagtca ttgttaaata ttgctattta ttcttaatat
    51781 aaattttgat tgtgaattta aaagaaagag gtcatttatg gaagataaaa aattgaaaaa
    51841 acaacttagc tccagacata tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct
    51901 atttaaagga agtggcgaag cgattgggat cgccggccct tctgtattga ttgctttttt
    51961 gattggtggg ctgattttat atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg
    52021 tggcgacacg catcacggat tatctggttt gatacgccct tatctaggtg ctcattctgc
    52081 tgattttact gattgggtct actggtctat gtggattatc aatatcatcg ctgaagctgt
    52141 tgcagctgct tcctttttgc agttgtggtt cccgcacatt ccagcctggg tatttgtttt
    52201 tatgttggct gttttaacta cgatcatcaa tctttattcg gtacggttat tcgcagaaac
    52261 agaatattgg ctggcttttg ctaaaatcag tgtcattatc ttactgatca tttttgctac
    52321 ttatcttgtc ggtcagcaaa tgttgggcac aggcgttttc cctacccttc agcaattgac
    52381 tgaccatggt gggtttactc ctcatggtat gaagggaatc atcagttctt tgcttgtagt
    52441 tatctattct tacggcggtt ctgaactgat tgctatcacg gtaagcgaag cagatgatcc
    52501 taaacgtgcg attcctaaag caatcaaagg tgtcatggga agaatcattt ccttttacat
    52561 cattcctttg tttttgcttt tgattctttt tccttggaat actttagcag gcacgaatgt
    52621 cagtcctttt gtgatggtat ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa
    52681 ttttgtgatt gtccttgctt tgttctcatc tatcaattcg ggtgtgtatg catcttcaag
    52741 aattttgttc ttccgtttga aggatcgaaa aggttcctct agaaaactag ctgttttaaa
    52801 caagcatcaa gtaccgcaac gtgctgtttt attttgtacc agtgttcttt atcttggtgt
    52861 agctctttct tattttgtcg gtgacaaatt atttggctat ctggcaggtt ccctttcgta
    52921 tacagtatta ttgatctgga tcattatcag tgcggcatcc tttgtactgg cactaagaaa
    52981 aggaacatta tgggataaag gaatcagctt ttttgcatta gctgtcttag gattgatttt
    53041 ccttggtatc ctgttcacga actccattgg ggtaactgta ttgactggcc tgctttacct
    53101 gttcatttat ttcagttatc aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa
    53161 tcgatagaaa attttatttt atttatgtgg aatagatgga gctattgaag aaaggagttg
    53221 tgacaaaagt cttgttgcaa ctcctttttc cgaataaaca gtctcatgac ctcttggtaa
    53281 acggtgttca aaaaccctct gtgcaaaata attgattgtt ctccatgctc atgacatgat
    53341 agaagaaagg aatgaaaaaa aggagtttgt tttaaatgaa aaagtattgg aagtttttgt
    53401 tgatgatcgg tgtttcaacg gtagttatgt ttggtatgat gtacttgaat gtctatgcgc
    53461 tcgatcatat cttcttcagt cgtacacgag tattcatggc gttgatgatg ggtgcagtca
    53521 tggcagtcat catgctgttg ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta
    53581 tgatcttagc cgctagtctt cttgtttttg ccggttcctt gtttatggta cgaagccaaa
    53641 ctactgtcaa cgacacagct tggatgaagg ccatgattcc tcatcattct atcgctatcc
    53701 tggcaagtaa acgagctgat ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca
    53761 ttcaggcaca agaagaagag atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac
    53821 cattatccgt ccaaataaaa aaagaggatg ttccagaagt tatcagcttc aagaaataag
    53881 aagaagctac ttctggaacg ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag
    53941 taataagaac agccaaacaa aaatagcttc tcctattttt cgtttggtta ggcttattac
    54001 cgcagatgca agcttttgaa caccgtttat taagaggttg taaaatcagc gttctgacct
    54061 gagtattaga gagacaaatg gaaaaatagt tcctcatatt ttgccatttt ttgagctaat
    54121 accgagggtc agctgattga acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt
    54181 cgcgtattaa gaaaaatttt gtgaaatggc tttccacatt tttgtataaa tttggcttaa
    54241 taccgagaaa ctggcttttg aataccgttt attcgtaaaa gggattgtga caaaagtctt
    54301 gtcacaatcc ctttttatgt ttttattgtt ctaattgcgc tgtcttgctt actgcttctt
    54361 gcggtttcac aaattttttt gagattcccc aacctgtgaa ggcactgacc atcgaaaaca
    54421 caggagaaag caaactgaaa aatacgaatg gcaagtaagt gagtgtagga ataccaaatg
    54481 tacttgctac gaaacttccg gcaattcccc aaggcactaa gtagttgatt actgttccac
    54541 catcttctag cgttctacct aatacagttg gatcaaatcc tgctttttca taagtttctt
    54601 taaatgcatt accaggcaga ataacagata ggaattgttc accaataaaa atatttactc
    54661 cgatacttgt catcaatgtt gtaaaaatca aatttggtgt gttcgtcatc tttcttgata
    54721 ctcgttccat cacggttgag atcaagccaa attcgatcaa caatccacct aatgacaaag
    54781 ttagaataat caatgacaca gtaggcatca tactttcgat tcctccgcgg ctaagcagtg
    54841 aatcgatttg ttggtttcca gtatttgaaa caaaccctga ttcgatgacc ccagccaact
    54901 tcgttaagga agtttgtggg ttttgaataa aaatcattcc tactgctaca gcgatattca
    54961 acaaaatcgt taaaattgcc ggcacctttt tccatgcaca cagtaacatt aaaaataaag
    55021 gaatcagtga ccagattgaa atagtaaagt ttgcttctag aacatcagtc acttcacgaa
    55081 tagatgaaag acttgcggct ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa
    55141 caacaaatgc cgggacagtt gaccacatca tatgcttgat gtgtttgaac aaatcggcat
    55201 caacaatagc tgctgctaga tttgttgatt cggataatgg agacatctta tctccaaaaa
    55261 cggcacctga aacaatagct ccaacaacca gcgctggatt taagcctagt gttgtcccaa
    55321 taccgaaaaa tgcaatccca atcgttgaca taacagtaaa ggcactccct acagcgctac
    55381 ctacgattgc acacacaaca aatacagaag ggacaaacca ctggacactg atcaatttga
    55441 acccaatgac cattagagtt ggaatgatcc ctgcttgtat ccatacagca atcaatgaac
    55501 cgactaaaat aaaaataaag attgggatga ttcctggttt gatgccattg atgattcctt
    55561 cgtgcagcca atctgtcggg tatcttttta tcactgcata caacatgacc attccgatcg
    55621 ctgctaagat cgtgatatta ggtgagacct ttaatccgac aacgccaatc gcaatgccta
    55681 aaacaataac cattaataca agtgttgctt ctaaaaaact tggtgttcct ttttttccca
    55741 tgataatctc tcctttttta gttgttctta tgttctaaaa aagttaagat atcggattac
    55801 tccgccgcaa ttcatccgcg attcctcctt tataaaaata aaaagacctg ttttaaacta
    55861 aggacgaatg actccgcggt accaccttaa ttaaaaacag atctgttttt cactttcaaa
    55921 cttttattca aatgtatgga ctttccatgt aattcgtcaa ttaggcttga tcggttcaca
    55981 gcgaccaccg actttctgaa caaaacacct aaccgctact gcgtgaaaag ttgacttgta
    56041 cgaagtttac cttccataag atcatttgtc aatgaaaaag aaaatatttt ttcatttttc
    56101 tgaaaattat ttttataact ttcacacaca agtttatttt ttactagaaa gtagcgccct
    56161 cttgtttttt tcttgctact atttcttgat gttcttgctt gattctttct aatagctttg
    56221 gctctttcaa cagatcaaga gctgtattgg cgagtagttc tgctgcaatc gcaatagaag
    56281 caaggccttt gtcactttta gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg
    56341 tctcagagat tgctaccgta ggctggattg ttggtatgac ttggctgaca tttcctacat
    56401 cagaagaacc gagacttgtt ctttgttccg tttctatttc ttcatctgga acacctgctt
    56461 cttttgcatg ctggaagaat aagtcatcaa aagaaggagt aacgatgaca tcatcaactg
    56521 cattttggaa caatccaaac tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga
    56581 tattttccac tttttcatat actttatcta atgtttgccg atttcctgca cgtaaataga
    56641 aacgtccagc agcgtaatct ggaatgacgt tggcagctac tcctccgtct gtaatgatcc
    56701 catggatatt gacatctttt ggcaattgca agcgtagagc gttgattcca ttaaacactt
    56761 ggatcaatgc ttccaaagca tttactcctt tttcaggtgc tgctgctgca tgggaagcga
    56821 caccgtaaaa tttgatgtcg actggatcat tcgctaaaga ttctgtcgtt ttcccataac
    56881 gaaaagctgg atggacgcat aacgctacat cgacatcctc aaaaaaacct tctcgaacaa
    56941 agctgccttt agctgatccg ttctcgcccc cttcttcccc tggggtgcca tatacacgga
    57001 tttctccacc agtttcatca atgaattgct tcactatgct ggcggctaat acggagtaag
    57061 taccaaacaa attatgcccg catgcatgac caatacctgg taaagcatcg aattctgcca
    57121 aaaaggcaat tgtgggaccg ggtttattgg aaatataacg tgcatcaaag gctgttcggt
    57181 gtccagctac ttctttttct acccaaaacc tttctttttc taattgttta atcaacgctg
    57241 ctgaggaata aaactcatag ttacttactt caggatgttc gtggatatct agagcaagtg
    57301 cttgatattc tggtaaacga tattggatta gatccttcaa ttggtttgtt gagattgttg
    57361 ttgtcataaa aaattcctcc tatgctagtc atttttctta ttttctcccg aatgtttccc
    57421 atgcaggcac gcttgatcct tttgatgttt tctcgattac tttgactgtg tcttctgttt
    57481 gataagcgtc tactattttt tgatacgttt tattgttttc atcttctttt cttgcaacga
    57541 tgatattcac ataaggacgt gcatggtcat ccactggttc taagaagatg gcatcagaag
    57601 caggattaaa tccagcatct acagctacac cgctattgat cactgaaatg tctacatctg
    57661 acaaagcacg tgctgtttgg gaagcatcta attcagaaat gtctagattt tttctattct
    57721 cggtaatatc agaaacagtg ggcgcttgtc cttttgcctt atccactttg atcaatccag
    57781 cagtttgcaa taacagaaga gctctgccgc catttgtaac atcattaggg attgctactt
    57841 ttgctccgtc tttgatatca gctacatcct tcaaagtgtt agaataaatt cccagcggtg
    57901 cattgacagt atttccgatc gatactaaat cagttccgtg ttctttattg taattgtcta
    57961 aaaagaattg atgttggaaa gaattcaaat caatcgatcc atctgctaaa gccacatttg
    58021 gttgattgta atctgtaaat ttcacgtatt ccaaattaat gttctccttt ttcaaacggt
    58081 ctttcacgtt gtcccataca tctgtatctt ctccaataat ccctagtttt acgatagttt
    58141 cttttttgtc agttgtatct gcttttcctt ggctacaccc tgccaaccaa ataattgcta
    58201 caaatgtaat gactacaaaa cgaataattt ttttcatttt ttctcctcca ctttcaatgt
    58261 gtaatttttt taatcagttg attactgata aattgactta aaaaaactaa gattagaatc
    58321 aataatgtag ccacaatcgt tacatctgtt tggaatcgat tataccctct cgatatagca
    58381 agattcccta atccgcctgc acctactgct ccagccatgg tagtcagtcc gatcacgttg
    58441 atgatagtca acgccgagac ccgaatgata ccaggcaaac cttctaacaa atacacacgt
    58501 aaaatgatac cgattggact ggttcccatc gcttctgccg cttcgatcac cccaggatca
    58561 acttctagca gtgcgttctc gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat
    58621 ggcactaaag ctgctttcgc accaatcgtc gttccaacta agaaacgagt agtcaatgct
    58681 aaaagagaaa gcaagataat gaaagggatg gaacgaatga tattgatcag cttatcaagt
    58741 atttcaaata gcagacggtt agctagtaat ccattagggc gtgtcactac taacgtcaca
    58801 cctaagatga ttccaatcat cccgccaatg aatgctgtcc aaaaagacat atatagtgtt
    58861 tgcttcgtcg cttcgacaaa ttcatccgga atcaaataaa cattgggcaa atatgtttta
    58921 aagaaatcag tcattttatc ttcctccttc gatcactttg atacgactat ctaaatcgtt
    58981 tttttgtaaa atatttattt ttacaccaga tttgatcaaa taatccaatg cttttttccg
    59041 ctgtttggtt tctccagcta aagtaacgat caaactccct aacggcgttt cttgtaataa
    59101 ctctacattg ccatataaaa tattcgctgt tacttgatat ttgctgtaca gatgagcgat
    59161 tagaggttcg ttcgtctgat ttccaatata acttaattcc accagccatt tattggcgat
    59221 ctgatcggca aaagcagaat gggcaatgat cgtttctaat gcttggtcaa gatgagtagc
    59281 tgttcgaata aaatctttgg tcaattcttc ttcaggattg ctgaagatct ggatgctgct
    59341 acctttttca acgattcttc catcttccat aaccgctacc ttgttgcaga tttctttgac
    59401 aacttgcatc tcatgagtga tcaggacgat tgttaaacct aactggcgat tcaatttttt
    59461 taacaatgca aggatctgaa gggtcgtttt cggatcaagt gcactggttg cttcatcaca
    59521 taataatacc ttaggttcat tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc
    59581 ccccgacagt tgtttaggat aagcagatgc tttttcttcc aaaccgacaa gttccagtag
    59641 ttcattcact ttttgacgac gttcctgttt cgattttcca gaatacttta aagaaaaatc
    59701 aacgttgtca aaaatagttc ggctttccat tagattgaaa tgctgaaaga tcataccgat
    59761 ggcctttctt tcttttcgca attctttggg agagagctga ctcagattcg tctgattgac
    59821 aatgacttct cctgctgttg gtttctgcag caaattcatc acacgaacta acgtactttt
    59881 ccctgcacct gagtaaccga cgattccata aacatcgcct ttttcaatcg ttaaatctac
    59941 agaatctact gcctgtatct ctttttcttt ttgtttgaaa catacagaaa catctcgcaa
    60001 ttcaatcatt aaaattcctc cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca
    60061 ttttcttgag gacgcttttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca
    60121 actcctcttc aagtacgatc atactctagc aagatatcgg tcgcagccgt catcgcctta
    60181 catctatgct cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt
    60241 ctcagctacc gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa
    60301 taatattcaa aaaaaaagtc cttttactaa cataaactat gtcagtaaaa ggacgacgta
    60361 tcgcggtacc accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga
    60421 gattggcgtt cgttttaacg gtcgaacagt tccgaatttg cctacttatc gacaaattat
    60481 tctccaaggc cattttcagc ttttcttact tgactcttct caccagccag agcctctctg
    60541 caaagtttcc aaacttactt tccttttcaa cgaaatcgat attttgttgt tgggattaat
    60601 ctacatgagg aaaactgaaa agtcaagtac gaaattcaaa ttattttttc tatatttatt
    60661 tttctgtttt tttatttaac atttcaataa cagttcgaag atcttttcct tccgcaagaa
    60721 gtttcaattt ttccttttct tcgacggtat aatttcggac aaaactggaa tcaggtatca
    60781 aaacaacgcc taatcctgct cgtgaagcag ctgttgctcc tactaaagaa tcttccactg
    60841 caaaggctga actagcttct aatcctgttc gttccaaagc tgtcagataa ggaatcggtg
    60901 aaggcttgtt ctccgacact tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt
    60961 taaagtgatc caagatatct gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt
    61021 tcatatgacg ttcttttaag tctgccaata tttcttttgc atatggtttt aagtggatgg
    61081 ctccgcttgt cagctgttga taaaaatatt cttcacgctt cgctcggact tcctgtactt
    61141 tttcagggct acctgctgct ttcactaaaa tatcaaaact ttgtttccag ttttttccgc
    61201 cccaagcgtc aacgatttct tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc
    61261 agccatcacg atacgttttt tctgtgtcaa ctaacaaacc atctaaatca aatattgcac
    61321 ctttcatcta tacattaatc ctttccaaaa caatacacat agtttagcac gactggtcca
    61381 aaaagaacag aagcaaagaa ccagccttct cgtttataag agttcttgga atcgacggat
    61441 atagattttc ttcacagctg tcacaagcgc cagataagcg atgactgtag caaataacca
    61501 taaccagtaa ttgccaggaa gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg
    61561 aaggacagaa ccgacaataa ttcctaatgt agtgataaca aacatggcaa atgttgcgtt
    61621 gctttggata aatggtactt tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca
    61681 tagtgattcg acaaaccatc ctgcatggaa taatgcgatg aaaatcactc gctgtgaact
    61741 atccaatgta tggaaacttc cgcccactac agcaggacaa ataacaaaat acatcaacaa
    61801 gtaagtagta atatcaaata tcgaacttgt tggaccaaag taaaccatga attccccaat
    61861 actagatgca ttccattttt ttggctcgtc caaatattct ttatccatcc gatcccatgg
    61921 caaggaaatg caggaaacat catagatcag attcaaaaac aaaagttgta aaggcagcat
    61981 tggtaaaaat ggcaggaacg tacttgcaat cagcacggaa aacatattgc caaaattaga
    62041 acttgctgta gccttgatat atttcatgat attcccaaaa gtttctcgtc cagaaagaag
    62101 gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgcag attctttcgc
    62161 aatatccaca gctgtatcta cagaaatacc gatatcagcc actttcattg caggagcgtc
    62221 attgatacca tctcctaaaa atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa
    62281 acgtgctttt tgctgaggat tcaattttac gaatacttca tggtcttcag ctacttgtgc
    62341 taattcatga tcactcattt ccgagatttt ctcaccggta accaattcat tgatctctag
    62401 acctacttga cggcacactg aacgtgtaac taattcgttg tccccagtca gaacttttac
    62461 agctacacca tgttctttca gagctttcaa cgcttgtttc gttgtttctt ttggtggatc
    62521 aaggaaagca agataaccaa tcaaaaccat gtctgactca tctttgactg aaaattctcc
    62581 aacgacgctt ggattggtct tttgtgcgac accaatcact cgtaagccat cttcattaag
    62641 tgactgtact tttgcaagta cggcttcttt tcgttctttt gttaatggtg taacagtacc
    62701 gccaagatca ataaatttag aaatgttcag catttcttca atcgcgcctt ttgtgatcat
    62761 ttgtgtttta ccagctggat cagcaaccac aacactcata cgacgacgtt caaaatcaaa
    62821 aggaatttca tccacttttt gataattgat cttatctgta tctaaagttt ctgttgcttc
    62881 atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc tatttagata
    62941 cgcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat
    63001 gattttatct tgtgtcaatg tccccgtttt atccgtacat aacacatcga ttgcaccaaa
    63061 gttctggatg gcattcaaat ttttgatcac tgttcctttt ttggccatca cacttgctcc
    63121 tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggtgttagac caactgctac
    63181 agatagtcca aaaagaaaag cttctagcca atcccctttt gtaagcccat tgatgacgat
    63241 aactgctgga gccatcaatg acataaattt gatcagtagc atggatgttt tatggatgcc
    63301 taattcaaag cttgattgaa ttggtttttc agatagcgtt tctgctacat gaccaaatag
    63361 tgtttcattt ccaacagaaa caatgattcc ttccgcactt ccactgatga cattacttcc
    63421 cataaagaca agattttcgt aacttgtttc gctttgctga tcgtcttcac atactttcgc
    63481 atgcttttct actggatagc tttcccctgt caatgctgct tgggaaatga ataaatcctt
    63541 tgcggacaat aaacgcatat ccgctggtat catatcccct gctgaaagcc gaacaagatc
    63601 tccacagacg atttcttcca taggaatctc tgtgtacttt ccattacgtt ttacagctgc
    63661 cgttactttc acaagtgatt tcagtttttc agccgcttga ttggaacgta cggactgaat
    63721 caacgtcatt gtcccgctga cgataaccat agtgaagata atggctacac cgaaaagatc
    63781 tttatctcca ggagctgcta atacatattc cgtgatgaat gaaataattc ctaatgcgat
    63841 caacactaat gtaaatggcg tgatgtatgc tttcaatatt tctacaatga atggtgtttt
    63901 tttgccatat gaaatcgcat tttcaccgta attttctctt gaacgttcga tttgttcttc
    63961 tgtcaatcca tttgctgatg catggaatag ttcaaataat tcttgtttcg tttttccagc
    64021 aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcaatct ctcctttgtg
    64081 tccaaaagac agctgttctc gctgcttttg gtctttagct attcatcgga tcgatagaga
    64141 aaaaactgaa aggggctgag aacagataga taatagccct ggcctttcct ctagatcctt
    64201 gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca
    64261 tgacttccaa tcatttttcg aatagttatt tttccaatta gaccacctcc tttttatttt
    64321 tcaccgatgg ggatttgcgg caacaaaaaa acctctcgcc aaatactcag cgaaaggttt
    64381 gaaaaagcat attaaaaaaa gcttctcaga tctcatcgtt gagttttgac actgtacaac
    64441 gtaaaaagca gatacctgct tttagctacc ttatgaaaag ccttatctcg acaattcctg
    64501 ttctacccat tggcgtctct cgacgttttt gggcagtagc ctatgtttgt ataggagcct
    64561 cacctaacaa atcttattcg gttttccttc gttaatataa caactaaaaa agaggatgtc
    64621 aaatcaattt tccatgtttt tcatactttt tttaaaatac acacgttata ttcctgtctc
    64681 acgcggtttt atttgtctca ttttccacct cccgctataa tagaaatata gtaggaaacg
    64741 aggtgctgtt atggaaagaa cacttgttat tatcaagcca gacggcgtga gacgccattt
    64801 agtcggaagc atcatccaac gttttgaagc aaagggactg gccattgctg aaatgaagtt
    64861 tgaaactatg actcctgaat tagcaaaaga acattatcag cacttgactg agcgcagttt
    64921 ttttgatgaa ctgatcgatt acatgacttc cggtcctgtc gtttatcttg tgctggtcgg
    64981 cgaggaagtg atcgatatcg tccgtaagat ggttggagca acaaaagctg ccgatgctgt
    65041 tccaggtacg atccgaggag actacgcgct gccggggact gaaaacatca ttcacgcttc
    65101 tgattcccgt gatgctgcgg taaaagagat cgcccgcttt ttcccgccat tcgatataaa
    65161 caaaccgtct gaaaaagcgg cgaagtgata caaattctaa actgctggtc tagttgctgg
    65221 gttcagcaat ggccagcagt tttttctcta aggacctccg ttgattcttt cctaacatat
    65281 attcgagcac tgtgaagaat tcttggtagt ctttcgtctt ttcttcccct aaagcagcag
    65341 aaatcaatct ttgataataa tctgccatca atgtttcata gatcactgta cttctttctt
    65401 tatgtgctaa gctccgtttc atatcttcat tcacactacg tgcctttttc aaccgaccat
    65461 tttctatata aaacgaagtc aggttcatta aaaaagtaaa gagatactgc tcatgattgt
    65521 tcaactgtgc taatcgtcgc attctttttt tgattttcaa ataattgttc tcaatgaacc
    65581 gatcactgaa tatccaagga aaatcagcta gaatagatag ttcaaattga ccatagtctg
    65641 gtatctgcat caagtattct tcaatgattc ggcattcata acgtaaaaat tcaaattctg
    65701 tctcagatac ttctgttttt tcgaccatgt gaagattcaa aaccaaataa tacagatctt
    65761 ctgttttctt aaaatgccta cggttattgc tgatctcttt ataaggatca aagctgtctt
    65821 cttgtacccg atctagcatt cgtctttttt tctttaactt tattggttca tccagctggt
    65881 ttcgcatcca ttcatattct gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg
    65941 ttacaaatga catttttttc actgaatttg tctcaatccc gactagagaa gtcttaaaac
    66001 agattccttc cgctaattgg gactgtgtca acttcttgtt gactcgaagt ttcttgatca
    66061 attcaccatc atacataata gttctccttt ttaatttaaa tttttccaca ttcaataaat
    66121 agatacgtta aaaaggaaaa atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa
    66181 taccaacctt caagcattag aatgttgtct aactactgaa ggttggtttg attgtttatt
    66241 gattattttt ttgctgttca acgtctgttc ttgttactgg atataagttg gttgttgttt
    66301 tattaccatt ttttgaaaag acacttgtcg aatgttcgcc tttttgcttt tcgattgctt
    66361 cattacgttc cgcttcattt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt
    66421 ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag
    66481 tcttgacttt ttcctgatct ttggagattt cctcatcgat tcctgctgct ttcgtatcga
    66541 tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca
    66601 caaagttccc atttcgaaaa gcaactactt gatcatgctg agaagataat aagtctgtac
    66661 caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat
    66721 cgatttctcc accgtattca tgattgactg tccctttagt ataaccaggc atatggatca
    66781 tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac
    66841 caagagcgct tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa
    66901 tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca
    66961 atgattgatc cagataatgt gccgttcgaa cataattatc tactgtgcta ttgccagtat
    67021 tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg
    67081 aaagaaattt cgtgtaaaac ggctgttgta gatgctcaag atattgggca gattctctga
    67141 ataaatcttt atctaatatc ccataaccta acgtttcgtc ggattcatca aaataagatc
    67201 gatcaaagaa attgtcataa cctaagtttt tatatacatg atctctattc caaaaactag
    67261 ctacattccc atggaaaaca gcgcttgtat agccttgctt ttgtccaagg atcgctggtg
    67321 cggcttgaaa gacattgtct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa
    67381 atgtgccagt ttccaacata ttttcagcat cacttgtctt tccctgtcct acttcatgaa
    67441 agaaattgtc aaagctgatc gttgcttgat tctggaaaat actatttaaa aacggtgtga
    67501 cttcttgtcc gtctaccttc atattaatca aaaactgctg gaaactttct aaatggagaa
    67561 cgatcacatt tttcccttta gcgatcccaa atgtttcggg atttggctct gcataatgtt
    67621 tctttgtata atctaaaact tcatcaatcc cattactgct tgcatgcgcg cgaacactgc
    67681 tagtcattcc tgttttgatt ccgtcataaa tagtatacgc atcgattcct agatatttga
    67741 caatatatga acggtcaaat gttcgctgca gcaattgtgg acggttggct tcgctcagcg
    67801 ctagatttcc tgaaaaaata agccctgaaa gacaggtaac agcgatagcc atcggtttgc
    67861 cgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga
    67921 acacagcgat atccagccag tagatgacat catggggctt catcaaagaa aaggagcttc
    67981 ctgacaatcc ttgcgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg
    68041 tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga
    68101 tcaaaagaat agctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca
    68161 ataaggtagt cgcaattggg ttgatccaca agataaagta ctggatcgta ccttccacac
    68221 ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg
    68281 tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt
    68341 ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt
    68401 gttatcttaa gattttttta ttctttctaa agattattcc gtccactttt cacagcataa
    68461 aaccgctgcg atgtaagcga ttaaactaat gaagagataa aagtcttgtg gtgaccgctt
    68521 cactccacgg tattcaaaag ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca
    68581 aaattcttgt cgcagctcct tatttcttga tgcaggacag ctttttcaca acttcttcct
    68641 taaacggtgt tctcctgcca actcctcggc cacaagtcac aatatttata aaacatgaga
    68701 agctgttttt tgaaattg
//
LOCUS       DACECJ010000005        67598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.005, whole genome shotgun sequence.
ACCESSION   DACECJ010000005 DACECJ010000000
VERSION     DACECJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.005"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(29..985)
                     /locus_tag="IWO09_000300"
     CDS             complement(29..985)
                     /locus_tag="IWO09_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0262923.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1159..1719
                     /locus_tag="IWO09_000301"
     CDS             1159..1719
                     /locus_tag="IWO09_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262924.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1712..2536
                     /locus_tag="IWO09_000302"
     CDS             1712..2536
                     /locus_tag="IWO09_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0262925.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2546..3163
                     /locus_tag="IWO09_000303"
     CDS             2546..3163
                     /locus_tag="IWO09_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0262926.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3181..3864
                     /locus_tag="IWO09_000304"
     CDS             3181..3864
                     /locus_tag="IWO09_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0262927.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4547..5782
                     /locus_tag="IWO09_000305"
                     /pseudo
     CDS             4547..5782
                     /locus_tag="IWO09_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            5790..6677
                     /locus_tag="IWO09_000306"
     CDS             5790..6677
                     /locus_tag="IWO09_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0262928.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6694..7530
                     /locus_tag="IWO09_000307"
     CDS             6694..7530
                     /locus_tag="IWO09_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0262929.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            7533..8156
                     /locus_tag="IWO09_000308"
     CDS             7533..8156
                     /locus_tag="IWO09_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262930.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8153..9445
                     /locus_tag="IWO09_000309"
     CDS             8153..9445
                     /locus_tag="IWO09_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0262931.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9804..9901
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10017..10598
                     /locus_tag="IWO09_000310"
     CDS             10017..10598
                     /locus_tag="IWO09_000310"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0262932.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10601..11908
                     /locus_tag="IWO09_000311"
     CDS             10601..11908
                     /locus_tag="IWO09_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0262933.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12201..12689
                     /gene="purE"
                     /locus_tag="IWO09_000312"
     CDS             12201..12689
                     /gene="purE"
                     /locus_tag="IWO09_000312"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0262934.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12673..13806
                     /gene="purK"
                     /locus_tag="IWO09_000313"
     CDS             12673..13806
                     /gene="purK"
                     /locus_tag="IWO09_000313"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0262935.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            13819..15114
                     /gene="purB"
                     /locus_tag="IWO09_000314"
     CDS             13819..15114
                     /gene="purB"
                     /locus_tag="IWO09_000314"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0262936.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15294..15671)
                     /locus_tag="IWO09_000315"
     CDS             complement(15294..15671)
                     /locus_tag="IWO09_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262937.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            15927..16655
                     /locus_tag="IWO09_000316"
     CDS             15927..16655
                     /locus_tag="IWO09_000316"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0262938.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16655..16909
                     /gene="purS"
                     /locus_tag="IWO09_000317"
     CDS             16655..16909
                     /gene="purS"
                     /locus_tag="IWO09_000317"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0262939.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            16911..17582
                     /gene="purQ"
                     /locus_tag="IWO09_000318"
     CDS             16911..17582
                     /gene="purQ"
                     /locus_tag="IWO09_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0262940.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17583..19805
                     /gene="purL"
                     /locus_tag="IWO09_000319"
     CDS             17583..19805
                     /gene="purL"
                     /locus_tag="IWO09_000319"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0262941.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19790..21229
                     /locus_tag="IWO09_000320"
     CDS             19790..21229
                     /locus_tag="IWO09_000320"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0262942.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            21261..22304
                     /locus_tag="IWO09_000321"
     CDS             21261..22304
                     /locus_tag="IWO09_000321"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0262943.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            22301..22879
                     /gene="purN"
                     /locus_tag="IWO09_000322"
     CDS             22301..22879
                     /gene="purN"
                     /locus_tag="IWO09_000322"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0262944.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            22876..24411
                     /gene="purH"
                     /locus_tag="IWO09_000323"
     CDS             22876..24411
                     /gene="purH"
                     /locus_tag="IWO09_000323"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0262945.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24427..25701
                     /gene="purD"
                     /locus_tag="IWO09_000324"
     CDS             24427..25701
                     /gene="purD"
                     /locus_tag="IWO09_000324"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0262946.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25823..26737
                     /locus_tag="IWO09_000325"
     CDS             25823..26737
                     /locus_tag="IWO09_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0262947.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(26870..28435)
                     /locus_tag="IWO09_000326"
     CDS             complement(26870..28435)
                     /locus_tag="IWO09_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0262948.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28428..29879)
                     /locus_tag="IWO09_000327"
     CDS             complement(28428..29879)
                     /locus_tag="IWO09_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0262949.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(29893..30909)
                     /locus_tag="IWO09_000328"
     CDS             complement(29893..30909)
                     /locus_tag="IWO09_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0262950.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            31129..32217
                     /locus_tag="IWO09_000329"
     CDS             31129..32217
                     /locus_tag="IWO09_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0262951.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            32271..33797
                     /locus_tag="IWO09_000330"
     CDS             32271..33797
                     /locus_tag="IWO09_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ0262952.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            33799..35004
                     /locus_tag="IWO09_000331"
     CDS             33799..35004
                     /locus_tag="IWO09_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0262953.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            35261..36238
                     /gene="guaC"
                     /locus_tag="IWO09_000332"
     CDS             35261..36238
                     /gene="guaC"
                     /locus_tag="IWO09_000332"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0262954.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            36502..37926
                     /locus_tag="IWO09_000333"
     CDS             36502..37926
                     /locus_tag="IWO09_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0262955.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            38388..39497
                     /gene="pdhA"
                     /locus_tag="IWO09_000334"
     CDS             38388..39497
                     /gene="pdhA"
                     /locus_tag="IWO09_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0262956.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            39500..40477
                     /locus_tag="IWO09_000335"
     CDS             39500..40477
                     /locus_tag="IWO09_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0262957.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            40502..42145
                     /locus_tag="IWO09_000336"
     CDS             40502..42145
                     /locus_tag="IWO09_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ0262958.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            42153..43559
                     /gene="lpdA"
                     /locus_tag="IWO09_000337"
     CDS             42153..43559
                     /gene="lpdA"
                     /locus_tag="IWO09_000337"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0262959.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            43721..43996
                     /locus_tag="IWO09_000338"
     CDS             43721..43996
                     /locus_tag="IWO09_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0262960.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            44002..44769
                     /locus_tag="IWO09_000339"
     CDS             44002..44769
                     /locus_tag="IWO09_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0262961.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            44937..46766
                     /gene="typA"
                     /locus_tag="IWO09_000340"
     CDS             44937..46766
                     /gene="typA"
                     /locus_tag="IWO09_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0262962.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            47027..48799
                     /gene="recQ"
                     /locus_tag="IWO09_000341"
     CDS             47027..48799
                     /gene="recQ"
                     /locus_tag="IWO09_000341"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0262963.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            48913..49215
                     /locus_tag="IWO09_000342"
     CDS             48913..49215
                     /locus_tag="IWO09_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0262964.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            49218..50381
                     /locus_tag="IWO09_000343"
     CDS             49218..50381
                     /locus_tag="IWO09_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0262965.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            50407..53835
                     /locus_tag="IWO09_000344"
     CDS             50407..53835
                     /locus_tag="IWO09_000344"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0262966.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            53856..54980
                     /locus_tag="IWO09_000345"
     CDS             53856..54980
                     /locus_tag="IWO09_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262967.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            54970..55407
                     /locus_tag="IWO09_000346"
     CDS             54970..55407
                     /locus_tag="IWO09_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0262968.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            55391..55729
                     /locus_tag="IWO09_000347"
     CDS             55391..55729
                     /locus_tag="IWO09_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0262969.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            55848..56408
                     /gene="rsmD"
                     /locus_tag="IWO09_000348"
     CDS             55848..56408
                     /gene="rsmD"
                     /locus_tag="IWO09_000348"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0262970.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            56405..56896
                     /gene="coaD"
                     /locus_tag="IWO09_000349"
     CDS             56405..56896
                     /gene="coaD"
                     /locus_tag="IWO09_000349"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0262971.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            56886..57929
                     /locus_tag="IWO09_000350"
     CDS             56886..57929
                     /locus_tag="IWO09_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0262972.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            58088..58816
                     /locus_tag="IWO09_000351"
     CDS             58088..58816
                     /locus_tag="IWO09_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0262973.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            58932..59177
                     /locus_tag="IWO09_000352"
     CDS             58932..59177
                     /locus_tag="IWO09_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0262974.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            59174..59392
                     /locus_tag="IWO09_000353"
     CDS             59174..59392
                     /locus_tag="IWO09_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0262975.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            59528..60067
                     /locus_tag="IWO09_000354"
     CDS             59528..60067
                     /locus_tag="IWO09_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0262976.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            60060..62333
                     /locus_tag="IWO09_000355"
     CDS             60060..62333
                     /locus_tag="IWO09_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0262977.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            62371..63417
                     /gene="holA"
                     /locus_tag="IWO09_000356"
     CDS             62371..63417
                     /gene="holA"
                     /locus_tag="IWO09_000356"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0262978.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(63506..63760)
                     /locus_tag="IWO09_000357"
     CDS             complement(63506..63760)
                     /locus_tag="IWO09_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0262979.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(64160..64429)
                     /locus_tag="IWO09_000358"
     CDS             complement(64160..64429)
                     /locus_tag="IWO09_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262980.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(64899..65354)
                     /locus_tag="IWO09_000359"
     CDS             complement(64899..65354)
                     /locus_tag="IWO09_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0262981.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(65492..67420)
                     /locus_tag="IWO09_000360"
     CDS             complement(65492..67420)
                     /locus_tag="IWO09_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0262982.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        21970 a        11609 c        14799 g        19220 t
ORIGIN      
        1 agaacagctt tttcacaacc ttttaaaatt acatattttt ccatagaact tgcatttgat
       61 ccaagatttc gatgacatct tttgtcaaga agagagagcg attgggcaag tcaccttcaa
      121 tcatgtgtac catattcttt atttcgtaat tcattgcttc agatgtattc ccgctttcga
      181 tgaattcctt tgtaccatct gtaaataaga tctccgcacg atcagcacgc gggtaatcag
      241 caatcgtcac ataaccgttc tcaaaggcaa cgataccgac ttttggcatt tttgcctgaa
      301 aagtcaagct gacagcagca agttcatttt ctttgttacg caagatagtg acggattgtt
      361 cgtccacgcc tgtttcaaaa ggaaccattg tagaggcaac tacttcaggt tgagagctca
      421 aaaagaaacg agcgaaagat acagcatacg taccgatatc caacagagct cctccagcta
      481 attctggatt gaagaaccgg tttttagggt ctggttcttt gtaactgccg aaaggtgctt
      541 ggatcatttt taaagccccc agttttcctg tatccattat tgagcgtaac tgctgataaa
      601 gtggcatgtt gaagattgtc atcgcttctg ctaagatcaa attcttttct tctgctaagc
      661 gcatagcgtc agccagttct tttttattca tcgtgatggc tttttcacat agaacgtgtt
      721 tgcctgcttc caatgctgcc aagatatggt cgatatgctg tctatttggt acagcaatgt
      781 acacgatgtc gatttcagca tcgtctaaca tttcttgata gcttccgtaa gcttttggaa
      841 tattgtaacg gtgagcgaag ttttccgctt tttctgaagt tctggaagca actgctgtta
      901 gttcgctcgt ctcttgattg aagctttcgg caaactcatg tgcgatggta ccaagaccaa
      961 cgattcccca ttgatatttt ttcatctgaa aacgctcctt catagttgga ttgagtttct
     1021 tgtctgtcta ttattataac aaaaatcacg aagaataaag aggagaggac ttgaaacatt
     1081 aaaaaaaatg aaagcagcga tttaagaggt gctttttgat caaaacttaa gtagaatagt
     1141 taaaaggagg ggataaacgt gcatcaagta tatattgatc ttattatgag tgtattatta
     1201 gaacagtttg aaactgaaaa aaatttttta gatgaatact tgctgatcag tccagaacaa
     1261 tggcaaaaat ggaaagatgg cgagaaaaat ctgccatctg aagcgatgca gaaagtaaaa
     1321 aatcttttca ctgattatga atggatgcta acgcaaaaga ttttgcgtca aacgattatt
     1381 tttcccgaaa aacgaaacgt tgccgttgca gagtacaagc aaatcaaaac acgcatcgcg
     1441 caaaaatggc ttagcagcaa agcagcaaaa gtcgagctga tccctttgaa aaataatcag
     1501 caggctggta gataccttga cttgaaagta agtattcaat atgatgaatg gggctatgat
     1561 gatattttga attttcgtct gccagcagat gttcaaaaac agatcgaagg agaaaaaatc
     1621 gaacttctcg attgggtgaa tgaaaatctg gaagaaactt acgttcatga ggcgaatggg
     1681 gaacgtgaag aacaacgaaa ggatttttca agtgaataaa tttttaggat ttggagcagt
     1741 tgtgattatg atcgcagcaa tgggctatgt tatttttagc gatgatgatt cgcatgttca
     1801 agccggcaat caagcagtcg aacgaacaag caccaaaaca actgctagta aagaaaagaa
     1861 cagtcaaaca accagcagca aggtaaagga caaagaaaca aaagatcgta cgtccaaaca
     1921 attgccagat gttaaaacag acgattggaa gctagttttg gttggtccag atcacaaatt
     1981 agaaaaagaa attgatgagt ctactcaatt ggcatcatta agtaacggat acttgatcga
     2041 caaacgcatt gctccagaat atgaagcatt tgccaaggct gctgaagcag ctggatttcc
     2101 attagtgatg gtctcagctt accggtctgt atcatcccaa caacaagttt tcgaacaaaa
     2161 tgtccaagat gtgatgagtc gtcaaggtgt atcagaagag gaggcgacaa agattactaa
     2221 agaaacgatt acggagccag gatacagcga acaccacaca ggtctggcag tcgatgtcgt
     2281 cgatcaaaac tggtacaaca gttatccttc tactgtattg gatgcaagtt acggagatca
     2341 gcctggagct aaatggatcg cagataatgc tgcgcagtat ggatttatcg ttcgttatcc
     2401 aaaaggacac gaagatataa ctaagatcac atatgaacct tggcacttac gttatgtcgg
     2461 taaagaaaac gctgagtata tcaccaaaca ccaattgacg ctagaagaat atctgaatga
     2521 attaaatgaa aagtaggaat tgatcatggc gaaaaaaaag tggaaaaagc aaaagcgcaa
     2581 gaatatccgt tggccagcag tggttgccgg tgtagcagtg atactcttag cctttgtttt
     2641 ttccttgaag aatctgtctt ctccttttac tgataatcag caagaaaatg aagcgcaatc
     2701 ccatcagcaa tttattgaac ggcttgttcc gcatgcccaa gaactacaag atggttatgg
     2761 agtgcttcct agtatcattt taggacaagc tatattagaa tcaaattggg gaaaaagtac
     2821 cttggctagc aaatacaata atcttttcgg gatcaaggct tatggggatc agaagaaagt
     2881 aagcctggaa acaaaggaat ttgttaacga agaatggata actatccaag gagacttcaa
     2941 agtgtatgat tcatgggaac agtccatgga tgaccataca caactgttcg tccaaggagt
     3001 agattggaat cctgcgctgt atgaaaaagt gattacggcc accaactatc aggaagccgc
     3061 acaagcattg caagatgcag gttatgcgac agaccccggt tatgcgcaaa aaatcatcca
     3121 ggtcatcgag acgtatcaat tgaatcagta tgaccggaga tagcaaagga gaagtggctg
     3181 atggcagaac aatttacgcc agaaatcatg acggaattga gaaaagatat cgtaagagta
     3241 ccggaaatca ttttagaagc aagcgggatc aagatatttg gaaaattgat ccgttcgatc
     3301 atctttacta cggatattgc gatcatacgt aatacaaatg cgaatgcagt aattgctgtg
     3361 tatcctttta cacctcatcc agcgattact aaaagcatta ttgaggcagc tgacattcca
     3421 gttttctccg gtgtaggtgg tggtttgaca caaggtgctc gttcttccta catgagcatg
     3481 ttcgcagaag cgcaaggttc gttaggggtc gttttgaacg gtcctacgcc tttagagaca
     3541 gtaaaagaag tttgtcgagt agttgatatt ccagttatca gtacggtaac gtcgaaatat
     3601 acagaaatag atgaaaagtt agcagctggt atttcgatta tcaatatcag tgcaggtaaa
     3661 gaaactgcag aagcagtacg ccattttcgt aaaaaatatc ctgaacttcc tatcattgcg
     3721 acaggtggtc caacggaaga aagtattgct gagaccattc aagcaggagc aaatgccatc
     3781 acatatacgc caccaagtag cggagagctt tttagtcgta aaatggaaaa atatcgggaa
     3841 actgagaaaa agaatcatga ataacaggtg agaatcaaaa gacgaggaag agcagatagt
     3901 ggctcttcct cgtcttttgt actccagtac tatttgttca gaattcaaaa aaaagagaaa
     3961 tttttttgtt tttttacctg taatgtacat aagaagagtt aaaacctttt gttattatta
     4021 gcattctaac aaaaaaatgt cttgatttat tgaagcgctt tctatagaat gaaaaacgaa
     4081 taaaaactaa aaaaggcacc cgacaattta gtagcagcta aattgtcggg gcttgaaaag
     4141 tcagcgggaa ctgaccaatc aagttgccat agtcaatgct tgtgcatcga cgtgtatatt
     4201 gtacctaatt tcatacagaa ttactagtta tttttctgtt ttttagggaa gagcgcacac
     4261 gaagaagcat gcttatttag ctatgtcaag gatattgggc agttgtctga tatccttttt
     4321 tctttgcgta attatcctcc catcattgaa gcaagaagtg tttttagcga tgaaagaaga
     4381 atcagcacta tctaattttt cttgaaagta ttgagttgtt catcctaggc agaacgataa
     4441 ggcaagtctt ggtttttatt ccccttgaaa aagatcgttt aataaatatg ttctgtataa
     4501 aaatacagta tattgaaaaa taacaatgga agtagaggtg cagagaatga agaaaagaat
     4561 gagaatcatc ttagctttaa gtgttatgtt attcttcttg acggcttgtg ggcaagaaga
     4621 tgacaaaaaa atcacgctga acatggcttg gtggggttcg caagtccgac acgatgcaac
     4681 agtaaaagta atcgagcttt atgaagaaaa aaatccccat gtgacgatcg attatgaatt
     4741 ttatgattat gaaggatatt acacaaaatt aggtacacta gcagcatcag atgatatctg
     4801 ggacattttc caactgaaca atcaatttcc tcaatacatc aatcagattg aaccattaga
     4861 tgaatatgtg gagcaaggga taattgatat ttctaatgca gatgaatcct atttagcaac
     4921 gacgacttac gacgggcaat tagttggact gtctaacggg gtgaattcgt ttgcgattgc
     4981 ttacaataaa aatatatttg aacaattaaa tatccctgaa ccagcttcaa attggacatg
     5041 ggatgaattt gaacaaatca gctatcaaat cactgaagaa ttaggaatct atgctatttc
     5101 aagattcgaa gattttatag ctggctgtat tattcaaata ccacaagttg agaaaggatt
     5161 gaatttttat aatcgggaag atctatctaa cagcttggga tttgaaaatc cagactatct
     5221 aacagatttt ttccagctgc gtaaaaatct ggtagacaaa ggggcccatc cagaaccagg
     5281 tggtggagct gcaagtacga cggatctgtc ttttcaggcg gttagggata gagaagcggc
     5341 aatggtgttt ctatcaagta accagttgac agaaatattg tcaggggctc ctgaagatat
     5401 ggagattcag ttgattaatc cacctcgcag aaaagcagat ggagaatctg gtatcccttt
     5461 acgttcttcg caaatgctga gtatggcaaa aagctcagcc cataaagaag aagctgctaa
     5521 gtttattgat ttctttcaaa acagtgaaga agccaatgaa attttaaaag gagaacgtgg
     5581 tgtaccaatc atgtcgaata ttcgagaaca gctcgctcgt gcagatgatg caactttgca
     5641 agcgacattt gattatttag aaatgatttc cgatatggac aacggcgaag tgaatgtctt
     5701 tgatagtgct ttgaatccgg aaattcaaga ccaatacaat cttttagttg aaaaagtcat
     5761 ttatgaagaa gtaacagcag aaggaaggca tgaaaaaaga aaaactaaaa cggttcttgg
     5821 atcaagacaa tgtggtcggt tacgtatttg cttctccatt catcctagga tttctattga
     5881 taacagtgat accgatgatc ttgtcacttt attattcgtt caccgattat cgtttgggaa
     5941 atccagtctc atggatcggc ttggataatt atataaagat gctgagtgat gcccgttttt
     6001 tgaactccat ccgagtcact tttctctatg tatttgcatc tgtacctgtg aaactgatct
     6061 ttggtctatt gattgcttat cttttaacga ggaatatcaa aggaacgact ttctttcgtt
     6121 ctttgtatta tgtgcctagt ttgatcggtg gcagtatttc tatcgctcta gtttggcggg
     6181 agttgttttc taaaaacggg ttagtgaata ttttcctgga acgattaggc gcacctgcgc
     6241 tttcctggtt tagcgatcaa aaactggtca tgatccctct tgttttgatg tctgcttggc
     6301 aattcggttc ggcaatgatc atctttgctg caggtatcaa agagatccca ggctcgtatt
     6361 atgaagcggc agagatcgat ggagcgaaca aatggcagac gttttacaag atcacgttac
     6421 ctcttctttc gccgatcatt ttgtataact tggttatgca gacaatcgcg gcgtttatga
     6481 cttttacgca ggcatttatc attacccaag gtggtccgaa tgatgccacg aacttttatg
     6541 ctctttatgt gtataaccaa gctttttctt ttaattctat ggggtatgct agtgctattt
     6601 cgtggatcat gctgttgatt atgggagctg tcacctttct gattttcaaa tcatcaaaaa
     6661 aatgggtcgt tgtgtgaatg agaggaggaa gaaatgaata atacagtaac gaaatggttt
     6721 caatacggtt ttattttgct attaggtcta gtcatgattt atccggtctt atggatgatc
     6781 gcaggttctt ttaaaacgga tcaagagatt atgagtggtt cgctgaattt gattccagag
     6841 acttttcaat gggaaaatta cgcaaaagga tgggcggggt ttgctggaat ttcatttttt
     6901 actttcttta ggaattcgtt tgtcatctct gcagtttcga cgatagggac agttctgagc
     6961 tcgacttgta ttgcttacgc attatcgaga atcgatttca aaggaaaaag attctggttc
     7021 gtcgtcatgt tagtaacgat gatgattcct gcacaagtga ttttgattcc gcagtttatt
     7081 atttacaatc ggctgaattt ggttgggacg tatgtgccgc tgattttacc acattttttt
     7141 ggacaagcat ttttcattta ccagatcatg caattcatgg tcagcatccc caaagagtta
     7201 gatgaatcag cgattattga cggatgcagc aagtatagtg tctttaccaa aattattttt
     7261 cctttgctga aaccttcgat catcacgacg atcatcatcc agttttattg gaaatgggac
     7321 gacttcatgg gaccattgat ttatttaaac aagccgagat cttacacggt atcgattgcc
     7381 atcaaattat ttgcagacgc tggatctagt acgacttacg cgagtatgtt tgcgatgtct
     7441 acgttgtcgc tgctaccggt ttttctgatt ttcttattct tcaataaata tcttgtccag
     7501 gggatcagta caagcggtct gaagggctga atatgaaaaa gccaatagaa gagatgtttc
     7561 tttacaagct caactactat gtctgtcgga tttttttaac cagtcttatc agtaatgctc
     7621 ttttcgcttt agttctagga tgtttgttca ctttaggatt agtgctgtct gctcgtgttg
     7681 tctatctgat ttattttttg atgatgtatc cttcttatta tctatctgtc cattacttgg
     7741 cagaaaaaaa ggtatgttgg aggaaaggga aaatcaaacg gatgtgtttc atcattacgt
     7801 tgtttcagct gtttgtcatt atttttgcag ttgattggta tttattagtg ggaaagattg
     7861 gagcttggta tctgattcct tttctattgt gtctgttgct ttttgtatcc catagttttt
     7921 ttagtacgct aattgatatc tgcaaagaag ataaagcaga tagtcttgtt tatttgatca
     7981 aaaggaatat ctggctgttg tcaaaaaacg tcaaggggtt tctgtggtat ttatgccttt
     8041 tgtatggact gttctggtgt atacagcctg attttttact cactgcatta ctgtatccag
     8101 ggctgccatt tgtcacagta ttttgcttaa aagaaaagga gaatgacttt aaatgaagaa
     8161 aaaatatgta caagtcggac ttggtgggag agcgcgtttt ttttatgagg caattgcctc
     8221 tacgtatcat caaacttgtg aactagtcgg tttttgtgat gtatctcaaa caaggatgga
     8281 ttatgcaaat cggatacttg tgaacaagta tggacagaaa aaagtaaaga catgtatgcc
     8341 atcacaattt gagcaactac tggatgacgt acggccggct gaagtcatta ttacgtcagt
     8401 agatcgaacg catcatgatt atatcattcg tgcaatgaaa aaaggatatg atgttatttg
     8461 cgaaaaacct ctgactatcg atgaggaaaa agctcaagaa ataataacgg tccaaaaaca
     8521 gacgggaaga agcctgcgag tcacttttaa ttatcgttat gcaccgtatc acacgaagat
     8581 aaaggaactg atagaaagtg atgtgattgg tgatgtgttt tctatccact tcgaatggct
     8641 gttaaatacg caacacgggg cagattattt cagaagatgg catcgaaata agcacaatag
     8701 cggcgggctt cttgttcata aatctaccca tcattttgat ctagtgaatt tttggttgaa
     8761 cagccgtccc caaaaggtat ttgcattcgg tggattaaac ttttatggga tgcataatgc
     8821 ggagaaacgc ggagtagaaa tattttacag tcgatgctat ggaagcgaac tagctaaagc
     8881 tgatccattt gctatagacc tgtcagccaa tgaggaattg cgagaaatgt atttagaagc
     8941 agaaaaagac agtggatata ttcgtgaccg cagtgtattt tccgatgata tctctattga
     9001 agatacacta ggcgtaacgg tcaaatatga aactggtgcg attttgacgt attcgctaaa
     9061 cgcgtatatg ccgtgggaag gattcaatgt ttctttcaat ggcagcaaag gacggatcga
     9121 aatgaaagtg atcgaaaagt cttacatcaa tgcaggtgga gataaaaagg atgaaggagc
     9181 agccgttctt cagtccatcc atgtctatcc gatgttcggt gagccatatg aggtaatcgt
     9241 cgaacaagaa gcgggcggac atggcggagg agatccgcgt ttattagatg atctatttgg
     9301 tgaagaaaaa gaagaagatc cgtggaatcg ggcagcaact cacgtagatg gtattttgtc
     9361 gattttgaca ggaatcgcag caaatcattc tattgcttca ggaaaagcag tagtgattga
     9421 cgagcttgtc agcttaaaag aataagaata gatttacgga aattacttcc cgtagtttat
     9481 aaaatcttgc cttattgccg ataggctgtc ttttgaacac tatttattcg gaaaaagggg
     9541 ctatgacatg ctttttgtca tgccttcttt ttatgtgggc tgctttttta gataggagaa
     9601 aataaaaata actatttaaa cccgaacaaa aaggaataat gcattaaaaa tcattagatt
     9661 ttacaaaaaa ataagtaaac cacgttgtca gtttcagaaa gtagtgttag actgttttca
     9721 atatttgaaa caacattttt tattcaaata tggcaaactg ccttcacgtg caaaacagtt
     9781 gcaagaaaat gaatttacac tattattagt tttacatacc tacttatata tcgtcataat
     9841 atggatgaca gtttctagcc agtaccgtaa atgctggact ataagtaaaa gattggctat
     9901 ttcacccttc ttttcttatg ataaagtgga gggtgttttt ctttaacaga aacatagtag
     9961 gtttttgcac aaaagcggtt atttcggtat taataaatga taaaggagca aataaagtga
    10021 aagaattagt tgaacgtatt caaaaagacg gacgtatatt aggcgagggc gtattaaaag
    10081 tcgacagctt cgtcactcac caagtagacc ctgtattgat ggaacaaatg ggaaaacggt
    10141 ttgcagaagt atttaaagaa caaggaatca cgaaagttgt gaccattgaa gcttctggga
    10201 tcgcaccagc tttatttgct gcaagagaat tagatgttcc aatgatcttc gctagaaaag
    10261 caaaaagttt gacgatggat gaagaattat tgacagcttc tgtttattct tttacgaaac
    10321 aagtgacaag tacgatttct atttctcgta aatttttatc ttcagaagac aaagtattga
    10381 ttatcgacga tttcttggca aacggacaag cggcaaaagg attgatcgaa ttatgccagc
    10441 aagctggagc gcaagtagaa ggaatcggga tcgtcatcga aaagtctttc caagatggac
    10501 gtcagttact tgaagaaatg ggattgagag tcgtttctct tgcgcgtatt gcttccttga
    10561 cgaatggaca agttgagttt cttgaggagg acgcataatc gtggaaaaag aaacacaaaa
    10621 tggaaaagct gcggttttag ggctgcagca tctgctagcg atgtatgcag gagcagtagc
    10681 ggtgcctctt ttgatcggta caggactagg ctttaatcaa gaacagatga cttatttgat
    10741 ttcaattgat atctttatgt gtggattagc gacattattg caattgacag ttaaccgatt
    10801 tttcggaatt ggcttgcctg tcgttttagg atgtgcgatc caagcagtag ctccactgat
    10861 cttgatcgga accaatgaag gagtaggagc tatctacgga tctattatcg cttctgggat
    10921 ctttgttgtg ttagtttctg gtgtcttttc taaaattaaa aaactattcc ctcctatagt
    10981 aacaggaacg gttatcaccg ttattggttt gactttgatt cctgtagcaa tcgagaaaat
    11041 gggtggaggt aatgcaacag cagctgattt tggtgacaaa acaaatctgc tacttgcatt
    11101 tgtgacgatt ctcctaattg taggcgtgca aatgctggca aaagggttca ttcgttcaat
    11161 cgcggtattg attggtttag tcggaggaag catcttagct gctttcatgg gaatgattga
    11221 tctgagcgcg attggagatg cgccgttgtt ccatgttcca caaccctttt attttggtaa
    11281 accaactttc gacgtatggt cgattttact aatgatcatc atttccatgg tcagcatggt
    11341 agaatcaaca ggtgtatatt ttgcacttgg tgacattact gggaaaaatg ttggagaaga
    11401 agaattgaaa aagggatatc gtgcagaagg acttgccgtt atcttaggcg gtattttcaa
    11461 tacattccct tatactggct tttctcaaaa tgtcggattg gttcaacttt cgggaatcaa
    11521 aacaagaaaa cccatctatt tctctgcatt cttcttgatt gttttaggat tattccctaa
    11581 aatcggtgcg ttagcacaaa tcatccctga gccggtactt ggtggaggaa tgttagtcat
    11641 gttcgggatg gtggcagtcc aaggaatccg tatgctgttg gaagttgatt tcagcaatga
    11701 taaaaatcta ttgattgttg ctgtttctgt tggatttggg ctgggtttca atgttacgcc
    11761 gacattattc agtcaaatgc cgcaaacatt gcaaatgttc actggaaatg gcatcgtaat
    11821 gagtagcatt acggctatca tattgaattt gatctttaat ggattgaaaa aagatgaaga
    11881 aatattggaa gaagccttca agaagtaata gagcaaaaga aaggttgtga aatcagcgtt
    11941 ttgacctgat tgttagagaa acaaacggaa aaatagttcc tcgtattttg ccgtttgttt
    12001 gagctaatac cgagggtcag ctgattgaac accgtttatc cataaaaagg aatgtgacct
    12061 aacttttgtc acattccttt ttttcatctt taataaacac gaacaataaa atgtttttct
    12121 ttgttaacat tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat atgaatgtag
    12181 aaaaactggg aggaaaaaca atgacagcaa aaatctctgt gattatgggg agtacttcag
    12241 attgggaaac aatgaaagaa gcatgtcaaa tattggatga attcggtgtg tcttatgaaa
    12301 aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt tgaatatgca caaaacgctc
    12361 gaaaaaaagg aatcaaaatc attattgcag gagcaggggg agccgcgcat ctgccgggta
    12421 tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc ggtccaatca cgaacattaa
    12481 atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct gtagctacta
    12541 cagcaatcgg aaaagcggga gcaattaatg ctggactact tgcgatacag atgctttcca
    12601 tgtatgattt agaattggag gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg
    12661 ttatagaaag tagtgatcaa cttggttaaa cctttgttgc ctggttcaac gatcgggatc
    12721 gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg caaaagaaat gggctttaaa
    12781 gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac aagttgcaga ttggcacatt
    12841 gaaagtgtct atgatgacac ctttgcgctg aaagagcttg ccagaagaac agatgtcatt
    12901 acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc catgtcattt
    12961 attcctcaag ggacagactt gcttgcgatt acgcaagacc gcctgttaga aaaatctttt
    13021 ttggaaacga ataacatcgt gattgctcct tatgcaacga tcgtcagtcc gacggatatc
    13081 caagatgcga ttgacgggat cggctatcct tgtgttttga aaaccactcg aggcggctat
    13141 gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc catggacctt
    13201 ttaagagaag gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga gatttcgatc
    13261 atggtggcag gaaatggtca aggagatttc acgacttttc cggtcgtgga aaacatccac
    13321 cacaacaata ttcttcatga aacgattgct ccagccgcga ttgatcagga tgtgatcgag
    13381 gaggctgagc gtattgcccg tgtgattgca gaagcagtga gcctttcagg tgttttaggg
    13441 gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga atgaattagc tcctcgtcct
    13501 cacaattctg gacattattc tattgaagct tgttctatga gtcagttcga tgcccatatt
    13561 cgaggtattt gtggttggcc attaggagaa aaagtacgtt tgataagcaa tgcagtaatg
    13621 gtcaatgttt tgggagaaca actgctggaa agctatcgct tgattgagaa taagccagac
    13681 tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc
    13741 actatcttga cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg
    13801 gattagagga gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg gaatatctgg
    13861 acggaagaga acaaataccg tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg
    13921 gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt
    13981 gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca
    14041 cgagcggttt ctgaaacatt gggagaagaa agaaaatggg tccattatgg tctaacaagt
    14101 acggatgtcg tagatactgc ttatggctat ctcatcaagc aagcaaatga tattttacgg
    14161 gaagacttga aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca caaatataca
    14221 gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa ctacttttgg attgaaactt
    14281 gccacatggt attcagaaat gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga
    14341 gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg caaatatccc gccatttatt
    14401 gaagagtatg tatgtaaaca attggggatc cgtccacaag aaatctctac tcaagttctc
    14461 cctcgggatc tacatgctga atatttctca gcgatggcgc ttattgctac aagtattgaa
    14521 cgttttgcaa cagagatacg cggattgcaa aaatccgaaa cacgtgaagt agaagaattt
    14581 tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc gatcggttca
    14641 gaaaatatgg ctggtctagc cagagtggtg cgcggacaca tggtcactgc ctatgaaaat
    14701 gtcacattat ggcatgaaag agatatttct cattcttcag cagaacgaat catcttgcct
    14761 gataccacga ttttattgaa ttacatgtta aatcgttttg gcaatatcgt taaaaatcta
    14821 acggtctttc ctgaaaacat gaagcggaat atgtctgcaa catttggttt gatttatagc
    14881 cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa gagaagaagc ttatgatctt
    14941 gttcagccaa aaactgcgta cgcttgggac aaccaagtgc agttccgccc gctgttagaa
    15001 gaagatccaa ccatcacatc ggtattatct aaagaagaaa tagacgatgc ctttgattat
    15061 catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga ataaagaaga
    15121 gtgagttgca aaattcaatt aaaatagaat agcaaaaata accaaaaaag aagcagatcc
    15181 gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag gaacctgatt
    15241 ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg
    15301 taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg
    15361 agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata atgatttttg
    15421 aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa
    15481 agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat
    15541 gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta
    15601 tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt
    15661 gcatcttcca tcggcaagtg cctcctcgaa cccattatcc aaatttttag ttaattctaa
    15721 tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt tcgtttctga
    15781 taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata
    15841 aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt
    15901 aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag
    15961 ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag
    16021 caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc
    16081 aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg
    16141 aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg
    16201 ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag
    16261 aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat gatccattta
    16321 tcaatgaaga gcatatctat ttcttacaac ttgcaaaacc ggaagaaatc agtgagctga
    16381 aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg
    16441 ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg
    16501 aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag
    16561 acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc
    16621 tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac
    16681 ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat
    16741 gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga
    16801 tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt
    16861 aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg
    16921 cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga
    16981 tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg
    17041 gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc
    17101 ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg
    17161 gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt gtactgagac
    17221 gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg
    17281 cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag
    17341 gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct
    17401 ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag
    17461 cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat
    17521 cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat
    17581 agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg
    17641 aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc
    17701 ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa
    17761 attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg
    17821 gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa
    17881 gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa
    17941 tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt
    18001 tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg
    18061 gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt
    18121 gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata
    18181 tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag
    18241 gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac
    18301 agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt
    18361 gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag
    18421 caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga
    18481 cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg
    18541 aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac
    18601 tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat
    18661 atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag
    18721 atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt
    18781 tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc
    18841 aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca
    18901 atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag
    18961 cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag
    19021 gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg
    19081 cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat
    19141 cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag
    19201 gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg
    19261 gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag
    19321 gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa
    19381 agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga
    19441 gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact
    19501 gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga
    19561 tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg
    19621 tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg
    19681 ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga
    19741 tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga
    19801 agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc
    19861 tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat
    19921 cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt
    19981 attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg
    20041 ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca
    20101 tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa
    20161 agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca
    20221 tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga
    20281 agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga
    20341 cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc
    20401 ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg
    20461 tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca
    20521 gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg
    20581 gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt
    20641 aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc
    20701 ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac
    20761 atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt
    20821 acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac
    20881 gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag
    20941 gattgcttct cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa
    21001 agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc
    21061 tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc
    21121 gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta
    21181 tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa
    21241 gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag
    21301 ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta
    21361 tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag
    21421 agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag
    21481 cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag
    21541 cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt
    21601 tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc
    21661 tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag
    21721 ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc
    21781 caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg
    21841 ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga
    21901 aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc
    21961 cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag
    22021 aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc
    22081 cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt
    22141 atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag
    22201 aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg
    22261 ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc
    22321 ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga
    22381 atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac
    22441 tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta
    22501 tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg
    22561 atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat
    22621 ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc
    22681 tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc
    22741 aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga
    22801 aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa
    22861 tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta
    22921 gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag
    22981 catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct
    23041 gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga
    23101 agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac
    23161 tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata
    23221 gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa
    23281 aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa
    23341 ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt
    23401 cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa
    23461 aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa
    23521 aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca
    23581 gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct
    23641 gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat
    23701 ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca
    23761 gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca
    23821 gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa
    23881 gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag
    23941 gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat
    24001 caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact
    24061 ccagcagaag caaaagcatt agcgtttgct tggagagctg tcaaacatgt gaaatctaat
    24121 gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga
    24181 gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta
    24241 gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata
    24301 aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat
    24361 aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc
    24421 ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa
    24481 cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca
    24541 aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt
    24601 gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg
    24661 atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct
    24721 ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca
    24781 gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt
    24841 gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc
    24901 acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat
    24961 agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg
    25021 tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt
    25081 gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt
    25141 tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg
    25201 aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa
    25261 ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt
    25321 tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta
    25381 gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac
    25441 cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt
    25501 tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat
    25561 ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta
    25621 atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat
    25681 agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa
    25741 tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt
    25801 atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc
    25861 agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat
    25921 tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg
    25981 tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct
    26041 ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca
    26101 tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt
    26161 acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga
    26221 attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat
    26281 cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt
    26341 gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat
    26401 cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg
    26461 gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga
    26521 attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc
    26581 ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt
    26641 agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta
    26701 cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa
    26761 gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg
    26821 tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt
    26881 tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa
    26941 ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac
    27001 gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat
    27061 cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg
    27121 caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa
    27181 aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag
    27241 cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga
    27301 ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt
    27361 tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag
    27421 ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata
    27481 atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt
    27541 cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta
    27601 tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa
    27661 tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta
    27721 cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga
    27781 atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat
    27841 aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata
    27901 cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac
    27961 tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa
    28021 caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt
    28081 ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta
    28141 cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag
    28201 tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt
    28261 ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac
    28321 catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt
    28381 cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg
    28441 tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg
    28501 tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg
    28561 tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct
    28621 ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat
    28681 tggatcatct catcaatttt gcatgcggta atacgaataa atcctttgtg acggacgtac
    28741 ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa
    28801 acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt
    28861 tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct
    28921 tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct
    28981 tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa
    29041 tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc
    29101 aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta
    29161 agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa
    29221 agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag
    29281 cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt
    29341 aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca
    29401 cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg
    29461 atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga
    29521 taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt
    29581 gtatttgtat tatttttgat ctctcgaata tcattcctaa gttcttctag gatattttcc
    29641 tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact
    29701 ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga
    29761 taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat
    29821 agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg
    29881 atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac
    29941 agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata
    30001 acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt
    30061 gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat
    30121 caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat
    30181 ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg
    30241 tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt
    30301 catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga
    30361 ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc
    30421 taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc
    30481 ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt
    30541 cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa
    30601 tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc
    30661 aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag
    30721 aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca
    30781 gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt
    30841 attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac
    30901 acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg
    30961 aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga
    31021 caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct
    31081 ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa
    31141 tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt
    31201 ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa
    31261 tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt
    31321 tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa
    31381 aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta
    31441 actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta
    31501 ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta
    31561 tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg
    31621 attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg
    31681 atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg
    31741 agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat
    31801 aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat
    31861 ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa
    31921 cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc
    31981 ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa
    32041 atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga aacttatgat
    32101 aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat
    32161 tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag
    32221 gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt
    32281 gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg
    32341 tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa
    32401 aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa
    32461 tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca
    32521 tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt
    32581 ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa
    32641 catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga
    32701 ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc
    32761 tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga
    32821 gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga
    32881 atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga
    32941 cactagaaaa tgaaatgatc agcgaagagc atattattcg cgggatggtg ggcagagatt
    33001 tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag
    33061 actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc
    33121 atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat
    33181 tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg
    33241 aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg
    33301 tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga
    33361 cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa
    33421 ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg
    33481 tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac
    33541 aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg
    33601 agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt
    33661 ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta
    33721 ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga
    33781 aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa
    33841 ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta
    33901 gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat
    33961 ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta
    34021 gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca
    34081 attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt
    34141 cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata
    34201 ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt
    34261 ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat
    34321 ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc
    34381 atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta
    34441 tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta
    34501 ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt
    34561 ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt
    34621 tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc
    34681 acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct
    34741 gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg
    34801 gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt
    34861 gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta
    34921 gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat
    34981 aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa
    35041 gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga
    35101 ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt
    35161 ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg
    35221 ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga
    35281 agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac
    35341 ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat
    35401 tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg
    35461 gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac
    35521 ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga
    35581 atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat
    35641 tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt
    35701 tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg
    35761 gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg
    35821 gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg
    35881 tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat
    35941 gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg
    36001 aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa
    36061 tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga
    36121 aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat
    36181 tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa
    36241 aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc
    36301 caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc
    36361 ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat
    36421 gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata
    36481 gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat
    36541 cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt
    36601 caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc
    36661 catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg
    36721 tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg
    36781 atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag
    36841 atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg
    36901 aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat
    36961 cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa
    37021 ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg
    37081 attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt
    37141 taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa
    37201 gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg
    37261 cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa
    37321 aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg
    37381 gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat
    37441 gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg
    37501 ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg
    37561 atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa
    37621 atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat
    37681 tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg
    37741 gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt
    37801 gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg
    37861 gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca
    37921 gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc
    37981 tgtttctttt tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt
    38041 tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc
    38101 aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga
    38161 tgaaaaataa aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat
    38221 tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc
    38281 atgataattt attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc
    38341 gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa
    38401 aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc
    38461 aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg
    38521 atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa
    38581 cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa
    38641 gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg
    38701 atgtacctca actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag
    38761 gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca
    38821 tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga
    38881 aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag
    38941 caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt
    39001 atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg
    39061 tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg
    39121 tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac
    39181 tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa
    39241 aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga
    39301 cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag
    39361 aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct
    39421 tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag
    39481 cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt
    39541 agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa
    39601 cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt
    39661 cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg
    39721 atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt
    39781 agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac
    39841 aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga
    39901 aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc
    39961 aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat
    40021 gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga
    40081 taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt
    40141 gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat
    40201 tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac
    40261 tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt
    40321 ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc
    40381 acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga
    40441 tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt
    40501 catggcttat caatttaaat tgccggatat cggtgaagga atcgcagaag gtgaaatcgt
    40561 caaatggttc gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca
    40621 aaatgacaaa tcagtagaag aaatcccgtc acctgttaca ggaacggtta aaaatgtgat
    40681 tgttccagaa ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg atgcaccagg
    40741 acacgaagac aatgaagggg acagtggtgt agccgcagaa tcccaaactc cagctaaacc
    40801 ggctgctgaa cccactgttg atacagaatc agctggatcg tcaagcgaag gagtcttcca
    40861 atttaagtta ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt
    40921 caaaccagga gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc
    40981 agtagaagaa attccgtcac ctgtcacagg gacagttaaa aacgtgattg ttccagaagg
    41041 cactgtagca aatgttgggg atgtattagt agaaatcgat gctcctggac acaatagtgc
    41101 accgtcaact agtgcgccat cagctgaagc accaaaagaa aaagtggaaa catcaggttc
    41161 agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta ttggctatgc catctgttcg
    41221 tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta actgcaactg gaaaaggtgg
    41281 cagagtaaca aaagaggaca tcgaaaactt cttggcaggt ggtccaagca gtgcaccagc
    41341 gaaatcagaa gcgccagaag cggcagcacc gaaagaagca gctcctgcag cagaaagcaa
    41401 accagctgca ccagcaaaac cattcaagtc caacttagga gacttggaag aacgtgtagc
    41461 aatgacgcca acacgtaaag ctatcgctaa agcaatggtc aacagtaagc atacagctcc
    41521 acacgtgaca ttgcatgatg aagtagaagt gtctaaacta tgggataatc ggaaacgatt
    41581 taaagaagtt gctgctgcaa atggtacgaa acttactttc ttgccatatg tcgtgaaagc
    41641 tttaacagcg actgttaaga aatacccagt attgaatgca tctatcgacg atgcaaacca
    41701 agaaatcgtt tataaacatt actacaatat cggtattgca actgatacgg atcacggact
    41761 atatgtacca aatgtgaaag atgcagaccg caaaggaatg tttgctattg cagacgaaat
    41821 caatgagaaa gcaaaattag ctcatgacgg caaactatct gcagaagata tgcgcaatgg
    41881 aacaattacg atcagcaata tcggatctgt aggtggcggt tggtttacac cagtcatcaa
    41941 ctatccagaa gtagctattt taggtgtagg aacgattgca caacaaccaa tcgtaaatgc
    42001 tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg atcaccgtat
    42061 cgtagatgga gcaactgcgc aacaagcaat gaataatatc aaacgtcttt tagctgatcc
    42121 agagctatta atgatggaag gatgataaac aaatggtagt tggagatttt gctgtagaat
    42181 tagatacagt tgtaattggt gcgggacctg gtggctatgt agctgccatc cgcgctgctg
    42241 aaatgggaca aaaagtagca atcattgaaa gagagtatat tggcggtgtt tgtttgaatg
    42301 tcggctgtat tccttcaaaa gcactgatag ctgcaggaca ccattatcaa gagtcactag
    42361 attctgaaat gtttggagta actagtgaaa acgtcaaact agattttgca aaaacacaag
    42421 aatggaaaga aaacaaagtt gtccatacgt tgacttctgg tgttggtttc ctattaaaaa
    42481 aacataaggt tgaaacaatc gaaggagaag cgttcttcgt agatgatcac acactacgcg
    42541 tcattcatcc agattcagct caaacttatt cgttcaataa cgcaatcatc gcaacaggtt
    42601 cccgtccaat cgagattcca ggatttaaat tcggaggacg tgtgttagat tctactggcg
    42661 gattagcatt gaaagaagta cctaagaaat ttgtgatcat cggtggcggt gtgattggtg
    42721 ccgaattggg cggagcgtac gcaaacttag gagcagaagt aacgattctt gaaggctcac
    42781 cacaaatttt accaacttac gaaaaagatc tagtaaaatt agtagaagat gatttcaaga
    42841 aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat aacggtgaca
    42901 gcgtgacagt gaaatatgca gtggacggga aagaagaatc cgttactgct gattatgtga
    42961 tggtcactgt aggtcgtcgt ccgaatacag atgatatggg cttagaacaa gcgggtgtcg
    43021 aagtaggaga acgaggattg atcacggtgg ataaacaagg acgtacgaac gtacctaaca
    43081 tctatgccat cggtgatatc gttccagggg cggcattagc acacaaagcc agttatgaag
    43141 caaaaatcgc tgctgaagca atctctggca aaaaagtagc tgtagattat aaagcaatgc
    43201 cggcagtagc cttcactgat cctgaattgg cttctgtagg tatgacgatc aaagaagcaa
    43261 aagatgctgg tatcgaagca aaagcatata aattcccatt ttcagggaac ggtcgggcat
    43321 tatcgcttgg taaaacagaa ggattcatcc gtctagttac gacgatcgaa gataacgtgc
    43381 ttattggcgc gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa ttagctttag
    43441 caatcgaatc aggaatgaat gctgaagata tcgccttgac gattcatcct catccatctc
    43501 ttggcgaaat cgtcatggat gcttcggaat tagcattagg tttaccaatc catatttgat
    43561 ttgaataata caggcgaaac gcaactgaaa aatcagttgc gttttgtttt ttagaattat
    43621 caataatatt attatgtaaa cttaaaaata tgacaacttc ttggtttatt accacttcct
    43681 atgctacact gaagaggaat atgaaatagg agagttgaag atgcaagaat atcagtatcc
    43741 acttgattta gattggacaa cagacgaaat ggtcattgta atgaagatgt gggaagatct
    43801 ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt ttgaagactt atcagcagtt
    43861 taaaactgtt gtcaaatcca ttggtgagga aaaacgattg ggacgggaat ttgaaaaagc
    43921 ttccggttat tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa acaaaacttt
    43981 gagaatgaac gggtgagata aatggaacag atgattgatc atatcaagtc ctggcttgaa
    44041 gaagctggtg agaaaatcaa atcttctcaa caaacattaa caatctcgca aaaatcaaat
    44101 cgaacggatc tagtaacaaa tgtcgatcga aacatccaga attttctaat cgaaaaaatc
    44161 cattcctttg atccagaggc gaaaatattg gcagaagaag aagggtgtaa tcaattatcg
    44221 gatctcgatg gaagagtctt tatcatcgat cctattgatg gcaccttgaa ttttgtgatt
    44281 gaaggggaaa atttttgtat tatgctcgct gtttatgaat ccggcgttgg aaagcttggt
    44341 ttcatttatg acgtgatgag aaatgaactt ttttggggtg gaaaaggaat aggcgtgtat
    44401 aaaaatgatg agccattgcc tagaccagca aataaatcct tatcggaagg cttacttggt
    44461 gtgaatagtt atcttttcgg tcataataaa tatggaatcc gagaaattgc ggaaagcagc
    44521 atggggacac gtatgtacgg atgcgctggt cttgaactag tggcaatctt gaaaggaaac
    44581 cacattgctt atatttcaaa ccttagccct tgggattatg cagcgggaaa tgtcttattg
    44641 gaagagtttg gtatggtaac aagcggtctt tttggtactc ctttgacatt tgaaaaaagg
    44701 gaatactatt tagcagggac ttcttgtgca tatgcagaaa tcgcagacct tttgaaaaaa
    44761 gaaggctgaa aaaaaggtag aaatcattca caaaaatgat aaaaacgagc aaaagattaa
    44821 ttttctcgaa aaaatgaaaa cattttacta tatttcatga aaaaagtctg ctataattag
    44881 cagtcgagta aaatttgccc tgtcataaag aaccaataaa aaaggagtta gttacattga
    44941 attatagaaa cgatatccgt aacgtcgcaa ttattgccca cgttgaccac ggaaaaacaa
    45001 cattagttga tgaactatta aaacaatcag atacattaga cgcacatacg caattgcaag
    45061 aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt ttagccaaaa
    45121 acacagctgt agactataaa ggtattcgcg taaacatcat ggatactcca ggacacgcgg
    45181 acttcggtgg cgaagtagaa cggatcatga aaatggttga cggcgttgtg ttggtcgtag
    45241 atgcgtatga aggaacaatg cctcaaacac gttttgtatt gaaaaaagca ctggaacaac
    45301 atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt cctgagcatg
    45361 tcgtagatga agttcttgaa ctattcatcg aattaggagc agatgatgat cagttagact
    45421 ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag cttgtcagat gatccagcag
    45481 atcaagaacc aaccatggca cctatttttg atacaattat tgaaaaaatc cctgctcctg
    45541 tcgataacag cgacgaacca ttacaattcc aagtttcatt acttgactac aacgattatg
    45601 taggtcgtat cggaatcgga cgtgtattta gaggaacaat caaagtagga gaccaagtag
    45661 cattaattaa attagacgga acagttaaga aattccgtgt aacgaaatta tttggtttct
    45721 ttggactaaa acgtctagag attcaagaag caaaagctgg agacttgatt gctgtctcag
    45781 gtatggaaga tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa gatgcgttac
    45841 ctatccttca cattgacgaa ccaacattgc aaatgacttt cttagttaat aattcaccat
    45901 ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt ttgatggcag
    45961 aacttcaaac agatgtatcc ttacgagttg aaccaacaaa ctctccagat gcatggacag
    46021 tttctggtcg tggagaattg catttgtcta tcttgattga aaacatgcgc cgtgagggat
    46081 acgaattgca agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt gtaaaatgcg
    46141 aaccgtttga acgcgtgcaa atcgatacac cagaagaata tatgggtagt gttatcgagt
    46201 ccttgagctt acgtaaaggt gaaatgcaag atatggtcca tacaggaaac ggacaaatcc
    46261 gtttgacatt cttaacacct gcccgcggtt tgattggtta ttcaactgaa ttcctttcaa
    46321 tgacacgtgg ttacggaatc atgaaccata cttttgacca atatctgcca atgcttcctg
    46381 gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat cgatacagga aaagcaacga
    46441 cttattctat catgagtatc gaagaacgcg gtacagtctt cgtagaacca ggtacagaag
    46501 tatatgaagg aatgattatc ggggaaaaca gccgtgataa tgatttgact gtcaacatca
    46561 cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa agaccaaaca tctgtcatca
    46621 aaaaaccaaa acaattgacg ttggaagaat ctttggaatt cttgaatgat gatgaatatt
    46681 gcgaagtaac accagaaagc attcgcttga gaaaacaaat tttagagaaa aatgctcgtg
    46741 aaaaagcatc taaaaagaaa aaataatctg taaaaggatg aagagagaaa ccagcttgtt
    46801 ataaaaaata gctggtttct ttttttgtcc aactcttttc tgtggagaag tcatctttgt
    46861 caatggacat aaattcagaa aatacgccga aagacgttta aaagactttt aaaaaactta
    46921 tttctagaga gtttttgttt cataataaga taatgtttca cgaaaggaga tcaacttgtc
    46981 cttcgaagaa aatcgaagta aaatggcaag agaaaggagc gggaagatga acgacttatt
    47041 aaaacaatac tttggttatg atgagtttag acctggtcaa aaagaaatta tccagaaagt
    47101 cattgatcag gaaaatgtat taggaatcat gcctaccgga agtgggaaat caatctgtta
    47161 ccagcttcca gctttactat tagatgggct tactgtggtt gtgtcccctt taatctcttt
    47221 gatgaaggat caggtggacg cggcgaatca attaggtata cccgccactt ttatcaatag
    47281 ctcgttagat ggttacgaaa cagctagacg cttccaagaa atcgatagac agcagtaccg
    47341 tttactttat atcgctcctg aacgttttat catgccggat tttatccaag caatgaagcg
    47401 atggaacgtc cgtatgatcg ctattgacga ggcacattgt atctctcagt gggggcatga
    47461 tttccgccca agttatttac agatggcgaa ccagctagat caactgccaa atcgtcctgt
    47521 aattgttgct ttgacagcta cagcgactgt gcaagtagct gctgacataa aaagactttt
    47581 aaaaattcca gaaaacaacc atattcagac tggttttgaa cgagaaaatc tacgatttca
    47641 agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga tcaacaaaaa
    47701 tcaatctggt atcatttatg cagcaacgag aaaagaagta gaccgtctct atcatttgct
    47761 taaaaaattc gatttttccg tgggtagata tcatggtggt ctaaatgaga atgagcgaac
    47821 agaaatgcaa gaagcctttt tatacgatcg cttgcagctg attgtagcga caaatgcatt
    47881 tggtatgggt ataaataaaa gcaatgtccg ttttgttatc cattatcaaa ttcctggctc
    47941 tctagaagca tactatcagg aagctggtcg ggcgggaaga gatggtcttt ccagtgaagc
    48001 catcctgctt ttctctcctc aagatatcca agtccaaaag ttttttatcc agcagtccca
    48061 aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga cagaatacgt
    48121 gtatattgag tcctgtcttc agcaatacat cctaaactat tttggcgaga caagcagtcc
    48181 gtgcaaccgt tgtggtaact gtttagatga cagagaaata gtagaagtaa cgacagaagc
    48241 acagatggtt ctttcttgct taaaacgtat gggtgagaac tatggtaaac aaatgctgat
    48301 gaaagtctta gcaggatcga aagaacaaaa gctgcaagct ttaggttttg gacatttatc
    48361 tacctatgga ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa ttgaatattt
    48421 gatatcgaat ggctatcttc tgaccatcaa tggtgagtat cctgttttga aagtaacaga
    48481 aagagggata caagtattga aaggacaaga atctgtttat cgaaaggaac ccaagaaagt
    48541 ccagcagcta tcggatgaag aaaccgatac actatttgaa gtgctgcggg aactgcgaac
    48601 ggatctagca tcagaagcag gcgttccccc gtatgtcgta ttttcagatt ccacattgaa
    48661 agaaatgagc cgaatacgtc catcaagcag attggagatg cttcagataa aaggagttgg
    48721 ccaaagcaag cttgataaat atggcgaagc ctttttatct cggataaaaa atgcggactc
    48781 taacgttctg gataagtaga aagaacgaat acgtaaaaat gaataaaaac aactggttaa
    48841 atctgttttt tttgctcctt atgaaaatca aatatgttat aattaaggaa ttagaatggg
    48901 aggtaagcac agatggattc tttgtcgaaa aaacttgcga tccaagtatt aaatgatgaa
    48961 gcagaacgga tcaaacgatt aatccgaaat caaaacaaca gtctctgtat ctcacaatgc
    49021 aaagccttcg aagaagtggt agatacacag atgtacggct tttcccggca agtcggtttt
    49081 gctgtccgta taggagtaat cggaaagaac gagggccagc tgatgttaag tgagctagaa
    49141 cgagaattga atcagttata tagtgaggtt taccaagaaa tttatgataa aaaagaagtt
    49201 ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag gcatctatta gactatagca
    49261 tcttgattcc ctatttaatt ttatgtgtat tgggattgat catggtatat agttctacgt
    49321 cgtatctatt attagaaaac ggacaaaatc cgtccgcatc agtcattaac cagagtattt
    49381 tttgggtgct tagcttgatt gctatcgcac tactttataa aatgaaaaca gatgttttga
    49441 aaaaccagcg tttgatcatg gcagccatcg cagttttgac gattttgctg ctgattgttg
    49501 tgttttttgg taaggagatc aatggggcga aaggttggct gcaaattgca ggattctcga
    49561 tccaacctgc cgaataccta aaaatcattt ctatctggta cctgtctctg actttatcta
    49621 aaagacaaaa cagtgtacaa aaagattttc ttgggactgt gaaacgtcca cttgcaatgg
    49681 ttatcggact tactgcatta gtcgctattc ttcccgattt tggtaatgcg gcagttatct
    49741 ttttgattat tttggttctg cttttagcca gcggagtcaa ttatgtgtat actttgattg
    49801 taggagtagg aggcttttgt ctaagtactt ttacgatctg gctgatcaat atcacaaacg
    49861 gaaagatctt acccggacgt ttacagtata tctataatcg atttgctatt taccaaaatc
    49921 ctttctcaga cgaattaaat aaagggcatc aactagtcaa cggctactat gccatgttca
    49981 acggaggact atttggcagg ggattaggta acagtatcca gaaaaaagga tttttacaag
    50041 aagcacaaac cgattttatt tatgcaatcg tagtggaaga actgggagtg attatgggga
    50101 ttttgatatt agcgttgcta ttctttatga ttgtgcgcat tgtccttgta ggtatccgtt
    50161 caaaagatcc gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt ttgatccaag
    50221 tatttgtcaa tcttggagga attactggcg ttattccttt gacagggatc acgttcccat
    50281 ttttaagtca aggaggctcc agtttgctga tgctttcgat ttgtgtcggc tttgtcctca
    50341 acatcagtgc ggatgaaaaa cgcaagagct taggtttata ataaaaaaag atgtaggagt
    50401 ggttcgatga aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg tgtatttcgt
    50461 gcttgtacag aattaggaat taaaactgtt ggaatctatg cagaagaaga tgaatattcc
    50521 gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa accgattgat
    50581 gcgtatctag atattgaagg gatcatttct atcgcgaaag aatgcggagc tgacgctatt
    50641 catccgggtt acggtctttt gtcggaaaac ttgaattttg cgcaacggtg cgaagaagaa
    50701 ggcatcattt ttgttggacc caaactgcat catttagata tttttggaga taaaatcaaa
    50761 gcaaaagctg cagctatcga agcggggatt gcttcgattc caggcacaga tggaccaatc
    50821 gcttctatag acgatgcctt ggagtttgcg aaacaatacg gttatccgat tatgatcaag
    50881 gcagctctag gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa gagtgcaaga
    50941 gagggctacg aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga tgaagtgtat
    51001 gttgaaaaat acatcgctaa tcctaagcat atcgaagtac agattctcgg ggatacgcat
    51061 ggaaacgtca tccatctttt tgaaagagat tgttctgttc agcgccgtca ccaaaaagta
    51121 gtagaagtag cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat ctgccaggca
    51181 gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga atttcttgtt
    51241 gaaggagatg atttttactt tatcgaagtc aaccctcgcg tccaagtgga acatacgatc
    51301 acagaaatga ttacagatat tgatatcgta acaacgcaac tgttgattgc tcaaggtttg
    51361 gatctgcata aagaaatcgg attgcctcag caagaaggaa ttaaattaaa tggttcagcc
    51421 attcaatgcc gaatcactac agaagacccg ttgaataact ttttaccaga cacagggaaa
    51481 atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg aaatgcttac
    51541 gcgggctatg tagtgacacc gtattttgat tcattgctag taaaagtatg tacgcatggt
    51601 gctacctttg aaacagccat tcagaaaatg gaacgttgct tgaaagaatt tagaatccgt
    51661 ggagtgaaaa caaacattcc atttatgctc aatgtcatca cacatcctga attccaatca
    51721 ggaaatgcaa aaacaacctt cattgatagt acagcaacat tatttgaatt tcctcgacta
    51781 cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa tggttttcca
    51841 ggtatcgaaa gtggcgaaaa accattttat gaagaaccac gtatgcctaa agatctaatc
    51901 acaagaactg attacgtaac ggctaagaat gtattagatg cgaagggtgc cgatgctttg
    51961 gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt tagagacgcc
    52021 catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat tgcacgtttg
    52081 actggagaag gtcttcctga actcttttca agtgagatgt gggggggagc aacatttgat
    52141 gtggcttacc gctttttgaa tgaagatcct tggcaacggt tgagaaaaat tcgttcttta
    52201 atgcctaata cgttgctgca aatgcttttc agaggttcaa atgccgttgg gtattctaat
    52261 tatcctgaca atgtattggt ggaatttgtc aaagaagcag cagcgcaagg aatcgatgta
    52321 tttcgaatct ttgatagttt gaactggact ccacaaatgg aaaaaagtat ccaagctgta
    52381 cgggatactg gtaaaatcgc agaggcagct atttgctata ctggcgatat caacgatcca
    52441 agtcgggcaa aatacaatgt tcagtattat aaagacatgg caaaggaact agaacaactg
    52501 ggtgcgcata tcattgcaat caaagatatg gctgggctgc ttaaaccgca ggcagcttat
    52561 cgtttgatta gtgaattgaa agaaacgaca gatctgccaa tccatctgca tactcatgat
    52621 accagcggga atggaatcat cacttattct gctgcttcaa aagcaggagt agacatcgtt
    52681 gacgtggcaa tgagtgcaat gagtgggaat actagtcagc ctagcatgag cagcttgtat
    52741 tatgcactgg tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc acagaaatta
    52801 aaccattatt gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg cttgaatgct
    52861 cctgaaacag aagtatatat gcatgaaatg ccaggaggac agtattcgaa tctacagcaa
    52921 caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat gtatcatact
    52981 gtcaacttga tgtttggcga tatcgttaaa gtaactccat catctaaagt agttggagat
    53041 atggcactat ttatggtcca aaatgatctt acagaagaag atatttatga aaaaggcgaa
    53101 acactaagtt tccctgaatc tgttgtgact ttcttccaag gagaactagg gcaacccgtt
    53161 ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc attgaatgaa
    53221 cgtccaggtc ttttggctga atcagtcgac ttcaacgaag taaaaaaaga attggcagaa
    53281 aaaattggct atgaaccaaa acaagacgaa gtattaagct atttgatgta tccgcaagtc
    53341 tttttggact atcaaaaagc atataatcaa tttggcgatg ttacattact ggacacacca
    53401 acatttttcc aaggtatacg tttgggtgaa acaatcaatg tacaaatcga acgcggaaaa
    53461 acgcttatca ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa tcgcgtactg
    53521 ttcttcaatt tgaatggaca aagacgtgaa atcactatca aagataattc gatcatcagt
    53581 acggtccaaa cccgattgaa agctgaaccg acaaaccgag aacaaattgg agcaacgatg
    53641 tctggttctg ttttggatgt attagtgaaa aaaggcgata acgttaaaaa aggtgatacg
    53701 ttgatgatta ctgaagcgat gaaaatggaa acggccatcg aagcacgttt tgacggagaa
    53761 gtcgcacatg tatatgtgtc ttcaggagac actatttcgt ctggtgacct tttgattgaa
    53821 gtgactgaga aataataagc gaaaggtggg gaaacatgaa aagactgggt ttgttttggc
    53881 tgattctgct attagttatt gtggcattct acatggaacc agtcttgttt ccgccaaaac
    53941 ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca aatgacaacg tggaaatacc
    54001 aggagcttaa agcaactggt ttttctactt atatcggaaa accgatcgat cagctggaaa
    54061 aggattttgg ccaagcctat gatcgattaa ctagcggatt tggttttgaa acaagatatt
    54121 atgaaaataa agctagtcat ctcacatttg aagcgaacat ccaagacagc agagtcacaa
    54181 ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc gttttattta gggatgacga
    54241 tgcaagatct tacgaaaatc actactatct atactaattt cacttttgag taccatgata
    54301 cagaagttgg aatcgaacta atggaagagg atatgaatta tcgtccgcta attgcttttg
    54361 ataacgacac ttttgctatt ttattttttg atcaaacaac aaacggccta ttttcagttg
    54421 tgtatttaga taaagatagt cttttgaaac tattgccata tcaagtgttt ggtgaaggcc
    54481 ttccacatta tcaagcagat aaatcggctg attgggaatc aatcaatcgc gttaaagaac
    54541 gaaaaagtac gactttatta aatatgctta gaagagaaga cgaacttccc atatacaatc
    54601 aaacacttgc ctttcaaaac cacagtcagc tgctactaca tgattttttg acaaggcctg
    54661 aggaaatatt gtcagaggaa cgtatggctg attggcaaaa atcgctaggc agtgctcaaa
    54721 cgacagtgaa attctcactg acgaatgatg aattggagaa gttaatcagt aaacaaaaat
    54781 tagaaaatac tagtgggcta tttattcatc cggcgattga cgctactttt tcgtttttat
    54841 attggtacag cgatccttac aaccatgacc gtttcatgga cccgggaacg gattctgtag
    54901 gcattgcttt ttctaaagaa aatatgttag tcttattgca agaagaaaac ggagaaagta
    54961 gtgatagcag tgatcgttaa cgaagagctg tttcaactgg aggatcaatg cctccaagtc
    55021 gctgaaatga taaaaaagag taaaacgatg gcttcttatc ttgaacataa acagtcaatg
    55081 gaagaatctg caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa agaagcattt
    55141 gaaaatgtag cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa acgagctgtt
    55201 cgaaaagcaa aaagacagct tgatatggat gagcatgttg cagctttccg atatagtgaa
    55261 acacagctgc aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga agtttctaat
    55321 gaaatcaaag tagatgcagg tagcccgttt tttgaaacga aaaaacactt tggatgcgga
    55381 ggtaattgtc atgcaattag acaatagtga atttgaaata acaaaacgac gcgggctgat
    55441 cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa aagttcggct tgttgcatta
    55501 tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat gaagagacat tggaagaaac
    55561 agaagaacga atccgtaaat tacattttgt tcgaaacgta gaacgttctt accgaccgga
    55621 tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca gtattaaaag acgaaggttt
    55681 cgaaattgaa gagctaaata cagaaatccg attagctgaa catctttaaa aatctcttat
    55741 gagcagtttt taaataaaaa ataacttgga taacgatcga tttttgatca ttgtccaagt
    55801 atttttttgg tatactagta ccagtaccat ttctaaggag tgagtagatg cggataatat
    55861 caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc agaccaacta
    55921 cagataaagt aaaagaatcc atattcaata tgattggacc atattttgat ggagagacag
    55981 tattggatct gtttgcagga agcggtggtt tagctatcga agcagtatct agaggctgca
    56041 gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa gaaaacatcg
    56101 aaattacgaa agaaccagag aaatttacca caataaaact tgatgcggac aaagcaatcg
    56161 aacggctagc agcggagaaa caacaattcg actacttgtt tcttgatccg ccatatgcaa
    56221 aacaaaaaat aatcgatcaa atccataaaa tggaagaact gggacttttt tctaaaaccg
    56281 cagttatcgt atgcgaaacg gataaaacag tcgaattgcc agaaactgtg gctggattcc
    56341 atcaaataag aaaacagatt tatggcataa cggccgtaac tatctataga aaagagggaa
    56401 atcaatgaaa cgtgcattat tcccaggaag ttttgatcct tttacaaaag gtcacttaga
    56461 tacggtggaa cgtgcggcta aattattcga tgaggtagta attggtgttt ttatcaatac
    56521 ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca ttgatcacaa aagctgtttc
    56581 tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag aaacagcaaa
    56641 agagattggt gcagatgcgt tgatcagagg catacgaagt atcaaagact ttgaatatga
    56701 acgagagatt gctcagatga atcatcatct atatgatgaa ttggaaactg tcttcttatt
    56761 agcaaaacca gaatatagtc acgtgagttc cagtatttta aaagaagtac tgcactttgg
    56821 cggagatgtg agcagctact taccgccagt gatcaacgaa gctttagctg gaaagcgtga
    56881 atcaaatgaa agctaaacaa tggttgaaaa cagtactgat tatcatattt ggaatgttga
    56941 tcattggatt tgtagtagtt cccttgccgt attatgtaga atcaccggga gccacgatcg
    57001 atctgaaaga actaattacg gtcaatgata aggaagataa aggttctggg tctttctctc
    57061 ttacctctgt tggtatcagg caagctacag gtttcttagc tttaaaagct aaaatgaccc
    57121 cttttgaaga attgatcagt gaagaagaat tgactggtgg ggcgacaaat gaagaatata
    57181 atcaaatgca acagtattat atggattctt cccaaaatgc agcaatcgaa caagcgttaa
    57241 aacttgcaga caagccttat aaaatggaat tcaaaggcgt atatgtcatg agtgtagaga
    57301 aaacatcgaa tttctttggc aagctttcag ttggagatac agtaacaaaa gtagacggga
    57361 aaagctttca aagtacagaa gcatttatgg attatgtaaa aagccaaaaa gttggacaaa
    57421 caatcgaaat cagttatttg cataatggtg aagaaaaaac agcttctggt gatctgatcg
    57481 agttaccaac agataaaaag gcaggaatag gtatcacgct tactgaccat accgagatcg
    57541 aatcagatac agatgttcgt attaattctg gctctatcgg tgggccatca gcgggattga
    57601 tgttcacttt agaaatctat gaacaagtca ctggtaaaaa cttgcgacat ggtaagcaaa
    57661 ttgcaggaac tggaacgatc aacagtgaag gagaagttgg ccgaatcgga ggaatcgata
    57721 aaaaagtggc aagtgcggat aaagctggcg tagagatttt ctttgcacca gatgatacga
    57781 tttcagacga tgttaagaaa gaatatcctg aaataaaaac caactacgaa gaagcaaaag
    57841 ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca gtacaagaag
    57901 cgcttgacta tctagagaaa atgaaatgaa actaaataat taaagagggg ttgtgacacg
    57961 agttctgtca caacccctct ttttcgtcaa aaatcttcac gataaaaata aaaacaaaag
    58021 aaaaaattga cgtaattaga tgttgaaggg acgattttgc cagagctcac tgtttcttca
    58081 attttaaatg aaaggagtaa tgaatgattt ttactggtca ttctttgaaa aaagaacaat
    58141 gatagaaaaa atcagcaaga aaatactagc ttcacccaaa ttgatcattt ggatgtctat
    58201 gatagttctt atcttttgta tcgcaagtgg ttttttatta tcaaatttgc tattttatag
    58261 agaaacagaa gagactgctg caaaagaaat actttcttcg tttagttcca gtggagaaga
    58321 tccatcagct gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca tgtacaccga
    58381 tatcaaagga agtgtgaagg agcctggtat ctattccttt tcttcagaag aacgggtcta
    58441 tgatgtacta aagcgtgcag gtggattatt agaagaagcg gatagcgatc gaatcaactt
    58501 ttctgcgaag atagaagatc agcaagtcct atatatccct gctgtaggtg aagagctgcc
    58561 agaacatctt gatcagttgg catcctcgga aggaaaacag cctacagcag attccgagcc
    58621 tcccaaaata aatatcaata cagccactcc atcagaactt cagcagattc ctgggatcgg
    58681 atcagtcaaa gcgcaagaaa tcattcgatt tagagaagaa aacggatcct tccagaaagt
    58741 ggaagacctg caggagattt ctggtatcgg tgaaaagaca gttgaaaaac taaaaaactt
    58801 cgttacaatt aaatagaatg attcaaaatc gactggcacg agcaataaat ggacgctaat
    58861 ggatactaag aataatccaa ttctaagagc ttggcaggac gaggaggtag tatttttgga
    58921 taggaaatag cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg aatgtctatc
    58981 tccaaaataa aatatctggg attcatatta tgaatacggc tgtcagcggc aataacagtc
    59041 aagctctacg agaacgcttt gccaaagatg tacttagcta tacagcagat aaagtattta
    59101 ttctcattgg gacgaatgat ctagcagagc ataaacaact ttctaaagaa acgtaccaaa
    59161 aaatctgcag tggatgatag ttcagctgaa aaaagactat agttctgatc atgctttctt
    59221 tatcacgcca ccagcagtcg atgagacaaa acaaaaatat cggaataaca agttgatcaa
    59281 agaatatatc tctgttctca cagaagtagc aaaacgccat gattgtgaag tatcgatttt
    59341 tatacggaat ttcttgaaca aatggattcg aagaattttt ataatgggct aatggatgac
    59401 ggacttcatt tcggcagtga agggtaggat cttttgtcag acaggatgat tcgttatatt
    59461 taaagaatat tttgtttaag caatgagtac tttttaatca acttgaaaaa acggaggaat
    59521 ataaaacatg ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa aagttactga
    59581 taagtcacat aatgaacgca ttccttggga ccagtacttt atggctcagg cagtactgtt
    59641 atctttacgt agtacgtgta cacgtttaga agtaggagcg acactcgttc gagaaaaaag
    59701 gatcattgct ggcggctata atggagcagt cagtggagat gtccactgca tagatgaagg
    59761 gtgttacgtc gtggatggac actgcttgcg aacgatccat gcagagatga atgcactcct
    59821 acagtgtgca aaacttggta tcccgacgga aggttcagaa atctatgtca cacattttcc
    59881 atgtttggcc tgtacaaagg ctttgttgca agctggtgtg aaagggatca attatctcca
    59941 tgactatcga aatgatcctt atgcccaagc gttgatcgaa caattaggtg tatctgtaca
    60001 ccaagttgct ttagactcac agcatttttc aaaacttcaa aatgaattaa caggaaaaca
    60061 tgtctagcgt tttagaaagg catgttcgaa atcggttgat ttttccggca attacgtcga
    60121 tgattatctg tttgttgtat tacactgaca attggggtta tcgtttgctt cttgccggaa
    60181 tttttttatg gttttcgcta aaagaaggac gttttatcgc gtacttgtgt attttgtgta
    60241 gcataacggt atccatttct tataggctac aagagtattc atttttagag gaaaaaacaa
    60301 catctgcgca aattactgta tttacagata cgattcgtgc aaatggtaat tttttgacaa
    60361 tggaaggaag gttatcagag aataaacaaa agctccagct ttcttatcaa gtaaacacag
    60421 aagcagaatt gaaaacatgg cttaacttcc gaggaaaggg aatagaattg actgctgaag
    60481 gcgagttcat ctctccagca aaaaagcgga atctttataa ttttgaccaa gaagcttact
    60541 tcaggaacca tagaatttct ggacggttcc aaattcaaaa gattgaacag atcaccatgc
    60601 aaaatcactg gcgaaactgg cttcagagaa aaagaggatc ttttatctcc tttgtccaat
    60661 ctgaatttcc gcaaaaaaca agtgtatata ttaattcttt attatggggc tataaagatg
    60721 caggattcag agaaccagaa gaaattttta gggagagtgg tttgctgcat ctgttcagtc
    60781 tatcgggtct acacatccaa ttttatttag ggtggctgta ttatttattt cgacgtatcg
    60841 gatggccact acacgtcagt attattccgt tgagtctact aactatctgt tatgtttcta
    60901 ttgcaggaag ttctatttct gttttgcgtg ctagtttgct gtttttattg cgattgctag
    60961 tcaaattgct gaataagcat tattccacca tggacttatt tgcagttgtc ttttggcttc
    61021 ttttactatt tgatcctcga tttctttttc atgcgggtgg acagcttagt tttttcatga
    61081 gttttttgtt tatcctgatt tcttttgatg gagattggtt gaaaaaaacg gcctctcacg
    61141 tcctgtttac tatgatcacg atgccgttga ttgtatggca tttttatgag tggcctattt
    61201 taggtagtct ttttacatta ctgattgcac cggtattcaa gtttcttttt cttcctggtg
    61261 tgttatttct cacactcttc aatcgctggc tgccggaaac tttacttttg ctgatagaag
    61321 agggattaat cttatttgag cgaataatcg atttttcttc agggtcgtct ctcattatag
    61381 gacgtttgcc ccttgtgcta tctgggatgt atattttcgg attactgatt tgtatggata
    61441 ggttgaaaga acaacctgtg aaacacttgt tttatgttgg tcttttttct tttatttttt
    61501 tgactttgcc atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc ggtcaaggag
    61561 acagtatctt tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat actggaggtc
    61621 agttacactt tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct gctgaaagaa
    61681 atatcgttcc atatctaaaa ggtcagggga taagtgtaat cgataaactc atcttgacac
    61741 acgacgatac ggatcatgtg ggagaattac ccacgttatc tcagcatttt actatcaaaa
    61801 aaatttatat cggttggggc gctggacgaa atgagccatt acaaaggcat ttatctgaag
    61861 ctcaaaaaaa tggtactgag atcattgaaa tcaaacatgg ggacaggatc agcggttatt
    61921 atgatcttta tgtattgact ccttttgaaa agggagaggg aagaaatgaa gattcgattg
    61981 gtctatggat ggaatataac aataggcgct ttttattttt aggagatttg aatcaagcaa
    62041 tggaaaaaca attgcttttg atttatccta atctgaaagc cgatgtagtc aaattaggtc
    62101 atcacggtag ccgtacgtca agtaacacag attttctttc ccatattgaa gcaaaacatg
    62161 ggattatatc ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa gtattggaga
    62221 cactagaaga aaatcagata aaaacatggc gaacggatca acaaggaatg atcacctatc
    62281 gctggcatcc agtttttcat cctcatgggc tagtggaaac aatgatagac tagtaattct
    62341 catctgctca tggtaggata atttttgaaa gtggtgagta atttgagtat acaagcagag
    62401 ttacaaaaaa tacgtaaaga aaaacttgca tcttgttact tgattttagg aacagaaaag
    62461 tttctccaag atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga gggcgaagat
    62521 gacttaaatt ttttaagttt tgacatggaa aatgcaagtt tagatgaggt agtagctgaa
    62581 gcagagacgt tgccgttttt cggagatcaa cggttagtat tcgtagagaa tccttatttt
    62641 ttaacaggtg aaaaagtaaa caatggaatc gaacaaaata ctgatttatt gacggcttat
    62701 ttgaaagaac cattagaatc gactgtatta gtattctttg cgccatatga aaagttggat
    62761 gaacggaaaa aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa tgtccaacag
    62821 ttgaatgaaa aagaagtacg tcaatatctg atgaatacct tagagaattc ctcaatcaaa
    62881 atggaacgaa gtgcaattga tttattcttg aggttgaccg acttagattt atccaaaatg
    62941 atgagagagt tacagaaatt ggtcctttat ggacaaaatc aacagcaaat cacggtaaga
    63001 gaagtagaag aattagttcc taaaacgtta gaacataata tttttgacat gacccaatat
    63061 attttaaaag ggaagacgga acaagctttg cgtttatacg aagatttagt tatgcaaggg
    63121 gaagaaacaa tcaaaattaa tgcgattttg ctttctcaat tacgtttatt tttacagaca
    63181 aaatttcttg taaagattgg ttatcagcag gcaaatattg ctgaaacatt gaaaatccat
    63241 ccatatcgtg taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca tttgctggta
    63301 cagttgtttg atcaattagt tgagatggat tatcaaatca aaacgggaca aatcgaaaaa
    63361 gaattaagtt ttcaactatt cgttttgcgc acagggcagt tgttaagaaa tcgataagaa
    63421 gaaagacgag ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt ttgacaaata
    63481 aaaaaatcgg ccgaagccga ttcttttatt tagcgatttt tttgcttaaa cgagatttgt
    63541 cgcggttagc tttgtttttg tggattagac cttttgattc agccatatca atagctttaa
    63601 cagcttcttt atataacgca tctacgttgt cagcacctga agctacagca tcttcaaatt
    63661 ttttgattgc agtacgcata gcgcttgttt gagatgaatt tttcacatta gcgttttcgc
    63721 ttgtacgaac acgtttgatt gcagattcaa tgtttggcat tagtttcacc tccgataact
    63781 taagttctta cgcgtgttac cgcataattc aacattcatt attataccta aaggcagcgt
    63841 gctttgcaat atgaacatga caagtttttt tccttaacag tcttttatta ggattttttt
    63901 tcagtaaaaa cggatagtac aagctgaaaa aaatcaaaaa gacgctattg tgggaggcaa
    63961 tagcgtcaaa ccaaactaga tttttattta tctatttaaa caaagtttga atgggaatgt
    64021 ttggtactaa gttattatat aacaaagaga accgtttgca aagttttttt tgaaaaggga
    64081 tacgattccc tgttgttctt tgtttttcta ttaagaaact gcagttattt tataaccagt
    64141 atcctttgat cagtaaaggc tattctggtg taccgattat tttaatgtat tcatcgatat
    64201 tttttatcaa catttttttt atgaccaact ggttccgacg gttccatttg ccacttactt
    64261 ccagcacata tttatttagg ggcaaacgta agatatattt tgccaaatag cgatctttaa
    64321 gcaggccatt gatcacacgg tcctcaagct gcaagttgat cccagtcagc atcattggct
    64381 gttcttgtaa aacgtacgta tcaattaaaa gaccttcaaa agtttccaca agcgtcttcc
    64441 tttcttttca aagtaacctg aaaacaggca ctaattcttc cattttgata ttcgttgaag
    64501 tggatgcgat cattctttct tttgtccact gtaatctagt ataacaaaaa agagcagaag
    64561 agccaaaaag aaaaaagagc atcgaattct ataggaagtc tcattggcac ggcctaaatt
    64621 attacaaaac attgattaaa aaaagcccct gcatcgcagg agcatcccct atatcgtttt
    64681 aataggtcaa tcaaaaagca gtatcaaaca cattttgtat caaatcagga aataatagat
    64741 aagctaagcc cagaatcaaa agtaccgcta gaagaaacca gccgattgtt cctatgaaat
    64801 actttaattt ttcgaacatt gtatcacctc gttgtcagta tatcacggat cagtaaattg
    64861 ggggtgttaa gaatctccta aaatagacaa aatttgttct attttcctaa agcaaatact
    64921 tgtaagatac agtaagtgat accagaagca atgaccggtc cggcggcgat tcctcttaag
    64981 aagacaacac ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg atcagaagca
    65041 atcaatccta cacccttgga agataagaca gccactagcc caccgcaaaa aacagctacc
    65101 cagccaatag gggatttaaa ggcttctaat agatcacgta gaccgatttc tcctgttgcg
    65161 atagggatta gaatagccgc tgtgatgatc gtcaccccaa tattgattcc tttgccttga
    65221 agccaaggga aaaaggaacg ggaggctggt atgagcttca taatcaaaac aaaagctgcc
    65281 gctatcatca gactttgatt tttagctaaa attgctacaa gtaaaataat tcctaaaaaa
    65341 agccaacttt ccatgaaata tattcctcac tttcttcgaa acaatcataa catgttttta
    65401 gaaagttaca aaagtaggaa ttcatccttc aagacctatt cataagctgt ttttttcata
    65461 aaataaacaa aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt
    65521 atagtggaca agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta
    65581 aacgcaagcc attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag
    65641 gtagatttaa aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa
    65701 aaataatatt ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa
    65761 atttatttaa gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc
    65821 cttgccccat agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat
    65881 caccaaccat cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt
    65941 cttccggagt acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg
    66001 ctgtgccaga atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg
    66061 aaattacttg ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact
    66121 gctgatcttt agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt
    66181 ccgcaatctc tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc
    66241 attgcgcacc taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt
    66301 attggatttc ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat
    66361 gcgtcgtttt ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga
    66421 aaaagctgtc agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa
    66481 tcgcttttaa ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt
    66541 ttgcagaatt tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag
    66601 gagaagccac aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta
    66661 ggaaaaagtg ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat
    66721 tattttcgat tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta
    66781 ctaattggat aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga
    66841 tgggatttcc ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag
    66901 gcacagattc accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc
    66961 catctatcgg aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag
    67021 aaacatctac ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa
    67081 gattcatcaa agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac
    67141 cgcttaaaca aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg
    67201 caccaatagc agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt
    67261 ctttcaagcc ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa
    67321 gtggggtgat ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc
    67381 agccgattgt tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc
    67441 aaaacaagga tagcataata aaaatgtaat tgataataat aacagctaaa tgagaacgat
    67501 aatggttatc atttggtgat taagcttgat gttgttttta ttatttataa tgattattat
    67561 taaagaaaat aaaaaaccag aacatttctc aaataaga
//
LOCUS       DACECJ010000006        61587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.006, whole genome shotgun sequence.
ACCESSION   DACECJ010000006 DACECJ010000000
VERSION     DACECJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.006"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2307)
                     /gene="esp"
                     /locus_tag="IWO09_000361"
     CDS             complement(<1..2307)
                     /gene="esp"
                     /locus_tag="IWO09_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0262983.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYH"
     gene            complement(2924..3358)
                     /locus_tag="IWO09_000362"
     CDS             complement(2924..3358)
                     /locus_tag="IWO09_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262984.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3375..4580)
                     /locus_tag="IWO09_000363"
     CDS             complement(3375..4580)
                     /locus_tag="IWO09_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0262985.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5274..5660
                     /locus_tag="IWO09_000364"
     CDS             5274..5660
                     /locus_tag="IWO09_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0262986.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5754..5945
                     /locus_tag="IWO09_000365"
     CDS             5754..5945
                     /locus_tag="IWO09_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0262987.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6459..6791
                     /locus_tag="IWO09_000366"
     CDS             6459..6791
                     /locus_tag="IWO09_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0262988.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7087..7242
                     /locus_tag="IWO09_000367"
     CDS             7087..7242
                     /locus_tag="IWO09_000367"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0262989.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7518..8858
                     /locus_tag="IWO09_000368"
                     /pseudo
     CDS             7518..8858
                     /locus_tag="IWO09_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9099..10715)
                     /locus_tag="IWO09_000369"
     CDS             complement(9099..10715)
                     /locus_tag="IWO09_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0262990.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10833..11051
                     /locus_tag="IWO09_000370"
     CDS             10833..11051
                     /locus_tag="IWO09_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0262991.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11246..11707
                     /locus_tag="IWO09_000371"
     CDS             11246..11707
                     /locus_tag="IWO09_000371"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0262992.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11900..12139)
                     /locus_tag="IWO09_000372"
     CDS             complement(11900..12139)
                     /locus_tag="IWO09_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262993.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12163..13686)
                     /locus_tag="IWO09_000373"
     CDS             complement(12163..13686)
                     /locus_tag="IWO09_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0262994.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13704..13826)
                     /locus_tag="IWO09_000374"
     CDS             complement(13704..13826)
                     /locus_tag="IWO09_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0262995.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13856..14839)
                     /locus_tag="IWO09_000375"
     CDS             complement(13856..14839)
                     /locus_tag="IWO09_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0262996.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14863..15012)
                     /gene="rpmG"
                     /locus_tag="IWO09_000376"
     CDS             complement(14863..15012)
                     /gene="rpmG"
                     /locus_tag="IWO09_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0262997.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15033..15410)
                     /locus_tag="IWO09_000377"
     CDS             complement(15033..15410)
                     /locus_tag="IWO09_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0262998.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15442..15621)
                     /gene="rpmF"
                     /locus_tag="IWO09_000378"
     CDS             complement(15442..15621)
                     /gene="rpmF"
                     /locus_tag="IWO09_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0262999.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15636..15905)
                     /gene="rpsN"
                     /locus_tag="IWO09_000379"
     CDS             complement(15636..15905)
                     /gene="rpsN"
                     /locus_tag="IWO09_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0263000.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16428..18200)
                     /locus_tag="IWO09_000380"
     CDS             complement(16428..18200)
                     /locus_tag="IWO09_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263001.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18154..19908)
                     /locus_tag="IWO09_000381"
     CDS             complement(18154..19908)
                     /locus_tag="IWO09_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263002.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20018..20587)
                     /locus_tag="IWO09_000382"
     CDS             complement(20018..20587)
                     /locus_tag="IWO09_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0263003.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20605..22029)
                     /locus_tag="IWO09_000383"
     CDS             complement(20605..22029)
                     /locus_tag="IWO09_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0263004.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22031..22699)
                     /locus_tag="IWO09_000384"
     CDS             complement(22031..22699)
                     /locus_tag="IWO09_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0263005.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22829..23413)
                     /locus_tag="IWO09_000385"
     CDS             complement(22829..23413)
                     /locus_tag="IWO09_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0263006.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23744..23977)
                     /locus_tag="IWO09_000386"
     CDS             complement(23744..23977)
                     /locus_tag="IWO09_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0263007.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(23992..24942)
                     /locus_tag="IWO09_000387"
     CDS             complement(23992..24942)
                     /locus_tag="IWO09_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0263008.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24939..25781)
                     /locus_tag="IWO09_000388"
     CDS             complement(24939..25781)
                     /locus_tag="IWO09_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0263009.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25783..26502)
                     /locus_tag="IWO09_000389"
     CDS             complement(25783..26502)
                     /locus_tag="IWO09_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263010.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27194..27352
                     /locus_tag="IWO09_000390"
     CDS             27194..27352
                     /locus_tag="IWO09_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0263011.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27424..28650
                     /locus_tag="IWO09_000391"
     CDS             27424..28650
                     /locus_tag="IWO09_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0263012.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28738..29130)
                     /gene="rpsI"
                     /locus_tag="IWO09_000392"
     CDS             complement(28738..29130)
                     /gene="rpsI"
                     /locus_tag="IWO09_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0263013.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29146..29589)
                     /gene="rplM"
                     /locus_tag="IWO09_000393"
     CDS             complement(29146..29589)
                     /gene="rplM"
                     /locus_tag="IWO09_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0263014.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30108..30575)
                     /locus_tag="IWO09_000394"
     CDS             complement(30108..30575)
                     /locus_tag="IWO09_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0263015.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30695..32233)
                     /locus_tag="IWO09_000395"
     CDS             complement(30695..32233)
                     /locus_tag="IWO09_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0263016.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32313..32999
                     /locus_tag="IWO09_000396"
     CDS             32313..32999
                     /locus_tag="IWO09_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0263017.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33200..33727)
                     /gene="msrA"
                     /locus_tag="IWO09_000397"
     CDS             complement(33200..33727)
                     /gene="msrA"
                     /locus_tag="IWO09_000397"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0263018.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33811..34488)
                     /gene="pgmB"
                     /locus_tag="IWO09_000398"
     CDS             complement(33811..34488)
                     /gene="pgmB"
                     /locus_tag="IWO09_000398"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0263019.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34485..35615)
                     /locus_tag="IWO09_000399"
     CDS             complement(34485..35615)
                     /locus_tag="IWO09_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0263020.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35615..37798)
                     /locus_tag="IWO09_000400"
     CDS             complement(35615..37798)
                     /locus_tag="IWO09_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0263021.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37795..38820)
                     /locus_tag="IWO09_000401"
     CDS             complement(37795..38820)
                     /locus_tag="IWO09_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0263022.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38865..39692)
                     /locus_tag="IWO09_000402"
     CDS             complement(38865..39692)
                     /locus_tag="IWO09_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0263023.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39689..40756)
                     /locus_tag="IWO09_000403"
     CDS             complement(39689..40756)
                     /locus_tag="IWO09_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0263024.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40782..41618)
                     /locus_tag="IWO09_000404"
     CDS             complement(40782..41618)
                     /locus_tag="IWO09_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0263025.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41615..42484)
                     /locus_tag="IWO09_000405"
     CDS             complement(41615..42484)
                     /locus_tag="IWO09_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0263026.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42554..43837)
                     /locus_tag="IWO09_000406"
     CDS             complement(42554..43837)
                     /locus_tag="IWO09_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0263027.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43851..45536)
                     /locus_tag="IWO09_000407"
     CDS             complement(43851..45536)
                     /locus_tag="IWO09_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0263028.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45811..46836
                     /locus_tag="IWO09_000408"
     CDS             45811..46836
                     /locus_tag="IWO09_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0263029.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47431..51084)
                     /gene="rpoC"
                     /locus_tag="IWO09_000409"
     CDS             complement(47431..51084)
                     /gene="rpoC"
                     /locus_tag="IWO09_000409"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0263030.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(51163..54780)
                     /gene="rpoB"
                     /locus_tag="IWO09_000410"
     CDS             complement(51163..54780)
                     /gene="rpoB"
                     /locus_tag="IWO09_000410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0263031.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55508..56476
                     /locus_tag="IWO09_000411"
     CDS             55508..56476
                     /locus_tag="IWO09_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0263032.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56494..57399
                     /locus_tag="IWO09_000412"
     CDS             56494..57399
                     /locus_tag="IWO09_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263033.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57396..58202
                     /locus_tag="IWO09_000413"
     CDS             57396..58202
                     /locus_tag="IWO09_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0263034.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58359..59327
                     /locus_tag="IWO09_000414"
     CDS             58359..59327
                     /locus_tag="IWO09_000414"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0263035.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59644..60168)
                     /locus_tag="IWO09_000415"
     CDS             complement(59644..60168)
                     /locus_tag="IWO09_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0263036.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60455..>61587
                     /gene="scm"
                     /locus_tag="IWO09_000416"
     CDS             60455..>61587
                     /gene="scm"
                     /locus_tag="IWO09_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0263037.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASS"
BASE COUNT        18692 a        12363 c        10311 g        20221 t
ORIGIN      
        1 gtggtagcct gcaggaatgt tttctcctgg attttcataa atatctttac ttacagttac
       61 tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca attttaatag gaattgttcc
      121 agtaatcggc tccgattctc ctacaccttt agctggagtg aatgtaacaa taccagtgtt
      181 tgcatcaaca cttgcattac cgaagtactt attatagaca tctgaaattg aaaaggcatc
      241 tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt ccactttgaa ctgtcgtttc
      301 tggagttgtt gttataggta cgtatgttgc atcattttcc atagcttgag aagcttttac
      361 agcaaactct acatccacgt cgaaagttcg atttccgatc ttaacagtta caataccttt
      421 cgtactatca gtaacgttag aagtatcagg ctctgttttc catgaatact ctgcatcttc
      481 aggtaagttt ttattattcc gaattgattc tttagcatct ggtttaaatc caactaccac
      541 ggtttgttta tcaaccgctt ttggtgattc cttaataacg gttgaacctt cttctggttt
      601 atcaaaacct ggagaaacga tttggatttt taattcatct ttcgcgatta atttacttga
      661 atctacaccc gtaaattcaa attctacagt atctgaaatt ggagccccat ctttttcatt
      721 tggagcgata gttttttctg ctaatacagt accttcttta tcaacaagtt gggctttgtg
      781 acctgttccc gctaactcgt ggatgaatac tttcccctta actgttgtgt caacatcata
      841 tacttcttct aattcaggtt tcccgatata tggattcgtg tctccgctct cttctttatc
      901 gtcagggttt ccatcaccat caatatcttg caaataataa taacctgttc cataagtgtt
      961 ctggattaat tttttattcg tatccgacaa ccaagaatca aagatgaaat cttcaccaaa
     1021 tggtttattt tcttcaactt gtgctgttgc tttcatatct tctaagattt catctaatgg
     1081 cttagtcaat tgtaaaacat atcgaataac gactggacgt ggacttgcat tagcaaaatc
     1141 gataagttta gttaaatcat tattagttat tgaacctatg ccataatttt catgaccttc
     1201 ccgtcgctcg attgctaaat cagctacttt tgttccggat tcaaacgaaa catcatatgt
     1261 tactttgtca gcctgaacca tgtgcatctg tatacccgct acatatggaa ctaatcgttc
     1321 atcaattttg taattagcat accaaggcgt gccctttgcg gaagatgaat atgagaaatt
     1381 ataatttttc gttaacttat gatctaagat tatagctcca ttagcacctg tggatcctga
     1441 accatctttt gtttgagtat taggtttctc atattttgca cttactgatc catgtttaaa
     1501 ctggtcagga ttattttcag atacatctaa gctattatag aaatcatctg aatctgttaa
     1561 aaagtagccc gtactatcaa tacccggtac aattttatca tcgtcttgat gacttaccaa
     1621 gtagattcga tacattaaac gatcggtctt gagattgtct atgcctatct ctttaaaaat
     1681 ttcatctagg ctcttttcaa acgtaattac cccattagga gcggtttgcg tatcggttgt
     1741 ttctgcccca gcaaataatc catcacttgc acgaatataa ttgaccttcc aaatatttgt
     1801 aggtctaccc agcgaatcat taattcttac aaaggtccgt ctagaagatc caattccttt
     1861 ctttggatcg acctgaattt tttctacata tttagctaat cgttcatcta attgaagacg
     1921 gatttcccag tttttagttg atgctgcaat attatgtttt ccgtaaactt caaatccaat
     1981 tgtattgcct tttagtgtgt ctccagttag ttcttgagga ctaaattgta aatccgaaaa
     2041 gttataattt ttcgtataac caggattctc ctgaaaagta ttccccgaag ctactttgtc
     2101 acctacttgt acggagctta tcgctgtttc aagtgggctg cttggagaaa tttcatcttg
     2161 tgctttaact agttcagttg ttcccaagac aagaccaaca ccaatcaaaa cagatgcaac
     2221 cccaacactt aattttttga tactgtactt caatactcgt ttctttgttt tttctagaaa
     2281 tactctctta ttattcttgc taaccattat tcaaatcaac tcccttaata attactttcc
     2341 tagtaagaat aaaagataaa tctgatacgt attattcaca gaataggaat cttactaacc
     2401 ttatgttaac taatttactt agagaatttc atgaacatta caacaaaaaa agtacatttc
     2461 ttcatgtttt tttcggatat ttgtgctttt taaccattca gcagctgaaa acatataaaa
     2521 agatatgtat gtattctttc tgaaattaag ttcatttcta tgcgatgaag catgttttca
     2581 aaatattacc ctttcttaga ttaaacttca gagtattcaa ataacggaat aaggagcctt
     2641 atcccgcatt actgaatgag actgtgacat aagtaaaaag gtgacttcaa attccttctt
     2701 cggaatttga aatcaccttc tttataatta taaagtatcg gtttcacagc atttctgaat
     2761 gattcctaga atcttaaacg aatgattgga gttatcaaca ttttttcact cgtttttagc
     2821 gaaacaaaaa atgacatttg tcacagcctc attaaattat tgattctttt tctttataat
     2881 tgaattgtcc atatttaaag tgtctagtac tctacatgaa taattaattg ctttcactca
     2941 aatacttata atctattaac ttgtagttgt ttaacttttc tgaaaaagtt agacccacgt
     3001 aataatcttc ccagctttct ctttcaatta actgatccaa tttttgataa tccataaatt
     3061 catcatgtat caaaaagaat aaataacttg atattttagc atctagctta atcagattaa
     3121 ataatgtttg tttaaaattg ctaccattaa tatgactgat agattctcta gcttcttcac
     3181 ataaactttt tactttatta attactactt tgtcatattc atttaacaca ctattattac
     3241 cattacttag acgctcagat tctacgaaac aactcaaatc agaaatttcc caaaaaaatt
     3301 ttttgttaaa atccaatttc ctcaactcct ccaaactacg taaatgatat agtctcataa
     3361 taaaataaaa cctctcagct aatgttgttg aaattgtcca ctaaacctaa ttgatattgc
     3421 ttaggagata cacctgcgta ctttttgaat actctcgaaa agtgtgaaga atctgaaaat
     3481 cctaaaccta aagaaacttc actgatagta tggtttgatt tcaacattat tttagccgta
     3541 gaaatttttc gcttagagat ataattataa atcgtaatat gcatttcatt cttaaagctt
     3601 tctcttaatt tagtttcact tacagaaaaa taattagcta tttcctttac agtaattctt
     3661 ttatagatat ttaagccgat gaattgaacg acagaagaaa ttaagggaga aagatgctct
     3721 atcttagttt ttccgatttc cttagtataa taaacaatcc cagaatccct cacttttaag
     3781 acctcaggta acgatacagc taattcgttt tcttgtatca aagcatctct actttgatac
     3841 gcttctatta tatccattcc taatgttata gacgtttgtg ctaacttttc aaaaacaatg
     3901 atagaataat ttttttcaga ccgaatcgta tctccactaa cacttggaat tattccatta
     3961 ctcagattaa aaaccatgtt ttctaaattt cgaatatctc catttttaac atattccagt
     4021 attttatttt cataaagaaa cagatatatt tctggatcgt aattttgtga taacagatta
     4081 tctattttga tttgctcgat agttttactt agattttctg tatattcaat taacggttca
     4141 tggaatagat cttctatttt tccagtaaaa aaataatgaa ctaaaatcag aatatctcga
     4201 atgtctccta aagaatacaa agtcaaattt tgcaatgaat tatagagtcg ttctctatca
     4261 gccattgcaa cattcaaatt gttcattttt agtttaaaaa aaactttatc aatattattt
     4321 gctctaaatg gaccaaataa gtaatatctg gttttaaaca tgtatagaag aaacatctca
     4381 ttaaaatcac cagtaaagaa atggaaatct ttagtttttt taatattatc agttagtaac
     4441 tttttataat cataaaaaaa ataagtagtt ctatctgagc gatactcttt tataatactg
     4501 aattcggaat taaaaaccac gataggtaac tttaaacaaa catgaagaga ctttaataag
     4561 acttctttat ctgaaaacac ttttgtttct ctctttcctt tctacatcat taaatagtat
     4621 tttatacgtt atctaaaata ctattcctct gtcaaaataa gctaacttac ttcattataa
     4681 aatatccact tatcgctttc atgtatattc caccgtaaaa agtacatttt atcaccgaga
     4741 actcatcatt tttcatcatg atgaatacta tttacatata tctagttcaa agacaataat
     4801 tattatgatc cgttattatc taagcactat acagataata ggaatttttc gtttctaaat
     4861 attccaattt gggaatccaa taaaaatttt cgttcaaata cgtgtctatt cgaactcttt
     4921 tctagttgtt aattatgaag ggggaagaaa taaaaaatac aatctataca cgaataagaa
     4981 tattatccaa aaggaggttt tagaaaagat ttattatacg taaaaagctc aagacatttt
     5041 cttagccata aggacatgtt tgatatttaa gaagatatat gaattttctt ttctaagtat
     5101 aaagataact atccattatc aactaaaaaa acatttttga aaataaacat cagcagtcct
     5161 atcaatgcat gtgagcttat agaagttatt cgcagcgcag cataattgca ttagcttctt
     5221 ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga ggaggtgtgc aatatgtcag
     5281 ataacgacga acgcgtaaat gcccagttga ttcgattctt tgaaaaggtc attttgaaca
     5341 ccgctaatac ttattataaa aaacaagaag aaattcaaaa acatgaacaa cttgatcaac
     5401 tcccaccata tttgtttact aaagatacta taaaatttaa acaaccgatt aatgttttaa
     5461 atgtgactct ccttgttgaa gatataaaat tagcaaaaat ccttcctttg ttgaaagaac
     5521 ctgaacaaat ttttcttatc gaaaaattta tctttgataa aactgataaa gaaattggaa
     5581 aggttctggg gattacaaga cagggtgcga caaatttaaa acatcgactt tacaaaaaaa
     5641 ttcaaaaaat gctggtttaa tatctgcata ataactaatt ttgtatgctt ctttgctcgt
     5701 tctcctcctt cccaaagaaa tagattataa gtccatggga agaaggtaga tttatgaatt
     5761 ctttaaaaga agatttcatt ttagcaaagg ctggaaatga agaagctgtt gaggcaattc
     5821 tcaaaagatt tagctctttg attcataagc aatcctggag aactggaaaa tatgatcaag
     5881 attgttatca agaatgtatg cttgcaatat atctagctat ctcgaaattc gaaattaaag
     5941 aataactcaa ctaagggagt ctgtgacata agtaaaaaga tagtttcaaa tttcgaacaa
     6001 taaatttgaa actatcttct ttataattat aaagtatcga ttttacagcg ttctataatg
     6061 attcatagaa tctaaaacga atgattttaa ttatcaacgt tttttaattc gtttttagct
     6121 taaaaataac ttttgtcaca gtctcactcc taaaattact ttatttacgt atgaagtggg
     6181 caggaggtcg aatccagaac tgggctatgg tacttcaacg aggacttctc ctctagaatt
     6241 gaaaagcatg cgatttacct tccgtaacga cggtagattt attcaactat gtgtccaatc
     6301 aaaatttgtc tgatttcaaa aatagtcaag agaattttgg ttgttaaaaa ctcttatcta
     6361 tcaacttcca cagtttagtt gacaaaccct gatactaagt tgggctatga tacacttttc
     6421 ttgactatca gttacgttac cggaaggaat gaactctatt gagaaaaaca tatgatcgta
     6481 aatttaaact aaagatttca caggatatcc ttgagaaaaa agtaactacc aagaaaattg
     6541 ctgaagaata caatatctct cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat
     6601 acgggaaaaa tgcttttgct ggacaaggaa aaagattacc tgataaagca gatttctaca
     6661 ttcttgaaca ggaaaataaa cgactaaaag aggaagacga gtgttctacg aacacaaaga
     6721 tcgatacgga tctccaggga tcactcaaaa actttacgat gaaggaatcg aaatcaataa
     6781 acgtgttgta gctgttctta tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg
     6841 caaatcttct catggtagag aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga
     6901 taatttgaac agaaccaaat tgatgctgtt tgggtgatgg atattactta cattccatgt
     6961 aatgacggtc gcctctattt gtccacctat attgacttag caactcgtat tctacgttac
     7021 tatatggtag attcacacat gaaaaaagag attgtcgtcc gtccattaca aatttattaa
     7081 ggagaattgc ctacggtaat tcattccgat agaggtagcc agcatcggtc ttttatttat
     7141 caagagctgt tgaacgacca ccacatcaca cattcagtga gccaaactgg gactcctgtt
     7201 gacaatgcag taattgaagc gtttcatcga tcaatcaaat gagaattaat cgactccaac
     7261 aaatataaaa gtaaaataga gatgaaggtg ttaatccaag attatttaga agactactac
     7321 ccaaacaaaa gaatccatac aaaatttatg atgactcctt gacaatttga agcgaagaaa
     7381 ataagggatc tataatatct agtgttggtg acgccttctg ttgccaatac tttttctttt
     7441 ttctaataaa cgcaaacaga caccttaaaa tcctttagaa taaagtcgac gaaaaccatt
     7501 caaaggagtg tttcaagatg tcctattcag agtttaccaa agagttacta gatattctag
     7561 acctaaatct tatctttcat gaagatgcct ttcgcaaaga gcgaatcaat gacgaaacat
     7621 gttttgtttt tgatggcaca ttgacctatc aaccggagga atgctttcat cgccactatc
     7681 aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt atccatctta ttgaatgatg
     7741 tcagtaacta caagaccatt ctgcggatta acaaacagcg cttcaaatgt aaacactgtg
     7801 gaaagacttt tttagcagaa gattcagtta gtgatcgtcg ctgctccatt gctcgcagag
     7861 tcaaacaagc cattcttgag ctattaagcg aacctctttc tatgtctttg atcgcacgta
     7921 tgaagcacat ttctccgacc actgtcattc gaatacttcg cagccttcgg cctaaaacca
     7981 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
     8041 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
     8101 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
     8161 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
     8221 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
     8281 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
     8341 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
     8401 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
     8461 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
     8521 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
     8581 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
     8641 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
     8701 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
     8761 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
     8821 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
     8881 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
     8941 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     9001 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     9061 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     9121 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     9181 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     9241 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     9301 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     9361 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     9421 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     9481 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     9541 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     9601 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     9661 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     9721 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     9781 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     9841 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     9901 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     9961 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
    10021 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
    10081 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
    10141 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
    10201 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
    10261 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
    10321 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
    10381 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
    10441 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
    10501 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
    10561 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
    10621 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
    10681 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
    10741 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
    10801 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
    10861 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
    10921 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
    10981 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
    11041 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
    11101 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
    11161 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
    11221 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
    11281 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
    11341 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
    11401 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
    11461 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
    11521 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
    11581 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
    11641 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
    11701 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
    11761 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
    11821 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
    11881 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
    11941 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
    12001 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
    12061 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
    12121 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
    12181 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
    12241 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
    12301 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
    12361 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
    12421 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
    12481 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
    12541 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
    12601 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
    12661 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
    12721 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
    12781 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
    12841 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
    12901 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
    12961 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
    13021 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
    13081 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
    13141 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
    13201 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
    13261 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
    13321 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
    13381 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
    13441 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
    13501 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
    13561 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
    13621 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
    13681 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
    13741 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
    13801 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
    13861 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
    13921 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
    13981 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
    14041 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
    14101 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
    14161 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
    14221 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
    14281 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
    14341 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
    14401 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
    14461 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
    14521 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
    14581 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
    14641 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
    14701 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
    14761 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
    14821 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
    14881 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
    14941 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
    15001 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
    15061 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
    15121 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
    15181 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
    15241 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
    15301 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
    15361 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
    15421 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
    15481 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
    15541 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
    15601 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
    15661 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
    15721 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
    15781 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
    15841 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
    15901 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
    15961 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
    16021 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
    16081 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
    16141 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
    16201 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
    16261 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
    16321 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
    16381 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
    16441 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
    16501 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
    16561 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
    16621 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
    16681 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
    16741 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
    16801 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
    16861 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
    16921 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
    16981 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
    17041 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
    17101 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
    17161 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
    17221 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
    17281 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
    17341 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
    17401 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
    17461 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
    17521 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
    17581 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
    17641 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
    17701 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
    17761 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
    17821 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
    17881 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
    17941 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    18001 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    18061 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    18121 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    18181 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    18241 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    18301 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    18361 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    18421 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    18481 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    18541 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    18601 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    18661 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    18721 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    18781 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    18841 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    18901 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    18961 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    19021 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    19081 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    19141 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    19201 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    19261 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    19321 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    19381 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    19441 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    19501 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    19561 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    19621 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    19681 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    19741 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    19801 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    19861 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    19921 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    19981 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    20041 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    20101 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    20161 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    20221 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    20281 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    20341 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    20401 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    20461 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    20521 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    20581 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    20641 agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat
    20701 aattctattg catgttttat aaagaaattc cggatcatga gtagctataa ctactgcacc
    20761 cttttctttt aagtctttaa taaggtcagc aatcctttcc atattaatat aatccaaccc
    20821 acttgtaggt tcatcaaata gaaataaatt agtttttgac aatgaagcta tagaaaccaa
    20881 taatctttgt ttttctccac cggataaatc aaacggtaat tgatctttca ttttataaag
    20941 ttccattttt tttaaaattt tttcttgctg atctttacta actatttcat tccccagggc
    21001 taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa
    21061 cggcgatgac aaattttttt tattttcgat tatctttcct ttggtagggc gaataatctt
    21121 catgagtatt cttagtaagg ttgttttccc cacaccgttt ttccctacca aaccaataac
    21181 atctccaact ttgacatcaa aatttatgtc ttttagaata ttttcataag taatttcttc
    21241 aacttttaat acatctgcac ttaccttttc gatataggga acttcaatcg aactattaaa
    21301 aatgtcaaat ttccttaatg cgttattgtt ttcagttttt aacaactgaa tagttgtagt
    21361 ttttacagtt ctatttttaa caaatagaac ttgatcaatt aattctttca aatagtaaaa
    21421 acgatgttca gctataacaa tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc
    21481 aagtttctta gtcgctttga agtccaaatt tgaagacggt tcatccaata taattaattt
    21541 agagtttagc gataaactac atccaataga aattttctgc cgttcaccac ttgatagcga
    21601 aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt cattgattct
    21661 tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta actcactcgt
    21721 tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa tattttttga
    21781 tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt ccccaaccaa
    21841 ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg tggatttccc
    21901 actcccagaa gaaccagtaa tcaatgtaag actgcctttt tttattgaaa aattaaggtc
    21961 aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa attcaaacaa
    22021 ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac aggatgatat
    22081 agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga gattctatcc
    22141 cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt ataagaacgg
    22201 gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga atgtttctca
    22261 tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag aatctgatca
    22321 ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa gaggataata
    22381 attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc ggcaacattc
    22441 gaaatacaat atataaggat gtcgcaaaaa atatagtaaa aaactctatc ttgatagcat
    22501 caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa atacatgtct
    22561 ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc acttgaaata
    22621 tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta atcaaggtta
    22681 acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg ttatcttaag
    22741 ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag cataactgag
    22801 aacaattctc aatgcaagct ttttttaatt aactcagtaa ttcaatccac ccaggaacag
    22861 aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag ctatgcttca
    22921 atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct tcttcattaa
    22981 agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa cagactgtct
    23041 ttttcgtcac aaatgatgta attttttcaa caatatcttg atatttggtt ccagctgatt
    23101 ggaataaaat caaatcgaat atacgtttat tctcaaaaat ataattaata aatggatcta
    23161 attcattgaa gttctctctc caacttttct ctatatctat attttctaag tttaagaggt
    23221 atttggtaaa ctcttcatta tacatattaa taagcgcatt aatatgcggc tgaataattt
    23281 cttcaaataa cgcttcctta gaagtaaaat gtttataaaa agcacccgta gttacgcctg
    23341 cggtcaggca cagttgcctt atcttagttt gctgaaatcc ttctttttca aaatggctta
    23401 gtccactttc tattaacttt tggtgagtta taatataatc tctggtttct atgggtactt
    23461 cctcctaata agatgaatta actttattat cttacacagg acttgaagtt tcaatcgtta
    23521 acgtttatta ctgaactcat tccttagagc taaactaatg agaaagagca gaataactga
    23581 tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc ttttctaaaa
    23641 taatccgaga aagatttaaa aaaattatat ccagctatta ggataagtcc tatcataatc
    23701 actccaatag ccattttctt atcctcccta aaaataaagt cttttactta aaaccttttt
    23761 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
    23821 cctcatatct aagtttagcc agcatctcac ttacagatgc tggactaaca cttagaactt
    23881 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
    23941 aataaattag tatagtagtc gttttaattt gtaccatacc taccctccaa attatttcga
    24001 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
    24061 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
    24121 ttttgatact gttttcattg gtctatcatc cacactgctt tctacgaata aggagggaac
    24181 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
    24241 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
    24301 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
    24361 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
    24421 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
    24481 accgttttct aaatttaacc aagcatgagg atcttccttc cctttctcac tctgaccctc
    24541 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
    24601 cgcattttta actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
    24661 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
    24721 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
    24781 ttcggtaata tctgctaaaa tcgaattagt aactactact tttaattttt cgttcgaagc
    24841 cactttatct ttgtctttac tatttattga acatgccccg aatataaaaa gagcaatcaa
    24901 aatccccaga cttcccagta ctgctttcca ttttctcatc ataacgatct ctccttcttt
    24961 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
    25021 aaaacaatac ttgatcctgc tgctatgtta aaactataac caataaacaa tccaagcaat
    25081 gatgaacttg ctccgactgt tgcagaaata aatatcatag ttttcaagct atttgcatat
    25141 aaatatgctg tagctgctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
    25201 tgcatagctg ttactgctac taaagttaat agaaccatca atagataatg ataaaaactg
    25261 acattcatcc ctattgcttt tgccattaat ggatcaaaag aagtgattaa taattcttta
    25321 aaaaatagaa ctactataac caacactaaa accgaaacgc caattgtaat ccacttatca
    25381 atatcttgta cagctaaaat atttccaaat aaaatatgaa ataaatccgt tgagctattt
    25441 gcaactccta taaggataac tcctagtgct aaaaaagaac tgaaagtaat accaattgct
    25501 gtatctcctt ttattacact attgcttttt ataaaagtaa taatgataga agctaataga
    25561 ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa tataagaaat tgctactcct
    25621 ggcaaaacag catgagaaat agcatcaccc ataagagaca ttcctcttaa aataataaaa
    25681 caacctactg ttccagaaac aatccctata actacagatg taattagagc attctgtaaa
    25741 aagtgaaagt catacagacc attaataaaa ttttgaagca tattattccc cctctccaat
    25801 aaatatagta tctccatagg ctttttttaa attttcttca ttaaatacag attctacact
    25861 tccgtgagca attagatttc gattcaaaag aataatatta tcaaaatact tctttacctt
    25921 gcttaaatca tgatgcacaa ttagcactgt ttttccctga tgtttcaatt tttttaacgt
    25981 atccataatt attcgttcac taactaagtc tatccctaca aatggttcat ctaaaaatat
    26041 gaaatccgca ttttgagcaa gacatcttgc taaaagtaca cgttgaaatt gtcctccaga
    26101 taattctcct atttgatgat tagaatattt tcccatatcg accttttcca aagcctttga
    26161 aactctttgc cattctgcat ttttaattct ttgaaaaact ttcattccgg catatgttcc
    26221 catagataca cattctttta cttttattgg aaaagtaaaa tcaatatcag ctttttgctc
    26281 tacatatgca atcttcacca gttttttatt gatatcttca ttattaatgg agacctctcc
    26341 ctgatgaggg ataatattta acattgcttt aagcaatgtg gattttccag caccatttgg
    26401 accaataata cctgttaaag caccttcttg tatttttaaa gaaatctcgt tcaatgctaa
    26461 aaaatcgtta taacaaacat taagcttttt cacttctaac ataactttct ccttctaaat
    26521 gataatgatt atcacgatca tatggtatac aaaaattata ttttagtcaa ccctaaattt
    26581 tatagtttag atttttttct gttgctgaag attagaatga caaaactatt tgaaactacg
    26641 cgtatagtaa gtagctttca aaatatataa aaaaggagcg ttctcaaatg agaacatgct
    26701 cctaaaacta ctttattggc gtatggaaaa attatacttg aagtggatag cgggttgaat
    26761 aagttgataa gcatcaaaac atttataaaa attattcaag actatgtctg ttccatatac
    26821 aaatatgaaa atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt
    26881 ttatcgttat taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca
    26941 atttttgaat aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt
    27001 agcgacaaat acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc
    27061 agcaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg
    27121 tgcttaccca aagcaattca aaaatttcat aactactatt ttaggagatg attttataga
    27181 atccagagtc tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac
    27241 tctagataag tggattcgta tatataattt cccagtaata agagttggtg gtgtagttcg
    27301 ctttgacgta gaagatatta aagactttat gaatacattt aaaataactt gaaattttct
    27361 tctcgccgca tatactaaaa ttagtgaaat atactgcctt acttttttta aggagtgagg
    27421 attttgccta caaagctttc aaatgggaaa tataaaacta atctgagata tccaaaaagg
    27481 tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa aaacatttcc aaataggcaa
    27541 ttggcgatta aagccgaaaa tgatatgaag aagaaaatag aaaaagtttt gagagaggag
    27601 aatgcaaact cattagaatt aaaaggaaaa ataacgttta aaaaatttta cgaatctaaa
    27661 tggcttccta gatatgaatt aggtcaaaca attagatcaa acaggcctcc tagtgatata
    27721 accattagta atacaaaaga tatatttcgg ttgcatattt tacctatgtt tggtgaatac
    27781 gcaatgaatt atctaaatat caatactgag ataatatcag atgaattaac caaaaaatcg
    27841 aaagaatatg caaatataaa aatcataaaa ggatatgttc gttccatgtt cgatattgca
    27901 gaaatactaa attatatcga atttaatcga actactaaaa ttattcaaag tatcactgca
    27961 cctaaaaaaa atgctttaga agaaaaaaga atacaagaag gtaagcaagc actatcatca
    28021 aaagaactta ctaattggat agaagcagta aatgatgatt ttaataatca tttacttact
    28081 tttcatgatt acacactttt tatgattacc ctgtatcttg gtgatagaaa aagtgagact
    28141 tacgctttgc aatggaaata tattgatttt gaaaaacaaa cggtccgtct taaacatact
    28201 ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa aagataccgt cattcaagta
    28261 ccagaagttg taatgacatt gctaagtgaa tggaaatcgg tacaagctga tcaattgttg
    28321 aaattaaaaa ttaagcaaac gttagatcag tatctcttta cttatactaa accatctgga
    28381 gaagtaaatt gtcctgttca tgccgattac ctaaactatc gaatcaattc aattaaaaga
    28441 aggcatcctg acttagttca tttatctcca cacaaactcc gtcatactta tgcaactata
    28501 gctagacaag gtggagcaga tatgaatcaa atttcaaatg ctttaacaca ctcagatatt
    28561 tctacaacaa agatatatgt gaacacacca gatatagttg ataaggctgt ttttgaagct
    28621 tttcagagag gattgaataa gtgtgattga tatgtgttct taaatgtgtt cttaaaaaca
    28681 aaagaagcac tttccaaata attgaaaagt gccagaaacg ttgatatata aaggatatta
    28741 acgttttgag aattgtgaag ctttacgtgc tttcttaaga cctggttttt tacgttcgac
    28801 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
    28861 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
    28921 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
    28981 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
    29041 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
    29101 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
    29161 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
    29221 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
    29281 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
    29341 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
    29401 cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt
    29461 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
    29521 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
    29581 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
    29641 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
    29701 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
    29761 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
    29821 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
    29881 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
    29941 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
    30001 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
    30061 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
    30121 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
    30181 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
    30241 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
    30301 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
    30361 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
    30421 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
    30481 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
    30541 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
    30601 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
    30661 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
    30721 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
    30781 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
    30841 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
    30901 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
    30961 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
    31021 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
    31081 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
    31141 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
    31201 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
    31261 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
    31321 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
    31381 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
    31441 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
    31501 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
    31561 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
    31621 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
    31681 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
    31741 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
    31801 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
    31861 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
    31921 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
    31981 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
    32041 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
    32101 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
    32161 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
    32221 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
    32281 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
    32341 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
    32401 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
    32461 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
    32521 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
    32581 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
    32641 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
    32701 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
    32761 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
    32821 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
    32881 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
    32941 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
    33001 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
    33061 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
    33121 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
    33181 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
    33241 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
    33301 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
    33361 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
    33421 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
    33481 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
    33541 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
    33601 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
    33661 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
    33721 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
    33781 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
    33841 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
    33901 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
    33961 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
    34021 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
    34081 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
    34141 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
    34201 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
    34261 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
    34321 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
    34381 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
    34441 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
    34501 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
    34561 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
    34621 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
    34681 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
    34741 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
    34801 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
    34861 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
    34921 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
    34981 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
    35041 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
    35101 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
    35161 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
    35221 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
    35281 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
    35341 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
    35401 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
    35461 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
    35521 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
    35581 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
    35641 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
    35701 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
    35761 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
    35821 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
    35881 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
    35941 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
    36001 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
    36061 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
    36121 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
    36181 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
    36241 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
    36301 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
    36361 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
    36421 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
    36481 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
    36541 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
    36601 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
    36661 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
    36721 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
    36781 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
    36841 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
    36901 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
    36961 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
    37021 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
    37081 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
    37141 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
    37201 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
    37261 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
    37321 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
    37381 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
    37441 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
    37501 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
    37561 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
    37621 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
    37681 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
    37741 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
    37801 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    37861 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    37921 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    37981 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    38041 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    38101 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    38161 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    38221 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    38281 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    38341 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    38401 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    38461 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    38521 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    38581 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    38641 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    38701 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    38761 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    38821 ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    38881 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    38941 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    39001 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    39061 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    39121 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    39181 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    39241 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    39301 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    39361 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    39421 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    39481 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    39541 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    39601 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    39661 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    39721 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    39781 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    39841 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    39901 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    39961 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    40021 ctattaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    40081 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    40141 tgtaatcggc accgtgtttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    40201 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    40261 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    40321 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    40381 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    40441 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    40501 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    40561 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    40621 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    40681 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    40741 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    40801 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    40861 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    40921 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    40981 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    41041 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    41101 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    41161 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    41221 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    41281 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    41341 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    41401 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    41461 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    41521 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    41581 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    41641 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    41701 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    41761 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    41821 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    41881 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    41941 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    42001 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    42061 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    42121 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    42181 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    42241 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    42301 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    42361 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    42421 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    42481 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    42541 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    42601 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    42661 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    42721 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    42781 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    42841 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    42901 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    42961 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    43021 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    43081 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    43141 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    43201 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    43261 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    43321 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    43381 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    43441 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    43501 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    43561 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    43621 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    43681 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    43741 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    43801 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    43861 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    43921 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    43981 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    44041 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    44101 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    44161 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    44221 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    44281 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    44341 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    44401 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    44461 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    44521 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    44581 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    44641 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    44701 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    44761 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    44821 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    44881 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    44941 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    45001 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    45061 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    45121 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    45181 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    45241 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    45301 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    45361 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    45421 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    45481 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    45541 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    45601 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    45661 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    45721 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    45781 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    45841 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    45901 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    45961 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    46021 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    46081 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    46141 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    46201 acgatggatg aaacacttct ttttgaattg gatcaatata atattcctat tgtggtaatc
    46261 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    46321 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    46381 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    46441 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    46501 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    46561 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    46621 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    46681 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    46741 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    46801 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    46861 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    46921 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    46981 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    47041 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    47101 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    47161 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    47221 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    47281 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    47341 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    47401 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    47461 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    47521 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    47581 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    47641 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    47701 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    47761 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    47821 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    47881 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    47941 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    48001 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    48061 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    48121 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    48181 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    48241 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    48301 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    48361 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    48421 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    48481 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    48541 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    48601 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    48661 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    48721 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    48781 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    48841 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    48901 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    48961 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    49021 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    49081 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    49141 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    49201 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    49261 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    49321 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    49381 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    49441 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    49501 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    49561 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    49621 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    49681 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    49741 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    49801 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    49861 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    49921 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    49981 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    50041 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    50101 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    50161 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    50221 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    50281 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    50341 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    50401 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    50461 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    50521 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    50581 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    50641 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    50701 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    50761 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    50821 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    50881 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    50941 acagaacaac ccttcacgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    51001 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    51061 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    51121 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    51181 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    51241 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    51301 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    51361 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    51421 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    51481 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    51541 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    51601 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    51661 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    51721 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    51781 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    51841 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    51901 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    51961 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    52021 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    52081 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    52141 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    52201 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    52261 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    52321 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    52381 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    52441 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    52501 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    52561 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    52621 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    52681 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    52741 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    52801 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    52861 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    52921 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    52981 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    53041 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    53101 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    53161 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    53221 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    53281 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    53341 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    53401 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    53461 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    53521 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    53581 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    53641 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    53701 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    53761 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    53821 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    53881 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    53941 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    54001 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    54061 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    54121 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    54181 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    54241 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    54301 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    54361 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    54421 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    54481 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    54541 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    54601 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    54661 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    54721 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    54781 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    54841 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    54901 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    54961 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    55021 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    55081 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    55141 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    55201 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    55261 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    55321 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    55381 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    55441 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    55501 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    55561 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    55621 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    55681 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    55741 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    55801 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    55861 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    55921 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    55981 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    56041 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    56101 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    56161 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    56221 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    56281 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    56341 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    56401 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    56461 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    56521 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    56581 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    56641 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    56701 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    56761 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    56821 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    56881 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    56941 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    57001 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    57061 atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct
    57121 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    57181 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    57241 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    57301 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    57361 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    57421 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    57481 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    57541 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    57601 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    57661 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    57721 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    57781 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    57841 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    57901 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    57961 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    58021 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    58081 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    58141 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    58201 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    58261 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    58321 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    58381 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    58441 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    58501 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    58561 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    58621 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    58681 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    58741 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    58801 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    58861 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    58921 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    58981 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    59041 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    59101 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    59161 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    59221 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    59281 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    59341 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    59401 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    59461 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    59521 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    59581 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    59641 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    59701 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    59761 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    59821 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    59881 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    59941 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    60001 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    60061 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    60121 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    60181 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    60241 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    60301 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    60361 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    60421 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    60481 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    60541 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    60601 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    60661 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    60721 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    60781 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    60841 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    60901 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    60961 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    61021 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    61081 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    61141 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    61201 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    61261 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    61321 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    61381 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    61441 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    61501 tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga
    61561 tagcacggat acatcagctt caagcga
//
LOCUS       DACECJ010000007        61432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.007, whole genome shotgun sequence.
ACCESSION   DACECJ010000007 DACECJ010000000
VERSION     DACECJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.007"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..563)
                     /locus_tag="IWO09_000417"
     CDS             complement(<1..563)
                     /locus_tag="IWO09_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0263038.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IWO09_000418"
     CDS             complement(750..1400)
                     /locus_tag="IWO09_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263039.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IWO09_000419"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IWO09_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0263040.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IWO09_000420"
     CDS             complement(2912..3910)
                     /locus_tag="IWO09_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0263041.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IWO09_000421"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IWO09_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0263042.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IWO09_000422"
     CDS             complement(4926..5657)
                     /locus_tag="IWO09_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0263043.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IWO09_000423"
     CDS             complement(5661..6926)
                     /locus_tag="IWO09_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0263044.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IWO09_000424"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IWO09_000424"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0263045.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IWO09_000425"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IWO09_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0263046.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IWO09_000426"
     CDS             12268..13770
                     /locus_tag="IWO09_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0263047.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IWO09_000427"
     CDS             13772..14509
                     /locus_tag="IWO09_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263048.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="IWO09_000428"
     CDS             complement(14554..14727)
                     /locus_tag="IWO09_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0263049.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="IWO09_000429"
     CDS             14703..15368
                     /locus_tag="IWO09_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0263050.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="IWO09_000430"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="IWO09_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0263051.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="IWO09_000431"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="IWO09_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0263052.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="IWO09_000432"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="IWO09_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0263053.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="IWO09_000433"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="IWO09_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0263054.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /locus_tag="IWO09_000434"
     CDS             complement(20581..21747)
                     /locus_tag="IWO09_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0263055.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="IWO09_000435"
     CDS             complement(21763..21990)
                     /locus_tag="IWO09_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0263056.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23064
                     /locus_tag="IWO09_000436"
     CDS             22474..23064
                     /locus_tag="IWO09_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0263057.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23254..23967
                     /locus_tag="IWO09_000437"
     CDS             23254..23967
                     /locus_tag="IWO09_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263058.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="IWO09_000438"
     CDS             complement(24082..25092)
                     /locus_tag="IWO09_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0263059.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="IWO09_000439"
     CDS             complement(25129..25611)
                     /locus_tag="IWO09_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0263060.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="IWO09_000440"
     CDS             25761..26129
                     /locus_tag="IWO09_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0263061.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="IWO09_000441"
     CDS             26594..27046
                     /locus_tag="IWO09_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0263062.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="IWO09_000442"
     CDS             complement(27170..28021)
                     /locus_tag="IWO09_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0263063.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="IWO09_000443"
     CDS             complement(28034..28819)
                     /locus_tag="IWO09_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0263064.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="IWO09_000444"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="IWO09_000444"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0263065.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="IWO09_000445"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="IWO09_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0263066.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="IWO09_000446"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="IWO09_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34217..34390
                     /locus_tag="IWO09_000447"
     CDS             34217..34390
                     /locus_tag="IWO09_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0263067.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="IWO09_000448"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="IWO09_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0263068.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="IWO09_000449"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="IWO09_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0263069.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="IWO09_000450"
     CDS             complement(37240..37551)
                     /locus_tag="IWO09_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0263070.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="IWO09_000451"
     CDS             complement(37548..37841)
                     /locus_tag="IWO09_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0263071.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="IWO09_000452"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="IWO09_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0263072.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="IWO09_000453"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="IWO09_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0263073.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="IWO09_000454"
     CDS             complement(39679..44037)
                     /locus_tag="IWO09_000454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0263074.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="IWO09_000455"
     CDS             complement(44249..45958)
                     /locus_tag="IWO09_000455"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0263075.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="IWO09_000456"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="IWO09_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0263076.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="IWO09_000457"
     CDS             complement(47455..48255)
                     /locus_tag="IWO09_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0263077.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="IWO09_000458"
     CDS             complement(48252..49064)
                     /locus_tag="IWO09_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0263078.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="IWO09_000459"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="IWO09_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0263079.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /locus_tag="IWO09_000460"
     CDS             complement(49819..50541)
                     /locus_tag="IWO09_000460"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0263080.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /locus_tag="IWO09_000461"
     CDS             complement(50677..51558)
                     /locus_tag="IWO09_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0263081.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="IWO09_000462"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="IWO09_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0263082.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="IWO09_000463"
     CDS             52798..53277
                     /locus_tag="IWO09_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0263083.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="IWO09_000464"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="IWO09_000464"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0263084.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="IWO09_000465"
     CDS             54578..54706
                     /locus_tag="IWO09_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0263085.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="IWO09_000466"
     CDS             54710..55255
                     /locus_tag="IWO09_000466"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0263086.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /locus_tag="IWO09_000467"
     CDS             complement(55709..57400)
                     /locus_tag="IWO09_000467"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0263087.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="IWO09_000468"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="IWO09_000468"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0263088.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="IWO09_000469"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="IWO09_000469"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0263089.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="IWO09_000470"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="IWO09_000470"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0263090.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17082 a        13109 c        10626 g        20615 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacatttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg tcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacatagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    61381 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt tt
//
LOCUS       DACECJ010000008        60193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.008, whole genome shotgun sequence.
ACCESSION   DACECJ010000008 DACECJ010000000
VERSION     DACECJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.008"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWO09_000471"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWO09_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0263091.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWO09_000472"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWO09_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0263092.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWO09_000473"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWO09_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0263093.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWO09_000474"
     CDS             1642..2274
                     /locus_tag="IWO09_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0263094.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWO09_000475"
     CDS             complement(2276..3181)
                     /locus_tag="IWO09_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0263095.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWO09_000476"
     CDS             complement(3266..3919)
                     /locus_tag="IWO09_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0263096.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWO09_000477"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWO09_000477"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0263097.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWO09_000478"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWO09_000478"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0263098.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWO09_000479"
     CDS             6168..7253
                     /locus_tag="IWO09_000479"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0263099.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWO09_000480"
     CDS             7250..8314
                     /locus_tag="IWO09_000480"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0263100.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWO09_000481"
     CDS             complement(8563..9366)
                     /locus_tag="IWO09_000481"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0263101.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWO09_000482"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWO09_000482"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ0263102.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWO09_000483"
     CDS             complement(10244..11251)
                     /locus_tag="IWO09_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ0263103.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWO09_000484"
     CDS             complement(11258..11902)
                     /locus_tag="IWO09_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0263104.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWO09_000485"
     CDS             complement(12002..12760)
                     /locus_tag="IWO09_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ0263105.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWO09_000486"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWO09_000486"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ0263106.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWO09_000487"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWO09_000487"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ0263107.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWO09_000488"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWO09_000488"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ0263108.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWO09_000489"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWO09_000489"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ0263109.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWO09_000490"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWO09_000490"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ0263110.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IWO09_000491"
     CDS             18158..19027
                     /locus_tag="IWO09_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0263111.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IWO09_000492"
     CDS             complement(19153..20052)
                     /locus_tag="IWO09_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0263112.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IWO09_000493"
     CDS             complement(20217..21041)
                     /locus_tag="IWO09_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0263113.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IWO09_000494"
     CDS             complement(21052..22347)
                     /locus_tag="IWO09_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ0263114.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IWO09_000495"
     CDS             complement(22363..22842)
                     /locus_tag="IWO09_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0263115.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IWO09_000496"
     CDS             complement(22848..23876)
                     /locus_tag="IWO09_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ0263116.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IWO09_000497"
     CDS             complement(23893..24999)
                     /locus_tag="IWO09_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0263117.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWO09_000498"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWO09_000498"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0263118.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWO09_000499"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWO09_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0263119.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWO09_000500"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWO09_000500"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0263120.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="IWO09_000501"
     CDS             29970..30668
                     /locus_tag="IWO09_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0263121.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="IWO09_000502"
     CDS             complement(30836..33475)
                     /locus_tag="IWO09_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0263122.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IWO09_000503"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IWO09_000503"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0263123.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="IWO09_000504"
     CDS             35318..36493
                     /locus_tag="IWO09_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0263124.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="IWO09_000505"
     CDS             36497..37138
                     /locus_tag="IWO09_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263125.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="IWO09_000506"
     CDS             37135..38052
                     /locus_tag="IWO09_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0263126.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="IWO09_000507"
     CDS             38049..38720
                     /locus_tag="IWO09_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263127.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="IWO09_000508"
     CDS             complement(39261..39749)
                     /locus_tag="IWO09_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0263128.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="IWO09_000509"
     CDS             complement(39950..40216)
                     /locus_tag="IWO09_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0263129.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="IWO09_000510"
     CDS             complement(40216..40479)
                     /locus_tag="IWO09_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0263130.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(40472..40744)
                     /locus_tag="IWO09_000511"
     CDS             complement(40472..40744)
                     /locus_tag="IWO09_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263131.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            40955..41140
                     /locus_tag="IWO09_000512"
     CDS             40955..41140
                     /locus_tag="IWO09_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0263132.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="IWO09_000513"
     CDS             complement(41282..43105)
                     /locus_tag="IWO09_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0263133.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="IWO09_000514"
     CDS             complement(43703..46408)
                     /locus_tag="IWO09_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0263134.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="IWO09_000515"
     CDS             complement(46714..47661)
                     /locus_tag="IWO09_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0263135.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="IWO09_000516"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="IWO09_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0263136.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="IWO09_000517"
     CDS             complement(48296..48658)
                     /locus_tag="IWO09_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0263137.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="IWO09_000518"
     CDS             complement(48669..49790)
                     /locus_tag="IWO09_000518"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0263138.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="IWO09_000519"
     CDS             complement(49806..50156)
                     /locus_tag="IWO09_000519"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0263139.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="IWO09_000520"
     CDS             complement(50286..51797)
                     /locus_tag="IWO09_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0263140.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="IWO09_000521"
     CDS             complement(52119..53471)
                     /locus_tag="IWO09_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0263141.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="IWO09_000522"
     CDS             complement(53624..54700)
                     /locus_tag="IWO09_000522"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0263142.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="IWO09_000523"
     CDS             54896..56641
                     /locus_tag="IWO09_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0263143.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="IWO09_000524"
     CDS             complement(56921..57358)
                     /locus_tag="IWO09_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0263144.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="IWO09_000525"
     CDS             complement(57342..58715)
                     /locus_tag="IWO09_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263145.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="IWO09_000526"
     CDS             complement(59037..59525)
                     /locus_tag="IWO09_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0263146.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="IWO09_000527"
     CDS             complement(59547..60149)
                     /locus_tag="IWO09_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0263147.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19084 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ctt
//
LOCUS       DACECJ010000009        57915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.009, whole genome shotgun sequence.
ACCESSION   DACECJ010000009 DACECJ010000000
VERSION     DACECJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.009"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWO09_000528"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWO09_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0263148.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWO09_000529"
     CDS             852..1040
                     /locus_tag="IWO09_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0263149.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWO09_000530"
     CDS             1053..1610
                     /locus_tag="IWO09_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0263150.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWO09_000531"
     CDS             1642..1884
                     /locus_tag="IWO09_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0263151.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2225..3439
                     /locus_tag="IWO09_000532"
     CDS             2225..3439
                     /locus_tag="IWO09_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0263152.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3470..4897
                     /locus_tag="IWO09_000533"
     CDS             3470..4897
                     /locus_tag="IWO09_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0263153.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4920..5846
                     /gene="glsA"
                     /locus_tag="IWO09_000534"
     CDS             4920..5846
                     /gene="glsA"
                     /locus_tag="IWO09_000534"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0263154.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6054..7514
                     /locus_tag="IWO09_000535"
     CDS             6054..7514
                     /locus_tag="IWO09_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263155.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7735..9498
                     /locus_tag="IWO09_000536"
     CDS             7735..9498
                     /locus_tag="IWO09_000536"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0263156.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9582..10040
                     /locus_tag="IWO09_000537"
     CDS             9582..10040
                     /locus_tag="IWO09_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0263157.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10149..10832
                     /locus_tag="IWO09_000538"
     CDS             10149..10832
                     /locus_tag="IWO09_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0263158.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10979..11593
                     /locus_tag="IWO09_000539"
     CDS             10979..11593
                     /locus_tag="IWO09_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0263159.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11609..11815
                     /locus_tag="IWO09_000540"
     CDS             11609..11815
                     /locus_tag="IWO09_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263160.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12354..12911
                     /locus_tag="IWO09_000541"
                     /pseudo
     CDS             12354..12911
                     /locus_tag="IWO09_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12969..15008
                     /locus_tag="IWO09_000542"
     CDS             12969..15008
                     /locus_tag="IWO09_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0263161.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15117..16268)
                     /locus_tag="IWO09_000543"
     CDS             complement(15117..16268)
                     /locus_tag="IWO09_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0263162.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16422..16952)
                     /locus_tag="IWO09_000544"
     CDS             complement(16422..16952)
                     /locus_tag="IWO09_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263163.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17051..17401)
                     /locus_tag="IWO09_000545"
     CDS             complement(17051..17401)
                     /locus_tag="IWO09_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0263164.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="IWO09_000546"
     CDS             complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="IWO09_000546"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0263165.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="IWO09_000547"
     CDS             complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="IWO09_000547"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0263166.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19804..20466)
                     /locus_tag="IWO09_000548"
     CDS             complement(19804..20466)
                     /locus_tag="IWO09_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0263167.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20650..22296
                     /locus_tag="IWO09_000549"
     CDS             20650..22296
                     /locus_tag="IWO09_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0263168.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22287..23369
                     /gene="uxuA"
                     /locus_tag="IWO09_000550"
     CDS             22287..23369
                     /gene="uxuA"
                     /locus_tag="IWO09_000550"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0263169.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23477..25030)
                     /locus_tag="IWO09_000551"
                     /pseudo
     CDS             complement(23477..25030)
                     /locus_tag="IWO09_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            25178..26319
                     /locus_tag="IWO09_000552"
                     /pseudo
     CDS             25178..26319
                     /locus_tag="IWO09_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26445..27524)
                     /locus_tag="IWO09_000553"
     CDS             complement(26445..27524)
                     /locus_tag="IWO09_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0263170.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27627..28067)
                     /locus_tag="IWO09_000554"
     CDS             complement(27627..28067)
                     /locus_tag="IWO09_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0263171.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28124..28534)
                     /locus_tag="IWO09_000555"
     CDS             complement(28124..28534)
                     /locus_tag="IWO09_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0263172.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28714..29148)
                     /locus_tag="IWO09_000556"
     CDS             complement(28714..29148)
                     /locus_tag="IWO09_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0263173.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29248..30096)
                     /locus_tag="IWO09_000557"
     CDS             complement(29248..30096)
                     /locus_tag="IWO09_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0263174.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30485..32251
                     /locus_tag="IWO09_000558"
     CDS             30485..32251
                     /locus_tag="IWO09_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0263175.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32353..33612
                     /locus_tag="IWO09_000559"
     CDS             32353..33612
                     /locus_tag="IWO09_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0263176.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33759..35060
                     /locus_tag="IWO09_000560"
     CDS             33759..35060
                     /locus_tag="IWO09_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0263177.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            35063..35914
                     /locus_tag="IWO09_000561"
     CDS             35063..35914
                     /locus_tag="IWO09_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0263178.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            35927..36742
                     /locus_tag="IWO09_000562"
     CDS             35927..36742
                     /locus_tag="IWO09_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0263179.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            36893..38059
                     /locus_tag="IWO09_000563"
     CDS             36893..38059
                     /locus_tag="IWO09_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0263180.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38289..38936
                     /locus_tag="IWO09_000564"
     CDS             38289..38936
                     /locus_tag="IWO09_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0263181.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            39020..39964
                     /locus_tag="IWO09_000565"
     CDS             39020..39964
                     /locus_tag="IWO09_000565"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0263182.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40099..41550
                     /locus_tag="IWO09_000566"
     CDS             40099..41550
                     /locus_tag="IWO09_000566"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0263183.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41571..42407
                     /locus_tag="IWO09_000567"
     CDS             41571..42407
                     /locus_tag="IWO09_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0263184.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42407..43066
                     /locus_tag="IWO09_000568"
     CDS             42407..43066
                     /locus_tag="IWO09_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0263185.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43192..43881
                     /locus_tag="IWO09_000569"
     CDS             43192..43881
                     /locus_tag="IWO09_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263186.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            43887..44843
                     /locus_tag="IWO09_000570"
     CDS             43887..44843
                     /locus_tag="IWO09_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0263187.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            44887..45399
                     /locus_tag="IWO09_000571"
     CDS             44887..45399
                     /locus_tag="IWO09_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0263188.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45655..46203)
                     /locus_tag="IWO09_000572"
     CDS             complement(45655..46203)
                     /locus_tag="IWO09_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0263189.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46292..47473)
                     /locus_tag="IWO09_000573"
     CDS             complement(46292..47473)
                     /locus_tag="IWO09_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0263190.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47709..49217)
                     /locus_tag="IWO09_000574"
     CDS             complement(47709..49217)
                     /locus_tag="IWO09_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0263191.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49376..49567
                     /locus_tag="IWO09_000575"
     CDS             49376..49567
                     /locus_tag="IWO09_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263192.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49770..50687
                     /locus_tag="IWO09_000576"
     CDS             49770..50687
                     /locus_tag="IWO09_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0263193.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50680..51753
                     /locus_tag="IWO09_000577"
     CDS             50680..51753
                     /locus_tag="IWO09_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263194.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            51901..52419
                     /locus_tag="IWO09_000578"
     CDS             51901..52419
                     /locus_tag="IWO09_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263195.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52400..52792
                     /locus_tag="IWO09_000579"
     CDS             52400..52792
                     /locus_tag="IWO09_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0263196.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            52868..53803
                     /locus_tag="IWO09_000580"
     CDS             52868..53803
                     /locus_tag="IWO09_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0263197.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            53936..54715
                     /locus_tag="IWO09_000581"
     CDS             53936..54715
                     /locus_tag="IWO09_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0263198.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54708..55025
                     /locus_tag="IWO09_000582"
     CDS             54708..55025
                     /locus_tag="IWO09_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0263199.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55269..56879
                     /locus_tag="IWO09_000583"
     CDS             55269..56879
                     /locus_tag="IWO09_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0263200.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            57036..57884
                     /locus_tag="IWO09_000584"
     CDS             57036..57884
                     /locus_tag="IWO09_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0263201.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19269 a        10229 c        11304 g        17113 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     2101 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     2161 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     2221 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2281 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2341 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2401 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2461 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2521 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2581 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2641 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2701 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2761 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2821 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2881 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2941 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     3001 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     3061 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     3121 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     3181 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3241 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3301 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3361 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3421 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     3481 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     3541 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     3601 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     3661 ggcaacggtg aaaggatggg aaaaaggcgg tgtctatact tgggtcagtc gcacattggg
     3721 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     3781 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     3841 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     3901 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     3961 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     4021 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     4081 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     4141 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     4201 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     4261 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     4321 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     4381 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     4441 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     4501 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     4561 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     4621 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     4681 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     4741 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     4801 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     4861 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     4921 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     4981 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     5041 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     5101 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     5161 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     5221 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     5281 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     5341 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     5401 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     5461 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     5521 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     5581 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     5641 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     5701 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     5761 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     5821 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     5881 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     5941 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     6001 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     6061 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     6121 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     6181 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     6241 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     6301 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     6361 cgagtacaga actggctgat cttgaattta tttctttgag caaagtatat acaatcaaaa
     6421 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     6481 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     6541 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     6601 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag
     6661 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     6721 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     6781 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     6841 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     6901 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     6961 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     7021 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     7081 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     7141 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     7201 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     7261 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     7321 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     7381 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     7441 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     7501 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     7561 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     7621 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     7681 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     7741 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     7801 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     7861 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     7921 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     7981 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     8041 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     8101 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     8161 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
     8221 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
     8281 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
     8341 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
     8401 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
     8461 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
     8521 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
     8581 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
     8641 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
     8701 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
     8761 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
     8821 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
     8881 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
     8941 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
     9001 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
     9061 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
     9121 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
     9181 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
     9241 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
     9301 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
     9361 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
     9421 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
     9481 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
     9541 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
     9601 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
     9661 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
     9721 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
     9781 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
     9841 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
     9901 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
     9961 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    10021 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    10081 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    10141 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    10201 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    10261 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    10321 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    10381 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    10441 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    10501 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    10561 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    10621 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    10681 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    10741 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    10801 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    10861 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    10921 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    10981 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    11041 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    11101 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    11161 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    11221 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    11281 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    11341 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    11401 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    11461 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    11521 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    11581 acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga
    11641 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    11701 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    11761 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    11821 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    11881 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    11941 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    12001 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    12061 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    12121 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    12181 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    12241 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    12301 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
    12361 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
    12421 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
    12481 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
    12541 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
    12601 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
    12661 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
    12721 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
    12781 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
    12841 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
    12901 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
    12961 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
    13021 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
    13081 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
    13141 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
    13201 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
    13261 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
    13321 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
    13381 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
    13441 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
    13501 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
    13561 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
    13621 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
    13681 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
    13741 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
    13801 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
    13861 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
    13921 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
    13981 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca
    14041 gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
    14101 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
    14161 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
    14221 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
    14281 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
    14341 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
    14401 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
    14461 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
    14521 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
    14581 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
    14641 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
    14701 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
    14761 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
    14821 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
    14881 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
    14941 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
    15001 atcaataaaa aatttctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
    15061 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
    15121 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
    15181 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
    15241 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
    15301 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
    15361 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
    15421 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
    15481 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
    15541 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
    15601 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
    15661 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
    15721 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
    15781 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
    15841 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
    15901 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
    15961 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
    16021 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
    16081 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
    16141 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
    16201 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
    16261 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
    16321 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
    16381 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
    16441 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
    16501 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
    16561 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
    16621 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
    16681 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
    16741 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
    16801 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
    16861 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
    16921 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
    16981 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
    17041 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
    17101 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
    17161 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
    17221 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
    17281 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
    17341 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
    17401 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
    17461 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
    17521 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
    17581 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
    17641 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
    17701 tcactatttc atttcttgga ttcatttcga tcgttctatc tagccaatcc gtattaggga
    17761 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
    17821 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
    17881 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
    17941 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
    18001 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
    18061 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
    18121 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
    18181 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
    18241 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
    18301 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
    18361 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
    18421 ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt tctttttcaa
    18481 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
    18541 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
    18601 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
    18661 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
    18721 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
    18781 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
    18841 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
    18901 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
    18961 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
    19021 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
    19081 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
    19141 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
    19201 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
    19261 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
    19321 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
    19381 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
    19441 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
    19501 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
    19561 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
    19621 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
    19681 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
    19741 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
    19801 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
    19861 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
    19921 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
    19981 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
    20041 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
    20101 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
    20161 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
    20221 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
    20281 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
    20341 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
    20401 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
    20461 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
    20521 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
    20581 catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg ttagtttagg
    20641 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
    20701 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
    20761 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
    20821 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
    20881 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
    20941 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
    21001 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
    21061 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
    21121 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
    21181 attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
    21241 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
    21301 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
    21361 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
    21421 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
    21481 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
    21541 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
    21601 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
    21661 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
    21721 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
    21781 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
    21841 ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
    21901 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
    21961 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
    22021 ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca taagctttcc
    22081 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
    22141 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    22201 gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt acaggattac
    22261 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    22321 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    22381 cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa gaactaaaag
    22441 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    22501 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    22561 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    22621 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    22681 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    22741 aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc ttgcttgaag
    22801 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    22861 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    22921 actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat tggttgtgtc
    22981 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    23041 cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga atcccatttg
    23101 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccacataaat
    23161 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    23221 acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    23281 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    23341 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    23401 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    23461 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    23521 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    23581 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    23641 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    23701 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    23761 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    23821 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    23881 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaata
    23941 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    24001 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    24061 caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt gaataaagaa
    24121 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    24181 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    24241 ttggcagatt aggtcgcaaa atatctacag agagatcaat aaaaaagtca atatattcat
    24301 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    24361 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    24421 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    24481 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    24541 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtactatg
    24601 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    24661 gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    24721 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    24781 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    24841 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    24901 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    24961 gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta atcgcttctt
    25021 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    25081 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    25141 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    25201 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    25261 gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    25321 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    25381 catcaaagca aaaattgact attcgggaga cttagcacct atactacaat cagctgttac
    25441 atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt tgactatcca
    25501 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    25561 gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa cgccgtatag
    25621 tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc ttgttgaaga
    25681 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
    25741 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
    25801 gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg taactccaga
    25861 accagatacg gattctggaa accaaacagt accggaaaca aatccagaca cagataatga
    25921 aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg tgactccaga
    25981 accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa aaacaattca
    26041 agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa taaatgaaga
    26101 gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa ccttaaaaag
    26161 tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc aaaatccgga
    26221 gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat agcaacaatg
    26281 ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa tgtctgtgaa
    26341 cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca gattgaaatc
    26401 tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg gatatctgtt
    26461 tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa gaaggtaaag
    26521 atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac aagtaagacc
    26581 acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg attcagcaca
    26641 taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac atctcggatg
    26701 cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac acctgctact
    26761 gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg aaagccaaat
    26821 acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa ttgagcttct
    26881 aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc ttgaaagaac
    26941 caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt cttttcaatc
    27001 ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt tcctatatcc
    27061 tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc
    27121 tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa
    27181 aaattaataa cggactggat catgtcaaat gtttgatgaa taagcatcgg gatatacttt
    27241 gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc taaaacacct
    27301 gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag
    27361 ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac aaaaacgaca
    27421 aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg ataaacggtc
    27481 aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg atggaatatg
    27541 aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga gttggttttc
    27601 ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg aaaattatct
    27661 agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag tggaagataa
    27721 gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc ttgagcagtc
    27781 agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat ataatttttt
    27841 tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa atcatttcct
    27901 agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag atactgggta
    27961 taggtaagat tcagcggctt gaggagggga gtgtagtgtc taatgatttc tttagaggcg
    28021 gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt tccacctccg
    28081 ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca ctaactgctt
    28141 tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact ttatgcgctg
    28201 cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct tcaatctcta
    28261 cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca ctgacagtag
    28321 aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta aaacatgcac
    28381 tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag
    28441 ctgcaatatt caattcgaat gtacgatcgg gattatgcac ttctccagag cgtccgcctg
    28501 tgttgatcat tttcgtagaa tacatttttt tcataaagtt accaccttta attatatttt
    28561 atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt ggcataagat
    28621 aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa
    28681 tatcagagac aaaaaataat ctcatcataa acattacata tatttctcaa aaagttgttt
    28741 tctttcttca aaaattactt cacgatggac ctgatgaagc atccataaat aaccaaaagg
    28801 atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt cttgaatctc
    28861 tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga cgatgatatt
    28921 gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag acgtgttttc
    28981 atccagcata tggaatctcg taccatacaa ggtaaaaatg acttcattat tgcctctagg
    29041 gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca gttctagtgc
    29101 tttcaaacta tcatttaata caaaatcgat ttccgttcct ttatgcataa agatctcctc
    29161 tccttttcag tcgaacagtt actagattct tttattatac cagctaaaga aaacgcattc
    29221 aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa atccgcgtcc
    29281 cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt cttctatcaa
    29341 tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct cttttgactt
    29401 tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct cagcacttaa
    29461 gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt tctttcgatt
    29521 gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata aaatagcata
    29581 aagaaactct tctattccaa aaataaacag attaagagaa acaacgaatg cgtctgtaaa
    29641 aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg gagggatcgt
    29701 cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag caaaaatcgc
    29761 acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg gaactattgc
    29821 aatgattaaa ggaaaaacca agcttccgaa aagtacttta aaaaagggtt ttttacctaa
    29881 aaatagaaga gctagtacca acataacgat attcaacact aaaataacta tagctcggtc
    29941 aaagcctaaa gcagcttcca gtaatattcc tagaccgctg acacctccag cagcaatatg
    30001 atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa aaataatctt
    30061 aatgtatgtc catataaatt gtctcgtttc tttcatttat tcacttcctt aattcattaa
    30121 taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa ttaataaaaa
    30181 aattaggcta gtctaaatta ttatgtttca ttcttctaga atatacttct acgccaaaaa
    30241 ctgttaaaaa acttttactt tagaaaattg gagaaattga tcgcttaaga taaattcagg
    30301 acactaaata gtacaggaag tagactaaaa cttttaaaag ttttaagtct atctatttgg
    30361 caagtttaaa aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc
    30421 gttgtctttt gcttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa
    30481 atcaatggat acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac
    30541 gaatacaaga tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt
    30601 gctatgtgga gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat
    30661 gaaaaaatgt ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac
    30721 aagtcgctta cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg
    30781 tgatcaagga atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg
    30841 aataccttat tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt
    30901 atggtatcaa atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca
    30961 cactttacct tggggaagta aagatccaga ccgagaagac tttttcgggg gagatttgca
    31021 aggtgtgatg gatcatttgg attacttgac ggaccttggc atcaatggca tctatttttg
    31081 tcccatattt aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga
    31141 tcccgctttt ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg
    31201 gatacgtatc atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca
    31261 agacgttctc aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt
    31321 cccagtagat tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga
    31381 tacttttgct tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa
    31441 atatttgctg gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct
    31501 tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga
    31561 aatcaaacca gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca
    31621 aggggatgaa ttccatgcag tgatgaatta tgcatttaca gattcgatta aagattattt
    31681 tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct
    31741 ttacagagat caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag
    31801 gatcttgacc ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt
    31861 tcttcaaaaa gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga
    31921 tgacccggat tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt
    31981 cggttttata aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg
    32041 ttctactcag tggctaatag taaatgatcg tgaagataag ctctatttca cacggaaact
    32101 agagggacag atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca
    32161 ggaaaatgag gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat
    32221 acgacaatac ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg
    32281 tttgcaaatg aagatttgtg cccattataa taagggtgta acagcacgtt gaatacaaag
    32341 gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta
    32401 ggaatcttag gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc
    32461 gatgaaggaa aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa
    32521 ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg
    32581 ttcgatcagt tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg
    32641 atggcagcat atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa
    32701 ctgggaaatg aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa
    32761 atctacggag aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc
    32821 gatacagcac catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct
    32881 tttgagtcag aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat
    32941 tcctatggtt taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct
    33001 tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg
    33061 ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc
    33121 cagcaattca tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca
    33181 tacaaagaaa acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag
    33241 ccttatcaac cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa
    33301 gacctctctc aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt
    33361 gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac
    33421 gatgaattaa caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc
    33481 ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt
    33541 tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa
    33601 caaaaatact gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca
    33661 gataaaagtg gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa
    33721 gcagtttgcg gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca
    33781 atatcaacga aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa
    33841 atggccaagc ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga
    33901 tgcttctggg cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg
    33961 aagatcattc attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt
    34021 tcttgatttt ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag
    34081 agggcaaaaa aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt
    34141 ttccttatct attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag
    34201 tcctagttac attgttcact gcatttacta attatgactt caatcatatt ccaccagcca
    34261 gtttaattga ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc
    34321 gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt
    34381 tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca
    34441 aacgtatttt cggtatcatt ttcttattac catgggcggt cccagctttt attactatca
    34501 tgagtttttc gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt
    34561 tcataaacca gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact
    34621 ggacgaaagt agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga
    34681 tggttacagg aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg
    34741 gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag
    34801 cgccaacatt tattacacag tatacgggga atttcaataa tttctccatg atctatctgt
    34861 ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt
    34921 cctggatcta taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca
    34981 cgttgattat ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg
    35041 cgttcaatat gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg
    35101 actttctcac ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc
    35161 gcttttgatc acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga
    35221 tctttcaagt gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg
    35281 gacttggtat aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt
    35341 agtaactttg gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagttt
    35401 gatgttcttt ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt
    35461 catggctttc ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg
    35521 cggtggaatt ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga
    35581 tttagatgaa tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt
    35641 attaccgctt gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt
    35701 tggggatttc atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt
    35761 cggcttacaa acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg
    35821 ggcaattttg attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt
    35881 taccggcctg acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc
    35941 gttaaactat ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt
    36001 atcatggcct aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt
    36061 gactcttttt tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca
    36121 ttcgctgatc gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg
    36181 gttacaagca tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt
    36241 atctgagaaa caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt
    36301 gatccaacaa aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt
    36361 ccaaccagat tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta
    36421 tgccagtaat cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt
    36481 tgtcatgacg agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag
    36541 ccaactttca tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg
    36601 aataggttcc atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct
    36661 aagtgtccaa acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt
    36721 caaagataag cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa
    36781 aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc
    36841 ttttcttttt tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga
    36901 gaaaacttta tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta
    36961 ttgggacggt acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa
    37021 tatccatgaa gaaatcccaa tgaaagagat tacaagtaac gcttctttgg cactaggaga
    37081 agcctatatg gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc
    37141 ttatcagcaa gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa
    37201 agaaaaacat acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa
    37261 cgacttttat aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac
    37321 acctgaagat acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact
    37381 ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt
    37441 cactgctacc aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata
    37501 cgatttgatt accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt
    37561 aatggattac cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt
    37621 cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa
    37681 accaaaagga acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc
    37741 ttggatcaat aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga
    37801 taatattaca aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca
    37861 attgacatta gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga
    37921 aatgggtgat tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt
    37981 tgaatcaagt aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat
    38041 cccaatgcat cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc
    38101 taagttttat gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa
    38161 gcttagtttc tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca
    38221 tgacagaaaa ttttttcttc gttatacttg taatagaata ctattttata acaaagggga
    38281 tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg
    38341 cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc
    38401 gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa
    38461 aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg
    38521 aattgttcca agataaatac ccagatactg caattactag tttggaatta gatagtgatt
    38581 ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa
    38641 ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac
    38701 aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg
    38761 aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg
    38821 ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat
    38881 cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat
    38941 ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt
    39001 gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa
    39061 tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc
    39121 tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa
    39181 tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa
    39241 cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca
    39301 aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct
    39361 tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa
    39421 tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga
    39481 agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc
    39541 tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat
    39601 caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc
    39661 aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt
    39721 tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg
    39781 gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac
    39841 cgagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat
    39901 tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc
    39961 atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct
    40021 gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga
    40081 ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa
    40141 gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat
    40201 gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt
    40261 ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa
    40321 gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct
    40381 catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc
    40441 aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga
    40501 aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt
    40561 tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc
    40621 gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca
    40681 tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga
    40741 gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta
    40801 tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt
    40861 gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta
    40921 gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt
    40981 aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg
    41041 attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact
    41101 ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg
    41161 gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca
    41221 acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca
    41281 cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg
    41341 tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca
    41401 gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg
    41461 gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat
    41521 cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa
    41581 aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg
    41641 ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag
    41701 cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt
    41761 atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag
    41821 aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga
    41881 acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat
    41941 attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag
    42001 atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa
    42061 aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg
    42121 tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat
    42181 ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa
    42241 gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa
    42301 aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg
    42361 tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcctct
    42421 aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac
    42481 attagggatt ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc ttttgacagt
    42541 tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt
    42601 tagaggcatt cccgcactag tcctgctgtt tctgttatat tttgggttac cttatcaatt
    42661 aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga
    42721 aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc
    42781 tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc
    42841 agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac
    42901 aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta
    42961 tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa
    43021 agtgcaacag tatttagaaa aacgttctat gcatcagtca atctgaaaaa tagaaatggt
    43081 gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta
    43141 aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc
    43201 ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg
    43261 gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg
    43321 acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat
    43381 tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt
    43441 aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt
    43501 ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca
    43561 gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta
    43621 ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaacagat
    43681 caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga
    43741 tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa
    43801 gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg
    43861 cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg
    43921 acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag
    43981 tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa
    44041 agcaccatcc aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc
    44101 agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa
    44161 cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac
    44221 agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt
    44281 tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg
    44341 actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta
    44401 gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg
    44461 aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt
    44521 atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg
    44581 ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc
    44641 gtcaccatca tcaactcttg attacttcag gaatgattga attattggct ctttaccagc
    44701 aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac
    44761 ttattagtca cctgctagat catccaaaag attggcttaa atattattca aaagttcagg
    44821 aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga
    44881 aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt
    44941 aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa
    45001 ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg
    45061 gaagatcaaa ttgctggaac agctgcatta gtctcaagcg tggatcccgt ttacacaaaa
    45121 atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta
    45181 gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt
    45241 gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg
    45301 gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa
    45361 cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa
    45421 gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag
    45481 aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg
    45541 aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg
    45601 acatagcttt tgtcacgacc tctcggctta tcctacgagt attattcgat acatttatat
    45661 caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac
    45721 taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc
    45781 cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt
    45841 ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg
    45901 ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat
    45961 ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt
    46021 tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt
    46081 ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat
    46141 agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt
    46201 cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa
    46261 gcttttaaaa gtaactttgc ttttttgtat attataccag caagccagtc acaatagcac
    46321 ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat
    46381 ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt
    46441 tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat
    46501 taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga
    46561 tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa
    46621 agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga
    46681 tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga
    46741 aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa
    46801 tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat
    46861 aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca
    46921 tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca
    46981 aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc
    47041 ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg ttgattttac
    47101 ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac
    47161 cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc
    47221 ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat
    47281 gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata
    47341 cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt
    47401 ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta
    47461 ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa
    47521 ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt
    47581 tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag
    47641 tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta
    47701 aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg
    47761 attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct
    47821 tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact
    47881 gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga
    47941 agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg
    48001 tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc
    48061 atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca
    48121 gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg
    48181 actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga
    48241 ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga
    48301 cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct
    48361 acacgattca cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt
    48421 tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact
    48481 ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg
    48541 gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt
    48601 tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag
    48661 gaagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt
    48721 gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact
    48781 ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg
    48841 ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat
    48901 aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca
    48961 taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca
    49021 gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt
    49081 acacgtttcg catgcttgtc gtcaactagc ttcccaccaa attctaaata taatttgtca
    49141 tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca
    49201 aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt catttttttg
    49261 aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca
    49321 acttgtctag atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa
    49381 aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta
    49441 tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga
    49501 aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag
    49561 taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact
    49621 agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc
    49681 cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa
    49741 aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg
    49801 agccagacga actagaggag acgaatgcgg atcaggatac aggacaagaa tcgtcttctt
    49861 tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg
    49921 cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc
    49981 gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga
    50041 tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca
    50101 tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg
    50161 gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga
    50221 aatattccca accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg
    50281 tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac
    50341 aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa
    50401 ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt
    50461 atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag
    50521 cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg
    50581 gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg
    50641 agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca
    50701 gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa
    50761 gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa
    50821 atacacgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct
    50881 tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc
    50941 gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg
    51001 atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa
    51061 ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa
    51121 ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga
    51181 catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta
    51241 ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag
    51301 acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg
    51361 gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta
    51421 gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca
    51481 cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt
    51541 atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa
    51601 gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc
    51661 ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat caattacgaa
    51721 ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca
    51781 ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct
    51841 tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga
    51901 atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg
    51961 atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta
    52021 ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt
    52081 ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg
    52141 agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga
    52201 gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg
    52261 ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg
    52321 cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac
    52381 aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag
    52441 tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga
    52501 ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc
    52561 ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg agtattttga aacgaaattt
    52621 tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa
    52681 aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga
    52741 gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata
    52801 aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga
    52861 aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc
    52921 taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat
    52981 gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg
    53041 tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa
    53101 atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt
    53161 gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt
    53221 tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa
    53281 agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg
    53341 acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt
    53401 ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag
    53461 cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta
    53521 tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt
    53581 tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga
    53641 gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt
    53701 catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata
    53761 cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat
    53821 tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga
    53881 atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa
    53941 caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag
    54001 ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct
    54061 cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc
    54121 tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt
    54181 acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt
    54241 tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt
    54301 ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac
    54361 tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc
    54421 tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa
    54481 aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt
    54541 attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt
    54601 tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga
    54661 tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat
    54721 atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga
    54781 tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac
    54841 cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg
    54901 atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa
    54961 cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc
    55021 tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct
    55081 atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa
    55141 gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt
    55201 ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg
    55261 gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc
    55321 tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc
    55381 aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc
    55441 tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa
    55501 aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg
    55561 tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca
    55621 gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga
    55681 ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc
    55741 cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta
    55801 cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag
    55861 tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa
    55921 aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc
    55981 gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca
    56041 ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc
    56101 cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata
    56161 aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat
    56221 taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt
    56281 cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta gctggaccac
    56341 tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc
    56401 tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc
    56461 accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag
    56521 taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg
    56581 gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag
    56641 caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat
    56701 gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa
    56761 tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg
    56821 gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg
    56881 ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg
    56941 tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt
    57001 ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata
    57061 gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc
    57121 ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt
    57181 atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag
    57241 ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc
    57301 tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg
    57361 gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga cacatggtag
    57421 aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg
    57481 atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac
    57541 gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga
    57601 tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag
    57661 catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca
    57721 aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc
    57781 agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac
    57841 atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa
    57901 aacataccaa aaata
//
LOCUS       DACECJ010000010        57128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.010, whole genome shotgun sequence.
ACCESSION   DACECJ010000010 DACECJ010000000
VERSION     DACECJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.010"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..257)
                     /locus_tag="IWO09_000585"
     CDS             complement(<1..257)
                     /locus_tag="IWO09_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263202.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="IWO09_000586"
     CDS             complement(273..923)
                     /locus_tag="IWO09_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263203.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="IWO09_000587"
     CDS             complement(934..2106)
                     /locus_tag="IWO09_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0263204.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="IWO09_000588"
     CDS             complement(2125..2997)
                     /locus_tag="IWO09_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0263205.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="IWO09_000589"
     CDS             complement(3019..3939)
                     /locus_tag="IWO09_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263206.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="IWO09_000590"
     CDS             complement(3939..4208)
                     /locus_tag="IWO09_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263207.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="IWO09_000591"
     CDS             complement(4205..4585)
                     /locus_tag="IWO09_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAQ0263208.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="IWO09_000592"
     CDS             complement(5052..6515)
                     /locus_tag="IWO09_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0263209.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="IWO09_000593"
     CDS             complement(6579..9596)
                     /locus_tag="IWO09_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0263210.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIKKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="IWO09_000594"
     CDS             complement(9747..10586)
                     /locus_tag="IWO09_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0263211.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="IWO09_000595"
     CDS             10731..10976
                     /locus_tag="IWO09_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263212.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="IWO09_000596"
     CDS             11039..11461
                     /locus_tag="IWO09_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263213.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="IWO09_000597"
     CDS             complement(11555..11872)
                     /locus_tag="IWO09_000597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263214.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="IWO09_000598"
     CDS             complement(11899..12105)
                     /locus_tag="IWO09_000598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263215.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="IWO09_000599"
     CDS             complement(12086..12268)
                     /locus_tag="IWO09_000599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263216.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="IWO09_000600"
     CDS             complement(12282..12632)
                     /locus_tag="IWO09_000600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263217.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="IWO09_000601"
     CDS             complement(12658..12960)
                     /locus_tag="IWO09_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263218.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="IWO09_000602"
     CDS             complement(12963..13196)
                     /locus_tag="IWO09_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263219.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14182..14504)
                     /locus_tag="IWO09_000603"
                     /pseudo
     CDS             complement(14182..14504)
                     /locus_tag="IWO09_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14627..15742)
                     /locus_tag="IWO09_000604"
     CDS             complement(14627..15742)
                     /locus_tag="IWO09_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263220.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15748..16146)
                     /locus_tag="IWO09_000605"
     CDS             complement(15748..16146)
                     /locus_tag="IWO09_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263221.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17175..17561)
                     /locus_tag="IWO09_000606"
     CDS             complement(17175..17561)
                     /locus_tag="IWO09_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0263222.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17558..18079)
                     /locus_tag="IWO09_000607"
     CDS             complement(17558..18079)
                     /locus_tag="IWO09_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0263223.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWO09_000608"
     CDS             complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWO09_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0263224.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19148..20563)
                     /locus_tag="IWO09_000609"
     CDS             complement(19148..20563)
                     /locus_tag="IWO09_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ0263225.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20532..22685)
                     /locus_tag="IWO09_000610"
     CDS             complement(20532..22685)
                     /locus_tag="IWO09_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ0263226.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22943..23179
                     /locus_tag="IWO09_000611"
     CDS             22943..23179
                     /locus_tag="IWO09_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0263227.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23198..23413
                     /locus_tag="IWO09_000612"
     CDS             23198..23413
                     /locus_tag="IWO09_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0263228.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24116..24868)
                     /locus_tag="IWO09_000613"
     CDS             complement(24116..24868)
                     /locus_tag="IWO09_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0263229.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24883..26130)
                     /locus_tag="IWO09_000614"
     CDS             complement(24883..26130)
                     /locus_tag="IWO09_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0263230.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(26206..26352)
                     /locus_tag="IWO09_000615"
     CDS             complement(26206..26352)
                     /locus_tag="IWO09_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0263231.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(26456..26767)
                     /locus_tag="IWO09_000616"
     CDS             complement(26456..26767)
                     /locus_tag="IWO09_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0263232.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(26769..26966)
                     /locus_tag="IWO09_000617"
     CDS             complement(26769..26966)
                     /locus_tag="IWO09_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0263233.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(27360..29087)
                     /locus_tag="IWO09_000618"
     CDS             complement(27360..29087)
                     /locus_tag="IWO09_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0263234.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(29080..30273)
                     /locus_tag="IWO09_000619"
     CDS             complement(29080..30273)
                     /locus_tag="IWO09_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0263235.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(30460..31104)
                     /locus_tag="IWO09_000620"
     CDS             complement(30460..31104)
                     /locus_tag="IWO09_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0263236.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(31198..31332)
                     /locus_tag="IWO09_000621"
     CDS             complement(31198..31332)
                     /locus_tag="IWO09_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0263237.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="IWO09_000622"
     CDS             complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="IWO09_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0263238.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(33463..33936)
                     /locus_tag="IWO09_000623"
     CDS             complement(33463..33936)
                     /locus_tag="IWO09_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0263239.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            34337..34561
                     /gene="nrdH"
                     /locus_tag="IWO09_000624"
     CDS             34337..34561
                     /gene="nrdH"
                     /locus_tag="IWO09_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0263240.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            34720..36879
                     /gene="nrdE"
                     /locus_tag="IWO09_000625"
     CDS             34720..36879
                     /gene="nrdE"
                     /locus_tag="IWO09_000625"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0263241.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            36929..37894
                     /gene="nrdF"
                     /locus_tag="IWO09_000626"
     CDS             36929..37894
                     /gene="nrdF"
                     /locus_tag="IWO09_000626"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0263242.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(37983..39122)
                     /locus_tag="IWO09_000627"
     CDS             complement(37983..39122)
                     /locus_tag="IWO09_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0263243.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(39431..40144)
                     /locus_tag="IWO09_000628"
     CDS             complement(39431..40144)
                     /locus_tag="IWO09_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0263244.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="IWO09_000629"
     CDS             complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="IWO09_000629"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0263245.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            41333..42865
                     /locus_tag="IWO09_000630"
     CDS             41333..42865
                     /locus_tag="IWO09_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0263246.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(43056..44258)
                     /locus_tag="IWO09_000631"
     CDS             complement(43056..44258)
                     /locus_tag="IWO09_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0263247.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(44340..45029)
                     /locus_tag="IWO09_000632"
     CDS             complement(44340..45029)
                     /locus_tag="IWO09_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0263248.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="IWO09_000633"
     CDS             complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="IWO09_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0263249.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(45556..46059)
                     /locus_tag="IWO09_000634"
     CDS             complement(45556..46059)
                     /locus_tag="IWO09_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0263250.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="IWO09_000635"
     CDS             complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="IWO09_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0263251.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="IWO09_000636"
     CDS             complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="IWO09_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0263252.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            48027..48695
                     /gene="sdaAB"
                     /locus_tag="IWO09_000637"
     CDS             48027..48695
                     /gene="sdaAB"
                     /locus_tag="IWO09_000637"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0263253.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            48708..49580
                     /gene="sdaAA"
                     /locus_tag="IWO09_000638"
     CDS             48708..49580
                     /gene="sdaAA"
                     /locus_tag="IWO09_000638"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0263254.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(49671..50690)
                     /locus_tag="IWO09_000639"
     CDS             complement(49671..50690)
                     /locus_tag="IWO09_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0263255.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(50863..51750)
                     /locus_tag="IWO09_000640"
     CDS             complement(50863..51750)
                     /locus_tag="IWO09_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0263256.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="IWO09_000641"
     CDS             complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="IWO09_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0263257.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(52473..52643)
                     /gene="secE"
                     /locus_tag="IWO09_000642"
     CDS             complement(52473..52643)
                     /gene="secE"
                     /locus_tag="IWO09_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0263258.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="IWO09_000643"
     CDS             complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="IWO09_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0263259.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            52981..53625
                     /locus_tag="IWO09_000644"
     CDS             52981..53625
                     /locus_tag="IWO09_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0263260.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(53681..54184)
                     /locus_tag="IWO09_000645"
     CDS             complement(53681..54184)
                     /locus_tag="IWO09_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0263261.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(54197..54243)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            54781..55746
                     /locus_tag="IWO09_000646"
     CDS             54781..55746
                     /locus_tag="IWO09_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0263262.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(55845..57017)
                     /locus_tag="IWO09_000647"
     CDS             complement(55845..57017)
                     /locus_tag="IWO09_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263263.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(57004..>57128)
                     /locus_tag="IWO09_000648"
     CDS             complement(57004..>57128)
                     /locus_tag="IWO09_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263264.1"
                     /translation="LYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        17012 a        11482 c         9386 g        19248 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttttaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga atggctaaat
    15601 ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat gagcgatatt
    15661 tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat ttacgagttg
    15721 cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata ttaagaaaga
    15781 attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt ttagataaat
    15841 tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga tttcttcaat
    15901 ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag aaatatagac
    15961 atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact cattcagtaa
    16021 gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt ttataaagaa
    16081 tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt
    16141 taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca
    16201 cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa aactaaacac
    16261 ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa gagaaaaaga
    16321 tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat tcataatata
    16381 tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt tttatagtaa
    16441 atttatcaaa atttttgcca acattttttc cgattttttt ccctatttct atctttcctt
    16501 atctttctta gttaatatta tttgatgtta atatatcact actaatatta aaaaacattg
    16561 atttatcata ggataaagag tgatagttta atacttaaag agacatatta attttattta
    16621 aaaattgaca ttaatgactc ttaatcagcg ggtcgcgggt tcgagcccct cacggcccat
    16681 tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga aaacatttac
    16741 gctgttcttt ttttattttt caaaataact agggcttttt atttttaact caaagtccac
    16801 tgtagccctt tggactttga gttaaaaaag aagcataatt atgaacagta aagtttctaa
    16861 tataacaata attcgagaag ctttgcaaaa tcatgctgcc aatgaactct ctaaacatat
    16921 aggattaaat ctaagtacta taaaaggtta aaaagtgaag aatatttaat agaaaaacta
    16981 aatttacttg ctgctttccc cttttccatt aatacaacaa tcagagagtg tctttttttc
    17041 gaaaaaatta gaataaataa aagacacgta gtttttttat ctacatgtct tccatcgact
    17101 acaagttatc atttctcaaa acgtatttag cttttaattg attaatcgtt tcacgttgtc
    17161 tacacattgc ctaattaaga ttttcttatt agaagcatca atcccgcaat aatacctaaa
    17221 actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga taaaatgagt
    17281 aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc tattatgaaa
    17341 atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc gcttagattg
    17401 actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt tgaatcaata
    17461 gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc tgttattcca
    17521 ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc tccagattaa
    17581 gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa cgaataacaa
    17641 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
    17701 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
    17761 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
    17821 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
    17881 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
    17941 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
    18001 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
    18061 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
    18121 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
    18181 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
    18241 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
    18301 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
    18361 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
    18421 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
    18481 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
    18541 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
    18601 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
    18661 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
    18721 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
    18781 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
    18841 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
    18901 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
    18961 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
    19021 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
    19081 gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat attcttagta
    19141 aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat gttttttctc
    19201 ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat tgagttgttt
    19261 ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa ttttctgaaa
    19321 tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc aatttaaaat
    19381 gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt gctgtaaatt
    19441 tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct ttggctagtt
    19501 ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga gcttttagta
    19561 ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc tcttgttttt
    19621 catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc tcttgctttc
    19681 ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact aatttagctt
    19741 tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca tttatgcttg
    19801 ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt agttgttctt
    19861 gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat tgattattac
    19921 tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc tcaatttgtt
    19981 tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca tttaattcac
    20041 tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt aattgattac
    20101 tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata ctttcaataa
    20161 atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct atctgagcct
    20221 tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta tgaacaaatt
    20281 gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt aacttctcta
    20341 ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta gcaaaggtta
    20401 aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa cggtagaagc
    20461 tatgatgttg tttagtatag atactagaat gttcgaacca attattattc tgcataaatt
    20521 gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa acgaagttca
    20581 tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg atcacatgct
    20641 tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa attgaggaga
    20701 taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc atctaaaatt
    20761 aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg ttgctctcct
    20821 gaaagattat ttccaccttc ttccaacaat gtctcgtaac gtaatggctg ttgattaata
    20881 aattcattta gttggactat ctcacaaact tctattattt gttcgaaact tggtgggcta
    20941 tctaaaccga atataagatt ctcaatgatt gttccactaa agaaaaatga ctcttgaggc
    21001 aaatatgtca ctcgatcgcg taatatctga aaaggaatgt ctaagtgatt aatgtttcca
    21061 taatggatcg atccttctga tgcttcataa aaacgaacaa ataactttgc aagagttgat
    21121 tttccagaac cactaactcc aactatagcc gttttactgt gcgaaggaat tgtacatgaa
    21181 atttcgttca acgttggggc cttcatgcta tacgagaatg aaacgttttt tacagtgata
    21241 tcttgttgaa ataattgttc agaaagcata tattcaaaag actggttcga tttttctgaa
    21301 tcaatcgcga aaatttcatt taaacgatta tttgcaactt gagccgtttg cattttcact
    21361 tgtaaattaa taatgttttg taaaggatct gtcaaaaata ctagtaaagc attgtatgtg
    21421 ataagttgac ctagactaat tctattatcc ataacaaaat aagaacctag ccataaaatt
    21481 aatgcactgc taattaattg aatcaaatgt ttaattcctt gttgaatatt atcaagcgta
    21541 accgtacgaa atgatttttt cattaattta acaaattcat ggtctatacg gttatatact
    21601 ttctcttcac cgctatatgc tttaattgtt tctattcctt taagactttc tatgatacta
    21661 gaatttacag ctgcaccggc tgccatttct tccctgttcg atttttcata gcttttcacg
    21721 aaagcgaata ttgcaactac ataaaaaggc aaagagcata gagtgattaa aaacaacgta
    21781 ctattctgaa tagctaacgt aaccccaaca agcaacacca taccaatatc taaaaatatt
    21841 gataacgtag cacttgctaa agcatcaata atcttactgg catcaagaaa acgagaaata
    21901 atttcccctg attttctcgt agcaaaaaaa ttcattggta aagttaacac atgtttgaaa
    21961 tactgaagca taattgccat actcattcgc tgtcctaaaa taataagaag ataactacga
    22021 ctatattcaa acaatacccg aaataaatag acagcgatca agccaatgga aattatattt
    22081 aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa ataatatgag
    22141 cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa tactaatcct
    22201 ttttgtttcc aaattatagg caaaaaagaa cttaatccag ctactttttc tttaatagga
    22261 atataagttt ctctaggcat aggtaataaa agaatacctg tccattcttt ttgaaacgct
    22321 tcaatcgttt ttctattttt tccattagcg ggatctgcaa tccatagtac gtttcccttc
    22381 gttttataaa ttacaacgta gtgcatatag ctgttatcaa ccaatatatg tgcaattaat
    22441 ggtaagataa gatctctttc tttccagatc tcactatctg cttgaatagc catacaatca
    22501 aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc taaatcagtc
    22561 ccagataggt cccgtaattt atgaatcggg atatcagttt taaaatattt taaaatcatc
    22621 gaaaggcaag caacaccaca atctttttca tcttgttgct gcacaaaagg aaatgatttt
    22681 atcacttatc taacacaact ccttctaaaa aatttttata gaaaaattct caatatagct
    22741 ttaatacagt gaaaatattg tctcataatt ccacaaaaac tttgaaaaca gttcttgaac
    22801 actaactttt tcattcctga atcaaattta ttttggaaat tttactattc aagaataaac
    22861 ttgataagtt tcaggaagat ttttactgtt aatcaaatac gtatgttaca gtagcaccgt
    22921 aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa tgtaaaggaa ctaagtattg
    22981 tggaaatgca caaactgttg gtggaggcaa taatgcttgg ggaaaattgg ggcaagttgt
    23041 aggtggactt accacagggg cagtaggtgg tgcaggttta ggaacagcaa tttgcggacc
    23101 agcttgcggt gttgttggag ggttatatgg agctgtcgct ggaggtgctg cagctggctg
    23161 ggatgctcga aaaaaataaa aaaggagaat aagtagtatg catattaaaa acacaaaaac
    23221 aacatttata ttatcttcag aagagttgaa aaatattcaa ggtgggagtg ccgtgggagt
    23281 tttaggcact actttttctg gggcaacagc aggagtaaaa ctttgttcag ccggaggacc
    23341 ttatgcaata gctgcttgtg gtgtaggtgg agctttatta ggtgctggat tcagcatgtg
    23401 gaccggtgcg taaacaatta aaaaatatca aacctcaaga ttggtttgtg tcgattacga
    23461 acataggtat gttctttatc ttttcaaaag tttggaatgg acagaacttt ttactaaagc
    23521 ttcttgtgct agtttttata attatattaa atttttatct tttattaaag tcagataaaa
    23581 attctttgga aatgtaatgg tagagatatt tttacaaaag gacttgcagc gctgtagttg
    23641 gtgctgcagg aggacctatt tcggccgtta caggaggtat tggtaatgta cttgtcggat
    23701 gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa caggaaagta attatctcaa
    23761 atcctatcaa gtacaatcat aggattgcac ttgacaggac aaatttttta actaaaaaat
    23821 gaaaaacact ttctaaaaat tagtagaatt tgccaaatct ttcgcatctt ccaccataca
    23881 tttaatttta attccccttt atggtactta ataaaaacaa gaaaagaact attcatttgg
    23941 cccttttttg gttccagacc atagaaatat tcctccagta actaatattg ctgttatgca
    24001 cgtaactaat aacagctata aaaattggta caactttaat aggattcaga aaaatttgca
    24061 ctagtgaagg aatctatttt attagaaaaa tgcaatctcc ctaaagaaaa aaccactaaa
    24121 cagtgctttc tttcaacttc tccttaattt tttgtgcttt ccctaaagta aagtttctgg
    24181 ccaactctga atcaaaatat agtgaacgtt ttttaaaatc tatttcttta acgttaaaga
    24241 tatttactaa acacgctcga ctaattcttg ttaacatagg atatttcttt tcaagctcac
    24301 taattcttcc ataaaattcg tactgcccat ctttagtaca caatgataat ctatgtggta
    24361 attttgaagc ttctacaaaa taaatgtcat ttatatcaaa agtaaatgtt tgagaaccta
    24421 tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc atctatcctt tcttgcgcta
    24481 ataacaataa ctcaacaatc tcgtttcgat actcatctag tgtttgatct tttgttacaa
    24541 atcctaacgt ttcaactctt ctttttattg ttacgggtaa catctcatca tgagtagttg
    24601 taaaaatgat ttttgcttga acatcatatt ttctgattac ttctgctaat tctataccgt
    24661 taacttcatg atttaaatca atatctaaga aatatattcc ttgctttggt cggaattgtt
    24721 tgaggtattt tttaacttct aacggacttt gtgttttcaa tactatctta aatactttat
    24781 cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt ataatttgat
    24841 cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca acaaaataac
    24901 ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg gattcttttt
    24961 attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg ttgttattcc
    25021 ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg atatcgaaat
    25081 ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt gactctctat
    25141 agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt caaatattgg
    25201 aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct tattgttatt
    25261 tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta tattttttaa
    25321 ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta attcttttat
    25381 ttctttttcc aatatcgaat catggttttg attagcgatt tcttttaaac tcagcaataa
    25441 atttttatag tcatgtctaa atctggcaaa tttttcttga tctttttcca gtgaatcagt
    25501 atattttttt agataaacca attcttttct tttcaattgc tgttcatatt gttctcgttg
    25561 cctaatagtt attctaataa ataaaaatag aacaacaaac atttgaatga ttaaaaatat
    25621 taagacacca aacacaaaat gatcaaatac ttgatattga tgagcaacat aagaaacaaa
    25681 taaaccaatt gtaagtaaat aaatcatgaa tatagcagta agtttagaac tggactgttc
    25741 aacaatccta tttatagcta attttctata taaccaaata aacatcagca aaaacaaaga
    25801 atcaagcatt atctggaata ctacataacc ataccctttt atcccttcaa atgaaaacat
    25861 atacgtcata accatggaag taataataga tctgaaatca tcaataagca tacataatag
    25921 taatttattt aaaagcacta tattagtttt cttcttttcc tttaatataa aataacttcc
    25981 gattataggt atagctgcgg aaacatcagt aaataattca acaaaaaaag tgagtggtat
    26041 caataaacat acaaaaagat attttctttt gttaagatca gtattagtca tataaaataa
    26101 gagaaaaaag aaaaagtttc ccacgaacat tcttaataga taagcttttg tatcgatagt
    26161 aatcaagaga tcccctcctg attttaggat atcataaaaa aaattttagt tacacttttt
    26221 aaataatgaa aaaacacatt ccactaaatt tgatgccggt tttccttgag gttttgttcc
    26281 agcacctcct ttaatttttt taagctcttg atctgaacat tgtttagcat ttagtctatt
    26341 tttttcttcc attacgattc cccctacaaa ctataaatac ttttatgtca ctgattcaat
    26401 atacctattt ttctaatcaa aaaattttga gacatattta atattataag tatcattaaa
    26461 attgagattt atctccataa tctgctcgat ataaaccatt tattccagca gttcttccaa
    26521 tttcactcaa atttcttatt aattcctctt gatattcagt gaatcttatt ttattagtat
    26581 aagcagtaaa atatagataa ttcaccaatc tatttattaa aggaccggga tctaattcat
    26641 atttttgttt ttctaatttt aaatatacct tttgtaaaac ctcaagaata tcagaatatt
    26701 taggatcttt acttatagat atatcattat ataattcatg aacaatttgc ttagcatttt
    26761 ttttcatttt agcacttccc tggaattgct ccacctaaaa aaccacctat agacattcct
    26821 gcaatacaag tagttgcctt ggcccaatcg accgtacatt tatttttagt gcaatacact
    26881 ccatttccat aatattttcc actatgagtg gtacccccat agataagttg tgtctcttta
    26941 atagacaaaa tttttaaatg tttcataatc taatcacccc taaattaatg tttttagact
    27001 attattctaa cattcattaa atttcaaaaa acaattcctt cttgaaacgt agcaaaataa
    27061 ttcctgaata agaatttaat cacttttaag tatataaact atgtatattc tttaatattt
    27121 tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat
    27181 cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt tggacgtttc
    27241 gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc
    27301 ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt
    27361 tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc
    27421 tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa
    27481 tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc
    27541 atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga
    27601 tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt
    27661 ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag
    27721 tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct
    27781 ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca
    27841 tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct
    27901 tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa
    27961 accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc
    28021 gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca
    28081 acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa
    28141 aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct
    28201 tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc
    28261 aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca
    28321 aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt
    28381 gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc
    28441 cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt
    28501 gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa
    28561 ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc
    28621 atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc
    28681 ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc
    28741 cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta
    28801 ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat taatgtgaac
    28861 aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata
    28921 aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca
    28981 atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt
    29041 tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc
    29101 gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc
    29161 atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac
    29221 caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt
    29281 ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct
    29341 gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg
    29401 tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac
    29461 tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa
    29521 tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc
    29581 cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt
    29641 ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga
    29701 gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac
    29761 ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact
    29821 agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac
    29881 ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg
    29941 gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga
    30001 tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag
    30061 acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc
    30121 ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc
    30181 cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat
    30241 ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc
    30301 atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa
    30361 aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa
    30421 ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc
    30481 cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat
    30541 gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga
    30601 aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc
    30661 cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg
    30721 tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc
    30781 ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc
    30841 ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc
    30901 atgccgtact tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc
    30961 aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt
    31021 atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg
    31081 gacgattttt ttctttccag acattttctt tcctctttct ttttttactt tccctcatct
    31141 tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca
    31201 tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc
    31261 ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa
    31321 gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg
    31381 attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg
    31441 tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa
    31501 ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca
    31561 tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta
    31621 acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc
    31681 cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt
    31741 tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct
    31801 gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag
    31861 gttgagcata aaaattataa gaagagctga accagatcac gatactagaa acaaagataa
    31921 tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg
    31981 accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa
    32041 acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat
    32101 aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa
    32161 tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt
    32221 gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca
    32281 gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa
    32341 aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa
    32401 tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca
    32461 gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta
    32521 accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa
    32581 tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat
    32641 tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa
    32701 cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac
    32761 tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag
    32821 aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat
    32881 accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt
    32941 ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct
    33001 gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg
    33061 gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct tttttgatga
    33121 catccatcat attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta
    33181 aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt
    33241 cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga
    33301 tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt
    33361 ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca
    33421 agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct
    33481 ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt
    33541 cagttcgatt ggatctccca gaggggctag ttttcggata actattttta catttcgcgt
    33601 caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat
    33661 cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt
    33721 tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac
    33781 cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat
    33841 cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata
    33901 aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa
    33961 atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat
    34021 gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata
    34081 ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt
    34141 ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc
    34201 ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt
    34261 gttgtttgac ttttttacta agagttttat actatctatt atagataata agaatgtgtt
    34321 agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa
    34381 gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga
    34441 acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac
    34501 ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta
    34561 acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt
    34621 ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat
    34681 caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa
    34741 gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct
    34801 ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg
    34861 atgacttttc tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa
    34921 gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac
    34981 gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg
    35041 ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat
    35101 gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc
    35161 catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc
    35221 gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca
    35281 atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat
    35341 cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca
    35401 gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga
    35461 gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa
    35521 aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct
    35581 gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt
    35641 gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat
    35701 ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa
    35761 atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac
    35821 cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa
    35881 gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc
    35941 tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa
    36001 tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc
    36061 gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc
    36121 ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt
    36181 acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct
    36241 cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat
    36301 ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt
    36361 gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa
    36421 gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc
    36481 aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag
    36541 aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac
    36601 acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa
    36661 cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt
    36721 tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac
    36781 gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa
    36841 gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta
    36901 ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg
    36961 gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg
    37021 gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt
    37081 ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc
    37141 tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt
    37201 gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac
    37261 actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct
    37321 gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa
    37381 aattgccagc gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta
    37441 ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga
    37501 tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca acgagttacc
    37561 tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga
    37621 aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa
    37681 gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt
    37741 tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag
    37801 caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat
    37861 gaaagacgac gattatttga ttggattgga ataaggattt attttaattc aaaaattttt
    37921 cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat
    37981 gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc
    38041 attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag
    38101 acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc
    38161 aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa
    38221 tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga
    38281 gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg
    38341 ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt
    38401 ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata
    38461 taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct
    38521 ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc
    38581 aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt
    38641 gagacacttg cagataaact gactaatatg atattgaaca aattactaag agataaattt
    38701 gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca
    38761 tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc
    38821 tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc
    38881 aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg
    38941 attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa
    39001 agcattttca atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg
    39061 atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc
    39121 atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg
    39181 ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg
    39241 agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt
    39301 ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata
    39361 taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct attttacaac
    39421 ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg
    39481 acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat
    39541 ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga
    39601 tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt
    39661 ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa
    39721 tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc
    39781 ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg
    39841 tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc
    39901 ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg
    39961 caccactagc gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa
    40021 tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc
    40081 caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat
    40141 tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc
    40201 aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg
    40261 aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg
    40321 agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    40381 tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca
    40441 ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc
    40501 cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc
    40561 ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag
    40621 aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca
    40681 aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg
    40741 atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct
    40801 tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca
    40861 tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag
    40921 tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta
    40981 actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg
    41041 atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata
    41101 gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt tctttttaca
    41161 ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt
    41221 gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg
    41281 attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat
    41341 actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga
    41401 tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag
    41461 tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca
    41521 aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat
    41581 cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt
    41641 tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact
    41701 ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag
    41761 cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg
    41821 acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt
    41881 agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca
    41941 ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac
    42001 cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca
    42061 agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca
    42121 aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac
    42181 gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc
    42241 agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga
    42301 aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat
    42361 ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt
    42421 aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac
    42481 aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca
    42541 atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa
    42601 agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca
    42661 aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga
    42721 accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt
    42781 tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg
    42841 agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg
    42901 acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg
    42961 gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac
    43021 ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc
    43081 tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta
    43141 accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc
    43201 gcacctccaa taaagtacat aaacataaat acagtattat ttcggttgct tgctgcttca
    43261 cctagccctt gtactcgtgt ttgattggcg acttgaccaa attgattccc gacgtctaat
    43321 aaaataatag ctaaaagcaa aatggccaaa tggcttccgc caaccaataa agcaataaag
    43381 cttattgttt gcataacaag tcccatatac acgatttttc tttcagaata agaatctgcc
    43441 agtcgtccca cgataggtgc agcaactgct ccagacaaac caaaaatcgc taatatccct
    43501 gcttcgaatg ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa
    43561 aagatcgaaa atgttccaaa catgaaaaat ccactgactg ccgcttctct taatagacgt
    43621 tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac agctgtacta
    43681 ctttgctgga cagaacgcgg cattttccag taaagcagaa gtgtaagaat tccgactgca
    43741 cataaagcaa ataaatagat catccgccaa gaaaaaagac tggcaacaat accagatacc
    43801 gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca atgtacccat gaccttccct
    43861 cttgcttttg gtcccgctag taccgcaccg taaggaatga tgatttgcgg cacgatcgaa
    43921 agtaatccta ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg
    43981 gccaacagag agacggcaga taacgctgcc attcgaatga tcaattttgg acgatccacc
    44041 acgtccccta aaggaactaa aaacagtaac cctaacgcat agcctaattg ggtcaacatc
    44101 gtcaatatcc cgactgcact tgtgctaaca gataaactag ctgctacttt tgttccaatc
    44161 ggctggatgt aatacatatt ggcaacaaca acgccacaag tgattgataa tagtaaaata
    44221 agcgaagttg ttaaaggttt tttttcatta ctcttcattg atttccccca tttcctatct
    44281 tagtttacaa cttatggaaa agattggaaa tagaaaaata caactattcg ccacacttat
    44341 tatttgtgct tttgttttcc ttgttttttc ttgcgatctt tataaatgaa gacaactaac
    44401 cctactagca cagtaacact tgcaaccaac gtgactgcag ttttgttata tccgtgaaac
    44461 agagcttctc caccacacgc atacaacaca gacgaaggga tgactccaat cacgatcgac
    44521 aatatgattt gctgtagagg caatttcatc gcatctgcag caaaattgac catagaagat
    44581 ggaataaccg gtatcatata gcctaacatt acacctattt ttggatgttt catttgtcgg
    44641 attgcttgca cccaatgatt cgtttcggtt ttcttatcta aaaattttaa gcgatgcata
    44701 aggaaaattg cagtgatgtt accaagagca ttacccaaaa tgttgatgat cgcacctgtc
    44761 agtggtccaa agctcaaacc agcaatgact ccaactactg aagtaggaat acctggaaca
    44821 gcacacatca ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc atgcgaacga
    44881 atatgtgcga gtagctctgc cttactcgct ctcggattta aaaataattg gatgtctgtc
    44941 tgatattcta aatacaaccg atagcccact accagtatca gaatgattcc caacgagatc
    45001 aatatcaatc ggattatttt ctttttcata tttcctcctg aaaaaaatct atattcatta
    45061 aataatagtt taacataaaa acaaaaaagc accgaaatcc aacataggat ctcagtgctt
    45121 ttaaatagta aaaattattt aacagttaca gatgcgccaa cttcttcaag tttagctttc
    45181 aattcttcag cttcttcttt agatacgcct tctttaactg gtgcaggagc gccatcaact
    45241 acagctttag cttctttcaa gcctaaacca gttgcttcac gaactgcttt gataacttta
    45301 actttttggt ctccagctgc agttaattct acagtgaatt ctgtttgttc ttcagcagca
    45361 gcagcgccac cagcagcagc tacagctaca ggagcagcag cagatacgcc aaattcttct
    45421 tcaatagctt taactaggtc gttaagttct aggatagttg cttcttttaa ctcagcaacg
    45481 atgttttcaa tgttcaatgc cattataatt tcctccgttt aattgttctt ttttatttaa
    45541 tgctttatgc ggcaattaag ctgcatctcc atcttctttg tctgccactg ctttgacagc
    45601 gtaagccacg ttgcggactg gcgcttgtag tacagatagc aacatagata gaagtccttc
    45661 gcgatttggc aattttgcca atgctgtgat ttcttccaat gaagagactt taccttcgat
    45721 gataccgcct ttgatttcta atgcttttgc ttctttagca aattcgtcca tgattttcgc
    45781 tggtgcgact acatcttcgt tactgaatgc aacagctgta ggtccagtga atacttcgtc
    45841 catgccttca aggccagctt ttttagctgc acgtgaaaga atagaatttt tgataacttt
    45901 catttctacg ttagcatcac gaagttgttt acgtaaacgt gttacttcat caactgttaa
    45961 tccgcggtag tcaacgacta ctactgatgc agctgctgta aatttttcgc ttacttcatc
    46021 aacgattgct gctttttttg cgataattgc ttcactcatt tttgagtttc acctcctgaa
    46081 ttttgatgga agagtttttt gcactgaaaa ggcaagtcac ttttatagtt actttgctca
    46141 accagtatta ttcatcacct attcacttcg ttcatagtga ttctactcgc tttgctcgaa
    46201 aggaaactcc tttcgctttg ctcaaccagt attgttcatc atctcttcac ttcgttcata
    46261 gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt gaatatattc
    46321 aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc ggtacaagtt
    46381 aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat tatttttcag
    46441 ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca tggataccag
    46501 gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct gctgatggtt
    46561 tagcttttag caatacatca tggattgctg caaagttttc aactaatttt tcatcgtcaa
    46621 atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg taagttactt
    46681 taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta ccagttttag
    46741 ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca gtagccatca
    46801 tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt ttttgaacca
    46861 tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt
    46921 tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca acagcaccac
    46981 ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact tcaactgttg
    47041 catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca acaggataaa
    47101 ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt ttagccattt
    47161 tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta tatctttcaa
    47221 tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac agtgatcccc
    47281 atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc tgcgtttagg
    47341 tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt cgcaacttta
    47401 tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa tagaacagca
    47461 gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga aataacaacc
    47521 ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt gaatcccatg
    47581 atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt tgctttacct
    47641 gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca tacctcctta
    47701 agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta tctgatctga
    47761 aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag caaatttatt
    47821 tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac gctaaattta
    47881 ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac ttgcgctagc
    47941 atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt acaaagattc
    48001 ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt gtctttgata
    48061 ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct gctagaatcg
    48121 ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt tatctatatg
    48181 aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt ggcggcctgt
    48241 tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca tatgaagccg
    48301 gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac tctgtaaaga
    48361 tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc ggcggtggca
    48421 atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt actcctacct
    48481 tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt atactttctg
    48541 agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt gaaaatgcca
    48601 ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc
    48661 tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg tttttatcga
    48721 ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg atgattgcag
    48781 ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc
    48841 tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa
    48901 ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac tttttaagtg
    48961 gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa
    49021 tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga
    49081 tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc ttatttaccg
    49141 ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca gaagggggat
    49201 gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa
    49261 atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat atgatgggac
    49321 tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag
    49381 gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa agtgtcattc
    49441 cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg tcgattttca
    49501 aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt gcggaagaga
    49561 ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga ttagttatct
    49621 tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta ttaatccatc
    49681 aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat cgtgatcgac
    49741 aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc tagattcaac
    49801 ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag agctttgaag
    49861 acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt gttgactact
    49921 tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctagttg acgtccacca
    49981 tgattggata cccagatacc atctgcccct gcagagatag cgattactgc atcttctggc
    50041 gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt tttgatgtca
    50101 tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt tccttcgcca
    50161 ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa tggaaattgg
    50221 aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg
    50281 atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa tctgtcgtct
    50341 ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg
    50401 attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc tactccttta
    50461 gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg cgcttggata
    50521 atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga gtcgattccg
    50581 cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc tttcatcgtc
    50641 cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat gcgctctttt
    50701 acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc attacttgta
    50761 tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc atactaaact
    50821 tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt tccaaataaa
    50881 tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag aggcattttc
    50941 acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg catagaccgc
    51001 taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta gttgaccagc
    51061 aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt aatcttgata
    51121 gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact gcgcactatc
    51181 atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt gtggcaatga
    51241 cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca tggaatgccc
    51301 cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac aagttttgat
    51361 ccattctgct tgattccact cgttattttt attatcttca aacaatactt ggaccatcgg
    51421 attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg ttcctactgt
    51481 taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca tccctttaaa
    51541 ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat
    51601 cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat ttatcttcgt
    51661 cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg cagttgcacg
    51721 tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat agaaaaaaga
    51781 aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa tcgattttat
    51841 ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg atgttgactt
    51901 tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc
    51961 cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt cgcaggctca
    52021 taccgataga acgcaagata tgattgactt cttctggaag taatggtgct ggtttgcttc
    52081 cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca acataccatg
    52141 aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta tgaacgattt
    52201 ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg acacggaaaa
    52261 taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt actttgtttt
    52321 catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc attgaaggct
    52381 cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct ttcgcttcat
    52441 tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc aaataagctt
    52501 tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt ttcgatgact
    52561 actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt gatttcatct
    52621 tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat ttcgtttctt
    52681 tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa cgttcgccgc
    52741 tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg
    52801 ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact tatacttcat
    52861 aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta ttgttttgga
    52921 taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg aggtatagcg
    52981 atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt
    53041 acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta
    53101 gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa
    53161 gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc
    53221 atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt
    53281 gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat
    53341 atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca
    53401 ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc
    53461 agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta
    53521 ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat
    53581 tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa
    53641 agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat
    53701 caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac
    53761 ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat
    53821 aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta
    53881 catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc
    53941 caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc
    54001 ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa
    54061 tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa
    54121 gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt
    54181 gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt
    54241 tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc
    54301 ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta
    54361 taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa
    54421 gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc
    54481 ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa
    54541 cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct
    54601 tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca
    54661 gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt
    54721 ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc
    54781 atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa
    54841 gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat
    54901 agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt
    54961 ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga
    55021 ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt agaattccca
    55081 gttgcttata ttccagcagg ttctggagat gattttgctc gaggggcagg ccttccaaaa
    55141 aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata
    55201 atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata
    55261 ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac
    55321 aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa
    55381 aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt
    55441 ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct
    55501 gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc
    55561 ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac
    55621 tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga
    55681 gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg
    55741 tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag
    55801 gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa
    55861 ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc
    55921 gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg
    55981 taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat
    56041 gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata
    56101 aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc
    56161 aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa
    56221 atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt
    56281 aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc
    56341 aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaat
    56401 gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa
    56461 ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca
    56521 acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta
    56581 tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact
    56641 gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcggcatctt tcctacttcg
    56701 acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga
    56761 gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc
    56821 aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc
    56881 gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca
    56941 acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc
    57001 gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag
    57061 gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa
    57121 ataaagtt
//
LOCUS       DACECJ010000011        56523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.011, whole genome shotgun sequence.
ACCESSION   DACECJ010000011 DACECJ010000000
VERSION     DACECJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.011"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO09_000649"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO09_000649"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0263265.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IWO09_000650"
     CDS             complement(937..1518)
                     /locus_tag="IWO09_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0263266.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO09_000651"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO09_000651"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0263267.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO09_000652"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO09_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0263268.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO09_000653"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO09_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0263269.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IWO09_000654"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IWO09_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0263270.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IWO09_000655"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IWO09_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0263271.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO09_000656"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO09_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0263272.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IWO09_000657"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IWO09_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0263273.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IWO09_000658"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IWO09_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0263274.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IWO09_000659"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IWO09_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0263275.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IWO09_000660"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IWO09_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0263276.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO09_000661"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO09_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0263277.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IWO09_000662"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IWO09_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0263278.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO09_000663"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO09_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0263279.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IWO09_000664"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IWO09_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0263280.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO09_000665"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO09_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0263281.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO09_000666"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO09_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0263282.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IWO09_000667"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IWO09_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0263283.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IWO09_000668"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IWO09_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0263284.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IWO09_000669"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IWO09_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0263285.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IWO09_000670"
     CDS             13972..14157
                     /locus_tag="IWO09_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0263286.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO09_000671"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO09_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0263287.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IWO09_000672"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IWO09_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0263288.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IWO09_000673"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IWO09_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0263289.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO09_000674"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO09_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0263290.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO09_000675"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO09_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0263291.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IWO09_000676"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IWO09_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0263292.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IWO09_000677"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IWO09_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0263293.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IWO09_000678"
     CDS             18232..18879
                     /locus_tag="IWO09_000678"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0263294.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IWO09_000679"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IWO09_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0263295.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO09_000680"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO09_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0263296.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO09_000681"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO09_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0263297.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO09_000682"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO09_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0263298.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IWO09_000683"
     CDS             20317..21255
                     /locus_tag="IWO09_000683"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0263299.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO09_000684"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO09_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0263300.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IWO09_000685"
     CDS             21935..22774
                     /locus_tag="IWO09_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0263301.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IWO09_000686"
     CDS             22750..23619
                     /locus_tag="IWO09_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0263302.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IWO09_000687"
     CDS             23609..24406
                     /locus_tag="IWO09_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0263303.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IWO09_000688"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IWO09_000688"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0263304.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IWO09_000689"
     CDS             complement(25688..28357)
                     /locus_tag="IWO09_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0263305.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IWO09_000690"
     CDS             complement(28595..28783)
                     /locus_tag="IWO09_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0263306.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IWO09_000691"
     CDS             29053..29415
                     /locus_tag="IWO09_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0263307.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IWO09_000692"
     CDS             29467..31149
                     /locus_tag="IWO09_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0263308.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IWO09_000693"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IWO09_000693"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0263309.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IWO09_000694"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IWO09_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0263310.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IWO09_000695"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IWO09_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0263311.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IWO09_000696"
     CDS             34984..35988
                     /locus_tag="IWO09_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263312.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IWO09_000697"
     CDS             35989..36933
                     /locus_tag="IWO09_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263313.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IWO09_000698"
     CDS             36933..37895
                     /locus_tag="IWO09_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263314.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IWO09_000699"
     CDS             37922..38836
                     /locus_tag="IWO09_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263315.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IWO09_000700"
     CDS             38862..40643
                     /locus_tag="IWO09_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0263316.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IWO09_000701"
     CDS             40991..41677
                     /locus_tag="IWO09_000701"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0263317.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IWO09_000702"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IWO09_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0263318.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IWO09_000703"
     CDS             45287..46096
                     /locus_tag="IWO09_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0263319.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IWO09_000704"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IWO09_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0263320.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IWO09_000705"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IWO09_000705"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0263321.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="IWO09_000706"
     CDS             complement(49648..50100)
                     /locus_tag="IWO09_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0263322.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="IWO09_000707"
     CDS             50250..51200
                     /locus_tag="IWO09_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0263323.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="IWO09_000708"
     CDS             51193..52152
                     /locus_tag="IWO09_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0263324.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="IWO09_000709"
     CDS             52149..52904
                     /locus_tag="IWO09_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0263325.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="IWO09_000710"
     CDS             52927..53880
                     /locus_tag="IWO09_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0263326.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54403..54699)
                     /locus_tag="IWO09_000711"
     CDS             complement(54403..54699)
                     /locus_tag="IWO09_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0263327.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55054..55410
                     /locus_tag="IWO09_000712"
     CDS             55054..55410
                     /locus_tag="IWO09_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0263328.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55420..56319
                     /locus_tag="IWO09_000713"
     CDS             55420..56319
                     /locus_tag="IWO09_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0263329.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        18544 a        10222 c        12115 g        15642 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg tccagcgttc
    54301 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt caatcagctg
    54361 accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat gtgtctcagc
    54421 aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa gcttactcca
    54481 ccgttttaat aattgataga aataactctc ttctgctttg attttatact cgcttaatat
    54541 caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca atgctttggt
    54601 tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga tattttgtgt
    54661 ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc caaagaagtc
    54721 ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac aaaaagtaat
    54781 tctttttcca atagttgtta aaggtttttt attttttata gtattttttg ttatttaaga
    54841 aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt tcaagaaact
    54901 gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat aaacgaatga
    54961 cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca tctgatgaat
    55021 aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac aaaagaagca
    55081 caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga tatcgaaaac
    55141 ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc atttcgattg
    55201 atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt ggctgataca
    55261 ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga tccatccaca
    55321 cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg cttgagaaaa
    55381 ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt ggcgatcatc
    55441 agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca gctttggcaa
    55501 gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc taataaagta
    55561 gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac gacatttcat
    55621 ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca ttttccagac
    55681 ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct tttagggatg
    55741 aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg
    55801 aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct cgaggtcaat
    55861 gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag
    55921 gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca atctgctcct
    55981 tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg agaattactt
    56041 gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg ctttgaagat
    56101 aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta tgaatggcag
    56161 ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc tatgaaatta
    56221 cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca acatttgcaa
    56281 gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag taatgtgtaa
    56341 aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt tatcgtggat
    56401 atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat gatttgtaac
    56461 tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac aaaaaaaaat
    56521 ata
//
LOCUS       DACECJ010000012        52140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.012, whole genome shotgun sequence.
ACCESSION   DACECJ010000012 DACECJ010000000
VERSION     DACECJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.012"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            330..1175
                     /locus_tag="IWO09_000714"
     CDS             330..1175
                     /locus_tag="IWO09_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0263330.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IWO09_000715"
     CDS             1456..2676
                     /locus_tag="IWO09_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0263331.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IWO09_000716"
     CDS             2673..5393
                     /locus_tag="IWO09_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0263332.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IWO09_000717"
     CDS             5377..6321
                     /locus_tag="IWO09_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0263333.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IWO09_000718"
     CDS             complement(6390..7400)
                     /locus_tag="IWO09_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0263334.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IWO09_000719"
     CDS             complement(7580..7924)
                     /locus_tag="IWO09_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0263335.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IWO09_000720"
     CDS             complement(7917..8351)
                     /locus_tag="IWO09_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0263336.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IWO09_000721"
     CDS             8489..9226
                     /locus_tag="IWO09_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263337.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IWO09_000722"
     CDS             9219..10430
                     /locus_tag="IWO09_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263338.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IWO09_000723"
     CDS             10637..11416
                     /locus_tag="IWO09_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0263339.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IWO09_000724"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IWO09_000724"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0263340.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO09_000725"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IWO09_000725"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0263341.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO09_000726"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IWO09_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0263342.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IWO09_000727"
     CDS             15018..16067
                     /locus_tag="IWO09_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0263343.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IWO09_000728"
     CDS             16064..16342
                     /locus_tag="IWO09_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0263344.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IWO09_000729"
     CDS             16317..16787
                     /locus_tag="IWO09_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0263345.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17052
                     /locus_tag="IWO09_000730"
     CDS             16804..17052
                     /locus_tag="IWO09_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263346.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNM"
     gene            17123..17494
                     /locus_tag="IWO09_000731"
     CDS             17123..17494
                     /locus_tag="IWO09_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0263347.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IWO09_000732"
     CDS             17466..17825
                     /locus_tag="IWO09_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263348.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IWO09_000733"
     CDS             17997..19004
                     /locus_tag="IWO09_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0263349.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IWO09_000734"
     CDS             19029..20213
                     /locus_tag="IWO09_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0263350.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IWO09_000735"
     CDS             complement(20650..22233)
                     /locus_tag="IWO09_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0263351.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IWO09_000736"
     CDS             complement(22811..22954)
                     /locus_tag="IWO09_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0263352.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="IWO09_000737"
     CDS             complement(23176..23655)
                     /locus_tag="IWO09_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0263353.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="IWO09_000738"
     CDS             complement(23728..24012)
                     /locus_tag="IWO09_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263354.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="IWO09_000739"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO09_000739"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="IWO09_000740"
     CDS             complement(24356..25642)
                     /locus_tag="IWO09_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0263355.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="IWO09_000741"
     CDS             25780..26451
                     /locus_tag="IWO09_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0263356.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="IWO09_000742"
     CDS             26417..26905
                     /locus_tag="IWO09_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0263357.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="IWO09_000743"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="IWO09_000743"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0263358.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="IWO09_000744"
     CDS             27866..28630
                     /locus_tag="IWO09_000744"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0263359.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="IWO09_000745"
     CDS             28863..31076
                     /locus_tag="IWO09_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0263360.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="IWO09_000746"
     CDS             31087..31533
                     /locus_tag="IWO09_000746"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0263361.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="IWO09_000747"
     CDS             31704..32963
                     /locus_tag="IWO09_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0263362.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="IWO09_000748"
     CDS             complement(33052..33999)
                     /locus_tag="IWO09_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0263363.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="IWO09_000749"
     CDS             34393..35685
                     /locus_tag="IWO09_000749"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0263364.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="IWO09_000750"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="IWO09_000750"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0263365.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="IWO09_000751"
     CDS             37578..38453
                     /locus_tag="IWO09_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0263366.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="IWO09_000752"
     CDS             38719..40602
                     /locus_tag="IWO09_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0263367.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="IWO09_000753"
     CDS             complement(40677..40961)
                     /locus_tag="IWO09_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0263368.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="IWO09_000754"
     CDS             complement(40951..41685)
                     /locus_tag="IWO09_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0263369.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="IWO09_000755"
     CDS             41814..42437
                     /locus_tag="IWO09_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0263370.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="IWO09_000756"
     CDS             42629..43750
                     /locus_tag="IWO09_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0263371.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="IWO09_000757"
     CDS             43757..46885
                     /locus_tag="IWO09_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0263372.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="IWO09_000758"
     CDS             46904..50098
                     /locus_tag="IWO09_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0263373.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="IWO09_000759"
     CDS             complement(50244..50951)
                     /locus_tag="IWO09_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0263374.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51716..52114
                     /gene="spxA"
                     /locus_tag="IWO09_000760"
     CDS             51716..52114
                     /gene="spxA"
                     /locus_tag="IWO09_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0263375.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17114 a         9064 c        10787 g        15175 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgt agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca
    51361 gtattattta aaatgacaga aaacattact attaaagaaa taaaaaacat aaaaaatctt
    51421 aagaaatcgg tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt
    51481 catatgttta gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag
    51541 cgagaatgct ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt
    51601 atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta
    51661 ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt
    51721 aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga
    51781 acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt
    51841 aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa
    51901 agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact
    51961 tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca
    52021 agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga
    52081 attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga
//
LOCUS       DACECJ010000013        51634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.013, whole genome shotgun sequence.
ACCESSION   DACECJ010000013 DACECJ010000000
VERSION     DACECJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.013"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="IWO09_000761"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="IWO09_000761"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0263376.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="IWO09_000762"
     CDS             1281..1727
                     /locus_tag="IWO09_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0263377.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2000..3352
                     /locus_tag="IWO09_000763"
     CDS             2000..3352
                     /locus_tag="IWO09_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0263378.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3523..3810
                     /locus_tag="IWO09_000764"
     CDS             3523..3810
                     /locus_tag="IWO09_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263379.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4036..4479
                     /locus_tag="IWO09_000765"
     CDS             4036..4479
                     /locus_tag="IWO09_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0263380.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4485..5669)
                     /locus_tag="IWO09_000766"
     CDS             complement(4485..5669)
                     /locus_tag="IWO09_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0263381.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5937..6635
                     /locus_tag="IWO09_000767"
     CDS             5937..6635
                     /locus_tag="IWO09_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0263382.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6642..7319
                     /gene="nth"
                     /locus_tag="IWO09_000768"
     CDS             6642..7319
                     /gene="nth"
                     /locus_tag="IWO09_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0263383.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7445..7978)
                     /locus_tag="IWO09_000769"
     CDS             complement(7445..7978)
                     /locus_tag="IWO09_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263384.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8275..10656)
                     /locus_tag="IWO09_000770"
     CDS             complement(8275..10656)
                     /locus_tag="IWO09_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0263385.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWO09_000771"
     CDS             complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWO09_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0263386.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11357..11896
                     /locus_tag="IWO09_000772"
     CDS             11357..11896
                     /locus_tag="IWO09_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0263387.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11986..12405
                     /gene="gpsB"
                     /locus_tag="IWO09_000773"
     CDS             11986..12405
                     /gene="gpsB"
                     /locus_tag="IWO09_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0263388.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12427..12794
                     /gene="rnpB"
                     /locus_tag="IWO09_000774"
     ncRNA           12427..12794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO09_000774"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13003..14169
                     /locus_tag="IWO09_000775"
     CDS             13003..14169
                     /locus_tag="IWO09_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0263389.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14215..15711
                     /locus_tag="IWO09_000776"
     CDS             14215..15711
                     /locus_tag="IWO09_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0263390.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            15792..16475
                     /locus_tag="IWO09_000777"
     CDS             15792..16475
                     /locus_tag="IWO09_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0263391.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17120..18310
                     /locus_tag="IWO09_000778"
     CDS             17120..18310
                     /locus_tag="IWO09_000778"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0263392.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            18412..19365
                     /locus_tag="IWO09_000779"
     CDS             18412..19365
                     /locus_tag="IWO09_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0263393.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            19434..20783
                     /locus_tag="IWO09_000780"
     CDS             19434..20783
                     /locus_tag="IWO09_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0263394.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(20966..21289)
                     /locus_tag="IWO09_000781"
     CDS             complement(20966..21289)
                     /locus_tag="IWO09_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263395.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            21418..22581
                     /locus_tag="IWO09_000782"
     CDS             21418..22581
                     /locus_tag="IWO09_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263396.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            22651..24000
                     /locus_tag="IWO09_000783"
     CDS             22651..24000
                     /locus_tag="IWO09_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0263397.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24045..24476)
                     /locus_tag="IWO09_000784"
     CDS             complement(24045..24476)
                     /locus_tag="IWO09_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0263398.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            24864..27506
                     /gene="alaS"
                     /locus_tag="IWO09_000785"
     CDS             24864..27506
                     /gene="alaS"
                     /locus_tag="IWO09_000785"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0263399.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27706..28302
                     /locus_tag="IWO09_000786"
     CDS             27706..28302
                     /locus_tag="IWO09_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0263400.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            28424..29572
                     /locus_tag="IWO09_000787"
     CDS             28424..29572
                     /locus_tag="IWO09_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0263401.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(29610..30023)
                     /locus_tag="IWO09_000788"
     CDS             complement(29610..30023)
                     /locus_tag="IWO09_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0263402.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30102..30533
                     /locus_tag="IWO09_000789"
     CDS             30102..30533
                     /locus_tag="IWO09_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0263403.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(30632..30865)
                     /locus_tag="IWO09_000790"
     CDS             complement(30632..30865)
                     /locus_tag="IWO09_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0263404.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31048..32718
                     /locus_tag="IWO09_000791"
     CDS             31048..32718
                     /locus_tag="IWO09_000791"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0263405.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            32865..33251
                     /locus_tag="IWO09_000792"
     CDS             32865..33251
                     /locus_tag="IWO09_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263406.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            33238..33936
                     /locus_tag="IWO09_000793"
     CDS             33238..33936
                     /locus_tag="IWO09_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0263407.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            33941..34405
                     /locus_tag="IWO09_000794"
     CDS             33941..34405
                     /locus_tag="IWO09_000794"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0263408.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            34402..35313
                     /locus_tag="IWO09_000795"
     CDS             34402..35313
                     /locus_tag="IWO09_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0263409.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            35548..36087
                     /gene="pyrR"
                     /locus_tag="IWO09_000796"
     CDS             35548..36087
                     /gene="pyrR"
                     /locus_tag="IWO09_000796"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0263410.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36101..37384
                     /locus_tag="IWO09_000797"
     CDS             36101..37384
                     /locus_tag="IWO09_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0263411.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            37397..38323
                     /locus_tag="IWO09_000798"
     CDS             37397..38323
                     /locus_tag="IWO09_000798"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0263412.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            38342..39622
                     /locus_tag="IWO09_000799"
     CDS             38342..39622
                     /locus_tag="IWO09_000799"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0263413.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            39627..40703
                     /gene="carA"
                     /locus_tag="IWO09_000800"
     CDS             39627..40703
                     /gene="carA"
                     /locus_tag="IWO09_000800"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0263414.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            40708..43893
                     /gene="carB"
                     /locus_tag="IWO09_000801"
     CDS             40708..43893
                     /gene="carB"
                     /locus_tag="IWO09_000801"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0263415.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            43947..44723
                     /locus_tag="IWO09_000802"
     CDS             43947..44723
                     /locus_tag="IWO09_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0263416.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            44720..45652
                     /locus_tag="IWO09_000803"
     CDS             44720..45652
                     /locus_tag="IWO09_000803"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0263417.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            45649..46365
                     /gene="pyrF"
                     /locus_tag="IWO09_000804"
     CDS             45649..46365
                     /gene="pyrF"
                     /locus_tag="IWO09_000804"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0263418.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            46372..47061
                     /locus_tag="IWO09_000805"
     CDS             46372..47061
                     /locus_tag="IWO09_000805"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0263419.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            47034..47303
                     /locus_tag="IWO09_000806"
     CDS             47034..47303
                     /locus_tag="IWO09_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263420.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            47341..47982
                     /locus_tag="IWO09_000807"
     CDS             47341..47982
                     /locus_tag="IWO09_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0263421.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48013..48717
                     /locus_tag="IWO09_000808"
     CDS             48013..48717
                     /locus_tag="IWO09_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0263422.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            48714..49616
                     /locus_tag="IWO09_000809"
     CDS             48714..49616
                     /locus_tag="IWO09_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0263423.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(49774..51480)
                     /locus_tag="IWO09_000810"
     CDS             complement(49774..51480)
                     /locus_tag="IWO09_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0263424.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        16894 a         8983 c        11096 g        14661 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga atcttgtaga
     1861 tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt gaattcacaa
     1921 gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa ggacaaaatg
     1981 ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg caggtactgc
     2041 agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat atgaaagaaa
     2101 tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag tagtaaagga
     2161 cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag caacagttaa
     2221 aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg caaaagatct
     2281 gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa ctggttcatg
     2341 gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat gcaaaaacta
     2401 caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag taattgttgg
     2461 tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa aacaagtgac
     2521 attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat tcacagatat
     2581 cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa atgttactga
     2641 atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag atacatttga
     2701 tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga tcgaaggaaa
     2761 agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga caagtaatcc
     2821 agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg gcacgcaaaa
     2881 ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa ataacttgac
     2941 agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc tatttggctg
     3001 gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc tagaagtcca
     3061 agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg aaaaagtaat
     3121 gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt tcatgtccaa
     3181 atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata aaatgactgt
     3241 agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc catggaatta
     3301 cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat aaagaaaaag
     3361 ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta caattagttg
     3421 tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac ataaaagaac
     3481 aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa agaatatttt
     3541 gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt aacgatcaat
     3601 attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga ggaatattac
     3661 acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt cagtagacgc
     3721 atctactcag atgaaaatga cggcacattg aatacagagt acacatatga aggtcagcca
     3781 tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc gctcatcctt
     3841 aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat cttttaatca
     3901 gaaaaagggg ttatgacaag ctttgtcaac cccttttttt cttggtttca gattgggttt
     3961 tcctacaaaa tatttttatc tttgttgaat aggaaacaaa aaaacaatat aatgaaagaa
     4021 aaagaaggtg tagaaatgga taaaagacaa ctgctaatag aagaatatct ggatttagct
     4081 aatgagatta ttcatcgtaa taaaccagaa atggaaaaag aatttgcagg atttactcta
     4141 aatgaactag aagtgattga acatatcggg aaaatccctg atcctaacgt gacaaaatta
     4201 gcgaatgcta gttacatgac tagaggagcc atcagtaagc taacaaaaaa actgatagct
     4261 agagggatca taaacactta tcaaagcgaa gagaataaaa aagaaatcta ttttcaattg
     4321 acgccaatcg gacaaaaaat aaggaataga cacgagcaac tccatcaaaa atgggtacag
     4381 cgagatgcta ctgtatttga aaatatgtca aaagaagaat ttgacacagt atttcgtttt
     4441 atcggtcgat acagagaatt tatacgaagt agcaaatgaa gctactattt tttattgtag
     4501 tgataatagg caaaaatcat aagcaaaaat ccgaataccg tcggaagaaa tggtgctgca
     4561 gcataaagct gtcctgcgat tagcgggccg atgacgcgcg atagagattg tatactttgg
     4621 gtaccgccca ttacaagacc ttgctggtta tctgagacag aattggatag ttggccattg
     4681 aatacaggag taaaaataga atcaccgaaa ccataacaga taatcccaaa taaaaacaaa
     4741 atcggcagta gaagaattcc agataatgtg atgaatagat aggcgaggat ttcactgaac
     4801 atccctattc ttgtgatctg ctgttctgaa aattttttga gcaatcgagg cataatgaac
     4861 aattgcgaga cagtatccaa gattccaatc aaagagaagc ttaaaccaac caacccagcc
     4921 ttccactgaa aggcgtcaat cgaaaattga gaaaagatag attgaagaga gccagctgcc
     4981 aaccaaacag caaaacctgc tgtcaataaa ggaataacgg atgaaatctt gaataattgt
     5041 tgtaattggt gaaaaggtct tacatgggag aaacttaaat ctactgaacg tttttttatt
     5101 ggaaggcttt ctagcataaa tgtgtagcca taaacggcat ttaagaaagt aaagagtgct
     5161 ccaataaata taggaacaga attccccaac tcagctaata gaccccctat aataggaccc
     5221 agcgttgttc ctataccgac taaagcaccc atccatccaa aaactttggt tctttcatta
     5281 ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga tttctcctgc cgtcaatcct
     5341 tcaatgatac gaccaaggaa caacatccat atcgaatttc ctaaaccaaa aagcaaatat
     5401 ccgatacttg atcctaataa actgataata aggatcggat gacgcccaaa atgatcactt
     5461 aagctaccga gaatgggggc agataagaag gcagctagag cataaacaga catgagcaaa
     5521 gtgatataga aagcttgctg atgtatggag tgagaataag gcagcgttaa aaacggaagt
     5581 acaggactga cgatagtaaa tcctaatcca gtgaataaga cagagacgta tccaaaaact
     5641 ttagaatgtt tatttgtgaa actgtacatg taaaaactcc tttatgcaat aatgttaact
     5701 tggaaacaaa aatagtgtat agtaattttg tttccttgtc aacattctaa ggaacagcga
     5761 aatctactta atttatttat tccgaaaagt gaagatacgc atcaaaaaca tgaacaaaca
     5821 aaaatcaagt agacgacatt gtaaatctga ccgatcagtg aagtaaatag aaaagaaacc
     5881 tgttcttgct tcccatgttt tttttacgta tactgtgatt gagcggagga gaaatcatgt
     5941 tgaatttaga aaattactta agcgctggac aaacagtcat atctaatctt ttacttaaaa
     6001 attatcatag gatcggtcta caatctgaag aatttttatt tgttctacag ttgcatatgg
     6061 cacagcttga aggagatact ttccctgatt tgcagatgat tgctatggat atgggaatca
     6121 aacaagatca gatttttcaa atactggatc gtctcgtaac aaatggattt atcaagatag
     6181 aaacgacgat tgtcaacggt aaaaaagcag atcgatataa tctttacccc atctatgatc
     6241 tattaggtga gtatttaaaa acacaagaaa aaaaatacga agaaaaaaca caagaaaaag
     6301 aaatccagtc agtgtatcaa ttatttgaac aagaatttgg acgcccgctt tcttcgatcg
     6361 agttccagcg gatcggacaa tggctagaag aagaccatta tcagccggaa attttgaagc
     6421 tagcactgcg ggaagccgta ttaaatcagg cttatagttt taattacgtt gaccgcattt
     6481 tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca agtggaagaa gaccaaaaac
     6541 gaagaaaaca gcagcttctt caaagagaag tagaacagtc agctcaacaa gaagaattgc
     6601 cgaaagtctc actgaaaaat tggttggagg agtagcggaa tatgttaagc aaacaaaaaa
     6661 caatggaagc tctagaaacg atgtatggaa tgtttcccga agcacatgga gaactcaagc
     6721 acaataatcc atttgagtta ttgattgcag tgatcttaag tgcccaggcg acagatgtat
     6781 cagtgaataa agcaacgcca gatttgtttg cctcttttcc gacacccgat gcgttagcag
     6841 aagcttcaat cgatgaaatc attcttaaaa taaaaacgat tggtttgtat cgaaataaag
     6901 caaaaaacat caaagcgtgt gcacagcagc tgattgaacg tttcgatggg caagtgccaa
     6961 cgtcaagaga agaattgatg tctcttcctg gtgtaggcag aaagacagca aatgtcgtat
     7021 tgggggatgc attcgggata ccagcaatcg cagtagatac ccacgtggaa cgtgtctcaa
     7081 aacgcctgag gatctgcaaa ctagatgcta cggtcatgga agtagaagaa acactgatgc
     7141 ggaaagtacc acaagaatta tgggtaaaga cacatcatac actgattttt tttggtagat
     7201 atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc tttgctatca atatgtcaag
     7261 atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa aaagaaacca cgcctgtaga
     7321 cgtggtttct ttaattgcaa ataaatgtat gaattatctt gttattatgg agaaaagctg
     7381 tttcgtttat cttttttgca tgaaaaagta aatgtacata ctcagtagct aggatggtaa
     7441 cttattacaa tttattgcga taaaaataaa agttgttatt tttattggga aaatacccga
     7501 tgtagaaaat aatgccttcc aatataccta taaaataagt ccagtattcc aaaggattat
     7561 ataaataagt gacgtatcta tctaaaggtg taataaaaat gataagtgat aataaaaaaa
     7621 taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc atcagaaaaa
     7681 tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga gagcttcttt
     7741 ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata aatgcaagga
     7801 taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct tttttattct
     7861 tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt atgataatta
     7921 agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc aaaaccatat
     7981 attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact aaataaaaga
     8041 ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta tatgtaatga
     8101 taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct
     8161 ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt
     8221 tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct
     8281 gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct
     8341 agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc
     8401 ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct
     8461 ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt
     8521 tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga
     8581 tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac
     8641 acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg
     8701 cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat
     8761 ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag
     8821 gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt
     8881 ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc
     8941 ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc
     9001 tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac
     9061 atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt
     9121 taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt
     9181 cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc
     9241 tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg
     9301 gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc
     9361 ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa
     9421 agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac
     9481 tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt
     9541 tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta
     9601 agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa
     9661 attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc
     9721 attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag
     9781 ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat
     9841 tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg
     9901 ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg
     9961 cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc
    10021 gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga
    10081 ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg
    10141 atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc
    10201 gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc
    10261 aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt
    10321 tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag
    10381 ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa
    10441 ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca
    10501 gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga
    10561 ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt
    10621 ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata
    10681 atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt
    10741 tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct
    10801 tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag
    10861 acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt
    10921 cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg
    10981 cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg
    11041 taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt
    11101 ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac
    11161 tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc
    11221 ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat
    11281 agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa
    11341 agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt
    11401 cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa
    11461 aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt
    11521 gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact
    11581 atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca
    11641 agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc
    11701 ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg
    11761 ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc
    11821 acagaattac caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca
    11881 aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa
    11941 aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt
    12001 tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc
    12061 gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta cagcaaagaa
    12121 ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa
    12181 actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg
    12241 accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag
    12301 cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat
    12361 gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa
    12421 tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc
    12481 acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg
    12541 gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc
    12601 acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag
    12661 caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt
    12721 ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa
    12781 catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta
    12841 taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat
    12901 tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga
    12961 ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag
    13021 ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga
    13081 gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa
    13141 acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg
    13201 gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa
    13261 gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta
    13321 tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc
    13381 tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa
    13441 gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc
    13501 aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca
    13561 cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca
    13621 ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt
    13681 gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca
    13741 gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat
    13801 ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt
    13861 acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg
    13921 gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa
    13981 gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac
    14041 ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt
    14101 gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa
    14161 gacgaataat cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa
    14221 gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc
    14281 ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta
    14341 gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc
    14401 attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc
    14461 aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat
    14521 acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt
    14581 tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac
    14641 tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg
    14701 actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc
    14761 cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg
    14821 aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt
    14881 cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca
    14941 cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac
    15001 gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt
    15061 tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt
    15121 ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga
    15181 acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt
    15241 ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa
    15301 atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat
    15361 caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa
    15421 gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg
    15481 tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg
    15541 tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca
    15601 tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac
    15661 ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt
    15721 tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag
    15781 aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc
    15841 atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa
    15901 ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg
    15961 atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat
    16021 tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa
    16081 atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg
    16141 ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt
    16201 tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg
    16261 acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct
    16321 tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct
    16381 tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta
    16441 ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag
    16501 ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg tttaccaaga
    16561 ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc
    16621 gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg
    16681 aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca acaagtaaaa
    16741 cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag atggaaattt
    16801 tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac aattgatttt
    16861 aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag agtaaaatag
    16921 atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta aaaagatact
    16981 gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat caaaaatata
    17041 gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg caagacagat
    17101 gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag agaaagaatt
    17161 ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa gtgaaaatgg
    17221 tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca attatcttgg
    17281 atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa cttatggtgt
    17341 aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc aattagaaga
    17401 aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg gattcaactg
    17461 caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat cggatgaatt
    17521 aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca tccgaattaa
    17581 acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa gtaagaaata
    17641 taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca ttgcacgatt
    17701 gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg atgatgcgca
    17761 cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat taagcgacaa
    17821 aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg gttatgtagc
    17881 tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc tattttctac
    17941 ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga tgcaagaaca
    18001 tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag aattgaaaaa
    18061 agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt tgggagacga
    18121 aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg ctaaaccaat
    18181 cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga ctgcggaaca
    18241 tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa aagaaatgga
    18301 aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat gttcacgcat
    18361 atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat catgaaaaaa
    18421 gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg gctacgtcaa
    18481 gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt cattgagaaa
    18541 aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag catagcagaa
    18601 caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt tgcagaagca
    18661 aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt ggaagtcgct
    18721 agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg tccaaatact
    18781 ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta tggggtaacg
    18841 aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt agatactcgt
    18901 ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg tacgacagac
    18961 tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag tttcattgat
    19021 caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat tataaaactc
    19081 atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc ggcgatgtct
    19141 tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt tgaaatggat
    19201 tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc actagatgtt
    19261 tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt gatgacaaag
    19321 gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa gaaccacatc
    19381 caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa gggatggaag
    19441 aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa cgtacgatgg
    19501 gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg gtatttttta
    19561 aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt tcttggtttc
    19621 tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca gctgctattc
    19681 cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca gcagcttttt
    19741 tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc ttgtcgatca
    19801 ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt gttcccgtag
    19861 ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa gctggagggg
    19921 cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc gtcattttcg
    19981 gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca ggagaaaatc
    20041 tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca tacgacggtt
    20101 ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat ttgccaaaag
    20161 caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat gcggtgttct
    20221 taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt gacgttgcaa
    20281 aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg atttctgttt
    20341 atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt atggcaaaag
    20401 aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact aaagttcctg
    20461 tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc ggtggttttg
    20521 atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg gtctttgttg
    20581 gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa gttccaatgt
    20641 atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca agcacgctta
    20701 tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt atcccgattt
    20761 atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact gcacttctct
    20821 gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa aagtactcgt
    20881 taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta aaagacagaa
    20941 atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact attcggcgta
    21001 cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga agccaaatca
    21061 gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta tccatttcaa
    21121 gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa ataatcagga
    21181 gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc gtagcataaa
    21241 ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc tcgacacccc
    21301 ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc gttacttgct
    21361 tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag gagtcatatg
    21421 gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt agccttagcg
    21481 atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct agtaacaagg
    21541 gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt ggtgatctta
    21601 ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga gctgcattta
    21661 tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga acctagtacc
    21721 tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt tttattactc
    21781 ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa aacgatgatt
    21841 gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact ttattttatc
    21901 tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac attgggtgga
    21961 atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag aagacgaatg
    22021 gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt ggtcgcttat
    22081 ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt ctttaaaatt
    22141 gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga agtaggggta
    22201 tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa aattgtgcgt
    22261 atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat gatccgctac
    22321 gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct tcagcaaatc
    22381 atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc tctcggtgtc
    22441 ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag aaaaaaacgg
    22501 attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg ccaaacaaag
    22561 gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata atgccaaagt
    22621 cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt ccgcttcaaa
    22681 ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga ggtccaggaa
    22741 cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca aactggtaca
    22801 ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga aaaagaggaa
    22861 gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta tgaagcagct
    22921 acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt cggcggtaca
    22981 gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt agtaggcacg
    23041 cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac cgcatttgca
    23101 tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga agtagaccaa
    23161 attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac tattccagaa
    23221 aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga aatcaaacca
    23281 aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg taaagatgag
    23341 aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat cgtatttgcc
    23401 aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg cttgaaagta
    23461 gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg acaagtacag
    23521 aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat cgatatcgaa
    23581 ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt tatccatcgt
    23641 gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata ttcgccaaat
    23701 gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc aaaagaaatc
    23761 aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg agaaaaatca
    23821 aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa aaagatcaag
    23881 cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa agaacgcaaa
    23941 atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc acataaataa
    24001 aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc tagacttttt
    24061 ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt atgataccct
    24121 tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt ctgacaacct
    24181 tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc ttttcctcgg
    24241 tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt cttttcataa
    24301 cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg ttgcgggata
    24361 tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa aacttgatat
    24421 ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt tttcatcata
    24481 ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc gtaccaaaaa
    24541 tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca gtgatcaata
    24601 gaatgcgtat actaattaag tacgaaaagg atatgatttg ttttttaaga ctcacagaaa
    24661 attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt ttctattttg
    24721 gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt acacatcatt
    24781 gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt tttgtattta
    24841 atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt cgacaaatgt
    24901 ttcttgattt cttccaatct aaaggacata cgattgaacc tagtgcgtct ttgatccccg
    24961 tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa aaatattttg
    25021 atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct attcgaacaa
    25081 atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag atgctgggta
    25141 atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg gaatttttga
    25201 ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt tatccaaaag
    25261 atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat cacattattg
    25321 atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat acggaaattt
    25381 tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa aactatccag
    25441 gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa ttcaaccata
    25501 agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg atgggattgg
    25561 aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac ttgtttatgc
    25621 ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa aacccagtaa
    25681 cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt gctattggcg
    25741 acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg cttcgtcgtg
    25801 ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt ttagtccctg
    25861 ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa gaatttattg
    25921 aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa ggtttggaaa
    25981 tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta gcaggaaaag
    26041 acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa gaggtggcag
    26101 aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat gcacaacgcg
    26161 aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct gctttattga
    26221 cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca gatagtcaat
    26281 tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt gaagctcaaa
    26341 tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg gatcaaggat
    26401 ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa aaagcgccta
    26461 atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga caaacctatc
    26521 atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca gcaacccatc
    26581 tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct ggttctttag
    26641 tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact tcagatgaat
    26701 tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca gttgaaacaa
    26761 ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt ggtgaaaaat
    26821 acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg tgcggaggca
    26881 cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa tcaggaattg
    26941 gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa ctacttgaag
    27001 aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa ttgaaagaag
    27061 ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag gaaaatgaac
    27121 aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat ccagaaacga
    27181 tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat cagctgcgtc
    27241 aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta gcaaacgaac
    27301 aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa gctggactaa
    27361 aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt ggtggacgtc
    27421 cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca cttaaagctg
    27481 cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac aaaagttcaa
    27541 acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct catacttttt
    27601 tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag ttgacgaaag
    27661 tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa tagtagaaat
    27721 aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt acaagcgatg
    27781 gttccttggc tggggtttat tccattagga ttcattagtt taacaattat ccatatcaca
    27841 gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg cttgttttgg
    27901 ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga tacattagtg
    27961 ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt agcaggcttg
    28021 gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc ctttgcaggg
    28081 gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt attttataca
    28141 ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac cttagcaggg
    28201 gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac accattacta
    28261 gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc ttttaatagt
    28321 aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc gtcctctcca
    28381 tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta agaaacaaga
    28441 agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag cgggtatctt
    28501 aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc ttttttcagg
    28561 aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt catccatatt
    28621 gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc cttatggaca
    28681 cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt atgtaggatt
    28741 tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct ctttaacacc
    28801 catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag gcaaaatgta
    28861 tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa cagatagtta
    28921 taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat gggtaactgg
    28981 atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt atagtggatt
    29041 gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc cagcagagat
    29101 tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc atgatttatt
    29161 ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag tagatgaatc
    29221 aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact ttaaaaagtc
    29281 tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg aaaaatacta
    29341 taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc ttaatattca
    29401 tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg tccctgagaa
    29461 attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag aagaaaaaat
    29521 cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt aaatgagaaa
    29581 ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt taattgttta
    29641 tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg caaccattgc
    29701 aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta gggaaggaag
    29761 agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct gtctgaaaaa
    29821 cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt ttttaaaaag
    29881 acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag ctgttcttgt
    29941 ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc ttttgtagag
    30001 gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa ctaatgttat
    30061 ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa aatattcgat
    30121 ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc attctgtttt
    30181 ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat tttgttttag
    30241 gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta aaatcaaact
    30301 ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc ggacagattc
    30361 gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag aaagccccat
    30421 ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt gaaaaaaacg
    30481 gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc taattttaaa
    30541 aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac gtacggttca
    30601 tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta tcggtagtct
    30661 gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg atgatctcaa
    30721 attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc gcagtaaaat
    30781 gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt ttgttgtcaa
    30841 accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct gatcatagcc
    30901 ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta attttatgtt
    30961 ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat attttgttga
    31021 ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc acaagaaaca
    31081 gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga cgatttggaa
    31141 ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc aaaggataac
    31201 gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg cgaaggaaag
    31261 tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa atctgttatt
    31321 gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc tgcaggcgga
    31381 ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg tgacatgcat
    31441 gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat tcatcaagga
    31501 aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga tctcaatgat
    31561 cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt tccaagagaa
    31621 gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct tgcggcagac
    31681 cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt tgaccgtcag
    31741 cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct gaaagatgca
    31801 atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca cgggggaccg
    31861 tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc attgagatta
    31921 gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga gaaattttta
    31981 gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt cgccactgtc
    32041 cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga gaatcttgat
    32101 gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga acaatatcag
    32161 ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga actcacactg
    32221 ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt atgggcagat
    32281 ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga tagaaaagaa
    32341 gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac agaaacaatc
    32401 gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca aaaacaaata
    32461 gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa aacacagtat
    32521 tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat cacgatccgc
    32581 gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga tgttatgacg
    32641 atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa tgacggagaa
    32701 gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt attttgacta
    32761 cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc attctttttc
    32821 tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa acgacattta
    32881 taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc aacaatgaaa
    32941 aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg ccctttacga
    33001 tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa gcaaagcctg
    33061 cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta tttctagcaa
    33121 ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca gttattgtgt
    33181 tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag gaaaattatg
    33241 gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat gagagacgag
    33301 atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc tcaaagaaaa
    33361 caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt aaggaatgcg
    33421 atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga ttggacagtc
    33481 aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct gcctcgattt
    33541 gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt aaagatagta
    33601 gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt agcattgatt
    33661 actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc ggttgattta
    33721 acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa ttatcatttt
    33781 gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca agggactttg
    33841 gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg gatcgtacga
    33901 cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta tttattggat
    33961 attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca ctgataatct
    34021 agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc atattcgaaa
    34081 cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta tagtgacact
    34141 cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag gtaatcgctg
    34201 gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta tcgaccgtat
    34261 tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc cgatttttaa
    34321 cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata ttattttaga
    34381 tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca tgaatctggt
    34441 aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca aattcagctg
    34501 tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa ttacaaagta
    34561 aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga tgtggtccca
    34621 gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat aaacaaacct
    34681 caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt caatgcgctg
    34741 atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg tattgttcac
    34801 cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca ggcacacgaa
    34861 tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc tttagtccat
    34921 ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc gaaggaaaac
    34981 cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt tactgtattg
    35041 gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag aacacaccag
    35101 atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt atacggaccg
    35161 aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg ttttacgcac
    35221 ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt tgaaaaaact
    35281 ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag taattaacaa
    35341 cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct aagaagggtg
    35401 ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag tatagctatc
    35461 agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc aggagttttt
    35521 ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga tcaggtaacc
    35581 atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca tagtatccaa
    35641 gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag aattgccgaa
    35701 cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat cactttatac
    35761 cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga catcccagtt
    35821 tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg acgaacgatt
    35881 cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc attagcagtc
    35941 ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg gaagaacatt
    36001 ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg acaagatcgc
    36061 atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga aaaaaggatt
    36121 tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac attgggtagg
    36181 attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc ccatcttagt
    36241 aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg tgtacttatt
    36301 tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca ttgcagcgat
    36361 gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag tcacaactgg
    36421 attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat ggctggataa
    36481 aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg gacttgcggc
    36541 aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt atattgctgt
    36601 agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag gttttctcgg
    36661 attgattccg attttactag gtattattag cggatacatc gtagccttgc tgtttggaat
    36721 cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact ttgagattcc
    36781 ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg cacccatcgc
    36841 ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac taactaaacg
    36901 taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat tagcacaaat
    36961 cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca ttggtgttct
    37021 ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat ttgcagtcat
    37081 tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac cagtcatctc
    37141 aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga ttttaatcga
    37201 gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta tcttagtagt
    37261 aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa tggcattagc
    37321 aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa gcgaagaaaa
    37381 ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc atttattgac
    37441 cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc aggaattcaa
    37501 gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc tctttttcga
    37561 aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag ggctagaagt
    37621 gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat acgatacagt
    37681 actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg atgagaacta
    37741 ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg gaggagacgg
    37801 aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg aagaatttgg
    37861 tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc gcgtagctaa
    37921 atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag gtcctagaga
    37981 atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg aactactaga
    38041 tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg gaaaagaaag
    38101 tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg cgaagaagtt
    38161 gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag aattagctga
    38221 ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg gagtattcgt
    38281 cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg aggatgaaaa
    38341 aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga cgacaccagc
    38401 agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct cagaagccga
    38461 atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag tggacgtcca
    38521 tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg ggactcgctc
    38581 agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc ctgtacctga
    38641 tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag ttgtgaaggt
    38701 attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg atcaagaagc
    38761 actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc aaacagctgg
    38821 gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag tagcccatac
    38881 agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg cagaagaact
    38941 tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag atctgttgct
    39001 agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag aaagtgtccg
    39061 tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt caccccatca
    39121 tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa tgaacccgcc
    39181 gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg gtacgatcga
    39241 ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa gtttcatgaa
    39301 atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata ctcattttgt
    39361 agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta aaccggctga
    39421 gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg tagcagtttt
    39481 tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag gggagaatac
    39541 accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca acggaaaact
    39601 tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa gatggaacag
    39661 ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc gtttttacaa
    39721 caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt caaatcatta
    39781 catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat gaatcgattt
    39841 ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca aactggcgaa
    39901 accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata tcaggtatcg
    39961 atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct agtatagttg
    40021 atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt ctagcaacca
    40081 atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca ggtcggaaag
    40141 tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt aaaagaaact
    40201 gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg ctatcaccag
    40261 acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa gcaatcgaaa
    40321 tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt caccagttat
    40381 ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga ggattaaatc
    40441 acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat catggctatg
    40501 cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta gaagtaaatg
    40561 acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg cagtttcatc
    40621 cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt atggaaatga
    40681 tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa gaaaattatg
    40741 gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta cgcaggaaca
    40801 caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa ttctaatcct
    40861 gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc gatcacactt
    40921 gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc aactttaggc
    40981 ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt agaagaattg
    41041 aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga tcgtgatcta
    41101 ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat cgtcaataca
    41161 gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt tcgtccagcc
    41221 tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt acgtatcgta
    41281 tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg aagtattgca
    41341 ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc aatcgttgtc
    41401 tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat cgtatttgcc
    41461 cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc tctttcgatt
    41521 attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga tccacatagt
    41581 tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc gttagcaagt
    41641 aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg attaacatta
    41701 gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc tgcattagat
    41761 tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg agaacggctt
    41821 ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa tatcgaagag
    41881 tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga acttcctgag
    41941 ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca agatgatcgc
    42001 ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt agcggatttg
    42061 acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat cgaacagcag
    42121 ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa cggttttaca
    42181 gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca gatgagaaaa
    42241 gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga attcgaatca
    42301 aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg atcagaaaag
    42361 ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt tgaatttgac
    42421 tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc aatcatcatg
    42481 aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt atattttgag
    42541 ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat aggagtaatc
    42601 gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc tgctggtgta
    42661 aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga tttattcgag
    42721 caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac aagtaaagaa
    42781 gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc aagctatgta
    42841 ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga ttacatggaa
    42901 catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt gcttggtggt
    42961 gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg gatcatggag
    43021 catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc acctcaaaca
    43081 ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc attaggtttg
    43141 aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt ttatgtgatc
    43201 gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac aggaattcca
    43261 atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact tggttatgaa
    43321 aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt ttcttttacg
    43381 aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg agaagtaatg
    43441 ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc tggattacac
    43501 ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga agaagcacta
    43561 gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa acatacagct
    43621 gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc tgaggaagca
    43681 gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt tgtcaatacg
    43741 attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag agaagcagtt
    43801 gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt tcgagtgatg
    43861 gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa gacaaaaaga
    43921 cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc gttcagcaaa
    43981 aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta aaagaaatgc
    44041 agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta ttacgccgac
    44101 cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt tatcgggtgg
    44161 aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta gatgtcatgg
    44221 gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa gtatttataa
    44281 tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta gtcgaaaaag
    44341 gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat tatgaaaaag
    44401 aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac ggtatgcacg
    44461 gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt tttgcatgtg
    44521 gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa aatgtccagt
    44581 tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc gtttgtcata
    44641 cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga ccgattttta
    44701 aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc ctggcctaga
    44761 attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag aatacggaaa
    44821 atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc caaatgcccg
    44881 ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga atgcaattgg
    44941 tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat tagaggagta
    45001 caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact atgtagaagt
    45061 ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca tttcttgccc
    45121 aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt ttcaattaac
    45181 acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc caaatgtaac
    45241 agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt ttacgatgat
    45301 caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt tagccaatca
    45361 aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga ttcaccaagt
    45421 agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag tggatgatgt
    45481 tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga atttcaccga
    45541 tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg aactaggcat
    45601 cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat gaatcaacta
    45661 gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga agactttctt
    45721 cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt gttctaccaa
    45781 gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt tttagatctg
    45841 aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc aaagcttggt
    45901 gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc ggcaatgcga
    45961 ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc tatcactcag
    46021 cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt ttctttagaa
    46081 aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg tgtggtttct
    46141 tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt ttgcttaact
    46201 ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt cacaccttct
    46261 caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac tcagtcaact
    46321 aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa aatgaatgtt
    46381 gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa tccaagtaat
    46441 ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg aatcacgatg
    46501 agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat caaagaagct
    46561 taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca tgccgcatgg
    46621 gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa agatcatggg
    46681 aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt gatcgaggat
    46741 ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag agaaggtgcg
    46801 gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa agcaaatttc
    46861 gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat tgaagcagct
    46921 ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg gaaaaaagat
    46981 cctgaaaatt ggcagattga agcgcgcttg atgaagatag agaaatccga tttgtgtctc
    47041 cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc atcttttcat
    47101 caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa ttcatttttc
    47161 taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt atatatagat
    47221 tttctctatt ttttggtaag ggagctgttt caaaaatggt aaagagcgga aatgttgcga
    47281 atatcagtta tttaatgata tgatattcgg aaaaagaaat ctcaaaggga ggtaactttt
    47341 atggggttga atgaatttat tttaggattg aacaatttag agacgatcac aagggcacct
    47401 ggatttttta aatatacaga gcatacagtt gcagcacatt cgtatcgtgt agcatctatt
    47461 gcgcaagttc taggagacat tgaagaagcg aatggacaac cgatcgattg gaaactgctc
    47521 tatgaaaaat cattgaatca tgactacaca gagcgtttta ttggtgatat caaaacgccg
    47581 gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa cagttgaaga aaagatgacg
    47641 gacgaattca tcaaccaaga gattccagct gagttccaag aagtttatcg ccgtcggttg
    47701 ttcgaaggaa aagatgaaac cgtagaagga gaactattag ccatttcaga taaggttgac
    47761 cttctttatg aatcttttga tgaaatcatc aaaaataacc cggaaaaagt ttataaggag
    47821 atgttcattg aggcagtgac tacgattaaa agctttgaac atcggccttc tgttcagtat
    47881 ttctttaaag agatatttcc ggaactattg aaccaggaat tttatggaaa agaggcgttt
    47941 ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata tgagatgcag
    48001 ggggaagtat agttgaaaaa gaatatggat gtcgaatggg gctattctgg agacaaaggt
    48061 ccggaaaatt gggcgagttt atgtgattgg tttgcgagag gtgcggaatt tccattgcag
    48121 tcgcctattc atttgaccaa gggggaagaa accaaaatag aaggagacac gctttccttt
    48181 cattatcaaa agcaacggtt tactgacaag gaattcaaaa acacgattca tttagtacct
    48241 tttgaccaac tcagttatgt agagttcaag aaagaacgct attatttgac agatattcat
    48301 ttccacttgc cttctgaaca tatcatcaac ggaaaacagg aagatattga atttcatttt
    48361 gttcatatga acagcaaaaa agagaatcta gttgtgggtg tcatgtacca gctaatggta
    48421 caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga aaaacatgag
    48481 cattgggtaa atccggatgt gttctttccg gagcaaaaaa gccatttcca ttatattggc
    48541 tcattgacaa ctccgcctac cagcgggcca atccaatggt ttgtaatgga tcatgtagga
    48601 aaactgaacc aagagtttct tctccccttc gatggtcagt atgctcgacc gaacaaccga
    48661 ccaatccagc ccttgaatgg aagagaaatt tattatttcg aggagtttga gcaatgaata
    48721 ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga gaagcggcaa
    48781 agaaaatgtt tgtctcccag ccaagtcttt cagctggtat caaagaatta gaaaccgagt
    48841 tagggatcac gctgtttact cgaacgaatc gaggggcata tttaacagaa gaagggcaag
    48901 acttcttaaa tcgtgccgag aaaatattgg tccagcttga tacattagaa actcactatc
    48961 taacgagtga aaagagagac aatttttcga tcgctgccca gcattatgat ttcttaggtc
    49021 ctttgaccgc acagctactc gaggaattca aagacgagat caaacaattt cggatcatgg
    49081 agacgacaac agcgagagtg atcgaagaag tcaaagagca gcacagtgag atcggcatta
    49141 tttatatgaa tgagcataat caagctggta tcagaagata tttacaacaa ggagagctaa
    49201 gtggccgcag cattggaaaa ttcaaaacgc atattttttt aaggcaagga catccattag
    49261 ctgaacacgc agagatcacc aaagaagaat tgttatctta tcctcaagtt cgttttacac
    49321 aggatggaaa taatttcccg tatttttatg aagaccttat cgagatccct gatcaagaaa
    49381 cagtgattta tacgagtgat cgaggtactt tgatgaatat cgttcttgaa actgatgctt
    49441 acgcttcagg atctggtatt gtgattggag ggattcgcga gcacttgcgg ttaatccctt
    49501 tggcagaagg cgaactaaat gaattttata tcatttattc ggcgaaacgt ccgctcagtg
    49561 aaatcgctca aagatttatc aaagagctta ctcggttact ggaccagcag agctaagaaa
    49621 agcagatgag aacgctgttt attgaacacc gttgatcaaa aaagaggggt tgtgacagaa
    49681 ttttgtcaca actcctcttt ttgtagcaaa caatttataa ctaattgctg ttgtgggaaa
    49741 gtacgcaaat atgattctag aaacactcat tgattacctg ttttgtttca tagctgtaat
    49801 tttttcttct tctggtgtca cgataatcgt cttttggttt tcataaataa cataaccagg
    49861 cttggcgcca tttggttttc ggatatgctt tacctgtaca agatcaaccg gcacttgggc
    49921 agagtatcgg tattttgaaa aataggcagc taactcggca gcttctgtaa ttgtttcatc
    49981 tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt atattttttg catgaagcca
    50041 atagtcggtt tttttagctg tcttcattgt cagctggtca ttttgaagat tgtttttacc
    50101 aacaagaatg agcgttccgt cagaagaaaa gtattgatca ggctgagatg gtttcttacg
    50161 tttttgtttt tttaagttct tctttttcag gtatccttct gaagtcaatt cttctttaat
    50221 tgcttcgata tccattgggc cggcaatttc cagttgagat aagacagatt caagatattg
    50281 gatttcatct ttggcttctt ggatttgttc gccaatcagc ttaacagcgt tcttcagctt
    50341 ttggtatcga tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg gatctagtgc
    50401 tattttgatt ggccgatctt cttcataata attaggtaaa acaacttctt ttgcgccgcg
    50461 tggtacttgt gtcagaaaag tagtaagtag ttcgccattt ctacggtaat tctcagcgtt
    50521 ttcggattct ttgagtgttt gttcacgttt tttcaattta tttttatttc gttttagttc
    50581 attttcgatt tttcgaatca gttctccgcc ttgttgtttt gcccgatctt tctctgcttt
    50641 ttcgtgataa tagctatcta ataattgact gagtgttgga taagctgtta cagcagatgc
    50701 ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt ccgtttcgta
    50761 aaaagtagga ataggctgat ggctgatggc tgagaaaaat tcgtcccata caaccatctt
    50821 ttcattagga cgttccagta atcttttggt cagctcttga gcgatatcga aaccgattcc
    50881 ctgaaactga cgttggattg cttttggatc tagttcagtt tgggaaagcc gctgaaagat
    50941 tttttctttc tcggagctaa atgggttcaa gacctcttgt tttggcggag caacatattc
    51001 tacaccagga agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt tgatcgcatc
    51061 cagtattttt ccagtttctc gattaactaa gacgatcgta ctatgacgtc ccattaattc
    51121 aacgatcaat atgatatttt gtagatcacc caactcgttt cgtttagcaa agtggaaatg
    51181 gatcactcga tcattttcga tttgttcgat gtcttctaaa atagcgccat ccaaatattt
    51241 tcgaagcatc atcacgaagt taggcggatt atcaggattc tgataatcga tttgggtaat
    51301 ctgtacacga gcataacttg ggtgcgcaga taataataat cgatggtttt tgccgcgtga
    51361 acgaatcacc agcacgattt cattttcata aggctgatgg attttcgaga tccggccaga
    51421 tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta aatacaccat caaaggacat
    51481 ggttcattcc tcatttcttt tcataagtat attcattata acgaaaaaaa gaagaaaaac
    51541 caatttggcc gaatagtaaa gcaaaagtct agaaaaatct tggactaatt acataaaaaa
    51601 cagtgtttct atcgttgaaa tccaaatttt tagg
//
LOCUS       DACECJ010000014        48702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.014, whole genome shotgun sequence.
ACCESSION   DACECJ010000014 DACECJ010000000
VERSION     DACECJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.014"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWO09_000811"
     CDS             23..646
                     /locus_tag="IWO09_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263425.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWO09_000812"
     CDS             1096..2145
                     /locus_tag="IWO09_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0263426.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWO09_000813"
     CDS             2142..3023
                     /locus_tag="IWO09_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263427.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWO09_000814"
     CDS             3026..3796
                     /locus_tag="IWO09_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0263428.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWO09_000815"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWO09_000815"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0263429.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWO09_000816"
     CDS             5107..6270
                     /locus_tag="IWO09_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0263430.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWO09_000817"
     CDS             6387..6797
                     /locus_tag="IWO09_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0263431.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWO09_000818"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWO09_000818"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0263432.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWO09_000819"
     CDS             9337..9654
                     /locus_tag="IWO09_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263433.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IWO09_000820"
     CDS             9814..10050
                     /locus_tag="IWO09_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0263434.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IWO09_000821"
     CDS             10047..10445
                     /locus_tag="IWO09_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0263435.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IWO09_000822"
     CDS             complement(11112..12056)
                     /locus_tag="IWO09_000822"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0263436.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IWO09_000823"
     CDS             complement(12352..13296)
                     /locus_tag="IWO09_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0263437.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="IWO09_000824"
     CDS             13517..14731
                     /locus_tag="IWO09_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0263438.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="IWO09_000825"
     CDS             14753..16768
                     /locus_tag="IWO09_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ0263439.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="IWO09_000826"
     CDS             complement(16851..18224)
                     /locus_tag="IWO09_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0263440.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="IWO09_000827"
     CDS             complement(18250..18534)
                     /locus_tag="IWO09_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0263441.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="IWO09_000828"
     CDS             complement(18536..20551)
                     /locus_tag="IWO09_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0263442.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="IWO09_000829"
     CDS             complement(20730..21035)
                     /locus_tag="IWO09_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0263443.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="IWO09_000830"
     CDS             complement(21124..22155)
                     /locus_tag="IWO09_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0263444.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="IWO09_000831"
     CDS             22362..23237
                     /locus_tag="IWO09_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0263445.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="IWO09_000832"
     CDS             complement(23389..24231)
                     /locus_tag="IWO09_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0263446.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="IWO09_000833"
     CDS             complement(24323..26200)
                     /locus_tag="IWO09_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0263447.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="IWO09_000834"
     CDS             complement(26197..27618)
                     /locus_tag="IWO09_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0263448.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="IWO09_000835"
     CDS             complement(27621..31010)
                     /locus_tag="IWO09_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0263449.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="IWO09_000836"
     CDS             31267..32658
                     /locus_tag="IWO09_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0263450.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="IWO09_000837"
     CDS             complement(32731..33378)
                     /locus_tag="IWO09_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0263451.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="IWO09_000838"
     CDS             complement(33386..34072)
                     /locus_tag="IWO09_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263452.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="IWO09_000839"
     CDS             complement(34078..35151)
                     /locus_tag="IWO09_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0263453.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="IWO09_000840"
     CDS             35373..36047
                     /locus_tag="IWO09_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0263454.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="IWO09_000841"
     CDS             36052..37392
                     /locus_tag="IWO09_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0263455.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="IWO09_000842"
     CDS             complement(37541..38527)
                     /locus_tag="IWO09_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0263456.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39251..40282
                     /locus_tag="IWO09_000843"
     CDS             39251..40282
                     /locus_tag="IWO09_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0263457.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40391..41923
                     /locus_tag="IWO09_000844"
     CDS             40391..41923
                     /locus_tag="IWO09_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0263458.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42062..42874
                     /locus_tag="IWO09_000845"
     CDS             42062..42874
                     /locus_tag="IWO09_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0263459.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42864..43349
                     /locus_tag="IWO09_000846"
     CDS             42864..43349
                     /locus_tag="IWO09_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0263460.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43372..43917
                     /locus_tag="IWO09_000847"
     CDS             43372..43917
                     /locus_tag="IWO09_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0263461.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43990..45633)
                     /locus_tag="IWO09_000848"
     CDS             complement(43990..45633)
                     /locus_tag="IWO09_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0263462.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45762..47990)
                     /locus_tag="IWO09_000849"
     CDS             complement(45762..47990)
                     /locus_tag="IWO09_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0263463.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48023..48502)
                     /locus_tag="IWO09_000850"
     CDS             complement(48023..48502)
                     /locus_tag="IWO09_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263464.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        14822 a         8973 c         9169 g        15738 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga aggaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat
    38881 acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga
    38941 gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg
    39001 ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag
    39061 gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc
    39121 ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac
    39181 aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag
    39241 ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac
    39301 acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt
    39361 aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt
    39421 attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa
    39481 tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac
    39541 ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca
    39601 ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac
    39661 aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga
    39721 ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga
    39781 agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa
    39841 gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa
    39901 tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt
    39961 tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg
    40021 ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac
    40081 agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact
    40141 atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca
    40201 ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt
    40261 cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt
    40321 attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa
    40381 tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca
    40441 agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt
    40501 cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc
    40561 ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc
    40621 cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat
    40681 gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc
    40741 tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa
    40801 atcaaaacag attcctattc tttatttaat ggaacaacct gctttgtctg tttctcaatc
    40861 atttttagac aaaaatgaaa tttcttatat gacccatact tctggtacaa ctggtatccc
    40921 taaacttatt tgtcattctg ctcattctat gggatggcgg acgaaatggc aaaaaacagt
    40981 tttcacaaaa atttcggaaa agaaattaat cgcttttcac atctctccag tgcattccag
    41041 attcaatatt ggtatctctt ctttgatgtc catgggattt cctatgatgc ctttagcaaa
    41101 cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac cggccaattg cactagagac
    41161 ccatccgaat aactttgttc agtggcgttt tactgctaag gagcatccag aagctttttc
    41221 gggtatccga tactatcact ccacttttga tgctataaac aaccagacaa tgaagacatt
    41281 tttgcgaacg tcgatggcac aggatgcggt atttcttcaa gtatatggcc aaagtgaatg
    41341 cggaccaatg attcttaagg ctcatacact agagagtttg aaaacaagtg atgcaagaga
    41401 tatgggcgtt ggactggaag acatgacaag cgctcggatt acagattcag ttgggaatgt
    41461 cctacctgaa aacacagatg gtcatataca attactgtct aaaggacggg ctttgactta
    41521 ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc gattggtggg atagcggaga
    41581 ttacggtttt atggataagc atggccacct atttttgaaa gatcggcaag tggatttgat
    41641 cgaaacgatc aatagtacat tagctattga agacttttta ttagattcgt tagatttttt
    41701 ggcagaagta gtgattgtaa gggataaaaa tcattctcca caaccgatta ttgctttagc
    41761 tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa gtccatgaac ttcctcgctt
    41821 gaacgtccct atcatccgtg attttgatgc aatcccgcga acagctacaa tgaaagtgca
    41881 acggcttcaa atcgagaaag aattaaaatc acaaaagtcc taactattta aggaaagaaa
    41941 gatccatttt ggtctttctt ttctttttgt ttttttaaat tggatgagat aaaaatatgc
    42001 caattggctg tttttctttt ttgaaaattt cgttatactc ttattcatgg aggaaaatgt
    42061 tatgaaaaga atcttgacaa ttggcggttc tgatccattt gctggtggag gaatccaaac
    42121 agatttaaaa acttttgaga attttggtct attcggacta agcgccttga cttctatagg
    42181 ggcactagat attaatgagc gatttatgct ggaatctata tctttggaat tgcttaaacg
    42241 acagttagtt tcgattgata aaatgacttc attagatggt atcaaaattg ggttattgaa
    42301 ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa acaaaacaaa atatcccaat
    42361 cattttagac cctgttttgg cattcaaaga aactgaagct gtgatcaatc aattgtatat
    42421 ttcgcagctc atcgaagaac tttttccact tgttacgatg attacgccta atttggcaga
    42481 agctgcttta ctttctggtc aagctcttcc ctcttcgtta acagaaatga tccatctcgc
    42541 taaagaactc actgcttcag gaacaaaaaa cattgtgatc aaaggcggca caaggatggc
    42601 tggagatgaa gctgtggatc ttctctattc aattgatcat tatgaggtat tttctcataa
    42661 aaaaatcgac actacaacag taaacggggc cggttgtgcc ttttcttctg caataacagc
    42721 gaatcttgtg ttaggggcag attttaccaa agcagtagca gaaagtaaaa aatttgttgc
    42781 ccattgtatt tctaatggcg tgctgatgaa cgatcatacc ggaagtgtct ggtctggcgg
    42841 tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt aacgatctat gctatgctga
    42901 ttgcgttgac agttgccctt tcattaacaa ttttgatccc tgtaccggct acgaatggat
    42961 ttgtgacatt atgcgaggcc gggatctata ccgctgcttt tctttatggt ccaacgggtg
    43021 gattagttgt tggtgcttcc agtggtctat tgatcgattt gatttcagga tatccgcagt
    43081 gggcagtttt ttcattagtg atccacgggt tacaaggata tgttgctggt tctttcagca
    43141 aatcttctac tcgtatgtgg ggattagggc ttttattagg aagcttagtt atgatcatcg
    43201 gctatggtat cgccggttgg attttatatg attggccggc tggagtggct tctataccag
    43261 gaaacatctt ccaaaatctg attggaatgg gacttgcctt tcctataact gcaagtcttc
    43321 aacgtattca agcacggaag caatattaat tgaaaaagaa ggttttagaa aatgaagttg
    43381 tcagaaaaag aattaaaaga ccaattgact gaaatagtta atgacatatt ggctgaggca
    43441 cacttgaaaa aaggtgatct ttttgtttta ggatgcagca caagcgaagt cgttggagga
    43501 catattggaa aaaattccag tgcggaagtt ggtcaatgga tcatccgtac gttaaaagag
    43561 ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt gtgaacattt gaaccgagca
    43621 ttagtagtag aacgaacagt ggcagaagca aaaaatttcg aaattgtctc tgttgttcct
    43681 gcactacatg ctggaggtgc ttgttctgtt gctgcttttg accagttcaa tgatcctgtc
    43741 gaagtggaac acgtagttgg acaagccggt atcgatatcg gtgacacatc aatcggtatg
    43801 catatcaagc atgtccaagt acctgtcaga cctcgtttga aaacccttgg acaagctcac
    43861 gtgacagccc ttcgctctcg tcctaaatat atcggtggtc ctagagcgaa ttattgaaat
    43921 gagtaattga cagagcaatc tgaaaaactt cctcgcaaag tccaactaat gaactttaac
    43981 gaggaagttt tacttgtttg ttttatataa ccgagcttca taaggtgcta gtatgccttt
    44041 agcttcaaat tctccgctag aggaatttga caagagtaat gtccaggact gatctgcttc
    44101 ttttggaagt tcatagtcag caagtgattc tgttaagttt accataataa gatattgttc
    44161 atcttctaaa tatctttcat agacataaag ttgtggatgt tcatgaagat attctttata
    44221 agaaccgtat atcagacctt tattggtttg tctaatgcgg atcatgttct tatagaattg
    44281 caggaccgac tctggttcat attcttgttg ttctacattg atcgttttgg tatttggatt
    44341 agtgatcagc caaggttcat gtgtagaaaa accagcatat tgttccgaag tccattgcat
    44401 gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata tccaatgctt cttctcttgt
    44461 cttaccttcc gcaagcagcc ttttataaag tcttttcgaa tctactgcat caacttgatc
    44521 gatcgattca aagggcatat tcgtcattcc gatttcctgt ccttgataaa taaatggcgt
    44581 accttgcaaa aagaaataca tcatagcgat tgcttttgca gacatagccc aaaaatcggg
    44641 acgagtatct ccaaacactg aaacggcacg aggaacatca tggttttcca tatataaagc
    44701 gttccatccg ataccatcca atgaagtttg ccaagcgctt aatgcatgtt tcaatttaag
    44761 gacatctaat tgcccttctt tttcttgctg ccagatatgg atatgatcaa attcgaatat
    44821 catatcgaaa taaccgtctt tccctaccca ttgtggtcct tcttctgcag tgacaccact
    44881 tgcttctccg acagtcatga tatcgtactc ttcaaaaaca tgtttcaaat cggtcaaatg
    44941 ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg ttctctgtgg cttttactga
    45001 atattcatct ttttttacat gagaaatcgc atctaaacga aatccatcta tccctttatc
    45061 caaccaccat cggatcatat tgaatagttc ttctttcaat ttctcgcttt cccaatttaa
    45121 gtctggttgt tcttttgcaa aaacatgcag ataatactgt ccagtttcat gagaatactc
    45181 ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt tctttgccat ctaccccgtc
    45241 gacccaaatg taataatcgc gatacgggct ttctttagat tttttcgatt cttcaaacca
    45301 ttgatgctga tccgaactat ggttgatgac aagatccata atcactttga tccctaattc
    45361 atgtgcttct tttagcaata aatcaaaatc ttccatcgtt ccgaatctct cttctatatc
    45421 ttgatagtca cttacgtcat aaccattatc gacaaatgga gattgataga tcggattgat
    45481 ccagataaag ccgataccaa gatctttcaa ataattaagt ttttcacgga tcccattgat
    45541 gtctccttga ccatcgccat tgctatcttt gaaactagca ggatagattt gatagcctac
    45601 tgcttctttc caccattttt gttcttgtat catatcgttt cactccttta acggtccctc
    45661 ttgattataa ccgattctat tttagaagta tccttgttac cggtaactag aaacgaaaag
    45721 ctcaacgaat ctgctaagtg cgttcgctga gctttatttt atcatagata gcgggcaatc
    45781 agttgaatca tttgcttggg aaattcattc aagtcagtaa tatctacaaa acgactttca
    45841 ccgtagatcg acttgatttc ttctttgtct tggccaatag ctgcggagac aaataggatt
    45901 ccttgacgtt catattcttt caaaacagac tggatatctt cttttgcttt tttgcctgta
    45961 taatccggca acgcttttgg ctgaccgtca ctaatattaa gcaacagttt ggttgttgcg
    46021 gattgtctgt ttaatttttc tgctgctaaa cgaagtgcag caccatcacg attgttttgc
    46081 cttggtttca tagtgactaa tttttcattt aaccattgaa atccgtcttc aaattcttta
    46141 taagaaaaca aagaagtctt ttctcttgac gaacggtctg ctgtatctcc gtaaatcaac
    46201 aatgggatat cgactttctt agaaaattcg gcaatggcaa tcgctgcttt ttttgccgct
    46261 tcgatccgat catcccgtat cattgaccca gattcatcga tccgtacagc taccgctaaa
    46321 gaaggttgct catgtggcgg gttctttttg tcaaaatttc tcaaatcacc atatgctact
    46381 ttgcttgcat tgaaccgctg accttcgtat tttccttttt ggtacgtctc tgtttgttca
    46441 ttatccagta actctaaaat ttgccggcta agtgattcta ctattggcat tacttgcatg
    46501 tgtagctcac gtgcttccgg cgtttttcct tggaaagctg gtcggtgaac gatgagacct
    46561 tctttttcat gaatcgtttc cttcatgatt tgtcgcgctt ctttattcag ttgcttcttg
    46621 atttccttat catgtgcttc catgtcagaa gcagacattt cttgagaatc atctcctgtt
    46681 tgttgttctt tttgattttt tccttcgata tcgctactgc gttctttttg catttgagga
    46741 ggaatctcgt ttttttctat tgtttcttct tgtttctctt ctccctcatt tggttcttca
    46801 gcagacaacg atttcttttc atctaagcgc agaatttctt cagcattttc agtgatgttt
    46861 cgttcatttg cttcttcggc gctaatggtt ctttcttttg tatgctcaga ttgaggatga
    46921 ccccattttt cttttctcaa tatgtcctga agttccagta gcaatccact gtcagacaaa
    46981 gcttgttcaa gaattgccaa ttcatctgaa tcagtggata atttatacaa aacttttgga
    47041 taaatcgact ggagtaaatc ttgtccttgc ttataggtat ttgcccaata aaaataagaa
    47101 cgcattcctg ctacaccttt gatggcgtta gcttttgcag tttcatccaa caaacggata
    47161 atctctgcca tctttaagag aagttcttgt ttttggatca tagttttcgc catcgcacgt
    47221 tctgccatga cttctaaggc aggaagatcc attttttccg catgttgcat ccgatctctt
    47281 aaagattcat ttaatgggcg atttccttgg taatttcgat ttgtagtgat cacaatgacg
    47341 caatctggat ggcgacgaat gattcctgtc ggtaaattca acatcccatt tggttcgagc
    47401 gctgagttaa gtgccacaag aacagaggca tctcggatga cagtcggctc ttgaatctct
    47461 aataaatagc ctttttcaat cgctcgaacg atttcagaag ggtaatagct gtaatggatt
    47521 gcatctggat aatcagcttc gatcctttga actagctctg ctaatttttc tttagcagaa
    47581 aaaggatcaa tgccgtaatg gttctggatc agtgtcagta cagcttcaag tgtatctgtt
    47641 tggtaaatag cttctagtaa ttcttggtct tgttccttat tagaatctac tacaggtaat
    47701 aaggctccga aaacatctga tttgtccata tctgcaaaac aggtcacttt tgtataggga
    47761 agttgcagat cagcagatag tgctttggct aattgtgtct tccccgaccc ggcatctcct
    47821 tccaaaagta cgttactgat tttcatttca ggatcttgcc aattcccttt gatttcggag
    47881 gcgatccgcc attcttctgc acttgtccga tggcttttcg gtttcttcca gatcatttgc
    47941 ttttctagcc gtgaaaaggt tcggttttct gttagtgaga aattctccat gcttctcttc
    48001 cccttttgat catctctgtc tattaatatc ctagctcatc taaaagacgt tttaaaatac
    48061 ggaatcgttc cccaatcagt tctccgtctt tttgtagtgt atcgtaatac aaaatgatgt
    48121 cttcatcgat ctttggattt ttggtatcta cttctaccgt cattcggttc ccttgtaatc
    48181 caatgatgtc tacgactgga ttttctggat cataaaattt ttcataacga taagcatctt
    48241 ggaagtataa acttccttgc gcagctaaga tcgctaaatc taaaatacgt gtgattggta
    48301 attcttccga ctgtcgtgac catttttctc cagtatgtcg ccataccttg cctgagatat
    48361 cgacttttcc acgatcattc cactgagcaa ggcccagtga aagccctttt gcatccatat
    48421 ttaatgcatt tcttccatca actttatcat agtcttctac aacgattact ggtttatgtt
    48481 ttaaatttgt tggtatttcc attattttaa ctcctcatcc ttttattttt agtaaataag
    48541 taaattagta gtttactcat ttactaaact atatgcgaca ttatagagga ttgaagtgag
    48601 gattgcaaag aaaaagagct tcattgatag aaataaaaac accatctcgt tcttttacac
    48661 gctcattagt aacatgtaga aagtccaaga tgatgttgga at
//
LOCUS       DACECJ010000015        46675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.015, whole genome shotgun sequence.
ACCESSION   DACECJ010000015 DACECJ010000000
VERSION     DACECJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.015"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(153..551)
                     /locus_tag="IWO09_000851"
     CDS             complement(153..551)
                     /locus_tag="IWO09_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0263465.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IWO09_000852"
     CDS             complement(548..1321)
                     /locus_tag="IWO09_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0263466.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IWO09_000853"
     CDS             complement(1318..2001)
                     /locus_tag="IWO09_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0263467.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IWO09_000854"
     CDS             complement(2168..3055)
                     /locus_tag="IWO09_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0263468.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IWO09_000855"
     CDS             complement(3052..3921)
                     /locus_tag="IWO09_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0263469.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="IWO09_000856"
     CDS             complement(4058..5344)
                     /locus_tag="IWO09_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0263470.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="IWO09_000857"
     CDS             5617..6195
                     /locus_tag="IWO09_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0263471.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="IWO09_000858"
     CDS             6195..7124
                     /locus_tag="IWO09_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0263472.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="IWO09_000859"
     CDS             7121..8857
                     /locus_tag="IWO09_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0263473.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IWO09_000860"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IWO09_000860"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0263474.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="IWO09_000861"
     CDS             complement(9753..10262)
                     /locus_tag="IWO09_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0263475.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IWO09_000862"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IWO09_000862"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0263476.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="IWO09_000863"
     CDS             complement(11563..12654)
                     /locus_tag="IWO09_000863"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0263477.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IWO09_000864"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IWO09_000864"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0263478.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IWO09_000865"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IWO09_000865"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0263479.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IWO09_000866"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IWO09_000866"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0263480.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="IWO09_000867"
     CDS             complement(16617..17591)
                     /locus_tag="IWO09_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0263481.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="IWO09_000868"
     CDS             complement(17572..19455)
                     /locus_tag="IWO09_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ0263482.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="IWO09_000869"
     CDS             complement(19473..21698)
                     /locus_tag="IWO09_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0263483.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="IWO09_000870"
     CDS             21813..22847
                     /locus_tag="IWO09_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0263484.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="IWO09_000871"
     CDS             complement(22914..23927)
                     /locus_tag="IWO09_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0263485.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="IWO09_000872"
     CDS             complement(24005..24193)
                     /locus_tag="IWO09_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0263486.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24633..24761)
                     /locus_tag="IWO09_000873"
     CDS             complement(24633..24761)
                     /locus_tag="IWO09_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0263487.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24764..26137)
                     /gene="rlmD"
                     /locus_tag="IWO09_000874"
     CDS             complement(24764..26137)
                     /gene="rlmD"
                     /locus_tag="IWO09_000874"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0263488.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26248..27303)
                     /locus_tag="IWO09_000875"
     CDS             complement(26248..27303)
                     /locus_tag="IWO09_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0263489.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27313..28743)
                     /gene="gatB"
                     /locus_tag="IWO09_000876"
     CDS             complement(27313..28743)
                     /gene="gatB"
                     /locus_tag="IWO09_000876"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0263490.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28740..30212)
                     /gene="gatA"
                     /locus_tag="IWO09_000877"
     CDS             complement(28740..30212)
                     /gene="gatA"
                     /locus_tag="IWO09_000877"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0263491.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30212..30517)
                     /gene="gatC"
                     /locus_tag="IWO09_000878"
     CDS             complement(30212..30517)
                     /gene="gatC"
                     /locus_tag="IWO09_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0263492.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30623..32659)
                     /gene="ligA"
                     /locus_tag="IWO09_000879"
     CDS             complement(30623..32659)
                     /gene="ligA"
                     /locus_tag="IWO09_000879"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0263493.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32676..34910)
                     /gene="pcrA"
                     /locus_tag="IWO09_000880"
     CDS             complement(32676..34910)
                     /gene="pcrA"
                     /locus_tag="IWO09_000880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0263494.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35065..36624
                     /locus_tag="IWO09_000881"
     CDS             35065..36624
                     /locus_tag="IWO09_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0263495.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36735..38666)
                     /locus_tag="IWO09_000882"
     CDS             complement(36735..38666)
                     /locus_tag="IWO09_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0263496.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38684..39598)
                     /gene="pfkB"
                     /locus_tag="IWO09_000883"
     CDS             complement(38684..39598)
                     /gene="pfkB"
                     /locus_tag="IWO09_000883"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0263497.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39595..40347)
                     /locus_tag="IWO09_000884"
     CDS             complement(39595..40347)
                     /locus_tag="IWO09_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0263498.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40506..40715)
                     /locus_tag="IWO09_000885"
     CDS             complement(40506..40715)
                     /locus_tag="IWO09_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263499.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40726..42054)
                     /locus_tag="IWO09_000886"
     CDS             complement(40726..42054)
                     /locus_tag="IWO09_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263500.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(42419..43006)
                     /locus_tag="IWO09_000887"
     CDS             complement(42419..43006)
                     /locus_tag="IWO09_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0263501.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43089..44339)
                     /gene="clpX"
                     /locus_tag="IWO09_000888"
     CDS             complement(43089..44339)
                     /gene="clpX"
                     /locus_tag="IWO09_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0263502.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44586..45875)
                     /locus_tag="IWO09_000889"
     CDS             complement(44586..45875)
                     /locus_tag="IWO09_000889"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0263503.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46029..>46675)
                     /locus_tag="IWO09_000890"
     CDS             complement(46029..>46675)
                     /locus_tag="IWO09_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263504.1"
                     /translation="RKLIASTDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSE
                     ELPSLEPARQLASIDEIEQLPYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHP
                     VFYLTIEEKLVKVIPKDLPKPQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAI
                     VYPVYDTYIEDPKRWFQLTVEACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
BASE COUNT        12896 a        10093 c         8336 g        15350 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattga ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taactaaaaa gactaagaaa caataacaaa
    24601 agttaacact tcttagtctt tctttagcac gtctatttat taaagatttt agggcctttt
    24661 ctcatgtttt tacgatcatt cttaggtgtt tccatttgtt tggatcggcc tccaccttgt
    24721 ttctttttga tcaattcttt cattttttct ttttggtcca tttttattct cctttaacga
    24781 atgaagcgac taattcgata tgatgggttt gcgggaaaag atctacaggc tgaactttct
    24841 tcacggcata acctgcttct gcaaataact tagcatctct tgcaaaagtt gccggattac
    24901 atgagatata gacgattcgt tctggtgaca tttcgattgc tgattcaata aaatgatgat
    24961 ctaatccttt tcgtggaggg tcaactacga tcacagatgg tttgatccct ttttcttgcc
    25021 attttttcat aactttttct gcttttccga cttcataatg aacattttcg attttattat
    25081 ttaaagcatt gaactgtgca tcttcaattg cttctggaat aacttccatc ccatacacct
    25141 tatccactct gtctgctaaa gacaagccaa tcgtacctat tcctgaataa gcgtcgatca
    25201 caatgtcttt tttatctaac tcggcaaact cgatggctgt ttggtacaga tactctgctt
    25261 gttgagtatt tacttgataa aatgactttg ctgagattcg ataggttttt cctaatagct
    25321 gatcttcgat atagtctctg ccatataaaa ctttttcttt gtctcctaaa atcacatttg
    25381 ttttttcttc attgatattt tggatgaccg aaaccacttc tggcaattct tcatgaatag
    25441 cttcagcaat ttccttggat ttaaagaatt tttctttacg agtaactaaa acgaccatca
    25501 tttcatggga ataatgacca cgacggataa tgatgtgacg aagctgtcct tcattttttt
    25561 cttcattata tcctcgtact tggaaccgtt gcaagatatc tctgactact ttgattgctt
    25621 gatcgatgac aggatcttgg atataaaaat cttcgatagg gactagatca tggctattct
    25681 ttttgtaaaa accggtggct aactgtccat cgatacggcg aacagggatc tgtgctttat
    25741 tacggtagcc gaatggttct gccattccga ccgttggaag tacaggaact tctggcatct
    25801 tagcaatctt cgccattaca tgctctactt gtttttgttt gaagacaagc tgttgttcat
    25861 acttcaaatg ggctaaaggt gcgataccta tgcgaagtaa tgcattgttt tctacaggct
    25921 gacgatcagg tgattgtgta ataaatgttt cgacttttcc atatccaaac ttattgcctg
    25981 cttttactac tagtatttct acttcttctc cagggagtgc attttcgata aataatggat
    26041 aaccatcgat ttttgctacc cccattcctt cataactcaa gtcgacgatt tcgactgtat
    26101 atcgttcgtt tttctttact atttgctgtt ctgtcatatt ttgctccttt aagcctattt
    26161 ttcaatccta ctgaactcta ctataaatca gctaattaca caactaaaac cggcactgac
    26221 cttactacgt tcagcaccgg cttttgatta attcttttga ttaggtgttt cgtctgctat
    26281 ttttccgtca ccgtcgatat cttcatccgt taaacgttcg acttctttta cgaattcttt
    26341 gctgacaacc tctaattctt cttcatctgt acctgtgatt gcttcatcag gaatctgatc
    26401 ggtattggca aagaactcaa tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc
    26461 gccgtattca ccatctaagt tgatcattac tggtcgattg atatcatctt ctgtttcaac
    26521 gaacaaactg cttgttttgg tatagattat tcttggatca tcgacatgtc gaccgccatt
    26581 cagcatgagt gctgcaatat gcaagatctc aaagatattt gctgtcttca caatgattaa
    26641 agaaaacttc ccatcatcca atttggcatc tggcgcgatc tgttcgaatc ctccaacaga
    26701 gttcgtcagc cctaggaaaa acatcgaagc atttccgata tattcccctt catcatactt
    26761 caagcgcatt ttgattggct ttactcgagg gagcatctct gctcctttcg caagataagc
    26821 aagataacca aaaatactct ttagttctga tggaacttca taagtcaatt ctgtcaaata
    26881 gccacctgca gcgatattga tgaagtaact gttatcagaa aggccgatgt ccatttttac
    26941 ggtttggtgt ttttttatca cttctgctgc tgctttaata ttatctctag gaatttttaa
    27001 tgctcgagca tagtcgttcg ttgtaccagc tgggatgatg gccattttag gacggtgttc
    27061 aagcggagcg attccattaa ccacttcgtt gattgtgcca tcgccaccag ctgccactac
    27121 tagatcaaaa cctagttctg cgactctaca tgcttcattc ttggctgaat ctggttctgg
    27181 tgtagttgca taggcgctgg cttcaaatcc tgcttcttct aatgcctgta ggatatccgc
    27241 caaatttttt ttcaataatt cttttcccga ggtggggtta taaattacgc gtgctttttt
    27301 cattttcagc ctctatcgtt tcgctaattc atctttaagt agcttattca cgacaccagg
    27361 atttgcttgt cctttagttg ctttcatgat ttgaccgact aggaagccaa ccgcacgatc
    27421 ttttccattt ttgaaatcat cgacagattg ttggttgtta tccaaaattt cattaatgat
    27481 tggcagtagt ttgttcggat cagataattg cacccaacca tttttttcaa ctacttcttg
    27541 ggcttctccg ccatttgtga tcaattcacg gaagactttt ttggcgattt ttgagctgat
    27601 cgttccatcg gcgatcaaac gaatcattcc tgctaagttc tcaggtgtca attttgtctc
    27661 agctaattcc agtttctcac tgttcaagta agcagaaact tcacccatca accagttaga
    27721 tgcttgcttg gcatctgcac cattttccaa aacagcttca aaaaaatcag acatttcttt
    27781 tgtaagtgtc aataccattg cgtcatactc tggaagtcca aactcacgga tatagcgttc
    27841 gcgacgagaa gctggcatct caggcaaact agcttttact ttttgaatcc attcgtcatc
    27901 gatcacgaat ttcgggatat caggctctgg gaagtaacga taatcactag acccttcttt
    27961 gacacgcatc aaaactgttt tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat
    28021 ttctcctcct gaaagaagaa cttttgcttg acgtttttct tcaaaagcta aacctttttt
    28081 cacgaaagat aaagagttca agtttttcaa ttctgctttc gtaccgaact cttcttgtcc
    28141 ataagggcga agagaaatat tggcatcaca gcgcatagag ccttcttcca ttttcacatc
    28201 gctgacttct gtaaattgga taattgatcg caatgcttct agataagcgt atgcttcttc
    28261 tggtgaacgc atatcagctt cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa
    28321 atcaacgtaa gaataaccgc caataccatg catgtttttt ccagcatctt cttctaaatg
    28381 gacacgttcg atccggattt ttttcttttt accttctact tcgatttcga tccatccgtc
    28441 atgaccgatt ggttgatcaa attgggaaat ttgatatgct tttggattat ctgggtaaaa
    28501 gtaatttttc cggtcaaaat gagtatcttt agagatttca cagtttaatg ccaaagctgc
    28561 tttcatcccg aattccagcg cagctttatt cattactggc agcacgcctg gataacccca
    28621 gtcgatcacg tttgtattgc tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa
    28681 aatttttgag ttggttttta actcgacgtg gacttctagt ccaatgactg tttcaaaatt
    28741 cattcgtctt tccctcctaa aatagctggt tgttgtttat ggaattctgt tgcttgttca
    28801 aacgcatatg ccgcttgata catcgttttt tcatcaaaat aattaccgat gatctgcaat
    28861 ccaactggta atccttctga taaacctgcc ggtacagaca tacctggaag tccagccaag
    28921 ttgacaggga tcgttaagat gtcactcatg tacattgtga ttggatcgtt aatgttctca
    28981 ccgattccga atgcaactgt tggtgaagat ggcccgatga tcaagtcata atctgcaaag
    29041 actttttcaa aatctcgttt gatcaatgta cggacttgtc ctgctttttt gaagtaagcg
    29101 tcataataac ccgcgcttaa ggagaatgta cctaacatga tacggcgttt tacttcttca
    29161 ccgaatcctt ctgaacgtga gttgacatat acatcatcta atgatttcac attttcagag
    29221 cgataaccgt agcggatacc atcgaatcgt tgcaaattgg aacttgcttc tgatgaggca
    29281 atgatataat aaacagccac accgtatttt gaatgaggca agctgacttc ttccacaaca
    29341 gcacctaatg aacggaatgt ttctgctgct ttcaatacag cttcttttac gccaggggcc
    29401 acaccttcgc ctaagtattc tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc
    29461 agtccctctg caaaatcagg aactgaaacg cctgaagaag taccgtcttt ttcgtcataa
    29521 ccgctgatag ctgtcaatgc taatgcatta tctttcaccg tacgggtcat tggtccgatt
    29581 tggtcaagag atgaagcaaa ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt
    29641 ttcatcccta cgatgccgtt aaaagctgct ggttggcgga tacttccccc agtatcacta
    29701 cctaaagaaa ctggaatttg tccagctgca actgctgctg cagaaccacc agaagagcca
    29761 ccaggtactt ttgttgggtc ccaagcattt tttgttttct tgaaataaga agtttctgta
    29821 cttccaccca tagcaaattc atccatgttt agcttaccaa cagggatcat atctgcttga
    29881 tagattttat ccatgaccgt tgcatcataa attggttcaa aattgtaaag gatcttagat
    29941 gcagcggttg tcaagatatc ttttgttacg atattatcct tgataccaat tggaatacca
    30001 gcaaggacgt tgctttcatc aatccctttt tcatctaaag cttttgcctg ttccaatgct
    30061 ttttcttcgt tcaatgtaat gaatgcctcg acctcaggct ctgtttcttt gattcgttca
    30121 aatgttgttt ttgtcaaatc ttgtacagtg atttctttcg atacaagcat atgatgcagt
    30181 tcttctagag aacgattgtg taattctgtc attatgcgcc agcctcccca ttatcgatga
    30241 ttgcgggtac tttgataaag ccgtcttctg tttccggcac atttttcaat aattcatcgc
    30301 ggttccatcc agcttgcgcc cgatcttgtc gcatcacgtt tactgtttca accacattcg
    30361 acgtaaatgg aacaccttcc gtatctactt cttccagcat ttcaaccata tcgatgattt
    30421 tacctaattg gtcggtaaaa cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt
    30481 tggcaacgtg tttcacttgt tcttcactaa tcgccatact accccttctt tctaaacgag
    30541 ttaaaaactt tgctctctat tttaacacaa gagtgccgtg tctgaaattg tttattgaaa
    30601 tttgtctgaa aaaactcatt tttcattctt ctactgagcg acttttagcc aatgcatctg
    30661 ccatttgatc ttctgtccaa acttcgattc ctaattcttg tgctttggtc agcttgctgc
    30721 cagcatcttc tcctgctacc acgatatccg tttttttcga tacgctacca gtcactttcc
    30781 cgccaagatt ttcgatcgtc tcttttgctt cttcacgagt aaatcgtgtt agtttaccag
    30841 taaggactac tgttttttct ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa
    30901 gccctttgta ttcgaaattg acgcctgctt gttttaattc attcatcaat tcatggactt
    30961 cttcgttact gaaataagtg actacactgt cggcaatgat atcaccgatc gtatcaagcg
    31021 caataatcga ctcatagtca cttttactca atgtttctag atcaccgaaa tgttctgcta
    31081 aaatttttgc tgctttcgct ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga
    31141 ccgaattatc tttgctgttg tcgattgctg tcaaaatatt atttgctgat ttttctttta
    31201 ttttgtccaa cgtcaataac tcttcttctg tcaatttgta aagatcagca acatctgcta
    31261 ctagtttttt atcatacatc tgttctagta cgcgcggccc caacccatcg atattcatcg
    31321 catttcttga aacaaagtgg ttcaaacctt ctttcatttg agcagggcat ttaggattga
    31381 tgcaccgtaa ggctacttct tcatccaaat ggacaagttc acttccacaa actggacaat
    31441 gtgtaggcaa ttggtattct tcgctgtctt taggtcgttt atctaacacg acttgcgaaa
    31501 cttctgggat gatgtctccc gctttataaa tcagaactgt atctttcaag cggatatcct
    31561 tcaacttaat ataatcaccg ttatgcaggc tggcacggct tactgtcgtt cctgctactc
    31621 ggactggtgt catgatagct gttggcgtta ctacccctgt tcgaccgact gtccattcga
    31681 tgttttcgat tagcgtttca acttcttctg gcggaaactt gtaagctgct gcccatctcg
    31741 gtgctttgat agtaaaacca agttgatctt gtagcgagaa ttcattcact ttgatgacga
    31801 ttccatcgat ttcatagggc aaatccacac gtttgtcatg atattcttca atgtatgacc
    31861 acacttcatc aatcgaatga catagacgtt tttcccgatt cgtgtggaat ccaatttttt
    31921 ctagctcttc tagcgcatca tactgggttt ttgcttgcat aggaccgaaa tcagctactg
    31981 tgtataaaaa agtgtccaga tttcttttcg ctgtgatttt agaatctaac tgacgcaaag
    32041 aaccagctgc ggcgttccga ggattcgcaa agatatcttt tccctctgct tctctgtctt
    32101 ggttcaattg gacaaatgac cgttttggca tgaaacattc gcctcttact tcgatgttca
    32161 ttggttcttt taatttgatt gggaccgacc ggacagtttt taggttttca gtgatgtttt
    32221 ctcctacagt tccatccccg cgagtcgcac ctctcacgaa atttccatct tcataacgca
    32281 aagaaacgga aagtccatct atttttagtt cacagcaata gtcaactaca cgtcccactg
    32341 ctttttgtac acgttgatca aaagctatca gctcttcttt gctgaaaacg tcgttcaaac
    32401 tgtaaagcgg aatatcatga gtgacttttt caaaaccttc taatactttt ccgcctactc
    32461 gttgagtggg agaatcagac gtgatcaaat caggatattg ttcctcgatt gctactaatt
    32521 ctgcgtattc tttatcataa acatagtctt ccactgttgg tttatcttct acgtagtatt
    32581 cgcgagacca ttggttcagt cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa
    32641 tcgtcatttc atgttccatc atttaccctc cactactaag ctttttcaat tggcgcaaag
    32701 gcagccaaaa gacgttttac tccttgactt gggaaagcca catctagttc tagatccttc
    32761 gacgtcccgc ttactcggac gactgttcct agaccccatt ttttgtgttt gactttatct
    32821 ccaggtttcc agctgttatt ttcccctcca gtagcacttt tagagggaac aaccgctgtc
    32881 tctggctgtt tatagctata tgctgttttt ttagcgaata caggcgtttt tttaggcgta
    32941 ggacgcatgc cttcgatttc cagtaactcc tgatcgattt cttctacaaa acgagaaggt
    33001 cggttatatt gcgtccgtcc atataatgtt ctggagaaag catttgttaa atacaacgct
    33061 tcttctgctc gcgtgatccc cacatatgcg agacggcgtt cttcctccag ttcgctttct
    33121 tccatcaatg ctcgagataa aggaaaaacc ccctcttcta gaccaatcag gaatacaact
    33181 ggaaattcta atcctttggc tgcatgcaaa gtcatcaatg tcacttggga agcatcttct
    33241 tctaaattgt caatgtcgga aacaagtgcc agatcattta agaagaccgt tagtttttct
    33301 tctggtgcat cagcgtcttc ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg
    33361 accgttaaaa attcctccaa gttttcgatg cgtgcttgtg cttctaatgt attttggatt
    33421 tttaagtctt ctagatatcc gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc
    33481 aagtatggga tcatctgagt gacttcttgg atcatctctc caaatgcacc taattgctgt
    33541 cctgcttttc caccgatatt tgccagatcc acattttgcg ctgcttcaag cagtggccat
    33601 tcatgaagag aagcaaaact gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg
    33661 ggcgaattaa cgatacgctc aaaactgatt gaatcttgag gatttaccag cacattcaaa
    33721 tacgctaaaa tatctttgat ttctttacga tcgtagaact tgtgccctcc aaccatttta
    33781 taaggaatat ttgctttcaa cagagtttct tccattacac gagattgtgc gttcgtccgg
    33841 tacaagatgg caaagtcgcc gtaattccga tgattgcttc gaatctcttc ttgcatccgg
    33901 tctacgataa aacgggtttc gtctcgttca ttatctgcac ggtagtaagt gatcttgttt
    33961 ccttctttgt tttctgtcca caagtttttt ggttttcgat tgctattgtt ttcaatgact
    34021 tggtttgctg cgctaaggat gtttttcgtg gagcgatagt tttgttctag caaaattact
    34081 gcagcatctg gataatcttt ttcaaaatcc aagatgttct gcatatctgc accgcgccag
    34141 ccgtaaatac tttgatctgc atctcccacg acgcacaggt tcctaaatct acctgccaat
    34201 aaattgacta gcgtatactg tgcgtgattt gtatcttggt actcatctac atgaatataa
    34261 tggaatttgt tttgataata cgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta
    34321 ttcatgatca ggtcgtcaaa gtccatacat tgattatatt gaagctcttt ttggtaagca
    34381 gcataacatt ttgccgcaat ctcttcgtaa taactgcctt gcatttttgc aaaatcctga
    34441 ggggtctgca ggctgttttt tgcttgagag attgttccta atatacttcg aggatcatat
    34501 tttttcggat caatgttcag ttcttttagt atccgtttca tcaaggtcag ctgttcagaa
    34561 gaatcgatga ttgtaaagtt ccggttatac ccgataaaat caacatctct tcttaaaata
    34621 cgcacacaca tcgagtgaaa agtcgatacc cagacatctt cgcctccaga tgcgagaatc
    34681 gcattgacac gttctttcat ttcttttgca gccttattgg taaaagtaat cgctaggatg
    34741 ttccaaggat tgacttcttt ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc
    34801 gtctttccac ttcctgcacc tgccattagc agtaatggtc cgtcagtgtg aagaacagct
    34861 tctttctgac gggggttcat cccgtttaat aattcagctt tggatgacat taacaacatc
    34921 ctttctttta tcaatcttgt taattatagc attttcttgg ctttcattaa actagctagt
    34981 tcaaaatcat ttgtttcttt ttatggattt ttccttgtca attcgttgat ttcgcgctaa
    35041 attaaaagaa agcgaggcgt ttgaatggaa aaaaaaggaa aaatcaagaa attagataag
    35101 actcagatat attttattct aaaaggaata tttgttggac tgctgacagg aatcgtcgtt
    35161 agcctgtttc gattatctat cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg
    35221 agtcgggaac agccgattta cttaattggc ctagctattt gctgcatcct tgcagcattt
    35281 ttcgtgggtt atctggtcaa aaatgaaccg gatatcaagg gaagcgggat tccacaagta
    35341 gagggacagc ttcgcgggga attatcaatg aactggttct ctgttttatg gaaaaagttc
    35401 attggcggtg tactttcagt tggtgcgggc ctttttctag gtagagaagg accttctata
    35461 cagcttggcg catccattgg acaaggagct ggacaattgt ttcggagtcc atcttcagaa
    35521 gaaaagattt taatttctag cggtgcaagt agcggacttg cagcagcatt caatgctcct
    35581 atcgcaggat tactctttgt tttagaagaa atccatcact ccttttctcc acttgtttgg
    35641 ctgacatcat ttgcttcagc aatcactgct aacttcgtct ctctttactt tttcggcttg
    35701 agacctgttt tatatctagg tgaccttcct tctttgccgt tgaaatatta tggttcctta
    35761 gtgatcttag gagtacttct aggcatctta ggttttattt atcagaatgt cttgctggct
    35821 ttgccaaaat tttacaaccg attgccttta ccagcttatt ttcacgggtt cgttcctttc
    35881 ttgctgattt tgccaattgg ttatttatgg cctagtactc taggcggagg caatcaaatc
    35941 gttttagctt ttggacatgc ttccttgcca atcattatga ttatcggttt gtttgttctt
    36001 cgattcgttt tttccatgat ttcttacggt gggggtcttc caggggggat tttccttccg
    36061 atattgacat tgggggcttt aatcggtagc ttttatggaa acattatggt tgatttcggg
    36121 atggatccaa tctacatcaa ggattttgtc gttattgcca tggctggtta tttcacagcg
    36181 attggaaaag caccgctgac agcaattatt ttagtgacag aaatggttgg ctctcttaat
    36241 catctgatgc ctttaggatt ggctgctctt gttgcttata tcgtcaatga ccttctcggc
    36301 ggtaatccaa tttacgaatc attattggaa cgtctacttg gaaatactca gaccgcctca
    36361 ctaaaaggtg caaggacaac ttttgatttt ccagtaacgg cagagagttc tttagacgga
    36421 ttaatggtac gagacttcaa ttggcctaaa aatatgctgc taatctctat acgcagagga
    36481 aatcaggaaa tcttgactca cggtgatacg atcatgaatg taggagatat tttggttatc
    36541 ttgactgatg aagcaaactt gccaagaatc aagcaggaaa tcgaggctaa atcttctatc
    36601 acaaatttga agcaatcatt ttaacgaaca tggaaaaact attttacaac aactattcat
    36661 ctacactact tgattctata tgattctata aaaaaagaag aggctgggaa aacccagcct
    36721 cttctttttt atttctatgc ttctgctact gcaggagctt ttctcaataa accataaatc
    36781 acaccagata cgactgctcc gatagcaata aacaccaaat acatgaccgg gtgactcagt
    36841 aagaatacaa cgaaaatccc gccgtgagga gccagtaatt tgattccgaa agctccgact
    36901 aatccgccag ttaaagcaga accaataata aaacttggaa tggcacgaat cgggtcagct
    36961 gctgcaaatg gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat gttcgtcaaa
    37021 ccagcatctt gatctgtttt gctgaattta ttcttaaaca aacgtgtagc aacgaaaatt
    37081 gccaatggag gtaccattcc tccagccata acagaagcca tcacgacact tccgccagtt
    37141 gcaacagtcg tagctaaagt tgctgtacca aacacataag cggctttgtt gattggaccg
    37201 cctaaatctg ccgccatcat tccagctaat aatgcaccaa gtaaagcagc attcgaacca
    37261 ctcatacttg tcaagaaacc gttcaatgca tcattgattg ctttcatagg gacattgacc
    37321 agcaacatca agaaaccagt aatcagtaag ccaaacactg gataaaagag gatcgtttta
    37381 attccatcca atgattttgg taagttcttg aataatttac gtaaaaatac gatgacataa
    37441 ccagctaaga atccaccaat cagtgcacct aagaatcctg caccgcctgt attagctaat
    37501 gcaccagcag caaatccaac gatcaaacca gggcggtcac cgatacttga agcaataaat
    37561 cctgctaata ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt gaaccagcta
    37621 gcagcttgat tgtaattccc tagatttgat aattgatcat gtggcacgcc aataaattgg
    37681 tcgatcatga aagaaagcgc aatagcgatt cctccaccaa taacgaaagg taacatgtgt
    37741 gatacgccgt tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc cgcatttcct
    37801 tcttctgaac tgctttcttt tccatcacca tggaatactg gtgcagtacc actaagggct
    37861 agattgatca actcttctgg tttacggata ccatcactga caggacgatt gatcagttct
    37921 tttccgtcaa agcgattcat ctctactttt ttgtctgctg caacgattac accttctgca
    37981 cgggcgatgt cttcagcagt caatcggttt ttgattcctt ctgaaccgtt cgtttcgact
    38041 ttgatctcca cacccatttc tttcgctttt ttcttcaaag catcttctgc catgtaagtg
    38101 tgagcgatcc ctgttggaca agctgtaacg gcaacaacga attttctttc tggatgagca
    38161 gaagcttctg cagcttgtgt ttcttgttct gctttttctt ctgcttcttt ttgctcttca
    38221 gctgtttgga acaagtcttg gacttcttct ggtgtatttg cttcttttaa ttttcctacg
    38281 aaatctgcat caatcagcaa tcgagatagt gctgctaatg cttgcaaatg tgtgtcgttt
    38341 gcgccttctg gagctgcgat catgaaaaac agataagttg gctgtccgtc taaggcttca
    38401 taatctactc ctttttggct ttttgcgaat aaaactgttg cttctttaac tgcactgttt
    38461 ttcgcgtgag gcatggcgat tccatcacca agacctgtag aagtttgtgc ttcgcgggct
    38521 agaattcctt ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa cattttttgg
    38581 accatttcat cgattgcgcc ttttttatca gtcgcttgca aatccatgat catcgcttct
    38641 ttgaccatta aatccttgat attcatgatt atcctccttg aaactaatca atttttgtga
    38701 tttcaacttc tggcagcaac tcatcaatca attcgcgagc tgccaagtca tcagaaaaag
    38761 ctgttgcgct tccgcaagca actccccatt taaaggcttc gattggatct ttcgtacggc
    38821 tgtaagcccc aataaagcca gcaatcattg aatcccctgc acctactgaa tttttcaatg
    38881 ggcgaactaa tacattcgtt ttataagttc cttgttccgt gaagagcaaa gcaccgtctc
    38941 cagccattga gatcagcaca ttctgtgccc cttcagctag taattttttc ccatacggaa
    39001 tgatttcttc tacagattgg aaggttgtgt tgtaaagctc agctaattcg tgattgtttg
    39061 gtttgatcaa caacggtttc ttaacaagtg cattttttag gtcatcacct gttgtatcaa
    39121 tcacgaaatc tgcacctttt tctcgaatga tatcaatcag ttcttgataa aatccttttc
    39181 gcaggctagc tggcgcgctt cctgacaata cgacgatatc tccttgtcct actgttccta
    39241 atgcttgctt caactcatcg atttcttcat ctgaaataga tgggcctaag ccattgatct
    39301 cagtttcagt gtctgatttt agtttgatat tgattcttgt atcttctttt acggttgtga
    39361 acaaagactg gatttgttct ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc
    39421 ctaaaaaccc tagtgctgta gattgagaat cgatccgttt caaaatacgg gaaacattga
    39481 tcccttttcc tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg cctagtttta
    39541 acccatctac gcgtacgata taatcaattg atggattaag tgttactgtg taaatcattg
    39601 tattgcctcc ttaattgttg tcttttcacg gaattcatta acgaattcta atggacaacg
    39661 ctcggttatg atcgtggctg cttccaaagg tgcaacagag gtgaaggaca actggttgaa
    39721 tttggtatga tcaatcaata caaatgtctc ttcggaattt ttcatggcta aacgtttaac
    39781 agctgcctct tctggatccg gagtcgtgaa tccttgttca agatgggcac cgttcattcc
    39841 cataaatgct ttattaaaat gaaaattttt tagttgttct atcgcattag atcctaaaac
    39901 agcattagtt gccagcttta tcgttccacc taaaataatg gtagcaagct ccaaatcaac
    39961 taaatttgcc gcatgttgaa cggaatttgt cactactgtg atctgttttc cttgcagaaa
    40021 aggaatcatt tctaacgtag tagagccagc gtctaaataa atgacatctt cgtcattaat
    40081 ggtttcagct gccgatttgg cgatttgctg tttttcgtga acgtttttga gtgatttttc
    40141 actcatattc tgctcaaaat tcaataattg ctctttctta gctccgccat gaactctttc
    40201 tagaaaccca ctattctcca gctcttgtaa atcacgtctt atcgttgatt cagaagcatt
    40261 caaaaggttt acaagatctt gtgatttcac gattttctgc tgttcaagca agtctaggat
    40321 tttttgtctg cgttcctctg taagcatttt cacgatcacc tcaatacaaa taatagcata
    40381 ttttttttct actagcaata taaatcagtc aaaaacgttc ataaaaatta taaatcagtc
    40441 aaaaaaaaat aaaaaaccag tcagaaagac tggtttttta tttttttgtc tatcatcaag
    40501 ccgttttatc gttttttatt ttcgcttctt gtttttttca tataaacaga agcaggtttt
    40561 ctttccttat gaagaagata ataataaagt cccatcccgc caaacatgac caaaaaaccg
    40621 atgactgatc gaataggaaa attaggaaca aaaaagagac agacggataa taatgatgcc
    40681 ggaaccatta ttcgataata gagttgttta tccattttct tcacctcata ttttttgtaa
    40741 agtaatcaat tgttctaatt ctcggtaatc taaaaattgc acacgcagat gctgctcacc
    40801 tttcttgaca tcgaattgga tacttcgccg ctttttcttt attcgtttga aggtaacatc
    40861 agagagtcta tgtttgattt cctttaacgg taattctgac agggattctg aaggaacata
    40921 gattcttaag gataaaagat actcttgata tttttcatct gttacctcta cttctagata
    40981 ttccctaata taataacaat aagcttcgat tccattatcc aaagcaacag tcggatcgat
    41041 aactttatag cacatcatgc gtgatcgatt ctcgtcaacc caaggaaaat acgcctgagc
    41101 atttagtgtt ccgcgaacac gcctttcttt ataatctcct tgttggatat cataactttt
    41161 tgttacaaaa aatgaagcat tctttttagg aacaggttgc atttgtgcat tctgatactg
    41221 gacagattgg atcgtatggt cagtgtaaat gtattcaacg aatggcggtt tcaataaacc
    41281 gacaccattg tcccaagcaa gatgatcaaa cccaagctgg tccctaaaat aggattgaaa
    41341 catttgattg tttggctttt gccagttaaa ggggtctcga taaagctgga tacatatgac
    41401 ttcgttttct aattccttca ctttttctac ttgttggatg tcgagaaaaa tcggtttagg
    41461 gatgatttga tgataatagc gactataata catttcccaa cacgaagtga aacataaacc
    41521 ttggtaaaaa agatcgtatc ctggaaattg gttgcaatcg actactactt ccccgttttg
    41581 ttgtttcatt ttaggtaaat gttcgatttc atcctttttc tcaaacaata gccgttgaga
    41641 gatctgtttt gtattgtgat agataaactc gctataagct cgcatatagg cgaacacccc
    41701 atgagcaatc atttttttct gaatgaagtc tcgaatcaaa aattgctttt ctttgaaaac
    41761 agagatagac aatacatttt ttatcaataa ttgattatct tccaagcgaa aactacattc
    41821 gttctcgtca tttcctacaa ctaaccaact ttcgtctctt accgatgcta gcattatttt
    41881 ttcttgaagc tttcgaagat catacggatc cgcttgttgt gtgaaagagc ggataaatgt
    41941 tggctggatc aacagacgtg tcaaaatagc ccaatcgctt atttcctgtt ttttttcttc
    42001 ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa tcattttctc
    42061 acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa actaaaactt
    42121 tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag aaacaaagag
    42181 gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact tttcgaatca
    42241 tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg atttctaaaa
    42301 ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa gctcaaaaaa
    42361 agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt tattcttttt
    42421 atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt tttgtaacgg
    42481 aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg atttcgctct
    42541 catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca acaattacag
    42601 ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa ggcttatgac
    42661 gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa tatgtctcga
    42721 tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca tatcccggaa
    42781 catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg gtctttcctg
    42841 gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata aaagaagatt
    42901 tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct ggatattgtt
    42961 ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc tctctcctta
    43021 acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt tacggcagcc
    43081 tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg ctcaccagac
    43141 aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga agggatatca
    43201 aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc accggtattg
    43261 cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc aagttcggta
    43321 ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg ctcagtcaag
    43381 atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg taatcgtccg
    43441 ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg ctgcatgaca
    43501 ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt ttcgcccata
    43561 cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa caatatgttc
    43621 gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg aggtggcaca
    43681 cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc gccggaaacg
    43741 tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc atcaatatag
    43801 atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa tttaagcaaa
    43861 atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc atctgcgatt
    43921 gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt accagaccct
    43981 gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt gttttcttgt
    44041 gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga gcgttttgcg
    44101 cgatcttgtc caatcacgta gttattaaat acttcaagaa tttctagtgg tttagggact
    44161 tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc tttacatagg
    44221 tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt cacttcttct
    44281 tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt gtcatacatg
    44341 gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt tacagtcttt
    44401 tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc aactgaccta
    44461 tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat cgtgtcacag
    44521 tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga tttattactt
    44581 gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc ttttttcatt
    44641 gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc gattggcatg
    44701 ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt tgcttgaaga
    44761 ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat ttctgcttca
    44821 ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata gtacatttca
    44881 ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg atgtacttca
    44941 tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc catacggatc
    45001 gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa ttctttgcga
    45061 tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt tgcgaattcg
    45121 tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt gaagactgct
    45181 tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt tacatctaga
    45241 ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa tgagcctgaa
    45301 cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc atctacaaag
    45361 ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc tttgataact
    45421 aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc atctgttact
    45481 tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac ttctggtttc
    45541 actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc aacatcgatt
    45601 tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata agcttctggt
    45661 aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg gttgaatact
    45721 tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt cactttatta
    45781 aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt taaaacacca
    45841 tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag tttagaaatt
    45901 cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa ccctttcaaa
    45961 ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat ctttttcact
    46021 gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt ttttaaaaaa
    46081 tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc ctctacagtc
    46141 agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata aacaattgca
    46201 gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc attcccgtaa
    46261 cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt tggcagatct
    46321 tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg atggcattcg
    46381 ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac ctctggcgat
    46441 acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc gatctcatca
    46501 atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt attgtggagt
    46561 gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg gattttttca
    46621 atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt tctag
//
LOCUS       DACECJ010000016        41473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.016, whole genome shotgun sequence.
ACCESSION   DACECJ010000016 DACECJ010000000
VERSION     DACECJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.016"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..386
                     /locus_tag="IWO09_000891"
     CDS             <1..386
                     /locus_tag="IWO09_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263505.1"
                     /translation="HQGNSKELEVVLRYNLFSFTSEFYFLRHKERIVGMVSIIVNPFQ
                     NLSYSIVNYLICEPAYTTTILTTVLENINEVSILEVKKLVIHLLNSTEDESLIPYLKD
                     SHFFKEAVLSKEYREHDVLRYAYIY"
     gene            450..785
                     /locus_tag="IWO09_000892"
     CDS             450..785
                     /locus_tag="IWO09_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0263506.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            1209..1472
                     /locus_tag="IWO09_000893"
     CDS             1209..1472
                     /locus_tag="IWO09_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263507.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            1788..2218
                     /locus_tag="IWO09_000894"
                     /pseudo
     CDS             1788..2218
                     /locus_tag="IWO09_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            2357..2779
                     /locus_tag="IWO09_000895"
     CDS             2357..2779
                     /locus_tag="IWO09_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263508.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            2924..3169
                     /locus_tag="IWO09_000896"
                     /pseudo
     CDS             2924..3169
                     /locus_tag="IWO09_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3278..4234
                     /locus_tag="IWO09_000897"
     CDS             3278..4234
                     /locus_tag="IWO09_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263509.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(5070..5741)
                     /locus_tag="IWO09_000898"
     CDS             complement(5070..5741)
                     /locus_tag="IWO09_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0263510.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            6058..7689
                     /locus_tag="IWO09_000899"
     CDS             6058..7689
                     /locus_tag="IWO09_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0263511.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7692..8771
                     /gene="uxuA"
                     /locus_tag="IWO09_000900"
     CDS             7692..8771
                     /gene="uxuA"
                     /locus_tag="IWO09_000900"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0263512.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            8845..10272
                     /gene="uxaC"
                     /locus_tag="IWO09_000901"
     CDS             8845..10272
                     /gene="uxaC"
                     /locus_tag="IWO09_000901"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0263513.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            10356..11003
                     /locus_tag="IWO09_000902"
     CDS             10356..11003
                     /locus_tag="IWO09_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0263514.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            11132..12508
                     /locus_tag="IWO09_000903"
     CDS             11132..12508
                     /locus_tag="IWO09_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0263515.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            12519..13313
                     /locus_tag="IWO09_000904"
     CDS             12519..13313
                     /locus_tag="IWO09_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0263516.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            13326..15092
                     /locus_tag="IWO09_000905"
     CDS             13326..15092
                     /locus_tag="IWO09_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0263517.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            15120..16928
                     /gene="uidA"
                     /locus_tag="IWO09_000906"
     CDS             15120..16928
                     /gene="uidA"
                     /locus_tag="IWO09_000906"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0263518.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            17100..17501
                     /locus_tag="IWO09_000907"
     CDS             17100..17501
                     /locus_tag="IWO09_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0263519.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            17495..17740
                     /locus_tag="IWO09_000908"
     CDS             17495..17740
                     /locus_tag="IWO09_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0263520.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(18064..18285)
                     /locus_tag="IWO09_000909"
     CDS             complement(18064..18285)
                     /locus_tag="IWO09_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263521.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(18447..18519)
                     /locus_tag="IWO09_000910"
     tRNA            complement(18447..18519)
                     /locus_tag="IWO09_000910"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18484..18486),aa:Lys,seq:ctt)
     gene            18595..18825
                     /locus_tag="IWO09_000911"
     CDS             18595..18825
                     /locus_tag="IWO09_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0263522.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            18869..20038
                     /locus_tag="IWO09_000912"
     CDS             18869..20038
                     /locus_tag="IWO09_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0263523.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            20612..21181
                     /locus_tag="IWO09_000913"
     CDS             20612..21181
                     /locus_tag="IWO09_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0263524.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            21168..22028
                     /locus_tag="IWO09_000914"
     CDS             21168..22028
                     /locus_tag="IWO09_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0263525.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            22218..22922
                     /locus_tag="IWO09_000915"
     CDS             22218..22922
                     /locus_tag="IWO09_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0263526.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            22927..24762
                     /gene="walK"
                     /locus_tag="IWO09_000916"
     CDS             22927..24762
                     /gene="walK"
                     /locus_tag="IWO09_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0263527.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            24759..26075
                     /locus_tag="IWO09_000917"
     CDS             24759..26075
                     /locus_tag="IWO09_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263528.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            26076..26945
                     /locus_tag="IWO09_000918"
     CDS             26076..26945
                     /locus_tag="IWO09_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263529.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            27000..27809
                     /locus_tag="IWO09_000919"
     CDS             27000..27809
                     /locus_tag="IWO09_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0263530.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(27912..28541)
                     /locus_tag="IWO09_000920"
     CDS             complement(27912..28541)
                     /locus_tag="IWO09_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ0263531.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            28711..30003
                     /locus_tag="IWO09_000921"
     CDS             28711..30003
                     /locus_tag="IWO09_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0263532.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(30079..30741)
                     /locus_tag="IWO09_000922"
     CDS             complement(30079..30741)
                     /locus_tag="IWO09_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0263533.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            30966..31250
                     /locus_tag="IWO09_000923"
     CDS             30966..31250
                     /locus_tag="IWO09_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0263534.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            31301..32926
                     /gene="groL"
                     /locus_tag="IWO09_000924"
     CDS             31301..32926
                     /gene="groL"
                     /locus_tag="IWO09_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0263535.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(33015..34163)
                     /locus_tag="IWO09_000925"
     CDS             complement(33015..34163)
                     /locus_tag="IWO09_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0263536.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(34265..34849)
                     /locus_tag="IWO09_000926"
     CDS             complement(34265..34849)
                     /locus_tag="IWO09_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0263537.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(34976..35407)
                     /locus_tag="IWO09_000927"
     CDS             complement(34976..35407)
                     /locus_tag="IWO09_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0263538.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(35425..35745)
                     /locus_tag="IWO09_000928"
     CDS             complement(35425..35745)
                     /locus_tag="IWO09_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0263539.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            36483..36740
                     /locus_tag="IWO09_000929"
     CDS             36483..36740
                     /locus_tag="IWO09_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAQ0263540.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(36711..37019)
                     /locus_tag="IWO09_000930"
     CDS             complement(36711..37019)
                     /locus_tag="IWO09_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263541.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            37097..37843
                     /locus_tag="IWO09_000931"
     CDS             37097..37843
                     /locus_tag="IWO09_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0263542.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            37858..38061
                     /locus_tag="IWO09_000932"
     CDS             37858..38061
                     /locus_tag="IWO09_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0263543.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            38166..38465
                     /locus_tag="IWO09_000933"
     CDS             38166..38465
                     /locus_tag="IWO09_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263544.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            38465..38647
                     /locus_tag="IWO09_000934"
     CDS             38465..38647
                     /locus_tag="IWO09_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263545.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(38653..39129)
                     /locus_tag="IWO09_000935"
     CDS             complement(38653..39129)
                     /locus_tag="IWO09_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263546.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            39182..39907
                     /locus_tag="IWO09_000936"
     CDS             39182..39907
                     /locus_tag="IWO09_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0263547.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            39894..40085
                     /locus_tag="IWO09_000937"
     CDS             39894..40085
                     /locus_tag="IWO09_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263548.1"
                     /translation="MKMNRAEALRIGTVIANRWWRHNKPNILSQQHIEKQKAWQQIKA
                     LYFLMLVDDTSIFFIFQNI"
     gene            40148..41440
                     /locus_tag="IWO09_000938"
     CDS             40148..41440
                     /locus_tag="IWO09_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0263549.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT        14268 a         6389 c         8185 g        12631 t
ORIGIN      
        1 atcatcaagg aaatagtaaa gaattagaag ttgtccttag atataattta ttttcgttta
       61 cgagtgaatt ttacttttta agacataaag agagaattgt aggaatggtt tctataattg
      121 ttaatccatt tcaaaactta tcctacagta tagtaaatta cctcatatgt gagcctgcat
      181 atactacgac tattttgaca acagtattag aaaatattaa cgaagtttca attttagaag
      241 taaagaagct agtgattcat ttgctaaatt caacagaaga tgaatcacta atcccctatt
      301 taaaagattc acattttttt aaagaagcgg tactatctaa agaatataga gagcatgatg
      361 ttttgagata cgcttacatt tattaaataa aatttctgat agtattttaa taattcggat
      421 catatcataa gatataggag ggaaaagaag tgattttatt gattttgtct ctcgtattac
      481 tagttgtatc taatctaatg aaaaataatc ctcctacaaa aattaattct ttttatggct
      541 ttagaactaa aaagtcaatg aaaaatgaaa aaagttggca tttagcacaa aaattgttgg
      601 gggatttttc aaaaaaaaaa gttatcccag tatttttgat gggtattgtt tttttcagta
      661 tagagattta tagtttagct attttaaaaa atgaaaattt tttcgttagt gtattaatgg
      721 gtgaaagttt aatcctaata ctgattttgt gtttaattgt tcatgaagtg aataaacaat
      781 tgtgaattaa aaaaataaaa acctagtata atcactttaa taaattgttg gtagaaggtg
      841 atcaaaatgg gagtaaacga aatgatgaaa gtcagattga gtttttaaat gataccttcc
      901 tcagaacttc aaaacgaatt caaagattcg ttgacaaatc ttgagcaaga tcatttaaaa
      961 aaatattttc tcttcatttg acgaaaaatg tttagaaatc ttatataagt gaagataaag
     1021 taattggtgt aattaattgt agagaatagc ctttgagcaa ttgaagcgat acaattattt
     1081 agaaaatgaa cttcctccag catactacat gattccttac gggacgatgt taaagaagaa
     1141 agcaagaaat tttagaagta ttagtagata aactaatggg cttttctcaa tttaagggag
     1201 tagcccaaat gtttatcaaa tatggtgcag atacagtagc aaccatgttt acagaagaat
     1261 atcaagtaga ctattatgaa gtaatagata aatgtgtaca ttttgtttat gaaaaagtgt
     1321 ggaattttat gaattttgcg ataagtgaag aaatcttacg agtacaagaa aacagaacag
     1381 gaatatttac cagagacaga aagcttaatt tgattgcgca ttatatacgg aatcgctggt
     1441 catcaatacc aaattttaat tataaaaaat aattatgaac aatcttttat acaatataca
     1501 caataaatca agtagtattt atgaaaaaag acttcaaaaa cagaatgatt ttctatcagt
     1561 cttagaaatt atagagctaa aaattttggt cacgcgtaga aacgcgtttt ttttgttttt
     1621 ttgatagtaa agaacgtaca aaacaatgag aatttaaaag attgtactac cagttttttt
     1681 accttttcat ttgagtttag agtaagtaaa atattactat aaaaacacat attcttgaag
     1741 aaatcttttt tttgctgtat tcatttagat tatgatagag aaaatacgtg agagggggac
     1801 ctgggatgtg gctatctgat gaaaaaattc agtggcgtag ctatcagaag cgtatattga
     1861 ctaactttgc tacttattta aataacaatt cagtccatct agtggctcca ccaggggctg
     1921 gcaagacctt tttaggaatt gagatgatcc gacgtttgga ttgtaaaacc ttaattttgg
     1981 ttccttccct aatattgaag aaacagtggt taacaacaat cgaggaatat ttcttagtaa
     2041 atccagcaga tagttgctat ctttctgggg cgattgaaca tcctagtcat attacagtgg
     2101 aaacgtatca gaccatttac aaccgactga aggaggagcc aacttatggt gatcagctgg
     2161 agctgttagt tttttgatga agctcatcat ttaaaaaaaa gctggggtga attgttgatt
     2221 gacttgaaga aaaaacaacc gaatctgttg acgatttcct tgacagccac gcctccgatt
     2281 gatagctctc agacggagtg gaagcaatat attgagttaa aatggcctga ttgacgaaga
     2341 aattagcatt cccgagttgg taaaggagga ggttctatgt cctcatcagg attttctata
     2401 tttgattccg gctaatcagg aaactactca gcattatttt acctttttag agaaacaaaa
     2461 tgagctggtg gaagcgttaa tggaaagcca agagacggtg gattatttgc tttctttaga
     2521 atttgttcag catccgcttg agcattcgaa ggaaatttat gaaaattttg agctgttcac
     2581 tagtgcatta atttacttga atcatcataa ctatgaattg atacccgaac attggcagct
     2641 attaggcagt tctaagcagc aaggtaaaaa agcttcagtt ccatctttag gtaaaaaaga
     2701 gctgatcact atttatcagc agatgtttga agatcaaccg gaattgccta ttttttatca
     2761 attgaaaaaa gctggatgat tgtttgatga gtcgctgcat ttgtttccag agtatccaga
     2821 cactgttggt agtcaggcga ttcctgagaa aaagcaagca gtttgtcaaa ttttgttaaa
     2881 agaagaagtt cagctgaagc aagaactgac agcggtcatt ttgatggatc gtatttgcaa
     2941 ggaggtatta gcaggcagtg atggtacctt agcttatggc gtggttcctt tgtttgatga
     3001 agttgttcgt ctattaggta acactcagca aatcggtgcc atctgtggag agttcttact
     3061 gttaccgaaa atgctagtaa ctgttgagct ggcagaacta gcgacgggta tgaatgaaca
     3121 tccttatatg tcgggttatt tttatttgac ggtggatgac aaaaaccgcc aagcattgat
     3181 gcaagcagtt actcgcctat taaccgaaaa gaagttgaat attttggtag gaaccgttag
     3241 tttgctagga gaaggctggg attgtcctgc aattaatctg ttgatcttgg ctaatcaagc
     3301 ttccactttt gtgcaaacac aacaactacg agggcggggc ttacgcctag gtgcacaaaa
     3361 taaagtaacg aacatttggc atttagcgat tggattaccg ggagttcctc tgtctgaaca
     3421 gcctgatttg aaaagaatgc tacggcgttt gggcaatatt tcaggtctga cctttgaatc
     3481 ttcaccaaaa attgagagtg gtggcgatcg ctttgcttta ccactggaat atacgaccac
     3541 cacgatggaa acttatactc agtcgatgtt ctatttttct tgtgaacgaa gtcggttgaa
     3601 agaacaatgg agtactgctt tagctgatgg ctggcaccta tctatgccaa tcgtcgttag
     3661 gaaagtcgat caggtaaagc aaaaacaaga aacttcattg ccgatagtag taaaaaatat
     3721 agagcctcta agcttctggt cagctatatt taaaggtaac ttgctggaat atttccaaat
     3781 tcgtaggcga caaaagctat gggaaaagga atgtgcccgc aaaaaaagct tggccatatg
     3841 cgttcaaacg acgttgcagc gccttggtga agttagtgat agaaaagtaa tcgtagcggc
     3901 tgatcagcag gaatttagat gctatcttga gaatgctggc tatcgagaag gacagctttt
     3961 tactgaagct atgagggagc ttttagcacc gctggagaat ccgcggtatt taatcgaagg
     4021 gaaaaagata gttctgacgg taccagaacg ctttggccga aataagcaaa tcgctaacct
     4081 ttttctaaac gaattaaatc aatcgtcgct gcccaaatgt cagttggtgt atactagaaa
     4141 tcctgaagga cgccaaaagc tagtgactta tcgaatgagg caagtcttcc agacccgcga
     4201 tgaagtaatt attcaaaaaa acatttggac gtaagttgat gaaaataaat tttgctagag
     4261 tagaacaagt ccgagaatta agttgctact taactgataa tgccaaggac aggagaatct
     4321 tttcttgacg actgaaagaa actatttgac ttatataacg gtgtgtgatg aatatttgca
     4381 aggcattctc ttaaaaagta ctcgtaatag tttttttgaa aacaaaacag tactttatta
     4441 ataggactga cagagcgata taagaacatc tgcagacaga ggtgaatgct tttctcatga
     4501 tcgatgaaac aatggtactt cggatattct tgcttttagt ttgaaccgtt gtgattttct
     4561 ttgattccta aaagtagttt tatagaatga aaatcaagca tacggaatgc tcctttgatt
     4621 gtgttgtggt gattcaatct aatggagctt ctatatgttt ttttatttgc aaaaagaacc
     4681 gatagaatat ttttatcagt cttagaaatt atagagccaa gaaatttcaa gcacacataa
     4741 aaacgcgtgt tttatgcatt ttatgagcaa aaataactaa agaccattac gtaaaacata
     4801 ggtaaatcag cattcaatct caaattatgt gatcaaatgt tgatattatt tgattataag
     4861 ttttcttttg ctattatcag tctgcaaata aacagattat agagtatgaa tgtagatagt
     4921 tcaatctttg tttacgacct cacatgtttt tttactcaag agaaagtaat ataaagtgac
     4981 tatatttgac agaactttac ataaaaaaag tagttagttt tctgtagtaa tcagaaatgt
     5041 aaataaatta aaaaaaagaa atttagcagt tatatatttt taaaatattt tgggtgagta
     5101 gtcaatacaa cattttgttc atcaagcatt aaatgaagat gcaaggatgt taagaagtta
     5161 gcttcttcag ggtcttgcat ggtcaaggct ttaaatagtt gatgatgttg ttccataatt
     5221 ttttcccatt ttaatccttc cgtttttact cgtaaccaac gaaatcgttc caagtgcgta
     5281 ttattatctt ccaaccattg ccatacttct tcccgttctg caatttcaaa acaagcttta
     5341 tgaaataaat tatcattatg aaaaaaacta cgagcattct tttgactaac agctttttct
     5401 tgttctgcaa ttatagtttc taaaacttgt ttcttttgat tattcatctt ggcacaacac
     5461 tctagcataa tttgtcgttc taaatgttct ctgacaaaac gagcattttt tgctaaatct
     5521 aagtcgatta atgagatata atttccactt tgaggaattg tatcaacaag tccttgatga
     5581 cggagttgaa tcaatgcttc acgtattgga gtacgaccga tagaaagcgt ttcttccaat
     5641 tttttttctg atactttttt tccaggttct aagtcagcgt agataatttg ctgacgaata
     5701 gattgataag cttgattttg gagactagtt ttaattgtca tatttaatcc ctctcaaaat
     5761 ttcatttttg aatttgatat tttagttcta tgctcgttat ataatatcaa atttcaaagt
     5821 aacatactag ctattaaaaa aacaaaaaat actcaaataa gatcaaagag aaattaagct
     5881 ttaatttaag ttagttctaa aaaatatata ttggtgtgct tttactaaaa aaagacacaa
     5941 taattctgac atggtagaaa aaagaatatt tttagttttt tcttattgac agcgattaca
     6001 ttcgatttta tactacaatt gtaacggaaa ctaacatatc agtatggagg ggtaaatatg
     6061 gttttgatta aagactggca agattctatc gaaggattga acaaaatgca agttcaacta
     6121 ccgtatttta atgtcgaagc ggtcaaacaa gaaggtcaga aacatccagt gtggcttcat
     6181 tttggtggag gaaatcttta tcgaggattt catgcagaaa ttgctcaatg cttagcagat
     6241 caaaatgaat tagaatccgg agtagttgtg tgtgagacat ttgatgaaga agtagtagaa
     6301 aaagcttatc atgactacaa caatgatatt ttggagattg ttatgggtga agatggtgaa
     6361 ctcaaaaaaa gacttttagc agtaacagct gatagtttat attgcaatcc aaagaatata
     6421 acggactatc aaagagtggt taaatatttt gaagatccta cattacagtt tacaacgttt
     6481 actattactg aaaaaggata cagtttaaaa gattctaaag gacaattttt ttcggtagtg
     6541 gaagcagata taaaatctgg acctaccaat cctcaacata cgatgagtat tgtgacatcc
     6601 ttgttgtata gcaggtttaa tgctggagag ttaccgattg cgatggtttc tactgataat
     6661 ttctcacaga atggtcaacg ttttcaagat agcattttta tgattgcaga agcttggcaa
     6721 aaaaaaggat acgtttctga aaaatttatt gcttatttaa aggataaaga aaaagtgact
     6781 tttccttgga gtatgattga tagaattacg ccaaatcctt cggaaagtgt caaaatggat
     6841 ttattgaagg cgggtttcga agacttagat ataattcata ctgcgaaaaa taccaatatc
     6901 gctgctttta ccaatactga aaaagtccat tatctagtca ttgaagatag ttttccaaat
     6961 ggtcgaccta aactggaagc ggctggtgtg attatgacag atagagaaac agttgataaa
     7021 gcagatatca tgaaggttac gacatgttta aatccgctcc atacggcatt agcaataaca
     7081 ggttgtttgt tggggtataa atcaattcag gcagaaatgc aagatgatga tttggttggc
     7141 ttaattaaag ggataggtta tgtagaaggc ttaccagtag ttgaaagtcc aggcgttatt
     7201 gatccaaaac aatttattga tgaagtaatc aataaacgtt tgcctaatcc ctatattcca
     7261 gatacaccgc aacgaattgc aacagatact tctcaaaaag tagcgattcg ctatggtgaa
     7321 acaattaaaa agtatgtcga aaaagacgga aatgcagaaa atctaacatt tattcctatc
     7381 gctattgctg cttggttacg ttatttattg gcagtcgatg atcaaggaaa aaattttgtg
     7441 ccaagtcctg atcctttatt ggtagagttg caggaaaaat tctcagtatt gaaattaggt
     7501 gaacaagatc ctgcaaagat tcattgtgcg gtacaggaaa tcttagaaaa taaggaaata
     7561 tttggtttag atttatatca agttgagata ggaaagaaaa ttgaaaaaat cttttgcgaa
     7621 atgctagtag gtcctcaagc agttcgtaaa acaatcaata aatatataac agaagttgga
     7681 ggaaagtaac tatggaaatg acttttagat ggtatggggc aaatgaagaa aaaatctcac
     7741 tacaagaaat tcgtcagatc ccagcaatga aaggaattgt tggaacatta atggatatac
     7801 cagccggaga ggtctggccg agagaacgaa ttaaagcttt aaaagatgaa gtcgaagcca
     7861 atggccttac cttgaaagtt attgagagtg ttaatgttca tgatgatatc aaaattggtt
     7921 tgccaacacg agatcaatac atcgagaatt ataaacagac gattcgcaat ttggcagaat
     7981 ttgggattga ggttatctgt tataatttta tgccagtctt tgattgggta aaatcagatt
     8041 tggattatca tttagatgat gggtcttcta cgttagccta tatttcaaaa aatattccta
     8101 ctgatccaga ggaaattatt agaaaagtag aaaattctac taataatttt gaattgccgg
     8161 gttgggaacc tcatcggtta tcacaaatca agcaattatt agaagcctat aaagaagttg
     8221 atgaagcgaa attgcgagag aactttgcat attttctcaa agcaattatc ccgacatgtg
     8281 aagaagttgg gattaaaatg gcagttcatc cagatgatcc accatattct attttcggat
     8341 tacctcgtat tgttaaaaat cgtgacgatt tagaatggtt gtgtaatgta gtggattctc
     8401 cttctaatgg tattacgtta tgtactggaa gtattgcaga acgaccagat aatgatgtct
     8461 atgaaattct atctgaattt tgtcaacggg atcggattcc ttttgcacat gttcgtaata
     8521 tcaaatattt aagtgataat ggactcgatt tttatgaagc gccgcataaa tcagaatatg
     8581 gttctctaga tatgtataag atattaaaaa caatgtacga taatggattt aatggctata
     8641 ttcgtccaga tcatggtcgg atgatttggg gagaaaccgg tagacctggt tatgggttat
     8701 atgaccgagc tctaggtgca gtttatttaa atggtatttg ggaagcgctt gaaaaggggg
     8761 cgagtaaata attatataat ggagtctatt tagaagaaaa attttctctt ctaaaaacta
     8821 agggaaggaa aggagtgctt aaatatgttt ttagataaga actttttatt agagaatgaa
     8881 atagctaaaa gactttttca taaatttgca gaacaccaac caatcatcga ttatcattgt
     8941 catttagacc cagcatgttt gctagaaaat aagcaataca agaatttgac tcaggtatgg
     9001 ttaaatgata aaggagctgg agatcattat aaatggcgat tgatgcgagc taatgggata
     9061 gaagaagagt tgatcagtgg tgcaggtaat gattatgaaa aatttttagc ttttgtaaaa
     9121 acagtggaga aggcgccagg taatcctatt tttgaatggt ctcatttgga attacaacgt
     9181 tactttgata ttgatttgac aatttctgag aagaatgcac cagaaatttg gcgacgtgcc
     9241 aacgaaaaaa tccaaacgga ttcctatcgc ccaaaagaat tgctgaagat gatggatgtg
     9301 aaagccattt gtacaacgga tgatcctaca gatgacttgc gatatcatac tgagctttct
     9361 aaacaggata attctttcaa ggtattacct actttccgtc cagataaggt ctggagtatc
     9421 aaatcatcag gattttcgaa ctatatttta aaacttagtc aagcagcggg tataccaatc
     9481 aaaaacttta atgggttgaa gcaggcattg agccaacgag tggattattt tcatcaaatt
     9541 ggctgtcgat tagcagatca cggtttgaat acttattttt atgaaatagc agatgaaaaa
     9601 atactcgaca atctgttaat aaaagcttta gcaggaaaaa cggatttcca tttatcagaa
     9661 attgcacaat ttgttacagg tattcaatta catttgatgt ctgagtatac aagatatggt
     9721 tggacgatgc aaatgcattt aaatgcttta agaaataatt caagtatatt aaaggaacaa
     9781 attggagtag atgtcggtgg tgattcgatg ggggatcaag ctagtttgac acaaaatgtt
     9841 gtagcattgt tcgacactgc tgaaaagagt gggtgcttac caaaaacaat tttatattca
     9901 ttaaatccaa ccgattggct tccactagca acagcaatgc aaagcttcca agggggtatg
     9961 attcaaaaaa tccaattggg atgtgcttgg tggttcaatg atacagctga aggaatgcgt
    10021 caacagttga ctacgatggc gcaacaaagt ttgttgtcga attttacagg tatgttgact
    10081 gattctcgaa gttttctaag ctatcctcgc catgagtatt tccgtcgagt attatgtcaa
    10141 ttactaagcg agtgggttaa agcaggtcga attccagatg atgaaatgta tcttagtcag
    10201 atagtggaga atatttgtta tcgtaatgcg tataattatt ttaatttttt tgaaatcgaa
    10261 agtagaaaat aatacaaaaa ataaaagctt aaatttattt attgtaagtt agtacaacta
    10321 gctagagtat gtatccataa aagaaggaga tagatatgaa aaaagtagaa attttaagta
    10381 agttagaagc ggctggagtc attgcggttg tacgtggaaa aaataaagat gaggccatta
    10441 aagcaataaa tgcaattact gcgggtggaa ttaaagggat tgaactaact tttacagttc
    10501 cgaatgctga agaaataatc aaagaaattg ctaaaaaata tgctgatcaa aaagatgtaa
    10561 taattggagc cggtacagtt ttagatgcgt ttactgctcg tattgcaatt atggctggag
    10621 cagaatatat cgtaagtcca tcttttgatc gacaaacagc agagatttgt aatttatatc
    10681 aaattcctta tttaccaggt tgtatgacaa ttactgagat aaaggaggca cttaccgctg
    10741 gagtagaaat cattaaacta tttccaggtt ctgcttttgg tccatctgtt gtaaaaacgt
    10801 atcaagctcc attaccatat gtaaatataa tgcctactgg cggagtaagt ctagataact
    10861 tagaagagtg gtttgaagca ggcgttgttg cagttggtgt aggtgggaat ttgttggctc
    10921 ctgttgaaaa aggtgatttt gaagaagtaa ctcgaatagc tagtaaatat atagataagt
    10981 tttatgagat aaagaattat taggagaaaa aataaataat actataaaat gtaaaaaata
    11041 aatactaact ctttatactt cagagtatag tacgtaagtt ggcagttcaa cagaatgaaa
    11101 acgcttaaca ataggaagga gcgcaaatta tatggaaaga aaatttggtc taagagacaa
    11161 aataggatat atgtttggag attttgggaa tgatttcact ttcatttttg ctagttcttt
    11221 tttaatgatt ttttatacaa aagtattggg gataagcgga gctaccgtag gaacattatt
    11281 tttagtagca agatttattg atgcagttac agacataaca atggggcgta ttgtggatgg
    11341 atcaaaacca tctaaaactg gcaaatttaa accgtggatt ttacggatga gtggtccagt
    11401 cgctttagca agttttttaa tgtatcaaac atcaatggca tcagcttcta tgagtattaa
    11461 agttatatat atgtatgtaa catatatact atggggaagt attttttata catctattaa
    11521 tattccttac ggttcaatgg catcagctat cacttctgtt gcagaagaaa ggacagcgtt
    11581 atcaacattt cgtagtatag gagctacctt agcaggtatg ttcattggcg tgttaacacc
    11641 aatttttatt tatacaagag atagttctgg agctcaaatt gtacgtggtg gatcaacttt
    11701 tacaattgtg gcaggagtat tttcagtgtg tgcagttatt tgttacgtaa tatgttataa
    11761 attaactgta gaacgggtta aaagtgatga atcgcagtta aaagtttcat tgttgaaatc
    11821 tttaaaagga ttagctaaaa atagagcgtt gctttctata atagctgcag ctattttttt
    11881 gttgcttgct cagcttttaa caatgagtat gaacaattat gtatttccag attattataa
    11941 taatgcacag ggaatagttt taatgaactt tatcaatcct attttgatgt tggtacttgt
    12001 ttcacctgta agtttaattt tcagtaaaaa atacggtaaa aaagaattgg cgtcagtagg
    12061 gatgttcttc tctgctattg tatatttact tttatttttg ataaagccta gtaatatgta
    12121 tgtattctta gttcttactt ctgttggata tatggggtta ggtattttta atacagtcat
    12181 ttgggcgaat attactgatg taatcgatga tcaagaagtg aaatctggcc aaagagaaga
    12241 tggaactatt tatgcagtat attcttttgc ccgaaaaatt ggacaagctt tagctggcgg
    12301 tgtcggaggt tggactcttt ctataatagg gtatgattcg ttagcaaaag tacaaagtgg
    12361 agaagtttta caaaagctgt ataactcttc aacgttgatt ccagcagttt gtttctttgt
    12421 tgtaggattg attttatttt tcgtatatcc attatctaaa gaaaaagtat tagaaaatta
    12481 taatttgcta aatcaaaaac ataactagga gaaaaaacat gaacagttgg atattttgta
    12541 ttgattcaga tggttgtgtc atggatacaa tgacttataa acacaatctc tttttcgggc
    12601 caattgctgc agagattttt gatgttcatc atagggaaaa attcctacag gattggaata
    12661 atattaattt atattcagag actcgtggaa taaacagatt tttaggttta gttaaaacat
    12721 tagaaaaaaa tgatgtacca tcgattaatc gattaaagca ctgggtatct gtaacaaaag
    12781 aattgtctaa tccatctttg aagagggaaa tagaatactc tcagtcagag gatttattaa
    12841 aagctttaaa atggtcggaa gaagtcaatc gacagattag tcaagctaaa ggattagata
    12901 aacccttttt aagtgctaga gatactataa aagctctgaa aaaatatggg aagataatta
    12961 ttgtaagctc agctaataag gaagctattc aagaagaatg ggaaagacat gaattattat
    13021 ccttagttga tgaactgtgt tgtcaagata aaggaaaaaa agaagacatt attcgaagtg
    13081 taattgaaaa tggttgtgat ttggataaga tattaatgat tggtgattct cctggtgatc
    13141 tagaagctgc aaataagaat aaagttttct tttatcctat tttggttaat aaagaaaaag
    13201 aatcatggga aaacttaaga actgatattt tatttaaatg tttatcggga gagtatatag
    13261 atattgaaca aaaatatata gatacttttt ggaaaaatct tactaataag tagaggagaa
    13321 agaaaatgac tataaaagta gacttacgaa aaaaaccata tcatttagat gaagatgcta
    13381 tttcttggat tgaagagacg attgatgcta tgactttaga agaaaaaata ggtcaattat
    13441 ttgttaatat gggatctagt cgtgataaaa attacttaag tagtatggtc aatcagtacc
    13501 atattggagc tgtacgatat aatccaggaa ctgctgatga ggtatatgat caaaactata
    13561 tcctacaaac agaaagcaaa attcctttat taattgctgc taatacagaa gcaggtggta
    13621 acggagcttg tacagatggt accgaagttg gtgtagaagt aaaaattggt gcaacaaatg
    13681 atgcgaaata tgcttatgaa ttagggagag tttctggaat tgaagcatcg gctattggat
    13741 gtaactggag ttttgctcct attgtagata ttaatagaaa ttggagaaat cctatcattt
    13801 ctagtcgatc ttttggacaa agccctgata aagttttaga acttagttta gcttatatga
    13861 aaggtattca agagagtgga attttgcctg cagctaaaca tttccctggt gacggtattg
    13921 acgaacgaga tcaacatcta tcttttagta ttaatagttt aagtacagaa gaatgggata
    13981 attcttttgg aaaagtatac tcaggcttaa tagaagctgg attaccttca attatggcag
    14041 gtcatatcca tatgccagct tattcaaaac attttaatct tgatctgaaa gatgaagatt
    14101 tacttcctgc aactttatca aaagaattaa tcactgactt acttagagga aaactcgggt
    14161 ttaatggatt agtagttact gatgctagtc atatgttagg aatgactggg gcaatgaaac
    14221 ggtcagaatt attgcccact tctattgcgg ctggatgtga tctattccta ttttttaatg
    14281 atccagatga agactttaat tatatgatgg aaggttatta caaaggaatt atttcagaag
    14341 agcgtttacg tgatgcatta agaagaattt taggcttaaa ggctaaacta ggtttgcata
    14401 aaaaagaaaa gaataaaatt ttgttaccga aacaagaagc tatggaaaaa attggtttaa
    14461 ttgaaaataa atcaattttt aaagaggtag ctgatcaagc aattacacta gttaaagata
    14521 aacaagatat ttggcctatt gtaccagaaa aatataaaag gattttactg gtagatgtac
    14581 atggggtaaa aggtgggttt ggtgaagtag ttgctggaaa tagacctcag gccattgatt
    14641 tattgaaaca gattttgtca gcaaaaggat ttgaagttga aaaatacatt tcacctgaag
    14701 aagaattgaa aggattatcg caagaagaaa aaattaaaaa tatagctgga atatatgctg
    14761 ctaagagacc aatcaaagat ttaactgaaa aatatgactt gattattaat atagctaatg
    14821 ttaacccagg tacagttcaa agaattgttt ggccagcaag taaaggtact ccggatattc
    14881 cattttatat tcatgaaatt ccaacaattt ttgtatctgt acagtgtccg tttcatttag
    14941 ctgatgtgcc tcaagtaaaa acttatatca atacttatga tggaaaacaa gaaactttag
    15001 aaatattagt ggacaaacta atgggccttt ctgaatttaa gggaataagt ccaatagatg
    15061 cttattgtgg gttaaaggat acatttattt aatatttaaa ttatatagga gtgatttaca
    15121 tgttatatcc cattatgaca gagacgaggc aagtaattga tttaagcggt ctttggagat
    15181 ttaagttaga tacaaatgat gacttacaag aagaacttgc tttagaacca atggttgaaa
    15241 aagagagtta tccaatttca gttccttctt cttataatga tttatttgaa acagagaaca
    15301 ttagaaatca tgttggatgg gtgtggtatg aaaaagaagt gattcttcct aaaaaattat
    15361 tagaagaaag attagttctt cgttttggat ctgcaacaca tgaagcaaaa gtatttttga
    15421 atggaaaatt gattaatcaa cacaaaggag ggtttacgcc atttgaatca gaaattaatc
    15481 agtttataaa agatggaagt aacagaatta cagtggctgt aaacaatgtt attgacgata
    15541 gcactttacc tgtagggatt ttgaaagaaa cgatacagga agatggagac ataaaaaaag
    15601 aaaatttggt taattttgac tttttcaatt atgcaggcat taatagaccc gttaaattgt
    15661 atacaactcc gaaagactat ataaaggatg tcactattaa tacaaaaata attgattcag
    15721 atgcaaatgt agaatttaat atcgatacag aatggaccgg agaaattcta atagcaatct
    15781 atgacgaaga agctcaactt atagcagaag ctaaggggaa agaaggagtg attcaactta
    15841 agaacccaac actttgggag cctctaaatg cttacttata tagtatgaaa gttagtttaa
    15901 ttaacgaaaa taaagtgatt gatacgtaca cagaagaatt tggcattcgt agtattcagg
    15961 tttcgaatgg gaagtttttg attaataata aaccttttta ctttaaggga tttgggaagc
    16021 atgaggacac gtatattaat ggtcgtggtt tgaatgaagc agtaaatatc aaagatttta
    16081 atttaatgaa atggatcggt gctaattcat ttaggacttc acattatcct tattcagaag
    16141 aaattatgcg cttagcagat cgagaaggta ttgtagttat tgatgaagta cctgcagtag
    16201 gattacattt gaatttcatg gctactttat tagatgacaa cgtagaaaaa catgatactt
    16261 ggaaagaaat acggacagaa aatgctcaca aaatggcgat taaggaattg atagaacgcg
    16321 acaagaacca tccgagtgtc gtaatgtggt ctattgcaaa tgaaccagat tcggattctg
    16381 aaggcgctaa agaatatttt gaacctttag ttgaattgac taaagaactt gaccctcaga
    16441 agagacctgt aacaatcgtt acttacttaa agtcaacacc agatatttgt aaagtaggag
    16501 atattgtcga tgttttatgt ttaaatcgct attatggttg gtatgtttca ggtggagacc
    16561 taaaacatgc aaaatatctg ttgaaaaaag agctagatgg atggatggaa agatgtcccg
    16621 atacgccaat aatgtttaca gaatatggag cagatacagt agctggactt catgatacag
    16681 taccaacaat gtttacagaa gagtatcaag tgggttatta cgaagcaaat cacgaagtaa
    16741 tagataaatg tgacaatttt gttggtgaac aagtgtggaa ttttgcagat tttgaaacaa
    16801 gtcaagggat tttaagagtg caaggaaata agaaaggaat ctttactaga gatagaaagc
    16861 ctaaaatgat tgctcactat ctaaagaaac gatggtcatc aataccaaat ttcagttata
    16921 aaaaataatt taccttttat aaggaatata aaaaaggagt ggctgcttaa ttgccaactc
    16981 ctttttttta taaaaagatt ttctgtttaa agaaggatat ttttttctag ttttttcgaa
    17041 aaaaaggcgc tttctgattg ttgtattagt ggaaagggga aaaaaggagc atatttatca
    17101 tgcaacaaaa ttacaatgag aaatctatac aacatcaatt tgatgcctac tgtaaaaaag
    17161 tattacgaaa cgaaactaag tctattcgaa aacgaaacat taacataaca gataatgagg
    17221 aaccattgac gaattcaaat gaaggtaagt actcccaaac tcattttgat gaacaagatg
    17281 tctatttctt atttggaatg gaaatattaa tttcagatca aaagttatcg gcagccattg
    17341 atcatttatc ggaaataaga agaaaggtta tcctacttta ttattttgca ggttttaatg
    17401 atactgaaat cggtaaaatc ctcaatatga gtacaagtgg tatctggtat caacgaaaga
    17461 aggcagttga acaattaaaa atggggtatg acttatggta gacaacccca aaaaacggca
    17521 gtatccatta gttcctctat ccattatcca atctgctcta gaagaagatc caatttcaat
    17581 caagatgata ctcaaacatt atcatagtta tatagctaag ttatgtttga caaatggatt
    17641 taatgaggcg gggcaattta ttacctatgt agatgagtat atgctaagac agctagaaat
    17701 taaattgata gaagcgatct taaaatttaa aattaactaa ctaaaaagtc ccctttccac
    17761 ttattctgtt taaaagaatc ttctaatagt taaaggatta gtagattctt ttaaacagaa
    17821 aacctattga tcattgaaat ttaaatacat tctatcagta cgttaacact tcaaggagca
    17881 tattttagtt gcgccatgat ttcttactgt aaagaaggag cgataaacat tctaaaataa
    17941 tttttatagt caataaaaag cgatgatttg aagttgggat aatgatactt ccgtaaggct
    18001 cgccgtaaac ttggagagag gtgaaattcc tatgtgcttt tgacaaaagc agctgattaa
    18061 gaattatttc catatgttga tttcaacatt ttttctatta tttttaagac caaattcagt
    18121 gaggcaaata gcatcatgta aatttagttt ttctattgaa cgttctccac tttttagttt
    18181 ttttatagta ctcaaattta atcctgtatg tttagagatc tcatttgcag tatggttttg
    18241 taaagtttct cggattattg ttatattagc aatttcatta cgcataatta tgttcctttc
    18301 ttatcaaaaa tatcaatgac tacagtgggc ttttagtgaa aaaaaggggt actacagtct
    18361 cttttgaaaa caaaaaaata cagtatatac gttttctaaa tgctaagcac caacgtttac
    18421 actgtattta agggtttggc acccaatggg ctgtgagggg ctcgaacccg cgacccgatg
    18481 attaagagtc atctgctcta ccaactgagc taacagccca aattaatact ttgttattct
    18541 aacacctaaa ataaaaaagt gcaatactta ttatcagaat tgagagtgaa ttttatggat
    18601 tttcaagatt atgaaaaaat atttgaaagt tatccatcga tattgaaaat tactgattta
    18661 actaaaatgt taaatattgg aaaaaataaa gcttataatt tgctgaaaga tagatcaata
    18721 gaaaatataa aaattggagc aaggtacaaa atacctaaaa aagctgtcat tgagtatata
    18781 ttgaaaatac aagatgaagg taaaaatcat gctataataa aatagtcaaa atttttgccg
    18841 atatttcagt ttaagaggag agattgaatt gaaattaaga gcaaacataa ttgaaaaaag
    18901 aggaacatta caagtagttg tatcttataa agactctaac ggaaaaatta gacaaaagtg
    18961 gaaagataca aaattaaaaa agaaaggaaa taaaaaacga gcagaaaaaa ttagagatga
    19021 atttttacta gaattggaaa gtcaattagc tatggaatgt gaatgtgcta atccaaattt
    19081 attgttttat gactatttgc ttaattattt ggaagtatca aaaaaacaag tagcatttaa
    19141 cacatatgtt gggtataagc attacgtcca taatcgaatt tacaagtttt tttatcctaa
    19201 aaaaattaaa ctaaataggt taaagcctta tcatttacaa gagttttatc aatatatgtt
    19261 aaatgataac tgtacagcaa atacggttat tcattatcac gcatttatac gcaaagctct
    19321 tcaagaggct gttattacag aattgataag tacaaatgta gcagataaag taagaaagcc
    19381 aaaaaagaaa caatttgttt ctcaagttta taatcaacaa gagatcttga aattacttga
    19441 cattttgtca gaagaaaaac tctatttggt agttctttta acagcatttt atggattgcg
    19501 aagaagtgaa gttttaggat taaaatggtc tgctattgat tttttaaaca aaaaaatcat
    19561 gataaaccat gtaatcatag aaaatccaga gaatatgtca cagttaataa agaaagatca
    19621 aacaaagaac tcttccagct atcgtacatt acctttggtt gaaacgattg aacgagcatt
    19681 agtaaatcaa gctaaatggc aacaagataa tcgtattctt ttaggagatg attatatttt
    19741 agaagataat gagtacgtat ttactatgga agatggacgg ttgatgaagc ctcaatatgt
    19801 aacgcatcgt ttgagtaagt tgattaagaa aaatggattg aaaaaaattc gctttcatga
    19861 cttgcgtcat tctaatgctt caataatgtt agatagtggc cagaatatga aatccattca
    19921 agaatggctg ggacatgcat cgtattcgac aactgctaat ttatacacgc atttaaccgc
    19981 aggagtaaaa gatcgtgcag cagaagcatt agaaaacgca tttgggttta aagaataaaa
    20041 aaacaaaaaa caatatcttt ggggtatcgc ttcttgtttt tagaattttt ttgttggcaa
    20101 tttgttggca gtatgttggc aatgaaaaaa ttcacaaact aaaaacctat ttataaagct
    20161 ttataaaact tttttcacaa atagagcatt tggttaagag tcagctgctc taccaactga
    20221 gctaacggcc cgtacaaaaa gtacttaata atagtagcac taagaatttt aaaatgcaag
    20281 actacattta aagaaaatca gaaaaatctt tacttcctta taggaaatca ttcatagatt
    20341 attcacattt gctcgctatt atataagcat accaacaaac aaagttcttg caagagaaca
    20401 aaccctaaca cttttttcat tttttactcc tttcccgccg tctccccggc gggtctcctg
    20461 ttcttaacga taaaagacaa gaaggcttag ccttcttgtt ttttgttttc atggaatccc
    20521 gctaatcaaa tcacctagtt aaagcaacta gattgaatgt ttatcataag tctgatatca
    20581 tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa atgcgtcagc aattaatgga
    20641 gtgggggaag gaactagaaa cattccgtct gcctcactgg gaagaactcc ctgacttaga
    20701 actttatatg gaccaagtca gaacgttagt tgatcgttat ctttcacctg tgatacaagg
    20761 agaaaagcat ccattgctta cctcttctat ggtaaacaat tacgttaaac taggtttgat
    20821 accagcacca gttaaaaaaa gatataacaa agaacacgta gcatttttac ttgcaatcac
    20881 tacgttgaaa caagtattga ctattcctga aatcaaagaa gggatattgt tccaaggaaa
    20941 aacagtagga attcgtgaag cctataatct attttgcgat gaacaagaag cagctgtgtg
    21001 gatggtcagc caattagcac aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc
    21061 tgtagaatat attgctgtaa gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga
    21121 aaaaacgatt gttctagaaa ccgaatattt aaaagaggag aaaacaaatg aaaaacaata
    21181 aaatcgcaat tttagtagat tcaggtacgg atgttccaga agaattgata aaaaaatacg
    21241 agatgtatat gatcccattg aaaattattt ataaagatca ggtatataca gataaaattg
    21301 acattacccc tgaagagatc tatcaacgtt tgccagagga aatccctggt acatctttgc
    21361 cggatggtga gtcgatcaca gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg
    21421 tacttgcggt aacaatttcc agtggattga gcgggacata taatgtggtt cgtttaattg
    21481 cacaacagca agaagaattg gaagcatatg tactagatac gaaaaatatt ggtattggtg
    21541 caggtttttc tgcgatccaa gcagcaaaat ggctggaaga aggagtagaa tgggatcaac
    21601 tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat
    21661 tggaatatct tcaaaaaggt ggacggatcg gtttagttgc ttctattgta ggaaccgctt
    21721 taaagcttaa cccaatcatc tcatgtaatg aagaagggat ttattacaca gtaggaaaag
    21781 ctaggggacg taaaaaaagt ttggataaaa cggttgctta cgtcaaagaa aaaatcggta
    21841 atgcaaaggt cttcaactta gctgttgccc atggggatgc gaaagaagaa gcaattgaaa
    21901 tggaagcgag acttaaaaga gagttcccac aagcaaacca aatctacttt ggccagattt
    21961 ctccggcatt agtagtccac actggtccgg gattattggg tgtcggtgta caagttttag
    22021 aaggataaat caaaaaaagg aaacactgag gtgaaaatca gtgttttcct tttttattga
    22081 agttagaaca aaaaataata aagtattttg cactttcatt ttaattttct cttttgtttt
    22141 ataggcaaag atgaaacaaa agcagaaata cggtataatg agaaggtatg acaataatat
    22201 ataagaggtg gctctttatg aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg
    22261 atatcgtcaa attcaattta gccaaagaag gctatgacgt ttatactgca tatgatgggg
    22321 aagaagcctt ggaaaaagtt gctgaggtag aacctgattt gattttactt gatttgatgc
    22381 tacctaaaat ggacggctta gaagttgctc gtgaggtacg caaaacttat gatatgccga
    22441 tcattatggt aacagccaaa gattctgaaa ttgataaagt cctaggctta gagttaggcg
    22501 ccgatgatta cgtgaccaag ccattttcca accgagaatt agttgcacgt gtcaaagcaa
    22561 atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga ggcagcacct gcggaattga
    22621 cgattggtga tttgacgatc catccagaag cttacatggt gacgaaacga ggagaaacaa
    22681 tcgagttaac acatcgtgaa ttcgaactac tcttttactt ggcgaagcac cttggacaag
    22741 tgatgacaag agaacattta ttacaaacgg tttggggtta tgattatttt ggagatgtac
    22801 gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc
    22861 caaattattt agtgacacgt cgtggtgtcg gttactattt gcgtaaccca gaacaggagt
    22921 aattggatga aaagaaaagt ccgctttttt cgatcagtca attttaagat cgctattacc
    22981 tttattctga ttttattgat ctcgatcgaa attattggtg cctactttat acgtggttta
    23041 gaacgttcca cgatcaatac atttattaaa gatatgaatc aaacggtgga atcattagca
    23101 acaacaatca gcccggaatt gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg
    23161 aatatcaaaa gatttattga aaacagtgcg actagcgata tcatcgaaat acgggtcgtt
    23221 gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag
    23281 aaaaatgatt atgttgatat caatgatttc acgactaaac gttatgttgc tttagataac
    23341 gacgataaac gggtgaatat caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt
    23401 atcggggctc tttacgtcaa aagtaatatc gaacaaaaat attcggaaat caataacaca
    23461 gcagtcattt tctttactgc ctcactaatt gcggcattta tttcaatgat cgtatctgtt
    23521 ttagttgctc gttcgattac acagccgatc ggtgaaatga gagaacaagc cattcgaatc
    23581 gctagaggag actacagtcg gaaagtcaaa gtgcatggac aagatgaact tggacagctg
    23641 gctgatacct tcaatcagtt agctgaacgt attgaagaag ctcaagatac aatggaagct
    23701 gaacgcaatc gtttggatag cgtgctatcc catatgacag acggcgtcat tgcgacagat
    23761 cgcaggggta aagtcattac gatcaatgat atggctgttt ccttgctaga tatcaaaaac
    23821 gaggaagcaa tcggacaatc gattttgaca cttttagata tcgaagaaga atatacgttg
    23881 cggaaattat tagaagatcc aaacgaaatg gtacttgacc gttcaaccag tacattggaa
    23941 agtgatcaga tgatcttgcg agtagacttt tctatgatcc gtcgcgaatc aggttttatc
    24001 agcggattag tagctgtctt gcatgatgtg acagaacaag aaaaaacaga acgggaacgt
    24061 cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt
    24121 tatatcgaag cattgagcga aggtgcgtgg aaagacgagg aagtagcacc aaattttttg
    24181 aaggtaacgc tagatgaaac agaccgaatg attcgaatga tcaatgacct cttaaatcta
    24241 tctcgcatgg atacaggtaa tacccagctt caattagaat atgtcaattt caacgagtta
    24301 gtgaattttg ttcttgaccg gtttgatatg atggtgggaa atcaggaaaa aaattataag
    24361 atccgtcgtg aatttacgca aagagatctt tgggtcgaag tagatacaga taaaatcatc
    24421 caagtagtcg ataatatcat gaataacgcg atcaagtatt caccagatgg gggaacgatt
    24481 acttgtcgct tattagaaac gcataataac gtgattttaa gtatcaccga tcaaggattg
    24541 gggattccta aaaaagatct gaaccgagtg ttcgagcggt tctatcgagt agataaagca
    24601 cgtgcaagag cacaaggtgg gacaggactt ggattagcta tttctagaga agtgattaag
    24661 gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa aagggtcgac tttttatatc
    24721 tcgctgccat atgaacctta tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg
    24781 ttcgtatagg actcattttg atggttgcgt taagcatata cttctcttat gcaatctggc
    24841 tgagcccagc aggaaaaagt tcaatcaacg tagatgagac aaacactcaa atgatcgagt
    24901 ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct tcatgttaca tggcaccatt
    24961 cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg
    25021 ataaagcaag atttggaaag ctttctgaag tagtttcagg agacaaatca cagtttgaac
    25081 aggtgaaaaa tctgaactct ggttttgagt tggcctataa tgcgcctttt ttgctaagag
    25141 aatacaagga agcatttgat ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa
    25201 atatgtattt tactagaata caattcgatc aaggaaataa aaaaatccga tttttgaatt
    25261 attcgaacca tctcgtatat gaagcaaata tttcgttgaa ttggacagat atcaataaag
    25321 aattaacagc aaaagacaca aaatggacag aaatgacaac agaaccatcg acgatcgaca
    25381 cgcaatatgc tacgaaacat tcgatcaaac tgaagaagta tagttatatc ctgtcacagc
    25441 aaccctatac actttttaga aatgcttttt tccagaaacc agatagcgtg aaaaataatg
    25501 atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac aatgtctatc cattcggaaa
    25561 cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa agaaaatgct agtgtatttt
    25621 ctgaaagttt cccctatatt agcaaattag gcagttcttt agggaacctc agatattttg
    25681 accgcagtga caatacgatc gattatcgga tattcgtgga aggcttcccg gtttttggga
    25741 cagacagcag aggacagatt ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag
    25801 tgaatatcca aaccagtttg aatacgattc aagtgccgat accttcagac gaagaagtag
    25861 aacttgcaag tacgaatgaa atcatccagc agttaaaagc taagggagca gatagcaaca
    25921 agattggttc agtgatcgtt ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg
    25981 ttgatctctt acctgaatgg tatgtgaaat ataaagataa ctggtattca gctaacgacc
    26041 ttctggaaaa attacctgga ttggaggcga attaattgga tttcaaaaaa atcgaatgga
    26101 tatttttcgt cgctttttta gggttgaatg tgtttttatt cagcagttat catgacgcgg
    26161 tataccaaga aaacaatgtc attcgctcta atcagactga atcaatccaa caacgtctgg
    26221 ctagcgacga tatcacttac acggggaaga tttcgtctga gaacaagcaa ggctattatc
    26281 ttagcgcgga gcagaccaat cttagcactt cattagcgaa ttatcgcaag gctggtaaaa
    26341 gcggcttgct ggcaagtggt gtgtttttag acggtgatgt tttgacgaaa tctttatcca
    26401 gttcagatgc tgaaaagaac gtgatcgatc cagataaaat tagtagttcc ttggaagatt
    26461 ttttatctaa caaagacaat attttattcg gtaatgatta tgtgtacacg aaaaactttt
    26521 ctcatttaga agaagaacca ctagaaatcc aagcaagcca agagtatgaa ggcatcccat
    26581 tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa aaacgataat gattatagca
    26641 tcacgaaata catgcagacg catttatctg atatcgagca gctgcgcgaa aagacggaac
    26701 tgtatacgga agaggaagca atcaatacat tgtacatcaa taacaaaata ccaagaggat
    26761 caaaaatttt atggcgtcag ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg
    26821 tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga taaagtggtc caaattgaga
    26881 gcgtcaacgc tttcagcaat acgatcgtga caaacaatac tgtaccaaaa gtggaagatc
    26941 aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa
    27001 tggctgaaga aaaaccagct tttaaaatca gtatcctagc aagtggaagt tcgggtaatt
    27061 cactttatat cgaaagtgaa aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa
    27121 aaatcacttc cttgatggct caaattgatc ggacaccgga ccaattagat ggaatacttg
    27181 tgacgcatga acatcgagat catatacatg gcgtgggtgt cttggctcgt aagtatcatt
    27241 tggatgtttt tgccaatcag cagacttggg aagcgatgga accactgatt ggaaatgtac
    27301 cagtggaaca aaaacatcta tttgagatgg ggaaagtcag aacattcggc gatatcgata
    27361 ttgagagttt cggcgtctcc catgacgcag cggctccgca attttatcgt ttccataaag
    27421 aaaatcggtc attcgtcatg ttgacggaca ccggatattg cagtgattat atgcgaggtg
    27481 tcattgaaaa tgccgatggt tatttgatgg aaagtaatca tgatcttgag atgttacgca
    27541 tgggacctta tccgtggaat ttaaaacagc gtattttagg agatagagga catctttcca
    27601 acgaagacgg tgcacttgtc atgactgatg tgatcggtaa tcggaccaaa cgaatctatc
    27661 ttggccatct tagcaaggaa aacaatatga aagaacttgc tcatttaaca atggaaaatg
    27721 ttttgaaaga aaaagatttt ggcgttggac atgattttga aatctatgat acagatccag
    27781 atgaagcaac tgagctattt tcgatttaaa cagttcaatc aataaaaata taaaaagcag
    27841 aagataaaat caatcagggt tggttttatc ttctattttt atttttttag catattaaaa
    27901 aaataaccgt attatcgact gactacactg atgtgctgct tttcatgttt ctcatccagt
    27961 atctcatcta ctaatgcaat cgcgtaatct gccatgctga tcgtactgtc gctagcagcg
    28021 tttctttgta aataatcatc acttaacaaa taagtacctg tacgtttgcc atgtggatca
    28081 aataaagaag ctggactgat ataagtccaa tgtagatttt tagattgttt caattttaac
    28141 aatccttcgt acatattgaa agctgttgga tagaagggtg catttttagg tacttggtca
    28201 atcatacgag tcgtcttttc tgtatctaag aataacgaag aggcaccccc aacgacaatt
    28261 aagcgtgtat cggttccttc aagcatctca atcaaatggg acaaagaagt ttgatgcatt
    28321 tcttcttttc ctttgggagc gttgaaagca tcaacgacaa catcaaaaga tgcaagatca
    28381 gtagttgtca actcaaataa atccttttgt ataaacggaa catctgatgg caatttttct
    28441 ggatgacgca cgattgccgt cacgtctaaa tctctggaca atgcttcgtc taaaataaat
    28501 gaacctgcat ggccagtagc tccaatgagt gcaattttca taaaaaacct ccttttttat
    28561 tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc
    28621 agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag ttaaattaag aaaactgatt
    28681 gataaaaata gaagttgaag gagataagaa atgaaagagt ggttagatcg tgccttttta
    28741 ccaatgataa catctgttgt gaatcttaga cccttaactg ctttgcgtaa tggaatgact
    28801 tacacgatcc caataattat tgttggctcg ctgtttttct tatttgctca ttttcctgtg
    28861 gattacatcg ctcagtggat agacaaaaca ggattttcta tgtattttac acaagtatac
    28921 caggctacat ttgatgtgat agccttttgg atcgtttttg ccattgcata cgcttatgca
    28981 aaagaagaag acatcgatcg cttaccagtc ggcataacag ccttgtctgc ttatctactg
    29041 cttattcagc cactaaatac tcagatagat actgtctgga caggtgctga agggatacta
    29101 ggagcagtta caactgggct catcatcgga tggggctatg cacgtttagt aagacttgcg
    29161 aaagggtgga agcaaagaga gaagatacct gaaaatgttt ggcagtcttt tacttcattg
    29221 attccaatca ttgttatagt cacttttacg cttatcggtt cagcggtttt tcaacatttg
    29281 ttcagaatga ctgttgttca aggagtatgg acatatgtcc agcttcccat tcaatcgttc
    29341 atagacacgt tgtttggtgt gcttttatta ggtttttttg ttccgttttt atggttattt
    29401 ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc ccgtcttgca agcgaattca
    29461 ttagcaaatg cagaaatctt agcctcagga caagagttga cggtggcaaa tggcggacat
    29521 atcgttactc agcaatttct cgatcagttc atgacggtta cgggtgctgg attgacgtta
    29581 ggggctgtat tttttatgac ggtttttgcg aaatcgaaga aatacagaga attaggaaat
    29641 cttgctcttt taccagcctt tttcaacatc aatgaatcaa ttattttttc cacaccgatc
    29701 gtgatgaacc cgatgatggc tgttcctttt attttcgcac cggtcgtttc aggattgatt
    29761 acctattccg ctttatattt tggattcgtt cctttattta cagcagtcca agtcccttgg
    29821 acaacaccgc cgatcctctc gggtttctta gtaggcggag tgcctgcagc gattttacaa
    29881 ggtatcgtgt tgtcaattag ttttttcatt tatttcccat ttttgaaaaa aatagattct
    29941 gcaaactgca aaaaggaaag acagacaaaa aatgatggag cagcagaaaa gatgaacgac
    30001 taaaaaacaa aaatactcct gtcgctttag attgggaaaa tcaccaatct aggatgacag
    30061 gagtatttat agtcatgttt atactagccc tttttggaca gctaattgaa caagaacaac
    30121 taatgtgttc attcctacgt gtgtgatcat cgatgtccag atccgaccag ttgctttgta
    30181 ctctaggctg aagaagaaac ctaggaaaaa gtagattagc agatgaccat cattatgagc
    30241 aaacgcaaac agcaaagagc tgatcactgc tccgacccaa aaatttgaat atcgactgat
    30301 gatccccagt atactgcggc ggaatacgaa ctcttccatg atgggtccag caacagttgt
    30361 cgccaaaata aaagcgggtg cttccatgat catctggatg atattttgtg tattctcgga
    30421 aggggttgct ttgccaaaca atacagattc taacatgaca gcgatgcttt gcagaatcaa
    30481 tgcgacaaag attccaacca atccataaat aacaatcttt ggagcagaag ctttttttac
    30541 cgctgattcg atcgctaagg gttcacgttg cttaaaataa aaaatagcta ggacaacggc
    30601 gcctagtata tacatgactg cagttgctgt gatcaattca gacgtagatt ttacaagtcc
    30661 tatcgaagct aggatcaagg gtgagataaa aattagacca tagcaaaaaa tgctgaggaa
    30721 gctatatttt tttaaagaca tgtaattcct ccttttaagg gataacaagc atgcaaggca
    30781 agcttatatc gattgatttc aattatatca taatttcaat aagcagctgt cagaataaaa
    30841 tttcttttct ttgatttaaa agcttgcaaa aaagagggag aatgagtatt attaaaaatg
    30901 taggttagca ctcgaaggtt aagagtgcta acagattaaa aaaagagtta tcggagggat
    30961 ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa
    31021 aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga gaaaccccaa actgggacag
    31081 ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga aaaagttcct gccgctgtaa
    31141 aagcaggcga ccaagttatg ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa
    31201 aagaatatct gatcgtcgca ggaaaagata tcatggcgat tgttgagtaa atttggaaga
    31261 taaaaaaata atcaaaaata atcaatgagg tgaatcaatc atggcaaaag aattgaaatt
    31321 tgcagaagac gcacgcgcag ctatgttacg cggggtagat aaattagcag atacagtgaa
    31381 agtaacatta ggacctaaag gccgaaatgt cgtactagaa aaatcttacg gctctccatt
    31441 gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa
    31501 catgggtgca aaacttgtat cagaagtagc atcaaaaaca aatgatattg caggagacgg
    31561 tacaacgaca gcaactgttc tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac
    31621 tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt
    31681 cgaagaattg cataatatct caactgttgt tgattcaaaa gaagccattg cacaagtagc
    31741 agctgtttct tcgggttctg ataaagttgg acatttgatt gctgatgcaa tggaaaaagt
    31801 tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg atcgaaacag aactagatgt
    31861 tgtagaaggt atgcagtttg atcgtggtta cttatcacaa tatatggtaa cagataacga
    31921 caaaatggaa gctgttctag aaaacccata tatcttgatc acagacaaaa aaatctctaa
    31981 catccaagat attctgccat tgttagaaca aatcttgcaa caatcacgtc cattgttgat
    32041 tattgctgac gatgtagatg gtgaagcatt gccaactctt gtattgaaca aaatccgtgg
    32101 aacattcaac gtagtagctg taaaagctcc tggtttcggc gatcgtcgta aagcaatgct
    32161 tgaagatatc gctattttga caggtggtac agtaattact gacgatctag gtttagaatt
    32221 gaaagatgca acaatcgaaa acttaggaaa tgcttctaaa gttgttgtag ataaagacaa
    32281 tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat
    32341 caaaaaccaa attgctgaaa caacttctga ttttgaccgc gaaaaattac aagaacgctt
    32401 agctaaatta gcaggtggtg tagctgttgt taaagttggt gcagctactg aaacagaatt
    32461 gaaagaatta aaattacgca ttgaagatgc attgaatgct acacgtgccg cagtagaaga
    32521 aggaatggtc tcaggtggtg gtacagctct agtgaacgtt atcagcaaag tttctgctgt
    32581 agaagcagaa ggagacgtgg caacaggtat caagatcgtt gttcgtgctt tagaagaacc
    32641 aattcgtcaa atcgctgaaa acgctggtta tgaaggatca gtgattgttg ataaattgaa
    32701 gaacgtggaa ctaggcactg gtttcaatgc tgctactgga gaatgggtaa acatggttga
    32761 agcaggtatc gtagacccaa ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt
    32821 ttctgccttg ttattaacga cagaagcagt cgttgcagat aaaccagaac cagcagcgcc
    32881 agcagctcct gcaatggatc catcaatgat gggcggtatg atgtagtaat taagaaagaa
    32941 ccctatttta acagggttct ttcttctttt tttgttttat tttaagcaaa aggggcaaag
    33001 aaggggcaaa aatatcaaag gctatttagt ttatcaatta cttgttgttt ggctcttttt
    33061 gttacatggt tataaataga cagagttgtg tttgcatcag aatgccctac acgctccata
    33121 atggctttaa gaggtacgcc taattcagat aatagtgaaa cgtggctatg tctgaatata
    33181 tgtgaggata gacttttttc taattccaac tcttcctcta ctttatggag tatagcgtta
    33241 aatgagtgca gcgcaagcgg agtgccactt gtagatataa atatatattg atcgggatct
    33301 gtgggtctac ctgcaagaat attgtcagct atcacacttt caatcaattc ttttgcacga
    33361 ttgggtaatt gcacttcacg ttgcgaataa gtattttttg gagttgtttt tatagcatta
    33421 tccattttca cagatgtgta atctaaggtc ccattaatgg aaattttccc atcctcatag
    33481 tccttcattt gcaaagctag caattcccca tatctcaaac cagttaaata tagaaactca
    33541 gctattatgc cgtgtagttt tctgcgagga ttggagtata gctgtttcag tatttgatca
    33601 atttcttctt tctccagata ttttttattc atagaaagcc ttcttttttc ttcttccact
    33661 tttttaggat ggattttaac tgctagcgca gggtttcttt gaatgtattt tctatcgatt
    33721 gcatagttta gcataacaga tagagttgtt tttgtttgtt ttgtgtagtt cagtgagagg
    33781 tcaccaaacg tatacatatc ttcaattatc ttattaatga gtgtctcatc aatgtttcta
    33841 acgatcgtat catcgcttat gtgcttagaa acatgtttca tcatcattgg aaccttcaaa
    33901 tagctagtac gtttaacatg ctgcttataa tattcatacc attctttata cagctcacca
    33961 aaagtgatat ctgatttatt gtaatcttcg agtgcttctt taattttttt atctagaatt
    34021 ttctgagctt ttttccacgc ttgtggtgaa ttacttgtaa gtgttgtaga tttttttcgt
    34081 gttttttctg tataaggatc tacatatctt tcaataaact tgaattggcc atctttggtc
    34141 tgttcaaccc acacttttaa catctcctat catttgctat aataggcata acaaatagac
    34201 ctatataggt ttgttttcta aaagcacgct cttactctgg acggtggggc gtgtttttta
    34261 tttattatac agttgcttta aaaatagctg ctttttcaaa tgatactaaa ggagagaagt
    34321 ggatttctat ttctcccttt tggtttaacg tgaaatgagc agttacatcc atttgttttc
    34381 ctggagcaac agatcccatt gtattttcgt tggcatatgt ctctgatttt ttgtcatctg
    34441 gtccatatac ttccacatct gtacctacag ggatatctga atcgccatca ttttttacgg
    34501 tataagtaat ttttactact tgtgcaggtt ggttttcttc aaattgattt ctttcatcag
    34561 ttagttctac actatttagt gtatattcag catcaccaac ttttacagtg tcaccaatct
    34621 tatagaaggt atcgcttttt tcttctttag aagatgaagc agaggttgaa gatttagtta
    34681 ctttttcgcc accattatca cttgctttat tactatctga tccaccattt aaagcagaac
    34741 caataattat aattaaaatt actgctaata cccaaaacca tacttttttg taaaaaggtt
    34801 tactaacttt atacattttt ccgtcttgac ccataacttt ttttgccatt taaatattcc
    34861 tcacttcttg ttataatata tttgcgatct cagaaatgag gtatgagtcc gtgtgccagc
    34921 acgggctttt ttaatgtttt gctgaaatcg gtttttttaa taattcttga tgtattcata
    34981 catattgcct tgcgtaagaa tattttttct taaaatagca ttggcagaca gcataacaag
    35041 tgtatctgag cttattctga atagaataat actccataaa tttttcgaga ttaaactgag
    35101 attcatcagt cagttcgttc tcaatataga tatttaataa aattagaata gctattttat
    35161 ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt atcgtacaaa actgaatatt
    35221 caaaatgaga agcggtgaaa tgcgcaagtt catgagataa atgaaaggct tctgcagttt
    35281 cgccatatag attttcattc aaaaaaatga ttctaggctt cggataatag aagccaggct
    35341 ctttcatttc catgtaaact actttcaaat tgtattcgct caacatttct ttcaatttca
    35401 aatacataca acccatcact ccaactattc attttcctct aaagctttag caattgcaat
    35461 cgctttacgc attgtctcct tagatatttc ttttccatca aaagaaaaaa cagtatcgtt
    35521 ttctgataaa tccacatgtt taggggtttc tctttcttct ctacctagaa ggtagtctac
    35581 agagactcca aagtaatcag ctaaccgagc taactcaata gaattaggag tttgtctttt
    35641 ccaacttcct aaatatccat tcgaataccc gaaattcatt tcgagctgtc taatggataa
    35701 tcctttttct tttgctaact cctttattat ttcgtaagta ttcattgata aatcaacctt
    35761 tctgaatgct tacaaaaaaa gtttagataa ataatgtaaa aatagttgac ttatatacat
    35821 tattaatcta tactatgttt tgtaaacaag tttaacaact aaaaagacaa caaaaaatac
    35881 tattgattaa taaatgccaa ccgccaagaa agctttttaa atcaaagttt atatgtctta
    35941 tttaactatg catttagtat agattattaa tctaataaag tcaacgcagt tttttaaaaa
    36001 gttgttaaat ttgtttacga atataaaaga aaggagagaa gaatatggaa aaaacagtct
    36061 cggcaaaaat cgaagatttg aaattagaca tactgaaaca agcaaaagtg gcgatggaac
    36121 acgcggtaga taaagaagac tctgccatgg ttgcagccat agcagagatt ttagctcacg
    36181 tttagtcatc gtttttacgg tcttctggaa gctgaccata gataacagca taatgaatat
    36241 ctaaataagc ttgaacaatt tcttttggcg aaattgattc gcctttagta gatacttgtg
    36301 attcgtgatt tagccatgca acaactaaat ccgcagcgat ttcaaacggc gttacacttt
    36361 gtgtcagttg ccataaagaa tttcacaata tgtatggtcg tggtaataat accaaagagc
    36421 aatttgagga atttatggaa attaaaaaat acaaaaaatc atttattaaa ggaggctaaa
    36481 caatgaacaa ggaaatgagc caaacaatca aggtacaaaa aatgatagat gatctaacac
    36541 atggaattga tagccaagcc gataaaatca tcaaggaatt gcaaggacaa aaagttaagg
    36601 atgcaaaaat gcttctcaag actatcaact ttgaaatgaa tccaacaaaa agaaagctcg
    36661 ccgatgtgtt ggaagaaaag ttggcttccg ctatcaacga gcaagaatta ctatttgaaa
    36721 ccgatacttt taacagctga tgttttatag atgacagttt catccgaatc tatatcatcg
    36781 ccgagagaaa agaatgttgc ttttgacaaa aagccgataa gacctacaag cggtcttgat
    36841 gttgcaagag tagatacccc aatatcacct tgcaaggtat tcacgtaaat ttgagaagaa
    36901 tagaacattc catctttttc ttcgatattt gacgcatcat cgtatccaac gaccaaagta
    36961 tcggcaaata ttttgatttt ttctccatca agcatagtaa ttacaaattc tggtttcata
    37021 aattttcacc tccttaacaa ttatttcagc ctgtcacact gataaggaaa ttataccaaa
    37081 gaaaggaatg agaaatatga atacaccaca aatttttaat ttcgagcaaa acgaagttcg
    37141 aactatttta gtaaatgatg aaccatattt tgtaggaaaa gatgtagcag acgttctagg
    37201 atattcgaat ccgcaaaaag ctattcgaga tcatgtggac ttggaggata agacgcagaa
    37261 cgattcgttc accgtcaatg gaacagcagt tgttttgatt aacgagtcag gcctttacag
    37321 tttaatctta aaatcaaaac ttccctctgc caaaaaattt aaacgttggg taacgagtga
    37381 agtccttcca acaattagaa aaacaggtag ctattcaaac gtacctcaaa gttttgcaca
    37441 agcattgcgt ttagcagcgg atttagaaga aaagaaccaa ttactcgaac aacaaattgc
    37501 cgagtacgaa ccaaagatta gctacttaga tacgattctt tcatcgacag atacggtagc
    37561 tacttctcaa attgcagctg attacggaat gtcggcaatt gctctaaaca aattgctaaa
    37621 cgagttaggt gttcagcata aagttagcgg acaatggata ctttaccgaa aacatatgaa
    37681 ccaaggatac acaaaatcgc acacaagtga gataccgaaa gccgatggcg gcactaaagt
    37741 tgtaatgaat accaaatgga cacagaaagg gcgagtgttt atttacaact tattaatcgc
    37801 agagggctat taccctcaaa tggatttaga ggaaattggt tagaaaggag ttttagtatg
    37861 actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata tgtcgaaagt
    37921 tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa aagctactta
    37981 tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat agattcgatt
    38041 atcactatgt acgaattgta gagggaggta aagtcgttgg aagataaaat cattcttaca
    38101 aaatcagagc ttcaagaaat gttaaacatc gagtatggaa cgataatttt taggagggat
    38161 aataagtgaa aaaaccaaca ctttcagagc tgatagaagc tgctgagaag gcagtaaaac
    38221 cagacgactg gtaccgacaa agtttaatct tggagaagtt ccacggcatg tcaaaaacta
    38281 ctttagttga atactgcaag gaaatggaaa caattcctga atttgcagaa ggaatacttc
    38341 gtccaggtca ctcaaccaca tttattcatt accatacttt tatttggttt ttaaagtgga
    38401 aagacgcaaa caaatatcgt gtaaaaagtt tatcgccttc agatgttttg aaggaggcaa
    38461 gttaatgggc aaattcaaca gagcactagt attcagcgca ccgctaatca tctacgcttt
    38521 aggcctctgg ggaagcagac aagcgttgat agggacaatc gtttacatgg tttggatttt
    38581 tatggggctt gatgaagctg agcacagagc gaaaaaagcc agtcgggagg gactgactaa
    38641 aatatgattt ttttattttt gttcctttgg gctgctattg cgtacttcat ccagctcctt
    38701 gttgtcaaag ttgctcttat ttcttttagg aagaacaaaa gaaaatagcg ctataaatga
    38761 aagataagta gccgctacat taaactgttc ttttgaaaaa gttacagtca tcggaatgat
    38821 aattgatatt gaggaagtaa tcttaattaa aaatatagca atattttcaa catcgcgttg
    38881 gtttttattt aacattgtcc aacatgtttt agcaacaata aaaataatta cacatgatat
    38941 tagcacacag actgaaaaaa gttttagaaa tgcgatgtag tcagtgttat tgaactcctt
    39001 caaaatacta tttttattca atgtaataaa aggaattttt ttaattgaag acaggaaaga
    39061 taaggtaata aacagaatta tatatatggt taaagtcgca gtaagaatgt aattgagctt
    39121 tttcctcata gaatcacctc actttctaac gaaaattata ccaaaaagga gagaagaaat
    39181 aatgcaagaa ttagtaattt tgaaaaataa agaagctgta actacgagct tacaagtggc
    39241 agaaagtttc aaaaagaaac ataagcatgt actagaagca attgaatcaa taaaaagatc
    39301 ggtcgaaaat tcggccaatg ttgaagatgg gtccaatttt ggacagatgt ttgtggaagg
    39361 gaacgagcca gactcatacg gaagaagtcg gagagtttat ttcatgaata gagacggatt
    39421 ttccttgcta gctatgggat tcactggaag taaagcaata aatttcaaac taaaatttat
    39481 tgaagctttc aacgaaatgg aagatgttat tcggaagaat actgttcctc aaacaattga
    39541 agacatgatg atctatcaat tagaagaaat gaaagatgtt aaaaaagatg tttccatgct
    39601 taaagatact atgcgaatta gcggacaaca agagtttgaa attaagcaaa aaggaaatat
    39661 gaaagttatg gaagttctag ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa
    39721 agtattctca aaattttggt ctgaatttaa acgtaccttt tcaatcccaa gatatggcga
    39781 gttacctcgt aagagattcg atgatgctgt ttcatttatt gaaatgtggt taccagaaac
    39841 tgcgatccgc atggaaatcg atcaactgaa cagacaacaa agacttttcg gtgatgaaaa
    39901 tgaatagagc tgaagcgcta agaataggga cggtaattgc taatcgctgg tggagacaca
    39961 ataaaccaaa catcctaagc caacaacata tcgaaaagca aaaagcatgg caacaaataa
    40021 aagctctata ctttttgatg ttggtggatg acaccagcat cttttttatt ttccaaaaca
    40081 tatagaaaag acaccttgaa atcctttaga attaatttgg cgaaccaacc caaaaggagg
    40141 aatcaagatg tcttacactc accttatcaa agaaacatta gatattctag acttatcagt
    40201 tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa gggcaaatct gtcatattta
    40261 tcgtggaaac ttgatttata cagcccaaga atgcatccac tgtaaacacc agattgcttc
    40321 agatattgtg cgttggggta caacaaccgt ccggttgtta atgaacgatg tttctgaatg
    40381 ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc aaagcttgtc agcggacctt
    40441 tgtcgcagat acttctgttg cgaaaaaaca ctgtttcatc agtgagaaag tccgctggtc
    40501 tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag atcgccgctc agaaaaatct
    40561 atcggtctct tctgtttacc gtattatgaa gcgattttat cgtccgttga atccttttcg
    40621 gacaccgtta cctaaggtac tttgttttga tgaattcaaa tcagttcggg gtgtttcagg
    40681 tgctatgagt ttcatcatga tggatggaca aacacagcgc ttgttggaca ttgtcgagaa
    40741 ccgacaactg ccttttttaa aacgttattt tagtcatttt tcacgagaaa ttcgtgaagc
    40801 ggtcgaatgg atcgtgattg atatgtacgc tccttatgta tcgctcgtta aaaagctgtt
    40861 tccaaaagca cagctgatca ttgaccggtt tcacatcgtc caacatattg gtcgtacctt
    40921 tcgcaatcat cgaatcaaag aaacgaacca actattaaaa agcaaagaac aaaaacacta
    40981 tcaattaggt aagcaattaa aaagatactg gaaacttctt caaaaagatg aacgcaagct
    41041 tgattatact cgacgccttt ggcgacctgg cttcaaagct catttaacag agaccgatat
    41101 tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta ggctatcagt tatatcaaga
    41161 ctttctttac gctgtaaagg aacgagacta cgtatcgttt gaagaactac ttaccaataa
    41221 tatcatgctt cccgaaggct atcagacgac cttgagaacg ttccagaaat ttctgccaca
    41281 aatcaaaaat gctttacaac aaagttattc aaatggccca cttgaatgtc tgaacaacca
    41341 tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc ttttataatt tcaaattgcg
    41401 catcatgatt cgtcatggca acgcattgat tttcaactaa aaaagatcaa gaggaactga
    41461 tccacttgat ctc
//
LOCUS       DACECJ010000017        39175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.017, whole genome shotgun sequence.
ACCESSION   DACECJ010000017 DACECJ010000000
VERSION     DACECJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.017"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..560
                     /locus_tag="IWO09_000939"
     CDS             <1..560
                     /locus_tag="IWO09_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0263550.1"
                     /translation="KNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKH
                     IFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAH
                     KVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEV
                     GYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            760..1074
                     /locus_tag="IWO09_000940"
     CDS             760..1074
                     /locus_tag="IWO09_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0263551.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1095..1472
                     /locus_tag="IWO09_000941"
     CDS             1095..1472
                     /locus_tag="IWO09_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0263552.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1482..2255
                     /locus_tag="IWO09_000942"
     CDS             1482..2255
                     /locus_tag="IWO09_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263553.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2277..3680
                     /locus_tag="IWO09_000943"
     CDS             2277..3680
                     /locus_tag="IWO09_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0263554.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3862..5046
                     /locus_tag="IWO09_000944"
     CDS             3862..5046
                     /locus_tag="IWO09_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0263555.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5043..5333
                     /locus_tag="IWO09_000945"
     CDS             5043..5333
                     /locus_tag="IWO09_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263556.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5330..5551
                     /locus_tag="IWO09_000946"
     CDS             5330..5551
                     /locus_tag="IWO09_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263557.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5593..6372
                     /locus_tag="IWO09_000947"
     CDS             5593..6372
                     /locus_tag="IWO09_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ0263558.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6439..6933
                     /locus_tag="IWO09_000948"
     CDS             6439..6933
                     /locus_tag="IWO09_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0263559.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6989..7627
                     /locus_tag="IWO09_000949"
     CDS             6989..7627
                     /locus_tag="IWO09_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263560.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7699..8094
                     /locus_tag="IWO09_000950"
     CDS             7699..8094
                     /locus_tag="IWO09_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0263561.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8078..10531
                     /locus_tag="IWO09_000951"
     CDS             8078..10531
                     /locus_tag="IWO09_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0263562.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10528..12555
                     /locus_tag="IWO09_000952"
     CDS             10528..12555
                     /locus_tag="IWO09_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263563.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12552..13574
                     /locus_tag="IWO09_000953"
     CDS             12552..13574
                     /locus_tag="IWO09_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0263564.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13591..14520
                     /locus_tag="IWO09_000954"
     CDS             13591..14520
                     /locus_tag="IWO09_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0263565.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14765..14881
                     /locus_tag="IWO09_000955"
     CDS             14765..14881
                     /locus_tag="IWO09_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0263566.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14897..15700
                     /locus_tag="IWO09_000956"
     CDS             14897..15700
                     /locus_tag="IWO09_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0263567.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15875..16171
                     /locus_tag="IWO09_000957"
     CDS             15875..16171
                     /locus_tag="IWO09_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263568.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16178..18130
                     /locus_tag="IWO09_000958"
     CDS             16178..18130
                     /locus_tag="IWO09_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0263569.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18202..18699)
                     /gene="dfrG"
                     /locus_tag="IWO09_000959"
     CDS             complement(18202..18699)
                     /gene="dfrG"
                     /locus_tag="IWO09_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0263570.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19061..20101
                     /locus_tag="IWO09_000960"
     CDS             19061..20101
                     /locus_tag="IWO09_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0263571.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20199..20387
                     /locus_tag="IWO09_000961"
     CDS             20199..20387
                     /locus_tag="IWO09_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0263572.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20445..20798)
                     /locus_tag="IWO09_000962"
     CDS             complement(20445..20798)
                     /locus_tag="IWO09_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0263573.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21329..21751
                     /locus_tag="IWO09_000963"
     CDS             21329..21751
                     /locus_tag="IWO09_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0263574.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21748..21978
                     /locus_tag="IWO09_000964"
     CDS             21748..21978
                     /locus_tag="IWO09_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0263575.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22476..22676
                     /locus_tag="IWO09_000965"
     CDS             22476..22676
                     /locus_tag="IWO09_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0263576.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22703..23896
                     /locus_tag="IWO09_000966"
     CDS             22703..23896
                     /locus_tag="IWO09_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0263577.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23964..25529)
                     /gene="guaA"
                     /locus_tag="IWO09_000967"
     CDS             complement(23964..25529)
                     /gene="guaA"
                     /locus_tag="IWO09_000967"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0263578.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25588..26511)
                     /locus_tag="IWO09_000968"
     CDS             complement(25588..26511)
                     /locus_tag="IWO09_000968"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0263579.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26707..27312
                     /locus_tag="IWO09_000969"
     CDS             26707..27312
                     /locus_tag="IWO09_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0263580.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27620..28636
                     /locus_tag="IWO09_000970"
     CDS             27620..28636
                     /locus_tag="IWO09_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0263581.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28777..30078
                     /locus_tag="IWO09_000971"
     CDS             28777..30078
                     /locus_tag="IWO09_000971"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0263582.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30124..30786
                     /gene="deoC"
                     /locus_tag="IWO09_000972"
     CDS             30124..30786
                     /gene="deoC"
                     /locus_tag="IWO09_000972"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0263583.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30807..31205
                     /locus_tag="IWO09_000973"
     CDS             30807..31205
                     /locus_tag="IWO09_000973"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0263584.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31271..32353
                     /locus_tag="IWO09_000974"
     CDS             31271..32353
                     /locus_tag="IWO09_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0263585.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32565..34127
                     /locus_tag="IWO09_000975"
     CDS             32565..34127
                     /locus_tag="IWO09_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263586.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34120..35253
                     /locus_tag="IWO09_000976"
     CDS             34120..35253
                     /locus_tag="IWO09_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263587.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35253..36209
                     /locus_tag="IWO09_000977"
     CDS             35253..36209
                     /locus_tag="IWO09_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263588.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36308..37474
                     /gene="deoB"
                     /locus_tag="IWO09_000978"
     CDS             36308..37474
                     /gene="deoB"
                     /locus_tag="IWO09_000978"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0263589.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37498..38316
                     /locus_tag="IWO09_000979"
     CDS             37498..38316
                     /locus_tag="IWO09_000979"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0263590.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38333..39040
                     /gene="deoD"
                     /locus_tag="IWO09_000980"
     CDS             38333..39040
                     /gene="deoD"
                     /locus_tag="IWO09_000980"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0263591.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        12978 a         6763 c         8013 g        11421 t
ORIGIN      
        1 taaaaaatga atttggaata gaagttaaag attattttat tgacttagat ttattacagc
       61 taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat ttatacaaat
      121 ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac ccgtctattt
      181 ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat aattcattat
      241 ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc acacttacta
      301 cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat cttctaaacg
      361 ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt agttcagcga
      421 gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt ttctcagagg
      481 tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta gataaaatag
      541 aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata gtaggaatcg
      601 gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg aacttatgaa
      661 aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca cacaaataag
      721 attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa atatgtcatt
      781 cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa cattgaacaa
      841 cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata ctcaacgatt
      901 caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa acattttgaa
      961 tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta taaaattggc
     1021 aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc ataagaaagg
     1081 aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac cagacatttg
     1141 gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa gatggtacgg
     1201 tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa ggacgcatga
     1261 tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac gcagaagtgg
     1321 aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg gcagacgttg
     1381 attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct catcagaaat
     1441 ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac acaccattga
     1501 ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac cattattcaa
     1561 agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa aaattattag
     1621 gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa ctctttcccg
     1681 acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt cttccagaaa
     1741 tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag tggttgaaag
     1801 aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga aaatgtggct
     1861 atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt gtctatcgtg
     1921 ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta gaggaaaatg
     1981 ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc gaatacaaag
     2041 ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca aaagaagcga
     2101 aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa aaaaatttac
     2161 gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca acttttgata
     2221 aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg atttttatgc
     2281 cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg gtctatcatt
     2341 ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt cattggagag
     2401 cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat caacttcata
     2461 cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt atcagctggc
     2521 tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa aagttagctc
     2581 gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag ggattcttta
     2641 aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa atatattatc
     2701 gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa tatcaagatc
     2761 agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta gtggaaaaaa
     2821 cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc aatcgaattg
     2881 gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg gtctattggg
     2941 agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga aaatcctatt
     3001 ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat attcttgacc
     3061 ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat taccgtaaag
     3121 aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc agtgaaacca
     3181 tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt ttgtctgcca
     3241 attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt gaaagtacca
     3301 ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc ttctttttaa
     3361 ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg gacaacttta
     3421 acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg ttcggaagcg
     3481 atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg gatacaggaa
     3541 aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat ttcttaggag
     3601 aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa gtgaaaggta
     3661 caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt agccccctct
     3721 atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac caatgttgat
     3781 ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag tttacatctt
     3841 ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga attgaaagaa
     3901 aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca tattaccaga
     3961 ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt gaaagaagat
     4021 ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt attcgactat
     4081 gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt tttacgtttg
     4141 aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga acactatcgt
     4201 ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt attagaactg
     4261 aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg gagctggtat
     4321 gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga tttagctatc
     4381 aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa acaggaagaa
     4441 tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt tcaccgtgat
     4501 gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga agtctatttc
     4561 tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt agaagacgca
     4621 gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca agccatgaaa
     4681 gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa tcgctatatc
     4741 cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa tgaacgttgg
     4801 gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc agagccttat
     4861 tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt aaaaatcgca
     4921 agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca ggaagccaaa
     4981 ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga ggatttgatt
     5041 acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc attgagtttc
     5101 aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga aaacggcaag
     5161 ctggtgatac catctatacc tatgattttt acaaggtgag attttataat aattatacca
     5221 atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg cgtgtaaaaa
     5281 gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca tgaattttgg
     5341 gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt tagcgattgt
     5401 tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg gcttcttgat
     5461 tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag atgtgttatt
     5521 aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa aataggaagt
     5581 atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt taaatctctt
     5641 ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg taacgagatt
     5701 gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt tacttattat
     5761 aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag agttcctttt
     5821 gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc aagctccata
     5881 tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact tccagagaat
     5941 tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca agctattgaa
     6001 gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag agttcacact
     6061 gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt tcctagagac
     6121 gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga aattcaacct
     6181 actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat gattagctct
     6241 cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat tattggaaag
     6301 agagaagcaa aactaataat aggtatttca agtacagtta cagattatat tagcggatac
     6361 cttgataagt agcgtcatta actgattgct acttttttta tgactatttt tataaaaagg
     6421 atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta taatgaaggt
     6481 gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc agaaagaatc
     6541 ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc ttttgatgtg
     6601 gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca agaaatcgaa
     6661 ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag tagcttggaa
     6721 gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc gatggaagat
     6781 gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc caatttacaa
     6841 aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa ttacctagtt
     6901 acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt ataattaaat
     6961 taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc ttaaactatt
     7021 aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc gttacaaaca
     7081 gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc
     7141 tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag ctgtctatgc
     7201 taattcggta aaattatata cagacacgaa aacaataaga gaatattttg gtatcgaata
     7261 cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc gatttattcc
     7321 gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag
     7381 tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa gaaatccttt
     7441 aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa cgagattgta
     7501 tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca gatattcaat
     7561 gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa aaaataaaat
     7621 tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt tctattttta
     7681 cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg gtcagtagaa
     7741 aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt tacgcaaatg
     7801 acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc accactttct
     7861 ctgattgacg gagcactact caaatactta gggattccca ttgttctcac tgggttcatg
     7921 tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat cattacctat
     7981 gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa agaaacaatg
     8041 aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga ttaaatatat
     8101 ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg aattaattcc
     8161 ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata atttctgtca
     8221 attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa ccgaatcaag
     8281 tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa aagagatagc
     8341 ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg acaatcaagt
     8401 agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa
     8461 aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa
     8521 attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga
     8581 acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga
     8641 ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga
     8701 ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct
     8761 caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga
     8821 taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc
     8881 taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat
     8941 gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga
     9001 actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt
     9061 ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat
     9121 gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg
     9181 ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg
     9241 cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta
     9301 tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat
     9361 tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct
     9421 ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc
     9481 ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc
     9541 agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa
     9601 agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga
     9661 caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct
     9721 tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc
     9781 tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt
     9841 gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga
     9901 aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc
     9961 cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc
    10021 caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt
    10081 cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga
    10141 ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt
    10201 ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga
    10261 tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga
    10321 cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa
    10381 tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg
    10441 tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag
    10501 accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat
    10561 agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat
    10621 gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga atactccaag
    10681 tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca
    10741 tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat
    10801 tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc
    10861 tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt
    10921 gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt
    10981 tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg
    11041 aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt
    11101 gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc
    11161 agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt
    11221 gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt
    11281 caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct
    11341 accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa
    11401 gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc
    11461 tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg
    11521 attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta
    11581 agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg
    11641 attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg
    11701 ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc
    11761 tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac
    11821 gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa
    11881 accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag
    11941 caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat
    12001 gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt
    12061 atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc
    12121 aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc
    12181 cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca
    12241 gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac
    12301 attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca
    12361 tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct
    12421 gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa
    12481 caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga
    12541 ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct
    12601 tttttctctt attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca
    12661 acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc
    12721 gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct
    12781 tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat
    12841 ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat
    12901 atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc
    12961 aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata
    13021 cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc
    13081 ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt
    13141 atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt gatgatgaaa
    13201 ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag
    13261 gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag
    13321 ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta
    13381 tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat
    13441 atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa
    13501 tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg
    13561 ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag
    13621 aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa
    13681 gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga
    13741 tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc
    13801 attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa
    13861 gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc
    13921 cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct
    13981 actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc
    14041 tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata
    14101 tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa
    14161 aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt
    14221 gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc
    14281 cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta
    14341 cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa
    14401 gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc
    14461 caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat tgtaaaataa
    14521 caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt
    14581 ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag
    14641 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc gggaacaaat
    14701 aattagatgt ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta
    14761 tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt tgtataccta tggttatgca
    14821 taaaaatccc agtgataaaa gtatttatca ctgggatttt tatgcccttt tgggtttttg
    14881 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcagg
    14941 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
    15001 cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac
    15061 aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac
    15121 gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc
    15181 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc
    15241 acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
    15301 tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa
    15361 acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg
    15421 ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt
    15481 agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgtt ctctgttccc
    15541 tctttatcat ggaagtgcaa aaagtaatat agggattgat aaccttatag aagttattac
    15601 taataaattt tattcatcaa cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa
    15661 aattgaatat acaaaaaaaa gacaacgtct tgcatattaa gtagttcagt tttggagtac
    15721 aaaaagtgaa aatgtaaacc taaatgagta tcaaattgca actgaatttg atactcgttt
    15781 tttattgtta aatcaacgtt tgttgttaat ttacaccaca aaatgtaagt taaaatgtat
    15841 ataaaaatgc gaacgtgaaa taattattgt aggaatgcaa attggtgtgt taaaatggtt
    15901 acaatttact aagcaaaaag gggtgcattt tcctatgcaa tttcttgagc caaaaaataa
    15961 gaacgctaag tctgtggatt gggaaatatc agagcaagta agggtgatag ttaagcaata
    16021 cgctgaatat gcagagcgta ctgaaagcga agcagtggat gaattcttat tgaacatttt
    16081 agacgataag aagtttattg aatggattgc caacaagcga agtaataagc ggattgttga
    16141 aaaaatgggt atcaaagata gagtgggttg atagacaatg accaagctta aaagattagc
    16201 cacgaaggaa gatgaaattg tagatgttaa aattccgatt tcaaacgaag aattaaaaga
    16261 tagggctaaa caatatgatt tattaactcc aaaacgattt gcaacaaggt ataacaaaat
    16321 gttgttcttg ccaacgtctt ttaaatggaa cggttctgaa tatcccattc aatataatta
    16381 ctgcatcaat ccgttttgtt gtaattttgg gaaagaacag cacaaattca aagatgtaaa
    16441 agggaaacca agtcgatata aaatgactgg tagttccaaa gataaaggtc ataaaggtat
    16501 gtactgcaat gacaatccta ttggcagagg tgtatcgcaa aactgtacag taaccccttt
    16561 atctaattgg tctgttgttg aagaaattaa acgccttata gaaatcaata gcatccaaga
    16621 tgttgagcca gattatcaat ttcataaaga aggctgctcg gaagaagaat caaccccatt
    16681 caatgagccg aagcaatttt acaaaagggg gaaaagtagg ggcaaatctc aacgctatca
    16741 atgtaaggcg tgtaagaagt tcactaatgt tctaccaaaa agagaagaaa ccactactta
    16801 tcaccaacaa aagaacacca ttcttccaat gttcgctaag atggttgttg gaagggtttc
    16861 agtcagtcgt acttgtgata ttttaggaat tggagttgga acctactatc ataagctgga
    16921 gtggctgtat aggcgttgtt tggagttttt agaacgatat gagacacaac cccttcaaac
    16981 aaagaaattt aacgagatgt ggcttaatac ggacaagatg cactactacc taaataacgt
    17041 ccgtaagaaa ggacaaggtt caaagaaata tacggggttt gaagacctta atatgcaaac
    17101 gtatatagtt gtatctgctg aggtcttatc aagatatgtg ttccgttctg atgttgccta
    17161 tgattggaat atttcaatgg atgaattaaa tgaagataca agaaaattca aagaagacca
    17221 tctcaatact tttagtcgta aaaatgatag gttagattgg tcttattatc cacaagaacc
    17281 atccgctaac gatagcgaaa ataggaatgc ttatcttcac gaattaggga aaattactaa
    17341 taggagtaga tttgtcgatg gtttaaatgt tgatgctcct tatactacaa ctgctcacta
    17401 ttggttaatt aaacaaatgg ttaatgccga cgaatggcga atgattagtg atgatgactt
    17461 ctctattaga aacgcctttt atagagtatt cacgaaagaa ttaaggttga gcgatgcaca
    17521 tcattttatt tgccaagtta acaagacgaa aagcagaaaa caatgcttaa aagaattcgg
    17581 acaagcaaaa gcagaactgc tcgattgggg agatatcaga ggattcaaaa caaagtttct
    17641 acggactttg gcatctcatt atctaactga attactaaca agtcatcaat tccacgaaga
    17701 ggctattagt aaagatggag aaagatatcg caaatatgcc gataacccta tcaaacatcc
    17761 attagccact aaggataaag gattttattc agttgattgt agaacggatt tatctgctct
    17821 ggaaccaaac gaaatcgcaa agatgctatt aaatgttaat gaccattcga ccaatagctt
    17881 tattcaacag ataagacgtt atatatcatc tcttgaaaga ccattaacaa cagctcgtgg
    17941 tgataagaag agctatattt acgctaactt caatccgaag tatgctcaat ttgcgattac
    18001 aattctaaga acttactaca acttttgtcg cccttttaaa tcagcggata aaaaggtttt
    18061 gacacctgct caaagattag ggattacaga taaacaattt gattggaaag atataattta
    18121 tttcaaatga caaaaagagc ttggagtaat cccaagctct tgtgctttat ctttactgtt
    18181 cttcttcaat taacgcaccc gttaactcaa taagtttttt ctttcatata catgaaaata
    18241 gtagttatac ggatttttat cattctttat ccctttttgg gcaaatacct cattccattc
    18301 ctcataattc acttctggaa aaaaagtatc tccttcgaat tcatgatgta tttttgtgat
    18361 gtacattttc tcaacataag ggaaaaacaa attataaatc tgttctcctc cgaaaataaa
    18421 aatttcttct tcgtttttac ataactcaaa aacatcttct attgaatgaa caatttcaca
    18481 accattaaag gtaaacccct tatctctcgt cagaataata tttcttctgt caggtaaggc
    18541 tcttccgatt gattcaaggt tcttcctacc taatattatc ggatgtccct ttgtagtatt
    18601 tttaacatat tcccagtcct tgggaatcct ccaaggaatg tcattctctt tgccaatcac
    18661 tctattctta tccatcgcag caatcaaaga aactttcatt tataacacag cctttttaac
    18721 taatttacca tctaatttct tatttctatc aaaaaacctt ttctcattat cctgcccgtt
    18781 agttgaataa gcaatttttt ctataagtta tattatcata ataccacaaa atcacaaatt
    18841 ttcattcaat tatcattttc aattgcattt tataaatcat tttgtatgta atttggtatg
    18901 taagttaaaa tgaaaaaagt agtccatttt gaagtacatt ttgtcatttt gtactccaaa
    18961 actgaactac ttacttgcat atatacgcct ttatagtgga gtactacatt tacgagattc
    19021 ggttagagta tcagaaaaag aaaaaataaa agttacagaa atgtatactt caataaatgg
    19081 tgaattatgt aagattgata gagcttattc tggagaaatt gttattttgc aaaatgagtt
    19141 tttgaagtta aatagtgttc ttggagatac aaaactattg ccacagagaa aaaagattga
    19201 aaatccgcac cctctactac aaacaactgt tgaaccgagt aaacctgaac agagagaaat
    19261 gttgcttgat gcccttttgg aaatctcaga tagtgatccg cttctacgat attacgtgga
    19321 ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg aagtgattag
    19381 tgcactgttg caagaaaagt atcatgtgga gatagaacta aaagagccta cagtcattta
    19441 tatggagaga ccgttaaaaa atgcagaata taccattcac atcgaagtgc cgccaaatcc
    19501 tttctgggct tccattggtt tatctgtatc accgcttccg ttgggaagtg gaatgcagta
    19561 tgagagctcg gtttctcttg gatacttaaa tcaatcattt caaaatgcag ttatggaagg
    19621 gatacgctat ggttgcgaac aaggattata tggttggaat gtgacggatt gtaaaatctg
    19681 ttttaagtac ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac
    19741 tcctattgta ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct
    19801 tagttttaaa gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa
    19861 atattgtgca aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga
    19921 aattcctgct cgatgtattc aagattatcg caatgattta acttttttta caaatgggct
    19981 tagtgtttgt ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca
    20041 gacccgtcgt ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta
    20101 gtgcgtttta tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta
    20161 atatagattt gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat
    20221 tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa
    20281 atttcccttt atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga
    20341 atataataac aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa
    20401 actctcatct tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt
    20461 cttgcttcat tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag
    20521 ctatccatgt atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt
    20581 aagaaaaatt catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta
    20641 cttgggtgtt gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt
    20701 gttcccactt gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca
    20761 acggctctaa aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt
    20821 tactgttcct atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc
    20881 ctatagaaca ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct
    20941 gattttattt acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa
    21001 ttggcgactt aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct
    21061 taattaagtt cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg
    21121 tggttttgca gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt
    21181 ttatgtgctt ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac
    21241 aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc
    21301 ctttcacgac agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga
    21361 aaatcagttt gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa
    21421 acatctagac agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt
    21481 caactctttt tctactgttg atacgtacgt cactgatttc caactattta aattatttgg
    21541 attaagaatc aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa
    21601 aaaacggaac attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga
    21661 gttactaaat ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat
    21721 taaagaattc atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc
    21781 ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat
    21841 cgagggtata cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat
    21901 atggttattg atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta
    21961 tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt
    22021 cccgatttgg gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata
    22081 ggctaacaag ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa
    22141 gaaaagagct gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag
    22201 taattcgaaa gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata
    22261 ctcccgtata gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg
    22321 agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata
    22381 ttcttctctt gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg
    22441 agttttccta gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt
    22501 tagttgagct aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat
    22561 tgatgattac aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg
    22621 aagctgttga agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt
    22681 tgcacagtaa ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac
    22741 ctattatttt cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg
    22801 tagtggattc actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa
    22861 accagaagaa ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat
    22921 tttccttcca tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac
    22981 tattaagaag catttctctt attttgataa aatggctgtt tccgatattg agcctattga
    23041 tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt
    23101 tcaaggacta ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa
    23161 tccatctaag attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa
    23221 agaagaattt gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt
    23281 atttatctct ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat
    23341 tcaatgggaa gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta
    23401 caagaatcaa tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat
    23461 tgtattagat aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct
    23521 aattcagaca gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc
    23581 atatgctatt gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt
    23641 acggcattct catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga
    23701 tcgtctagga cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa
    23761 cagtaatttc gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca
    23821 aaattatgat acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat
    23881 gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa
    23941 gaggatttct agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg
    24001 atgtcataca cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt
    24061 tgcaagactt cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca
    24121 gcacggatac cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg
    24181 atccctggaa gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt
    24241 tcttcacgca agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg
    24301 ccaagaacac gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca
    24361 ggcatgccta actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt
    24421 tcgatcaatt caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc
    24481 gtctgtgctg tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg
    24541 aaagagacgc cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat
    24601 tcattcccaa tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag
    24661 aagcgatctc ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc
    24721 attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat
    24781 tggtcaccga tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa
    24841 agaccaagca agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg
    24901 ataaagttat ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc
    24961 agcaattcga ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa
    25021 ttacgtgttg tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca
    25081 aagccttcag gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt
    25141 ggtgtttctt taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat
    25201 tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca
    25261 tagcaaatcc ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca
    25321 ttctcatcat atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct
    25381 tttacttctt ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga
    25441 atacgtcttg tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca
    25501 acgttcgtca tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc
    25561 tcaaaatagt caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat
    25621 atgattttca gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc
    25681 ctgtaaattt ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc
    25741 acgatctccg atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag
    25801 taacgcacca aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata
    25861 gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt
    25921 attgatcccc tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat
    25981 gatatcatac acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac
    26041 acggttcaag aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc
    26101 catgattcca cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag
    26161 ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt
    26221 actttttcca acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta
    26281 tttatgaaga aataaacctt tactcaagaa caaagattca aattctttca tataaagata
    26341 aatcagatga gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt
    26401 caaacttttg atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa
    26461 ttctcgccat tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc
    26521 ctgcgttctc ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta
    26581 agagagattg ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct
    26641 tgacttgcgc acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg
    26701 gaagacatga caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa
    26761 tgggcttttg agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca
    26821 cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta
    26881 cctgatgaag gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg
    26941 gcatttgcga cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta
    27001 gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc
    27061 tatgaagcag ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct
    27121 ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt
    27181 acactgacaa ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa
    27241 gaaacatttg gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt
    27301 gtgaaagaat aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat
    27361 gtacgggtac gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt
    27421 ttcttcaaaa agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat
    27481 acagaacttg tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca
    27541 tactttcctt ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag
    27601 atagaaggga gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt
    27661 atcagtaaca actgtctcac agattttgaa taacaaagga agcagattta gtgataagac
    27721 aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc
    27781 aaatgtgatc aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt
    27841 cttcttctcc aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat
    27901 catgttatgc aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc
    27961 ccaccgctca gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc
    28021 attagacagc ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg
    28081 tcgggataac ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct
    28141 gtttattgaa aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca
    28201 attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa
    28261 tgggaaattt gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa
    28321 attactcaaa cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg
    28381 atgctatcag gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg
    28441 attcgatgga ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc
    28501 aagttttgat attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca
    28561 aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt
    28621 attgacaaag ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta
    28681 gtgctttttt tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa
    28741 tgtataatac cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa
    28801 aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat
    28861 acgaatggaa agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa
    28921 gacatgacaa atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc
    28981 atcgatttga gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt
    29041 gacacgacca cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa
    29101 atgtctggac gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc
    29161 ggatttcaga tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta
    29221 gcggtaattg gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt
    29281 gacgtcacag cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa
    29341 atcgcggcag gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg
    29401 aagaacatcg aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct
    29461 aaccgagaaa cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga
    29521 aatagtctag aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta
    29581 gtagaaatgt gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca
    29641 accgatgaag caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa
    29701 ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga
    29761 attttgactg cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa
    29821 atcgtggcaa atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa
    29881 gaagaagata ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg
    29941 acaaaaggtg agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt
    30001 gaattattat ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat
    30061 gacatcatca cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa
    30121 gaaatggaac taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa
    30181 gctgtgatgc aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat
    30241 ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca
    30301 gtaatcgggt ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat
    30361 gccatcaata acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca
    30421 aaggatgatc aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt
    30481 gcgcttgtca aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct
    30541 tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca
    30601 ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt
    30661 gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt
    30721 gctacaagaa tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc
    30781 tattaatttt ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag
    30841 ctattgaagc gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc
    30901 tcgtaacgga atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat
    30961 taacgaattg tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat
    31021 ttacgcatct agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc
    31081 gacaagtaat ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg
    31141 gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc
    31201 tataactcat gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg
    31261 ctttacagaa atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg
    31321 gttattttta ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc
    31381 atcagacaca acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt
    31441 caaccagtca gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg
    31501 aaatgatggc ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga
    31561 ccaagcattg aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc
    31621 gatccaagaa caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat
    31681 cgatggatta gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc
    31741 aggtgttgca gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa
    31801 aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa
    31861 agaattagga aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga
    31921 taaaggacgt tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc
    31981 ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga
    32041 caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt
    32101 aaacggcgaa aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt
    32161 agaagatttg gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta
    32221 tggattgaaa gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa
    32281 agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc
    32341 tgaagaaaac taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt
    32401 ttgattagaa caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga
    32461 acaactccct gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag
    32521 tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata
    32581 ttgtcataga aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca
    32641 tcaatttaca agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca
    32701 aatctacatt gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga
    32761 tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga
    32821 tggtccatca gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg
    32881 gaagcgagcc tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa
    32941 aagtttctga acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag
    33001 ttggaatgga acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga
    33061 tttttgatga gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca
    33121 tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa
    33181 tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca
    33241 acgttaaaga cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat
    33301 tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag
    33361 tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg
    33421 gcgaagtact tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt
    33481 tgactggttt gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta
    33541 ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata
    33601 aatacgggtt agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc
    33661 aaaaacctta cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag
    33721 aattgatcga agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat
    33781 caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt
    33841 tgattgttgc aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac
    33901 gtctgatcga acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag
    33961 aaattttaaa tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg
    34021 ttgatccgaa agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag
    34081 aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa
    34141 ttccgtaatt tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata
    34201 ttgatggctg cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc
    34261 cttggttcgc aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca
    34321 gcattaggtt tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa
    34381 gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa
    34441 gttgttttgc ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct
    34501 gttccaggtt tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg
    34561 aactatatct tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat
    34621 cgctcaagtt tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg
    34681 ttaacacaag cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg
    34741 cttattttcg tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt
    34801 ggattgaatc cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg
    34861 tcgatggtca tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg
    34921 tatcaaaact tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt
    34981 tccttgcttg gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt
    35041 ttgaagatcg gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat
    35101 gtatcgattg ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg
    35161 gctaaagtct tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca
    35221 acgaatcaaa accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga
    35281 tccttacttc tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt
    35341 tttctgaacg aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt
    35401 ttagttccat cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga
    35461 tcgctatttt agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa
    35521 cagtcagttt gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg
    35581 ctttaggtgt tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc
    35641 agaatctggg gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt
    35701 tctttaaaaa tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg
    35761 tgatcatctt caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag
    35821 ctgccgacac attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg
    35881 gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag
    35941 ctggtacgat cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga
    36001 atcctttagg ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca
    36061 ttggcgcaca attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat
    36121 atttgctgac aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa
    36181 acgggaagaa ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg
    36241 gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg
    36301 aataatcatg tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc
    36361 accagatgcc gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga
    36421 agcaggattg aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga
    36481 aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt
    36541 agggaaagat acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt
    36601 tcgtgtgttt cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg
    36661 ccgtaaagtc gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga
    36721 acaccaaatg aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat
    36781 cgcggcacat gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg
    36841 tgatataaca aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg
    36901 tgaaccagga gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg
    36961 acacacagtc cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat
    37021 caacgatatt ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga
    37081 tggcgttgac cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa
    37141 tttagtagat tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc
    37201 gattgaagaa tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt
    37261 gctaatgatt acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg
    37321 agaatatgta ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca
    37381 aggatattat tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga
    37441 aaatggcgaa agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg
    37501 accaagttaa gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa
    37561 gaaattgatt ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa
    37621 gatgctgtaa tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg
    37681 catgcaggaa agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg
    37741 agattccact actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg
    37801 gccggactag gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca
    37861 tttgtaccag gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct
    37921 ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat
    37981 acgcctgact atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa
    38041 gaaggcgtat atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg
    38101 tctcggacaa taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca
    38161 gctcacagcg gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg
    38221 caagcgaatc tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt
    38281 aaagcattgg ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt
    38341 gcatattgaa gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt
    38401 gcgagctaaa tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg
    38461 cggtatgtta ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg
    38521 aatgggaatg ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa
    38581 gaaattgatt cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt
    38641 agtactagca caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt
    38701 tcattttcct ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga
    38761 aaagggctat acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga
    38821 tttgacagag acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc
    38881 ggctctttat tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag
    38941 cgaccatttg atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga
    39001 aatgatcgaa gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc
    39061 cctgcattaa gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct
    39121 ggacttatta ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaa
//
LOCUS       DACECJ010000018        37357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.018, whole genome shotgun sequence.
ACCESSION   DACECJ010000018 DACECJ010000000
VERSION     DACECJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.018"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IWO09_000981"
                     /pseudo
     CDS             75..470
                     /locus_tag="IWO09_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IWO09_000982"
     CDS             790..1104
                     /locus_tag="IWO09_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0263592.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IWO09_000983"
     CDS             1208..2386
                     /locus_tag="IWO09_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0263593.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="IWO09_000984"
     CDS             2789..3082
                     /locus_tag="IWO09_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0263594.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            3925..6252
                     /locus_tag="IWO09_000985"
     CDS             3925..6252
                     /locus_tag="IWO09_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0263595.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            6378..6707
                     /locus_tag="IWO09_000986"
     CDS             6378..6707
                     /locus_tag="IWO09_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0263596.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            6747..7751
                     /locus_tag="IWO09_000987"
     CDS             6747..7751
                     /locus_tag="IWO09_000987"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0263597.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            7748..8194
                     /locus_tag="IWO09_000988"
     CDS             7748..8194
                     /locus_tag="IWO09_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0263598.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            8344..9159
                     /locus_tag="IWO09_000989"
     CDS             8344..9159
                     /locus_tag="IWO09_000989"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0263599.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            9365..10537
                     /locus_tag="IWO09_000990"
     CDS             9365..10537
                     /locus_tag="IWO09_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0263600.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10699..11193)
                     /locus_tag="IWO09_000991"
     CDS             complement(10699..11193)
                     /locus_tag="IWO09_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263601.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            11776..12198
                     /locus_tag="IWO09_000992"
     CDS             11776..12198
                     /locus_tag="IWO09_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0263602.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(12364..13557)
                     /locus_tag="IWO09_000993"
     CDS             complement(12364..13557)
                     /locus_tag="IWO09_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0263603.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            13781..14158
                     /locus_tag="IWO09_000994"
     CDS             13781..14158
                     /locus_tag="IWO09_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0263604.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            14146..14484
                     /locus_tag="IWO09_000995"
                     /pseudo
     CDS             14146..14484
                     /locus_tag="IWO09_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14618..15061
                     /locus_tag="IWO09_000996"
     CDS             14618..15061
                     /locus_tag="IWO09_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0263605.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            15322..16137
                     /locus_tag="IWO09_000997"
     CDS             15322..16137
                     /locus_tag="IWO09_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0263606.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            16147..16809
                     /locus_tag="IWO09_000998"
     CDS             16147..16809
                     /locus_tag="IWO09_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0263607.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            17017..18681
                     /gene="alsS"
                     /locus_tag="IWO09_000999"
     CDS             17017..18681
                     /gene="alsS"
                     /locus_tag="IWO09_000999"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0263608.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            18694..19404
                     /gene="budA"
                     /locus_tag="IWO09_001000"
     CDS             18694..19404
                     /gene="budA"
                     /locus_tag="IWO09_001000"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0263609.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            19534..20031
                     /locus_tag="IWO09_001001"
     CDS             19534..20031
                     /locus_tag="IWO09_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0263610.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            20216..20476
                     /locus_tag="IWO09_001002"
     CDS             20216..20476
                     /locus_tag="IWO09_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263611.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(20838..21077)
                     /locus_tag="IWO09_001003"
     CDS             complement(20838..21077)
                     /locus_tag="IWO09_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263612.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            21363..21677
                     /locus_tag="IWO09_001004"
     CDS             21363..21677
                     /locus_tag="IWO09_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263613.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="IWO09_001005"
     tmRNA           complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="IWO09_001005"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(22648..23421)
                     /locus_tag="IWO09_001006"
     CDS             complement(22648..23421)
                     /locus_tag="IWO09_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0263614.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            23565..23915
                     /locus_tag="IWO09_001007"
     CDS             23565..23915
                     /locus_tag="IWO09_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0263615.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            23896..24612
                     /locus_tag="IWO09_001008"
     CDS             23896..24612
                     /locus_tag="IWO09_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0263616.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            24612..26060
                     /locus_tag="IWO09_001009"
     CDS             24612..26060
                     /locus_tag="IWO09_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0263617.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(26130..26639)
                     /locus_tag="IWO09_001010"
     CDS             complement(26130..26639)
                     /locus_tag="IWO09_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263618.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(26629..27354)
                     /locus_tag="IWO09_001011"
     CDS             complement(26629..27354)
                     /locus_tag="IWO09_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263619.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(27577..29697)
                     /locus_tag="IWO09_001012"
     CDS             complement(27577..29697)
                     /locus_tag="IWO09_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0263620.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            29927..31105
                     /locus_tag="IWO09_001013"
     CDS             29927..31105
                     /locus_tag="IWO09_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0263621.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGMLIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            31121..31879
                     /gene="aroD"
                     /locus_tag="IWO09_001014"
     CDS             31121..31879
                     /gene="aroD"
                     /locus_tag="IWO09_001014"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0263622.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            31902..32387
                     /gene="ybaK"
                     /locus_tag="IWO09_001015"
     CDS             31902..32387
                     /gene="ybaK"
                     /locus_tag="IWO09_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0263623.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(32458..33711)
                     /locus_tag="IWO09_001016"
     CDS             complement(32458..33711)
                     /locus_tag="IWO09_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0263624.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            33829..35178
                     /gene="gdhA"
                     /locus_tag="IWO09_001017"
     CDS             33829..35178
                     /gene="gdhA"
                     /locus_tag="IWO09_001017"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0263625.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            35290..36636
                     /locus_tag="IWO09_001018"
     CDS             35290..36636
                     /locus_tag="IWO09_001018"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0263626.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(36944..37330)
                     /locus_tag="IWO09_001019"
     CDS             complement(36944..37330)
                     /locus_tag="IWO09_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0263627.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT        12480 a         6450 c         7405 g        11022 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
     3721 tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa aataaaatct
     3781 ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa agccccaaac
     3841 tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa aggattattt
     3901 gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa taaaatccaa
     3961 cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag aaaatactta
     4021 catcccatta tgatttcgat catcttgatt tttactttct tagggagctt atcaagtatt
     4081 tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga ggagacaaat
     4141 agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag caacaacgag
     4201 acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga aatcacagca
     4261 gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac atttagtact
     4321 gttgcggcca ctgcgaattt caacggacat accataccag atggatatac agtgatatat
     4381 gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa aactgcgtta
     4441 caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca taatctgttt
     4501 attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta tatgggggtg
     4561 gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag ttattctttt
     4621 acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt ttcaatcggt
     4681 atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg gcagatcgtt
     4741 gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt tcaggtcaat
     4801 gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa aatcggaacg
     4861 tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac tccaacagca
     4921 gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga aagtaacgtc
     4981 gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca agactatttt
     5041 ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc tgcacaagac
     5101 ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc agcgatagaa
     5161 acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg aaatgtaatc
     5221 gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa gacaaatgca
     5281 acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt tgtaccgatc
     5341 gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa tgatctcagt
     5401 attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa gggagttgaa
     5461 gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa aagcggacta
     5521 gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc tgatgtctat
     5581 acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata tgatggaaaa
     5641 gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc gcttactcca
     5701 gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc aaaaaatatg
     5761 gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa agtcaaagca
     5821 gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta taccatctat
     5881 aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc ccaaagtcac
     5941 tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc attggattat
     6001 accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat tagtttaggc
     6061 gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa gttgacgatc
     6121 aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt tgtcttttta
     6181 ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa aaaagcaggt
     6241 gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt ctctttttta
     6301 tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca cctattacat
     6361 ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt caaaacgacg
     6421 gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt actttcgcta
     6481 tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt gtggaagacg
     6541 cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa aatgtgacaa
     6601 aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa gatgagcaag
     6661 gtcgtgacgc tgtttaccga tttacggaaa taaagacttc agattaagta aaagaaaaat
     6721 cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa ttgacgacca
     6781 tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta acaaaaacag
     6841 tagagaataa gcagacagat tttgcagatg gggataaagt cccttatctc atcaccgtaa
     6901 caattcctga gaacatcaac gagataggaa cctatacgat caaagataca gctgatcctc
     6961 agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag attcatacat
     7021 ttcacacaca aaaaataaaa catggttttg tcttatcaga ttctggaaaa gatcttagtt
     7081 cgtttgctgg aaaaaaatta acgatccgtt accaaatgac attgaaagaa aacagtgaaa
     7141 aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta gaaacacatc
     7201 ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg aaacaaaaac
     7261 tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa gaaaacggga
     7321 taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc agtaaagatg
     7381 gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag attaaaccgc
     7441 ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa ggcagttatg
     7501 atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat ccgacgaata
     7561 atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa aatggaggaa
     7621 gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg ttattgctag
     7681 tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac aataggaggg
     7741 aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg ctatcatgct
     7801 gcttcctttc gttctcggcc taggaactgc agaggtttct gcagccgtat cacctacacc
     7861 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
     7921 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
     7981 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
     8041 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
     8101 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
     8161 aacgtcgcca aatatcgtgt taagcttacc ctagaccctg acaaaccttt agacacgatt
     8221 cacctatatg ctaaaaatgt catcaaagaa agcaatatca aaatccagaa agctgggaac
     8281 ttttcagggg tagataagtt agcaggggcg caattccaga tcaaaaattc agagaatcaa
     8341 ttcgtgaccg gttttgatga aaacactggt gtcgctctct atgatcctgc tgagtcagcg
     8401 ttgacattca caactgatac tgaaggatcc atcagtgtca atggattaat agacggcaat
     8461 tatactttag ttgaaacaaa agcaccagat ggttatcaat taaccaatcc acaaaaagaa
     8521 actactttta tagtaaaaaa cggaaaattg caacaaacaa ccggtatcat ccatggcgca
     8581 gatgaagcca atcttgggaa tccaaacggt gtagtcaatc gggaaaatac aactacggat
     8641 aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt tccacttcat caaacaagat
     8701 gtgaacacgc aagaaaaatt agccgatgca gaattttcta ttctaagagc aaaagatcca
     8761 actgttaaat taatgaaaaa aatagcaaac cttgcctcag gagaataccc atatgcttgg
     8821 tcagatgaag tcacaggttc tgactgggaa gaagtgaaat tgatttctga ttcagaaggg
     8881 aaattttcaa tcgaagggct gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca
     8941 gaaggctacg cattacctaa gaatgaagct gcgtacactg aatttacagt agatgatgat
     9001 ccaacaactt ctgatgaaac aacgatcgat aaccaaccta aaggaatctt gccttcaact
     9061 ggtggtatgg ggatcatcgt attcatcttg gtaggtactg ctttagttgg aggagcagtg
     9121 atctatttca aaaaacgcca tcaagaaaca gaagcttaaa ctattggaaa caaaaatcat
     9181 acgataaata aacgatagtg attcaaatgg ggagaaaggt tgtgacaagt ggtcggtctc
     9241 ttggtattcg ctcaacaaac ggcaaaatag gaggaactat tttgccgttt gcttcgctaa
     9301 tactcaggtc aaggcgctga tttcacaacc tcttcatata catattagaa aataggtgga
     9361 catgatgaat aaaagcagaa taagaatact ggacatcttc atggcaatca ttttagtcgt
     9421 tgggatcggg atcttttctt atccttttgt agaagattca ctgaatgatt ttcttgccca
     9481 gcaaatgatc atccattatc aaaagcaagc atcgaataag aattcagagg aaataaaaaa
     9541 acagcaggaa aaaatgacaa agaaaaacca acagttggca gagcaaaacg tatcaccagg
     9601 gataggcagt ttcaaccaag cagttgatgc aaaagcatta aaagatttac ctagtaatgc
     9661 cttttttatg gaacatatgc ttggagtcat cgaaatccca aaaatcaatg tcagcttacc
     9721 tatattcgat cagacaacgg aaatattttt gcaaaaagga acgtcattat tggaaggaag
     9781 ttcttatccg actggcggga aaaacaccca tgccgttctt tctgggcatc gtggactccc
     9841 agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa ggagaccagt ttttcatcca
     9901 gatcaatgga aagacattgg cgtatcaagt agaaaaaatc caagtggtct tacctgacga
     9961 agtagattct ttgggtatcc aaaaaggcag agatctcgtg actttgctta cctgcactcc
    10021 ttatatggtc aatacacatc gactgcttgt gacggggcat cgtattcctt atcaagcaaa
    10081 agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa aaatggaaat tttttatttg
    10141 gtttatcggt atactacttg gatcaatcgg cttagtttgg ctactcatcg cttatttaga
    10201 ttctttggcc atcgctaagc gaaactatcc tttatctttt tatgtaaaga acacaaacgg
    10261 acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc aacggcaaac actatattac
    10321 tagagaaaaa gttccatttg tgaaagcttc tgatgaatac ggactagtga gattttctga
    10381 tttaaaaggc aggaactatc gcttgcaaca tgaggagctc ctgcttaaaa tccatgtaaa
    10441 acataaacat tcgaaacagt tttcaatgaa attgaaaaaa ggaagatata aactgcgaaa
    10501 agaaaaagag gtgtactatt tgattgagaa agaatgaatg aagatgtcca aaaaagcaga
    10561 gggaacagac taaatgaaaa aagagagaag ctgacgaaat ggacgtgtaa actgctattt
    10621 ttggtttact atctggaacg ccagcttcta tttttttatg tttcagaaga attttattat
    10681 tatagtataa cttttgaatt agtttccata acatttggag caaggggtta aaccacgagc
    10741 ttgagcttcg cttaaagtgg caggggtata cgtaccattt ccacatttat gtgtatggta
    10801 ttttgaaccg gtcggtgtga catagacagt ttgttcatta ttttgctcag gagctgcggc
    10861 ttgtgcctgc tgagaagcct gttcagctgc tgcttgctgt cgcgcctgtt ctgcttgttg
    10921 ctgtgcgatt gcttcttgcc tcgcctgttc tgcttgttgc tgtgcgattg cttcttgttg
    10981 cgcttgttct gcttgttgtc gtgcaatttc ttcttgcttt ttccgttctt cttctgcttc
    11041 ttttgctttg attgtggaat caattgcagc aagacggctt gtcaactctt gtttgttttc
    11101 aggtagcgct tggatagctg ctgaagcagt tgcatagttt tcttttgtag gatttgcttc
    11161 agcttgtgac aaagcagatt ccgcttctgt cacttttttt tctagtgcag ctttttccgc
    11221 tttttgtttc tcttcttctt ttttcttttc aatttgttct ttcgatgaat ctgccgtact
    11281 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
    11341 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaacg caactaattt
    11401 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
    11461 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
    11521 tcccgtgttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
    11581 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
    11641 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
    11701 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
    11761 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
    11821 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
    11881 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaacaa
    11941 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
    12001 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
    12061 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
    12121 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
    12181 acggacgtca aagaatagtc gccgccagat agaaggaaat tagcagttga aaaagctgcc
    12241 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
    12301 gtcttattac cgcagatgca agctttttcc acacctcttt cttgtcattt acagactcaa
    12361 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
    12421 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
    12481 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataac
    12541 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
    12601 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
    12661 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
    12721 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
    12781 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
    12841 attttgcata attgaaggta gccaattcat taacccaaaa tagccggcaa tttggacagt
    12901 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
    12961 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
    13021 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
    13081 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
    13141 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
    13201 caaggacata cacgccccat attctcctcc cgctcctaca cctgcgataa agcgaaagag
    13261 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
    13321 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
    13381 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
    13441 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
    13501 ctctaaagca attcccgccg atgtcgatac taataccttt cgctgatacg ttgtcatttt
    13561 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
    13621 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
    13681 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
    13741 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
    13801 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    13861 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    13921 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    13981 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    14041 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    14101 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    14161 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    14221 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    14281 caactgaagg agttagaaga gtaactagga acagaactgt ttgtacgcga aaaccgcaaa
    14341 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    14401 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    14461 tacgatcggc tgtgtggaag ctgatagttc gaatacattg gcactattac tagaaaagtt
    14521 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    14581 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    14641 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    14701 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    14761 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    14821 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    14881 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catcattcaa agcgagtgac
    14941 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    15001 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    15061 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    15121 ttgtattttt tcatttttaa atcaaaaaaa taaagagatt tacataactt gttaaataag
    15181 cgcttacaaa aataagaaga acggaaatga atcagctata ataaccgttg ttctggattg
    15241 taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga aagaaaaaaa
    15301 gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat tctgctgatt
    15361 gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc attcaaagca
    15421 aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca tttagagaat
    15481 tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac agctttttta
    15541 agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta cgggttttct
    15601 aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac gattgctgtc
    15661 ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg tttaacagat
    15721 acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag
    15781 caatattaca gtagtatccc agattcttta tgtgagagtg cgcggatcga tggcttatca
    15841 gagtggggaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat tgccagtttg
    15901 acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat ttatttgtct
    15961 tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct atttgatgca
    16021 gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc ttttatattt
    16081 ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa ggaataggag
    16141 gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt ttattggttg
    16201 tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta tatagcagct
    16261 gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc tttacttttt
    16321 attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa agaaaatagt
    16381 ttttcaccag tacgtttgtt cataaagatg tttcgccaat tttggctaaa gggatttttt
    16441 tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt cttttttata
    16501 cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg tttatcagtt
    16561 tcctttagca ccaactgctg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa
    16621 gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt
    16681 ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta
    16741 ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg
    16801 gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt tttatgtaaa
    16861 aactgtcatt ctttttcatt ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct
    16921 atacttttgt atgaaacagg tttagaaact gtatcactta tttgtgatta tttctaacta
    16981 ataattaaaa ataaagcata aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt
    17041 tcgaaatctg tagtagaaag tttgattaat catcatgttg attatgtatt cggtatacca
    17101 ggtgcaaaaa tcgatggtgt atttaatgaa ttagaggatc aaggtcctga attaatcgtg
    17161 actcgtcacg aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat tacaggagag
    17221 ccaggagtag ttatcgcaac aagtggtcct ggtgctagta acttagctac aggtttggta
    17281 acagcgacag cagaaggtga cccagtttta gcaattgccg gacaagtaaa acgaagcgat
    17341 ctattgaagt tgacccatca aagcatggat aatgcagcac tgttccagcc aatcacaaaa
    17401 tacagtgcag aaatccaaga tccagaaaca atttcagaag tgattgccaa tgcttatcgt
    17461 atggctaaga gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga tgtagtggat
    17521 gcacctgtcc aaggtaatgt catcaagcca ttgagcgatc ctaaattagg ttctgcttct
    17581 gcagatgata tccgttacct agctgaacgc attagagaag caaaactacc ggtactatta
    17641 gtaggtatgc gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag
    17701 acagcattgc ctgttgtaga gacattccaa gcagctggag tgatctcacg tgaattagaa
    17761 gcgcatttct ttggtcgtgt gggtttattt agaaaccaac caggagatat gcttttgaaa
    17821 cgaagtgatc tagttattgc aattggctat gatccaatcg aatatgaagc aagaaactgg
    17881 aatgcggaaa aagatgcgcg gatcattgtg atcgatgaag cgccagctga aatcgatcct
    17941 tttatgcagc ctgaaagaga attgatcggt gatatctcag ccactcttga tttattgaca
    18001 ggttcattag agcctcaaca agtgtcagaa gatgcaaaag aatatttagc atctcttcaa
    18061 gcaaaattaa cggagcggga cattgttcaa tcaaaaggag aagcaggtat cttgcatcct
    18121 ttagaagtta ttaacacgct tcagtcaaaa gtaacagatg atatgactgt taccgtagat
    18181 gtaggtagtc attatatttg gatggcacgc cactttagaa gttatgaacc tcgtcatttg
    18241 ttattcagta atggtatgca aacgcttggt gttgcattgc catgggcgat ttctgctgca
    18301 ttggtgcgtc caaatacaca aatcgtatca gtttccggcg atgggggatt tttattctct
    18361 gcccaggatt tagaaacggc ggtacgtaaa aaattgaata tcgttcattt gatttggaat
    18421 gatggacact acaatatggt agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt
    18481 gtagactttg gtccagttga ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc
    18541 cgagctacaa gtgtagagga attagaaaaa gctttagaag aaggatttgc gacagaagga
    18601 cccgtaatta ttgatattcc gattgattac cgtgataatg agaaattggg agaaacaatc
    18661 ttaccagatc aattctatta aggaagtgtc gacatgactg aaaaaatctt ataccagcac
    18721 gggacattag gagctttgat ggccggcttg atggatggaa cagaaacgat tgctcatata
    18781 ttagaaaaag gaactctggg attgggaaca ttgcatggtc ttgatggtga agtcatcttt
    18841 ttagatggcg tagcttatca aggaagatca gatggctcag ttgcccaatt agatggaagt
    18901 gaacttaccc cctatgcagc aattacagat tttacgccag atactagttt ttctgtctca
    18961 aaaactgcag atggagaaca attgaaaaaa gacatattag taagagaagc cggggaaaac
    19021 ctatttttgg cagtgaagat aactggatta ttcaaaaata tgcacatccg tatcatgccg
    19081 aagcaagaaa aaccttacag aagactagca aaaatttcag agtctcaacc agaattccaa
    19141 caaagcaata tccaaggaac attagtcggg ttctttacgc ctgaattatt ccaaggagtt
    19201 gctgcagcgg gatttcacct gcattttatc gatgatacac atacatttgg cggacatgta
    19261 atggattttg aagtggctga aggaaaagtt gagattagcc gcatctcgtc ccttgtacag
    19321 cattttccag ttgatgatcc gacttttgtt cacgaaacga ttgattatgc tgatttagca
    19381 gcagaaatcc aacaagcaga atagaaaaag acagcgagat gcgttcaagg tgattaacct
    19441 gacgtatctc gtttttttga ggaggaaacc tagcgtttac aaaaaaactg agctataatc
    19501 ttctccatag atcaaaaagg agcggttttt atcatgtgga cagaaaaaca gaaaaaagat
    19561 gcatacgaaa ttttattatt acagcaacaa ggattattag aagcagaaga taactggtta
    19621 gcaaatttag caaattcatc tgcattgttg aatgagactt tgcctgagac tgtctttgca
    19681 ggatattatt tattcgatgg taatgaattg attttaggac cgttccaagg gcgagtttct
    19741 tgcacacgta tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca
    19801 ttgatcgttg atgatgtcaa aacccatgca aattacattt cctgcgattc agccgcacaa
    19861 tcagaaatcg tagtgccaat ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat
    19921 tgcggagtaa cgaaagggta tgatgaaatc gatcaagcat ttctagaaaa atatgtagat
    19981 cttttattag caaattttaa tataaatgct ttttcattta ttaataacta aaatcgtcta
    20041 ttgaaacaaa aaagaagaga aatcaagaat aaaaagagcc ggaaagacag tggtcatttc
    20101 tgtttttcgg tttttttatt tttttataaa atcacaagtg ttattgattt tcacaatcat
    20161 attttaatgg tattatcatt ttgaataatg aaaaaacatt aaggatggat gaaatatgaa
    20221 taacgaaact atcattttag gaaaaaaata tatttgccaa cctattggct tgaaacaaaa
    20281 agtggttgga gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg tagacgaata
    20341 tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg caaaatatac
    20401 tcacgtttat gagttggaaa caacagaata tctatataac tatagcaaca aatatgaaca
    20461 tactttagtt tcataaagat ttaaataaag tgccttcaaa agcgagaaga ccaagctttt
    20521 gaaggcactt tatttagttg gcagtgattg ttcatgattt ttgatttatt caaaaggttc
    20581 gcctagagaa tctgtctctg taacgaggcc tggtggagaa actcgataaa aacctatact
    20641 gccagaactg tcctattttc ggatagacaa actagtagtt ttaaaaagat catccgatcc
    20701 aatcatttca aaaaatgcaa aactgatatc aggatcattt agctcaggct tgttatcgta
    20761 aagcatttgt aaaggttgca tttagttagg agtaaacgcg aaactatcat cattatttgc
    20821 tgaagaagat gaagaagcta attcagagtt tgtttgaaat aaaggagacg tgctgacagt
    20881 ttctttaccc tgttttttgc agaggtagaa acttttatat cggaagtttc taccggagaa
    20941 tttttctcat ttgtagaaat tgttgaatcg atactattcg aaatttgcgt atctgaaggt
    21001 atgttctttg atgcagaagt cccacaagca gtaagtaaca ataaatttga taaactgata
    21061 aacagaattc ttttcattat gtacacactc cttatagtat cgtttagcat acacctatgc
    21121 tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct attagtctta
    21181 gtaatacata tatggagagg gtgatatccc agttcaaacc agatacattg gttaccatct
    21241 gtattaacga ttatgcctct aaacgctttt tttatgaaaa aatttataag tgaaaacgta
    21301 ggtatgatat tatctagagg taattttttt gggtatttaa atagttgcta ggaggatagc
    21361 ttttggattt ttacagtaat tttatcttaa taatagcaat attattactt ctaaatattt
    21421 ggttttttga taagagtaga aatgcaggga taggttttag aaccaaacga agtacgtctt
    21481 ctgaaaaaaa gtgggtatat tcacagacta ttttttacgg aggggttatt tcaatcagtt
    21541 tactttcttc aacactttat tcttttaatg ttatagacgt ttccatgtca aattttatat
    21601 ctattattgg aatccttatt tctgcaatta ttacacaact acttcttgtg tttgaagaga
    21661 agtcaaaaaa taattaaagc aattatgcct tttagtacag gaattattac ttcttacaaa
    21721 tgtatagcga atgtgaagcc tgctgtttta attgttctag aaaaactttt gagtgaacaa
    21781 ttattttatg ttaggatgaa ttaatttctc tagagtttat aattaaaaaa cttatttgaa
    21841 ggaacatgct taaaccataa ttgtgttttt ccttccatca gcataaaagt tcacctcaaa
    21901 aagagtgtac gaacaaaagt tctttttgta aaccttaaaa tagattttcc tgaatacgta
    21961 ttggggattt ctaaatgttt gaatgttttt ttgaatgcaa aatcaatgca aaaaattttg
    22021 tatttctcta cttttctctc cttgaccaaa ataaaaaaac gttgatttaa caacgttttt
    22081 catacatgtt tatctgtctc aaccatacat atatggagac ggcgggagtc gaacccgcgt
    22141 ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag gattcacttt
    22201 acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt tttcaactta
    22261 cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag cacatgggcg
    22321 atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag ttgttttcgt
    22381 ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg aaacgcaatt
    22441 caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag ggttacgtgt
    22501 atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa aagcgaatca
    22561 tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg attttcttaa
    22621 gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca atgcgtggac
    22681 aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta atccgtcata
    22741 ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat cattcggacc
    22801 atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca gacgtttgac
    22861 acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac cattccgata
    22921 aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag ataagatcag
    22981 caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa catctgaatg
    23041 aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga ttttctgatc
    23101 gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt tttgacattc
    23161 ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga aagaaccatt
    23221 catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt ctgcttctgt
    23281 acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca tagcttttgc
    23341 cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat caaagaaagt
    23401 gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag tttatcttaa
    23461 caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt ggttcagtca
    23521 acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt tttttcagta
    23581 ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca aaaggaaatc
    23641 tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat tggcggctgg
    23701 ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca ttgatcggct
    23761 attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt ctgctagtat
    23821 ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat gacctggaaa
    23881 ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt ttctgaaatg
    23941 atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga tggaaaagaa
    24001 gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact agacctgaat
    24061 ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc aagtactgtg
    24121 ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg cttagagatg
    24181 ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc tcgaatgaaa
    24241 gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagcagt ttcagatgat
    24301 cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga agcgtatctg
    24361 gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt tactttagca
    24421 aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg ggattatcag
    24481 tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca aaaaatcgaa
    24541 aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt cgatgattct
    24601 ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttttgcgga atggtcgcgt
    24661 tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg attgaagaaa
    24721 acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg caaacctatg
    24781 ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg ctgctgacag
    24841 agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac gaacgtgtca
    24901 tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa tggaaaaatc
    24961 tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga caagcacagg
    25021 cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga gcacttgtag
    25081 ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg aacctgttca
    25141 aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt gcagcattcc
    25201 aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat ggtaattttg
    25261 atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat gatttcaata
    25321 aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa aatcgtcgaa
    25381 gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca atcaacggac
    25441 ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac gcgattaaat
    25501 tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg gattatgaaa
    25561 aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca gaaacattga
    25621 aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca ttgacattga
    25681 aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa atcatggtca
    25741 atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt actttagaaa
    25801 aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg tctgaacaac
    25861 aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa aatacaaaat
    25921 atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg cataaaggaa
    25981 aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt ttgcctgacg
    26041 tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg atcttctata
    26101 ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg atcaatataa
    26161 ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt ttcttcatga
    26221 gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag ttcatcaaat
    26281 gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt
    26341 actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg agagatatca
    26401 agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac atccaacagg
    26461 acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat gatcgcaaaa
    26521 ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt atgctgtttt
    26581 tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt
    26641 acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga atagactaag
    26701 aatgagacag catccactga gcccaataag agtttgggca aatcccttaa gtatatctgt
    26761 gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt
    26821 aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag tctttaagac
    26881 aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta cgatccacaa
    26941 taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa
    27001 taatttggtt ttagaaagac cagtcaggcg ccaattgatc aattcctgct tgttttgcct
    27061 gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata aactgaatat
    27121 gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg tagatgcaga
    27181 agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt gcaaatggta
    27241 aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac aatagagtat
    27301 tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt tcatactttt
    27361 cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc tttagtgaaa
    27421 tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag aagctgccct
    27481 attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag gagcagcgac
    27541 aataattttg tcgctgctcc ttttaaattt attttgtcat tcggaagaac ttgtggtcgt
    27601 tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg
    27661 ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc
    27721 taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata
    27781 gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt
    27841 ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc
    27901 agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc
    27961 atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat
    28021 ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc
    28081 gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac
    28141 acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc
    28201 agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac
    28261 tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc
    28321 taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg
    28381 gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa
    28441 ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg
    28501 tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc
    28561 aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc
    28621 gttcccatgg aataccgcac tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc
    28681 ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa
    28741 tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa
    28801 gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc
    28861 atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc
    28921 taaatggata taaatcacat tttttccttt agcgagcccg aacaaatcgt catttggtgc
    28981 tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc
    29041 ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc
    29101 aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc
    29161 ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc
    29221 cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt
    29281 caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact
    29341 ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc
    29401 cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat
    29461 gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa
    29521 taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat
    29581 tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag
    29641 taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat
    29701 aatcttcctt tctgcacttc ttaatcacag aagtgtcatg aaaccatgtt acacagctca
    29761 cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat
    29821 gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta
    29881 tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt
    29941 aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct
    30001 tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt
    30061 tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca
    30121 gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag
    30181 aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga
    30241 cggaatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa
    30301 cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga
    30361 aatcgtagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt
    30421 catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc
    30481 cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg
    30541 atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg
    30601 cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc
    30661 aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca
    30721 gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt
    30781 tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt
    30841 tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga
    30901 cgggatgctt attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat
    30961 gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct
    31021 gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt
    31081 ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggtttggg taaagggatt
    31141 gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga
    31201 agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt
    31261 cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca
    31321 tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga
    31381 acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt
    31441 agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga
    31501 ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc
    31561 agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg
    31621 aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag
    31681 acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa atcttggtaa
    31741 aatttctcgt ctggctggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag
    31801 cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt
    31861 attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag
    31921 taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat
    31981 atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc
    32041 aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg
    32101 tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa
    32161 aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct
    32221 gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct
    32281 atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag
    32341 atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa
    32401 aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta
    32461 tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt
    32521 aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat
    32581 gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac
    32641 aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg
    32701 aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag
    32761 ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa
    32821 gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg
    32881 tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt
    32941 gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa
    33001 atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc
    33061 ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg
    33121 ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc
    33181 atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg
    33241 agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa
    33301 tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa
    33361 tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc
    33421 ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc
    33481 aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg
    33541 acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt
    33601 tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat
    33661 catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc
    33721 tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaaatgttt tgagccaaaa
    33781 gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat gacaaacgca
    33841 acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa
    33901 tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga cactcatccc
    33961 gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt tattcaattt
    34021 cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta ccgtatccaa
    34081 tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag tgtcaatcta
    34141 agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac cggtctgcca
    34201 atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga taatgagatc
    34261 atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc atcgattgat
    34321 gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt cggcgaatat
    34381 aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga tttttgggga
    34441 agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa acatctattg
    34501 aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag cgggaatgta
    34561 gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac atgttcagat
    34621 tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa agaagtcaaa
    34681 gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc tatttatcat
    34741 gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc atgtgctact
    34801 caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat caaaatcgta
    34861 gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca tgaagcaggc
    34921 gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc tgctttagaa
    34981 atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga gcagctggat
    35041 caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta cggcgacgaa
    35101 aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa agcaatggca
    35161 gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca ttttgtttat
    35221 ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa cctataagga
    35281 ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa
    35341 caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt acgggaaggt
    35401 actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta tgacaaagaa
    35461 gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga agtattagtt
    35521 gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt cttgaatcac
    35581 acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt ctttgccgga
    35641 aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga tcgcgacttt
    35701 tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc gttccgtgta
    35761 ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg tatctacgct
    35821 acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg ttgggaaact
    35881 ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt tggtttgtta
    35941 ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc tgatgcaagc
    36001 aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc agcaatgcgc
    36061 aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga accaggaatg
    36121 caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa
    36181 ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg acaattcatc
    36241 caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca
    36301 atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca aggaaaagaa
    36361 gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac agacggtgac
    36421 gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg ttatacaatg
    36481 tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa cccattcgac
    36541 caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa accaggcttt
    36601 gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc gctagattta
    36661 aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact
    36721 caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt gtcacagtcc
    36781 cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag gactttaaga
    36841 acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat cagtaagcaa
    36901 aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat aggcctaatt
    36961 tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct atgatccgct
    37021 tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca ttggcagagt
    37081 caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca gcataagcat
    37141 ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg atatcagttt
    37201 ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa gtagactgat
    37261 agtagtccca acctttccat cctataaaag caatcaatat gataacgatc aaccctaaaa
    37321 tttttttcat gaaagatctc cttttttatt tttatta
//
LOCUS       DACECJ010000019        34689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.019, whole genome shotgun sequence.
ACCESSION   DACECJ010000019 DACECJ010000000
VERSION     DACECJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.019"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            644..1333
                     /locus_tag="IWO09_001020"
     CDS             644..1333
                     /locus_tag="IWO09_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0263628.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1320..2528
                     /locus_tag="IWO09_001021"
     CDS             1320..2528
                     /locus_tag="IWO09_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0263629.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2822..4093
                     /gene="serS"
                     /locus_tag="IWO09_001022"
     CDS             2822..4093
                     /gene="serS"
                     /locus_tag="IWO09_001022"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0263630.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4642..6126)
                     /gene="guaB"
                     /locus_tag="IWO09_001023"
     CDS             complement(4642..6126)
                     /gene="guaB"
                     /locus_tag="IWO09_001023"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0263631.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6631..7326)
                     /locus_tag="IWO09_001024"
     CDS             complement(6631..7326)
                     /locus_tag="IWO09_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0263632.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7416..8516)
                     /gene="ychF"
                     /locus_tag="IWO09_001025"
     CDS             complement(7416..8516)
                     /gene="ychF"
                     /locus_tag="IWO09_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0263633.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8525..8716)
                     /locus_tag="IWO09_001026"
     CDS             complement(8525..8716)
                     /locus_tag="IWO09_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0263634.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8737..9627)
                     /locus_tag="IWO09_001027"
     CDS             complement(8737..9627)
                     /locus_tag="IWO09_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0263635.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9614..10381)
                     /locus_tag="IWO09_001028"
     CDS             complement(9614..10381)
                     /locus_tag="IWO09_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0263636.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10393..11106)
                     /gene="rsmG"
                     /locus_tag="IWO09_001029"
     CDS             complement(10393..11106)
                     /gene="rsmG"
                     /locus_tag="IWO09_001029"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0263637.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11338..13239)
                     /gene="mnmG"
                     /locus_tag="IWO09_001030"
     CDS             complement(11338..13239)
                     /gene="mnmG"
                     /locus_tag="IWO09_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0263638.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13256..14653)
                     /gene="mnmE"
                     /locus_tag="IWO09_001031"
     CDS             complement(13256..14653)
                     /gene="mnmE"
                     /locus_tag="IWO09_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0263639.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14997..15335
                     /locus_tag="IWO09_001032"
     CDS             14997..15335
                     /locus_tag="IWO09_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263640.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15503..16261)
                     /locus_tag="IWO09_001033"
     CDS             complement(15503..16261)
                     /locus_tag="IWO09_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0263641.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16279..17097)
                     /locus_tag="IWO09_001034"
     CDS             complement(16279..17097)
                     /locus_tag="IWO09_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0263642.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17094..17450)
                     /gene="rnpA"
                     /locus_tag="IWO09_001035"
     CDS             complement(17094..17450)
                     /gene="rnpA"
                     /locus_tag="IWO09_001035"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0263643.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17568..17702)
                     /gene="rpmH"
                     /locus_tag="IWO09_001036"
     CDS             complement(17568..17702)
                     /gene="rpmH"
                     /locus_tag="IWO09_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0263644.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18225..19559
                     /gene="dnaA"
                     /locus_tag="IWO09_001037"
     CDS             18225..19559
                     /gene="dnaA"
                     /locus_tag="IWO09_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0263645.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19757..20887
                     /gene="dnaN"
                     /locus_tag="IWO09_001038"
     CDS             19757..20887
                     /gene="dnaN"
                     /locus_tag="IWO09_001038"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0263646.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21116..21358
                     /gene="yaaA"
                     /locus_tag="IWO09_001039"
     CDS             21116..21358
                     /gene="yaaA"
                     /locus_tag="IWO09_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0263647.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21345..22469
                     /gene="recF"
                     /locus_tag="IWO09_001040"
     CDS             21345..22469
                     /gene="recF"
                     /locus_tag="IWO09_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0263648.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22466..24412
                     /gene="gyrB"
                     /locus_tag="IWO09_001041"
     CDS             22466..24412
                     /gene="gyrB"
                     /locus_tag="IWO09_001041"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0263649.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24432..26903
                     /gene="gyrA"
                     /locus_tag="IWO09_001042"
     CDS             24432..26903
                     /gene="gyrA"
                     /locus_tag="IWO09_001042"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0263650.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27139..27438
                     /locus_tag="IWO09_001043"
     CDS             27139..27438
                     /locus_tag="IWO09_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0263651.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27488..28000
                     /gene="ssb"
                     /locus_tag="IWO09_001044"
     CDS             27488..28000
                     /gene="ssb"
                     /locus_tag="IWO09_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0263652.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28026..28262
                     /gene="rpsR"
                     /locus_tag="IWO09_001045"
     CDS             28026..28262
                     /gene="rpsR"
                     /locus_tag="IWO09_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0263653.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28405..30378
                     /locus_tag="IWO09_001046"
     CDS             28405..30378
                     /locus_tag="IWO09_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0263654.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30384..30836
                     /gene="rplI"
                     /locus_tag="IWO09_001047"
     CDS             30384..30836
                     /gene="rplI"
                     /locus_tag="IWO09_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0263655.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31110..32477
                     /gene="dnaB"
                     /locus_tag="IWO09_001048"
     CDS             31110..32477
                     /gene="dnaB"
                     /locus_tag="IWO09_001048"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0263656.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33257..34549
                     /locus_tag="IWO09_001049"
     CDS             33257..34549
                     /locus_tag="IWO09_001049"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0263657.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10565 a         6744 c         6851 g        10529 t
ORIGIN      
        1 ctacacattt ggtttgtctt actttgttca gttttcaaag gtctacttca tcaggagtgt
       61 ttcccgattc gtttttggct gccgtagcaa cttttatatc atatcagaga gacaagtaat
      121 tgtcaactct tttttaaaag ttttttcgat ttcgtttttc gaaatcttcc agcaactcat
      181 ttatcataac ttatcgtttg ttatttgtca agaacttttt tgaagttttt ttcgatagaa
      241 tcaagcttcg caagtggttc ttttttaacg actcagttat cttatcatgt catttccaac
      301 atgtcaacaa cttatttttt attatttaat aagttattgg tgttcttttg aacaaataat
      361 aatatatcaa catcacatgt gaaagtcaat aagaaaatgc ttatttatca acatttctag
      421 cttacatgat cattggttag ttctggtcct ttttcgttcg tctttataac tccgagagac
      481 gtaaaatcct tttgttttca gtttaatgtt catgttcttg tcttattcac accaactaga
      541 tgtctcacct tctaaaattt tccttaacaa agtgctgctt ttatctattt ctgctttttt
      601 ttgctagaat aatagtaaca atatgactct ggaggattta tttatgaaaa ttttgattgc
      661 tgacgatgat aaagaaatcg ttgaattatt aagtatctat gttcacaatg aggggtatga
      721 agcagtaaag gcttatgatg ggaaagaagc attatctaaa atcagaacaa tccctgatat
      781 tgaattactt attttagata tcatgatgcc tgagatggat gggatgcaag tagtgaaaga
      841 gctacgaaaa gaatcccaaa taccaatcat tatgctgaca gcaaaaacta ctgatatgga
      901 taagatcaaa ggactggtcg ctggtgcaga tgattatgtg accaagccat ttaatccgtt
      961 agaagtaatg gctagagtaa agtctttatt acggcgtaca aaaatgcaga ttgccccaga
     1021 tcagcctgat gtattagaag taaatgcatt aatcatcaat aaagactctc atgaagttaa
     1081 aacaatcgat gggaaagaaa tccagttaac agctttggaa ttcggtatcc tctacttgct
     1141 tgctagtcat cctaatcgag tatttagcgc ggatgaaatt tttgagcgtg tttggaaaca
     1201 agaaagtatt gtctctgcta aaactgtcat ggttcacgtt agtcatttaa gagataagat
     1261 tgaagaagca actggtggtg aaaaagtcat tcaaaccgtt tggggagtgg gctataaaat
     1321 tgacgcaaga tagtaaacga gcaactgaaa aacgccgtcg gatcaatttg acttcaaaag
     1381 aaatcagcga attgttagcg gaaggaatca tcacgattat ccttttgctt ctattaaatg
     1441 tttctattct ggttgttatc agttcggtga tcaacagttc accttctttg acgaatgcga
     1501 tttgggattc aaagaatatt tttgcagaac gattgaacac tgatttgttc tggaacggcc
     1561 ggaattttat tattcccttt ttctttctat tagatattgg tgttttgtat tggcgcttga
     1621 ttcgaagata tcgccaaatg caattgcggc atatcatcag cgaacttcac tacatcgcaa
     1681 acggaaatta tgatcatcgt atcccttttg aactgagtgg tgacttaagc cgagtggtaa
     1741 caagtatcaa tggattggtg gatagtaccg tagcagcgat tgaagacgaa cgtaaaatcg
     1801 aaaaatcaaa agatgaattg atcacgaatg tcagtcatga tatccgtacc ccactcacgt
     1861 ccatcattgg atacctagga ctgatagaag atggccagta tcactcagaa gaagatcttt
     1921 taaagtatac gcatactgct tatatcaaag caaagcagat gaaatcttta gtggacgact
     1981 tatttgaata cactaaggta cgccagcctg ctgtcccggt gaatttttct gcctttgaca
     2041 tgatccagtt gatcgaacag ttagcagctg attttgagtt ggaagcttct aaaaaaaata
     2101 tccaaatact tgttcaatca aaagtagact cccttatcat ggatggcgat accgaaaaac
     2161 ttgtacgtgt cttcaataat cttttgacga atgcgttgaa atacggaaaa ggagcaacga
     2221 agatcgtaat cgaagtagaa cgtatcggtt cagaagttgt tgcaacagtc aaaaataacg
     2281 gagcaatgat cccacaacag gcaatcgata atctattcga ccgtttttat cgtgtagaag
     2341 aatctcgttc acaagcaact ggaggaactg gacttggttt agcaatcgct caaagcattg
     2401 tggctttgca cggcggatac atttatgcaa aatcagataa gcaatggact tctttcatca
     2461 tgcaccttcc aatcaagaaa aatgaaaaat tacctattga tacacaggaa gttattgacg
     2521 aaagctgaaa tattcgctat tcttattaca attattagaa taaatgaaaa acacagaacc
     2581 cagtacactt tattcttttt cagagagctg atggcaggtg taaatcagca aaggataatt
     2641 tggaagttcc agttcgttta cgtgcccttt cgaaataaga aaggtcggtt caacagccgt
     2701 tatgtgttca acaagtgcag ctgacgctga acttgggtgg taccgcgaat ttcagactga
     2761 ttcgtcccaa gaataatggg atggatcagt ctttttttat tttcaaaaag gaggaaatca
     2821 tatgttagat gtaaaaatga tgcgacaaaa tattgaagaa gtacaagaaa aattgatgac
     2881 tcggggcgta aaaaaagaaa cattagctga attcatggaa ctagatgaaa atcgtcgtaa
     2941 gctactagtc aaaagtgaag aattaaaaaa atatcgaaat gatgtttctg ctgagatcgc
     3001 ccaattgaag cgtgcaaaag aagatgcttc tgctaaaatc gctgaaatga aagaagtcgg
     3061 tggcaacatc aaagcattgg atactgaaat tgctgaagtg gatgaaaaat tgaaacaaat
     3121 tgcaactact ttgccaaacc ttccccatga ttctgtgcca gttggaacag atgaagagga
     3181 caatgtagaa atccgtcgtt ggagcgaacc acgtacattt gcttttgaac caaaaccaca
     3241 ctgggaaatt gcagaaaatc ttgatatttt agattttgaa cgtggcgcaa aagtagccgg
     3301 tagccgtttt gtctattaca aaggattagg tgctcgatta gaacgtgctg tctacaactt
     3361 catgcttgat cagcatattt acgaacacgg ctatactgaa atgatcactc cttatatcgt
     3421 caacagtaaa gcgatgtttg gtacgggaca atttccaaaa ttcaaagaag atgttttcca
     3481 attggagaat actgatctaa cattaatccc aacagctgaa gtaccattaa ctaactatta
     3541 taatgatgaa attttagatg gtgcagattt acctatttat ttcactgcac taagccctgc
     3601 ttttcgttct gaagccggaa gtgctggtcg ggatacacga ggactgattc gtcttcacca
     3661 attcaataaa gtagaaatgg tcaaattttc ggatgtagag cattcttacg aagaattgga
     3721 aaaaatgaca gctgatgcag aagatatctt gaaaaaattg catttgcctt atcgtgtgat
     3781 ggccttatca actggagaca tgggcttttc tgcagctaaa acttatgact tagaagtatg
     3841 gatacctgca caagatactt atcgtgaaat cagttcttgt tctaactgtg aagatttcca
     3901 agcacgccgc gcaatgatcc gttatcgcga taatgatgga aaaatccact atactcatac
     3961 tctgaatggt tcaggacttg cagttggccg tacagtagct gccattttgg aaaactacca
     4021 aaatgaagat ggttctgtca cagttccaga agcgctcgtt ccttatatgg ggaatctgaa
     4081 agttattaaa taaagaggct gtgaaaaagc cgcttagttt cgagtattaa gaaaaatttt
     4141 gtgaaaatgg ctttccacat ttttgcataa attggactta ataccgagaa actggctttt
     4201 gaacaccatt tatcgagagg ttgtgaaatc agcgaattaa cctgagtatt agagaaaaaa
     4261 atggaaaaat agtccctcat attttaccgt ttgttgaact aataccgagg gtcagctgat
     4321 tgaacaccgt ttagtggaga ggtcgtaagc ctacccccca gctcctagtt acaaggacaa
     4381 attcaaaaaa cagctcttca tattttatga atgttgtact tgtgattcgg aggtggcaag
     4441 ggaacactgt ttattcaaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
     4501 gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ttcttatttc cgaaggagtt
     4561 gcttctactt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
     4621 gtcccacgct ctttaatgtt tttaagattc aactgaatag tttggtgctt cttttgtgat
     4681 ttgtacatca tgagggtgtg attcttttaa tccactgccg ctcatttgga taaattgcgc
     4741 ttcatcacgt aattgctgta gattggctgc cccaacataa cccattcctg aacgcaaacc
     4801 gccgatcatt tggaaaataa tatctgcaac acttccttta taagcaaccc gaccttcgat
     4861 tccttctggt actaatttat ttgcttcatt gacactacct tggaagtaac ggtcttttga
     4921 acctttttcc atcgcgccta atgagcccat acctcggtat gttttgaatc gacgtccttg
     4981 gaagatttcg aattcacctg gtgattcatc tgtccctgcc aacatacttc ctagcatcac
     5041 tgcatgtccg ccggcagcta atgctttgac gatgtcacca gaatatttga tccctccgtc
     5101 tgcaataatc gctttgccat attgacgagc aactgatgct gcatcataaa tagctgtgag
     5161 ctgaggtaca cctacaccgg ctaccacacg tgtcgtacaa atcgatcctg gtccgatacc
     5221 aacttttacg acatctacac ctgcatcata taaagctttt gtcgcttcag ccgtcgcaac
     5281 gtttcctgca atcaatgttg catctgcaaa agttgaacgg atttcttgaa tttttcggat
     5341 cacacctgca ctatgtccat gagctgtatc gatgatgatt gcatctgccc ctgcttctaa
     5401 taaagcattc gcacgctcaa atgtatcgct tgttacacca acggctgctg ctacaagcaa
     5461 tcgaccatgt tcatcttttg ctgcatttgg aaattcgatc actttctcga tgtctttgat
     5521 cgtgatcaat ccgcttaaac gaccttcgtt atctacgatt ggtaattttt cgattttatg
     5581 tttttgcaaa atcttttctg catcttttag agaggtacct actggggcag tcactaagtt
     5641 gtcttttgtc atcacatcag caatcggcat gtgataatct gttacaaaac gcatgtcgcg
     5701 gtttgtaatt atccctacca attttctgtt ttcaagtgtt tcaacgattg gcacaccact
     5761 gatacgatat ttgctcatca attcttctgc gtctgctaca agatgctgtg gcgttaagaa
     5821 gaacggatca atgattacgc cactttcaga acgtttgact tttcttactt catccgcttg
     5881 ctggctgatc gtcatgtttt tatggatcac acctagtccg ccttgtcttg ccatagcaat
     5941 ggccatcttg ctgtcggtaa cagtatccat acttgcactg atgattggaa tattcaactt
     6001 aatattttta gctaattgta cgctcatatc cacttcgttt ggtaacacgt gactttctgc
     6061 tggtatcaat aagacatcat caaaagtaaa tccttttttc gtgaactttg tttcccagtt
     6121 ggacattttt agtaccactc ctctaattta gtttatctat taaaataatt gattttttca
     6181 tttaagtcaa tgatctactt ggaatcaagt tagctttctc atagaatacc aatataaagc
     6241 gtttacatct gtgtatatta aaagagattg taaaaaagct gtcctgcatc aagtaataag
     6301 aacagccaat caaaaacagc ttctcatgtt tttgaattgg ttgggtttat taccgcagat
     6361 gcaaactttt gaacaccgtt tagtggagag gctgtgaaaa agctgcttag tttcgagtag
     6421 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaattggac ttaataccga
     6481 gaaactggct tttgaacacc gtttatcgta aaaagggact gtgacaagac ttttgtcaca
     6541 gtcccttact attatttaac tatggataaa caaaatgagg ttctttacta cccctagata
     6601 tctagcaaaa tagctgatct ttttgctagt ttatcttctt gggacggata aacttccaat
     6661 aatcccgtat tttttcttca agtagtaacg gattgctaaa gcagttgccc cgataatgat
     6721 aactactact ggatctaata ctggattgat aactggtggc aatagtgttg tcgcagaaaa
     6781 cagtacgacc caaagaagtg tcgttccacc taggataagc aatgtcttac ccattcctgg
     6841 tttcttgctt cgatctgctc cgggatattc atattgatag atgtatttgt acatcatata
     6901 aaagacccag ccaccgattg cagaacctag aacgagtgta gaaaagccat aagctggcgt
     6961 tgttccacgt gaaaacaatc cggtaatcgc aatcatgata cttaatagac caagcaacag
     7021 caacgtatta tctaaccaca tcaataaagg ggtggatgaa ttttttactt ctgctggctt
     7081 attgatgatt gcttccgttc tttcagagac tgtcccaaac aattgacggg ctgttcttcc
     7141 gcctttttgt tctgcaacta aagtaggcag aatgtcatga agggctttta ctttttcttc
     7201 ttcagacaaa ttcgctgctt ctaaagattt tttcaaatca aaaatgtact gttgatttct
     7261 tttggttaat tttgtttcta aactattgtt ttcttttacg attgctcgta attgttctgg
     7321 ttccattatc taatagtcct ccttagccgt tccttttctc aacggtcgtc acaaaagaca
     7381 ttctaccata ttctacagct agtttgatag cccttttaca cattaaagcg gaataacatt
     7441 acgtcgccgt cttgtacgac ataatctttt ccttctaatc gaacacgtcc agcttctttt
     7501 gctgcatgca tgtttccgta atgatccaag tcttcaaaag agacagtttc tgcacggata
     7561 aatccgcgtt cgaaatctga atggatgatt cctgctgctt gtggtgcttt gattcctttt
     7621 ttgaatgtcc aagcacgtac ttcttgttcc ccagcagtaa agtaagtagc taagcctaac
     7681 aaatcataag cagcacggat caactgatcc aaacctgatt cttcgattcc taaagcttct
     7741 aaaaattccg ctttgtcttc ttcatctaat tctgcgattt cttcttctgc acgagcactg
     7801 accacgatca cttctgcatt ttcttctgca gcaaagttgc gcacttcttg tacataatga
     7861 ttcgcgtctg catctgctac gtcatcttct gagacattgg ctacataaag aattggtttt
     7921 gtcgtcaata aaaatagtga tttaacgatt ttttgttctt cttcagtaaa ttcgatcgtt
     7981 cgggcagatt ttccttcttc cagtacaggt ttgattttat ccagtacagc tagttctgcc
     8041 actgcatctt tatctttggt tttagcaact tttgctacac gagtatagcg tttgttgata
     8101 gattctaagt ctgctaacac tagttccaag ttgatcgtat caatatcttc tagtggatca
     8161 acacgtcctt ctacgtgagt aatattatca tcatcaaaac agcgaacaac gtgacaaata
     8221 gcgtctactt ggcggatatg gcttaagaat tgatttccca aaccttctcc tttacttgcg
     8281 ccttttacga ttccagcaat atctgtaaat tcaaaagtcg tcggaacagt ttttttcgga
     8341 tgtacaagtt ctgttaagcg ttgcaaacgc cagtcaggta cttctaccat ccctacgttg
     8401 gggtcgatcg tcgcaaaggg atagtttgct gcttctgctc ctgcttttgt gatcgcatta
     8461 aacaaagttg attttcctac gttgggcaac ccaacaattc cagcagtcaa tgccataaat
     8521 ttattcactc tttctctgca tttttttcaa ttactttttt taatttcttt tcaaaatctc
     8581 gacgggtcat catgacgata tgtccgcaat tcatacatct gattttgata tctgcgccca
     8641 ttcgaatgac ttcccagcga ttcgtctggc aagcgtgagg ttttttcatt tcaacgatat
     8701 cgtgcaagtc gtacataaaa atagcctcct ttttgtttat tcttcatcaa attgaatatt
     8761 caagatatca agaattcgag tcaaatcttt ttgagaaaga tattcaatct cgatcttacc
     8821 tttcccattt ttttcttgga tagctacact tgtaccaaat ttatccatca aacgatcttc
     8881 gctttctcgg atataatatg gtttttcctt tgctagcctt gggatttttt tcttttcttt
     8941 tccatcattt tgatttaact cagaaacaag ctgttccagt tggcggacag ttaaattttc
     9001 agttactgca cgattggcta atttcaagat caatgtttta ttttttaaac ctaacagcgt
     9061 acgggcttgt cccatagata aacgctgatc ttgtaccatt tccttgacaa gatccggtaa
     9121 agaaagcaag cgcaaataat tggcgatata aggtctgctc tttcctaaac gactagctac
     9181 ttctgcttgt gttagtttca ggtttttcat cagcatctca tatgcttctg cttcttctaa
     9241 cggattcaga tcttctcgct gcaagttttc cagcactgct acttgcatca tcgcttcttc
     9301 gtcaaagtct ctaacgatag cagggatcgt ttctttttca gctagtttgg acgctcgaaa
     9361 acgtcgctcc cctgcaataa tctcatatcc ttttactgcg gaacggcgta cgatgatggg
     9421 ttgaaagaca cctgattgtt caatagaatt agctagttcc tgcaaagata cttcttcaaa
     9481 tgttttacgg ggctgatacg gatttggtcg cagctcattc aatggtagct ggacaacggt
     9541 atcatctttt acatccaccg tctctaagtt ttctaaatct tgaaacagtg catcgattcc
     9601 tcggcctaat cctttacttt tcttcacgac ttaccacttc ctttgccaat gcttggtaga
     9661 cttcagctcc tcgagaacgt ggatcgtagt cgatgatcgg ttttccatga ctaggtgctt
     9721 ctgataaacg aatatttctt ggaatgatcg tttcgtaaac tttttcgcgg aagtatttac
     9781 gtacttcttc tacgacttca tttcccagat ttgttcgtgc atcatacatt gtcagtaaga
     9841 ctccctcgat ctctaattct ggattaaagt gtttttggac taaacgaacc gtattcagta
     9901 attggcttaa tccttccaat gcgtaatact cacactgaac aggtatcaaa atcgaatcac
     9961 ttgctgtaaa tgaattgatt gtcagatggc caagagaagg cgggcaatcg atcaaaatat
    10021 aatcatactg actgcttact tctgctaaag atccttttaa tcttgattct cttgccatca
    10081 ttgacgtcaa ttcgatctct gctcctgcta attggagagt cgcaggcaca atactcaaat
    10141 tttcgtgttc agtgatcaaa gttgcctcat caattggcaa ttcattgact agaacatcat
    10201 agatgtctct tgtcacatca ggttttctga tcccgacacc actggtagca tttccttgtg
    10261 catccatatc cactagcaat acttttttgc caaggctagc aagacaagca cctagattca
    10321 ccgtcgtcgt tgttttgccg acgccacctt tttggttcgc aacagatatt atttgtgcca
    10381 tctcttctcc tcctatttga ttggctgctt attaggcagt ccgggctttc ttgggtattt
    10441 ctttggggtt ggtttctttt tttgaataac gatgatatgc cgttgatcag agacattcgg
    10501 caaggtaacc gcctcttctt tgaggaattt accacctaat aaagcaatcg cttgttttgc
    10561 ttctgtcaat tcctcttcac tttttgctgc tttcaatgca taaaaatagc cttcttgttt
    10621 agccaagggc aaacacaatt ccgctaacac attcaaacga gcaaccgcac gagcagtgac
    10681 aaggtcaaat gatgcacgaa agtgaggatt ttgcccaaat gtttctgccc ggtcatgata
    10741 taaggaagca tcgattccta ataatcgggt taactcagat aagaaggtta ttcgtttatt
    10801 cagagaatca acgatcgtta tctgcagttc tggaaaggca atttttaaag gtatgctagg
    10861 aaaccctgcc cccgaaccaa catcgcataa agataaaggt tgcgtaaagt ctacttcaaa
    10921 tgctaatgta agtgaatcgt aaaaatgctt cagatatacg tctttttcat ccgtaatagc
    10981 tgtaaggttc atcttttcgt tccattcgac taatgtccga taataaatag cgaattgatt
    11041 catttgatga tcggtaagct ctatcccttt ttctgccaag gattggcgaa attcttctgg
    11101 tatcataaat attcctttca ttgtgaaaca gcaattgttt cacagattct ctccctactt
    11161 taacgcaaaa tctctagaca tgcaatcctt ccatatggat tcttaaaaat ttgaaaagta
    11221 agaaaagccg atagactttt tctttctagt atgactcata aaatatacac agaagagaga
    11281 actagctttg agcaatttct gcaaaaacta gttctctctt ctatttatta ttacgtatta
    11341 cgctggttca ctgactttcg caatcttccc ttgttcgata tagaccatta ggatacttag
    11401 gtctgcaggg tttacgccac tgattcggct agcttgggca attgtttctg gttggatttt
    11461 ctgcaatttt tgttttgctt ctgttgctaa tccgttgatt gctgcgtagt cgatgttttc
    11521 tggaatcttt ttcgcttcca tgcgttttaa tttttctact ttttccatgg cttttttgat
    11581 gtagccttca tatttgattt ggatttctac ttgttcgatt tcttttcgtg tcaattcttc
    11641 attttctgga ataaaccgta gaagttctgc ataagtaatc tcaggacgtc ttaaaaagtc
    11701 gcttgctagt acaccatctt ttaatggtgc cgcattaatc gttgctaaaa agtcttggac
    11761 ttcttctgtt ggtttgattc gtacattcat caaacgtttg atttcttgtt caactgcttc
    11821 ttttttcttc aaataattct tgtattgatc ttcttttact aagcctacac ggtgacccat
    11881 ttctgttaat cgcaaatctg cattatcatg acgtaaaagc agacggtatt cagcacgtga
    11941 agtcagcaaa cgataaggtt cgttcgttcc ttttgttacc aaatcatcga tcatgacacc
    12001 gatgtaaccg tcacttcgtt tgatcactaa aggttctttt ccctgaactt tcaaagctgc
    12061 attgataccg gcaatcagcc cttgtccagc cgcttcttca taaccgcttg taccatttgt
    12121 ttgtccagct gtataaagat tttcaacgac tttagtttct aatgtcggac ggagctggtg
    12181 tggctctact acgtcatatt cgatagcata tcctgtacgc atcatttcta ctttttctaa
    12241 tccagcaata gaatgaagca tgttgacttg aacatcctct ggtaaagaag tagataaacc
    12301 ttgaacatag acttcttccg tatttaagcc ttctggttcc aaaaataatt gatgacgtgg
    12361 tttgtctgca aaacgcacga tcttatcttc gatggatgga caatagcgag cgcctacccc
    12421 ttcaacgatc cctgtaaaca ttggggcacg atgtaagttg tctcgaataa tttggtgagt
    12481 cgtctcacct gtataggtca gccagcaaga ttcctgatct agtttatagg cactatctgg
    12541 tgtgctgaag ctaaaatgat tcggctcttt gtcaccaggc tgctcttctg taacagaata
    12601 gtcgatcgta cttgatttta ctcgaggcgg tgttcctgtt ttgaaccgtt cgatagcaaa
    12661 gccaagttct tttagatgat tcgctagtcc aatagaaggt tgagagttat tcggacctga
    12721 tgaatatttc aattcaccga taatgatttc tccgcgaagt gctgttccgg ctgtaataac
    12781 aactgctttg gcccggtagc tcgctcctgt ggacgtcact actccatgac aaacaccatc
    12841 ttcgacgatc agttcttcga cgattccttg tcttagcgtc aaatttggtt ctttttcgat
    12901 cgtatgtttc atttctgttg aataagcatg tttgtctgct tgtgctcgca atgctcggac
    12961 agctgggcct tttcctgtgt tcaacatacg catttggata taggttttat cgatattctt
    13021 acccatttct ccgcctaacg catcgatttc tcgaacgaca acgcctttag ctggtccgcc
    13081 tacagaagga ttacatggca tgaatgctac catgtccaaa tttattgtca gtaataatgt
    13141 ttttgatccc atacgtgcag cagcaagtgc agcttctgaa ccggcgtgtc ctgcgccaac
    13201 aacgatcacg tcatacgcat ctgcttcata atgttccaat cgtttgttcc tccttttatt
    13261 ttcctaaaca aaattgactg aataattgag tgattagctc atcttgtaca ctgtcgccga
    13321 caacttctcc tagatagtcc cagcaacgcg tcatatctat ttgaacgaga tccacaggca
    13381 ttcctgaatc aatacctgca atgacttctt caagtgagag ggaagctttt tctaacaacg
    13441 cgatatggcg ggtattagaa acataagtag cgtctctttc tcctgtttct ccgctgaaga
    13501 ataaatcaga aatagctgat tccaaacgat ctagtccgtc gtttttagct acggaaattg
    13561 aaaaaactgg ttcattttcg atcaatttct tcaatttttc ttgttccagc tgtgctggca
    13621 aatctgtttt atttagaaga atgatccgtt tcaatccact tgtcgcttcc aataattgtt
    13681 catcttctgc tgtcagtggt tcgctttggt ttagaacaag taagatcaag tctgcttctg
    13741 ctaatgcttt tcggcttctt tcgaccccga tccgttccac cacatcttct gtttcccgaa
    13801 ttccagctgt atcgattagc ttgagcggta ccccccgaac attgacatac tcttcgatta
    13861 catctcgagt cgttcctgca atatccgtaa cgattgcttt ttcttctctt aacaaatggt
    13921 ttaacaaact tgatttgccg acatttggcc ggccgatgat tgccgtactt aacccttccc
    13981 gcagaacctt cccttgcttg gatgtagcaa gtagcgcttg gattctttgt tggatcatct
    14041 gcgctttttc aagcaataac ttcgtagtca attcttcgac atcatcatat tctggataat
    14101 caatatttac ttctacttgt gctaatgtct ctaaaatctc ttgtctcaat gagcggatca
    14161 aagcagacag attgccgtct aactgattaa gagctagtcc cattgctttg tctgtttttg
    14221 cccgaatcaa atccatcaca gcttctgcct gtgaaaggtc aacacgacca ttcaagaatg
    14281 ctcgttttgt gaattcgcct ggctcagcta atcttgcacc ttctcttaac aatagctgca
    14341 aaatttgatt gactacaacg atcccaccgt gacaattgat ttctactaca tcttcccgcg
    14401 taaatgtctt tggtgctctc atcacggaga ccatgacttc atcgacaagc tgattgctct
    14461 tgggatcaac gatatgtcca taatggatcg tatgacttgg tacttctgat aatcgtttgt
    14521 ttccagattg ataaacttta tctgctagtt gcacagcttg atcgccactt aaacgaacga
    14581 tactgattgc accttctcca ggtggggtag aaattgccgc aatcgtatca aattctaaag
    14641 taatttcagc cattttttca cttccttttt cattttgcgc acaaaaaaag tgcccactcc
    14701 accctttatg cacaataaac ggtgaacttc gcactttgac tgcttgttct tgattaaatt
    14761 ctggtcatag attatcacac ttgctataag gaatcaagtg tttaaaacaa ttagatgaag
    14821 ttgagcctgt tctgcttcca ttaccttata tttaattgtc cttggatgtc tctttgcgtt
    14881 ctttggttta ttcgaagaga agatactgta taaacaaggc tttatctttc ttaaaaaaaa
    14941 cggtacacta aatggtagcg ctatctaaac tatacacttt tgaaaggaga aaatatgtga
    15001 gcccgttcag atctgctgca ttggaagaag aaagtgaccc tcggatcaac gatgtcgttt
    15061 tatctacagg gaatatcaac cagaaatata tcgtccgcga tatcgttttt gttgtggaat
    15121 gcttagaagc tgatttgttt gacccatctt ttcccaaaga tgaccttttc ttaacaacga
    15181 agaggaaatt aaagacgaag gtacttgaat atggtgcgaa tgcagtaatc aactgtcatt
    15241 ttgagtatca gcaaaaaaaa cgcgacggaa agtattttct ggaacttttt gcttatggaa
    15301 cagtgatcca atttatccaa acaacgattg gttaaatgaa acagacaaaa aaagcggatc
    15361 aaaactgaag acagtttcga tccgcttttt tctatcaaag ttagtgacta cttcatcgta
    15421 tactctgata agaatgattt ctagtaaaat atacttggtt tgaaaaactt tgtcaacgtg
    15481 atttattcaa aaagtaatgg aatcaataat attttttagc aggttctact actaaataac
    15541 gataaggttc ctcaccttcc gaatgtgttt ttacatgttc atctttgctt aatgcagcat
    15601 ggatttgttt acgttcaaat gcaggcattg gttctaagaa aacaggctgc cctgtccgtt
    15661 ttactttttc agccgtgcga tcagctaaac gctgaatgat ctcttggcgc ttttcacggt
    15721 aattgcctac attgactact acagacagct tatttcttgc taaacgatgg atgaagactt
    15781 gtgccagata ttgcaatgca ttcagtgttt ttccatgttt cccaatcagt atgccttgct
    15841 tttgtgtttc taaatggaaa acaaccatat tatttgattc tctttccatt ttgacaagag
    15901 ctggagcatc aagctgtttt gaaatttcag taagataaac tgccaattcc gttaaagctg
    15961 cttcatcacc tagctcactt acttcttctg ttttttcttc aagaatcgtt tcgttgacta
    16021 cctcgatctc agctgttggt tcttcttcca aaatttcttc tacagtctgt tctactgttt
    16081 cagaaacttt ttcgctgata cttggctgga tcgaaagccg tgcattcttt cgacccattc
    16141 ctaggaaacc tttttttcct tcttccagta tttcgatttc gacttggtct ttggccaaac
    16201 ccaaatcagc cagacctagt tggacagctt cgtctattgt tgcagcttca taaatcggca
    16261 ttagcattac ctcccctatt atttccgttt tttcttagga ttcattgctt tcttaagagc
    16321 acgttctctt tcgcggattc tccgtgcttc ttcttcacgc tcttttcgaa ttttgaaagg
    16381 gttgttgatc agcagcgttt gtacaacttg gaaggcattg gaaactaccc agtataatga
    16441 aagaccgctg gctaaattga cacccatcac taaaatcatt acaggcatca cgaaattcat
    16501 gatcttcatt gatgcattcg attcaatttg actcatgcta gataaatacg tacttgcaaa
    16561 agtaaagatt gctgctaaaa tcggcaaaat aaatgttgga tccgcgttcc ctaagttcaa
    16621 ccataagaaa tgcccttgtt tcaactctgg tacacgtgaa atagattgcc ataacgccat
    16681 caagattggc atttgtacta ataaaggaag acaaccagca tatggattga cgccattttc
    16741 tgcatataaa cgctgctgct cttctcttaa cttactttgt gtttccggat ctcttgaact
    16801 atattgttgc tgcagtgctt ttaatttcgg ttgtaattct tgcgttttac gcatgctctt
    16861 tgtttggaaa tgcatcaacg gcaataggat cacgcggatg atcagtgtaa ataaaatgat
    16921 ccctatccct gcattaccaa atgacaacgc cttgatcgcc tcagcaaagt aataaacaat
    16981 ataacgatcc caaattcctg tactctgtgc gctgacttcc ccagttccgc aaccggataa
    17041 gacgatgacc aaactaacga taccggccat caaaagtaaa cgtttgtatt tcttcactta
    17101 ctcttttcct ctctgtcgat tatttttgca agttttaaga cgtggactaa attggagcga
    17161 acctcatcat aagtcaaccc cttcacactt ggtctagcaa tcacaataaa atccaactcc
    17221 ggatcaattt cttcttttat agaataaact gctgctcgga tttttcgttt gacttcattg
    17281 cggtttaccg cgttgccgac cttttttcct acagataaac caactcgaaa atgaggctgt
    17341 cccggttttt ccaaacggta aacgacgaat ttgcggttag caaaagaatc tcccgtatgg
    17401 aacacggctt gaaactctcg ttcttttttt actcgatacg cttttctcat tagttttctt
    17461 tccttttcta tctctcactt acgctcataa tcataccata attacgattg tcctttcatc
    17521 atttccctaa aaaagagtat aaaaaaaacc actgaaaccc cagtggccta agctgaaaga
    17581 acttttcttc ctttgcgacg acggctagct aacacacgac gtccattttt agtactcata
    17641 cgtttacgga atccgtgaac tttttgacgt ttacgtttat ttggttggta tgttcttttc
    17701 atatattcca cctccaaaat gttcttatct attttataaa cacagacata ctttgatagt
    17761 ataacgacaa agtagtgatt ttgtcaatat aaaatagaag atcgattgtt aaaacttatc
    17821 cacaagatta tttttttcta tgggaatcct atgtggaaaa agttatgcac agggattatc
    17881 cacagataat aaaaggctgg ttttacacag aaaacacacg tgtaaaaaaa gtaatccaca
    17941 atcaggcgat ttcctgtgta ttccgtctcc taactattgg tatatctgta ttttttatcc
    18001 acagacgcaa gaggaaatct attggttttt acgagttttc cacctgtgca ttagtggtgt
    18061 ggaaaacttt ttttgacaac tgtgtttttc tcttttttct ttttgtcgat tgtgtggata
    18121 attgtaatta aaaaattttt ttcttcggtt tctgtggaaa actattgaaa aaaatgctaa
    18181 attagtacta gttattcact ttttaatgaa ggaggaggcc ttatatggta tccctcgatg
    18241 ctttatggaa tgaattaaaa gcaacatacc aaaaggattt atcgcctgct agctataata
    18301 catggatcga aacagcgaac cctcgaaccc ttgatcaaaa ccagctcgtt gttgaggttc
    18361 ctagcaagat tcataaagaa tattgggaaa aaaacctggc gacaaaaatc gttgaagtcg
    18421 gttatatgtt atcaggaaat gagattatcc ctcgctttat tactggcgaa gaagcagagc
    18481 aagaagaagt tatagaagaa aaaaatccaa aagttgtggc acctagtccg ctaaaaaaag
    18541 ccatgctgaa ccctaaatat acctttgata cgtttgtcat tggtaaagga aaccagatgg
    18601 ctcatgcagc agcgctcgtt gtagcagaag atccaggttc tatttataac ccgctcttct
    18661 tttatggagg cgtgggactt ggtaagactc acttgatgca tgcaattggt catcaaatgc
    18721 tgcaaagtca gcctaatgcc aaagtaaaat atgtaagcag tgaaactttt gctaatgatt
    18781 tcatcaattc gatccaaaac aaaacagcag aagagttccg tcaagaatat cgaaatgttg
    18841 atttactgct agtggatgac attcaatttt ttgcagaaaa agaagctacg caagaagagt
    18901 ttttccatac tttcaatgcc ttgtacaatg aagggaaaca aatcgtacta accagtgatc
    18961 ggttgccaaa cgagattccg aaactgcaag aacgccttgt ttctcgcttt gcatgggggc
    19021 tgtctgttga tattacaccg ccggatcttg aaacaagaac agcgatttta cgtaaaaaag
    19081 cggcggctga acgtctagag atcccagatg acacactaag ctacatcgca ggacaaattg
    19141 attctaacat tcgtgaatta gaaggtgcgc ttgttcgagt gcaagctttt gctgcgatga
    19201 atagtgaaga tatttcgact agtctagctg cagacgcact gaaaacgtta aaatcaggaa
    19261 aaggacatcc tcaattgtca atcctgcaga tccaagaaga agtggcaaag tattatcaca
    19321 tccaattgaa agatctcaaa ggaaagaaac gcgtcaaatc tattgttgtt cctagacaaa
    19381 tcgctatgta tttggcacgt gagctcacag ataattcttt accgaaaatc ggagctgaat
    19441 tcggtggcaa ggatcataca acggttatcc atgctcatga aaaaatccag cagctgatgg
    19501 attctagtgt cagtatgcaa aatgaagtat cagaaatcaa aaacttttta ctaaattaat
    19561 ttgtcgattc tatcaactga aaatagcatg tggataaaag aaaaaaacgt tcaaaagtta
    19621 tacacacttt ttccacagct gttttttcgt ttctctctta cgttttttga gttttccaca
    19681 gaatcaacat gccctattac ttttattatt ctttttaact aataataaat aaacaaacat
    19741 tcaaaggaga tacatcatga aagttacttt aaaccgagct agctttatgc aggaattgca
    19801 aactgttcaa cgagctattt caagcaaaac cacgatccct attttgacag gtgtaaaaat
    19861 cacactgaca caagaaggtt tgactttgac ggggagcaac gctgatatat caattgaaac
    19921 ttttttgtct gttgaaaacg aaaaagcaaa tatgcaaatc gaatctactg gttccattgt
    19981 tttacaagca cgtttcttta gcgaaatcat tcggagactt cctgaagaaa catttacttt
    20041 agaagtttta gaaaataaac aagtagcgat cacttctgga aaagcgaatt ttatcgtaaa
    20101 tggattagat gcagataact atcctcatct tcctgttgtc gaaagccata accagatgaa
    20161 attacctgta cacgtattga ctaaactaat caacgaaaca gtttttgctg tctcacaaca
    20221 tgagagtcgt ccaatcttga caggtgtgca tttcatttta tctgataatt ctttattagc
    20281 tgtagctact gattctcacc gtctaagtca acgcgtgatt ccagtagaac aagcggctga
    20341 tcattttgat attgttattc ctggaaaaag tttgatcgaa ttatctcgct cattaacaaa
    20401 tgaagaagaa atcgtcgaaa tcagcattat ggaaaaccaa gtgttgttca aaacagaaac
    20461 gatgtatttc tattcccgtt tgctagaagg aaactatcca gataccaatc gtttgattcc
    20521 ttcaagtttt aatacagagg ttgaattttc tgttcctagc tttttagcgg cgatcgaacg
    20581 ggcttcttta ctttctcatg aaggccgtaa caacatcgtt cgtttatcta ttcgtccaga
    20641 tgcggttgtt ttatacggaa attcgccaga aatcgggaaa gtagaagaaa gcttgagtta
    20701 tacagcaagc agtggcgatc cattagatat ttctttcaat ccggattata tgaaagcagc
    20761 gttgcgtgca tttggagata tgagcatcaa agtgaaattt atctctgcta ttcgtccatt
    20821 tacattggaa ccaacggaag atggcgttca atttattcag ctgattactc ctgtacgtac
    20881 aaattaacaa tgattttcaa tgttcttgaa aatttaattg aaaagaaata gaaaaaataa
    20941 aattttaaac agaaggccct aaaatctctt ttaaggcttt ctgtttattt ttgtttaaat
    21001 cacctttttt agattaaatc gtcttatatg gaaaaatata tggataaatt tgttttattg
    21061 cgaaaaaaaa ggtataatag agtatacttt taataatgaa aatgagggat gggcattgaa
    21121 acaacaagtc attctaaata cggaattcat gacgttgggc caaatgttga aagaagtcac
    21181 agttatcggt agtggcggac aagcaaaatg gtatttggca gaaaacactg tgttagtcga
    21241 tggcgaacca gaaaatcgtc gtggacgcaa actttatccc ggcatgatgg ttgaagttcc
    21301 agaagtagga acttttttta tggtaaaaaa aggaaacgac gataatgagg ctgaataagc
    21361 tgtatttaaa aaattatcga aattacgaag agctgaacat cgaattttcc aagaacctcg
    21421 tcatcttttt aggagaaaat gcgcaaggaa aaacgaatct tttggaaagt atctacgtat
    21481 tagctatgac gcgaagtcat cggacaagta gtgagcagga attgattggc tggactgacg
    21541 atcaggcgat gattcaagga gagatcacaa aaggttcatc cactcttcca ttagaaatct
    21601 tattatctaa aaaaggccga aaaaccaaga tcaaccatat cgaacagaaa aagttaagta
    21661 cttacgtggg acaaatgaat gtcattttat ttgcaccaga agatctttct ttagtaaaag
    21721 gcagcccgca gaccaggcgg aagtttttag atatggaatt aggccagatc aatccaatct
    21781 atctttatga tcttgtccaa taccaagcaa ttttgaaaca aagaaaccag tacttgaagc
    21841 aattgaatga aaaaaaacaa actgatttgc tttatttgga tattttgtcc gaacaactag
    21901 cagcgtttgg cagcaaagta ttgaaagcaa gagcacagtt cgtcaagcga ttggagtttt
    21961 gggctaattc tttgcatcag caaatcaccc atcaaaaaga gcagctggaa atcgagtatc
    22021 tcaccgcagt cgattcttta gaaacacata cgcaagagca aatccaagaa caatttctcg
    22081 cacttttgaa ccaaaataaa aagaaagacc tttttcgggg aacaacgaca gttggtcctc
    22141 atcgtgacga tctttccttt tttattaatc agaaaaatgt gcagacttat ggatcgcaag
    22201 gacagcaaag aacaactgca ctaagtgtca aattagcaga aatcgatttg attaaagaag
    22261 agacagggga atatccaatc cttttgttag atgatgtgat gagcgagctg gatgataatc
    22321 gtcaactcca tctgctagaa acaatcgaag gaaaagttca aacgttctta acaacaacga
    22381 cacttgacca tgtaaaaaat aaaatgactg ttgaacctga gattttttat gttcaacaag
    22441 ggaaactaaa aggagaagaa actgaatgac agaagaaaga agtttagtag aacgcgctaa
    22501 agagtatgat gccagtcaga ttcaagtact tgaaggactt gaagccgttc gtaaacgacc
    22561 tggcatgtat atcggttcga ctagttctga gggtctgcat caccttgttt gggaaattgt
    22621 cgataattcg atcgatgaag tattagcagg ttttgcaacg aaaatccatg tgattattga
    22681 aaaagacaac agtatcaccg ttattgacga cggtcggggg atacctgtag atatccaggc
    22741 aaaaactgga cgtcctgcag ttgaaacggt ctttactgtt ttacatgctg gaggaaaatt
    22801 cggcggtggt gggtacaaag tttctggtgg acttcacgga gtaggatctt ctgttgttaa
    22861 cgcgctttct acacagcttg atgtcaaagt atacaaagaa ggaaatgtgt actatcaaga
    22921 atatcgtcgt ggagctgtag tggacgactt aaagattatc gaacaaacgg atcgccatgg
    22981 aacaacggtt cactttacgc cagaccctga aatttttaca gagacaactg agtttgattt
    23041 ttcaaaattg gctacccgga tccgtgaact tgctttcttg aaccgcggga tgcgtatatc
    23101 gattgaagac aaacgagaag aagaaccagt catcaatgaa taccattatg acggcgggat
    23161 taaaagttat gtcgaatact tgaatgcaaa caaaaccgtg atttttccag aacctgtata
    23221 tttggaaggt gaacagcaag atatcgcagt tgaagtctct atgcaatata cagacggcta
    23281 ccattccaat atcatgagtt ttgccaacaa tatccatacg tatgaaggcg gaacacatga
    23341 atcaggattc aagactgctt taactcgtgt gataaatgat tatgcccgaa aacaaaagct
    23401 gatgaaagaa aatgacgaca acttgaccgg tgaagacgtg cgagaaggat tgacagctgt
    23461 tatttctatc aagcatcctg accctcaatt cgaaggacaa acaaaaacaa aattgggcaa
    23521 ttccgaagta cggacagtca ctgatcggtt atttagcgaa catttcatga aattcttgct
    23581 ggaaaaccca agtgtgggcc gtcaaattgt tgaaaaagga ttactagctt ccagagctcg
    23641 tctagcagcg aaacgtgcgc gtgaagtaac gagacgtaaa ggtgctttag agattagtaa
    23701 tctgccagga aaattagcgg actgctctag caacgatcct gaaaaatgcg aattgttcat
    23761 cgtcgaagga gattcggccg gcggatcagc taagcaaggt cgtaatcgag agtttcaagc
    23821 catcttgcct attcgtggga aaatcttgaa tgttgaaaaa gcaagcatgg acaagatctt
    23881 ggcaaatgaa gagatccggt ctttatttac tgctatgggt acagggtttg gagaagattt
    23941 tgatgtatcg aaagctcgct accacaaatt agttatcatg actgatgccg atgtcgacgg
    24001 cgcgcatatc cgtacattgc tgctgactct tttttatcgt tacatgcgtc ctattgttga
    24061 agctggatac gtctatattg ctcagccacc tttatacggg gtaaaacaag ggaagaatat
    24121 tacgtatgta caaccaggaa aaaatgcaga agaagagttg aaacaagtcg ttgcttcact
    24181 tcctgcctca ccaaaaccaa gcgttcagcg ttataaagga cttggagaaa tggatgatca
    24241 tcaactatgg gaaacaacta tggacccaag caatcggatg atggcccgcg tcagtgtgga
    24301 cgatgctatt gcagcagatc agatatttga aatgttgatg ggggatcgag tagaaccccg
    24361 tcgtgcattc atcgaggaaa atgcacacta tgtgaagaac ttagatattt aatcatgaag
    24421 gaggaaaaag aatgagtgaa gaaatcagag aaaacatcca tgatgtcaat ctaaccagcg
    24481 aaatgaaaga ctccttcatc gattatgcga tgagtgttat tgtcgcacgt gcacttcctg
    24541 atgtcagaga tggtctgaaa cccgttcatc gccgtatttt atacggcatg aacgaattag
    24601 gtgtaacacc ggataaagcc cataaaaaat ctgccagaat cgtcggagat gtcatgggta
    24661 aataccatcc tcatggagac tatgcgatct atgagtcaat ggtacggatg gcacagccat
    24721 tcagctaccg ttatatgcta gtggatggtc atggaaactt tggttctgtt gacggtgacg
    24781 gtgccgccgc tatgcgttat accgaagcac ggatgagcaa aatcgctaca gaaatgctgc
    24841 gtgatatcaa taagaacact gttgatttcc aaagcaacta tgatgataca gaaaaagaac
    24901 cagttgtact acctgctcgc ttcccgaatc ttttagtgaa cggaacaacg gggatcgccg
    24961 taggtatggc cacgaatatc ccacctcata acttgtctga ggtagtagcg gctatcgatt
    25021 tgctgatgga taatcctgat gtgacgacca atgaattgat ggaagtatta cctggaccag
    25081 attttccaac aggtggtttg gttatgggaa aatcaggaat ccgaagagcg tatgaaacag
    25141 gtaagggttc tattaccgta cgtgcaaaag ttgagattac tgagatgcca aatggtaaag
    25201 aacggatcat cgtgacagaa ttgccttata tggtcaataa agcaaaattg atcgaacgga
    25261 tttctgaact gcacagagac aagcgtatcg aagggatcac agatttgcgg gacgaatctt
    25321 cccgtgaagg tatgcggatc gtcattgaca tccgtcgtga cgccagcgca tctgttatct
    25381 tgaataactt atataaattg acatccttgc aaaattcatt tggattcaat atgttggcga
    25441 tcgaaaaagg tgtgccgaaa gttttgagct tgaaacaaat tcttgaaaac tatatcgaac
    25501 atcagcgcga agtcatcaca agacggacag tctttgaaaa agaaaaagca gaagcaaggg
    25561 cacacatttt agaaggtttg cgtattgctt tggatcatat cgatgaaatc attgcgatta
    25621 tccgcgggtc taaatcagat gacgaagcaa aagcgacgat gatcgaacgg ttcgatttat
    25681 ccgatcgcca gtctcaagct attttagata tgcgtttacg ccgattgacc ggtttggaac
    25741 gggaaaaaat cgaaaatgaa taccaagaac tcttaaaact gattgaagat ttaactgaca
    25801 ttctggcaag accagaacgt gttacagaaa tcatcaagaa tgaattagca gacttgaatc
    25861 aacgatttgg tgataaacga cgcacagaat tactagtcgg tgaggtctta agtctggaag
    25921 atgaagattt aatcgaagaa gaagaaatcg ttattacatt gacaaacaat ggttacatca
    25981 aacgtttggc aaataatgaa ttcagagctc aacgaagggg tggacgtggt gtccaaggaa
    26041 tgggtatcca caatgacgac ttcgtcaaaa atctggtttc ttgttctacc catgatacat
    26101 tgttattctt tacgaataac ggaaaagtgt atcgggcaaa aggatacgaa attccagaat
    26161 acgggcgcac agccaaagga ataccaatta tcaatttact aggtatcgat tcgagtgaaa
    26221 gcatccaggc aatcattgcg gtgacgggag aagcagaaga aggccactat ctcttcttta
    26281 cgacgcgtca aggtacagta aaacgtactt ctgtcactgc ttttgctaac atccgaagta
    26341 atggtctgat cgcaattggt ttgaaagaag acgatgagct cgtcaatgtg ctactgacag
    26401 atggtcaatc taatatcatt attggaacac ataacggcta ctctgtcact ttcaaagaag
    26461 aagcagtccg cgatatggga cgtacagctt ccggggttag aggaatacgc ctgcgtgagg
    26521 aagattatgt cgttggggca gccttaatga aggaagatca agaagtatta gtgatcacag
    26581 aaaaaggtta cggaaaacgg acaaaagtat cggaatatcc agtgaaaggc cgcggaggta
    26641 aagggatcaa aacggcgaac atcactgaaa aaaatggtcc actagctgga ttgaccactg
    26701 tttctggaga tgaagacatc atgctgatta cagataaagg tgtcattatc cgtttcaacg
    26761 tatcgactgt ttctcaaact ggacgttcaa cattaggcgt acgtttgatg aagatggaag
    26821 ctgatacgaa agttgtcacg atggcagcag tcgaaccaga agctgcagaa caaacagttg
    26881 aagaacaagg atcagaagaa tagccatcaa gactaattgt gtaagctagc aagaatgacc
    26941 gattaaaaaa tctggttatt cctgctggct tttttgatta gttgaatttc tcttaaaata
    27001 ttcgaaactg ttcattctgt attgcttttg accaaaatag agtgtataat cagagcttgt
    27061 gagtagtcta attaattaga taactctcct tgctcttagg aacctaagag ccgaaagtcc
    27121 acagggaggt gaatatcaat ggaaaacacg aaatatgaaa tcatgtacat tattcgtcca
    27181 aacattgatg aagaagcaaa aactgcttta atcgaacgtt tcgatgcaat cttgaaagat
    27241 aacggagcag aagttatcga atcaaaagat tgggaaaaac gccgtttcgc ttatgaaatg
    27301 aacggttacc gtgaaggtat ctatcacatc gttaaagtat cttctccatc atcagcaaat
    27361 gctgtcaatg aatttgaccg tcttgctaaa atcaacgacg atatcattcg tcacatgatt
    27421 gttaaagaag aagcttaatt gttgtttcat gtgaaacaat agaaaacgat gagaggagat
    27481 gactattttg attaataatg ttgtattagt tggacgtctg acgaaagatc cagatttgcg
    27541 ttacacagca agtggtacgg cagtcgcaac atttactttg gctgtgaatc gtaattttac
    27601 aaaccaaagc ggcaatcgcg aggctgattt tatcaactgt gtgatttggc gcaaatcagc
    27661 agaaacttta gcaaactatg ctcgtaaagg aacactttta ggtgttactg gacgtatcca
    27721 aactcgttct tatgataatc agcaaggaca acgtgtatac gtgacagaag tagtagctga
    27781 caacttccaa ttgctggaaa gtcgttctgc atctgaaaat cgccaacaaa gcggcggatt
    27841 ccaaagttct ggtcaatcag ctggcggatt tggcggaaac aataactcta atcaaacatc
    27901 tcaatcatcc aacggtatgc ctgatttcga ccgtgatact tctgacccat tcggctcaag
    27961 ttcaacgatc gacatttcag acgatgattt accattctaa acgaatttga taggagggaa
    28021 atagaatggc acaacaaaga agaggcggac gtaaacgtcg taaagtagac tacattgctg
    28081 ctaaccatat tgaatatatc gattataaag atatcgaatt actaaaaaga tttatttcag
    28141 aacgcggtaa aatcttgcca cgtcgtgtaa ctggtacagg cgctaaaaac caacgtaaaa
    28201 ttactgttgc aatcaaacgc gctcgtatca tgggcttgct acctttcgtt agcgaagact
    28261 aataaatcct aggaattgaa tgactgatcc ttttgatcag ctattcaatt cctttttttc
    28321 tgtaaaaata acctctattc gtttagtcgc tcgaatgacg aaagttatgg taaaatcaaa
    28381 ttagtcatat atgggaggaa aattatggaa aataaaaaca atcatttatc cccgaaacct
    28441 ctcttcctta tcctgtttat attaatcgaa gtattggtag tcttaatttt accatttcgt
    28501 tttgttagcg cttcggtcat tctattgatg aatttatggg gtgttcgcta ttggttgaaa
    28561 atgaaacgtt ggcttatttt aaataaacgt gaaaaaatcc agcaggcttc gactgtagct
    28621 gaaaccaatc tagcttatgt ctcagaagtc atgcctgtag gagtgattgc ttatcagcca
    28681 tcaagccaaa aaatcgaatg gctcaatcca ttagcattga aaatcaaaga tcaaagtgga
    28741 ttaacagagg aagaattgct agatactttt tttactgcaa aagaaaaaaa gaaaaaccaa
    28801 gtctatctag cagataaaac atttcagttt gatatggata cagtaaaggg tgttgtttat
    28861 tttaatgata ttacccaaaa ccagttgtta aaactccatc agacgaacag tcagccggtg
    28921 attggtattt tatctattga taactatgca gatgccattg ataaattgga tgacaaagag
    28981 atttcttatt tgaactcttt cgtcactaca ctagtttctg actggatgaa tgaataccat
    29041 gtctttttca aaagaatcaa cgcagaacgg tttttcttcg tagcacagat gatcgatctg
    29101 aaaaagatga tggataaaaa atttgatttg ctggatcgct ttcgagcaga agctgaaaaa
    29161 cagagctcgc ctatcaccct tagtatgggg attgcttatg gctcaagaac cctcgatgaa
    29221 atcggtggag aagctcagaa taatttagat gttgctttag tccgtggagg cgaccaagtt
    29281 gtcgtgaaag aggtaaaaga aggagcaaaa cctgtttatt atggggggaa atctgctaag
    29341 aatgtcaaac gcacgcgtgt tcgttctcga gcgatgagta ccgctttgcg tcggattttt
    29401 gatgaaacag gcgatgtgtt tatcatgggg cacaagtttc cggatatgga tgctttaggc
    29461 tctgcttttg gtgtcagttg tctggcacgc ttcaaccaga aaaaagctta catcgttatc
    29521 aacgagaacg agatcattcc agatgtagaa cggtgtcttg aagaaatcca taagcatcct
    29581 gaattagaaa tgcaactaat ctctccagaa cgtgccatag agctgaaaaa agaagaggac
    29641 ctcctcgtga tggtggatta ccataaacct tctctatcga tctctcaggg cctatatgag
    29701 caatttgata agattgtgat cattgatcac catcgtcgtg gggaagagtt tccaagtaaa
    29761 cctctgctga cttatatcga atcttcagct tcttcagctt cagagctagt ttctgagctg
    29821 atccaatacc aaagcagcaa aacaaatcgc ttagatcgtt ttgaggccac attattgctt
    29881 gcagggatgg ttgtagatac aaatagtttc aatgtccgta caactgcacg aacttttgac
    29941 gttgccagct atttaaggtc ttgtggagca gacgcatcct tagtacaata tttattgagc
    30001 tcggatttag cgacatactt ggagatcagc aatttgattg ctggaagcga ctatgtgact
    30061 tctgatattg ttgtggcggc agggacagac gaaaaagagt ataatagtgt tatagcagca
    30121 aaaacagcag atacattgct ttcgatgatc aatatcaatg cagctttcgt cattacaaag
    30181 cggacagacg gcttgattgg gatcagtgcc agaagtaccg gcacgatcaa cgtccaaatc
    30241 atcatggaat ccttaggtgg cggaggacac ttcacaaacg cagctactca actggaaaat
    30301 acgacagttg aggaaacaaa acagaagcta tttgctgcaa tcaaagaaaa tatgaatcaa
    30361 atatatgata aggagtaatc caaatgaaag taattttttt acaagatgta aaaggaaaag
    30421 gtaaaaaagg ggacatcaaa gaagttccag caggttatgc acaaaattat ctaatcaaaa
    30481 ataacttggc aaaagaagca acgaaaagca gtatcagtca gttagcagga caaaagaaag
    30541 cacaagaaaa acacgaagct gaaatcttgg aagaagctaa aaagttaaaa gaattcttgg
    30601 aaaaagaaga tactgtagtc gaattgaagg cgaaagcagg ggaagacagc cgtttgtttg
    30661 gttcgatccc ttcaaaacaa attgctgaag ggttaaacaa acaatacaaa atcaaattag
    30721 ataaacgaaa aatcgaatta gaaaatccga tccgttcact aggctacaca aacgtacctg
    30781 ttaagcttca tcatgaggtg acggctacga tcaaagttca tgtcgtacaa gaataacaaa
    30841 tcttgatagc gatgttcagc tggtaagtat aaccagaagg aaaaaagaca aaccgaaatt
    30901 tatctgagaa aacagctgca gcaatgaaag caaaagctgc aaatcgattt cagattgatt
    30961 tcggtttctt ttttgggaac agcaaaatag cataaagaaa acgagtataa taaaagagaa
    31021 ctattttttt attacacttt cggtacgaat gatgactttt ctctcatttt cttgtaaaat
    31081 gaagcaattg aagacaagga gcgatacgta tgaatgaagt gtggcaggat cgaatcccac
    31141 cgcaaaatat tgaagctgaa caagcggttt taggctctgt ttttcttgac gcagaaacga
    31201 tcattgatgc catggagtac atagagccaa aagactttta tcgtcgtggc catcaaatta
    31261 tttttgagac aatgatcgag ttaaatgatc gtaatgaagc gattgacgtg gttactgtca
    31321 aagaccggct ggaacaagcc aatttgttag aagatgccgg tggtttaagt tatctctcag
    31381 acttggcatt agcagttccg acagcagcaa atatcgtcta ctatgcgaaa atcgttgaag
    31441 aaaaatcatt gctgcgtaca ttgatccaga cagcaacagg aatcgttacg aaaggattcg
    31501 agcaaggaga agaagttcaa acaattttag atgatgccga acgcagtatc ttggaggttt
    31561 cggaaaaaag aaatcgaagc ggctttttat ctattgctga agtactgaac tccacgattg
    31621 aaaatatcga tcgactagct caaaacaatg aagaaatcac aggtctacca actggctatc
    31681 aaggtctgga taaaatgaca gcaggtttcc agcccgaaga actaatcatt cttgctgcac
    31741 gtccagcggt aggtaaaaca gcgtttgcat tgaatattgc tcaaaatgta ggaacaaaaa
    31801 cagatcgttc tgttgcgata tttagtttgg aaatgggggc agaatctctt gtaaatcgga
    31861 tgctttgtgc agaaggttcg attgaggcca gccatttacg tacaggtcag ctttcggaag
    31921 aagaatggtc gaatctgatc gttgcgatgg ggagtctttc tcgagcaaat atttttatcg
    31981 atgatacacc agggatcaaa atctctgaaa tccgtgcgaa atgtcggaaa ctagcgcaag
    32041 aaaaaggcaa tctaggcttg atattgatcg attatttgca gttgattgaa ggtaacggta
    32101 aagagaaccg tcaacaagaa gtatcagata tctctcgcca actgaaaaaa ctggcgaaag
    32161 agctgaaagt acctgtcatt gctctttcac agttatctcg tggagtagaa cagcgacaag
    32221 acaaacgacc agtgctaagt gatattcgtg aatccggatc aattgaacaa gatgcggaca
    32281 tcgtagcctt cttgtatcga gatgattatt atgaaagaga aggcggagat gacgatgatg
    32341 gaaacgagcc aaaaaatgac aacgtcatcg aagtcatcat cgagaagaac cgaagcggtg
    32401 cacgaggaac agttgaacta ctgtttatca aagaatacaa taagttttcg tcgctttcgc
    32461 caagagaaga attttaaaga agaggttgtg agccttgagc aatcccgaga gctctcgtaa
    32521 gaagatgcaa ggagtaaccg caggaacgac tagagtactt aatttaaaca gatctccaaa
    32581 gggattgtga caaaagtctt gtcacagtcc ctttttccga ataaacggtg ttcaatcagc
    32641 tgacccttgg tattagctca gcaaacgtca aaatatgagg aactattttg acgtttgttt
    32701 ctctaatact caggtcaaaa cgctgatctt acgacctctt ttccttaaac ggtgttctcc
    32761 tgccaactcc tcggtcacaa gtcacaatat catcaaacat gagaagcagt tttttgaaat
    32821 tgctccttgt gactcagagt taaacggcag gctcacaacc tctttcttaa acggtgttca
    32881 aaagcttgca tctgcggtaa tacgcccaat caaacgaaaa atatgagaag ctatttttgt
    32941 ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctgat tcacggtgtt
    33001 ccaaaagtag cttcttcgaa aaataagtcg aaatttcaca aaaatttgag aagcaatttt
    33061 cgtacattcc ttcttatttc ttgaagctga ccacttctgt tacaacctct tttttaataa
    33121 ccattctaaa aagataaagt tcgtttttta agcgttttta taaaataaaa ataaaaatca
    33181 ttcgatttta tttgaaatat gtatctatta ttgatacaat ggttcaggat taaataatat
    33241 agaacgaggt gttcacatgt catctgtagt agtagtaggt acgcaatggg gcgatgaagg
    33301 gaaaggaaaa atcacagact ttctcagtga aaatgctgaa gtgattgcac ggtaccaagg
    33361 tggcgacaat gcagggcaca caattaagtt taacggtgtg acttataaac tgcatttgat
    33421 tccttctggg attttttata aagacaagat cagtgtgatc ggaaacggcg ttgtagtcaa
    33481 tcctaaatca ttggttaaag aattagctta tttacatgaa aaaggtgtag aaacaagtaa
    33541 tttgcgtatt tctgaccgcg cgcacgtaat cttgccttat catatcaaat tggaccaatt
    33601 acaagaagat gctaagggcg aaaataagat tggtacaacg attaaaggaa tcggacctgc
    33661 ctatatggat aaagcagctc gtgtaggtat ccgtattgca gacttattag ataaagaaat
    33721 ttttgaagaa cgtcttcgtt tgaatctaga tgacaaaaac cgtcaatttg taaaaatgtt
    33781 tgattcagaa ccacttgatt ttgacgatat ttttgaagaa tattatgaat acggacaaca
    33841 aatcaaacaa tacgtcacag atacttccgt tatcttgaat gatgctttgg atgcaggcaa
    33901 acgcgtgttg tttgaaggcg cacaaggtgt aatgctggat atcgaccagg gaacttatcc
    33961 ttttgttact tcttctaacc cagtagccgg tggggtaaca atcggaagcg gtgtagggcc
    34021 ttcaaaaatc aataaagttg tcggcgtctg caaagcctat acgtcacgtg tcggtgatgg
    34081 accatttcca actgaattat ttgatgaaac aggagaacaa atccgcgaag taggacgaga
    34141 atacggtacg acaactggtc gtccgcgtcg tgtaggctgg tttgattctg tagttatgcg
    34201 ccattctaaa cgagtttcag gtattaccaa cctatcttta aactcgatcg atgtattaag
    34261 tggattggaa acagtaaaaa tttgtacggc ttatgaatta gatggcgaac taatctacca
    34321 ctatccagca agcttgaaag agttgaaccg ctgcaaacca atctatgaag aattgccagg
    34381 ctggtcagaa gacattacag gatgcaaaac acttgcagaa ctgccagaaa acgcacgtaa
    34441 ctatgtacgt cgtatctcag agcttgttgg tgtacgtatc tcaacatttt ctgtcggacc
    34501 tgatagaaca caaacaaaca tcttagaaag cgtctgggcg caaatctaag gataaaaacg
    34561 acgactgaat caggacgttc ttaccagagc gagtcgaagt gtttatctat acgataataa
    34621 taacttgaac actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct
    34681 ttttccgaa
//
LOCUS       DACECJ010000020        34604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.020, whole genome shotgun sequence.
ACCESSION   DACECJ010000020 DACECJ010000000
VERSION     DACECJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.020"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(178..2070)
                     /locus_tag="IWO09_001050"
     CDS             complement(178..2070)
                     /locus_tag="IWO09_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263658.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="IWO09_001051"
     CDS             complement(2067..3794)
                     /locus_tag="IWO09_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263659.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="IWO09_001052"
     CDS             complement(3810..4460)
                     /locus_tag="IWO09_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0263660.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="IWO09_001053"
     CDS             4664..4960
                     /locus_tag="IWO09_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0263661.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5132..5752
                     /locus_tag="IWO09_001054"
     CDS             5132..5752
                     /locus_tag="IWO09_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0263662.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="IWO09_001055"
     CDS             complement(5844..7010)
                     /locus_tag="IWO09_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0263663.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="IWO09_001056"
     CDS             complement(7022..7375)
                     /locus_tag="IWO09_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0263664.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..8525
                     /locus_tag="IWO09_001057"
     CDS             7503..8525
                     /locus_tag="IWO09_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0263665.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8964..9398)
                     /locus_tag="IWO09_001058"
     CDS             complement(8964..9398)
                     /locus_tag="IWO09_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263666.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9508..11367)
                     /locus_tag="IWO09_001059"
     CDS             complement(9508..11367)
                     /locus_tag="IWO09_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0263667.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11485..12645)
                     /locus_tag="IWO09_001060"
     CDS             complement(11485..12645)
                     /locus_tag="IWO09_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0263668.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IWO09_001061"
     CDS             complement(12952..14376)
                     /gene="araA"
                     /locus_tag="IWO09_001061"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0263669.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14392..15090)
                     /locus_tag="IWO09_001062"
     CDS             complement(14392..15090)
                     /locus_tag="IWO09_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0263670.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15047..16708)
                     /locus_tag="IWO09_001063"
     CDS             complement(15047..16708)
                     /locus_tag="IWO09_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0263671.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16926..18326
                     /locus_tag="IWO09_001064"
     CDS             16926..18326
                     /locus_tag="IWO09_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0263672.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18578..19657)
                     /locus_tag="IWO09_001065"
     CDS             complement(18578..19657)
                     /locus_tag="IWO09_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0263673.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19743..20702)
                     /locus_tag="IWO09_001066"
     CDS             complement(19743..20702)
                     /locus_tag="IWO09_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0263674.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20714..22237)
                     /locus_tag="IWO09_001067"
     CDS             complement(20714..22237)
                     /locus_tag="IWO09_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0263675.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22247..22810)
                     /locus_tag="IWO09_001068"
     CDS             complement(22247..22810)
                     /locus_tag="IWO09_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0263676.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22816..23679)
                     /locus_tag="IWO09_001069"
     CDS             complement(22816..23679)
                     /locus_tag="IWO09_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0263677.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23693..24589)
                     /locus_tag="IWO09_001070"
     CDS             complement(23693..24589)
                     /locus_tag="IWO09_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0263678.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24764..26035)
                     /locus_tag="IWO09_001071"
     CDS             complement(24764..26035)
                     /locus_tag="IWO09_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0263679.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26025..27542)
                     /locus_tag="IWO09_001072"
     CDS             complement(26025..27542)
                     /locus_tag="IWO09_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0263680.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27740..28630
                     /locus_tag="IWO09_001073"
     CDS             27740..28630
                     /locus_tag="IWO09_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0263681.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IWO09_001074"
     CDS             complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="IWO09_001074"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0263682.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29539..31011)
                     /locus_tag="IWO09_001075"
     CDS             complement(29539..31011)
                     /locus_tag="IWO09_001075"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0263683.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31648..32010)
                     /locus_tag="IWO09_001076"
     CDS             complement(31648..32010)
                     /locus_tag="IWO09_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0263684.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32233..32985
                     /locus_tag="IWO09_001077"
     CDS             32233..32985
                     /locus_tag="IWO09_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0263685.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33125..34048)
                     /locus_tag="IWO09_001078"
     CDS             complement(33125..34048)
                     /locus_tag="IWO09_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0263686.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34063..34335)
                     /locus_tag="IWO09_001079"
     CDS             complement(34063..34335)
                     /locus_tag="IWO09_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263687.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10236 a         7199 c         5945 g        11224 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7801 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     7861 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     7921 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     7981 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8041 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8101 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8161 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8221 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8281 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8341 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8401 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8461 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8521 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8581 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8641 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8701 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8761 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     8821 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     8881 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     8941 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9001 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9061 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9121 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9181 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9241 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9301 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9361 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9421 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9481 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9541 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9601 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9661 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9721 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9781 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     9841 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     9901 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     9961 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10021 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10081 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10141 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10201 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10261 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10321 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10381 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10441 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10501 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10561 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10621 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10681 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10741 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10801 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    10861 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    10921 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    10981 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11041 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11101 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11161 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11221 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11281 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11341 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11401 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11461 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11521 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11581 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11641 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11701 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11761 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    11821 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    11881 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    11941 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12001 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12061 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12121 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12181 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12241 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12301 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12361 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12421 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12481 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12541 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12601 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12661 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12721 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12781 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    12841 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    12901 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    12961 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13021 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13081 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13141 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13201 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13261 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13321 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13381 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13441 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13501 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13561 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13621 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13681 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13741 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13801 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    13861 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    13921 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    13981 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14041 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14101 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14161 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14221 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14281 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14341 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14401 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14461 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14521 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14581 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14641 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14701 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14761 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    14821 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    14881 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    14941 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15001 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15061 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15121 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15181 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15241 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15301 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15361 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15421 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15481 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15541 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15601 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15661 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15721 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15781 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    15841 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
    15901 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    15961 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16021 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16081 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16141 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16201 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16261 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16321 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16381 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16441 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16501 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16561 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16621 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16681 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16741 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16801 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    16861 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    16921 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    16981 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17041 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17101 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17161 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17221 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17281 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17341 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17401 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17461 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17521 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17581 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17641 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17701 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17761 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    17821 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    17881 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
    17941 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18001 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
    18061 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18121 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18181 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18241 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18301 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18361 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18421 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18481 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18541 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18601 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18661 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18721 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18781 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    18841 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    18901 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    18961 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19021 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19081 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19141 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19201 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19261 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19321 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19381 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19441 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19501 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19561 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19621 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19681 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19741 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19801 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    19861 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    19921 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    19981 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20041 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20101 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20161 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20221 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20281 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20341 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20401 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20461 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20521 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20581 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20641 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20701 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20761 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    20821 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    20881 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    20941 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21001 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21061 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21121 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21181 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21241 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21301 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21361 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21421 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21481 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21541 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21601 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21661 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21721 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21781 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    21841 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    21901 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    21961 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22021 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22081 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22141 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22201 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22261 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22321 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22381 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22441 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22501 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22561 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22621 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22681 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22741 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    22801 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    22861 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    22921 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    22981 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23041 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23101 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23161 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23221 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23281 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23341 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23401 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23461 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23521 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23581 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23641 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23701 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23761 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    23821 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    23881 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    23941 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24001 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24061 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24121 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24181 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24241 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24301 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24361 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24421 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24481 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24541 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24601 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24661 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24721 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24781 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    24841 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    24901 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    24961 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25021 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25081 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25141 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25201 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25261 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25321 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25381 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25441 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25501 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25561 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25621 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25681 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25741 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25801 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    25861 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    25921 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    25981 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26041 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26101 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26161 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26221 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26281 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26341 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26401 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26461 tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca agcaatcttt
    26521 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26581 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26641 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26701 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26761 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    26821 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    26881 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    26941 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27001 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27061 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27121 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27181 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27241 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27301 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27361 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27421 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27481 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27541 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    27601 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    27661 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    27721 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    27781 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    27841 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    27901 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    27961 ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac
    28021 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    28081 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    28141 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    28201 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    28261 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    28321 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    28381 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    28441 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    28501 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    28561 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    28621 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    28681 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    28741 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    28801 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    28861 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    28921 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    28981 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    29041 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    29101 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    29161 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    29221 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    29281 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    29341 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    29401 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    29461 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    29521 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    29581 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    29641 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    29701 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    29761 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    29821 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    29881 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    29941 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    30001 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    30061 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    30121 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    30181 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    30241 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    30301 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    30361 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    30421 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    30481 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    30541 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    30601 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    30661 cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    30721 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    30781 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    30841 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    30901 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    30961 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    31021 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    31081 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    31141 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    31201 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    31261 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    31321 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    31381 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    31441 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    31501 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    31561 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
    31621 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
    31681 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
    31741 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
    31801 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
    31861 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
    31921 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
    31981 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
    32041 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
    32101 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcatgaga
    32161 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
    32221 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
    32281 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
    32341 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
    32401 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
    32461 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
    32521 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
    32581 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
    32641 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
    32701 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
    32761 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
    32821 cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca taaaggacta
    32881 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
    32941 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
    33001 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
    33061 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
    33121 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
    33181 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
    33241 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
    33301 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
    33361 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
    33421 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
    33481 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
    33541 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
    33601 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
    33661 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
    33721 cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt gtcactttgt
    33781 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
    33841 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
    33901 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
    33961 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
    34021 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
    34081 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
    34141 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
    34201 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
    34261 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
    34321 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
    34381 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
    34441 aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc tttattatta
    34501 gtgaatggat atttactttt aatagataaa atattataac tattaaaaat aactattttc
    34561 attataatta cacacaaaaa agagcgtgag acaaaagtaa agaa
//
LOCUS       DACECJ010000021        34023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.021, whole genome shotgun sequence.
ACCESSION   DACECJ010000021 DACECJ010000000
VERSION     DACECJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.021"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(524..1078)
                     /locus_tag="IWO09_001080"
     CDS             complement(524..1078)
                     /locus_tag="IWO09_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0263688.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1204..3132)
                     /locus_tag="IWO09_001081"
     CDS             complement(1204..3132)
                     /locus_tag="IWO09_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0263689.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3438..4154)
                     /locus_tag="IWO09_001082"
     CDS             complement(3438..4154)
                     /locus_tag="IWO09_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0263690.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4155..5978)
                     /gene="glpO"
                     /locus_tag="IWO09_001083"
     CDS             complement(4155..5978)
                     /gene="glpO"
                     /locus_tag="IWO09_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0263691.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5992..7488)
                     /gene="glpK"
                     /locus_tag="IWO09_001084"
     CDS             complement(5992..7488)
                     /gene="glpK"
                     /locus_tag="IWO09_001084"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0263692.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7624..9084)
                     /locus_tag="IWO09_001085"
     CDS             complement(7624..9084)
                     /locus_tag="IWO09_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0263693.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9074..10369)
                     /locus_tag="IWO09_001086"
     CDS             complement(9074..10369)
                     /locus_tag="IWO09_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0263694.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10701..11378)
                     /locus_tag="IWO09_001087"
     CDS             complement(10701..11378)
                     /locus_tag="IWO09_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0263695.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11408..12499)
                     /locus_tag="IWO09_001088"
     CDS             complement(11408..12499)
                     /locus_tag="IWO09_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0263696.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12600..13034)
                     /locus_tag="IWO09_001089"
     CDS             complement(12600..13034)
                     /locus_tag="IWO09_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0263697.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13618..15075)
                     /gene="ascB"
                     /locus_tag="IWO09_001090"
     CDS             complement(13618..15075)
                     /gene="ascB"
                     /locus_tag="IWO09_001090"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0263698.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15072..16967)
                     /locus_tag="IWO09_001091"
     CDS             complement(15072..16967)
                     /locus_tag="IWO09_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0263699.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17025..18800)
                     /locus_tag="IWO09_001092"
     CDS             complement(17025..18800)
                     /locus_tag="IWO09_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0263700.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18850..20346)
                     /locus_tag="IWO09_001093"
     CDS             complement(18850..20346)
                     /locus_tag="IWO09_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0263701.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20540..22162)
                     /locus_tag="IWO09_001094"
     CDS             complement(20540..22162)
                     /locus_tag="IWO09_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0263702.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22611..23924)
                     /locus_tag="IWO09_001095"
     CDS             complement(22611..23924)
                     /locus_tag="IWO09_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0263703.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24169..24393)
                     /locus_tag="IWO09_001096"
     CDS             complement(24169..24393)
                     /locus_tag="IWO09_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263704.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24413..24781)
                     /locus_tag="IWO09_001097"
     CDS             complement(24413..24781)
                     /locus_tag="IWO09_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0263705.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24979..25689)
                     /locus_tag="IWO09_001098"
     CDS             complement(24979..25689)
                     /locus_tag="IWO09_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263706.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25707..26204)
                     /locus_tag="IWO09_001099"
     CDS             complement(25707..26204)
                     /locus_tag="IWO09_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0263707.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(26241..26693)
                     /locus_tag="IWO09_001100"
     CDS             complement(26241..26693)
                     /locus_tag="IWO09_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0263708.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26828..27433)
                     /locus_tag="IWO09_001101"
     CDS             complement(26828..27433)
                     /locus_tag="IWO09_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0263709.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27883..28503)
                     /locus_tag="IWO09_001102"
     CDS             complement(27883..28503)
                     /locus_tag="IWO09_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0263710.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28505..29374)
                     /locus_tag="IWO09_001103"
     CDS             complement(28505..29374)
                     /locus_tag="IWO09_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0263711.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29397..29804)
                     /locus_tag="IWO09_001104"
     CDS             complement(29397..29804)
                     /locus_tag="IWO09_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0263712.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29823..31112)
                     /locus_tag="IWO09_001105"
     CDS             complement(29823..31112)
                     /locus_tag="IWO09_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0263713.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(31139..31441)
                     /locus_tag="IWO09_001106"
     CDS             complement(31139..31441)
                     /locus_tag="IWO09_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0263714.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31466..33559)
                     /locus_tag="IWO09_001107"
     CDS             complement(31466..33559)
                     /locus_tag="IWO09_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0263715.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
BASE COUNT         9659 a         7313 c         5652 g        11399 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
       61 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
      121 aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
      181 ggatttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg
      241 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa caaacggaaa
      301 aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
      361 cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa
      421 aacaacttct cctgtttttg gtttggttgg cttattatcg agaagtagct actgggagtc
      481 tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt ctttgaaaca
      541 tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg
      601 caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa aggatcatgc
      661 caacatagat gacagcagtc gctactttgc cataccactt cgctccactt aatttgatat
      721 ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc aagaatagac
      781 caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga aaaagttgtg
      841 tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga ttatatctcc
      901 gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc gctcctgcta
      961 tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa atacggaata
     1021 tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt aatttcattt
     1081 tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg ggaataaaac
     1141 caatgatttc actgttttta tttttcttta cagccactga tattatgagc taatttgaga
     1201 taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc gcatttcttt
     1261 tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt
     1321 tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat cttctgctga
     1381 acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta aatatacatc
     1441 aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt cattgacaaa
     1501 tatccgatca ccttttgcaa agagctggcg ttctttttta ttctccttcc tatttgtaac
     1561 tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg ccaagtaacg
     1621 ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc acaaagcttg
     1681 gctttctcct gtttctccaa cccgcaactg atatttagga cgaagtgctt cccgatcgaa
     1741 agccatagca gccggcacaa aatcttcatg atctccggcg aatttcttga tttctccata
     1801 atgggtcgtg gcaatgatca atgccccttg ctcatacata gattccatga ttgcgatcgc
     1861 tagtgcagcc ccttcatttg gctctgttcc actgccaatc tcatctaaaa gtaccaacgt
     1921 attccttttt atctttttca aaatagcagc gatgttttgc atatgaccag aaaatgtact
     1981 caacgcattt tctaaatttt ggtggtcacc gacatcgacg aaaatctcat caaagacagc
     2041 aagctctgtc ccttcttttg ccggaacttg cataccgaac atcgccatca aagtcaataa
     2101 accgactgtc ttcaacacga ctgttttgcc tccggcattt gctccggtga taatcaagcc
     2161 tcgatagtct ttaccgattt caacatccaa tggtaccgca tgctcaggta aaaatggatg
     2221 tctgccttgc ttgataatga tatgttccga tttattgatt ctaggtgtgc ttccattgat
     2281 ttctcggcta aattttgccc gagcaaagat gatatctaac accgtgatcg tttcgatcaa
     2341 taaatcgatc acttcttcgt tttctgcgat agcaccagtc aatgccgcca ggacttgata
     2401 ttcttcactg atttcttcag cttttaacaa ctgatagtgt tcattcaatt tagaaatcac
     2461 agttggttca ataaaaaccg tcgttctttt atttgattca tcgataatcg ttccatctat
     2521 tttattttta tagctggctt tgataggaat cgtatagtat tcgccttttt gaacaatcat
     2581 tgcttcttgg atcatttctt tgtttttcgg atgacgcaag aatttcagta attttgactg
     2641 gatttctttt tcgattagtt gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc
     2701 gtcactgact ttattatttt ttattttctg gtcaatcaac tcttctacat ttatcaaatc
     2761 aggaagatgt ttgctataag caaacaacaa gggtgtctgg tattgattct tatcaaaaaa
     2821 ctttgtgatc atccgactgc ttctaagaaa atccgcatat tcgatcaaat cggttggctg
     2881 cagaatcaac ccttttttga cttgttctgt cagtgcatcg atcctcggca accccataaa
     2941 tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc
     3001 caagacagtt tgtaggtttg tcgagaccgt catatcgctg attcgttttt tgctatagtt
     3061 tccaattgct cttgcttgga tttccttttt gatccgatca aattgtaatt gactaatcat
     3121 ttcgttgtcc atattctctc cttttcttga agagatacct gtcccaaaat aaataagggc
     3181 ctagagataa ataaaaggat agaacacagc ttaaaaaagt catgtcctat ccttattaga
     3241 atacaaagat attgttttcc tgacaggtac cgtttcataa gcacaccaaa taaactttgt
     3301 ttattggcta ttcgacttac tgattagata ccagctacac tcacaaaaca taccccattc
     3361 ttctaggtct tatttatttt ccacttaact atagcaagac acattttggt tgtcaaactt
     3421 tagtatgttt tatagattta caccattaat gaatagacac ctaccgcgat aattgctccg
     3481 atcattggac ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc tttattagct
     3541 attggcaata ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt gattgcatat
     3601 cctgttggcc ctcctaatga aagaccgatg gctaagatca atccacctac agcgaatacg
     3661 ttcgttccag gtgcaaattc gttttgtcca aaagctagca acccaagaac taatacaaat
     3721 gtaccaatta attctgtgat cacatttgct ggataattcc gtatagcagg acctgttgca
     3781 aacgttccta aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa ataagaaagc
     3841 caaacaagaa tggcccctgc aaaagctcct aaaacttgcg caatgatgaa aggaacgaca
     3901 agattccaac taattgcccc tgtcatcgcc atagctagag agactgcagg attcaaatga
     3961 gctgggccca tgaatccaga aatatagaca gccattgtta cagcgagtcc ccaaccaaat
     4021 gcgatgacaa tccagccaga agcctgtgct ttacttttgt ttaaattcac agccgcacac
     4081 acaccatctc caagcaaaac taggatcagt gtcccaataa actctcccat gatttgagtc
     4141 atatcactgt tcatttattt ctcccccttt agttcccgca gatctgattc tgcaatcaat
     4201 gcttctaaac gtttagtctg ttgcgccttc tcctcttctg tccattcaaa atatccagca
     4261 attgcatcga tcacaggttg cttgatctca tcaagctgat ctcgttcaaa caacagatga
     4321 tttgtacgac gaatgagata atcgcctggg gccaaaacca tttcttcttc taaaccataa
     4381 cgtaaccgag ctgactcagc gagactcaaa ccaggataag gtgccatttc ttttgctaat
     4441 tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga tataagtagc atcttcttcc
     4501 tctaaaccgg ctgctactcc ttcttttgct aattctgtca ctgtttcttc taatttagtt
     4561 ggatcgaagt ttccacctga aatctgatat tttttagagt cgatttcttt cgtctctatt
     4621 ccgtattctt cttttaataa ttggcgtatc aatcgcaaag ctccttctgc catcttacgg
     4681 taatcggtga ttttcccacc agataaagtc agcaaaccat ctggttctct ttctaaagaa
     4741 ctacctctgg aaatcgtaga aggtgctttt tcatcacggc tgttttccaa atggttcaac
     4801 acgtcttcta cttcagcacg agaaactttg ttttccttat attcacttac tgtatcgaca
     4861 actttattga agctcttgtc tgaaatcgat ccattatctc caccattgta atcagaacca
     4921 gaattaccaa tcaacagtgg acgaagtcct gcccagcttg cttcgatatc attcaatgtg
     4981 atatttgctt ctggatagcg atgattgatc acatccaata gataatctac atcttcttgt
     5041 gtgactttcg gatccgtaaa atctccttgg taatccgtat ctgtcgtacc aaagtaagtc
     5101 ttgttttctc ttggaatagc aaaaaccatc cgcccatctt gctttcctgt atcgaagtat
     5161 gtgggttgcg gtacaggcag ttttttcgca tctacaacta aatggatccc tttagttgga
     5221 cgcattttag gagagactgg acgcgtaaaa ttcaagtttc gtactttatc tacccaagga
     5281 ccactcgtat tgatcactaa ttttgctttg atttcgatca cttcatctgt cagcaggtca
     5341 cgagctttga cgccaacgat ttgatcgcca tcatataaaa agccaaccgc tttcatttta
     5401 cttactagat aagccccatc ttcggcagcc tttttgatat tatcaatcac taaacgggca
     5461 tcattgttgc ggaaatctag atagacaccc gcgcctttta gcccttcttt tttcaaaaac
     5521 ggttctcttt ccaatacttc ttctggtgtc agcgtataat tctcatattt ggttcctgtc
     5581 acatttgcca gtttgtcata aaggtccatt gctactttga ccgagaacat attgaaggtt
     5641 gttgctcctt cgccctcata gattggcaaa agcattggat ctggttttgg gatgtgtggg
     5701 gcaatacttt gtacgactgc acgttcacca actgtgtcag ctactacttc cacatcaaat
     5761 gtcttcagat aacgaatacc gccatgaaca agtttggtcg agcgagagga agtcccttct
     5821 gcaaaatcct gcatttcgat caatcctgtt ttgattcctg atgctgctga ctggactgcc
     5881 acaccggcac cagtgattcc gccaccgatg atcaacagat ccaattcttc ttgctgcatt
     5941 ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat ggatttcctc cttattcaaa
     6001 tgcttgggta gcttttactg cttttttcca accgttgtaa agtttttctc ggcgctcttc
     6061 ctccatttga acttcaaata gcttgccagc ttcataaaat tcgcggatct cgtctgtatc
     6121 tttccaataa ccgacagcta atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa
     6181 gttttctgct cgttggatcg gtacattcaa gatatctgtt tggaattgca tcagatattc
     6241 attgtttgct gcaccgccat ctactttcaa tacaggaatc ttgattccag tgtcatcttg
     6301 catcgtatcg atgatatctc tcacttgata agcaatcgac tgtaaagtag ctttaatgat
     6361 atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca
     6421 ataaggagca cctagcccaa cgaatgcagg aacaacataa acctcatctt cgcttgtaga
     6481 gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac atttgaagtc catctctcag
     6541 ccattgaatc gaagaaccag ccacaaaaat acttccttct aaagcatagt aaacctttcc
     6601 atttatgccg taaccaattg tcgtcaataa attatttttg gataattgcg gtttttcacc
     6661 ggtattcatg acaataaatg aacctgtacc ataagtattc ttgaccatgc ctggttcgaa
     6721 tgccatttgt ccgaataatg cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc
     6781 gcttccataa aaatgataac cttgcgtata accataaact tctgagttag aagtgacttt
     6841 tggtaacatc actcgaggaa tatttagtaa atctaaaatc tcctgatccc aatctagctg
     6901 atggatattg aacaacatcg tccgactggc attcgagtaa tcagtaacat gtgcttgtcc
     6961 atcggtcagt ttccatacta accaagaatc gatcgttcca aacatcaatt cgcctttttc
     7021 agctcgttct tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg ttgctgagaa
     7081 ataggcatcg atcactaacc ctgttttttc gtggaaaaat tcttgatagc cttcattttt
     7141 taagttatct gcaattccag ctgactgtct ggattgccaa acaactgcat gatagattgg
     7201 acgtcctgta tctttttccc atattactgt tgtttcacgc tggttcgtta tgccaatccc
     7261 agcaatctga tttggtttga tccctgattc gatgaacgct ccggcaataa ctgactgtac
     7321 agaattccag atttcatttg catcatgttc cacccaactt tcttgcggga aatattgcgt
     7381 gaactctttt tgagaactgc cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga
     7441 gctagttgta ccttggtcaa tcgccatgat atacgttgat tctgtcattg tgattccctc
     7501 cattatatgc aaacgctttc tttaactact cttatcatac tactgtttta aaaaaagaaa
     7561 ctgtcaaaat tttattcttt ttttcttttt ttgacagttt tttattatga agctattttt
     7621 taatcaattc ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta
     7681 aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct ggcaaaatgt
     7741 gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac cgccgattcc
     7801 cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca gactttctaa
     7861 atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa gaacattggc
     7921 atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg gcaattgctg
     7981 caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga gcattttctg
     8041 ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa agaaaaacca
     8101 ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga ttttcaaacg
     8161 acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg
     8221 taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa
     8281 agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg ctgctgttcg
     8341 actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg actgatagag
     8401 taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc tttttttcgt
     8461 aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac
     8521 acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg
     8581 ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct gtagctcttc
     8641 tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct ggttcaattc
     8701 tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta atcgaacgat
     8761 ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc tttcctgcaa
     8821 taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca gcgcctgata
     8881 ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt caatatcttc
     8941 caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac cattgataga
     9001 ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact gctcttttct
     9061 ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac accatatctg
     9121 gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca aacgtccatc
     9181 cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt ttgctttgga
     9241 gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta actttgcttt
     9301 gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg ggataaaaaa
     9361 tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac tgattgccga
     9421 ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg ctgacatttc
     9481 gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt gttggacgaa
     9541 aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt
     9601 catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac atctgattgt
     9661 cggggcgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt ttttccgtct
     9721 ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa
     9781 gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct agataacgaa
     9841 aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat gccaaccctt
     9901 cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt gcattcatga
     9961 tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata agcgttgatt
    10021 ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat
    10081 ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt actgtctttt
    10141 ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc caagaaagtt
    10201 gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga atccatccta
    10261 acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg cgaagataaa
    10321 tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc tgcattcctc
    10381 tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat tcataactcg
    10441 ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc ataccgtgtt
    10501 ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca tttctgaaga
    10561 gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg tacctctgtc
    10621 caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg taatagttag
    10681 aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc gcctaattga
    10741 tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac cacttctgaa
    10801 aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc tgctaagaat
    10861 ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc agtcactgta
    10921 cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc tacaacaccg
    10981 tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac tttcccaaga
    11041 tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata aaccgctgca
    11101 taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg atttctgaaa
    11161 tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact ttgcggttct
    11221 aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt ttttactaat
    11281 acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc gatcttttcg
    11341 tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc tttctataaa
    11401 aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc aaataaagct
    11461 ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc ctcacggttt tccaataatt
    11521 tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt tctgcatatt
    11581 tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact aaagggatct
    11641 ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat ttatcagaac
    11701 gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt gactcacgta
    11761 atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca tattcttctg
    11821 gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc gctgagaaaa
    11881 ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg ccccattgat
    11941 cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta tttgctccat
    12001 gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg ccaaatgctt
    12061 ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt
    12121 ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct cgacttgtca
    12181 ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg attgctttcg
    12241 ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca cctggccagc
    12301 cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga cctttagaac
    12361 gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg
    12421 tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt aaagtaattg
    12481 attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa cctatcgaga
    12541 atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct taaaaaattt
    12601 tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc tttacttgct
    12661 tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc atcgacttcc
    12721 ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc ttcgctaact
    12781 gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg
    12841 gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt taatatcaat
    12901 aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc agctaattta
    12961 ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa cgctttttca
    13021 attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga tttttatttt
    13081 ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa aaagaagtag
    13141 ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca ccgtgaatca
    13201 gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga aaatggcttt
    13261 ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac accgtttata
    13321 tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa caaaaatagt
    13381 tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt acaccgttta
    13441 ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt caaaaaacag
    13501 cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga gaatactgtt
    13561 tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt ccttttatta
    13621 taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa
    13681 ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc cataacgttt
    13741 tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg tataaccaaa
    13801 taaatctact ccatctttga tagcttcctt catctgctca atatgctgtt tcaaatagtc
    13861 aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag ctccgagtcc
    13921 gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca aagcaattct
    13981 caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg gatttttaat
    14041 tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag cactgactgc
    14101 actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta agatttcttc
    14161 atcacctact tctattttta agttgacacc tttttcttca aacattcgtt gcgtatacgc
    14221 tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt gctgctcaat
    14281 tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat agctgatcat
    14341 acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta aagcacttgc
    14401 tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat ggttttcttc
    14461 aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat tgaaagtcat
    14521 ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa
    14581 ctgaataagc tttcgattag gccatccacc atattctgta accaaatata acggcatttc
    14641 ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt caaacacttg
    14701 atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag gaaaaattct
    14761 tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata aacggatgtc
    14821 ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat acttgctgtt
    14881 gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat ctgtccattt
    14941 ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc ctttttcatc
    15001 tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata aaaatccttc
    15061 tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg attatttttc
    15121 ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac cattgtggtc
    15181 ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa atcgccttta
    15241 cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa attcactgta
    15301 tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc aattgcatgt
    15361 ttcgttggaa aaactgttga aacttccccg tttacaggac tgtagatttt tccgtccgtt
    15421 ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc gtttacttca
    15481 gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt tgaggattct
    15541 tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact gatcatcgct
    15601 aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa
    15661 atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac tccaccaaat
    15721 atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt cacaccataa
    15781 acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga taaacttttt
    15841 actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg cgcagctacg
    15901 gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt gttggtcatt
    15961 actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa tccgccgata
    16021 acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc tgcaatcagc
    16081 tgacttgcat aatctgcaat tggacctaca actaataaag taattgggac catcacgatg
    16141 atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt gcgaatctta
    16201 ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt acttgtatac
    16261 cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact atatgcactg
    16321 acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg atctgtattg
    16381 aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct
    16441 gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa actcgtacat
    16501 aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt cggggcaaaa
    16561 acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt tcctctcttt
    16621 ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata cacttcattt
    16681 actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt gatcacacct
    16741 tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt aaagcgtaac
    16801 cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa tcggacaata
    16861 tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa tgccgtagca
    16921 atccttctta caagataagt atagttctta gaaaacggtt tctccattca attagtaccc
    16981 cttataatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc gagcaaatca
    17041 ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga gacaaaaaaa
    17101 acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata ctctgtgatt
    17161 tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt taacggaagc
    17221 ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt tttatggatt
    17281 tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt tttttcttca
    17341 actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa ctcatctaga
    17401 acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc agtagtcaaa
    17461 attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac tggttctatc
    17521 cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag tgtattcaaa
    17581 atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa aggattcaac
    17641 gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg taattgaaaa
    17701 tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga gatgatcttt
    17761 tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat ttgtttgata
    17821 tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc taaaaaacga
    17881 ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt ttcatcatta
    17941 atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc ttgattggaa
    18001 aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat cggaataaat
    18061 aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt caaaacgaga
    18121 taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga tataagaacc
    18181 tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga
    18241 ggcaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt
    18301 gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta
    18361 ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa
    18421 agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac
    18481 gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct
    18541 tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta
    18601 tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg
    18661 atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc
    18721 ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac
    18781 ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag
    18841 gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag actggataat
    18901 ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt
    18961 ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac
    19021 gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc
    19081 agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt
    19141 caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc
    19201 ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact
    19261 ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt
    19321 atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat
    19381 ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca
    19441 gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc
    19501 ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca
    19561 gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg
    19621 atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc
    19681 ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa
    19741 tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt
    19801 catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa
    19861 aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt
    19921 ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag
    19981 tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat
    20041 tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat
    20101 atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc
    20161 gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt
    20221 catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc
    20281 ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg
    20341 atccataact atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat
    20401 caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt
    20461 catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt
    20521 aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat
    20581 caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt
    20641 cacgtggaac agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct
    20701 ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat
    20761 agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat
    20821 aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat
    20881 tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca
    20941 taggcgtcct agcattgtct cttcctttcg cattgattga ttccaagatt tcctcttttg
    21001 taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg
    21061 cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg
    21121 tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt cgatacacat
    21181 cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat
    21241 tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga
    21301 tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt
    21361 cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt
    21421 cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt
    21481 gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg
    21541 gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc
    21601 agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag
    21661 gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag
    21721 cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc
    21781 aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat
    21841 gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt cttttctttg
    21901 cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat
    21961 aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa
    22021 ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc
    22081 caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat
    22141 ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct
    22201 taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac
    22261 cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt
    22321 ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg
    22381 ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatatgagg aactattttt
    22441 tcgttttttt ctctaatact caggccagaa cactgatttc acaacctctt tataaacggt
    22501 gttcactcag ctgtccctcg gtattagctc aaaaaacggc aaaatataag aaactatttt
    22561 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
    22621 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
    22681 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
    22741 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
    22801 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
    22861 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
    22921 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
    22981 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
    23041 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
    23101 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
    23161 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
    23221 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
    23281 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ctatcccgat gaagatgcca
    23341 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
    23401 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    23461 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    23521 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    23581 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    23641 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    23701 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    23761 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    23821 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    23881 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    23941 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    24001 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    24061 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    24121 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    24181 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    24241 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    24301 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    24361 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    24421 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    24481 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    24541 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    24601 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    24661 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    24721 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    24781 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    24841 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    24901 aggattgctt tcctccacaa acttttttta caaaaaaaaa aaacggcaat gaatgccgct
    24961 cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta gtgtgtgtct
    25021 aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga ttgcctgtgc
    25081 gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg cttgtgtttc
    25141 ttccgtcagc attgttcttt gggcttcacg ccagttgaga cagcgacaga tcgctccttg
    25201 ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg cacctaaagg
    25261 caggaatgat tcgccacctt gggcttttcc taaatgcaga tttcctgcga tcttttcgat
    25321 atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga tatctacaag
    25381 cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag cttcaataga
    25441 agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact gagcaatgac
    25501 ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg ctgcctcatt
    25561 caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga taccatttac
    25621 ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat ccactattac
    25681 ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag atagtctgtt
    25741 tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc ttttttacga
    25801 taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc tgttttttgc
    25861 ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc ttgatgttta
    25921 aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat ttcctcagat
    25981 tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat actcactagg
    26041 aaaaaatcgg tttctttatc ttttagctct tctgtaagtt tttctagtgc ataagcttgt
    26101 gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc caaatgtatc tgtaaatgtt
    26161 tcgatactaa tttgttgtag taagtctaca tctgaaggcg tcatttttcg aatttcgtat
    26221 ttattcacgg cttcttcctt tcaatagatc cttttattgc cttttttgac aaaattccaa
    26281 tcttcgctaa cattcttgca tactttattt aaaagatatt ccaattgttt tgcttcttcg
    26341 tcagaaagac catttaatgc gactgcattg gagtagttgt tttctcgaat gatgaaagga
    26401 tagatttctg ctcctttttt agtcggatag agtttcttga tttttttatt ttctttatct
    26461 tccaaacgtt cgatcatccc attggattct aatttcttga ttgctcgggc agtcgttgta
    26521 cgatctactt tgatcatttc tgctagtttt tcttggatga ttccaggatt ttcgcaaata
    26581 cgtactaaat aaagatactg tcctcttgtt aaatcgactt ctttgaattc gatattgcta
    26641 atagaatcta gcgctcgggc aatcattccg atgtctcgaa ggatttcagc catttcatcc
    26701 cacctttatt ttgtttgtaa gactagttta cctctttttg ttgcaaatgc aactattttc
    26761 aataaaaaaa tcaagttctt ttttaataac tagtaaagta tattcttttc ttcttttacg
    26821 aaaaagctca atcacaatag atctggtttt tgttttctaa aataagtaac tgtttcttcg
    26881 ttgttcgctt ttctgatagc tttggcaact cttctatact aaaaaatccc atgttggaag
    26941 tttcattatt ctcaataaac ttggcctctt caccgtggat cgcacaagca aaaacaagtt
    27001 tataatactg aaaaagctgt ggaatatctt tttgtttatt cgtatcaaaa actgctcgca
    27061 actgagtagt ttctactgtt aatcctgttt cttcatagac ttctttacgc acattttctt
    27121 caggagacaa tcctatttca gcaaatccac caggaagtga ccattctttc gtccgtttat
    27181 cttcgattag taggaccttg ccatcttttt tgatataggc acgaacatct acttttggtg
    27241 ttggatgtcc ttctccaatc gttgtgagcg cttttatttc ttcgaaagtt tcttttccta
    27301 tttcactgat cagttccaat gaaatcgtcc ggatctcctc aaaacgctct ttatcaaata
    27361 catcttttcc ataatataga ccagcttgag aaagtgcgag tagtcttttg tattgagcca
    27421 acttcttgtt cattctcttc ccttcttttt tagcaaatgt atgactatgt aatcaagtat
    27481 agaagactgg tttaattacg gcaaataaat ataatgaaat ctgcaataaa taaaagaatg
    27541 ttaaaaaact cgagaacaaa agacattcga cttctctggg gctgtgaata aatggcgttc
    27601 aaaagcttgc atctgcggta ataagcccca ccaaacgaaa aatatgacga gctattcttg
    27661 tctggctgtt cttattaatt gaggcaggac agctttttca caacctcccc actaaacggt
    27721 gttctcctac caactcctcg gtcacaagtc acaatattca tcaaacatga gaagctgttt
    27781 ttttgaaatt gctccttgtg actcggagct gaacgacagg ctcacgacct ctcggtaaac
    27841 agcgttcaaa agccaatttt tcgatattaa gccatttctt tgttacttat tgtcattgat
    27901 ttctttttct aatttatcca atagtggagt gtaatccact tgagtatagt ctgttacatc
    27961 aaattcatat agtgttccta actggaattt gtcatatttt cggtcggcga tgatcttttt
    28021 gaatgcttct ttcgtaatat gattggtccc tttcgttcga tcttccaata cgtcaccata
    28081 gtgataatga tccatgatat agctcaaatg acgatctgat tcacgatcac gtttgtaccg
    28141 acgttgacat aacacttcaa agtctgctgt taggcgaatc gtgatcacct gatatccgta
    28201 tctatctgct aattcttgga gtcgtgcttt ttgcttatca ctaaaaggat actcggacac
    28261 aatcactttc tttcctgctt ccatatacaa atcaagtgct ttgtaataga aaggataaac
    28321 tttcgtcctt tcttgttccg cccgttcttc caaattgtta aacccgatgg attctgcata
    28381 gtattccttg atttcatcga gcgtgatggc atacatatct ggaaatcgtt cccgtagttt
    28441 attcataaga tatgtttttc ctgtaccagg actaccagcc agaagaatga aatacttttt
    28501 catctcattt atttcctaag ttaattggac tttccaagtt cataaacagt ccaatcttat
    28561 gcttgacaat ttcttttact gcttcatttg ctggaatcac attatggcga agagacttgt
    28621 tgatttctgt ttcactaaag gtttctttag ctactttcgt ccagttgact aaaagctccg
    28681 ttccaacatt gaatttgcgg atcccgttat tgatcaattc aggataatct tcttctttca
    28741 cacctgttcc tccatggata actaggggga catcaactac tgcatcgatt tctttcagca
    28801 agggaatgtt tacttctgtt tttgatttga attgtccatg attcgtaccg atagcaatag
    28861 ctaatgcatc cacgcctgta gcttctacaa atttcactgc gtcttccggt cttgtatata
    28921 ccttgtcatt ttcagcgaca tggattcctt cttctgtacc gccgattgtt ccaagttctc
    28981 cttctacgga aacaccgtgc gcatgggcat attctactac tgcttttgtc ttcaggatat
    29041 tttctttgaa aggcagatgg gacccatcat acatgacaga agtatatccg ctgtcgattg
    29101 cttctttgat atcatcaaag tctcgggcgt ggtctaagtg caagactgca tcaaccattt
    29161 cttcttctgc aatagctgtc attacattga ctaacacatg catgccgata tatttagcgg
    29221 tatcgacact tgtttgaata atgatcggcg aacccatttc ttttgctgcg cctagcatag
    29281 caggaagcat ttctaggtta tgcgtattaa aagctccaac tgtaaaattc aactcttctg
    29341 ctgtttttgt tacttctttc aatgttgtat acatctttta ttgccacctt atctttttat
    29401 ttgtttcctt tgatcgttac ttgttttgca atttgtcgca tctcatccat tttgcccatg
    29461 tagtaatcga tcccttgatc atcaccagca cgagcagcat ctgcacgctt ctggttatag
    29521 aggctcatca gttttttata cccatctccg atcgtctgtt tattttccaa actcttttct
    29581 aaatattgga tggcttgatc gttcacatac aattcagaaa atttaagtcc aacttgttca
    29641 taaagtttcg ctaatttatg cttatcttgg gtagtggaaa tctcttgttt caattgtgta
    29701 atctctgatt gaatcttttc ggtcgcttct ggcgataggg ctttttcttc ttttgccaga
    29761 acttttttcg gttctttttc ttttttctta aaaaatccaa acatcatttt ccctactttc
    29821 aattaaaatg ctttcaaaac tgggcttaag atccaggcat ataagagtgc tgcggctact
    29881 gtatctacat cggttgctgt agcattgatg aatcccatcg aattaaagat cgttactagc
    29941 aaagcgggca ataatgtaat aaagaaacca tgagcaatac cgccaatgat cgctcctcgg
    30001 cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc cggcaaagaa attggttagc
    30061 ataccaggta aaatcatcgc taagccgaac atgggcaaag taaacatggc aataatcgta
    30121 ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg ggctataagg aaagaaaact
    30181 ggacaatcta aggcagggat cgcatcagga actaatttca tagctatccc tcgaaatgcc
    30241 ggcacgattt cacctaacag tagacgtaca ccagctagca atacatacac acccactacg
    30301 aattggatgg cttgtaaaaa ggcaaacatg atatagtttt gatctcctga taattcagaa
    30361 gcgaatggtt cgcctgcaaa taaaacagtg atgatatata gcgggaccat cacgaccatg
    30421 acagacaaat aagtatcttg cagaaattcg aaatgtgtcg gcaacttgat atcttcgatc
    30481 gactttttat tttcgccttt ctcaccaaaa agcttagcaa caccggcttc aaataagtac
    30541 ccgatcgtac aaaaatgacc taaagcaata tcatttgatc cagtgatttt acgaatgatc
    30601 ggttgcgcta ctgccggcat agcaatggcg aaacaagctc cgataaaacc accgaccaag
    30661 atcaatacga tccctcgcag accggcaaaa tagccaaaga ctgtcgtcat cgttgccatc
    30721 cataaaattg cttgaccagt taaaaaaatg tacttccatt ttgtgaaacg agccaaaata
    30781 atattgaata caaaaatggc taagaacgtc agtgcaatat ctctacctaa acccaaatca
    30841 ttcattgctt gaccattgat agattcaata gaagggatga tcccttgcat atgaaatcct
    30901 tcagtaaaga ttttcccgaa ataagttaaa cttccaacga tgatgctcga accggcactc
    30961 aacacttgaa atcctaggag tgtctttaac gtgccagaga tgacttgtcc tgctgatttc
    31021 ttctgcagta gtagacccaa catagcaatc aatgcaatgg tgatcgatgc ctgagtcaaa
    31081 atattttcga tgataaaatt gataacgccc atgtttttac cctcttttct ttttgtattt
    31141 agataactcc cttatcttgc agaactgggg tgattttttc ttcaatttca ttttttgaaa
    31201 caatattttt caaatagatc atcgccgttt taggatcaac tgcgaacttt tcgaactgcg
    31261 aacgaaaatt ttctgctgtg atgatcacat ctggtttcat ggaaactgcg gaagaaatat
    31321 cacaatggtc cagtttggct tctactccta atttgttcaa aacatcctca gcagacatct
    31381 gtgctgcaaa actacttcct aaacctgcac cgcatacgaa taaaatgttc aatttgttca
    31441 tctttgttct cccccgtatc atttattatt tctcaactac tgaaaaaagc agttgtttga
    31501 aatcttcgat atttgtgcat tcaatcaatc gatccaactt ctcttcgttg ttgatcaatt
    31561 caattagttc tttcatgatg ttcagatgcg agtaggagtc tactgctgcc aaacaaaaaa
    31621 taatctttac tggatccatt tcaggattac cgaaatcgat tggttgctca atcgtagcta
    31681 cgctgactcc taatttattg accccatctt ctggtcgggc atgggctagt gccatgtgtt
    31741 tgccaatcac gatatacggt ccgtaatcat tcactgcacg aatcatcgct tcgacatagc
    31801 gctcttcgac aatgttttct ttaaccaatg gtctagcagc tagttcaatc gattcctccc
    31861 aagtagtggc tttttctttg atcatgatat tgctgtccgt caaaatatct tttaacatag
    31921 gctggatctc ccttttgttg atttctaaat tgtttgtttc gaataatttt tctaacttgc
    31981 catagatgat tcgatctact cggccaccac tttctttcat gacatctatt atttgagtga
    32041 ataactccgt cgattcatga ctgttaggct ctaaccgctg tagttcttca tttcttttca
    32101 aaaagtcttc tataaccaag atattcgtct cagttaaaat cggatcgaca actaaaagcg
    32161 gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag attgacatcc aatttatcta
    32221 ctaattcgat ttcttttgaa cttaaaactg cagtaatctc gatctgagga aacttttctt
    32281 tcagattctc tgctaacaga tttccagtag ccatcccatg gttacagaca acgactgatt
    32341 tataaacata attcaagtct cgcttgatta cactggcgat cgtcgaaaag tggatgacaa
    32401 gaaaagctat ctcgtcctcc agtatcttgc caccaattac ttcttcgatc gtaggaacaa
    32461 aatgagtgac tgcgcggtag attgggaaat aatttgttcg gatgttttct ttcaatggat
    32521 tcatgacttg gatatgattc ttgattctta caatcagtgc agccatgtgc ttatagagat
    32581 tttcgaataa agcttcttcc tttagatgga aaggtacgtg tgtctcgttc tctacaaatt
    32641 ggatcaattg gatcgttagt aattgagctt gtacccactc gattgaattg gagatatctc
    32701 tagcattgaa cgtctcaata gtgaaacgaa gataatttct ttctacttgc gggacattgt
    32761 ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga aaagctactg tcttttattt
    32821 ctgtattttt ccaattaatg gcagagataa attcatgttt cctgattcgc tggatccaga
    32881 tcaatgtaaa aagcaggatt tgttttttat aaatatcatc ttcttgtttc aacaacttat
    32941 tggaatagat ctcctgtatt tttttgattt ccaccaaagg aaaataatgg agaaagattt
    33001 tttgagccgg tgttaattgt gcatcttgat tcataaagaa gtcggtcatt cccagattct
    33061 gattgataaa atcatagatc atcgtacgga tagatcgttc tgaaccttta taaaccagtc
    33121 cttgctttcc ataactgacg atctctatcc cataactgac cagtgttgct cgtaaccgtc
    33181 tcatatcttc atctaatgta cttttagaga tttgaaattc ttcttctttt ttgtttagat
    33241 ggatcgggat tcggctgtaa gcaacggcta aaactagatc aaaaatacgt tcgcgccgat
    33301 tcaaaaagcc gtctttcaca tcttttattt cagtttctaa tcgagcttgc ttttcctttt
    33361 ccagcttcaa caaaaaaact ttgttgcgaa ttgtacggat ctccggaagc tttttactgc
    33421 tcaaccagat attcgcttca agaacttcct gacggatcgt ccgctgactg acattgaact
    33481 cacttgctag atgttttgct tttactggat attgggctgt catgattctg tgaagtattt
    33541 caattgttct tgttttcata gaaatgatct ccttctgatt tcaagtatat ggttccgctt
    33601 acataataaa aagtagatat ttctgccact tgaagtggaa agaaagtaag caaagcaaac
    33661 aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa agacatacgc ctattattac
    33721 aaaaattttg cccatctata caaaaaagag gttgtgaaaa agctgtcctg catcaagtaa
    33781 taagaacaac taaactaaaa tagtgcttcc tatttttcgt ttggttgttc ttattatcgc
    33841 agatgcaagc ttttaaacac catttagtgg agaggttgtg acagagatgt tcagccttga
    33901 gcgataagaa gcgattttga aaaatcgctt cccatatttt acaaaatcgt gccttattgc
    33961 cgataggcta tctctggcac actgtttatt cagaaaaagg agctgcgaca aggcttttgt
    34021 cac
//
LOCUS       DACECJ010000022        33840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.022, whole genome shotgun sequence.
ACCESSION   DACECJ010000022 DACECJ010000000
VERSION     DACECJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.022"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            324..1985
                     /locus_tag="IWO09_001108"
     CDS             324..1985
                     /locus_tag="IWO09_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0263716.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2055..2402)
                     /locus_tag="IWO09_001109"
     CDS             complement(2055..2402)
                     /locus_tag="IWO09_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0263717.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2486..3427
                     /locus_tag="IWO09_001110"
     CDS             2486..3427
                     /locus_tag="IWO09_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263718.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3428..4471
                     /locus_tag="IWO09_001111"
     CDS             3428..4471
                     /locus_tag="IWO09_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263719.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4477..5547
                     /locus_tag="IWO09_001112"
     CDS             4477..5547
                     /locus_tag="IWO09_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263720.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5537..6490
                     /locus_tag="IWO09_001113"
     CDS             5537..6490
                     /locus_tag="IWO09_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0263721.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6502..7512
                     /locus_tag="IWO09_001114"
     CDS             6502..7512
                     /locus_tag="IWO09_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0263722.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7797..8618)
                     /locus_tag="IWO09_001115"
     CDS             complement(7797..8618)
                     /locus_tag="IWO09_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0263723.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8715..8993)
                     /locus_tag="IWO09_001116"
     CDS             complement(8715..8993)
                     /locus_tag="IWO09_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263724.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9261..11270
                     /gene="metG"
                     /locus_tag="IWO09_001117"
     CDS             9261..11270
                     /gene="metG"
                     /locus_tag="IWO09_001117"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0263725.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11749..12336)
                     /locus_tag="IWO09_001118"
     CDS             complement(11749..12336)
                     /locus_tag="IWO09_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263726.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12511..13833
                     /locus_tag="IWO09_001119"
     CDS             12511..13833
                     /locus_tag="IWO09_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0263727.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13988..14764
                     /locus_tag="IWO09_001120"
     CDS             13988..14764
                     /locus_tag="IWO09_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0263728.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14757..15326
                     /gene="rnmV"
                     /locus_tag="IWO09_001121"
     CDS             14757..15326
                     /gene="rnmV"
                     /locus_tag="IWO09_001121"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0263729.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15319..16203
                     /gene="rsmA"
                     /locus_tag="IWO09_001122"
     CDS             15319..16203
                     /gene="rsmA"
                     /locus_tag="IWO09_001122"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0263730.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16654..17283
                     /locus_tag="IWO09_001123"
     CDS             16654..17283
                     /locus_tag="IWO09_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263731.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17407..18516)
                     /gene="ugpC"
                     /locus_tag="IWO09_001124"
     CDS             complement(17407..18516)
                     /gene="ugpC"
                     /locus_tag="IWO09_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0263732.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18725..19147
                     /gene="mgsA"
                     /locus_tag="IWO09_001125"
     CDS             18725..19147
                     /gene="mgsA"
                     /locus_tag="IWO09_001125"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0263733.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19470..20006
                     /locus_tag="IWO09_001126"
     CDS             19470..20006
                     /locus_tag="IWO09_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0263734.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20046..21809)
                     /locus_tag="IWO09_001127"
     CDS             complement(20046..21809)
                     /locus_tag="IWO09_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263735.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21812..23527)
                     /locus_tag="IWO09_001128"
     CDS             complement(21812..23527)
                     /locus_tag="IWO09_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263736.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24306..24530)
                     /locus_tag="IWO09_001129"
     CDS             complement(24306..24530)
                     /locus_tag="IWO09_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263737.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24730..27498
                     /locus_tag="IWO09_001130"
     CDS             24730..27498
                     /locus_tag="IWO09_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0263738.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27579..27998
                     /locus_tag="IWO09_001131"
     CDS             27579..27998
                     /locus_tag="IWO09_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0263739.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            28010..28480
                     /locus_tag="IWO09_001132"
     CDS             28010..28480
                     /locus_tag="IWO09_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0263740.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28497..29276
                     /locus_tag="IWO09_001133"
     CDS             28497..29276
                     /locus_tag="IWO09_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0263741.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29263..30084
                     /locus_tag="IWO09_001134"
     CDS             29263..30084
                     /locus_tag="IWO09_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0263742.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30101..31150
                     /locus_tag="IWO09_001135"
     CDS             30101..31150
                     /locus_tag="IWO09_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0263743.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31163..32167
                     /locus_tag="IWO09_001136"
     CDS             31163..32167
                     /locus_tag="IWO09_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0263744.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32800..33819
                     /locus_tag="IWO09_001137"
     CDS             32800..33819
                     /locus_tag="IWO09_001137"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0263745.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11048 a         5996 c         6912 g         9884 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag
       61 tttctttttg tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat
      121 tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa
      181 ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat
      241 tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa
      301 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
      361 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
      421 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
      481 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
      541 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
      601 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
      661 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
      721 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
      781 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
      841 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
      901 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
      961 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
     1021 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
     1081 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
     1141 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
     1201 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
     1261 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
     1321 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
     1381 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
     1441 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
     1501 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
     1561 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
     1621 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
     1681 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
     1741 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
     1801 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
     1861 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
     1921 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
     1981 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
     2041 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
     2101 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
     2161 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
     2221 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
     2281 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
     2341 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
     2401 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
     2461 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
     2521 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
     2581 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
     2641 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
     2701 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
     2761 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
     2821 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
     2881 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
     2941 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
     3001 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
     3061 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
     3121 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
     3181 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
     3241 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
     3301 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
     3361 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
     3421 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
     3481 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
     3541 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
     3601 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
     3661 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
     3721 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
     3781 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
     3841 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
     3901 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
     3961 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
     4021 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
     4081 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
     4141 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
     4201 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
     4261 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
     4321 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
     4381 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
     4441 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
     4501 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
     4561 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
     4621 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
     4681 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
     4741 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
     4801 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
     4861 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
     4921 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
     4981 ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact
     5041 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
     5101 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
     5161 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
     5221 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
     5281 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
     5341 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
     5401 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
     5461 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
     5521 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
     5581 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
     5641 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
     5701 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
     5761 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
     5821 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
     5881 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
     5941 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
     6001 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
     6061 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
     6121 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
     6181 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
     6241 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
     6301 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
     6361 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
     6421 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
     6481 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
     6541 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
     6601 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
     6661 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
     6721 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
     6781 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
     6841 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
     6901 cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa
     6961 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
     7021 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
     7081 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
     7141 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
     7201 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
     7261 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
     7321 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
     7381 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
     7441 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
     7501 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
     7561 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
     7621 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
     7681 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
     7741 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
     7801 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
     7861 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
     7921 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
     7981 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
     8041 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
     8101 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
     8161 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
     8221 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
     8281 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
     8341 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
     8401 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
     8461 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
     8521 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
     8581 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
     8641 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
     8701 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
     8761 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
     8821 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
     8881 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
     8941 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
     9001 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
     9061 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
     9121 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
     9181 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
     9241 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
     9301 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
     9361 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
     9421 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
     9481 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
     9541 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
     9601 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
     9661 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
     9721 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
     9781 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
     9841 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
     9901 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
     9961 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
    10021 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
    10081 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
    10141 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
    10201 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
    10261 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    10321 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    10381 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    10441 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    10501 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    10561 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    10621 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    10681 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    10741 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    10801 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    10861 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    10921 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    10981 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    11041 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    11101 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    11161 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    11221 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    11281 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    11341 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    11401 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    11461 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    11521 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    11581 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    11641 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    11701 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    11761 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    11821 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    11881 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    11941 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    12001 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    12061 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    12121 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    12181 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    12241 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    12301 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    12361 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    12421 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    12481 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    12541 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    12601 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    12661 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    12721 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    12781 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    12841 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    12901 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    12961 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    13021 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    13081 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    13141 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    13201 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    13261 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    13321 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    13381 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    13441 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    13501 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    13561 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    13621 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    13681 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    13741 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    13801 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    13861 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    13921 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    13981 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    14041 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    14101 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    14161 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    14221 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    14281 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    14341 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    14401 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    14461 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    14521 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    14581 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    14641 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    14701 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    14761 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    14821 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    14881 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    14941 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    15001 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    15061 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    15121 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    15181 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    15241 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    15301 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    15361 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    15421 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    15481 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    15541 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    15601 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    15661 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    15721 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    15781 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    15841 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    15901 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    15961 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    16021 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    16081 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    16141 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    16201 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    16261 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttgttac cgcagatgca
    16321 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    16381 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    16441 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    16501 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    16561 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    16621 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    16681 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    16741 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    16801 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    16861 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
    16921 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    16981 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    17041 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    17101 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    17161 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    17221 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    17281 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
    17341 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
    17401 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
    17461 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
    17521 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
    17581 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
    17641 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
    17701 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
    17761 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
    17821 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
    17881 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
    17941 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
    18001 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
    18061 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
    18121 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
    18181 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
    18241 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
    18301 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
    18361 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
    18421 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
    18481 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
    18541 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
    18601 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
    18661 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
    18721 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    18781 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    18841 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    18901 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    18961 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    19021 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    19081 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    19141 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    19201 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    19261 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    19321 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    19381 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    19441 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    19501 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    19561 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    19621 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    19681 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    19741 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    19801 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    19861 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    19921 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    19981 gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct
    20041 tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga
    20101 agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg
    20161 tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc
    20221 attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct
    20281 tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg
    20341 cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc
    20401 aaacgttcta caaattcata catataagag gcttttaggg catcattgat ttctgcgtca
    20461 cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta
    20521 tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca
    20581 tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca
    20641 agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca
    20701 gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca
    20761 aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc
    20821 ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc
    20881 agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc
    20941 gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg
    21001 ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc
    21061 aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca
    21121 aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt
    21181 cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt
    21241 tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg
    21301 accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc
    21361 actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca
    21421 tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat
    21481 cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga
    21541 ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat
    21601 agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg
    21661 atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt
    21721 atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc
    21781 gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt
    21841 cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac
    21901 cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa
    21961 taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat
    22021 aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc
    22081 ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt
    22141 ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag
    22201 cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag
    22261 tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca
    22321 gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg acatcataga
    22381 aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa
    22441 ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag
    22501 tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag
    22561 tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc
    22621 ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa
    22681 gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat
    22741 ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg
    22801 tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat
    22861 ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac
    22921 gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt
    22981 tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca
    23041 ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc
    23101 cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa
    23161 gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact
    23221 cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc
    23281 cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag
    23341 acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc
    23401 cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa
    23461 atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact
    23521 gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac
    23581 attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt
    23641 attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa
    23701 aacatgccag tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa
    23761 ttctgcataa aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt
    23821 taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa
    23881 atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt
    23941 gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta
    24001 tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt
    24061 gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt
    24121 aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag
    24181 tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt
    24241 tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt
    24301 gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa
    24361 aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata
    24421 gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga
    24481 aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca
    24541 tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg
    24601 ttttttagaa taaaaattat aagggataag taggctgctg tctataataa gatatatttt
    24661 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
    24721 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
    24781 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
    24841 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
    24901 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
    24961 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
    25021 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
    25081 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
    25141 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
    25201 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
    25261 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
    25321 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
    25381 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
    25441 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
    25501 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
    25561 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
    25621 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
    25681 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
    25741 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
    25801 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
    25861 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
    25921 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
    25981 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
    26041 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
    26101 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
    26161 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
    26221 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
    26281 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
    26341 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
    26401 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
    26461 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
    26521 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
    26581 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
    26641 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
    26701 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
    26761 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
    26821 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
    26881 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
    26941 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
    27001 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
    27061 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
    27121 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
    27181 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
    27241 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
    27301 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
    27361 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
    27421 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
    27481 atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc
    27541 ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag
    27601 cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa
    27661 atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag
    27721 taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg
    27781 gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg
    27841 ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga
    27901 caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg
    27961 agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt
    28021 gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat
    28081 aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac
    28141 aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc
    28201 gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc
    28261 gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg
    28321 agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga
    28381 agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt
    28441 accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga
    28501 ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag
    28561 ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg
    28621 atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct
    28681 cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg
    28741 ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt
    28801 tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag
    28861 cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg
    28921 gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca
    28981 atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag
    29041 ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac
    29101 aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga
    29161 ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc
    29221 gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc
    29281 aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg
    29341 gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg
    29401 attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta
    29461 gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg
    29521 gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca
    29581 tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg
    29641 attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg
    29701 ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga
    29761 tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg
    29821 acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc
    29881 accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt
    29941 tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta
    30001 ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt
    30061 ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta
    30121 tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag
    30181 agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc
    30241 ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga
    30301 acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt
    30361 gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg
    30421 gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca
    30481 aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc
    30541 gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag
    30601 caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc
    30661 gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca
    30721 gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa
    30781 attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg
    30841 cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa
    30901 taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac
    30961 cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga
    31021 atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt
    31081 agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag
    31141 taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat
    31201 aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga
    31261 aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc
    31321 tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata
    31381 ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac
    31441 cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact
    31501 gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac
    31561 ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta
    31621 tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc
    31681 aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa
    31741 acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta
    31801 ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc
    31861 tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt
    31921 tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat
    31981 ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa
    32041 atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc
    32101 tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga
    32161 ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc
    32221 cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc
    32281 tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg
    32341 caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc
    32401 gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa
    32461 ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat gttcaatctg
    32521 tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa
    32581 tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg
    32641 taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa
    32701 agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact
    32761 ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt
    32821 cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat
    32881 gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat
    32941 cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca
    33001 gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca
    33061 gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa
    33121 ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa
    33181 gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta
    33241 catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa
    33301 aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa
    33361 actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga
    33421 ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt
    33481 ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg
    33541 atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag
    33601 ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg
    33661 acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca
    33721 atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta
    33781 cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc
//
LOCUS       DACECJ010000023        33737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.023, whole genome shotgun sequence.
ACCESSION   DACECJ010000023 DACECJ010000000
VERSION     DACECJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.023"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IWO09_001138"
     CDS             214..498
                     /locus_tag="IWO09_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0263746.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IWO09_001139"
     CDS             complement(779..1198)
                     /locus_tag="IWO09_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0263747.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1200..1784)
                     /locus_tag="IWO09_001140"
     CDS             complement(1200..1784)
                     /locus_tag="IWO09_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0263748.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IWO09_001141"
     CDS             complement(1831..3306)
                     /locus_tag="IWO09_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0263749.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IWO09_001142"
     CDS             3487..3798
                     /locus_tag="IWO09_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263750.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IWO09_001143"
     CDS             complement(3913..6249)
                     /locus_tag="IWO09_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0263751.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IWO09_001144"
     CDS             6391..7059
                     /locus_tag="IWO09_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0263752.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IWO09_001145"
     CDS             7062..8105
                     /locus_tag="IWO09_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0263753.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IWO09_001146"
     CDS             complement(8342..9223)
                     /locus_tag="IWO09_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0263754.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IWO09_001147"
     CDS             9382..10110
                     /locus_tag="IWO09_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0263755.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IWO09_001148"
     CDS             10266..10583
                     /locus_tag="IWO09_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0263756.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IWO09_001149"
     CDS             10576..11184
                     /locus_tag="IWO09_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0263757.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IWO09_001150"
     CDS             11200..12030
                     /locus_tag="IWO09_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0263758.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12680
                     /locus_tag="IWO09_001151"
     CDS             12168..12680
                     /locus_tag="IWO09_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0263759.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="IWO09_001152"
     CDS             12706..13578
                     /locus_tag="IWO09_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0263760.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="IWO09_001153"
     CDS             13754..14116
                     /locus_tag="IWO09_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0263761.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="IWO09_001154"
     CDS             14125..14877
                     /locus_tag="IWO09_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263762.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="IWO09_001155"
     CDS             14891..16693
                     /locus_tag="IWO09_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0263763.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="IWO09_001156"
     CDS             16970..18010
                     /locus_tag="IWO09_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0263764.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18062..20206)
                     /locus_tag="IWO09_001157"
     CDS             complement(18062..20206)
                     /locus_tag="IWO09_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0263765.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20563..21936)
                     /locus_tag="IWO09_001158"
     CDS             complement(20563..21936)
                     /locus_tag="IWO09_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0263766.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="IWO09_001159"
     CDS             complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="IWO09_001159"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0263767.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="IWO09_001160"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="IWO09_001160"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0263768.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="IWO09_001161"
     CDS             complement(23384..24397)
                     /locus_tag="IWO09_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0263769.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="IWO09_001162"
     CDS             complement(24478..25077)
                     /locus_tag="IWO09_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0263770.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="IWO09_001163"
     CDS             25263..25964
                     /locus_tag="IWO09_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263771.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="IWO09_001164"
     CDS             25974..28685
                     /locus_tag="IWO09_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0263772.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29082..30710
                     /gene="treC"
                     /locus_tag="IWO09_001165"
     CDS             29082..30710
                     /gene="treC"
                     /locus_tag="IWO09_001165"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0263773.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30770..31951)
                     /locus_tag="IWO09_001166"
     CDS             complement(30770..31951)
                     /locus_tag="IWO09_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0263774.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32203..33726
                     /locus_tag="IWO09_001167"
     CDS             32203..33726
                     /locus_tag="IWO09_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0263775.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        10691 a         6051 c         6863 g        10132 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cgacaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tatgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatgtttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattc caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcactcatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgaa ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg atccgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atctgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattggac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aaggggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcat
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat gggaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    18061 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    18121 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    18181 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    18241 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    18301 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    18361 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    18421 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    18481 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    18541 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    18601 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    18661 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    18721 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    18781 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    18841 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    18901 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    18961 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    19021 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    19081 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    19141 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    19201 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    19261 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    19321 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    19381 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    19441 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    19501 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    19561 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    19621 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    19681 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    19741 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    19801 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    19861 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    19921 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    19981 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    20041 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    20101 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    20161 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    20221 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    20281 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    20341 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    20401 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    20461 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    20521 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    20581 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    20641 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    20701 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    20761 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    20821 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    20881 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    20941 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    21001 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    21061 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    21121 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    21181 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    21241 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    21301 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    21361 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    21421 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    21481 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    21541 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    21601 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    21661 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    21721 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    21781 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    21841 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    21901 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    21961 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    22021 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    22081 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    22141 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    22201 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    22261 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttttg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    28741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    28801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    28861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    28921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    28981 taaattttac aaacgctttc tgttcagtta taataataaa tgataacttg tatatataag
    29041 ctgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    29101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    29161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    29221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    29281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    29341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    29401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    29461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    29521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    29581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    29641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    29701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    29761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    29821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    29881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    29941 attttgcaga attaaagcga ctatttcata catggggaga aaatatgagt gagggtaacg
    30001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    30061 ttatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    30121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    30181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    30241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    30301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    30361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    30421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    30481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    30541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    30601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    30661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    30721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    30781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    30841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    30901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    30961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    31021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    31081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    31141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    31201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    31261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    31321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    31381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    31441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    31501 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    31561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    31621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    31681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    31741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    31801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    31861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    31921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    31981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    32041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    32101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    32161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    32221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    32281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    32341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    32401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    32461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    32521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    32581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    32641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    32701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    32761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    32821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    32881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    32941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    33001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    33061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    33121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    33181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    33241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    33301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    33361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    33421 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    33481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    33541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    33601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    33661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    33721 cactgatagg caaaaaa
//
LOCUS       DACECJ010000024        32406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.024, whole genome shotgun sequence.
ACCESSION   DACECJ010000024 DACECJ010000000
VERSION     DACECJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.024"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            157..2055
                     /locus_tag="IWO09_001168"
     CDS             157..2055
                     /locus_tag="IWO09_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0263776.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2354..3073)
                     /locus_tag="IWO09_001169"
     CDS             complement(2354..3073)
                     /locus_tag="IWO09_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0263777.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3410..3742
                     /locus_tag="IWO09_001170"
     CDS             3410..3742
                     /locus_tag="IWO09_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263778.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3729..5732
                     /locus_tag="IWO09_001171"
     CDS             3729..5732
                     /locus_tag="IWO09_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0263779.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5751..6221
                     /locus_tag="IWO09_001172"
     CDS             5751..6221
                     /locus_tag="IWO09_001172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0263780.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6242..6826
                     /locus_tag="IWO09_001173"
     CDS             6242..6826
                     /locus_tag="IWO09_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0263781.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6841..7827
                     /locus_tag="IWO09_001174"
     CDS             6841..7827
                     /locus_tag="IWO09_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0263782.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7817..8128
                     /locus_tag="IWO09_001175"
     CDS             7817..8128
                     /locus_tag="IWO09_001175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0263783.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8150..9931
                     /locus_tag="IWO09_001176"
     CDS             8150..9931
                     /locus_tag="IWO09_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0263784.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9924..11300
                     /locus_tag="IWO09_001177"
     CDS             9924..11300
                     /locus_tag="IWO09_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0263785.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11307..11942
                     /locus_tag="IWO09_001178"
     CDS             11307..11942
                     /locus_tag="IWO09_001178"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0263786.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11972..13327
                     /locus_tag="IWO09_001179"
     CDS             11972..13327
                     /locus_tag="IWO09_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0263787.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13519..14913)
                     /locus_tag="IWO09_001180"
     CDS             complement(13519..14913)
                     /locus_tag="IWO09_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0263788.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            15005..15976
                     /locus_tag="IWO09_001181"
     CDS             15005..15976
                     /locus_tag="IWO09_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0263789.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15973..16755
                     /locus_tag="IWO09_001182"
     CDS             15973..16755
                     /locus_tag="IWO09_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0263790.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16924..17295)
                     /locus_tag="IWO09_001183"
     CDS             complement(16924..17295)
                     /locus_tag="IWO09_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0263791.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17429..17872
                     /locus_tag="IWO09_001184"
     CDS             17429..17872
                     /locus_tag="IWO09_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0263792.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17915..18724)
                     /gene="yidA"
                     /locus_tag="IWO09_001185"
     CDS             complement(17915..18724)
                     /gene="yidA"
                     /locus_tag="IWO09_001185"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0263793.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18743..20110)
                     /locus_tag="IWO09_001186"
     CDS             complement(18743..20110)
                     /locus_tag="IWO09_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0263794.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20094..20924)
                     /locus_tag="IWO09_001187"
     CDS             complement(20094..20924)
                     /locus_tag="IWO09_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0263795.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21087..21524
                     /locus_tag="IWO09_001188"
     CDS             21087..21524
                     /locus_tag="IWO09_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0263796.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21600..22220
                     /locus_tag="IWO09_001189"
     CDS             21600..22220
                     /locus_tag="IWO09_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0263797.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22409..22915
                     /locus_tag="IWO09_001190"
     CDS             22409..22915
                     /locus_tag="IWO09_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0263798.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22932..23831
                     /locus_tag="IWO09_001191"
     CDS             22932..23831
                     /locus_tag="IWO09_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0263799.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23918..24802
                     /locus_tag="IWO09_001192"
     CDS             23918..24802
                     /locus_tag="IWO09_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0263800.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24994..25065)
                     /locus_tag="IWO09_001193"
     tRNA            complement(24994..25065)
                     /locus_tag="IWO09_001193"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25030..25032),aa:Arg,seq:ccg)
     gene            25125..26459
                     /gene="rpoN"
                     /locus_tag="IWO09_001194"
     CDS             25125..26459
                     /gene="rpoN"
                     /locus_tag="IWO09_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0263801.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26662..27699
                     /locus_tag="IWO09_001195"
     CDS             26662..27699
                     /locus_tag="IWO09_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0263802.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            27740..28741
                     /gene="gap"
                     /locus_tag="IWO09_001196"
     CDS             27740..28741
                     /gene="gap"
                     /locus_tag="IWO09_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0263803.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28851..30041
                     /locus_tag="IWO09_001197"
     CDS             28851..30041
                     /locus_tag="IWO09_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0263804.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30145..30900
                     /locus_tag="IWO09_001198"
     CDS             30145..30900
                     /locus_tag="IWO09_001198"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0263805.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            31016..32314
                     /gene="eno"
                     /locus_tag="IWO09_001199"
     CDS             31016..32314
                     /gene="eno"
                     /locus_tag="IWO09_001199"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0263806.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        10689 a         5733 c         6895 g         9089 t
ORIGIN      
        1 gaaaaatata gaaggaagct ggacatttat tgttcccagc ttcttttttg tatagaccta
       61 tttctatttt tttattatat tggatatata tattcattga aaatagagca taacaacttt
      121 ataatgttga gtataccaat ttttggaggc gttttaatgg accgctattt agatttactt
      181 tcagagaagt tccctacaac ggaagatgtc atcacggaaa tcatcaatct cgaagcaatt
      241 ttgcagcttc caaaaggaac agagttattt ttgagcgata tacatggcga atttcctgct
      301 tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttc
      361 tctgaacaaa tgacggaaac ggagatcagc caattcacta tttttacggc ttatccagaa
      421 tatgtgttga cgactgattg gtacaaaaaa caagataaat cccagctgat ccatcgtttg
      481 atcgatcttt tacggtttac gacagtcaaa tacactcgat caaaagttcg aaaaggattg
      541 ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgatcg tatcaacggg
      601 aaaaaagact atgtaaaaaa aatcattgaa caacttgtct tgatgaagga agaagaaaaa
      661 tttttaacaa aacttgcgca gacgatccaa aaatctgtca tcgatcattt gcatatcgtg
      721 ggtgatattt ttgatcgagg aactcaagca gccaaagtaa tggatcgaat catggatttt
      781 tcctctgtag atatccagtg ggggaatcat gatattttgt ggattggtgc gtattgcggc
      841 tctgaggcat gtttattgac gcttttacgg attgccgcac gctacaacta tttatttgaa
      901 ttagagaaag aatatggatt aaatttacgt ccattatttt cttttgccca tcaaacctat
      961 cagaagaatc cagtatttac ccctaaaaac aggaaagatc tatctgaaag agagcaagaa
     1021 gaattagaaa agatccatca agcactttct atcctccaat tcaaatcaga acatcagttg
     1081 ttgaatcgac gacctgaatt taaaatggac gatcgtaaat tgttggaaaa aatcaactat
     1141 gaggaatcaa cgatcgtttt agaaggtcag ctctatgagt tgaaagggac ttgttttcaa
     1201 acgatccagc cagatgatcc taaaaaattc ttggaagagg aacaaaaagt gatcgatagt
     1261 ttgatgtatt cattccagca ctcgttaaga atgcaaaaac acatgacgtt tttgatcgaa
     1321 aaagggggaa tgtatctcac gaacaatcag catgtactgt tccatggctg tatacctgta
     1381 gatgaaaagg ggcagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg
     1441 ctgggatttt ttgaaaaaca gataactgaa agcgcaaaag atttgactgc aaatgaagat
     1501 ttggcgaccg atttgatttg gtatgcgtgg agcgggccat tttcgccttt attcggcaag
     1561 agtaaaatgg caacttttga gcgctatttt ctcacagagc ctcatacgca cgtcgaaaaa
     1621 agcaatccct attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttggcagaa
     1681 ttcgatgcaa aagaagaagg ttcagcaatc atcaatggac acacgccagt aaaagtaaaa
     1741 aaaggagagt caccgatcaa agcaaacgga aaagcatttg ttattgacgg aggtctatcc
     1801 aaagcttatc aaaaaacaac cgggattgct ggctactcat tactatgcaa ttcttacggt
     1861 ttccaaatcg tgacacatta tccgttcttg cctattgaac aacaatttgc aaaaaaatct
     1921 gatcaaacga atgtaaaaaa agtgatcgag cagcagctcc cacgcaaact taatcggaat
     1981 acgacaaaag gaatggaatt acagcaacag attactcaac tccaaaggct gttggattat
     2041 cgaaaggacg attgagaaaa atgctagcca acattgctca atagactaaa aagcgactgc
     2101 aacaaaaggc gtgtcgcagt ctccttcgga attagctcaa aaaacggtaa aatattagaa
     2161 gctatttttc tgtttatttc tctaatactc aggtcagggc gctgatttca cgacctcttc
     2221 cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca acaaacatga
     2281 gaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgaccta
     2341 atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa taaacgctgt tcagatgaat
     2401 ctacacgatg agaaagttcc ggatgccatt ggacaccaag gattttgtgc atattatctt
     2461 tactttggac tgcttcaatg agtccatcgt tggaaaaggc gatggctttt aacaaaggag
     2521 aaagatcttt gactgcctga tgatgatagg aattcacaaa gtatgaatca gggagaaggt
     2581 cacttaataa actgtccgaa acgattttga cttcgtgagt tgcaaactgc ggggcagtgg
     2641 gctgttgttc gtgttttacg gaccattttg gatataatga aagatcttga taaagtgttc
     2701 cctccaacac gacattcaat agttgaagtc ctcggcagac tgcaaaaatc ggttttcttt
     2761 gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc tcgttgaata tttgtttccg
     2821 tcagttttgg atgagggtct tccatgtaaa gttgcggact aacatcctga ccgcctgcaa
     2881 gaatgagttt gtcgattttc tcaattagtt gaggcgctga atctggagac atgactggca
     2941 gaataagagg taatccttga gcatctaaaa ctgcatccac aaatccctgc ggtgtatagc
     3001 ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag ttgattcccg gcaatgccga
     3061 tgattggttt catttttatt ccccctaaag tagactaatt gtaacaagga tttaaaaaaa
     3121 tttaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta ctatctgtaa
     3181 agagaatttt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat ttgttataat
     3241 aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt tcttcaaggg
     3301 gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta gaggtacata
     3361 ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag tgaagcaagt
     3421 gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgagaagc gtgctcgaac
     3481 cctacggcag cagttgaaga cctatgcaga agaaaaagag caagaattgg agataatcca
     3541 aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa aaagaacaat tcgaaaagca
     3601 agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc aagcaacttt
     3661 gaaagaccaa tatcaaaagc atcagagtca agcaatcggt gcaatcattg aaagggtgaa
     3721 agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat aaaagaaatc
     3781 aggagaggat tttacaagca gtacaaggaa tccaaaatgt tgaaatccgg gatctgtatc
     3841 aaaatagaac aaacaaccag tgggtagaaa aatattttcc agataccacg attattgata
     3901 aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga catccaagaa gctatccaat
     3961 tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacgt caagaacttt
     4021 ccttacaaga cttggaagca aattattccg aagaaaattt catgaataaa ttagaagaag
     4081 tccttgattt aaaagaaaag tgggaacagc taactgaaaa acgtgatcgt ttagaagaag
     4141 aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg tatgcaagta
     4201 agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag
     4261 aactaagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca aaacaagttt
     4321 atctttcgta tgttgcttta aaaaaatcag ttgctgctat agaagaaatt gctgggaaat
     4381 acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa ttgcaagaaa
     4441 ccaaacagca attatccatt gttcacgagc agcaaaaaaa gataacgaca gcgttaggtc
     4501 agtgcagcgg ctatatccaa gaatttgaat gggtagaaga agtcacactt gccgttgcag
     4561 aacgggaaaa agtaaaagaa cggtttatcc atgccactta tctgatcgtt ttgcaaggtt
     4621 gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt aaacgaaact gtatcagaag
     4681 aagatatata cgttagtttc gaaacaccaa actcagaaga gattcagcag gaagtcccta
     4741 ctaaattgaa aaaccatccg cttatcgaac cttttgaatt gctgactgaa atgtacagtc
     4801 tgccaaaata tgaagaagta gatccaacgc cttggatgac accattttat cttgtcttct
     4861 tcgggatgat ggtcgcagat gttggttacg gattgctgat gctcattggg acaatactag
     4921 ctcaaaaact atttgtttta ccccgcggga tgaaacgttt cgtaaaattc tttgagattc
     4981 tctcggtacc atcgatcatc tggggattga tctacagttc atttttcgga caggcattac
     5041 caaaagaact atttggtatc cgcttgcctt tcccaatcct atctacgacg gatgacgtca
     5101 atacgatttt gattttatcc gtgattttcg gtctgataca gatcttggtt ggattattcg
     5161 tggcagcaaa ggaaaatatc aggaggaaag attacttgag tgctgtcagt gatggttttg
     5221 cctggcaagg gatcctgatt gggatcgtta ttgcattgat tggtgtcatg ttgataaaaa
     5281 atacagcatt tgtttatctt ggcgcggggc tatcgatcat tgctgcttta tgtatcttaa
     5341 tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc tgccaaagga gcgtataatc
     5401 tttacggatt gactggttat atcggtgatt tagtcagtta tacacgtttg atggcactag
     5461 gtatttcagg cggaagtatt ggcgcggcat tcaatatgct agttgctttc atgccgccag
     5521 ccgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg aatatgtttt
     5581 tgacactgtt aagtgcctat gtccatggtg cacgattgca gtatgtcgaa ttttttggaa
     5641 aattttatac aggaggcgga cgtgcctttg acccgctgaa aacggcggaa aaatacgtca
     5701 acatcaatca taagaaaaaa aacaataaat aattggagga tttttataca atgatggatt
     5761 acttaattag tcaaaatggt ggaatggtat ttgcagtatt agcgatggca acagcgacga
     5821 ttttttcagg gattggttca gctaaaggtg ttggaatgac aggtgaggca gcagcagcgt
     5881 taacaacaag tcaacccgaa aaattcggac aggctttgat tctgcaattg cttccaggta
     5941 cgcaagggtt gtatggattc gttatcgcct ttttgatttt tatcaactta ggaaatgata
     6001 tgtcagttgt acaaggattg aactttctag gcgcttctct gccgattgct tttacaggtt
     6061 tgttttcagg tatcgcccaa gggaaagttg cagcagcagg tatccaaatt ttagcgaaaa
     6121 aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca tatgcgattt
     6181 taggtttcgt tatttccttc ctattagtat tgaatgccta agcataaagg aggaaaagca
     6241 gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa gcagcagagc aagaaagagc
     6301 ggcgttagag caagaagagc gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca
     6361 aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattacttg aaaaaaaata
     6421 ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa gaaaatttga atgcgaagca
     6481 ggaattttta cgacgattgt ttgcagatgc aatagctgaa atggagaatt gggatgaatc
     6541 tgaacaaatc cagtttatca aaaattcatt gtattcatta cctttgacag gcaaagtaac
     6601 attcattgct ggagaaaaat cagcagctta tctttctcag acattgctgg atgaatggaa
     6661 caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc aagcgggttt
     6721 tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg tacaagatat
     6781 ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag ggatcagtgt
     6841 atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact tttgtccaat
     6901 gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga aatattgaaa
     6961 tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga acagaatctt
     7021 ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc
     7081 gtttggatct atacgatgcg ctatactttc cataatctga aggttttgac aaaagccgag
     7141 atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc attagaaacc
     7201 ttgaaggatg ccattcatac gcaagtttca aatgaactcc ctgaaagcat tatggacagt
     7261 atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat tgacgtgatt
     7321 tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg atatcaagaa
     7381 ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat ggctcgagga
     7441 atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc gggaagtatc
     7501 gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac gaattttcta
     7561 ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa tcttgatctc
     7621 gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga agcacaaacc
     7681 caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt agaaagcaaa
     7741 aaccttcgtt tactggttat cggcaaaaga agccatttcg aaccagaaac aatcagaaag
     7801 cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag attctgtttt
     7861 gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca
     7921 ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag agcaatgtgc
     7981 agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg ctatcgtatt
     8041 gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa aaaacgtaga
     8101 aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt tgcaaattgg
     8161 aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg atgccagtat
     8221 ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg agatgcgtgg
     8281 cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag gcgaaccggt
     8341 tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt cacaaatgtt
     8401 tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta atttcttagg
     8461 gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg aaccaacagt
     8521 agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag aaacaaaagt
     8581 tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa
     8641 aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa
     8701 tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa
     8761 cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc cagtgacaaa
     8821 aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca
     8881 aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa
     8941 tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata caggagaatc
     9001 gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtag cagctcgaga
     9061 agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg gttatgacgt
     9121 tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga tgagcggacg
     9181 tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac ggcttgcgga
     9241 atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg aaggaagtat
     9301 aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag tcacacaaaa
     9361 tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc aaaaaagaca
     9421 ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag tagggaagta
     9481 tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca tgcgtatttt
     9541 gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt ccctttcaga
     9601 taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt tgcagcaaaa
     9661 tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa tgttgaagat
     9721 gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga
     9781 gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat atatcccgga
     9841 aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga tgcaaacgat
     9901 cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg
     9961 gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc
    10021 gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa gataaagcaa
    10081 tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct gtacgatttt
    10141 taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt
    10201 tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg gatattaacg
    10261 gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa acaggtatct
    10321 ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta ttttctggct
    10381 ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg gtactcgatt
    10441 catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa gaagctgaat
    10501 ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg tttatgaatt
    10561 tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg actgctgcag
    10621 aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac atgaccaact
    10681 atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga agaagaggat
    10741 atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga agaatccgcg
    10801 gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac gataaaactc
    10861 atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg actagagagc
    10921 tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg cggttaaaag
    10981 ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg aatcagcttt
    11041 ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta ggtgaatctg
    11101 ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa aaagaatatg
    11161 tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta gggtgggaac
    11221 tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg ctggacaaat
    11281 atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg acccgcatgg
    11341 aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa ttattaaaag
    11401 ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac aaccagctgc
    11461 gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg gcaaatgctt
    11521 cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac gttgatttgt
    11581 ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt gaatatgatg
    11641 aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc gaactggatc
    11701 ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca gaaatcgaaa
    11761 aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc aatgcattag
    11821 aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg aagctggaag
    11881 aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt accactgact
    11941 aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac aaataaagaa
    12001 acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt tagttggcgg
    12061 cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt ttatcgatgc
    12121 gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga atacagcaga
    12181 gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg gcggtcttgg
    12241 ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca gtctcagcac
    12301 acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga tgaagctcat
    12361 gctttacatc ctaaaattgg cctttgttat ccaattgatt ggtacggttg ctttatcttt
    12421 tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt tccatgcaat
    12481 ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca atcaccagca
    12541 aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt taggcttcat
    12601 cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc attcaaaaat
    12661 cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct ttttcacaga
    12721 gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa acaccttttt
    12781 tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc atatgagcca
    12841 tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg ggtcgacggc
    12901 cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa
    12961 aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag tgttgcgggc
    13021 acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt
    13081 gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg tcttctctgc
    13141 gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa tagggaaatt
    13201 gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg tcttctctct
    13261 gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat
    13321 tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa aataaaataa
    13381 tttttcttgc ttcttggata ctttaagtgc aaaatgttcg tttatttttt gaggcactac
    13441 ccgttgttga acctcgggta gtgcctcttt tttattgaaa ggaatcaata agatttgatt
    13501 cctttattta tcaataggtt ataattcata tttttttatt ttttctttta cgatacttag
    13561 tccagaagct aaaatttcaa ggttattaga ggatagggaa attcgaacga atgcatctgg
    13621 ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg ataccgttag caagaaagtc
    13681 attctcaatt atcttagggt caatctcagg atcaactgga agaattcgat agtaagggac
    13741 acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa agctgattgg ctctttttgt
    13801 aagacttaat ttagtttcta agatcctatt tgcttcattt gaatggagga tttgagtgat
    13861 gacttccaca tctaatccag aagttttcac atttatatta aaaattgctt gttctatctt
    13921 gctttggaca tgtactggaa ctacaagata agcaactcgc aatccagaac aaataaattt
    13981 tgtcattcca gctaagtaga ttgtttggtt aggtaataaa ttttggaaag gaggaatgat
    14041 cgtatgttga tagtaagttg ttaaaaatga atggatatca tcttcaataa cccatagacc
    14101 ttctttttca atcacttcgg ctaattgctc tcgtcttttg tgagagattt gaaaccctat
    14161 aggattatta caagaaggca taagaaagac accatgaatc ttcttttttc tacattcctg
    14221 aatcaatata tcaggtcgca ttccttgctc gtcaaaatca attggaacga tctctaaatg
    14281 atataactga gccaattcaa tgaaattagc gtatgtatat cggtctgttg ctattctatc
    14341 acctggagag aaaagtgcag aaagtgtgat agccaatcca ttttgaacac cggaaacaat
    14401 agatagagat tctaaggacg gaatgactcc ttgagttttt aaccaatttg ctgcagtagt
    14461 caattgaaaa tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa gatttgggtt
    14521 attactagtt gattgaataa acgggacaac gaatttatta cattcttcaa aagaactaac
    14581 cagaccaaaa tcaatgatat gttcggttac ttcattggta gagatggtga tggactcaaa
    14641 gacattggga gaaataaatg tgccacttcc aatattcgta tacagcaaac ctttctcaat
    14701 tccatattta taagccttgc caacggtagt aaaatttaaa tctaaataat cagctagctc
    14761 tctttgggag ggtaactgag tgtttttctg taatttattc tttaaaatat cttgttctaa
    14821 ttgttgaatc aacgatcgat aataaggacg cttcaatttt tgtttgtctg gtcgccaagt
    14881 taacgggtaa ttgtcataag aattaatagg cattttctcc actccttaaa attgtatacc
    14941 atacaatttt accattgatt gtattgattt tagaagttaa tcttttgtga ggagggaatt
    15001 gtctatgaaa attattaatt ttgaggaagt agtagcttct tttagttttg aagaagcgat
    15061 tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg
    15121 attggtggaa attttacctg acggaaaagg caaaaatatg tttgcgatga tgcctgctta
    15181 tttgggggag aacagatatt ttggcacaaa agtcattacc gcttttccgg ataatcatca
    15241 gtatcattta ccttctcatt taggagagat tttcttgttt gatgctcaaa acggactacc
    15301 caaagcattg ataaacgcaa atgcagtcac ctggattcga acagctgctg tatctgcgct
    15361 ggcaacagat tacttagcaa atccgcatgc agaaacactt gctttaattg gagctggtca
    15421 acaagcattg tcgcatttga ctgcgatttc taccattcgt cccatcacta cagtgtttgt
    15481 ttacgatatt ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa aacagtatcc
    15541 taaaataacg tttataaatg caatatcagt agaagatgca gtacgtccag cggaaatcat
    15601 ttgtacgttg acctctagta aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc
    15661 acatatcaat gctgtgggca catttactcc tgatacaaga gagataagta catcgttgat
    15721 ggaaacttct caaatctatg tagatgatta ctcagctgcc ttaacagaaa gcggggacct
    15781 gcttatagcc atggcagaag gagatttaga ggaatcttgt attgttggta cattaggcga
    15841 attagtttct gagaaaaaaa cattttataa aggagaatcg aggccgacga ttttcgacgc
    15901 agttggtcta gctattgaag atctttgttg tgcagaatat atttataaca aattagagaa
    15961 aggggcttta aaatgaactt agattattac gtagttgatg catttgcaga tgaggtgttc
    16021 aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa
    16081 aaaattgcca ttgaaaacaa tttatctgaa acagcattta cagtgaagaa aaaccaagaa
    16141 tttgagctta gatggtttac gccagatcga gaaatcgatc tttgtggtca cgcgacatta
    16201 gcaacagcat ttgttttatt taactattac aaaatacctg atgaaacgat taaattttct
    16261 actcaaagcg gtaatctatt tgtgacaaga attaaagatt attattacat ggattttcct
    16321 agtattatgc cgaaaaaagt gccgatattg tctgaatatg aagaagcgat tggggctaag
    16381 attaaagaag cctacttagc acgtgattta tttttcgtat tagaagatga aaaaacggtg
    16441 gctaaattgc agccagattt tacagctatc aaaaattttg aattaggaat aggagtcatc
    16501 gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc aaaattaaca
    16561 atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta ttgggcagaa
    16621 agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg ggatctagaa
    16681 tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt ttctaaaggt
    16741 acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag agtggcaacc
    16801 ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg
    16861 cgtctcattt taattattgg tgtttgtttt ttattcaatc aagagtaaaa tgccccatac
    16921 taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa agtagtaaga
    16981 aggcgcagtg ccaggtaatc cttttaagtc agtaatctca agaccggcag ctttatgttg
    17041 ctcgtgtaat ttttctagat catcagctgc tatagcgata tgaccatagc cgtctcctag
    17101 atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat ccccaggaag
    17161 tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc tttcttcaaa
    17221 accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcactc gaacacatgt
    17281 gtgagccatt ttcatagatt catctctcct tttcctttta ctcaatcata acaaaaagaa
    17341 gtaaggaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa atggatagaa
    17401 aataaaactg ttaaggtgga aactattcat ggataaacca atttttggtg aaaaaaagca
    17461 atttgctgat tatcaaaccc gttatgcagc atatattgtc atagaaagag atcagcagat
    17521 tgcagtaatc gaggctccaa atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga
    17581 agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga
    17641 aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc gtgcaactta
    17701 ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag gagaaccaac
    17761 tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg
    17821 tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc
    17881 gagtagatca gctttctctc agttttttta tttattacag tacatatttt ttgattgctt
    17941 cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct ttgacaagag
    18001 aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat aagtcgttct
    18061 cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt gcgagattcg
    18121 caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag ggcgcacttt
    18181 tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa
    18241 tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc atttcttcag
    18301 gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta taagcgctga
    18361 tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga ctgccgacac
    18421 ggcgtgccat cacttcgatc tctaggaaat cttcatgtgt cagtccgtga cgggcgatga
    18481 ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg taatcattat
    18541 cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga cggccagtgc
    18601 ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt acttcttttg
    18661 ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact agtttgatag
    18721 acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta cggatatggc
    18781 tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag gacggatcaa
    18841 ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa
    18901 caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa atctcgattt
    18961 gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggtagatcg tagctagaat
    19021 tcacagcagt gtaataaaca ggattatatc cgaccttttc tactaacaaa gtgagttctt
    19081 ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt ttacgattca
    19141 aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat tgagtaaagt
    19201 aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag cttccgtcaa
    19261 gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct tgatacaact
    19321 cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat accacgggaa acaggatgga
    19381 agtacacttg cacatacatt tgatatcggc taacgatata atcttccact gcatgcatgc
    19441 cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat attcgggtaa
    19501 gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa tagtccattc
    19561 gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg taagtttttt
    19621 ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga atctgataca
    19681 cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc gtatggaaga
    19741 tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt gccgcacata
    19801 aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct tttgaaaaat
    19861 ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat gaatgagtaa
    19921 aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc
    19981 gtagacgttg gacttctttc gaattgatca aatctaagat tacttgatgc tggacatgga
    20041 tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga tatttataag
    20101 gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg gatttggttg
    20161 ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg taaatcactt
    20221 gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt tacaaaaagg
    20281 ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc aggatagcca
    20341 tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac ttgcccgcgt
    20401 ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt tttgatccga
    20461 cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt tccaggacaa
    20521 taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat
    20581 aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact ttgggggaaa
    20641 atcaaagaaa tattcaaaat tccttcatct gatacgatcc ctaatccacc tgaatttctg
    20701 atcactggag cgtaacctgc atctttcagt gtttggatcc cgtcacttag aaaaggagtt
    20761 cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg taaaaagatt
    20821 tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg
    20881 tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc ggctcctttg
    20941 tataaattca tattttctac aatattatac tctaaaatca aagagttgtt ttggaaaaaa
    21001 ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg aacaatgtag
    21061 ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg atttcaatca
    21121 aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt gatttgaaag
    21181 ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt caggaggaga
    21241 cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta cagctgatcc
    21301 gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta gaaacatcct
    21361 atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg catgttagtt
    21421 tgaaagaccg tcccacagga gggaaggttc gaatcgatta tgatctgttc atgggaccgg
    21481 aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa aagaaaagtt
    21541 tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac gtgtcctgat
    21601 tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac
    21661 acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct aaccagatcc
    21721 aaaactatct tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa ttttatactg
    21781 acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc ttgcgttcat
    21841 ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa gaggatgaag
    21901 atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg
    21961 cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa gatgacgatg
    22021 atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat tctgacttgc
    22081 aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga aatatcgaag
    22141 aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag gaagaatttt
    22201 ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag agacgatcag
    22261 cctatcagaa taaggaggtg tgacacgatt tttgtcacac cttctttttt gtatataaaa
    22321 atcccaagta aatacataaa aaataaaaaa attgaaactt ttagcgcacc ttgtacgtca
    22381 tattaagtga ggcagaaagg agtggagaat gatttttact ggatataaga aaaaacggca
    22441 gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa actgattatc aaaaaatgtt
    22501 ccgtatcacg atgaattacg tacatgataa agaagaggcc ttagatgtga tgcaagatag
    22561 ttttcataaa gccctgaact attttgaaaa agaaaaagag atcgagcatt ttagtgcatg
    22621 gttttatcgg atattgatcc gcacggcatt agatagttgg cgcagacaaa aaaggacgcc
    22681 tgtggactta tttgaaattc aagatatccc ccaacagtta cctttagaaa cttccgttgc
    22741 agagctccat atgattttag ataaaataga cagtccggaa aaagagattc tgatcctcta
    22801 cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc ttggatatga atgaaaacac
    22861 agttaaaacg aaaatgtacc gctcattgaa tgctttacgt caaatcctag aatgaaggag
    22921 agaagagtgc catgtattcc caaaaagaaa atttaaaaaa actaaagaaa acttataaag
    22981 aacaagcaat tcccaaaggg cttaaagagg agatccgcca gcgttttatt atcgaagagc
    23041 aatcatttat aaaagcaaag cggagaaaga gacgtctcca agggttcgga gtatctttgg
    23101 ctatcgtaat gattacaaca agtagccttc tttttaatag tcaagttcga agctttgcag
    23161 aagatttacc catcttagga tcagtcattg aattgatctt aggcgaacgc tttactgatc
    23221 gatctgaaaa aatcgatatt caggtaccga agatcaatac acaaaatgag aaagaaaata
    23281 aaacaattca tggattaaat caaaaatact ttcgcgaagg acaagcagat tttgaaaaag
    23341 ctgaaaaaga atatggaaat tttgaaacgg atcattacca agttttggga gattatcaga
    23401 aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca gatcacccaa acagcagcag
    23461 atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc cagctctcac
    23521 ttccactttt attcaaagat gacacctatc tatctgtttt gacaaaagaa gtcaaacggc
    23581 agatggccga acaagtcaag gaagatccat ctaagtacta ttggactgag caagatattc
    23641 aagaagggac gatagaaaaa ccaacacttg ttacgcctac acgaagtttt tatttgaaca
    23701 aagagcatca attagttttg actttttctc aatacgaaat tgctcctggt tatatgggta
    23761 caccagaatt tgtcattcct aagtccgtga caaaaacgat tttggcatcg gaagattatt
    23821 tggatcatta gacgagaagt agttgcgact agcagtttca attttcaagt aagtacaagt
    23881 aaataataag aagagaaaaa gagaaagaag agatatagtg aaaagaaatt atttagttat
    23941 cagtattatt gctttatccg tgcttttgtt tttcgaaatt acttcggtgg cctccttgat
    24001 caccgaagta aaaggacaaa cacactcttc ggagcattcg tcacaatctt tcaaacaatc
    24061 agaacaatca accattaaaa gccaatcaac tgaaacagct aacgtcaaag aagccattca
    24121 agaatcagcg gaaaaggtac aaagtcagtc atcacaatca acacaagaca gtacaaaaac
    24181 gagccgagat ccggctgtca aaagcgtcgc aatcagcttt gatgatggac caggagcgac
    24241 cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca cttttttcgt
    24301 cttaggagaa aatacggcgc agcatcctga aatcgtgaag cagacagctg aagcagggca
    24361 cgagattggc aattacacat acgatcatca gaatctggcc actctttctg cccagtcgat
    24421 gacggaggaa gtgacgaaag cagacactga gatcaaaaaa gtcactggca aaacccccac
    24481 ttttgttcgt ccgccctatg gttctgtcac gaatgtagga gcaacgatca tccagcgacc
    24541 gatcatcgaa tggtctgttg attcagaaga ttggaagaca agaaatcctg atttgatttt
    24601 gcagaagatc caagcaacag tatacgatgg ggcaattatt ttattccatg atatctatcc
    24661 tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg aaagagcaag ggtatcgcat
    24721 cacgacagtt ggggatttat tagggcatcc aacggctgtc gagaactatt atggcagaaa
    24781 cgatcatcgg ccagttcagt aagtgagaca agtatcttta ataaataaca cattttaata
    24841 agtagttgaa cacataacta ccttacttgc aaaggcttaa caatatctat acaggtacaa
    24901 caaaaaactg tccaccaatt ttctttggcg gacagttttc acttaaaaat aaagaagcaa
    24961 ctattcgtaa attatgctaa aatagcagta gaacgcgtcc tgagggaatc gaacccccgt
    25021 ctcaagaacc ggaatcttac gtgatatcca ctacactaag gacgcaagca ctgggataat
    25081 tgtaactaaa cagataaaaa attacaaggg gattggacaa aaaaatgaaa tttgagcaaa
    25141 atttttcaca aaaacagcag cagacacaga aactagccat gacgcaacag ttgcaacagt
    25201 ccatccaagt tttacaatat aacagtgagg agctgttggc attcgttgag aatcaagcaa
    25261 tggaaaatcc attggtggaa gttgttgagc cggaatggca gccagattac ataaaagctt
    25321 cttcatcttc ttatgaagga gaagagacaa attacctaaa tcagattcct gatacaaaag
    25381 ggtctttatt tgattcattg atcgaacaag tccatttgaa ttaccgagat acttttttga
    25441 ggaaaatcgt tctttatctt gtagaataca tcgatttgaa cggatttttg acgattagtt
    25501 tagaagaagc cggaaaagag attggagcta cgcctatcca aatgctcgat gcgttgactc
    25561 tgattcagca attagaacca gctggagtag gtgcacgtag tttgcaagaa tgtctcatgc
    25621 ttcaaacaga acgagatgat tatgcgccgg aattagctta tatcgtgttg gaagaatgtt
    25681 ttgaggaact ggtggagcga aaatggaaag aaatcgcaca acgtttttct gttgatctac
    25741 atgctgttca gcagatattc gactatttac aaacgttgag tccaagccct ggccggattt
    25801 tcgaccgtag cagcgaattg tacattattc ctgatgtccg ggtattagtg gacgacgata
    25861 aaaatgtaca agttatctca aaccgaaaaa atcagccaaa tatacgcttt caagagagtt
    25921 acttcaaaca aatgtcacaa caagcagata aagaaactga aaattattta aaagaacgga
    25981 aacaggaatt cgaatggttg aagaaaacga tcctacaacg tggggataca attttgcggg
    26041 ttgctcaagt cattgtttcc cggcaaaaag ctttctttat cgacaaggaa cgtccaatca
    26101 agcctcttac gttaaaggaa gttgcaactg agatagatgt gcatgaatcc acggtcagtc
    26161 gtgcagtcaa cgggaaatat ttggaaacag atttcggtgt atttgaatta aaaaaatttt
    26221 tcactacacg aattcctacg aatagtacag aacagacaga agacctttca gcagatacag
    26281 cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa aaacaagccg ttgtcggacc
    26341 agaaattggt tgaattgctg aaaaaagatg agatagcgat ctcaagacga acagtagcaa
    26401 aatatcgtga tttattagga atcccgagtt catcaaaaag aaaacgttat gataactaat
    26461 cagtcaaaag actagaaaaa gtatttcact ttttctagtc tttttagttg aaaaacgaga
    26521 gcgaaactgt tagacttaca gtcgtgaggt tgagatttcc ctttattgat gtgaaattcg
    26581 aatagttaat ttcttttttt atcgtatatg ggtcgtttta agtctacatt ggacgtaaag
    26641 tgtccaacta aggagatcgc tatgctggat gaaataaaaa tgattgaatc tgttgcacca
    26701 gatattatcg aagtgttgca agaacgctat aaaattttac gaaatatcta ttggatgcag
    26761 ccgattgggc gtcgaagttt atcggaaagt atggggatca cagaacgagt cttacgaaca
    26821 gaaacagacc ttttgaaaaa tctgaatttg atcgacacct caaaaagcgg tatgacgctg
    26881 acaactaagg gcgaagaagt ttatcaaagc cttgaaaact tcatggatca gcttttaggt
    26941 acgcatcaaa ccgaacaaca gttggcagaa tattttggta accaacgctg tatcgttgtt
    27001 tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat tcggcgaagc gctgagcgaa
    27061 gccttagacc gactacttcc agaaggcgag aatatcatcg ctgtgatggg aggaactact
    27121 atggctgtgg tagccgaaca attgtctaat ctggaaaata agaaacggca taacctgttt
    27181 gtccctgcaa gaggcgggat tggtgaagcg ataactgtac aggcaaattc tgtcagtgca
    27241 agaatggctg ctaaagcaaa cggtaaccac cgcgcacttt atgttccgga acaattaagt
    27301 ctggcaactt acaattcttt actcaatgag ccatctatcc aagaagtctt aaacttgatt
    27361 agtgaagcga attgtgttat tcatagtatt gggcgtgcat tacacatggc agcacgtcgt
    27421 aagatgactg aaaaagaatt agtcatgctg aaacaagcaa atgctgttgc ggaatctttt
    27481 ggctactttt tcaatgaaaa aggtgaagtc gtttataagg tccctagaat cggcatagaa
    27541 ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg cgggcggaaa atcaaaagct
    27601 aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa cgtggctcat cactgatgaa
    27661 gctgctgcaa atgagatttt aaaaggggta accctttaaa ataaatattt ttttgatttt
    27721 cataaggagg aaatcttaaa tgacagttaa agtaggtatt aatggttttg gacgtattgg
    27781 acgtctagca ttccgtcgta tccaagatgt agatggaatc gaagtagtag caatcaacga
    27841 cttgacagat gctaaaatgt tggcacactt gttaaaatac gacacaactc aaggacgttt
    27901 caacggaaca gttgaagttc atgaaggttc attcaacgta aacggaaaag aagttaaagt
    27961 attagctaac cgcaacccag aagaattacc atggggcgaa ctaggcgtag atatcgttct
    28021 agaatgtact ggtttcttca cttctaaatc agcagctgaa aaacatttaa cagctggtgc
    28081 taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt ccaacaatcg tttataacac
    28141 aaaccacgaa acattaactg gtaaagaaac agttatctca ggtgcttcat gtactactaa
    28201 ctgtttagct cctatggcta aaacattgaa cgacaaattt ggtgttgttg aaggattaat
    28261 gacaactatc cacgcttaca caggtgacca aatgactcta gacggacctc atcctaaagg
    28321 tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt cctaactcaa caggtgctgc
    28381 taaagctatc ggtttagtaa ttcccgaatt gaacggtaaa ttagacggag ctgctcaacg
    28441 tgttcctgta ccaacaggtt cattaacaga attagtaaca gttcttgaaa aagaagtaac
    28501 tgttgacgaa atcaatgcag caatgaaaga agcttcaaac gaatcttacg gatacaacac
    28561 agacgaaatc gtttcttcag atatcgttgg tatgacttac ggttcattat ttgatgctac
    28621 acaaactaaa gtaatgacag ttggcgacaa acaattagtt aaaactgttg cttggtacga
    28681 caacgaaatg tcatacactg cacaattagt acgtacttta gaatacttcg ctaacttata
    28741 agatagacaa acgtacaagt gtaaattata gacatgtaca tttaaaagcg gggaagcaat
    28801 cgcgcttctt ccgctttttt tgataataag tttattcagg aggaaataaa atggctaaga
    28861 aaacagtgaa agatatcgat ttaaaagaca aaaaagttct tgtccgtgtt gacttcaacg
    28921 ttcctttgaa agacggcgtg atcactgacg acactcgtat caaagcagca ttgccaacaa
    28981 tcaactacgt tctagaacaa ggcggaaaag caatcctttt ctctcacctt ggacgtgtaa
    29041 aaacagaaga agataaagaa ggaaaatctt tagctccagt tgcaaaacgt ctaggcgaat
    29101 tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg agaacaatta gaagaagcaa
    29161 tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa tacacgattt gaagatattg
    29221 acggtaaaaa agaaagcaaa aatgatacag aactaggtaa atactgggct tcattaggcg
    29281 atgtatttgt taacgatgct ttcggtacag ctcaccgtgc acatgcttct aacgtaggta
    29341 tcgcttcaac tggtatccca acagttgctg gattcttgat ggaaaaagaa atcaagttca
    29401 tcggtgaagc agtagaagaa ccaaaacgac caatgattgc aatccttggt ggcgcaaaag
    29461 tttctgataa aatcggcgta atcgaaaact tgattcctaa agcagataaa atcttgatcg
    29521 gtggcggaat gacttacaca ttctacgaag caaaaggaat caagatcggt aactctctag
    29581 tagaagctga taaagtggaa ctagcaaaag aattaatcga aaaagcaggc gacaaacttg
    29641 tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa tgatgttgaa acacaagtaa
    29701 tcgaaggaga cattcctgat ggtttgatgg ctttagacat cggcccagca tcagtaaaat
    29761 tatttgctga tacattacaa ggtgcaaaaa cagttgtttg gaacggacct atgggtgtat
    29821 ttgaaatgag taactttgct aaaggtacaa tcggtgtttg tgaagcaatc gctaacttag
    29881 aaggcgcaac aacaatcatc ggtggcggag actcagccgc agcagcagaa caattaggat
    29941 ttgctgacaa attcacacac atctcaacag gtggcggcgc aagcttggaa ttgcttgaag
    30001 gtaaagaatt accaggacta gcagcaatca acgataaata aacgaccaag tttgctcaat
    30061 tctgacgagt aatcgaacaa aaggtgaagg gagttttcat gactccctag ccatttttca
    30121 ataaattaag gagtgtgttc ctcaatgcgt aaacctatta tcgcaggtaa ctggaaaatg
    30181 aacaaaactg catcagaagc aaaagaattt gctgaagcag taaaaaacaa aatcccttca
    30241 aatgacaaag ttgattcagt catcggatct cctgcattat tcttacaaga attaatgaca
    30301 tcagctgaag gtacagaact aaaaatctca gcacaaaact gctactggga aaactcaggt
    30361 gcgttcactg gtgaaacttc accagcagct cttgctgatc taggtgttca ttatgtgatc
    30421 atcggacatt cagaacgtcg tgaatatttc catgaaacag acgaagacat caataaaaaa
    30481 gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat gctgcggcga atctttagaa
    30541 acctatgaag caggcaaaac tgctgaatgg atcgaaggac aaattacagc tggtttagct
    30601 gatctttcag ctgaacaggt gtcaaacatg gttattgctt atgaaccaat ctgggcaatc
    30661 ggtactggta aatcagctga cgcacaaatc gctgacgaaa tttgtggagt tgtacgtcaa
    30721 acagttgaaa aactttatgg aaaagaagta tcagaagcag ttcgtatcca atacggcggt
    30781 tctgtaaaac cagaaaatat tgctgaatac atggcaaaag aaaatgttga cggtgcttta
    30841 gtcggtggcg caagcttgca agcagattca ttcttgtcat tgttagacgc tgtaaaataa
    30901 ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact aatgagcaaa attaactaaa
    30961 atcgtatatg agggaaatcc cttaggaaaa aacaaactca aaggagagac aaaacatgtc
    31021 aattattact gatgtttacg ctcgcgagat cttagactca cgtggtaacc caacaatcga
    31081 agtagaagta tacacagaaa gcggagcttt tggccgcgga atggttcctt caggtgcttc
    31141 aactggtgaa tacgaagcag ttgaattacg tgacggagac aaagctcgtt atggcggtaa
    31201 aggtgtaact aaagctgtcg acaacgtaaa caacatcatt gctgaagcaa tcattggcta
    31261 cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc gctttagacg gaactcctaa
    31321 caaaggtaaa ttaggtgcga acgctatttt aggtgtttct atcgctgttg cacgtgcagc
    31381 tgctgactac ctagaagtac ctttatacca ctacctaggc ggattcaaca caaaagtatt
    31441 gccaacacca atgatgaaca tcatcaacgg cggatcacat gctgataact caatcgactt
    31501 ccaagaattc atgatcatgc ctgttggcgc tccaacattc aaagaagctc tacgtatggg
    31561 tgctgaagta ttccacgcat tagcttcaat cttgaaagct cgcggattag caacttctgt
    31621 tggtgacgaa ggtggatttg ctccaaacct tggttcaaac gaagaaggtt ttgaagtaat
    31681 catcgaagca atcgaaaaag ctggctatgt acctggtaaa gacgttgttc ttgctatgga
    31741 tgctgcttct tcagaattct acgacaaaga aaaaggtgtt tacgtactag ctgactcagg
    31801 cgaaggcgaa aaaacaactg acgaaatgat caaattctac gaagaattag tttctaaata
    31861 tccaatcatc tcaatcgaag atggattaga cgaaaacgac tgggacggat tcaagaaatt
    31921 aactgacgta ttaggcgaca aagttcaatt agttggtgac gacttgttcg taacaaacac
    31981 tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac tcaatcctta tcaaagtgaa
    32041 ccaaatcggt acattaacag aaactttcga agctatcgaa atggctaaag aagctggcta
    32101 cacagcagtt gtatctcacc gttctggtga aacagaagat tcaacaatct ctgatatcgc
    32161 tgttgcaaca aacgctggcc aaatcaaaac tggttctcta tcacgtactg accgtattgc
    32221 taaatacaac caattactac gtatcgaaga ccaacttggt gaagttgcag aatacaaagg
    32281 tttgaaatct ttctacaact taaaaaacaa ataattcttt tctaatgaag atacatgatc
    32341 gactattgaa agatagtcga gtatgatcac ttagttaagt aatcgaactc tctacttgct
    32401 gaaata
//
LOCUS       DACECJ010000025        32176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.025, whole genome shotgun sequence.
ACCESSION   DACECJ010000025 DACECJ010000000
VERSION     DACECJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.025"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(24..203)
                     /locus_tag="IWO09_001200"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="IWO09_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="IWO09_001201"
     CDS             complement(407..766)
                     /locus_tag="IWO09_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0263807.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="IWO09_001202"
     CDS             complement(796..1137)
                     /locus_tag="IWO09_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263808.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="IWO09_001203"
     CDS             complement(1134..1808)
                     /locus_tag="IWO09_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0263809.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IWO09_001204"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IWO09_001204"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0263810.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="IWO09_001205"
     CDS             complement(4322..5512)
                     /locus_tag="IWO09_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0263811.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="IWO09_001206"
     CDS             complement(5533..6060)
                     /locus_tag="IWO09_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0263812.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="IWO09_001207"
     CDS             complement(6107..8896)
                     /locus_tag="IWO09_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0263813.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="IWO09_001208"
     CDS             complement(9043..9243)
                     /locus_tag="IWO09_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0263814.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="IWO09_001209"
     CDS             complement(9696..10016)
                     /locus_tag="IWO09_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263815.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="IWO09_001210"
     CDS             complement(10519..11637)
                     /locus_tag="IWO09_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263816.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="IWO09_001211"
     CDS             12108..13415
                     /locus_tag="IWO09_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0263817.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="IWO09_001212"
     CDS             13536..14735
                     /locus_tag="IWO09_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0263818.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="IWO09_001213"
     CDS             complement(14762..16030)
                     /locus_tag="IWO09_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0263819.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="IWO09_001214"
     CDS             complement(16200..16841)
                     /locus_tag="IWO09_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ0263820.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="IWO09_001215"
     CDS             complement(17250..17753)
                     /locus_tag="IWO09_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0263821.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="IWO09_001216"
     CDS             complement(17876..18640)
                     /locus_tag="IWO09_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0263822.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="IWO09_001217"
     CDS             18748..19023
                     /locus_tag="IWO09_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0263823.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="IWO09_001218"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="IWO09_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0263824.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="IWO09_001219"
     CDS             complement(20579..20980)
                     /locus_tag="IWO09_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0263825.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="IWO09_001220"
     CDS             complement(20994..21803)
                     /locus_tag="IWO09_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0263826.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="IWO09_001221"
     CDS             complement(21790..22680)
                     /locus_tag="IWO09_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0263827.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="IWO09_001222"
     CDS             complement(22693..23172)
                     /locus_tag="IWO09_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0263828.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(23348..24289)
                     /locus_tag="IWO09_001223"
     CDS             complement(23348..24289)
                     /locus_tag="IWO09_001223"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAQ0263829.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IWO09_001224"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IWO09_001224"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0263830.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="IWO09_001225"
     CDS             complement(25274..26470)
                     /locus_tag="IWO09_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0263831.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="IWO09_001226"
     CDS             complement(26488..27216)
                     /locus_tag="IWO09_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0263832.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IWO09_001227"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IWO09_001227"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0263833.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="IWO09_001228"
     CDS             complement(28628..29086)
                     /locus_tag="IWO09_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0263834.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="IWO09_001229"
     CDS             complement(29083..30546)
                     /locus_tag="IWO09_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0263835.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IWO09_001230"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IWO09_001230"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0263836.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32176
                     /locus_tag="IWO09_001231"
     CDS             31602..>32176
                     /locus_tag="IWO09_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0263837.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFF"
BASE COUNT         9384 a         6920 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcaacaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttgtcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaa
//
LOCUS       DACECJ010000026        32112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.026, whole genome shotgun sequence.
ACCESSION   DACECJ010000026 DACECJ010000000
VERSION     DACECJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.026"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IWO09_001232"
     CDS             complement(27..725)
                     /locus_tag="IWO09_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0263838.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IWO09_001233"
     CDS             complement(718..2070)
                     /locus_tag="IWO09_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0263839.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IWO09_001234"
     CDS             complement(2293..3693)
                     /locus_tag="IWO09_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0263840.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IWO09_001235"
     CDS             3854..4591
                     /locus_tag="IWO09_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0263841.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IWO09_001236"
     CDS             4716..5195
                     /locus_tag="IWO09_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0263842.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IWO09_001237"
     CDS             5192..5959
                     /locus_tag="IWO09_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263843.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IWO09_001238"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IWO09_001238"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0263844.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IWO09_001239"
     CDS             complement(6862..7464)
                     /locus_tag="IWO09_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0263845.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IWO09_001240"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IWO09_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IWO09_001241"
     CDS             8145..8729
                     /locus_tag="IWO09_001241"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0263846.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IWO09_001242"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IWO09_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0263847.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IWO09_001243"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IWO09_001243"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0263848.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IWO09_001244"
     CDS             10630..10809
                     /locus_tag="IWO09_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263849.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IWO09_001245"
     CDS             10831..11856
                     /locus_tag="IWO09_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0263850.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IWO09_001246"
     CDS             12031..13275
                     /locus_tag="IWO09_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0263851.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="IWO09_001247"
     CDS             13429..14376
                     /locus_tag="IWO09_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0263852.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="IWO09_001248"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="IWO09_001248"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0263853.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="IWO09_001249"
     CDS             complement(15278..15520)
                     /locus_tag="IWO09_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263854.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="IWO09_001250"
     tRNA            15641..15713
                     /locus_tag="IWO09_001250"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="IWO09_001251"
     CDS             complement(15832..16470)
                     /locus_tag="IWO09_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0263855.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="IWO09_001252"
     CDS             complement(16593..17225)
                     /locus_tag="IWO09_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0263856.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="IWO09_001253"
     CDS             17576..17875
                     /locus_tag="IWO09_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0263857.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18370..20535
                     /gene="acm"
                     /locus_tag="IWO09_001254"
     CDS             18370..20535
                     /gene="acm"
                     /locus_tag="IWO09_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0263858.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20807..21292)
                     /locus_tag="IWO09_001255"
     CDS             complement(20807..21292)
                     /locus_tag="IWO09_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0263859.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21772..22413
                     /locus_tag="IWO09_001256"
     CDS             21772..22413
                     /locus_tag="IWO09_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0263860.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22432..23067
                     /locus_tag="IWO09_001257"
     CDS             22432..23067
                     /locus_tag="IWO09_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263861.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23085..23900
                     /locus_tag="IWO09_001258"
     CDS             23085..23900
                     /locus_tag="IWO09_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0263862.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24219..25655
                     /locus_tag="IWO09_001259"
     CDS             24219..25655
                     /locus_tag="IWO09_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0263863.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25844..28291
                     /locus_tag="IWO09_001260"
     CDS             25844..28291
                     /locus_tag="IWO09_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0263864.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            28521..29588
                     /gene="rlmN"
                     /locus_tag="IWO09_001261"
     CDS             28521..29588
                     /gene="rlmN"
                     /locus_tag="IWO09_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0263865.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(29781..30884)
                     /locus_tag="IWO09_001262"
     CDS             complement(29781..30884)
                     /locus_tag="IWO09_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0263866.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            31437..31985
                     /gene="aac(6')"
                     /locus_tag="IWO09_001263"
     CDS             31437..31985
                     /gene="aac(6')"
                     /locus_tag="IWO09_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0263867.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        11023 a         5470 c         6524 g         9095 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaaat gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa
    18181 taatttacat taggaaaaac cttgtaaaaa cagttttttg gcatatttat tttttataaa
    18241 tatttgtaaa ataactatca attttttagt taaaagattg gaaatgagtg ggggaggaat
    18301 tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa aataggggag
    18361 atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat tattactaat
    18421 ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat cagcagtaat
    18481 gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat aaaagtaaaa
    18541 ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg gatcaattgg
    18601 ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt aatgattgaa
    18661 aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt gtttaatgaa
    18721 aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca ggccagaaac
    18781 gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg tgataaaaca
    18841 gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt ttattataaa
    18901 acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa tattaacaat
    18961 aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag tggacaaaga
    19021 ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa ggtttataga
    19081 ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt taatttttca
    19141 gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct taggaaaata
    19201 atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga gaatcattcg
    19261 gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt caatcataca
    19321 gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga attgaagatt
    19381 ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga attaagacga
    19441 gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa tgaacaggga
    19501 gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa agaagcaccc
    19561 aattggattg attttgatcc actaagttca aatgaactca aattctccat aaacgaaaat
    19621 gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa catcacagca
    19681 actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa attattcaga
    19741 gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt agacaatgga
    19801 ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa cgaatatacg
    19861 tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa
    19921 attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt ctcaggacaa
    19981 aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat cacagtaaat
    20041 ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc
    20101 tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta tactgtaacg
    20161 gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa aaacagttat
    20221 acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg gaaaaatttt
    20281 ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt gcaaatattt
    20341 gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag agctgtacct
    20401 aaacagataa caaataaatc aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt
    20461 tttgctgtaa tcattggttt attgatagta actatctgtg ctgggatatt cttacaaaaa
    20521 tataaaaaag tttagttaga atataatatt ttttgttaaa tatgactgaa atcaaaagaa
    20581 taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag
    20641 aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc
    20701 atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat
    20761 aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat
    20821 aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca
    20881 acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat
    20941 cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac
    21001 ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt
    21061 cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa
    21121 gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta
    21181 gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga
    21241 tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc
    21301 accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt
    21361 aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt
    21421 tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc
    21481 gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct
    21541 agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt
    21601 gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga
    21661 ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt
    21721 cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat
    21781 ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa attattttta
    21841 tttaccttga ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat
    21901 tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg
    21961 ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt
    22021 ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt
    22081 cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta
    22141 agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct
    22201 tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt
    22261 ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct
    22321 ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat cataatgaaa
    22381 caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa
    22441 ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc
    22501 attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt
    22561 ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt agacggtgta
    22621 ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac
    22681 ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta
    22741 ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg
    22801 attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta
    22861 gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt
    22921 gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat
    22981 ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa
    23041 ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt
    23101 ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt
    23161 cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata
    23221 catttgttcc tatgggattt agagataaag acgggaagct cacaggattt gatattgatt
    23281 tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt
    23341 caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca
    23401 aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag
    23461 tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat
    23521 taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa
    23581 aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg
    23641 atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt
    23701 tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg
    23761 attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc
    23821 tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag
    23881 atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt
    23941 tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct
    24001 cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg
    24061 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
    24121 ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa
    24181 agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct
    24241 catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt
    24301 ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga
    24361 catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata
    24421 ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga
    24481 tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga
    24541 tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc
    24601 cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca
    24661 ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt
    24721 tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg
    24781 tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg
    24841 tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata
    24901 cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag
    24961 acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca
    25021 tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc
    25081 tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat
    25141 tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat
    25201 cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa
    25261 caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa
    25321 gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga
    25381 cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag
    25441 ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata
    25501 cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc
    25561 aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat tactggtacc
    25621 aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta
    25681 cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta
    25741 aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta
    25801 cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg
    25861 gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca
    25921 aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa
    25981 attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta
    26041 ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga
    26101 gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt
    26161 attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg
    26221 gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg
    26281 tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg
    26341 aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga
    26401 aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga
    26461 aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg
    26521 cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc
    26581 agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca
    26641 ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga
    26701 aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac
    26761 agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat
    26821 tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat
    26881 accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga
    26941 acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc
    27001 cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt
    27061 aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc
    27121 aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag
    27181 agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg
    27241 tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc
    27301 tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat
    27361 gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa
    27421 tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc
    27481 gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac
    27541 cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg
    27601 aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat
    27661 gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc
    27721 agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat
    27781 catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg
    27841 gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt
    27901 gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt
    27961 agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat
    28021 gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga
    28081 agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag
    28141 atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt
    28201 tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc
    28261 agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga
    28321 ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct
    28381 tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga
    28441 tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg
    28501 agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat
    28561 taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat
    28621 ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga
    28681 tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag
    28741 cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg
    28801 tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata
    28861 tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag
    28921 aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac
    28981 gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa
    29041 tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta
    29101 cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag
    29161 tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga
    29221 tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa
    29281 caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc
    29341 atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga
    29401 ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat
    29461 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
    29521 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
    29581 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
    29641 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
    29701 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
    29761 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
    29821 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
    29881 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
    29941 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
    30001 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
    30061 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
    30121 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
    30181 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
    30241 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
    30301 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
    30361 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
    30421 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
    30481 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
    30541 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
    30601 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
    30661 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
    30721 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
    30781 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
    30841 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
    30901 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
    30961 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
    31021 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
    31081 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
    31141 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
    31201 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
    31261 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa
    31321 gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga
    31381 acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga
    31441 taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga
    31501 gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga
    31561 atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg
    31621 caatccctca atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc
    31681 gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca
    31741 gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc
    31801 aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac
    31861 atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa
    31921 atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc
    31981 aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg
    32041 aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga
    32101 acaccgtgta cc
//
LOCUS       DACECJ010000027        31839 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.027, whole genome shotgun sequence.
ACCESSION   DACECJ010000027 DACECJ010000000
VERSION     DACECJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.027"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(151..2304)
                     /locus_tag="IWO09_001264"
     CDS             complement(151..2304)
                     /locus_tag="IWO09_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0263868.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="IWO09_001265"
     CDS             complement(2598..3446)
                     /locus_tag="IWO09_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0263869.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWO09_001266"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWO09_001266"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0263870.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="IWO09_001267"
     CDS             complement(5947..6126)
                     /locus_tag="IWO09_001267"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0263871.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWO09_001268"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWO09_001268"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0263872.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="IWO09_001269"
     CDS             8274..10553
                     /locus_tag="IWO09_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0263873.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="IWO09_001270"
     CDS             complement(10646..11293)
                     /locus_tag="IWO09_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263874.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="IWO09_001271"
     CDS             complement(11472..12191)
                     /locus_tag="IWO09_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0263875.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="IWO09_001272"
     CDS             complement(12322..13473)
                     /locus_tag="IWO09_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0263876.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="IWO09_001273"
     CDS             complement(13690..14430)
                     /locus_tag="IWO09_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0263877.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWO09_001274"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWO09_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0263878.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /locus_tag="IWO09_001275"
     CDS             complement(14814..15407)
                     /locus_tag="IWO09_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0263879.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16038)
                     /locus_tag="IWO09_001276"
     CDS             complement(15391..16038)
                     /locus_tag="IWO09_001276"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0263880.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWO09_001277"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWO09_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0263881.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWO09_001278"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWO09_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0263882.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="IWO09_001279"
     CDS             complement(17484..18011)
                     /locus_tag="IWO09_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0263883.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="IWO09_001280"
     CDS             complement(18191..18976)
                     /locus_tag="IWO09_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0263884.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="IWO09_001281"
     CDS             complement(18969..19838)
                     /locus_tag="IWO09_001281"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0263885.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="IWO09_001282"
     CDS             complement(19839..21218)
                     /locus_tag="IWO09_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0263886.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWO09_001283"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWO09_001283"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0263887.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWO09_001284"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWO09_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0263888.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWO09_001285"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWO09_001285"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0263889.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWO09_001286"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWO09_001286"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0263890.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWO09_001287"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWO09_001287"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0263891.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="IWO09_001288"
     CDS             complement(25176..25400)
                     /locus_tag="IWO09_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0263892.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="IWO09_001289"
     CDS             complement(25453..26415)
                     /locus_tag="IWO09_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0263893.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="IWO09_001290"
     CDS             complement(26425..26865)
                     /locus_tag="IWO09_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0263894.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="IWO09_001291"
     CDS             27171..28136
                     /locus_tag="IWO09_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0263895.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="IWO09_001292"
     CDS             complement(28193..28423)
                     /locus_tag="IWO09_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0263896.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="IWO09_001293"
     CDS             complement(28509..29396)
                     /locus_tag="IWO09_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0263897.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="IWO09_001294"
     CDS             complement(29485..30708)
                     /locus_tag="IWO09_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0263898.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31804)
                     /locus_tag="IWO09_001295"
     CDS             complement(30725..31804)
                     /locus_tag="IWO09_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0263899.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8794 a         6970 c         5488 g        10587 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctatt
//
LOCUS       DACECJ010000028        28043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.028, whole genome shotgun sequence.
ACCESSION   DACECJ010000028 DACECJ010000000
VERSION     DACECJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.028"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWO09_001296"
     CDS             <1..395
                     /locus_tag="IWO09_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263900.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IWO09_001297"
     CDS             398..1228
                     /locus_tag="IWO09_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0263901.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IWO09_001298"
     CDS             1244..2095
                     /locus_tag="IWO09_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0263902.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IWO09_001299"
     CDS             2092..2451
                     /locus_tag="IWO09_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0263903.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IWO09_001300"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IWO09_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IWO09_001301"
     CDS             2624..2929
                     /locus_tag="IWO09_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263904.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IWO09_001302"
     CDS             2929..3240
                     /locus_tag="IWO09_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263905.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IWO09_001303"
     CDS             3407..3823
                     /locus_tag="IWO09_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0263906.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IWO09_001304"
     CDS             3823..4491
                     /locus_tag="IWO09_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0263907.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IWO09_001305"
     CDS             4488..4865
                     /locus_tag="IWO09_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263908.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IWO09_001306"
     CDS             4862..5161
                     /locus_tag="IWO09_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263909.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IWO09_001307"
     CDS             5403..5819
                     /locus_tag="IWO09_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0263910.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            6379..6567
                     /locus_tag="IWO09_001308"
     CDS             6379..6567
                     /locus_tag="IWO09_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263911.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            6557..6817
                     /locus_tag="IWO09_001309"
     CDS             6557..6817
                     /locus_tag="IWO09_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263912.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            6858..7085
                     /locus_tag="IWO09_001310"
     CDS             6858..7085
                     /locus_tag="IWO09_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263913.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            7150..7722
                     /locus_tag="IWO09_001311"
     CDS             7150..7722
                     /locus_tag="IWO09_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263914.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            7723..8034
                     /locus_tag="IWO09_001312"
     CDS             7723..8034
                     /locus_tag="IWO09_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0263915.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            8025..8285
                     /locus_tag="IWO09_001313"
     CDS             8025..8285
                     /locus_tag="IWO09_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263916.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            8446..8805
                     /locus_tag="IWO09_001314"
     CDS             8446..8805
                     /locus_tag="IWO09_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263917.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            8802..10445
                     /locus_tag="IWO09_001315"
     CDS             8802..10445
                     /locus_tag="IWO09_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0263918.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            10480..11562
                     /locus_tag="IWO09_001316"
     CDS             10480..11562
                     /locus_tag="IWO09_001316"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263919.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            11606..12778
                     /locus_tag="IWO09_001317"
     CDS             11606..12778
                     /locus_tag="IWO09_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0263920.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            12765..13469
                     /locus_tag="IWO09_001318"
     CDS             12765..13469
                     /locus_tag="IWO09_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0263921.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            13474..14634
                     /locus_tag="IWO09_001319"
     CDS             13474..14634
                     /locus_tag="IWO09_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0263922.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            14710..14988
                     /locus_tag="IWO09_001320"
     CDS             14710..14988
                     /locus_tag="IWO09_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0263923.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            14969..15340
                     /locus_tag="IWO09_001321"
     CDS             14969..15340
                     /locus_tag="IWO09_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0263924.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            15356..15760
                     /locus_tag="IWO09_001322"
     CDS             15356..15760
                     /locus_tag="IWO09_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263925.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            15757..16167
                     /locus_tag="IWO09_001323"
     CDS             15757..16167
                     /locus_tag="IWO09_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263926.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            16228..16815
                     /locus_tag="IWO09_001324"
     CDS             16228..16815
                     /locus_tag="IWO09_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0263927.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            16888..17355
                     /locus_tag="IWO09_001325"
     CDS             16888..17355
                     /locus_tag="IWO09_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAQ0263928.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            17401..17709
                     /locus_tag="IWO09_001326"
     CDS             17401..17709
                     /locus_tag="IWO09_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263929.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            17904..24638
                     /locus_tag="IWO09_001327"
     CDS             17904..24638
                     /locus_tag="IWO09_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0263930.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            24642..25514
                     /locus_tag="IWO09_001328"
     CDS             24642..25514
                     /locus_tag="IWO09_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263931.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            25511..26138
                     /locus_tag="IWO09_001329"
                     /pseudo
     CDS             25511..26138
                     /locus_tag="IWO09_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26099..26560
                     /locus_tag="IWO09_001330"
     CDS             26099..26560
                     /locus_tag="IWO09_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263932.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            26564..27388
                     /locus_tag="IWO09_001331"
     CDS             26564..27388
                     /locus_tag="IWO09_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263933.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            27406..27951
                     /locus_tag="IWO09_001332"
                     /pseudo
     CDS             27406..27951
                     /locus_tag="IWO09_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT        10112 a         4198 c         6061 g         7672 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 gaattttaac tccttctaaa ttgttcttat tatctatcat cagttgctgt ctattgtcat
     6061 tatgtcactg tggtggaaag gtgtatcgct catctaaaat ttaggtgcaa actgcaaggt
     6121 tcgattcctt gccagtgact ttggtaaccg aggcatcggc ggtttaaaaa tataggggtg
     6181 cgcaatttcg tacgcgtttt gtgcatcttg taggttgcta ttacataatt ggttgggtta
     6241 gattgagttt tgggattcgg tacaaatgaa tcgtcaaatg actcaagcac aggatcggaa
     6301 acgtccctgc ctgtgcatta catattagat cactctttga gtggtctttt tattttaaaa
     6361 aagagaggat tttgaaaaat ggaattgatt gtatttacta ataacggaca aacatatcat
     6421 ttttatgaag tggagaattt taagccaaca acaacaggat ttagttttat ttatactggg
     6481 aaggctacgg gagttactcg ttcagctacg tttaacaata catcaacagc tggttatgcg
     6541 cttgcggagg tacaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg
     6601 tgaaagaatt gaaaatgaga ttgctgacct aagtggagca gtgcctgagt ttagtatttt
     6661 catagtctgg tcatgcaaaa cactgcaaaa tagcaaagca ttagtcagcg ctagcttaaa
     6721 aggagcaccg tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac
     6781 ttatctcaaa aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac
     6841 ataaaggagg ctgcataatg agaaactact ggtatgtatc actaactaat gaatatcctc
     6901 gaaccattgg tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact
     6961 ctattgtcga aatgctaaga gaagctacac caaacgaagt ggataaatgc aagctgatat
     7021 attgcggtca tggctattgg aaagacgagt atatccaata caacattgag aggtacatca
     7081 aatgaatgat aactatgatt acatcaagtt gttagcaagt aattattaga tgtttttagg
     7141 aggaaaacta tgaaattaaa agattatatc agagaagggt ataacgttgt aactgcaccc
     7201 aatctagctt acaaaattca agaagattat cccaatgctt tagtgtttac tgatagagct
     7261 ttagatgcca ttcctatagg gaagttatta gtagatcgtt tttatagcaa taatccagct
     7321 gttcttagtg ctaagccact tcaaaacgct tatgctattg aacgctttac ttgcgagttt
     7381 actggatatg agacggtagt accaatgaca ggtgagacag gaagaaaagt aattcaacag
     7441 atcaaaaaga ggattgctga attgacgtta gatgatggaa gtaatacgaa ccttctagaa
     7501 atcaaactaa gagatactga ctcagtacca gaagtttatt ataaaggtca acgaatggat
     7561 gagatgccga taggtcttgt cgatattact tatcattggg atacggaaga atatgaatgc
     7621 caagtaccaa aaggagctaa cgacatcact attgaatatt atgattcaca taataacaag
     7681 tacttagata gaaaaatcat tgggcacaag agagatatgt aaatgattga tgtttcaagt
     7741 aaacaagcaa gagctaggtt ctatggatca actgagtgga ggaaacttag aagtcaatgc
     7801 ttagagcgag atcattatga gtgccaatgg tgtaaagcag agggtagact aacaactcaa
     7861 catgattcaa ttcttgaagt ggatcatata aaggagctgg agtatcatcc ggatttagct
     7921 ctagatataa acaacctaag aaccttatgc aaagattgcc ataacaaaag gcacaaccgt
     7981 atgaaatatc gtggccaaca aaagaaaaga aagtgggatg atgaatggtg gtaagaaaaa
     8041 cagcaaacag agatgatgtc aaacgagcag aagcaatcgc tatggttgat aaggctattg
     8101 ataagttggt tctatttaag agtcagctca ttgaaggaac aacaactgta gaggatatat
     8161 ctatcaacaa tagactaata ggtaacgcta atgaagtaac agggacattt aatattaatg
     8221 tagacattga ttataccttg agtccatttg cacatattgt agaaagcaac gagcaagacg
     8281 gctgataaat cagtataaag cgaagatata tagagtgaaa tacccccggt caaaagattt
     8341 tgagaagaaa taaaattttt gggaaccggt ggatggggtc aactctgcaa ataaatgatg
     8401 ctattttcac acgaccccct ccccacgtag aaaggaagtg agttgatggc tgatctgcaa
     8461 gagagaaata ggcttgtttc tgctgaagaa aggcgtctaa ataaattgtt taaggatatt
     8521 gataaggata aaaagaaggt cgtttctggt ttgattacgc aagcggcacg cttaaaaatt
     8581 cttcttgatg aaatgtggat tgatatttcc gaaaaaggtg attatgagct tttctctcag
     8641 tcagaaaatc aaaaacctta cgagcgggaa cgaccagttg ctaaacagta taattcgaga
     8701 gaccaagcat atcaacgtgt aattaagcaa cttactgact atttgccaga agaaaagcgc
     8761 gaacctacaa aaaatgcagc gctagatggt agtgatttgc tgtgacaaca ttaaagccat
     8821 tctattttga tgaatacgta gatttatacg aaaaaggggt aatccctttt aataaagaaa
     8881 gaattcagtt aattacttat ttaaaaaacg aagtgctagt tcgggatgat atctattttg
     8941 atgaagaaat gattcaaaag tttatcaagt acactgaaaa aaattttttt cctttagcta
     9001 aatatcaaaa atttatcaca cctttcattt tctgctatca aaaagaggac gacgaggtgt
     9061 tttttgatga aattctaaat agtattgcgc gaggcggcgg gaaaaatggg ttcatgtcag
     9121 caagagactc attttttatt agccctctct acggtgtcag aaattatgat gtaacgatta
     9181 cagcgaattc tgagaagcag ggaaaggtat cttttaaaga agtttatgaa atggtacaaa
     9241 tgcgcaaact ggaaaaacaa ttttatttga ctaagatggc tatagtcaac aatgtaacta
     9301 attcgatttt tagttatagg acaaacaatc ctaagactat ggatagtgca agagatggct
     9361 gcttagaatt tgatgaaatc catcagtttg aatcttcaga cctagttgat attcagcgta
     9421 gtgggctagg gaaaataaaa catcctcgaa ctttttataa tggtacaaat ggtcatgtta
     9481 gagaaggttt ttatgacaaa atgttggaac gagcacaaaa gatttttagt ggagagaaca
     9541 agaatgatcg cttgtttccc tttatttgta aactggataa cattgatgaa atggataatc
     9601 ctaaaatgtg gtcaaaggct aatccaatgt tcgaagaaga ttcagcatat tcaaaacgtc
     9661 tgttttctac agtgatgaaa gaatataaaa aactagaaga tgagccatca ggtaggcgtg
     9721 aatttgttgt taaacgaatg aattttacag aaggtgatgc agaatcagat attacgactc
     9781 atgaaaaatt aatggctact aaacaagaat tgccaaattt gaatggtcgt tcttgtgtgg
     9841 ctggatttga ttatgcaagt attcgtgatt ttgcaacagt gggtttgctt tttaaaattg
     9901 atgataaatt tatctggcag caacatagtt ttgctaggaa aaagtttcta gatacattca
     9961 aactgaaagc gcctattaag gattgggaga aaaaaggttt agtgacaatc gttgatgagc
    10021 catcaattga tcctcgtcat ttaattgatt ggttaaatga aaaaagaaaa atccatcaaa
    10081 tagaaatcgt ttgtgccgac ggatttcgta tggatttgct gaaacctctt ctagagaaag
    10141 aatcatataa ttttgagttt atcagaaata taagaggtgt gcagtcaaaa gttgctccta
    10201 ttattgaaga tggttttgca aatgaacggt ttatttttgg tgatgatcct atgatgcgtt
    10261 ggtatacaaa taattcgttt atcaaagaag acaaattagg gaataaaaca tttttgaaaa
    10321 aagagccagt aaggcgtaaa acagatgggt ttcatgcttt tttggcagct ttatataaac
    10381 gagaagaaat aactgatatg gattatgagg aagcatttga tatgttggat gagattgaat
    10441 tttaaggtat actccttatt gaaaggggta gttatagaag tgaaaaagag atattttcta
    10501 tttgttacaa tatttataat aatagcaatt tgtgttttga cagtattttt accacaaatt
    10561 actgagtatt taaattatcg gatatttacc acaaaagtag atgatagcgt tactagttta
    10621 agtaggctag aatttataaa aatatgtgca tctgtgtttg gtcctatttt gactgtaatg
    10681 gtatttgtga atactttaaa tatgcagaaa aaaacagagc aaaagcaaaa tgagcttgaa
    10741 gtaaagcaag ctaaggctga gaacgagaga gcaaaaaaag agctgttaac acagatagat
    10801 cgtgagttct attttttatt aaatatgttt attgaaatac aaaaagatca aaatgccaaa
    10861 caagctgtta ttgctttaca gaagttggca atttttaacg aaaagggttt taatcaagac
    10921 tatgcattta cttctaaaga tttatcagat agtgaaattg ggagtacaat tgaacaatca
    10981 ggaccgctga agggcacagt tgaatatata aaatttgaaa catttgatgt aagcaagtcg
    11041 atggggaatt atcaaacaaa aacaaaagac gttgctacaa gtttacacga tcagaaagta
    11101 gctatattga atgaacaata tgaaaagatg caaatatatc ttggacggta cttcaaaatg
    11161 tttcaccgaa tagttaaaac attaaatgag tattatgacg attataatga ttttgatgtg
    11221 aagaggtata caaaatatat agggacatta agaacacaaa tttctccagc tgaattccaa
    11281 gtgattttat tcaattctct atatataaaa cgaggttttg gcttaggaat tcaacttatt
    11341 ggaagtggtt tttttggaga tgattttgat tttgaaacta atcaacattt tgaaacaagt
    11401 atcaatgaac agtggtttct atcactatca actgttgaca gtgataactc tataaagcga
    11461 caaaaattat ctgaatatat taaagaatta ggatccgtag aatataaaaa aatagctaat
    11521 tttgaaagtt tatataaact ctttaatgaa actaagctct agaaataggg cttttttatt
    11581 tttctttgaa aggtggtgaa aacatgtgag cttatttgat atctttaagc agtcaataag
    11641 aaatgaagag ccttctgagt ggataccgga ttttgtatat ggagaggaag tatctgctcg
    11701 ggcatacctc aaaattatgg caaaaaatac tgttttagat tttgttgcaa gaactatgtc
    11761 aacgttagaa gttaagttta aaaataaaga tgggacatca gactgggatt atattctaaa
    11821 tgttcgacct aattctgata tgtcagcagc tacattttgg caaaaatttt tttatcggct
    11881 cttagatgac aacgaagtgt tagtaatatt tacagatgat aatcaattgc tgattgctga
    11941 tgattttagt cgaacggagt atgccgtgta tgatgatgtt tttacaaatg tgatggtaaa
    12001 aaattttgtc tttgaacgtt catggaacat gtctgacgtc atttatatgg aatataataa
    12061 cgaagaatta gaaaggttta cacgaggttt atttgaagat tacgccgcgt tgttcggacg
    12121 gattctagaa gttgcaatgc gtaacaacca aattcgtggg tctgtatcca ttgattcaac
    12181 gggaacggcg aatgatgaaa aaggaaaaga tggtaagact agatctcaaa gattacaaga
    12241 atatattgat aggatttgga actcttttaa aactaactca gttgcaattg ttcccaaagt
    12301 gaaaggcttt gagtatgagg aatacaccaa taaacaaggc gtatcgaatc aatcgctaga
    12361 tgaactgaat aagatgaagt cgtctttaat tgatgatgtt gccaacgcca taggagtacc
    12421 tacggcgctt atttatggtg aaaaagctga actggattca aatattaaag cttttcgtaa
    12481 attgtgtatt gctccactag ttaaaaaact tcaagacgaa ttggtggcaa aaataattag
    12541 tcgtcaagag tacaaaaacg gggaacgtat agaagttaaa aaagttctcc aaaaagatct
    12601 attagagtat gctactcaaa ttgataaaat aacatctggt ggtgcgtttt atattgacga
    12661 agttcgtgat gaagttgatt atgatgagtt gccaaataat gaaggaaaaa ttcgtattcg
    12721 tacgaaaaac tatgaagaaa ctgtgaaggg aggtgagaac gacaatgccg aaagctaaaa
    12781 aagtaccatt tcagtttact aacgaaattc agaatggtaa gcacattctc accttgagtg
    12841 gaaatgtaca gaaaaaatat tggcgtgatg atgatgtcat aaacgctaaa gatattcgtg
    12901 actctctaga tactgtaacc gatgatattg taatcaagtt gaacagccca ggcggtgatg
    12961 tgtttgaagg gattgagatt tacaattatt taaaagatca cccatcaaat attactgtag
    13021 aagttacagg gcttgccgca agcgcagcaa cgtttattgt cgctggtgcc aatgatgtga
    13081 ttatgaatgt tgggacttct ttgatgattc atgaagcttc tacatttgct tgggggaata
    13141 aacaggacat ccaaaaaacg ttaaacgcat tggaaacgat cgatgattct atcttagcta
    13201 tttattcaga taagactggc caatctgttg ataagttgcg tgaatggatg aatgaagaaa
    13261 aatggtttac agcagatgaa gctgtcaaat atggatttgc caattctgtg aagcgtgaag
    13321 aaccacaaaa tgaacctcaa aatattgcag cgatgattca agatgctgtt gccgttgcaa
    13381 tagcgaattt aaaggaacct gttactaata aaatcgaaca agaatcaaaa caaaaatcat
    13441 taatcgcacg attgcgaaaa ggagaataaa gatatgttaa aaattacaga taaaactgca
    13501 gatgcaaaaa aggcatttaa tgctatttct tcgaaagaag atgcaacacc cgaacaaatt
    13561 aatgctgctc tagaagaata tgtaaccgcc attgcagaag atgccggcaa acaggtgcgt
    13621 gctgaatatg aagaattgaa aaatgtgacg gacaaccgtg tattagaggc tcgtggaatt
    13681 cctacactaa cagcagaaga aacaaagttt tataatgaag ccgtaaaatc tggaggtttc
    13741 gattctgacg tagtttggcc agaaacgatt ttggaacgtg tttttgaaaa tcttcaaaac
    13801 gatcatccta ttttaggcct cattaacttt acaccaactg taggtcgtgt caaagtaatt
    13861 agagcacgtc gtaaaggtgt tgctgttttt ggaccattgc ataaagacct tgaaggccaa
    13921 ctagatgctg aatttggtgc aactgaattt gttcaacttg cgttgaccgc atttttctta
    13981 atttctaatg ataccttaga tttaggacca cgatggattg accgttttat caatctttct
    14041 ttaagtgaag cagtacgtga tgtttgggct aaaaaaatta ttacaggttc tggtaaagat
    14101 gagccaattg gattgcttaa agatctagat ggtgcggtca caagtggtgt atatccagat
    14161 aaagctagcg cagggacatt aacttttgcg aaagagaaga ttgttcttga acttgcaggg
    14221 gtgatgaaaa aattatctaa atacacatac aaagtagatg gtgacgatga aggtgaagtg
    14281 aaataccgtt ctgttgctgg acgagtgtat ctaattgtta atcctgttga ttactgggat
    14341 atcgtagcta gagttacatt tgcaaattta aacaatgtgt atgggactac gatgccattt
    14401 atcgctgttg atcatatcat cgaatctgta gacgtaccag aaagtaaatg tattgcatac
    14461 gttgataaag agtatgaagc tactcaatca agagctgaaa aagtctatgt atataaagaa
    14521 acttttgcaa tgaaacgcgc aactttgtat gctgtcgaca tgcttggtaa tggctatcca
    14581 acaaataatg atgcagcaca agtatatgac ttggacttta cagcggggga gtaatgtccc
    14641 ctgcaattgg tgagataaca ccgacgactg atggggcagt cattaatctt tcgtaaatgg
    14701 ggttagagta tgaacactga agaatttata aaagaatata aggcccgatt tcgtattttc
    14761 catacttccg aagatgagga tatcgggaaa cagttagaga gcgggtttgc cgatataaaa
    14821 tctctaatcg gcagttttga tccttccact tatgagaaag gaaaagaatt agtttatgaa
    14881 cgtactcggt atctacggaa cgaatcacta gagtactttt acgacaattt tcaaacaatg
    14941 attatggatg catcaattga tctggcaggt gctggatatg ccaattaaat caaattataa
    15001 gaagcctaaa attgtagctg gtgatctaaa tacgccagtt actttttttg agtttcaacc
    15061 taataagggt ccagaacctg gtgatgaaga aaaaaaggaa ctgtattttt gtacagcact
    15121 agtttacaac ccatcaatga aagatcgtga cattcttggc acaaaagaag gcgttacgat
    15181 aaaaatacgg gatccccatc aatcttatat tcctactaac aaacataaag taaggatatt
    15241 agattatcga tatggagata aagtttggga aataatcgat gtctcaccgg atttggaaga
    15301 aaattcaatt gtaaagatag tattagaatt aattacttaa atttggtgat aaatgatgag
    15361 taacgtaaag tttgaaggcg ttcaagaaac aatccaagct ttagaaaaaa agtatggaga
    15421 aaagaaaact aagacattaa caaaaaaagc aatcaatgtt ggcgctgaaa aagtagaaaa
    15481 acagttgcaa acggacatga ccgtttttaa agacaaaggt tatacgatcg ctgaggttgt
    15541 tcgcaagaac gctacttata gagattacaa ggcagaagct gaaatcggtt ggaatggtcc
    15601 acatcaacga tatagaatca ttcatttgaa tgaatgggga tatacacgaa agggcaaaca
    15661 aattagaccg agaggatttg gtgtaataac aaaatctctt aagaattccg agctgttgta
    15721 ctttgatgct gtaggagagg aggttaaaaa gagtttatga aagatatgct taatatcatt
    15781 tacgaggctc tttgtttgaa tgagtatatt cattcaatga cctacaacga gaataccgag
    15841 caatatcgta ttaaatttta tgagcaacct gaaaccgccg ataaaacagg tccaatgatc
    15901 acaattcgtc ctgtagatac acctaacgaa gcgtaccacg gaagtaacaa agagctttct
    15961 gtggagcata tgatacagat tgacgttgaa tcgaaatata gagcaacatg caagcagatt
    16021 caatatgaga taaaaaaaga gatgaaaaaa ctaggctttg gccaaatttc cggtcaaggg
    16081 ttagatgaat actttactga aacaaaccgc tttgtagacg cgcgtcgtta cgaaggaaat
    16141 acaaaaattt acgataccga atattagaaa ttaagacacg aaaataacgt gtcttttttt
    16201 attacccaaa attaggagga aaattttatg actttagtag gattcaaaaa aatgacaatc
    16261 ggggtctttg acagcacagg gaagattcca gcagcaaatt tatatgtaat tgaaggtgaa
    16321 caaaacaaag gggcgactgt atctgctgaa atcagtgggc tatcaaaaga accatcaaaa
    16381 gtttacggat cgaacatcgc ttactatgtc tctcaaaaag gaacaggcga tgtttcagct
    16441 acttttggat tgttagattt accaacagaa gtaaatgaca aaattcttgg ttacaaaacc
    16501 gatacaaaca aaatcagctt cttgggagaa gatactgagc caccatattg tgcaattttg
    16561 atggaatcgg aggatttgaa cggcgataca gcaatgcttg cgatgttcaa aggaaaattc
    16621 agtcgtgaat ctattaatct taacactacg acaaatgaag ctttcgaacc agaagcagaa
    16681 gaatatgtat tttcagcgat tgccaatgat gttgaaggtg atgctaaagg ccaaacagtc
    16741 gcaaaatatg ttggagacga agaagcttct attactggtt tgaaagagat ggtatttcct
    16801 gcagcggggg agtaactagc cccgttgttg gagcaatcac tcccactatt aatggggcaa
    16861 cagttgaatt aagttaggag gataactatg ccagatacat ttaaaatcta taaaaaaaat
    16921 ggtactgaat ttacaaaagt cgcagagggt gaaagtccac tttctattac tggaattgca
    16981 gcaaatactc aagttgctaa aggagattat caagctacgc gtgtggtcgg aggtcaagaa
    17041 tcagtaaaag tagatattcc tgcattcacc actcttccaa tttcagttac tggtgtgacg
    17101 ttggataaaa cgacagctga agttgaacaa ggcggaacct taaaattaac tcctactgta
    17161 acaccagcaa atgcaacaga caaaacagga agctggagca gttcgaatac agctgtagct
    17221 acggttactg gaggcaacgt tacagtaaaa acagacgctg aagttggcgg aactacagaa
    17281 gtttcattta ctactacgga tggcggtaaa gtagcaaaat gtactattac agttattgaa
    17341 aaagcagcgg gctagtcttc ggactagctt ttttatttgg aaaataatgg aggaatctaa
    17401 atggcaaaag taagaattga attgaaagaa gaatcaggaa agaaagtcta tgaaaacata
    17461 gacacaacag ggaaagatta tcgaaaagct ttagaaacaa tcaaaaagct aaatgaagac
    17521 ggagtaatga tttggaatcg tttagacatt tatttggatt ttgctgtagg tatcttcagg
    17581 gatagcaaat tgacagctga acaaattctt gatggcttac catctgagaa ggtaatgagc
    17641 acattggacg acatcctagg agaagtaatg gggattgaaa gtaatccaga cccagacgca
    17701 aaaaagtaac acccgaagaa gcagaagaaa tgtacttaaa cctatgcaga gagttagtga
    17761 aagcgggatg gtcactatct gacatagaag ataattcctt tgaaatgatg atgaaaattg
    17821 cgtgcacttc gcctaagaaa gagaaacaaa aagaagttga tcttaaagac ttcttgaaat
    17881 ccatgtagag aggaggtaaa catatggcaa atggaaaacc tttaggcaat atgattatca
    17941 aactagactt agacagttca gctttttcaa aaggtctagc cggagctaaa aacgctgtta
    18001 accatcaaat gaaagcaatg aaatcacaga tgcaagtaat gaactcttca ggaaattctc
    18061 tcggtgcttt acaagcaaaa tacaatggtt taggcggagt tctacaagca aatgaaaagc
    18121 aagtagaatt gctcactaag gcttataagg atagttttga ctctaacgga aacgctactg
    18181 cttcgactgc taaatacgct aatcaattga atcaagcaac agctagatca gcaagttacg
    18241 aatctcagat gaaaacaact gttggacaga ttgctcggat gaaagtggaa acggaagggt
    18301 ttacggggaa actaaaggct aattctgatg tattgattaa ttctgggaaa aaaattgaag
    18361 cctttggtaa aggagtttct gggataggat ctgccctaac tgttggagta acagctccaa
    18421 tagtggcagg cgcaactgct gttactagtg cagctatttc atgggaaagt gcttttgcag
    18481 gtgtaaaaaa gacaaatgat gaagttgtag actcgaatgg aaacgtagtt tattcctata
    18541 gcgacttaga gaaaggtctg cgtaatcttg ccacccaatt acctggtagc catcaagaaa
    18601 tagcaaatgt agccgaagca gccgggcaat taggtataaa gactgaaaac gtagttagct
    18661 tcactaaaac gatgatagac atgggtgaat cgactaatat gtcggctgaa actgcggcaa
    18721 cttctttagc acgatttgct aatattaccc aaatgtctca aaaagacttt gataaattag
    18781 gatcagtcat tgttgatcta ggtaacaatt ttgctacaac cgaatcagaa atcactgaaa
    18841 tgggattgcg gttagcggga gctggtaaac agattggaat gagccaaggc gaaattctag
    18901 gatttgctac tgctctatct tctgttggtg tcgaagctga agcgggtggg tctgcctttt
    18961 cgaaggtcat gatccagatg caactagctg tagaaaaagg atttggcgct tttgatcagc
    19021 tgaaacagat ggcagaagaa caaggtgttc cgtgggttaa tttagtaaat gctgtgagag
    19081 acggtggaaa atcgttaaaa gcagtatcag agcaaatggg attcacttca tctgatttaa
    19141 agaaaatgta taaagaagcg gacaattcga aaagttcgct tgaaaatttt gcgaatgtgg
    19201 caggtgttac aagtgaacaa ttccagaagt tgttcaagag tgatccttca gaagcaatca
    19261 tcaagttcat tcaagggctg aaagattcag aaaaacatgg tacttcagca atcaaagttc
    19321 tggatgacat ggatataaaa gaagttcgac tacgcgacag tttattgaga gctgcaaatg
    19381 ctagtgacat attcaaagga gcaattgaaa aaggaaacgg cgcgtggaaa caaaatacag
    19441 ctttaacaaa tgaagctaat aaaagatacg aaacaactga atcaaagcta aagatattga
    19501 aaaacgaagt gattgataca gcaatcgatc ttggaggtcc atttgtagac gcgttgcgtg
    19561 atggtctaaa tgtatcgaaa ccattgataa aaaacttagg gaatatggcg aaagctttct
    19621 cagctatgag tgatgatcaa caaagaaaca ttatcaaatg ggttggatta gcagcagcag
    19681 ctggtccagc gctgaagctt ttaggaactg gtacttcagt aataggtaaa gttacttcaa
    19741 ctactggaag attgacgaaa agccttgttg atttagcagc gaaagcagca gaaaagaagg
    19801 ctatggatgc ctttgcatcg tcggtaacaa cagctggtgc tgcagctgca aagacagcag
    19861 gagctggagg tattggcggg ttagtcccta tgatgggaaa aacagctagt gctgcaagta
    19921 ctgctgcggg cgccagcgga ataggcgcaa tgacaacgtc actaggtttg ttaggtcctg
    19981 ctctacttgg tattgttggc gttggtggcg cactggcagt tggctatgga gcatggaaat
    20041 tatttggtga agaagcttgg aattcctcgc aaagagtaaa gcaatgggga acagatgtcg
    20101 gtgaggagat agatagtact cttgacggag tacagaagaa tattgaaggt gcaaatggtc
    20161 aattcggtct tctcaaagag ggttttactg ttgatgatgc aaataatatg gcggctaatt
    20221 tcgcaaccgc tggaacatct ttggaaacat ctttaacggg tagaataaag gcattagacg
    20281 atgcactaaa agggctacct caatcagttc aggatgccat gaaagaagta gctgaatcag
    20341 aagaagaaac cttagggcaa tctctttcta aaataaaaga caacaatgaa gaaataaaaa
    20401 aaatcagaga aagagccgca aatcagaaca gagacttgac tgtttcagaa ctacaacaga
    20461 tacaggatct aatgaaagga acttctcaag cgtatgtgga tacgctcgat gtttctgcta
    20521 aagacagaaa aaagatacta tcagccatga atggtgatgt tgcacaagct acagaagaag
    20581 aagcgaagac gtggttacaa tctttagcga aacaaaaagc tgcaactacc gaaaattata
    20641 gacaaatgct tgaagaaaaa aaggcggctt taaaggaaaa gggagtaagc aaagaattca
    20701 ttgatgcatt agaagatgaa atgaatgatt acatcaaaac tacaaatcaa ggattcgata
    20761 gccaaatagc tgctataaca aataaatatc cacaattgat taacgaagtg agtttatcaa
    20821 atggtcaatt atttagcaaa acggcagctg ctatggacaa tactggtcag tatacaaaag
    20881 tttttctcga aagtaatgaa aagattttag cccaagcgaa gaaaacatcg gatgaaatag
    20941 cagataatgc cgaaaaaaat gcagaacaac ttggtatggt agctgatgag gctacgcatg
    21001 ctggatcaac ttggaatggc ttagttttag acgaaaaaac aggaaaagta aaaactaatg
    21061 tacaagaagt agtgaatgaa gcaacgaagg atttcactaa atggaatgaa ttgaaacctg
    21121 ttgttcatga tgcgaaacta acaacgaatg caaaagatgt catttccata gcggcagtac
    21181 aaaatggcta ttgggatgat atggaatgga aagaaaaaga agcattatta gaagatgagt
    21241 gttctaaaaa tgtatacaag gctttagaaa atgctggtaa atggaatgag ttagatattc
    21301 cttctaaaga agcaatccta acttcaaata ctcctgaagt tatgggtgaa actctcatga
    21361 atttgggatt atgggatact tacaaacttc aagtgaaaga tttagatttg aatgcgtata
    21421 aagtatacga tgctatcgat ggatcagaag aaaaattaaa tagttgggct caattagata
    21481 accccacaaa agaattgtta ttagataact cagatttttc tacaaaactt tttaattccg
    21541 aagaaatgtt taaacgttgg aatcagttat catctaatgc aaagtttctt tcggctaata
    21601 atactaaatt tacagaggtt cttttatcat ctgacagaat gtggaatcaa tgggtaaacc
    21661 ttcctgataa tgagaaaaga ttacttgcag ataacgaaga tttagctacc actgttttat
    21721 cttcagaaga aatatacgga agatggactc aattaccaga taatcaaaaa tatatgttag
    21781 ctgacaatac tgacttagca aacaaaatat tcgcttcaga agaatcatgg aatgcatgga
    21841 aagcaattcc agacacagtt aaaaacttgc ttggtaataa tattgattta ctggctaaag
    21901 tgaaagacgg gactgtaagt gtagaagaat ataacaaagt tatgccactt cttaaaactc
    21961 tatatggaga taacgttaat ttaaaagagg caatcagtgg ggctaaaagc tctatagaga
    22021 attacaacaa aaatattttt cctagtgata aaactgcagt aggacacgat gaggcttcac
    22081 aagctgctaa agatgcattt gaagcgttca acatcttcca aacaaaaata ccagataaaa
    22141 ttactaaaac tgtatcagct gactttatag gtcctatgcc gaaaaatgcg aaaggaacaa
    22201 acttccatcc tggcggagca gctatggtaa acgatcaaaa agggcctact tatgaagagc
    22261 ttataacctt gccaagcgga gaaactttta tacctaaagg acgtaatgtc gttttagact
    22321 taccaagagg ttcgaaagta ctgaacgcca ctaaaactaa acgtctagtg cctaaatatg
    22381 ctgatggtat aggaaacata acgacggttt catctacgct aaacttagat gctttaattt
    22441 tagctattaa cgagctaacg acggtattga gaactcaact gatggcagga acgactgatg
    22501 ctaaagatac taaactatca gatgaaacaa aagttgccga tccgattatt ccggacagtc
    22561 tttctgaaaa atcagatcag tatctaggaa ttggagctca atggttaact aatctaatga
    22621 atggttggaa ctcagcggtt cctcaatata tgaatagtga aatggttttt attacaaact
    22681 atctcaatgc gctaaaactt caaaacaatc ctaattatac gcaaggagca acatggaata
    22741 agaacttact gaatggatgg aatagtttaa ctgggacctt tattgctaca atcaattcat
    22801 tttgtaatca agcaatggtc acgcttagaa actacaacac tcctatgtac aacaatggcc
    22861 gaacgtggca gcaaaataat cttaatggtt ggaattcatt gtatggatca ttcatagccc
    22921 gtgtaaatca gcttggtaac gattcgatta acaaccttcg ttctaaaagt ggtggttttt
    22981 acaatgcagg gacattcttg ttgcaatctt taataaacgg aatgaattcg ttaggtaatt
    23041 ctctttctac tacaatgaac aatgtcgcta atacaatggt tggtggtata ggcaaaggag
    23101 ttaatggcgt catatcggga gttaactatg tgcttaagga agtcgaatca agtaagaata
    23161 tcggaaactg gccaattcca caatacgcta agggaactga tggacatcct ggtggacttg
    23221 ctatgattaa tgatcaaaaa ggcctagttc acgaagaata tgttcagatg ccagatggac
    23281 gtagttttat tgccaaaggt agagatcttc ttgttaattt gcctaaaggt gctcaggtct
    23341 taaatgcttc tttaacaaag aaattaaagg aacgtttaaa tattccacac tacgaaaaag
    23401 gtattggcaa tttagatatt gttgatttgt tagatgatga aaaaagaatg ttagaattct
    23461 taactagcaa agttgatttt tcaggcatta atgaaccatg gcttgatatg actaaatcag
    23521 gaacgtcatt aatgtccaaa gctgcaaata caatgcttca atcaaaatta agtgaattct
    23581 tcactcatgg caattttgat ggtgcagtaa atgccaatgg tgtttatcaa tatttagttg
    23641 atgtggcaca gaaagtaatg ggtaagttcc ccggacttac agtaacttct ggatatcgag
    23701 caggagatgc ctactatcac ggaaaacggc aagctatcga cttggcttat ccaggtattt
    23761 ctggagatcc gagatataca gcagcagcaa attacgcttt tgaaaaattc ccttcaaaaa
    23821 ttgcgtatgt cattacgaat ggacgtgtac gtgaccgcat gggattatct ggaacaaggt
    23881 caagcggaca atggacgaat tggccagatg gcgatcactt cgatcatatt catttaaacg
    23941 gttcgatggg gtctggcgat atattcaatg gaggagttgg aggaagtggt gttgcacgct
    24001 ggcgttctta tgtgtctaaa gcattgaaaa tgaatggatt accagctaca gcagcgtata
    24061 taaatgcctg gatgtcccaa atccaaacgg aatctggtgg taatgagaaa gcaatcggcg
    24121 gcaatgatgg gctcgccgaa ggcaatgcaa caggattact tcaaacgaaa cccggaactt
    24181 ttgcagctaa cgcttttcct ggtcacggaa atattatgaa cggatttgat aacatgttag
    24241 cagcaatcaa ttatgctaag aaacgttacg gagttgcagg tatgttgcaa gttatcggga
    24301 aaggacatgg atatgccaat ggcggcttga ttaccaaaga cggactatat cgagcaggag
    24361 aaggaaataa accagaaatg gttattccat tgacaagaaa aacaagagct atcgaactta
    24421 tgggtcaagc attagccttc ctttctggaa gcaataaaaa agaaacgaaa agtgagatta
    24481 attctgtaga taatagacat atcctacaag cgatagaaaa acagtctcgt atgacaaatg
    24541 atctgctatc attattactc tcgtttttta aaagtaacaa tcgatcagat aaagaactag
    24601 ctttagatat tcagaaaatt ttaatcagga ggacatagta tttgaggaca gtaatattaa
    24661 gaaataaatt gaatgaagaa attgatttgt ccacagaaga ttattttgct actgaaatgt
    24721 cgaatctagg ttttgaagtt acaaaagagc atattggaca atgggggagt ttcagagaaa
    24781 taggagagag tattgaattg tctgaatttc aatcatcagt tatgatttct gttcacggat
    24841 ttagagaaca acaattatat ggttcattag ttgaatttct ggctaatgga ccttttgaac
    24901 tagaattttc ttttgattca gaaacaatga tcaggaaatg tagtttgaaa tcattatcta
    24961 aaacagaaat tgattcccaa agctcattgc taacagatac tttgaatcta tattttactt
    25021 caagctggta ttctattaag cgtgaaaaat taatacagaa ttcaacttct ctcaatacac
    25081 gtggaaaagt ttactcattt gttcgaccat atgtctacac caaaaatgta tgggaaaaga
    25141 aaggtgtatt taagttcagt aataattctg tatatttgac aaatagtaca gagcgaatgt
    25201 ctccgttaaa aataaggatt attggagagt gctcaaatcc atattgggaa gttatccaaa
    25261 actctaaagt agtagcttca gatggctatt ttattaatat gaaagacaca caaatattag
    25321 aagtgtctag tttgctagaa gaccaaacgg caattttaaa agatgtaggt ggagttgaat
    25381 cttctgttta tcaacaacaa gattttacta aatcaaactt cgtccaagcg cctctaggag
    25441 aattcagcgt tgtttttcat gttggacatg caaatgtaga gattgaactt tatgaggagc
    25501 gtgatctatt ttgattttag ccattaccct ctttcaccga gatatgaatt tatataacga
    25561 acgtttaatt tcgactgagt tcgaatttgg aacggatgaa attggagaag aagtaagtaa
    25621 ttttattaca gataaatatg tccatgtaaa atcaggagat tttctactag caaaatttat
    25681 tcctagcggg aagattgctt attttggagt gattagctcg caagaagaca aaaaaataaa
    25741 ttgcaaagga ttactcagtt tggccgatag cgagataccg actgtaaaaa tatcaggtgc
    25801 taattatgaa gagcatattc gacgactgat tgaatactac ttattgaacg atccaacaaa
    25861 acaactaaaa aatattttgg aggtaaaaac agaaagcgtt acttctcatt cttatcaagc
    25921 tgttgacaca aataagagac gattgaatgc atatataata aatgggttca aaaaatataa
    25981 tgttaaatgg tatgtcaaag agattaaagg taaaaaaata tatactggta ttcgagcagt
    26041 tgatgattca atttatatta aagataattc atctgagttt aatgactggg atgtttttgt
    26101 gcaatctcct ggaccaggga acgagaacaa attattgatc gtagataaag caatgaaaga
    26161 tatagaaaat cccttggtat tgtctacgtg gtatttagat gacgaaaata atcttacgca
    26221 agattcaacg aataagaaca ttgcattgcc taccgttaat ttagtaaata tatatgatca
    26281 aactcaagaa gatagacctt cttatgaaga ggtagctaaa tcggagctta aaggcaacgc
    26341 ctattcacat gaaattaaag ttaatgttgt gctaaatgct agaaatatca atgtagaaaa
    26401 tatcgaaaca ggaatgttag cgactattgt ttacaaagag cagacttaca aatctgttct
    26461 aactgcctgg cgaatatcca gcagtaaaaa gaatatagaa ttgacatttg gtaatgtccg
    26521 aagtcgtttt atggattatt ttgaagataa tgggggataa aaaatggtta gcaatgtgga
    26581 tggatatcaa tttgaaaacg tgaaagtaag cgcagaaaat gatgctagac tttatcacgt
    26641 tttatataat cggaaaaatc aggttattga tggttacgat cagtctatga atttatcttc
    26701 aagcggatta acagtaaaag ttgctgcagg agcagcgatt attcaaggtc gtatggtcgt
    26761 tgttcgacaa gaagaaagta taacagttcc agcaaactca agtggttata tagcattaac
    26821 agtggatttg acacaagaag ttatacctgg atctattctt ccagaatcgg aagaatatga
    26881 atggactaat aatcaagtca agctagaatt tataacaaaa gttataaaag gtaatttaaa
    26941 caatggtgat aaggtctata atttaccact atgctcagtt aattctactg gatcaactgt
    27001 ttcaatctca aagatatcgg atagttacga gctgactctc tctaaaggag aaattttgtg
    27061 gagggggact gcgttaatgc atgatactca aactgtccaa ccttcaaaaa aaatttggca
    27121 gacagtttca ggttttttgt tagtatggct cccatacgaa aacgggcaag caattgagga
    27181 tagatatgta actacgcctt tttataagga gcgtataact tttactaatg ttttaggaga
    27241 aattgtttca ggatttgatg actatcacaa aaagtggttt agtaaacgaa taaactataa
    27301 ttcgaatact aatatattta caggtgcagc aagcaatgca agtggagata acgcaaatat
    27361 ggtgcttagg tatatagttt ctttttagtt aggaggtatg gaagagtggc aaatttagaa
    27421 attaaattat ctgcaaataa gagacagcct tatctacgtc accgtgttgt tggtagagtt
    27481 ggtgatgggg ggctcacaac aatcaatgta caacttcttg aggaagatga aattacacct
    27541 tttgtaatta atctaaacgg tactttgaaa tttgtgggcg aagtttcaaa cggtaactat
    27601 accgagggag aaccagaaat aatcgattcg actaatgggt taattagtta cacgttcact
    27661 aagtcaaatt tcagcacgag tcatcaattc aaacaagcat attttgaata tgtagatcct
    27721 aacggcaaaa aagtaacttt tcagaacttc atcatagacg tgttagaacg agtagatatt
    27781 aattcggagc aagcgaaata ctatatttct tcattggaaa aattacagag tgaaatgcaa
    27841 accactttca atcagttcat tagtgataaa caggtccaat acgatcaaat ctactcgaaa
    27901 tataacgaat tagtaagatt gataaatgaa tcagataagc aagtaaatga tcgtattgat
    27961 aaggcaaata atcgtattga tcaaaccaat cagcaaatcg gcgatctcgg caagctgaaa
    28021 aagatgtaca gtaacagcat tga
//
LOCUS       DACECJ010000029        28029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.029, whole genome shotgun sequence.
ACCESSION   DACECJ010000029 DACECJ010000000
VERSION     DACECJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.029"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(35..616)
                     /locus_tag="IWO09_001333"
     CDS             complement(35..616)
                     /locus_tag="IWO09_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0263934.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(799..1425)
                     /locus_tag="IWO09_001334"
     CDS             complement(799..1425)
                     /locus_tag="IWO09_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0263935.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1447..2778)
                     /locus_tag="IWO09_001335"
     CDS             complement(1447..2778)
                     /locus_tag="IWO09_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0263936.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2933..3334)
                     /locus_tag="IWO09_001336"
     CDS             complement(2933..3334)
                     /locus_tag="IWO09_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0263937.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3420..3626)
                     /locus_tag="IWO09_001337"
     CDS             complement(3420..3626)
                     /locus_tag="IWO09_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263938.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3721..5223)
                     /locus_tag="IWO09_001338"
     CDS             complement(3721..5223)
                     /locus_tag="IWO09_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0263939.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5241..5696)
                     /gene="argR"
                     /locus_tag="IWO09_001339"
     CDS             complement(5241..5696)
                     /gene="argR"
                     /locus_tag="IWO09_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0263940.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5794..6411)
                     /locus_tag="IWO09_001340"
     CDS             complement(5794..6411)
                     /locus_tag="IWO09_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263941.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6632..8005
                     /locus_tag="IWO09_001341"
     CDS             6632..8005
                     /locus_tag="IWO09_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0263942.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8080..9192
                     /locus_tag="IWO09_001342"
     CDS             8080..9192
                     /locus_tag="IWO09_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0263943.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9264..10901)
                     /locus_tag="IWO09_001343"
     CDS             complement(9264..10901)
                     /locus_tag="IWO09_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0263944.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11069..11677)
                     /locus_tag="IWO09_001344"
     CDS             complement(11069..11677)
                     /locus_tag="IWO09_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0263945.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12061..13212)
                     /gene="queG"
                     /locus_tag="IWO09_001345"
     CDS             complement(12061..13212)
                     /gene="queG"
                     /locus_tag="IWO09_001345"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0263946.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13231..13983)
                     /locus_tag="IWO09_001346"
     CDS             complement(13231..13983)
                     /locus_tag="IWO09_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263947.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14124..15074)
                     /locus_tag="IWO09_001347"
     CDS             complement(14124..15074)
                     /locus_tag="IWO09_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0263948.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15075..15914)
                     /locus_tag="IWO09_001348"
     CDS             complement(15075..15914)
                     /locus_tag="IWO09_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0263949.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15911..16657)
                     /locus_tag="IWO09_001349"
     CDS             complement(15911..16657)
                     /locus_tag="IWO09_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263950.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16895..18265
                     /locus_tag="IWO09_001350"
     CDS             16895..18265
                     /locus_tag="IWO09_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0263951.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18320..19216)
                     /locus_tag="IWO09_001351"
     CDS             complement(18320..19216)
                     /locus_tag="IWO09_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0263952.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19497..20174)
                     /locus_tag="IWO09_001352"
     CDS             complement(19497..20174)
                     /locus_tag="IWO09_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0263953.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20265..21170)
                     /locus_tag="IWO09_001353"
     CDS             complement(20265..21170)
                     /locus_tag="IWO09_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0263954.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21345..22799
                     /locus_tag="IWO09_001354"
     CDS             21345..22799
                     /locus_tag="IWO09_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0263955.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            22815..23846
                     /locus_tag="IWO09_001355"
     CDS             22815..23846
                     /locus_tag="IWO09_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0263956.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23853..25040
                     /locus_tag="IWO09_001356"
     CDS             23853..25040
                     /locus_tag="IWO09_001356"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0263957.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25196..26578
                     /locus_tag="IWO09_001357"
     CDS             25196..26578
                     /locus_tag="IWO09_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0263958.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26550..26936
                     /locus_tag="IWO09_001358"
     CDS             26550..26936
                     /locus_tag="IWO09_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263959.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27136..27804)
                     /locus_tag="IWO09_001359"
     CDS             complement(27136..27804)
                     /locus_tag="IWO09_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0263960.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7796 a         5733 c         5159 g         9341 t
ORIGIN      
        1 tgaagctgac cacttctgtc acaacctcta attattaaag aaccttttgc gaagttaaca
       61 atctttcttt gatcaattga aaattctgcc taagtagtga tagatcctgt gtgactttca
      121 acaataagat acttccctgg atttcagcaa tcaacgcttt ggcttgacgg tctgcttctt
      181 catcggacag tttttcttca gaaagtgcca ggatattttg tttcgtatac ttgacccatt
      241 gattgactaa ttctagtagc aacagacggt attcttcatc cactgtactc aactcaataa
      301 tcaaattacc tacaggacag ccatgataag tgatctgtgt ctcatgatca gcgaaaatcc
      361 agtccaacat ttcttctaat gcttcttctg gctgttttcc agcatttaag atgtcttcga
      421 acaattctgt ctgccatacg tccatatgcg catggatcac cgctagacaa acttcttttt
      481 ttgaagaaaa gtaataataa aattgacctt ttcctgtatc agaagctgct agaatatctc
      541 ggattgacgt ttcatgatag ccttttttat aaataagatc tttggtcgct tccaagatct
      601 tttcctttgt tttcaatgat attctccttt ttcatttctc atagtcctat attatacagt
      661 aaattcatca ttttttcttc ttaatcgttt aaaaaagact gtgccaaatt atctgacaca
      721 gccctcacta taaaactgtt ctcatatagc tagatgttca ttgattcaag aactgcgagg
      781 acaaaagcaa ttcctatctt atgatagttg ctctaatact tcctctgttg tcatcacttg
      841 atctgcaata aagccaaagt tcgtcaacgc tgcatccatt gcttcttggc caggagcacc
      901 aattgcatct gtcgctacca caacattgaa tccactctct accaattcac gcatatggga
      961 atcgctacac aagtttccgt tcattccacc aagaattact gtgtcgattc cattttttct
     1021 caattggagc gccaaatcat tgctttctgg tccaaagatt ttatgaggag agctgatgat
     1081 cgtattttca tccaaataag gtttcaattc ttcgataaaa tcggctcctg aaccttcttc
     1141 cactgattca tattgacttt ttctagcaaa catctgctgc tcaagcatca tatgttctcc
     1201 tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa taatgaggag aaataaagag
     1261 tttgtatcct ttttcttttg cttgttttac catttcaatc atattttcga tcgtattgta
     1321 tttttctaag atatcttttg tcaaaccaaa gccaccgcct gctggtttca agaactcgac
     1381 ttgcgggtca gtgagaacta ctgctgtatt tttattattg aacatggtgg attctccatt
     1441 cgttatttat tttaatacat catttagagc ttcactcatt gtcggatgag aatagatttg
     1501 atctctcaat aacgtataag gaagttttcc attcatagct agcgaaatca agttgatgac
     1561 ttcatgagaa tcttccgcat agatacttgc ccctagaatc aagtcagttt cagggtcgac
     1621 taaaactttg aatacacctt taggatcttc taatacttgt gctttaggga caccgcttgt
     1681 catatacttg aataatttgt aattgacgcc ttgtgctttg gcttgttttt ctgttaaacc
     1741 tacattggat aaagtcggag tgataaatac actgtaaggc acagctttcc ggttattcgt
     1801 tgttcgttta ttctcgccct tcaactgatc cataatgatt cgataatcat ctagagagat
     1861 ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc caaatgttgt ctgcatctgt
     1921 acgaaggtat tcatccacag cgattgctcc gcgatctgtt agtttaacat ctgtattttc
     1981 taatccaagt gatgctgtac ttggttttct tccagttgca gccaaaactt ttgacgcttg
     2041 gattgttact tcttggccat cttctgtaaa ggtgacggca gctgttgatt cttggtctgc
     2101 tacttgatct acagatacac ctagatggaa ttcgattccc gcatttgtca tgtcttctaa
     2161 aatcatttgc gcgatatctt catcttcacg tggaagaaat tcagaatggg catcaagaac
     2221 gacaacttta gatccgtatt cattgaacat tgatgcaaat tccaagccaa tatatccagc
     2281 cccgatgatc actaattttt ccggtaattg ttcctgatcc atcgcttgcg tagaatcaat
     2341 caagtagcga cttgtattca atcctttgat tggtaaaatg attggttcag aaccagtatt
     2401 gatgaaaatc cgttcgcctt caacaatcac tttttcacca ttattcgtca cttcgatttg
     2461 atggttagaa agaaaacgtg ctgtaccgtc catcactgtc ccagttgcct catcgctgat
     2521 catatgataa tttttggcgc gaagcatgcc cgtcaatttt tctttccgag caacagcctc
     2581 tgtaaagtcg attcctcttt ctccattgaa aatcaatgat ttagaaggaa tacagccgat
     2641 gttgatacat gttccgccat acatacgtgt tgacttttca acgactaaaa ccgattcacc
     2701 ttttgaagct aacgccttcg ctaaagtctt accgcccttt ccaaaaccga tcacaatatt
     2761 ttgatatttt tccatcattt ttccctcgct tcttttttac ctaccgattg gtacaaaaat
     2821 atgataaccg ataattgtct attctccaaa tcatctgctt tatttgtcaa aaaaaaccag
     2881 acaaaaagcg gtcctttcac gtttttagtc tggttcgatt tcctttattc ttctaaacaa
     2941 tcgttacttc tacatcgata tttcctctag ttgctttgga gtaagggcag aattcatgcg
     3001 catcttctgc aattttttgg gcttcttctt cgctgatgcc atcgatatgc acttctaata
     3061 ctacaccgat ctgatagccg ccatcatcta aattataaag agagacacgt gcttttactg
     3121 tggactctgc tgtcattcct gcttctttga tcatatgttg taccgcccca ttgaaacaag
     3181 aagcataacc tgctgcaaat aattgttctg gatttgttcc ttctgcatgg attcctggag
     3241 gagtgatttt catatgcatc gagccatttg gtgcctcgac ttctccattg cgcccgccat
     3301 ggttgatcat tgctgtttcg tatgcttttt tcattgtact gctcctttcg attgaccatt
     3361 tgctacttgg ctataattat agtgtatgac aagtaaggcg ctttctacaa aaattctgct
     3421 tattcgaata ccagaatgcc gtcttctact atttttccaa aaaacaaaaa aatactactc
     3481 gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa tcttttctac ataatctttt
     3541 atatgattga ttttaagata catatatacc agaaagttga ccccataaac caatactgtt
     3601 aaaaaaataa ccatattcgt taccattttt tcctcctttt gctggtctcc ctaccagcac
     3661 tcgatttaac cataccactt tttttgccgt ccaagcaact agcctttcaa aaagactggt
     3721 ttattctgct gttaatgatt caagatcaga aaaatgtccg aataattttg ctaattttac
     3781 taacgccaac ttgttctttc cgaattcatc attttctatc tgtaatacta gtaacggctc
     3841 atgtaagctt aaacggatca aaaaccagcc gtttccgtat ggctcttcta gatcatatcg
     3901 aataccttct tcattttccg ggtcgaagtt catccctttt tcttcttcca acagtcgctt
     3961 catttcttct aatatcttgt ttccttgttc gcgtggttca tcgctgaaca attttaatcg
     4021 gatttcttgt acttctacgg gctgtttcaa ctctttgatt tgttcatcta atgtgatacc
     4081 ttttcttttt aattctggca aaagcatcag gatctttgcg atgacatatg caccatcatc
     4141 cagaaaatag ttttccttga atgcagcgtg tccgcttgtt tcgatggcta gctgtgtatc
     4201 tttcccaaga cggttaagtt ctaatgcttt attgattaca tttcgatagc cggaaatata
     4261 acgaacttgg atgcctcctt tttcttcaat gaatctttgt agatgagaag aagtagggga
     4321 attcgtcacg attcttgttc ctggatgctc ttttaatacg attgtactta atactgcgat
     4381 caaattattt cgattgatca ctgttccaga agcactgaca actgcagaac gatccacatc
     4441 tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa acagcctgct tgatactttc
     4501 catcgcttct ttattatcag gatttgggat atgattagga aatcggccat ctggttccaa
     4561 aaattgggaa cctgttgaat ccgcacctaa gctattcaat actttttcag caaagaatcc
     4621 accagcacca tttccggcat caacaatgat tttccaacca tttagtggtt tgctttctag
     4681 tgttccaatc ccctttttaa tttttgtgac taaatcttgc gcataaggtg tcaaaaggtc
     4741 tgctttttct acgcttccag tttgttcgct ttttattttt tgctgctgtg ataggataaa
     4801 agcaatatct tctttttctg ctcctccttc tgaggtaaag aattttattc cattataata
     4861 atagggaaga tggctagcag tcagcataac cgcagcatcg cacgagaact gttcaaattg
     4921 agtggacata aacatagcag gcgtagtagc taattcgaaa tcgatgacat gtatcccttg
     4981 ttctttcaga ctttggatca acgcctcttt cagtgctggt ccgcttatcc tactgtcatg
     5041 accaatccca atcgtccatt ttttatttgg atcggttgat ttcttctttt tctttatcca
     5101 gtttgctagt ccccagccga tcttttttac catgtctgtc gtcaagttta cttcatatcc
     5161 ttcaactggt aatgcgattc ctcgaatatc cgatccattt tgcagcgctt tcatatcttt
     5221 catataaaag tcctcctcat ttattccctc attttttcca gcttttctgc aaatcgctgt
     5281 gcttcttctt ctgagcggca aataataatg aatgtatcta caccggccat cgttccgaca
     5341 acttcagggt aagccacttc atctagccaa ttcgttacca catcggcacc atttcgttca
     5401 gtcaatacga taaccatgaa ctgaacagac tggatacgca aaacctcgcg tctaattgct
     5461 gtacgcaaac gttcttcatt taaagaaaca gatggctggt taaataatgt atagcgtact
     5521 attttgtttt cattttgact ttttactaac ttcatttctc gaatgtctcg agaaatcgtt
     5581 gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa gcagctcttc ctgtgtccca
     5641 ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc gctgtttctt tttcatatta
     5701 gacacctctc aatgttgatt atagcaaaag ataagtaata tttcttcata ttttggtatt
     5761 ttttactttt attttttgtt ttcattcagg ttgctaacct ttttctattt taacacgcaa
     5821 aatcgtcttc attttttcag gtgctgattt tcttggatcg ctgatgtaaa tctctttgtg
     5881 ttctttagaa gctctttcat agccttcttt tgcgatgaat gcttctaatt tctcaaacgt
     5941 tgctggttct tcggcatagg gcccgacgtg taagatttgg gcacaaagac cttcttccat
     6001 ctttttgaat actagttgtt ctttcaagtc ttccggaagt ttgctagaac gctgaatggc
     6061 ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg atcatcaatt gataagaaaa
     6121 atgttctttt ttcatcactg gttcatgcaa atacttttct tgcagtcccc attgtccttc
     6181 cagtggatag acagtataca tctgataatc aggaatcagc caattttttt taggcgacat
     6241 gcgtattgca tagctgactg catatagaca ggctactctt cgtttaaagt cttcctgatt
     6301 cgggtcgccg atccctctga tgctaaagaa tggtttttta ggaacctcta gtatttgcgg
     6361 gctttttttc atactatagc tcttgtcttt acgccattca attttttcca tttttcttcc
     6421 tccgcgaaat tccatttagt tatttattat catacctctt tttgagtgac ttggctaatc
     6481 gtgttgacac tatttttcag tttgtgctag aataagtgaa gtaaataaat cgtacagata
     6541 acacgtttcg gcgtgtcgag agagaggcgg tttctatttt tataatagaa atcactctct
     6601 ttttttacga aattttttag aggtgatgta aatgacaaaa caaaaacagc tgcttttagt
     6661 acctgcagta attgcaacag ggatgatgtc ttttgcgggc gtattgatcg agacggccat
     6721 gaatgtaact ttcccaactc ttatcagaca gtttgattta acgactgcac aagtccaatg
     6781 ggtaacaacg atttatcttt tgatgatttc gatcattgtt ccgctgtcta cttatttgaa
     6841 tcgcaatttt agtctcaaaa aactcttttt agcttcaaat ctgcttttct tggcaggtat
     6901 aatgatcgat ttcttctctc ctaccttcat ggtcctattg tttggtcgat tgctccaagg
     6961 tgcagcgaca ggtatcgctc ttccgttgat gttccatatt atcttgacat ttgcccccct
     7021 caatcgacga ggcgcgatga tgggtgtcgg tactttgaca acggccattg ctccagcaat
     7081 tggccccact tatggaggca ttatgacttc acattttaca tggaatcata tcttcctgtt
     7141 tcttgtgccc gttttgattc tttctttgat tatcggctgg tttgctattc ccgagatggc
     7201 agtacaaaaa agtggttatt tggattggat aagcgtttta ggaatttgtc ttttgtttag
     7261 tggaagtcta acatttttga gtacgttggg aacagtttcc agctggattg ctttaatcat
     7321 cggcggtatt ggctggttcc ttttttacta tcgaagtaaa aaagtggagc atccactgat
     7381 ccgactgtct attctttcaa ataaaacatt ccgcttgttt ttgattggct tcttagttta
     7441 tcagttttta ttacttggtg tatcttttgt cttgccgaat ttccttcaga tcgttcaagg
     7501 aagtagtcct tttgtcgctg gattagcgat gcttcccggc gcagcggttg gtgctttgct
     7561 ttctcctttt tccgggaaaa tgctggatca atatggtccg aagaagccaa tttttgctgg
     7621 gctgattctt tctttgattg gttggctagc attattccta ctcataggac acgcttcagt
     7681 gggtgtattg attgcttgtc atgtgtttta tatgatcggt atcggattat cttacagcaa
     7741 tatgatgacc accggaatga atgcactgaa tgaagaacta caaggagacg ggaacgcaat
     7801 gttcaatacc ttgcagcagt tttctggcgc tgtggctact tcattagtag cggtcattat
     7861 caattacgtc cagcatcata cttcccataa ctatgaagtg tctactacat taggatcaaa
     7921 agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc tttgctcgat tcgcctatta
     7981 tctgttcttt gctaaaaaag cgtagattcc atatacggca ggtgttgcat ctgccgtttt
     8041 tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta tggaaaaaat tgccattatc
     8101 ggtggcggga tcattggtat gactctagcc agttacttag atacatcaaa attcaatgtt
     8161 gctttatttg atgaagggac aggacaggca actaaagcta gcgctgggat tatctcccct
     8221 tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg ctaaagacgg cgctgctttt
     8281 ttccctaaac ttataaaaga tttccaactt gatcaaacga tttacgcaca atcagggaca
     8341 cttttgcttc gacctgaaaa agatttagct gaattggctg ttcttgccga gaaacgaaaa
     8401 gaagaagcgc cagaaattgg aaacatcgaa tggctttcac ctgaacagac atctcaagca
     8461 cttccgttat tgaaaccagc agtttcactg cgtatcagtg gtggtggtcg tctggatgga
     8521 aaagcatatt tagaacatct tggtcagcgt ttagcacaaa aacaaatgga gcagatccag
     8581 gcaaaagtca ctttgcataa gaagagcgat tcttggctca tcaagggacc gtttggagaa
     8641 tacacagctg atcgtgtaat tttgacacct ggcccggcac ttaagccttt attacaagaa
     8701 ttaggctatg ctgcagatat acgtccgcaa aaagggcagc tcttagcatt tcagacatct
     8761 tataagatga gtcaagactg gcctgttgct atgcttgacg gagaagctga tttcattcct
     8821 ttcatggatg gaaagatcct tttaggtgct acccatgaaa acgatcaagg ctgggatttg
     8881 gctcctacta gcgaagctta taagggattg actaagagta cagaacagtt cttgactgat
     8941 ccagatcaga taaaaaactg gccatacgag ttccgtgtag gtacccgtgc ttatacgtca
     9001 gatttttccc catttttcgg cgtactgcca gatgatccaa gcttactggc tgctagcggt
     9061 ctaggctctt ctggcttgac cacaggtcct tatatcggct atctattagc caacttctta
     9121 aatactggtc attggtctac ggaagattat caaaaggaca tctccaccta tattacgaaa
     9181 aatccatcat agaaaaacag ctttttcaag taaggatccc gagcgatatt gcgcatggat
     9241 tacttcttga aaaagctgtt attttagttc aatttctgaa tcaaattctc caaactttct
     9301 tttgcttcat ctggcaagag atcttttcct atctgagctt caatgaatcg taaacgaata
     9361 tcaagtcgtt gtttcaccag atttttatag gacgtatcct caaaagaaac aggaaaatct
     9421 ggtataggta aatagtggat gtcttcaaat ggaccaaacg acttgaattc tgccgtttgt
     9481 tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt tgattttttt atcatcgaaa
     9541 tatccagtat tcaggacata gcaggctgtt tgctgtttca aaaacaactc tcgaaaagct
     9601 tgatagtctt ccctaagagg atagacacgg aaaggattag caaatggttc aatgactagc
     9661 tcatctagct gtctttttgt agtgatattt tccgcgtctg atcgttttgt tgctaatgtt
     9721 gcaccaaaag acgctgctaa aactgggtca ttgatcttga taattggcgg aagcgaatca
     9781 tctttcatga tccaaaaaac agcatcgatc ttttcgctaa gatgatcgac tcggtcgggt
     9841 gtcgcataac gtgatttgac tgtccgccca tttccgtttc gtatatctcc tgttactagg
     9901 actttttctc ctttacttgt acatgtcacg cccacattct gacaagtaag gaaagaagaa
     9961 atggccggat catcagacgg atagtcttgt gttttatcaa agtaagaagg ttccaaggca
    10021 gtagcaaaac cattttttcg agaaataacg aaagcatcgt catgcaagat cgaaacagag
    10081 tgaccttccg tatgttttgc taaggtaatc gttgattttc cagaaccgga caacccaaat
    10141 acagccatcg tataggtctg gtcattacta gcatattgtt tcatcccacc gtggcaggca
    10201 ataaagccat tgcgatttcc caatgcccaa gcaagcgtca aagtcgcttt ttttaattct
    10261 ccaaagtaac gtaatcctag cacagccgct gtattatgga gagcatcaaa caaaatgagt
    10321 ccatttggaa atgtttcatg tgtccattcg ggatcacaaa agatataaag atctccctca
    10381 tcaaaggaaa cagacttttg ataagtctct aagatcttcg aattcgctgg ctgaaaattc
    10441 agcaaataag aatacaaatt gttctcaaat ccttctggaa tcagcaaatg actttgcaca
    10501 gaaaattctt cagataggcc aacgattact tttgtatggt aaaactggcg cttgcgactt
    10561 tgatacacag cttctcttaa aattttctcg tatttgcttt gttccttgtc taagtcctca
    10621 aagatccgcc gagcacttgc tgttcttccc acaatgcttc catcattgaa tactagtgtc
    10681 ctaacatcag ctggtaaatc tagttcttca gcatgtgaga ttgttaaatc tgtgatgatc
    10741 gttcctggac tcccttttgc tagttgataa gctgtcttcc tgtcaaaaac ttcatgaaca
    10801 ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac tggataacag tggatttcca
    10861 cttgtgattt cgtttaattc gagactctct tttgtactca tggctcctcc tcattcccta
    10921 aacaaaaaaa cgttttcttt attatacatt attcgagttt ttatttgtac tgctgcgctg
    10981 tgggaagcaa acgagttatt tttcacacaa aaaaaccgag aacttgaata aacaagtttc
    11041 tcggtttcat cattttcctt ttatcggctt agtaccagcc gtttgctaac cagaatgttt
    11101 tagcagcatc ccatgaacca taacgagatg ttacatattg ttctgctaca cgttcttgat
    11161 tggcagctga atagtcgccg tttaagtatg aagcatctaa ttgataacgg ccgatgtaac
    11221 gtccgttagt agctgtgtat gaaccgcctg attctttttg tgcgatccat tcttttgcag
    11281 agcttgaagt tggcgctgtt gcagcagctg gggcagcagg agctacttca actgtttcaa
    11341 caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt tgtagatgct gtattttctg
    11401 ttgttttgtt ttctgttgtt gctgaagaat catttgaagt tgggattgtt aattgttcac
    11461 caacataaat gcggttgatg tttgcgatcg aatttttttc agcaattgca tcgatcattg
    11521 agttgtctcc tgcaaatttt tgtgagatct tagataatga atcacctgat tttactgtat
    11581 aaacttcatc agcgtgagca tttgtagttc ctacaaacat tgcagtagca ccagcagcaa
    11641 aagttgtacc taaaacaatt ttcttaaatg aattcatatt aaactccttt ttttctccgt
    11701 tttttattct ttcgacgcta ttaactttat catttagaaa tatttcatag gtaaaagaaa
    11761 catgacattt aatgacaaaa taacgaatta tttaatattt gtaacattta aacaaatatt
    11821 tttttgatac aaaaccataa aagccttgtt gtataagcaa agtaatcgtt tttctatgtt
    11881 tttttatttt tgaatgaaaa acgcagattc tgtaacattc actgcaataa tccataaaaa
    11941 aaagcaaaaa aatttttgat ttttatcaaa ttctcttaaa aaaatgtaaa aaatacaaaa
    12001 aaaccagtaa ttgttacaac aattactgga caaaaacttt gaataatgtt aactattttt
    12061 ttattttaac tttctccgca ataatttctg ggcatgttcc aattcgttta taacgacttc
    12121 ttctgtttct ttagctaata tttcatctaa tacatcaagc catttttccg gttcttttgt
    12181 tcctagacgt ccaatagccc aagctgctgt tcctctgatg acagggcgga catctgttaa
    12241 acattctgaa atctcaggca atgccgttcg atcacctaga ttagctaaag caatcaatgc
    12301 gtttctctgc aaaggtttct tcccacgcca tgaaccagct aaatgcccaa attgctgttt
    12361 gaagtcttta ttagacatcg aaagcattgg ctttaatttt ggatatactt cgtctacttt
    12421 tggttccatc tcttcatgaa aatgaaaatc ttttccttga ttgtaagggc agactaactg
    12481 gcagatatca catccataaa tgacatttcg cattttttta cgatattctt ccggcatcat
    12541 cccttttgtc tgagtttgat aagacaaaca tcgctgggca ttcattcttc cgtcaccaag
    12601 caatgctttt gtcggacaag cagtcacgca gcgcatacag tcaccgcaac caaattctcc
    12661 tggtttatca ggctcaaata caatattagt aacgatctca ccaagataga caaatgaacc
    12721 gaactcttca gtgatcaaca atccgtttcg tccaataaat cctaaaccag ctcgctgtgc
    12781 tacagcgaca tcgatcagtt ctccagtatc tacttgcgga gctagacgcc attcctcttc
    12841 tttttgccat ttttctgctc ttgtttggat gaattcgatc aatcggttca aacggtctct
    12901 gagtatatca tggtaatcga tcccccaaga agcacgagca aactgcccgc gtttttcatc
    12961 cctaggcact tcttcatgga tcctagtagg atacgcaagt gcaatggaaa tgatcgattt
    13021 cggctgatca aatgtcattt ctggataaag acgctcatcg atatttggat gttcaaagcc
    13081 agaagtatgg ccagctgctt tttgttcttc caacgattct tttaatgaat caaaaggttc
    13141 cgccgtggtg aacccgattt tgtcaattcc taatcgtttg ctttcttgta tgatttcttc
    13201 ttttaatgtc atggtcttcc tcgcttgctg ttaacggctg tataacccat aatatagttt
    13261 ttcttttttc agctggacaa actggatctg ctgatcggca atcttctttt cttccatcca
    13321 tttttttctt gcagcaaata cttcccaata atttgttgaa attaattttc ttttgttatc
    13381 taatgcatta aaaaaattca atgaaactgg ttttgtactt aacagttcat ggagccttac
    13441 ctcttcccat ggcgaaaact cagacagatc gcgggaatac aatacttctt catagagatt
    13501 tttttgctgt gtgtacgtga atgtttgcgg gattttccca ggcctagcaa tttctactag
    13561 acgattataa aacatattca ttaatttgct gttggcaata aattgacttt tataataggc
    13621 cgactggatc tttttgatta ctccttctgc tttgatgact gaaaataatg caggccatgc
    13681 tttgacggtt atttcgtcgt tttgtttaac taatacgcca ggtgtgaagc cgactggaga
    13741 aaggaacaca aaataaccga taccggtatt ttcatctaaa ggctgaaatt catcaaacaa
    13801 ccatttgcct atgtacagct cttcatctgg ttctgttgtc tcattttgat ttgctatgaa
    13861 taatttagct aattctgctt caagaaattg aaggtcttga tccttgtcgg tattttcctg
    13921 ctggctgatc aatcgaaaga cttcttcttt aatcgtatat ttatctttac gtgtaaaatt
    13981 catagccaca ccttccttcc ttactctaac tataacggaa tttcttcaaa aaaggtatct
    14041 gtacgacgaa aaacagcata attttttttc atatctctta taaaaaatgc aaggtctgct
    14101 cctcaagcta gaccttgcac ttttcagctt tcttttgttt gtgacaaacc ttcatggatc
    14161 gtttcaatat tccatttcat catctgatag taagaatctc cttgttctcc ttcttttgct
    14221 atggaatcag tgaacagttt atcatagatt ttcaaacccg tttcttttgc tacccgttcc
    14281 atgcttcttg agtctacact ggtttccacg aataaaaccg gtacttcttt tgctcttatc
    14341 tgatcaatga tcgctttcat ttgatcaggc gtaccttgac tttctgtatt gatctcccat
    14401 atataagctg ctggcaaatc atatgctcgt gaaaaatact tgaatgcccc ttcacttgtt
    14461 actaataatt ttttgttctc ttcgatagaa gcaaaacttt ccttcgcttg tgtatctaat
    14521 tcttttagtt tttccacata tgcttctgca ttttttttat agagtgtcgc attttctgca
    14581 tctttttcag agaatatacg tgcgatttcc tccacatatt ttattccgtt agataggtct
    14641 agccaagcat ggggatctgc ttttgtatgt tcttcaccgc tagttaaata aagaggttct
    14701 acatttttgc taactgcaaa ataatctttt ccttcttctt ttttagccgt ttccatcaag
    14761 ttatcgaacc agctgttacc tgtttcaaga ttcaaaccgt tgtataaaat aacatcagca
    14821 tcgcttgcct ttttgatgtc ttctggtagt acttcatatt catgcggatc tgttccgact
    14881 ggtacgatac tgtggatatc tatttgatct gttccgacct cttttgccat gtccgccaag
    14941 atcgaattgg tagccacgac tgacaacttt tctttacttc cttcatccgc ttggtcagaa
    15001 gcctgctttc cgcaaccggc taataccaag aaactaactg ccaagattaa aaagagtgat
    15061 ttttttgttt tcatctatct gcttcctttc tgaaataaaa ttccttttgt tggagaaaat
    15121 aaaaaagcaa ggataaaaaa tgcggtacat gctaacacga tcgtagctcc tgatggccaa
    15181 ttgtaactat aactgaaaaa cacaccgaca actgcactta gcataccgat tcctgcagct
    15241 gtaatgatca ttttcagtaa atggttcgtt agcaagtaag ccgtggcggc tggtgtgatc
    15301 aacatggcaa tcaccaagat tgttcctact gtctgtaaac tgactacagc cactaatgtc
    15361 aaaaagaaca tcaaaagata atggatcagc gatgtgttca gcccataagc ttttgccatt
    15421 gtcggatcga atgatgtgat cttcaattct ttataaaaga aaaagacaaa tatcaagacg
    15481 attccactaa cgagtgctgt cagcaacaaa tcgctttccc taacagctaa gacattccca
    15541 aataaaatat gatacaaatc tgtcgcactc tgggcatatg aaatcaagat aatccctaat
    15601 gcaaaaaagg agctaaagac aattccaatc gctgtatcat ttttcaatcg gctccgctga
    15661 gtcacgaaac caatcactgc tgctgttagc atcccaaaag cagtggctcc aaagatgtaa
    15721 ctaaaaccaa acatataaga aatggctaca cctggaagaa ctgcgtgaga gatagcgtct
    15781 cccatcaaag acatccctcg taaaataata aaagaaccga tgactccaga agcaagtcct
    15841 acaagcatgg aagtgaccaa agcgtattgc aggaattgat aattcattaa tccatcaata
    15901 aaggattgga tcattttttc tctcctcctt ttactaaatg tctgatgatc tcaccatagg
    15961 ccaattgaag gttcttcgat gtaaagactt cttgtactgg accggaagcg atcagctgtt
    16021 tatttaaaag aataacctca tcgaaatatt cttctacttt atgaagatca tgatggacga
    16081 tcaaaatcgt ttttcctgaa ttcttcaatt cctgcaagat gtcaaagatc tttcgttcac
    16141 ttaacgcatc aatccctacg aatggttcat ctaaaaagat ccattctgct ccttgggcta
    16201 gagctcttgc aataaaaact ctctgaagct gtccacccga tagttcgctg atctgtcttt
    16261 tggcatattc ttccatccct acttttttca aagcttgtct tgcacgttct ttttcttttt
    16321 tcccaggtct ttgtccaatt cgtaaagatg gatatgttcc taaaagtaca acgcctaata
    16381 catcaattgg aaaggaaaga tccaattcac ttcgctgttc cacgtaggct atttttttac
    16441 ggactgagtt taccggctgt ccagaaaagg tgactttacc agtagtagct ggtatcaact
    16501 ctagcaaaga tttcataaat gttgattttc ctgcaccgtt tggtccgata ataccggtaa
    16561 ttttttgtgg atttatcgtc aagttgatag ttttcaaaac agttttccct tgataagcgg
    16621 ctgaaaggtc ttgaattaca atggccgtac gttccaaatc ttacactcct ttttaggttt
    16681 acctaacttt ttatttaaat atagtttaac taaaatggaa agcattttca agtagaatga
    16741 tgaaaaagtt tttttcgaat tttcctcgca ttttatgaaa attttctgta gaatcacatc
    16801 aagagttcaa taaggctaac ctaataatga aaaacatgtg ttgaaacgtt tgattatttt
    16861 ttcttgttga ctcaacaacg aaaggagttt ttgtatggaa gaagaaaagt taactcgaag
    16921 tcttagtgcc cgtcatatcc aaatgatcgc actaggtgga acgatcggtg tcggtctgtt
    16981 tatgggtgcc tcctcaacca tacgttggac agggccctca gtgatgggag cctatgcgat
    17041 tgcaggattc tttttgtatt tgattatgcg tgctttaggc gaaatgcttt acgttgatcc
    17101 ttcgactggt tcttttgcaa attacgcgag cgagtacatc catcctgtgg ctggctactt
    17161 gaccgcttgg agtaatattt ttcaatatat tgtcgtagga ataagtgaag tgattgctgt
    17221 aggatcttat atgaattatt ggtggcctga tttgccggca attattcccg gaatcattgt
    17281 cgtcttgttt cttatgctgg caaatcttat ttctgtcaag gcgtttggcg aattagaatt
    17341 ttggttttcg atcatcaaag tcataacgat cattttgatg atcattgcag gtcttggggt
    17401 aatcttattt ggatttggta atcatggtca ggcagtaggg atttcaaatc tttggaaaaa
    17461 tggtggattc ttcactggag gcgtcaaagg atttttcttt gcattatcga tcgttgttgc
    17521 ttcctatcaa ggaatcgaac tgatcggaat gactgctggt gaagcagaga atcctaaaaa
    17581 gacgatcatt gaagctgtcc agtccactat cggtcgaatc ttgatttttt atattggtgc
    17641 tatttttatc attgtgagta tctatccgtg gaatcaattg agtgagattg gttcaccttt
    17701 tgtacaaact ttttctaaag tcggcattac atttgctgca ggactgatta attttgtcgt
    17761 gattacagcg gcattatcag gttgcaattc agggattttc agcgcaagtc gtatggtcta
    17821 tacacttgcc ataaatggaa agatgtctaa aaaatttctg aaactatctc gtcacggtgc
    17881 gcctttttat cctgtcgtag caatctcagg tggaatctta attggtctga tcttaaatta
    17941 tcttctacca atgttcatca accagtcaga aaatattttc gttttcgtat acagctcaag
    18001 tattttgcct ggtatggttc cttgggtagt gattttgatc agcgagattc gcttcaaaaa
    18061 gaagcacgca gataaaatgc atgatcatcc ttttaaaatg ccactatctc cttatacgaa
    18121 ttatgtgaca cttgcttttt tagctatcgt attgattttc atgtttatca atcctgaaac
    18181 gagaatctca ttgattattg gactcgtttt cttgctttac atgacgatcc attactttgt
    18241 acgcgaaaaa agaaccggaa aataaaaaaa gaacgaagct tcaccttagt ggaagcttcg
    18301 ttctttttta ttgcttttat caattcaatt ttagcgtctc accgggagtc aggaaataat
    18361 tctccatgct catcccattc agcgacaaga tcgtttctac tggtacgcca gttcgttcgg
    18421 ctatttgatt tggaccttct ccttccaaaa ctacggttgt cgcagcctgt gaagtcgcgc
    18481 tgttggtatt agtttcagag gaatctgttg tgtcttgtgt tgttcgagct tgcatttcag
    18541 aagcttctac cgactctgct gtctcagaat cagaagatgt tgtgtcttgt gtgcttgttg
    18601 actctgcttg aagagaggcg ctagtctgca aataaatatc ggaaatctta gcgacatgat
    18661 tcgtataatt agccgataat tgattgatca aagtatctaa gtctacaccg tctttatttt
    18721 ctgctgcctg atacgctgca cgtaattttc tgatcccttc gtcggagatt gcttgaagct
    18781 cacgtgcttt ttggttagca actgctgata aaccagctat tccctcttga tttccctgaa
    18841 tttctgtctg gtattcttca atcaatgcag gcgtcttttc ttcgatcttt ttcgaataat
    18901 catttacgat tgcttgaagt tgctctggca aaccatttgc tgttttttct tcagaagttt
    18961 gtgtctcttt tgttttagtt gatgactcac ttgtttgttt cgtctctgtt ttctttgtcg
    19021 ttgtttccgt tatggtcgta cttttcgctt ctgacgaatt ttttccatga aaatatagac
    19081 ccgcaactcc tcctgctatg atcaaaaaca cggcaactgc tgccccaatc atgatcatcc
    19141 gcttacgagc tgcctgtttt tgctgtcttc ttcgggtcat ccgaacattt tctccccact
    19201 cattttcatc atacattttt ttcaccacca atttttatgt ataatagcgt tatcaaacgc
    19261 ttcatattct tattgtagca aataattgtc gctgtgcaca tttttatctt tgttatttat
    19321 aagaaaattt caccagttga gatgattcca agattgtccg tcgatttctc cttgcagctg
    19381 cattgttcgt tcgtctatct cagccatcag tttctttatt tcatataaga gcgcacggtc
    19441 ttgatattct gatttttcgc ttaattcatc taattgttgg atcagccaat catttgtcat
    19501 aatgaagttc ctttcatttc ttgcagtgta aaggtcaagt cttcgatctg ctcttttgcc
    19561 tcagtcagtg cttctacgat atatgcttgt ttttcagatt cttcttcctg cattttctga
    19621 ttttcctgta ctctttgaat aaaccagtca atccgctgag tcagcgatgg tttgccttcc
    19681 aatgagagat actgctgata agcaattagc ttctcccaac gtttttcctg ggataaatac
    19741 ataaattgtg ctaaaacgaa tggcggaaga taggtcatat ggaatttatt tgcgattcgc
    19801 tgatacggcc tcaaaaattc ggaaatcgta tattcttctt ttcctccagc ttggtattct
    19861 ttttccgcca cacctgttgt caatacgatt cctagttctt tcccttttaa tacatgtgct
    19921 gctcgttcca agacattatc ttgccaaatt tttaaatgag caggagaact ataccagtaa
    19981 aacggaaact ggaaaatgat ccgatcatgc tgggccaaga gctgtagttc agtctctata
    20041 tctatttttc cgtctggata acttgattcc aaatgatgcc aagtagctgg taaaccagca
    20101 gaagcttctt tgaaaaattg ctgatttaaa gattcttcga tcgttggatg gctaatgatg
    20161 ataagtgtac gcatcccttt tcctcctttt tagtcttcgc tttacagtgt agcataaccg
    20221 ccacaagttt cttttgccga tgcttgtcat tttaaccaga ctaatcaggt tgtccaagaa
    20281 actgcggttt gtttttccaa ctgttttttc aaggtatcca aagcaatatt cttaaaattt
    20341 tcgtttttca tacgtttacg atagttcgta gccgtttctc cggtactctt tttgaacatt
    20401 cggctaaata cgactagact attgaaccct acttgatagg caatactttc cacggattca
    20461 tccgtcagag aaagaagaaa cgcccctcga ttgatccgta ccccgatcaa atattccttg
    20521 atactggtac ccatatgatt tttgaatact cgcgacaaat ggctccgatt gaccgctaaa
    20581 tgctgggcga tttcctcaac agacaatttt tcagcataat gtgcctcgat gtatttgact
    20641 gtttcaacag ctaaagagga atgaaccttt tgtacaccac cgctgacctt tccgccatct
    20701 tttaaaagga ctgataaaaa acggtaggtt aatgctgtta actgtaattc attttccaag
    20761 gtttcttccg tatctttcat gcattctaaa ataatgtcga tatatttttg tatgtcagaa
    20821 gagaccatcg tgtattggtc ttctctgaaa agcgactgcc gaatgatttc ttctgcagca
    20881 ctcccaccaa atgaaagcca gcaatacatc cacggatctt ctccatctga taagtaaaac
    20941 gtcgagtctc ctggacgaat caagaacaga tcaccttttt tcaattggaa ttgttgattt
    21001 ttcacatgat aagatccttt gccttccatc acgacatgca ggatgtaatc attgcgaata
    21061 gcaggaccaa aagaatgaaa cggcatcgtt ttagagaaac cacaaaaatc aaaatacaat
    21121 tctttttcta cttgttgatg ttcctttttt aaaaaaatcg attgctccac gtctatccct
    21181 ccttactcct ttagtataat gcaacaaaaa gttaaataaa agtcacagaa atatattaaa
    21241 aaggaagcga tttcttgtta agtttaagtc attaaaagat ctttgttatt cgttgtaacc
    21301 ggtttactta gtattatata ttgaacagga gaggaataca gattatgaaa agcaaaatga
    21361 tccaacgtgt gtcctacgct ttaggcgctt ttggacacga cacattctat gttacattaa
    21421 gcacttattt tatggtattc gtgacaagcc agatgttcgc aggaacagat aaggcaacga
    21481 atgcaaaaat gattgctaca gttacaagct tagttgtagg tatccgctta gttgaaatta
    21541 tttttgatcc tttgattggt ggcatcatcg ataatactcg tacaaaatac ggtaaattca
    21601 aaccatggtt agttatcggc ggcgttgtta gttccgtact cttggtagca atctttacca
    21661 atttctttgg cttagctact cataatacga ctttatttat catattattt gcaatcgttt
    21721 tcatcttatt ggatagtttt tattcattta aggatatcgc tttttggtca atgattccag
    21781 ctttgagtac ggaaacaaag gaacgtgaaa aatttgctac gtttgctcgt ttcggctctt
    21841 ctctaggtgc aaatggtact actctagtcg ttgtccctat cgttactttc tttacctatc
    21901 ttgttactca taaacatatt gaaggcgctt caggctggtt ctggttcggt gtgattgctg
    21961 ctgttatttc aggcggtact gcgattatca ctgccttggg cacaaaagaa gtcgatactg
    22021 cgatccgtca gcaaaatgaa aaagctggga tcaaagatgt gttcaaagca gttgctcaaa
    22081 atgatcagtt gatgtggctt gcccttgctt atatcgctta tgcgattgcc aatgtagcaa
    22141 caactgccgt attattctac ttcttcaaat atgtacttgg ccaaccaact gaattttgga
    22201 tcgttggtct agttgctgcc ttacttggtt tagtagctgt tccactattc ccaatgctta
    22261 cacgcgtcat ctctcgtcgc tacgtttacc ttggagggat cgcaatgatg atttcttctt
    22321 actttttctt tacgatcgca ggttcaagtt tgccaatcgt tatcatcggg ctagtattgt
    22381 tctacttccc tcaacaattg attttcttat ccgctttgat gacgatcaca gactctgttg
    22441 aatatggtca atggaaaaat ggtatccgta acgaagctgt tactctatca attcgccctt
    22501 tactagataa aatcgctggt gctttgtcta acgggatcgt agggtttgtc gctatcgctt
    22561 ctgggatgac tggtaatgct actgctgctg atatcactgc tcatggcgta acgactttca
    22621 aagcttatgc attctatgct cctgctgctt tgatgatttt ctccgctatt atttttgctt
    22681 ggaagattac attgacagag aaaaaacatg cacaaatcgt tgaggaattg gaaaacaaat
    22741 tggctccagc tgatacaatg gaagatgaac ttcacgatgc agtagttcct tcccattaag
    22801 aaaggatctt gaaaatgaag attacagaaa aagattttgg ccaaggttat cacttgataa
    22861 ccttggagaa caaaaacaag ctagcacttt ctatctctga tttaggtgct agaatcgtca
    22921 gtttaaaatc aaatgatcga gaattagttt tgggatttga caccgctgaa gaatatatcg
    22981 aaaaagatcc gtatatcggt gcttctatcg gtcgcaccgc agggagaatc gaaaatggcc
    23041 gcttttctct caatggaaaa acttaccaat tagccacaga tccaaaaact ggacataatc
    23101 tgcatggtgg tgctcctgga tttgaattaa aaaaatggtc ttatgtcatt ttgaatggcg
    23161 aaaatgaagc ttctgtcatt tttactacta caagtcccga cggagaacat ggttttccag
    23221 gtacaatgga tgtggaaatc cgctatactt tgacaaaaga taatatctgg cgagtgacct
    23281 ctcgcggcac cagtgaccaa gatactttat tcaatccgac aaatcatgtt tacttcaact
    23341 tgacgggaga tgcttctcag tcaatcgatc aacatgagct ttggctcaat agcgaagctt
    23401 atgctccttt gcgaacagat agtattccta taggggtaaa agaaaatgct gcaggaagtg
    23461 ctttcgattt tcaaatacct aaaaaattag cgtccgtttt tgctagtgat ttggatcaaa
    23521 aaaatcttgt tgacggaatc gatcatccat ttttcttaaa agaaaccggc ttgggtaaag
    23581 aagccgctcg cttgactagc cccgataaaa gaatccaagt aggtattgct acagatgctt
    23641 ctagtgtggt tattttcact gcaaatttcg gcacagaaac accagaaatg cgcaaccgaa
    23701 aactggcaca tcacggtggc atcacatttg agactcaaac agctccaggt gcagagcgct
    23761 tctcagcatt tggttcgatt catttaaaag ctggatcagt atttgaaaca gtgacagaat
    23821 tcaaaatcaa aacaagaaag gaataactca tcatggaaat caaaagaagc ttaaacacaa
    23881 aatttgccga aacattcgga cctaaaaaaa ctacacaata cttctcacca ggacgcatca
    23941 atttgattgg tgaacacaca gattacaatg gaggccatgt cttccccgct tccatcactt
    24001 atggaacgca aggtgtagca gcacctcgag acgataataa aatattagtt tattccacaa
    24061 actttgacga tatcggtgtc attagtttta ctttagatga gctagaatat gataaaaaag
    24121 caaactgggc aaattacgta aaaggaatgg tccttaaatt aaaagaagct ggacactcaa
    24181 tcgatcatgg gtttgagttg ttagtggaag gaacaatccc taatggtgca ggactttcca
    24241 gtagcgcttc attagaatta ttagtaggtg ttgtattaga agatctgttt gatttaaaaa
    24301 cagatcgctt ggaactagtc caaacaggta aaaaagtaga aaatgaattc atcggtgtca
    24361 attcaggtat catggatcag tttgccatcg gcttcggtga gctagatcat gcaatcttat
    24421 tagatacaaa tacgctgaaa tacgaaatgg ttccagtgaa actagatggt tatgcaatcg
    24481 tcatcatgaa tacgaataag cggcgggaat tggcagattc aaaatacaat gaacgccgta
    24541 gcgaatgcga agaagccttg gcacgtttgc aaaaaacttt gcctatctct tctttaggag
    24601 acttagatga agaagaattt gaatcaacta ttgaccaaat cggcgatgat acattgatac
    24661 gcagagctcg ccatgccgtt tctgaaaatc agcggacttt gaaggcaaaa gctgaattag
    24721 aagctgggaa cttagaagca tttggtcaac tgttaaatga ttctcaccac tctcttcgct
    24781 atgattatga agtaactggg atcgaattag atacactagt agatgctgct caaaaacaag
    24841 aaggtgtctt aggtgcacga atgacaggag ctggatttgg tggctgtgcg attgctcttg
    24901 tcaaagaaga aaatatacct gccttcaaaa acaatgtcta tgatgaatac atgaaagttg
    24961 tcggctatgc tccggaattt tatgttgcac acattggaaa tgggacaaca aagatcgatc
    25021 cagaagctgt tcatgtttga taaaaaaatc aacgatctca gctaaaaaca aatagataaa
    25081 aataacctct cagcaatttc caaatgtgag ccttttgctc gtatttgaaa attgctgttt
    25141 tttattgaca cggaaagaga acgtgtgttc taattgtgtt gaagaggtga ttgttatgtg
    25201 ggcgaatcaa aatccggtat ttgtttatga aaaagaacct tcaagagata ttttatgtat
    25261 tgactgcaaa tctttttacg cctccgtaga gtgcgttgaa aaggatcttg atccccttac
    25321 aactaaatta gtagtcatgt cctatccgtc cgattcgaca gaaacacggg gtagcggatt
    25381 aatcttggct tccagtcctg cagcaaaaaa agcttacggc atcaccaata tcagccgtgc
    25441 cagagatctt cccttcccct atccaagtga tttatatatt gttgccccaa ggatggccta
    25501 ttatatggaa aagaacagag aaatcaattc tatctataaa aaatatgcag acgaagcgaa
    25561 ccatcatgtc tattccgtcg atgaaagctt ccttgatgta acaaatagca tggcactctt
    25621 tggtaaaaca aatgcgtacg acatggctaa actgatccat aaagatgtgt ttgagcagac
    25681 cggaatctac acgactatag gaattggcga taacccgcta cttgctaaac tagccttaga
    25741 caatggctca aaagacggaa agcaaatgtt tgctgaatgg cgttatgaag atgtaccaga
    25801 aaagttatgg aaaatctcag atatcactga cttttgggga atcggcaaac gaacagcaaa
    25861 acgtctgaaa caaatgggta tacattctgt ttatgattta gctcacgcgg attattatca
    25921 cttgaaaagc aagctagggg tcatcggtac tcagctttat gcccatgctt ggggcatcga
    25981 tcgaagtttt ttgggacaag aatatacacc aaaatcaaaa agtatcggaa atagtcaaat
    26041 cctagataga gattatacaa gacgagaaga aatcgagatc gttatcaaag aaatggccga
    26101 ccaagtcggc actcgcttga gacgatctgc agcaaaaaca caagtagtca gcttatgggt
    26161 aggatactct ttaggctatc ttgatccaaa tggcaagacc gggtttcacc agcaaatgaa
    26221 aatcgaagcg acaaatgcta gcagagatct agcagatgct ttattgacca ttttccacca
    26281 gcattaccac taccaggata ttcggcgtgt aggcgtgaac tgttccgacc tcgtatacgc
    26341 gacaggttta cagcttaacc tttttgaaga tcctgaaaag cagataaatg aagcgaaagt
    26401 tgattttgtt gtcgatacga ttagaaaaaa atacggcttt caatcaatca tccatgcgca
    26461 tagtctcttg caaggaggac gcgccatcgc acgtagttct ctcgttggcg ggcatgcagg
    26521 tggtatgagt ggattagaag gtggaaaaga tgagacggac agcaaaaaaa ttcattgact
    26581 ataacgagta tcgagatcgg ccatttggat taaaatgggg gactgctttt gcgatggaag
    26641 aactagtcaa cggtatcaaa aaaaatgaag aagaagcacg caaacagcct cttcctaaac
    26701 aacaaatgac attgcaagaa atcgatagta tactgacaga agccttttta tctcgtaaag
    26761 cagtaatcat tcagctcaat ttaaaagatg aatttggaag actcttggat gaggtcgaag
    26821 ggcacttcat tggtgaagcc tatgaagatt actttatgat tgaccatcag ccgatattgt
    26881 gggaagacgt acggtatatt gagttaaagg aaacaaaaaa atggtttgtg acatgagagg
    26941 ttgtgacaga agtgatcaac ttcaaaaaat aagaaaggat ttttcgaaaa ttgcttctta
    27001 aatttttgtg gaattttggc ttatttttcg aagaagcaac ttctggaaca acgtatatta
    27061 gatatcagag gttgtgaaaa agtcgcttag ttttgagtaa caagccaagg cgctgatttc
    27121 acaacctctg tctatctatc catctattcg gtaagattca tgcatgacgt cttctttatt
    27181 tagtatgtag agcccttttt cttctgacca ttcaacataa atcagctgtt ttacatctgc
    27241 atagccttct ttcttcaagt tctccataag ccatgcttca gtcaattgat tggactgaag
    27301 cactttatgc tgaatttgcc cttcatctac aagcaggatc gataaagctt tgtcttcatc
    27361 tgattttttc aatacactca tttgaccagc attctctagg ataacatgtt tggcattttc
    27421 aagagaaaag cattcttgct gtcttaagat tgctcgcaac tgctccatct cgatatggtt
    27481 atttgtcaac tctgtcatgt tgatgacgcc gtctttgatt agaacagatg gctcgccttt
    27541 gacccaacga ttgacctttt catttttttg gacgatacgt tcgataccat aaatcattac
    27601 tgcccaaagc gcaatggcaa ataggacatg ccctacatgg acattgtcat cataggttga
    27661 ttcttctaag atcccaccta aaacaagcgt atagactaaa tcaaacggtg tgatttctga
    27721 catggatttc ttcccaagca aacgtaccac taataataaa ccgatcaacc cgaacaccag
    27781 tttcattgta attgaaaaaa tcattttttc tccccctaac tatctgttaa ttaaattata
    27841 tatgctttct cacgaacacg catggaatat acaaggaaga aatgtatcct tttactaaaa
    27901 aaaggactgt gacactgtag tgacccccaa aagttagact tttttggagt ggagaaatcc
    27961 actccttttt tttatgctct aaaatgatat ttttacacaa ccgttacggt tgttttacga
    28021 aattggacg
//
LOCUS       DACECJ010000030        27237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.030, whole genome shotgun sequence.
ACCESSION   DACECJ010000030 DACECJ010000000
VERSION     DACECJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.030"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            149..1012
                     /locus_tag="IWO09_001360"
     CDS             149..1012
                     /locus_tag="IWO09_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0263961.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1141..2763)
                     /locus_tag="IWO09_001361"
     CDS             complement(1141..2763)
                     /locus_tag="IWO09_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0263962.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3012..3533
                     /locus_tag="IWO09_001362"
     CDS             3012..3533
                     /locus_tag="IWO09_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263963.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3719..5449)
                     /gene="spxB"
                     /locus_tag="IWO09_001363"
     CDS             complement(3719..5449)
                     /gene="spxB"
                     /locus_tag="IWO09_001363"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0263964.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5614..6432
                     /locus_tag="IWO09_001364"
     CDS             5614..6432
                     /locus_tag="IWO09_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0263965.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6535..6732)
                     /locus_tag="IWO09_001365"
     CDS             complement(6535..6732)
                     /locus_tag="IWO09_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263966.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6901..7479
                     /locus_tag="IWO09_001366"
     CDS             6901..7479
                     /locus_tag="IWO09_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0263967.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8524..9027)
                     /locus_tag="IWO09_001367"
     CDS             complement(8524..9027)
                     /locus_tag="IWO09_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0263968.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9386..10411
                     /locus_tag="IWO09_001368"
     CDS             9386..10411
                     /locus_tag="IWO09_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0263969.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10734..11612
                     /locus_tag="IWO09_001369"
     CDS             10734..11612
                     /locus_tag="IWO09_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263970.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12088..12204
                     /locus_tag="IWO09_001370"
     CDS             12088..12204
                     /locus_tag="IWO09_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0263971.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13132..13470
                     /locus_tag="IWO09_001371"
     CDS             13132..13470
                     /locus_tag="IWO09_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263972.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13564..13794
                     /locus_tag="IWO09_001372"
     CDS             13564..13794
                     /locus_tag="IWO09_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263973.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14194..14565
                     /locus_tag="IWO09_001373"
     CDS             14194..14565
                     /locus_tag="IWO09_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0263974.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14839..15531)
                     /locus_tag="IWO09_001374"
     CDS             complement(14839..15531)
                     /locus_tag="IWO09_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0263975.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15534..16319)
                     /locus_tag="IWO09_001375"
     CDS             complement(15534..16319)
                     /locus_tag="IWO09_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0263976.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16556..17956
                     /gene="lacG"
                     /locus_tag="IWO09_001376"
     CDS             16556..17956
                     /gene="lacG"
                     /locus_tag="IWO09_001376"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0263977.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17981..18298
                     /locus_tag="IWO09_001377"
     CDS             17981..18298
                     /locus_tag="IWO09_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0263978.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18311..19984
                     /locus_tag="IWO09_001378"
     CDS             18311..19984
                     /locus_tag="IWO09_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0263979.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19998..21629
                     /locus_tag="IWO09_001379"
     CDS             19998..21629
                     /locus_tag="IWO09_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0263980.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22501..23691
                     /locus_tag="IWO09_001380"
     CDS             22501..23691
                     /locus_tag="IWO09_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ0263981.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23825..26014
                     /locus_tag="IWO09_001381"
     CDS             23825..26014
                     /locus_tag="IWO09_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0263982.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26011..>27237
                     /locus_tag="IWO09_001382"
     CDS             26011..>27237
                     /locus_tag="IWO09_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0263983.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         8737 a         4488 c         5386 g         8626 t
ORIGIN      
        1 ttcttatttc ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg
       61 aaaataaacc ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt
      121 aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga
      181 tctgccatac catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt
      241 gaatgatcaa gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa
      301 tcagctgaaa aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga
      361 atttttccgt tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg
      421 attaagcggc tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga
      481 agaccctgag atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt
      541 attggaagct gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct
      601 ggaaagtaat tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga
      661 acgaggcggt aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc
      721 tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa
      781 caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca
      841 aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt
      901 ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag
      961 tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta
     1021 actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat
     1081 agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca
     1141 ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata
     1201 tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa
     1261 tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa
     1321 gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac
     1381 aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc
     1441 agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa
     1501 gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa
     1561 aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt
     1621 tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa
     1681 tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa
     1741 attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta cattttctac
     1801 ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct
     1861 agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc
     1921 ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat
     1981 ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc
     2041 caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat
     2101 atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc
     2161 aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg
     2221 ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac
     2281 cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag
     2341 accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc
     2401 tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat
     2461 cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag
     2521 tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg
     2581 tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt
     2641 tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat
     2701 attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat
     2761 caattttttt cctcactttt atcattatta gttttgacaa gtcttataga attatatagg
     2821 ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga
     2881 aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca
     2941 gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag
     3001 aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt
     3061 tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg
     3121 tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca
     3181 gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata
     3241 ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg
     3301 tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa
     3361 gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat
     3421 taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg
     3481 gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat
     3541 caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct
     3601 tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa
     3661 aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc
     3721 acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt
     3781 tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg
     3841 gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct gattttgcag
     3901 ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga
     3961 gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt
     4021 ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa
     4081 taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc
     4141 catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc
     4201 ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt
     4261 tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg
     4321 catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt
     4381 cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg
     4441 ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg
     4501 tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat
     4561 cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt
     4621 cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag
     4681 cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta
     4741 gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc
     4801 cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca
     4861 aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg
     4921 tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag
     4981 ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt
     5041 gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta
     5101 gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta
     5161 gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc
     5221 ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga
     5281 gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata
     5341 gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc
     5401 agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact
     5461 cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa
     5521 atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt
     5581 agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa
     5641 ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc
     5701 gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt
     5761 gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa
     5821 gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt
     5881 gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa
     5941 gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg
     6001 cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat
     6061 aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac
     6121 ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag
     6181 gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg
     6241 ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca
     6301 atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa
     6361 cgtcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg
     6421 gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc
     6481 gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca tacattaata
     6541 atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat
     6601 tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact
     6661 gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt
     6721 tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag
     6781 ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc
     6841 aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc
     6901 atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt
     6961 ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt
     7021 taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat
     7081 catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc
     7141 tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat
     7201 agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat
     7261 atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg
     7321 ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta
     7381 gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg
     7441 atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt
     7501 aagagaaaaa tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca
     7561 aagagggatg cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac
     7621 gatgcttatt tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt
     7681 tttgacatta ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct
     7741 atttaagaaa agcatgttta cagcagtatg attctgttgt aaacgtgttt ttatttgtgg
     7801 aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc cacagtggat
     7861 aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag ggcgtataga tttcttttga
     7921 taggagatat acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt
     7981 gtggtttttg gaataaaaaa ataacatatc tcattttacg cttttgttat tttatactta
     8041 aaaaataacg acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact
     8101 gtttttttcc tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc
     8161 aaggagaagg gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata
     8221 aaacaagctt tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc
     8281 ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa
     8341 aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc
     8401 ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa
     8461 taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg
     8521 attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa
     8581 taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt
     8641 tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt
     8701 gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag
     8761 gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac
     8821 cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc
     8881 aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg
     8941 tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc
     9001 taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg
     9061 aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg
     9121 aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta agatagcgag
     9181 ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt cgttttttgt
     9241 tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc aagaaatagt
     9301 tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc agcttttttt
     9361 ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca ataaaataca
     9421 agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat tatggttagg
     9481 ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct ttactttttt
     9541 ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga tcggttgcgg
     9601 actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc ttgaaccatt
     9661 tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc atatttctaa
     9721 accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc agctgccaat
     9781 cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg ccctgattgc
     9841 ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa aagcaacttt
     9901 aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct acagcgctgc
     9961 cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt attggttgac
    10021 cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc atcgacaagt
    10081 ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc
    10141 gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg cacaatttct
    10201 agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt tgatgctatc
    10261 tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt tagggatcgt
    10321 cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt tgcaacatta
    10381 tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc aaaagaggtt
    10441 atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga ttctcagcat
    10501 taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta tatttattct
    10561 atcaaaaaat tttgtttatg atggtaactg aataaacttt cctgagatgg tcaatgcttt
    10621 ccttgaaagt cttacagata ttctttcttc atagaaagtt taagttttat agttagtgta
    10681 caataaaagt atgtttatac catatggtgt ccggataatc agtcatttaa acaatgaggt
    10741 tcaaagatat gaaaagggga aatttgatga agaaaataag caatgaaatc caaattctgg
    10801 cagaaacttt aactcttccc aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca
    10861 tccttgatga ggtgtttgag caatatagtc atgaactaat ggatcctaat aaagcaaagg
    10921 cgactgcaaa gaaaatagac aaagtctatg aagaggaacc ttttgcagca ttagctattt
    10981 atctatatgc agcttctcaa agttggaaaa aaatttatca accattgaag attccaagaa
    11041 ctatctattt ggcaacaatg aatgctttta ctagatttat tcatgaacat ttccaagtaa
    11101 caggaagcta tcgctttgac cgtggttttt ggatttggca ttacactagt ggcttgatct
    11161 ttcggattgg tgaattggag tacgaaatga catattttcc gaaagggtac acggatctaa
    11221 gattagaagg aaagcagtgc ctttctcttc atattccttc cgatgcagat ttatctcaga
    11281 ataagataag agatagttac gaaagtgcca ttcgtttttt tgaactttat tttcctgatt
    11341 accattacca agtgatgtac acagatactt ggttactatc acccaatctt actaaatggt
    11401 tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta tcgattatta tcagttgatg
    11461 aacaggacga tagtggagtc ccttggatct ttggacgagt agatgcccaa atacatgatt
    11521 atccagaaag tacgagttta cagcgacaag caaaagagca attgcttgca ggcgagcaca
    11581 ttggctctgg cttaggtatt cttacttcat aagtttagta cttcgaaata gtttaaaaga
    11641 gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
    11701 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
    11761 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt
    11821 ctatcgaaca cacagtacgt tcaataggta aactagctat aacaatagat attaaagtta
    11881 acggacaaga gggccttttt taggtagcta aaacgtaata atgaaaattt atttccagag
    11941 ataggagtca atcagctagt ctgatcgact gttttaacaa gttgactttc attttaactt
    12001 ttgataccat ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta gcatgactac
    12061 aatcaaagaa gaaagtgggt cgtccatatg gaaaaaacag atatctcaag tgcataccgc
    12121 cgcttgaaaa gcccaaatat caaaacacga aaacgtgcgc tgaagatcat caaagatgtg
    12181 aaacggaata gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa
    12241 cgtttttcgg ctattttcaa taatcggttt ttgtgtaagg tttccgaaag aactgctgtt
    12301 gattttccat ttttaacata tgataaattg gtagaatctt tcttttcaaa ttgataccat
    12361 tccctaattc cagttatata gttaactaca gaaccacaaa gcacgtacgt ttataaaagt
    12421 attatctgta gttatttttc aattcccttt caacctctta ttttgaccag tagtattgat
    12481 cactttacct tttaacacct ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg
    12541 taactacata atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc
    12601 aatacggttg caaataacca ttatcgttta ataagcagtt acgaaagctc ccaatatctc
    12661 taacgttcag ctcactccat ttcttttctt caaaaaaatc agtgattcgt atctctttct
    12721 atttgtcgaa aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa
    12781 agcagttact attgctagaa taacatcaag gaataataag ttttggtttt cagataatag
    12841 tcgataagtt gtgtattgga attttagata cagtaataaa gtatttttca tgaaattttg
    12901 tgagaatcac tattacaagt atctttaata aaattaatgg ttaaggagga acttaaagta
    12961 gaatgtaaat gaatagttgt agtcagtcga ttgtgatact agactcaaag ttgcaggaat
    13021 aagtttttca atttgtgatt ggaagataaa aaatgaaaaa gaagggcttt tttttatact
    13081 ttgttgattg tagtgtttat tttccctctg tttagccaag taacattttc tatgactcaa
    13141 ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta cagaagagtt taagaaatat
    13201 caggaaaata ttgttccgga gactccatta gctagtgagc caattactga tattgatgga
    13261 aaccaaatag gatggtatat acagtacgaa gttatggtgg taaataatga ctcaatagta
    13321 aaaaatacag taggcttaga gctaacaccg attgaaaagg tgagttcatt gttaacgtat
    13381 atttatactg atgaaagtga cgagactgag atcattttaa cggattactc aaaaattgct
    13441 actgataaaa agtatatatt gtggatgtag acactagtga aatactttat gatatagaaa
    13501 ttgatgaaaa tttactagat gtagcacaag aaatcgatca tcaaaagcag gaagctattc
    13561 gatatgctgg tagaaaaggg aaaagaaaat atttattacg tgaatgtagc aaaagttaga
    13621 gaagatgaaa atgagtggaa agaattcaag agtcgctact caattaactc gactccgaca
    13681 tttacagttt acagagaagg atcgatagaa aaaactgttt tttggactaa agaaagtgga
    13741 atgtctttag ctgaagtaga agagtttttg gattatgtta gtatgcaaca gtaagggagt
    13801 tcttattatg aaaaattttg acacatatta tgttattgtt ttttcgatat ctttgctgtt
    13861 aatattacta actttaatgc agtataaaga gtatccagag tattttattt taacttttgt
    13921 atatttagta agtagctaca cacttctata taaattcaga agttcttcta gagtttctaa
    13981 agtttgtttc ttaattagct tattgattct ataatatttt tgatttatat aggaaattaa
    14041 aaatattata gaatcaacac tcagagaacg ggttggtttt taatagattt aataagtact
    14101 gataatgaaa tttctcctgg ttgggaaaac gaagttggtt accctgtaga agaatttttt
    14161 caagattact ttgcggagta tgccgatagg ataatgagag attacgtaac agataaaact
    14221 caagaagtca tcaatcaatc ccagtcatta aatgaattat ctgataaggc gaaagaagaa
    14281 gcagttgcca ttatttcttc agggattagt gactatattg atatgagtgg tgaaatcaat
    14341 tatggcgata aagacaaaat tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg
    14401 aaggcagatc ttaagtttaa tgatacttac ctagtaggaa aattaattaa tatccttggg
    14461 acagagaaag atacggaaac gtttatttcc tctttgtcta tgttgtttac agataagcat
    14521 ttttcaactc agcttggtgg agaggaacta gtggaaatct tttaaccatt acgcccagca
    14581 ttaataaaag tatatcaaaa aagtactcct gatgattttt aagattggtt tgagaaataa
    14641 tgtgaaaaca ctatttttaa tgttaattat agatagagat gaacgcgtca tatggttaaa
    14701 agaatgaaaa agtcaaagat acactgctat atttcactaa ggatatagca gtgtatcttt
    14761 catgtcgttt cactggcagt tttttataaa aggaggtgtg catttcagtt gcttttcttt
    14821 ggtagttagt tgaatcgttt aagaattaac ggatgtggca gacaggtgct ctgtccaaaa
    14881 accagtaaaa tctttgatgg ttccatcttt atttagctgt gcctgttcaa atactgtttt
    14941 gtttttaccg aatggatcaa ttgcaataat tgttccgatc cccgcacgtt tggcagcaag
    15001 taatcctgag taagcatctt cgataacaag acaatcagcc ggagctaagc tcagtttgtc
    15061 tgccgcttta agaaaaatat ccggttgcgg tttcccaggg aaagagccgt cgtcatagac
    15121 aatcttgtcc cagtcaaacc agcgggctaa atcgaaaata tcaaaataaa aagcaacatt
    15181 ctctttgaca gtagccgtgg caatcgttaa tggaatctgt tggtcagtca gctggtcaag
    15241 agtcgcttca agcccttttg tcaactgaag ctcgttttca ttttgcaagc aaagcatacg
    15301 ataatagctt tctttttctt ctgacaatac ggcgatctca gaggcggtca aatgctctga
    15361 aatgaaatgt ttcaaaatct ccgtattcgt ccgaccatga atatttttta aaatatcctc
    15421 gtctgataaa ccatcttttg tatgatcatg tatcatatgc agccaagcag cttcatgcag
    15481 atgagagtcc aaaaacattg ttccgttgaa atcaaatata atccctttca tagttacgct
    15541 cctatagtgg tattttcggt tgaatcagca atttgcagat cgacatattg tgcatagtgc
    15601 tcttgaacct cccgcgagat ctggcgatcc gtaatgagca gatcgaaatc atgcaaatta
    15661 taaaacacat aaaaatcatc tcgattgaat tttttattat caacgagtag atatttctta
    15721 cgggaattat ttagagcgat ctgctgtgct tctccttctt cggtactgta agtagagatc
    15781 gcttcgttgt gaacgccatt tgaactgata aatgcttttg taaactttag tcttcttaaa
    15841 ttgtcgttgg tgatcggtcc gacaaatgcg ccggtatttt tccggaaatc accgccaatc
    15901 agtagaattt ctggtccatc tgcttgagca agaatatcaa aaactggata attatttgta
    15961 atgatccgaa ttttcttgtt cagtaaatgc gtcgccaata gttcgattgt tgtacctggt
    16021 cctaagaaaa tcgtttctcc ctcgtgaatc aaggaggctg ctgtggcagc aatttctttt
    16081 ttttcttgaa tctgcagagt ggctttttca gtgtgagaaa gttcttgatc taaagtgtag
    16141 gaaagacttt gagcccctcc atggacacgc agcagcttgc ccgatttttc taattcatct
    16201 aaatctctgc gaacggtcat atcagaaaca tctaattgat tgatgatttc attaacggta
    16261 aggattcctt ttttattgac catttctacg atagtaagca atctttcttt tttcaacatt
    16321 gtcttcacct ctaatttaag gataatctat catataaacg gacaacagtc aatctaattc
    16381 gaacaaaagt aaacatttag ggtggttttt atgtttgtta caatagattt tattgctgtt
    16441 ttctggtttt tatgattatt tgtgtttgtt tttgttgaca aatgttttgg atgcgtttac
    16501 aatgcaatca gttagaaaga gcgaaaactg attatagatt tgaggagaga tgtacatgct
    16561 taaattacca gaagatttta tttttggcgg agccacagca gcctatcagg ttgaaggagc
    16621 cactaaggaa ggtggcaaag gcgctgttgc ttgggatgac tttttagagg aacaagggcg
    16681 attcagtccc gatccggcaa gtgattttta tcatcaatat gcaaaggaca ttgaattgtg
    16741 tgaacgtttt ggtgtaaatg gtctaagatt atccattgct tggagccgca tttttcctga
    16801 tggagctggg aaaccaaatc cagaaggaat cgctttttat catcgtgtgt tcgaagaatg
    16861 taagaaaaga aacgtaacgc cgtttgtgac gttgcatcat tttgataccc ctaaacgttt
    16921 gtttgaccat ggagattttt tgaatcgaga aacaatagaa gcatttgttt cctatgccat
    16981 tttttgtttt catgaattca aagaagtcaa ggtgtggagt acctttaatg aaatctatcc
    17041 agttgcaacc aatcaatatt tgttaggtgt tttcccacca ggaatcaaat atgattttac
    17101 aaagatcgtc gcctgtcttc ataatatgat ggttgctcat gcccgtgtag tgaactattt
    17161 caaagaaaac gaacttccgg gtgaaattgg tgtagttcac tcattagaga cgaaatatgc
    17221 ggcaacagac gcgccggaag ataagcatgc tgccttttta gacgatgcat tgtcgatccg
    17281 tttcttatta gatgctacct atttaggcta ttattcaact gaaaccttga ctgctcttga
    17341 tgagatatgt gaagcaaatc aggccagcta tcattttcct gaagaagatt tcgttgaatt
    17401 gaaaaaagca tccacgcgta atgactactt agggatcaat cactaccaat gccattttgt
    17461 caaagcttac gatggagaaa atgcaatcca tcataacggt acgggtgaaa aagggacctc
    17521 tgtttataaa gtgaagggaa tcggagaacg tatttataaa gaaggaattc ctcgaactga
    17581 ttgggactgg ctgatttatc cagaaggtct atatgacttg ttgctgcgaa ttaaaagcga
    17641 ttacccacat tacaacaaaa tctatatcac tgagaacggg atggggtata aagatcagtt
    17701 tgaagatggc attattatgg atcagccgag aatcgactat ttgagagtct atttggaatc
    17761 cttaagcaag gcgataacag ccggggtaaa tgtgaagggg tattttttgt ggtcgcttat
    17821 ggatcttttc tcatggacaa atgggtataa caaaaggtat ggcttgtttt atgttgattt
    17881 tgaaacccag aagcgttacc caaaagaatc agcttactgg tacaagttgg tcagtgaaac
    17941 caaaacgatt atttaataag tgataatgga ggaagaaaag atgaataaag aggaattaca
    18001 aatgcttggt tttgagatcg ttgcatattc aggcgacgct cgcagtacat tattgaaatt
    18061 attgaaagaa gtaagacaag gaaactttga acatgttgat tcggcgttga aagaagccga
    18121 cgaaaattta acccttgcac ataattcgca aacaaaaatt ttagcggaag aagcatctgg
    18181 gaaagagatg gagatgggat tcatttttat ccatgggcag gatcatttaa tgaccacttt
    18241 attgctgaga gatctgattc aagactttat tgtactgtac agacagaatc aaggatagga
    18301 gaagaacgct atggatggaa tcattaaaca aatcgaaaaa gggaaaccat tttttgaaaa
    18361 aatctcacgt aatagttatt taggagccgt tcgtgacgga tttttaacag cgatgccagc
    18421 tattttattt tcaagcattt tcattttgat tgctgcggtt cctgagattt ttggttacca
    18481 atggcctgaa tctgtctcca catggctatg gaaagtctat aattattcga tgggtgttgt
    18541 agggttactc gtttcagcaa ccactgctcg ctgcttggct gattcgatga atcgtcgctt
    18601 accgaaaaac aaagtaatca acaccacctc tgtgatgctg gcctctgtgg taagtttcct
    18661 gttgttaagc gtctctccga ttgaaggtgg attttcaacg gaatatatgg ggacaaaggg
    18721 actactggct tcctttgtcg ctgcattttt aacagttaat ttatataagt tttgtgtgct
    18781 gaaagatatt accattaaaa tgcctaaaga agttcctgga acgatttcgc aaaccttccg
    18841 agatattttt cctttctctt tttcagtatt tgcggccgtt ttaattgatt taggcattcg
    18901 acaaatcttc caaatctcat ttgcagaagc aatcattacg gtgctgcagc cgctatttac
    18961 agcagctgac gggtacttag gaatcgctat tatctgggga gcgatggcgc tgttttggtt
    19021 tgtcggtgtt catggaccgt cgatcgtgga gccggcaatt gcggcaatta tctatgccaa
    19081 tgtggaaacc aatctgcaat tgttcaattc cggtgaacaa gcttctaaag tactgacggt
    19141 gggtttaggg aattttgtag gaactatggg aggaactggg gcaacattgg ttgttccttt
    19201 tatcttccta ctctttgcta agtctaagca gttgaaggca gttgggaaag cttcctttat
    19261 tccagtaagt ttcgctgtca acgaaccatt gctatttgcg gcacccatga ttttgaaccc
    19321 ttattttttt attccatttt tgttaacacc tgttgtaaac gtttgcctgt tcaagttttt
    19381 tgtcgatgta ttgggcatga acagctttat gtacgttttg ccttgggcaa ctccagcacc
    19441 gatcggtttg gttctaggaa cgggcatggg tatcttagct tttgttctag cagcggttct
    19501 aatcgtcgtt gacttcctga tctatctacc attttgcaaa gcatacgatg ctgtcttagt
    19561 agagaaagaa gcacaaaaag cagaggtgat cgcaaccgag gaaggtgctt ctgccgatgt
    19621 acgtgaggaa gtacctgtgg cagcgagtca agttatcagc gatgcaaata aagaaatcaa
    19681 tgtgttggta ctttgtgctg gcgcagggac aagtgcgatg ttagctaatg ctttaaaaaa
    19741 aggtgcatct gaatcgaaag caccgatcag tgcaatggcg ggtgcttatg gttctcatta
    19801 cgaaatgctg caggattttg atatggtggt cttagcaccg caagtcaatt cttactatga
    19861 tgatatcaaa gaagacaccg atcgcttagg aatcaagctg gttgcgacaa aaggagcaga
    19921 atatatcgga ttaacgagag atcctgaaaa agctgttcag tttattctga accaatttca
    19981 ataaaggagg agccataatg aaaatgggaa tttggttgcg taaagctgtt ttatttgcag
    20041 ctgtgttagg ggctttgggg gctgtttcaa aaactgttga agcatctgcc agcgaattta
    20101 ttcgcggagc agatatttcg atcctgtcag atatggaaaa atctggtgca aaatattatg
    20161 aagacgatat ttcaaaagat gccttagaga ttttaaaaaa taatggtgtc aactatgttc
    20221 gactgcgttt gtggcaggat ccttataata gtaatggaga aagttatgga gcagggacaa
    20281 atgatctgga aacgacgatt gaccttgcaa aacgagcaaa aaatctggga cttaaggtgc
    20341 tgctggattt tcactacagt gatttttggg ttgatcctgg gaaacagaat cttcctaaag
    20401 catggcaagg tctgaccttt gaagaaatga atacggcatt gtacgactat acaaagaacg
    20461 ttctgtcgga aatgaagcag ctggacgttt atcctgatat ggttcagatc ggcaatgaac
    20521 tgaatagcgg aatgctctgg ccttatggca agtcttgggg agaaggcggg ggagaatttg
    20581 atcgattggc tgcttttttg aagtctggta tccaagctgt tcgagacact caacctaaaa
    20641 caactccagt aatgctccac ttagctgatg gtggcgatac aggagcattt acttggtggt
    20701 ttgatgaaat cacgagtcga ggtgtgtcat ttgatttaat tggagtgtcg tattatccgt
    20761 attggcacgg ttcaatgaat gaacttcaaa ccaatatgaa tgcgatcagc caacggtatg
    20821 gtaaagaagt gatcgtagtt gaaacagctt atgggcatac aacagcgaat gccgatacta
    20881 tgccaaatgc ttttggtgaa gccgaagcag ctgcgggggg atatgaacca acaccgacag
    20941 gacaagcaga gtatttgctg gatcttgccg atcggattca ggcagtaccg aataatcgcg
    21001 gggcgggctt cttctattgg gaacctcttt ggtacaacgg aaatgtgagt tgggcaacac
    21061 aagcaggtat gaattatctc agtgttcaat ccactatggg aaacgaatgg gacaaccaag
    21121 cgatgtttga ttttgctgga aacgccttgc caagcttgcg tgcgtttaaa caagcagggg
    21181 cgcaaataaa tctagttaaa aatgcaagtt tcgaaagcga tggaccaacg accagtcctt
    21241 ccaattggca aacctggttt caacaaggaa ccgctaaggg tacgatcaaa acggaagcgg
    21301 gcactttttt tggggactac aaactaactt tttggcagga tcaagcatac gaggcatcag
    21361 cttatcaaaa tgttagtggc ataagcaaag gaacttacac attgagtgcc tatgtaatgt
    21421 ctgggggaga gtttgatact aatcagttat atatcaaaaa ttatggtgga gcggaactaa
    21481 atactgcggt tgttaaaaca agtgaatgga caaaaattac aatcaaagac attccaatta
    21541 caacaggtgt ttgtgaaatt ggggtttata gcaaggcttc aggaaatgct tggacgaacg
    21601 ttgattttgt ttcattggtg aaacaataga ctgcggatcg tttcgattca agatcggagg
    21661 ttcccttcag ccacatcgtt agcgtagaag gcaggaatcg aacctgcacc tcgcctttgc
    21721 caaaaataac cagaaacgat ttccagtcat ttttaccaaa acgagtaaag caccagactt
    21781 catctacact ttgtattatg ctaaagagag ttcaacgatt tcgtaagaag caatgaaaaa
    21841 attgcaaaaa gttgtgcaga taacataaag tttgtttaag aaagggtaac cttagaattc
    21901 cgtcgtgaat atgatttgag cacttagatg gatagaaaaa agttctcgga aaacagttcg
    21961 tttttcgaga acttcttttt atgataagaa tgaaacaaat taacttatat tatagtctct
    22021 tttggtaggg aaccatctta tttttaatga aaatcaagca ttttagctat tgaagaatgt
    22081 tgatttatag ggattccccc tccatattat gttggctctt tattcccact ctgttttcaa
    22141 cgtaaagtgt ataaaacaga atgcgatact atcaagggag ttaaaacaat atacagaaca
    22201 gtaaaacact ataaataaaa ataaaagtgc aataaaagtg caatttttgc acctaatcat
    22261 actctgttta tatatgaaaa ctagattctg aatccaatat ttggttaacg agaggcgttc
    22321 gaatttgaac gtcttttata tattactttc aaataaatat gaggtagaat actatctatt
    22381 ttctttttaa cccaattgtt ataatggaga gtgtaaaata ttttgtgtaa atagaaaaaa
    22441 ggaagtccct tctgtagaat agagttacca caacacattc acagaagaga ggacttccct
    22501 atgaacgatt ttactacaga aattctaaag actctagcga acaaaggcga tttgaatgaa
    22561 ttattccgtg tccatttgga gaaagcagtc aatacgcttc tcaaaacgga gttaacggct
    22621 ttcctagatt acgaaaagta tgatcgcatt ggttttaaca cgggtaattc tcgtaacggc
    22681 tcctatgacc gtacggtcaa gaccgagtat ggggaacttc atctccagat tccgcgcgac
    22741 cgcaatggtg agttcaagca acagactgtt cctgcttata gacggacgaa tgacacgtta
    22801 gaggagaccg tcattcacct cttccgaaaa ggtattacca tgtcggaaat cgcagacttg
    22861 attgagaaaa tgtatgggca ttactacacg ccccaaacca tgtccaatat aacaaaatca
    22921 tttacagaag aggtaacggc gtttaatggg cgggagcttc atgaccgtta tgctgctatt
    22981 tatatggacg caacgtatat tccgttaaag cggaaaaccg tcgccaagga agctattcat
    23041 atcgcagttg gcattcgccc ggacggatca aaggaagtat tgagctatgc gattgcaccg
    23101 actgaatcca tcacgatttg ggaggaaatt ttattggacc ttcaagagcg cggtttgaaa
    23161 aatgtcctcc tgttcatcac ggatggctta aaggggatgg taggagcgat tagtcggttc
    23221 tatcccaaag ctcgttttca acattgttgt gtacacgttt cccgtaatat cagtcataaa
    23281 gtgcgtgtcg atgatcgtaa ggaagtctgt gatgatttta aaatggtgta tcaagcctca
    23341 tctaaagagg tggcgttgga agcacgtggt acttttgcgg aaaaatggaa aaccagctat
    23401 ccaaaagtgg ttgaatcgat tctttcgaac gatcacttgc tcactttcta tgatttcccc
    23461 ttggccatac gcaagagtat ttattctacg aacttgattg aatcctttaa taaacaaatc
    23521 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
    23581 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
    23641 caggccgaag gcgagctcga acaaatgcta agccaactga ttgagaatta gaaacaatct
    23701 gcagtaggga agaatcattt acacaaaatt attgacgctc ccttataatg agaactttta
    23761 ctaacataat ttatgataaa cttactatat aatcatatta attaaatagt taggagatct
    23821 gcttatgttt atatctactc ttcaaattcg aaattttaga aattatgaat ccgaaatctt
    23881 tcgattcgat aatgagacaa atactattat aggtgagaat gattcaggga aaaccaatgc
    23941 tcttactgca ttgagaatct tattagatga tagttattac tatagtagta aaacactaaa
    24001 agaatcagat ttttttcatg gaattcaaaa tgggtggcaa gggcattgga ttattatctc
    24061 tgcgacattt gaaggaatca gcgaagaaga atttgacaat gagatttgtg cttccatttc
    24121 tttaaattct gaaagtcaaa caatcttaga agaattgatt tcaaatgcag ataaaggtgt
    24181 aggtagtatt tctctattta ttagacctaa caaagctatt agaaagcaat tatttgatat
    24241 ttccactgaa ttagactctc atcaatttaa cgaatttaga aattctattc gtctatccga
    24301 ctatgaattt tactacacat ctaaatcaaa tctaaatttc tgtatcaatg aaaattatga
    24361 taaattggta ggaagactca atgacaatat agctagtaat cctgaagaag acgatgaagc
    24421 tctgttgggc tcaagaattg acatgtcaga tattttcaag catatttcgg ttgtttatgt
    24481 tgacgctctt cgagatgttc ttagagaaat gaaaaacaat cgaaacccag tcaaaagaat
    24541 catggaaaca attgaatcaa aaatatcatc agataatgtt gagtcattaa aaacaattat
    24601 acagcaatta aatgagacta ttaccagtgt tcctgaaatt aggaaaattg gcgaaaatat
    24661 taatcggcaa ctaaatagca taattggttc agtgtattct cctaatcttc ttctttcctc
    24721 aactatgtca gatgatatgg gttctttagc aaaatttctt tcaatgaagc cagaacaaaa
    24781 tatcgactta gacttactag ggttaggaca tctaaatatg atttatttag ctttgaaaat
    24841 agtcgaattt gaagcgtgtc gttcaagaga actacttaat attatgttga ttgaagaacc
    24901 agaagctcat attcatcatc atattcaaaa aactctgttc gaaggattaa atcttcaaaa
    24961 aaactatact cagattctca tgaccacaca ttctgtacat ttagctgaag tgtctgaaat
    25021 atctcggatg aatgtattaa aatcgtttaa tggcaaatct atcgttatga atccagcaaa
    25081 taatctgaac tcatttgcta aagaaaagtt aaaaaagaac aatctaaatc taataaacgc
    25141 agtagagaga tatttagatg ttaaaagaaa tggattgctt ttttctaaag gtgttatctt
    25201 agttgaagga gatgctgaag aaatacttat tcctcaaata gtaaaaaaat tatttggtat
    25261 aaacttagac gaacttggaa taggtttgat taatgttggt agtacttcgt ttgaatatat
    25321 cgctagcttg tttgacgatg atagaataca gagaaagtgt gctattgtaa ctgatttaga
    25381 tgaacaggca atcccatcag aacatcgatt gtataaaagc actgcacaag agaaaggtca
    25441 agcaagaaaa gaaaaattat cctccctttt taatgaaaac aattgggtta attgttttta
    25501 tgctaataca acatttgaaa ttgagttttt agatgtgaat aaagaaaata ttctaactta
    25561 tgtcagtcca attatagatg acgcattcaa agataaaaaa acaataactg cttataaaga
    25621 agaaatacaa agtgaacaat atttagaaag aaatttagcg atgttaaaac tagccaatca
    25681 tgttggtaag gggtggtttg caattgagtt aggtgaaaaa attgactttt taatcaaaat
    25741 tcctaagtat attcttaatg ctattgcttt tgcatgtcag gaaatagtag atctctcaat
    25801 ttataaaaaa attctactcc atacaatcga cttgtacgag gaaactacag aattgtcagc
    25861 tttatcagct agtttaaaaa caagtgatac agctgatttg aatatagcaa taatttcttt
    25921 attagagtac tttggtaaag atgaggatgt tgtgtatcta atcaatcaga tagaaaatta
    25981 cacaaaaata ctttatgctg aggtttcaaa atgacaaata tcttcgaaaa aatcgtaaat
    26041 gatttaaatt tagaacaaaa agatgttgtg gaaagtgaag ataaccttta tgtaaatgca
    26101 tgtcctggga gtgggaaaac aagggtactt acaaggaaaa ttgcttacca agcaattaaa
    26161 cacgatggat cactaaaaag aataattgct atcacataca ctaatagagc tgctgaagaa
    26221 attaaggaac gcctaagttt acttagtata gatgatgata ttaatatttg ggtggggaca
    26281 atacatcagt tttgtcttga atttattatt tatcctttta aaatgaatct ctcaagagta
    26341 tcaaaaggat ttacaattat tgatgattat actcaaagag cttacgtcag ccaaattaat
    26401 aatatgctga atttaaatct caaaacgtat gaactgaaca aaattgattt gtcactttct
    26461 catgatttta aaattaatga agttcagtat actcaagttt ctgaaatgta tcacactatg
    26521 ttggaaaaaa ataaagaaat tgattttgat atgattttac tagtagcgta taaaatttta
    26581 actgtaaaca gtaacgttgc acgtaatatt gctagtaata taagggcgat ttatgtggat
    26641 gaatttcaag atactagaga attacaatac aatgtaatag ctaaattact tcaaaacaac
    26701 cctcaaattc agtcaatgtt tgttggagat attgaccaag caatttatgg atcattagat
    26761 ggtatagcta aatcagttga agaactagaa atgctaacta gtcacacttt tcaatcaaag
    26821 acgctacatg gctgttacag gtctaatcag agattagtag acttctactc taattttcag
    26881 agttgtcctt acgagattga aagcagaggg aataataaaa acgaccgtgg ttttataagt
    26941 tatgattgca aagttactaa ggaggattta cccaacataa taaaaaatat aataaaggaa
    27001 aagattgaaa gcggcattca tgaaaaagat atttgtgtac ttgctcctca acactatcct
    27061 ctgttttcac tgggtgagta tttaaagaaa gaattaccgt attgcaattt tgatgctata
    27121 aatatatcac caattaaggt aaataatcat agtttatttt tcaagttagc aattttgtac
    27181 tttactgaat caggtgaaaa ggttcgaaga agaaagctaa ttgcaaacga tatttta
//
LOCUS       DACECJ010000031        26931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.031, whole genome shotgun sequence.
ACCESSION   DACECJ010000031 DACECJ010000000
VERSION     DACECJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.031"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            68..138
                     /locus_tag="IWO09_001383"
     tRNA            68..138
                     /locus_tag="IWO09_001383"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            151..224
                     /locus_tag="IWO09_001384"
     tRNA            151..224
                     /locus_tag="IWO09_001384"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:185..187,aa:Ile,seq:gat)
     gene            236..307
                     /locus_tag="IWO09_001385"
     tRNA            236..307
                     /locus_tag="IWO09_001385"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:269..271,aa:Glu,seq:ttc)
     gene            573..1766
                     /locus_tag="IWO09_001386"
     CDS             573..1766
                     /locus_tag="IWO09_001386"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0263984.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1896..3368
                     /locus_tag="IWO09_001387"
     CDS             1896..3368
                     /locus_tag="IWO09_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0263985.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3451..4239
                     /locus_tag="IWO09_001388"
     CDS             3451..4239
                     /locus_tag="IWO09_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0263986.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4306..4974)
                     /locus_tag="IWO09_001389"
     CDS             complement(4306..4974)
                     /locus_tag="IWO09_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0263987.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5089..6801
                     /locus_tag="IWO09_001390"
     CDS             5089..6801
                     /locus_tag="IWO09_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263988.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6804..8573
                     /locus_tag="IWO09_001391"
     CDS             6804..8573
                     /locus_tag="IWO09_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263989.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8778..9344)
                     /locus_tag="IWO09_001392"
     CDS             complement(8778..9344)
                     /locus_tag="IWO09_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0263990.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9344..10300)
                     /locus_tag="IWO09_001393"
     CDS             complement(9344..10300)
                     /locus_tag="IWO09_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0263991.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10300..10956)
                     /locus_tag="IWO09_001394"
     CDS             complement(10300..10956)
                     /locus_tag="IWO09_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0263992.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11326..13344
                     /locus_tag="IWO09_001395"
     CDS             11326..13344
                     /locus_tag="IWO09_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0263993.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13771..16185
                     /locus_tag="IWO09_001396"
     CDS             13771..16185
                     /locus_tag="IWO09_001396"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0263994.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16533..18086
                     /locus_tag="IWO09_001397"
     CDS             16533..18086
                     /locus_tag="IWO09_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0263995.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18467..20251)
                     /locus_tag="IWO09_001398"
     CDS             complement(18467..20251)
                     /locus_tag="IWO09_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263996.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20266..21987)
                     /locus_tag="IWO09_001399"
     CDS             complement(20266..21987)
                     /locus_tag="IWO09_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0263997.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22087..22848
                     /locus_tag="IWO09_001400"
     CDS             22087..22848
                     /locus_tag="IWO09_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0263998.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23815..24083
                     /locus_tag="IWO09_001401"
                     /pseudo
     CDS             23815..24083
                     /locus_tag="IWO09_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24755..>26931
                     /locus_tag="IWO09_001402"
     CDS             24755..>26931
                     /locus_tag="IWO09_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0263999.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         8616 a         4734 c         5609 g         7972 t
ORIGIN      
        1 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
       61 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
      121 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
      181 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
      241 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
      301 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
      361 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
      421 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
      481 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
      541 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
      601 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
      661 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
      721 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
      781 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
      841 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
      901 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
      961 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     1021 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     1081 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     1141 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     1201 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     1261 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     1321 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     1381 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     1441 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     1501 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     1561 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     1621 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     1681 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     1741 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     1801 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     1861 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     1921 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     1981 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     2041 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     2101 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     2161 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     2221 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     2281 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     2341 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     2401 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     2461 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     2521 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     2581 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     2641 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     2701 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     2761 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     2821 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     2881 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     2941 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     3001 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     3061 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     3121 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     3181 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     3241 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     3301 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     3361 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     3421 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     3481 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     3541 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     3601 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     3661 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     3721 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     3781 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     3841 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     3901 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     3961 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     4021 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     4081 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     4141 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     4201 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     4261 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     4321 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     4381 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     4441 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     4501 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     4561 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     4621 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     4681 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     4741 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     4801 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     4861 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     4921 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     4981 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     5041 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     5101 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     5161 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     5221 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     5281 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     5341 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     5401 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     5461 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     5521 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     5581 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     5641 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     5701 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     5761 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     5821 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     5881 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     5941 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     6001 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     6061 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     6121 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     6181 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     6241 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     6301 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     6361 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     6421 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     6481 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     6541 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     6601 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     6661 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     6721 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     6781 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     6841 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     6901 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     6961 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     7021 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     7081 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     7141 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     7201 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     7261 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     7321 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     7381 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     7441 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     7501 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     7561 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     7621 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     7681 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     7741 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     7801 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     7861 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     7921 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     7981 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     8041 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     8101 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     8161 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     8221 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     8281 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     8341 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     8401 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     8461 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     8521 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     8581 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     8641 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
     8701 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
     8761 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
     8821 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
     8881 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
     8941 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
     9001 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
     9061 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
     9121 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
     9181 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
     9241 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
     9301 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
     9361 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
     9421 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
     9481 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
     9541 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
     9601 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
     9661 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
     9721 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
     9781 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
     9841 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
     9901 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
     9961 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    10021 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    10081 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    10141 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    10201 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    10261 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    10321 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    10381 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    10441 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    10501 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    10561 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    10621 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    10681 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    10741 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    10801 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    10861 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    10921 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    10981 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    11041 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    11101 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    11161 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    11221 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    11281 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    11341 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    11401 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    11461 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    11521 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    11581 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    11641 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    11701 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    11761 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    11821 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    11881 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    11941 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    12001 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    12061 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    12121 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    12181 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    12241 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    12301 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    12361 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    12421 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    12481 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    12541 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    12601 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    12661 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    12721 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    12781 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    12841 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    12901 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    12961 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    13021 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    13081 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    13141 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    13201 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    13261 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    13321 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    13381 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    13441 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    13501 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    13561 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    13621 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    13681 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    13741 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    13801 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    13861 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    13921 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    13981 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    14041 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    14101 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    14161 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    14221 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    14281 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    14341 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    14401 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    14461 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    14521 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    14581 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    14641 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    14701 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    14761 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    14821 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    14881 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    14941 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    15001 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    15061 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    15121 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    15181 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    15241 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    15301 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    15361 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    15421 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    15481 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    15541 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    15601 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    15661 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    15721 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    15781 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    15841 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    15901 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    15961 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    16021 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    16081 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    16141 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    16201 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    16261 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    16321 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    16381 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    16441 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    16501 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    16561 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    16621 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    16681 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    16741 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    16801 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    16861 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    16921 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    16981 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    17041 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    17101 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    17161 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    17221 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    17281 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    17341 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    17401 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    17461 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    17521 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    17581 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    17641 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    17701 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    17761 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    17821 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    17881 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    17941 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    18001 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    18061 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    18121 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    18181 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    18241 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    18301 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    18361 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    18421 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    18481 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    18541 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    18601 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    18661 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    18721 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    18781 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    18841 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    18901 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    18961 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    19021 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    19081 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    19141 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    19201 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    19261 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    19321 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    19381 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    19441 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    19501 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    19561 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    19621 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    19681 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    19741 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    19801 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    19861 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    19921 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    19981 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    20041 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    20101 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    20161 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    20221 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    20281 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    20341 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    20401 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    20461 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    20521 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    20581 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    20641 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    20701 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    20761 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    20821 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    20881 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    20941 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    21001 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    21061 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    21121 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    21181 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    21241 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    21301 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    21361 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    21421 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    21481 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    21541 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    21601 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    21661 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    21721 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    21781 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    21841 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    21901 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    21961 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    22021 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    22081 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    22141 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    22201 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    22261 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    22321 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    22381 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    22441 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    22501 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    22561 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    22621 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    22681 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    22741 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    22801 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    22861 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    22921 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    22981 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    23041 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    23101 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    23161 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    23221 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    23281 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    23341 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    23401 aaggttattg ttatttttat atattctttt taagaatgat tgttttttta tagaaatttt
    23461 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    23521 tgactggtag tgatttattt ttcgtcgttt tataaaagtg ttattttttg gaaaaattca
    23581 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta tgactaatat
    23641 aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg gaatcaatga
    23701 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
    23761 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
    23821 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
    23881 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
    23941 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
    24001 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
    24061 tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga gcaaaaagag
    24121 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
    24181 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
    24241 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
    24301 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
    24361 aaccaataca gggattcact gatgaaattc tagagtctga ccttggtatc aacgatttag
    24421 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
    24481 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
    24541 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
    24601 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
    24661 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
    24721 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
    24781 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
    24841 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
    24901 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
    24961 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
    25021 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
    25081 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
    25141 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
    25201 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
    25261 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
    25321 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
    25381 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
    25441 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
    25501 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
    25561 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
    25621 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
    25681 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
    25741 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
    25801 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
    25861 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
    25921 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
    25981 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
    26041 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
    26101 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
    26161 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
    26221 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
    26281 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
    26341 aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
    26401 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
    26461 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
    26521 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
    26581 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
    26641 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
    26701 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
    26761 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
    26821 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
    26881 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc t
//
LOCUS       DACECJ010000032        25315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.032, whole genome shotgun sequence.
ACCESSION   DACECJ010000032 DACECJ010000000
VERSION     DACECJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.032"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IWO09_001403"
     CDS             complement(<1..276)
                     /locus_tag="IWO09_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264000.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAI"
     gene            complement(786..1568)
                     /locus_tag="IWO09_001404"
     CDS             complement(786..1568)
                     /locus_tag="IWO09_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264001.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1633..2469)
                     /locus_tag="IWO09_001405"
     CDS             complement(1633..2469)
                     /locus_tag="IWO09_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0264002.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2487..3896)
                     /locus_tag="IWO09_001406"
     CDS             complement(2487..3896)
                     /locus_tag="IWO09_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0264003.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3910..5733)
                     /locus_tag="IWO09_001407"
     CDS             complement(3910..5733)
                     /locus_tag="IWO09_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0264004.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6576..6902)
                     /locus_tag="IWO09_001408"
     CDS             complement(6576..6902)
                     /locus_tag="IWO09_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264005.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6948..7310)
                     /locus_tag="IWO09_001409"
     CDS             complement(6948..7310)
                     /locus_tag="IWO09_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0264006.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7347..7562)
                     /locus_tag="IWO09_001410"
     CDS             complement(7347..7562)
                     /locus_tag="IWO09_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264007.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7689..8024
                     /locus_tag="IWO09_001411"
     CDS             7689..8024
                     /locus_tag="IWO09_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264008.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(8100..10097)
                     /locus_tag="IWO09_001412"
     CDS             complement(8100..10097)
                     /locus_tag="IWO09_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264009.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10195..10470)
                     /locus_tag="IWO09_001413"
     CDS             complement(10195..10470)
                     /locus_tag="IWO09_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0264010.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10702..11436
                     /locus_tag="IWO09_001414"
     CDS             10702..11436
                     /locus_tag="IWO09_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264011.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11475..12617
                     /locus_tag="IWO09_001415"
     CDS             11475..12617
                     /locus_tag="IWO09_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0264012.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12712..14103)
                     /gene="sufB"
                     /locus_tag="IWO09_001416"
     CDS             complement(12712..14103)
                     /gene="sufB"
                     /locus_tag="IWO09_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0264013.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14108..14578)
                     /locus_tag="IWO09_001417"
     CDS             complement(14108..14578)
                     /locus_tag="IWO09_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0264014.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14565..15800)
                     /locus_tag="IWO09_001418"
     CDS             complement(14565..15800)
                     /locus_tag="IWO09_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0264015.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15797..17083)
                     /gene="sufD"
                     /locus_tag="IWO09_001419"
     CDS             complement(15797..17083)
                     /gene="sufD"
                     /locus_tag="IWO09_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0264016.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(17096..17869)
                     /gene="sufC"
                     /locus_tag="IWO09_001420"
     CDS             complement(17096..17869)
                     /gene="sufC"
                     /locus_tag="IWO09_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0264017.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18066..18914)
                     /locus_tag="IWO09_001421"
     CDS             complement(18066..18914)
                     /locus_tag="IWO09_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0264018.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18938..19624)
                     /locus_tag="IWO09_001422"
     CDS             complement(18938..19624)
                     /locus_tag="IWO09_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0264019.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19617..20651)
                     /locus_tag="IWO09_001423"
     CDS             complement(19617..20651)
                     /locus_tag="IWO09_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0264020.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(21046..21384)
                     /locus_tag="IWO09_001424"
     CDS             complement(21046..21384)
                     /locus_tag="IWO09_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0264021.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21386..21742)
                     /locus_tag="IWO09_001425"
     CDS             complement(21386..21742)
                     /locus_tag="IWO09_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0264022.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21748..22935)
                     /locus_tag="IWO09_001426"
     CDS             complement(21748..22935)
                     /locus_tag="IWO09_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0264023.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(23126..23971)
                     /locus_tag="IWO09_001427"
     CDS             complement(23126..23971)
                     /locus_tag="IWO09_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0264024.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            24205..25170
                     /locus_tag="IWO09_001428"
     CDS             24205..25170
                     /locus_tag="IWO09_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0264025.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7416 a         5316 c         4279 g         8304 t
ORIGIN      
        1 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
       61 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
      121 ttcatctgct aaaagaacca cttgttccaa caatcgatcg gataaatatt tatagaaaat
      181 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
      241 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
      301 taaagttgct atataccctc ttattttagc ataccccctt ttataaaaag caaaaaaaaa
      361 aagaaagaaa cttattcttg ctaataaact acgcgaaagg agtctaacgt tgtttgatat
      421 aacttagact gatcgttaac ttatttttta tatttctaca tagttatctt tagaaccatt
      481 tcagatacat atgaaacaat tacatatgat tttttcataa aaaaacagta ctgtctagta
      541 gtataaaata tacaaatttc ttaaaaattt tttcactaaa atcactacct gaaacagtct
      601 aaaacactta tatatcaacg tttaatgaaa ctatctccgt ttcatttcag atacaaataa
      661 atatagtcgg gctccaaatc cgaaaaaaaa tacccccagt agcttatcct aagctactgg
      721 agatgtataa gtacggtcat ataacaacgg ggtagggtaa ataaatgaaa aagatggaag
      781 aatcattatt caataacttt tttaaatact tcgttgtaat cagttacctg tccgttctta
      841 aataccacag aataatcttt tttcccattt tcaagtaacc gaagtggaat gtctttacca
      901 acttgctctg tcactgcatt actgatacct gttagattag ctaataaatc tgataaagat
      961 tgagcatctg tagaaatctc tgtatcagta tttatcgtga ttcctttatc ttcatttaca
     1021 agcttccaag taggtggtat agctgtgtat gtttcgatga atgaatcaat tctttcatct
     1081 atcgtcttcc cagtatctac ttctgtctca ttactcttag atgaatcgat tctttcatcc
     1141 atcgtctgct ccgtaactgc ttctgtctca ttattctcac cggaaactct cttcacaccc
     1201 aaacttatat attgtccatc tttagtgtgc atagttaaaa ttcccacgct tttactaata
     1261 aattcactat aaccagttct ttcaatagag acttcaatgt atttcacact atcatactca
     1321 aaagaaaaat agacatgtaa aagcattttg ttgtttacat ctacataata atcctttgac
     1381 tctacttgag accttcctat acggaataca gctttactgt ctgatatctc tataccagca
     1441 tccttattat taatctgcca atctccaagt agctcttctg gcaagctagt agtttcttgt
     1501 gaacacttta ccacttcact ggtagaggtc gaaacattca cctccgcact gttagaagaa
     1561 ttttccaagg aatcaggagc cttagcacaa ccaacaagtc tttatatatt gtgcagccga
     1621 tattttcttc attcagtaca cccttgaaat atgcagtgtt agataaagca gttcgtcctg
     1681 atttgctgag gcacccatct cttcttgaac gtactccttg attttcaagg ccgcccgata
     1741 ctctttcgga aaacgctgct tcactgaagc cagcaaaaaa tcatccttct ccggcaactc
     1801 ttccttctta atcattcggg caataaaaaa ctgtaaatgc gtcacaaatc ttaagtaaag
     1861 catcgaatct ttatcaacct ttttttcggg aaacagatat cctagcatag ccaagatatc
     1921 ctttagtgct cttaccgaga gcatggtttc ttccatattt gcttgttcac tttgggcatt
     1981 tacaatgtga aacgcaatgt ttccagcctc ctcttctggc aactgaacat tcattttttt
     2041 gttgatcaat tggatagcat agcagccgat ctcaaattct tttgggtaat acttttgaat
     2101 ttcaatcagc aggcgatttt gaaaaacaat gcctttttga ctgcgttcaa tggcatgctt
     2161 caagtgatca accaaggtaa aaaatagact tttattcagc ttaaagtcaa acttggcgtt
     2221 ggcataatcg ataattcgct tgacgcaaat aattagatca tccggagcag cctcaaacag
     2281 tttaatgtaa tcctttttta gagcatcaga aagtgaatct aatttaaata ctttttggat
     2341 tttgtcagcc ggaaccaaat cgcctttctt tagttgaaat ccaatccctt tccccatcaa
     2401 aattacttct ttgccttgct tatctaagca aagaattaaa ctgttgttta acgttttaat
     2461 tactctcatt agattcaccg ttctctctaa gctaagccat ttcttgcgat tactttttga
     2521 taccaaaaga agctttcttt ttttattcgt tttaattctt taagattctc atcggttcgg
     2581 tcaacatata taaaaccata acgcttattg aagccttgat gggtactaac aacatcaatc
     2641 gctgaccaag gtaaataacc aaaaacatct acaccatctg ttaaagctgc ttgaatagcc
     2701 agaatatgtt tggctagata atcaattcga tcctggtcat taattcgtct atcttcctca
     2761 actatgtcgg gtgccccata gccattttca gtaattaaaa taggtaaatc atagcgatcg
     2821 tacaatttcc tcaatgttaa tcgtagtcca gtagggtcaa ctacccagcc atatggcgtt
     2881 ttatctacat aaggattttc agctgcccgg tagacacctt cttctcccaa catgatctgt
     2941 tgatcccccg ctctagcgga aacatctgat ccgtcatttt tgctggcagc gatggtggca
     3001 gttgaataat aattaatcgc aataaaatcg ggtttaccgg aggataaaat tgcctgatcc
     3061 tctgcctcaa ttttaggata taatccacga tcattcaaat agttttcaaa caaataattg
     3121 tattttcccc gaactgctac atctaagaaa gaccagcctc tcaatgtttc ccaattatgg
     3181 gctgcaattg catcttctgg actggaggta gcttgataca tagccgttaa attgattgct
     3241 ggaccgattt ttcctttaag attcaactga tgatacaagt gaattactct agcttgagct
     3301 aataaaacat ggtgattaat ttgaaagagt tctttttttg aaggcagctc tccacttggt
     3361 ggcaaaccaa tcgctccagg atgaaggatc atcgtgtttt gctcattaat tgtcagccaa
     3421 tagttcacct tgtcaccata ctcagtaagc aatacctgta catagtccac aaaataatcg
     3481 atagttgctc gatttaacca accgccctgc tcctctagaa aataaggtaa atcgaaatgg
     3541 tacatggtga caatcggttc gattccctga ttcaggcatt catcaattac ttgatgataa
     3601 aaagaaattc ccttttgatt ggttttatta ttttcaatat ccataattcg cgtccaagcg
     3661 atggaaaagc gaaaagcttt taagcctaat tctttaaaca gctgtatatc ttctttatag
     3721 tgatggtaaa aatccgaggc aacttcaaaa ttagctaccc cttctgtatg ctgatacttg
     3781 tcaataatag acggttgttt cccatcctcc tgataggcac cctctgcctg gtaggcacta
     3841 atggaggcac cccataaaaa cttctctgga aatgctgaaa gctgtttgta ttgcacaaaa
     3901 atcctctcct taactaattt gcatcaatgg ttcgtgttcc tttaagcttc cgcttgcttc
     3961 actaattcct gcgtaatttt cactgttggt cactaccaca atcactgtat catcgtagcc
     4021 agcgtttcta atggcagcga aatcggcgtt gattaataat tgtcctgctg acacctgatc
     4081 tcctgctttt acaaatactt caaagggttc cccttttaaa ttcacggtgt cgatccccac
     4141 atggatcaat aattcaatac cttctttaga acgtagacca atcgcatgtt tagattctaa
     4201 aatagtaatg acttctccct caacaggtga ataaatgctg ttttcttctg gatgaatggc
     4261 tacgcccttt cccaatagtc ccttagaaaa gacagcatcg ttcacttcag aaataggaca
     4321 cacggttcct tttaccggat tttcaacaat gactgcttcg gctattgccg tttgctctga
     4381 ttcatcagaa gtatcgtcaa atccaattag ataagcaatg ataaaggttg caacaaaggc
     4441 gattacacac gtaataattg cgtgaacaat attcattgga ttttctccaa taaaggctgg
     4501 taaggctgct aagccagggg aaacaaaggc atatctgact aaaccgctca agccagcata
     4561 caaaccagca attgcaccac caatcatgga agcaactaat ggttttttca gcttcaaggt
     4621 tactccatag agtgaaggct cagtaattcc cattaaagca gtgatcccag tggacacagc
     4681 tagctgcttc aactctttgt ttttagtttt cgcggctaca gcaaaggtag cacctgattg
     4741 agcaatatta gaagccagca ttcctggccc attgatcatt tcagaacccg tactgctcaa
     4801 ttgcatagta gcgattggcg tcattgccca agctgtaccc gtaaccacca aaaatggctg
     4861 caaaccaccc atcaaaaatg gaattaacca agctgctttg ccgttaatcc attcagcccc
     4921 cattgctata agatcgttca gcaacgttcc aaacggtcca atgacaacca acgctaaagg
     4981 ccccataatt aaaaatacca gcatcggctt agtaaagaat ttaataaacg aaggcgaaat
     5041 tcgatccgca aagggctcta cataggacat gatccaaaca gtaaagataa tcggtaaaac
     5101 tgatcccgca taatctgcag ctaataccgg aattcctaga aaagaaacgg catcgccagt
     5161 agaaaaaagc tgtgaaatat ttggatgaag caaaatgcca gcaacagtaa tcgccaaaat
     5221 tgggttgaca tcaaacttta tggctgcccc ataggctaat agtactggca taaaataaaa
     5281 ggctgcatca gcaatagtat tcaaaatgat gtaagtttga ctactgtctg atagtacacc
     5341 agtcaaagtc aagacggcca aaatggcttt taccatcccg gcacctgtta tagctggaat
     5401 catcggtgta aaggtggtag aaatgacact aatcaatctt gaaataatac tttcttgatt
     5461 gtctgaagag gggccctttt taattgaagt tcccacttcc ttttgaattt ccttgaaggc
     5521 tgtttggact tcattgccaa tcaccacttg gtattgtcca ccgctatcca ccacactaat
     5581 tacaccaagt gttttttcta aggctttcgt atcggctttc cttttatctt tgagttcaaa
     5641 acgcagtctg gtggcacaat gggttaagct gaccacattt tcttttcccc caacctgttg
     5701 caatacattt cttcctactt ctctgtaatc cattattctc tctccttaaa gcagtaaaaa
     5761 agaccaagtc aaaccacgcc ccaatcaaca ttgaagtgtt agtttgtctt ggtcttgcct
     5821 gcataatcag taacatgcca ataacgttat tttgctgtaa tccaataata gcacattatg
     5881 atagcgcaat caacagggaa tttaattttt tatcttttcg tttgaccgta tagggtaata
     5941 taacagttag atcacatcaa ctagggtata tctgcgtata cttactgcta aaataagtat
     6001 atctaatatt aactatacca gtaaaaccat tcctacataa ccagcgggaa attcccaaaa
     6061 tcgtgaaagc atcaaaaatg catataacgg atcacacaac ttcaacaatt ctgctcttgg
     6121 gaaaaattga tgaatttggt gagtaaagga tggaataaac agcattccaa caatataggc
     6181 aacaatagtg atacttgttt ttttggtaaa aatcagtaag caatagccaa tttcaatcat
     6241 tccgagatat aaaggtatcg atagaatgat ttgggctagt attcttaaaa attaactagc
     6301 aaatgtttaa actgatttat ttctaatcta catgtagtaa gtatttcaat tccaccaatt
     6361 atcaataaaa ataccaaaca tatccataat aattttttct ttttcatttc ttttatattg
     6421 ttataacagt gaataacata aaaattcccg acaatcattg taatattttt ttgtcacctg
     6481 tattagtatt taatttatag gaaactataa aaactcccta gtgaatttac acctttggga
     6541 gttcctgttt ggacggtatg cataatacat ttaaatcaat ttaacattaa cacctcagca
     6601 tatggtatct ccacatatat acggtctttc tttggatagt actcaatatt tatgttgata
     6661 gcacttccat tgtctaatat agctttagcc cggtatgtgt tatctccatt ttcatgagta
     6721 atcgtcacat catcacatga tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc
     6781 aatggttttg atgacttctc taatccagat gtacctgtca tttttgaaaa aataatacct
     6841 ataatagcca agacaacaat acctataatg acaaacttct tgttcatatt tactttattc
     6901 aactttttat ctcctatctc caaaataatt tgctcttatc cctcttatca gtacattgac
     6961 atccctaaaa gtacaccaaa gattgctcca gctacccctg ctatcatcaa aatcgtaccc
     7021 attttctcat ctttttctcg atataagtag cccataataa cagcaacagc accaaataca
     7081 acaggaataa atgctaacga tactaccata gaaatccaac caataatcaa atacagcttc
     7141 cctgtctctt gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt
     7201 ttctttactg tgtctgttcc ggttgtctca tctgtttctt gtagtgctcc acaaaactgg
     7261 caatacttac tttcatctgt tatttctttg ccacattttc tacaatacat gtcttctcct
     7321 cctaaaatca ttcaattgct tattagttaa ctgtttactt tattaaatac atcagaaaac
     7381 agctcattca ttggtacact taatatctca gaaagcatct tcatttgata gggtttagga
     7441 atcgttctcc ctgtttgcca cgaacttaca cattgctgtg taacattcat tttttttgct
     7501 aactccgttt gagaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc
     7561 atgattctcc cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat
     7621 ataaaaaact tcaaatacca ttacaaatac aacaacttgt tgtactatac tgagatgggg
     7681 gtgtattaat gaaacagaaa caatatgcaa aggtcattag agaaaagcga aaagctctcg
     7741 ggtggacaca aaaagaatta gctgaaaaaa tcttctctac acaacaggca gtagcaagat
     7801 gggaaaattc tgtaacagag cctaacctcg atagcttaac agctctttct agagccctag
     7861 gaacccctgt aagccatttt ctagacaatg ttgtcgtaga ttatgaggaa gagtttttgg
     7921 ctctctatcg ttctcttagc actgaagatg cagttcgaac aattgactat atgaagctat
     7981 taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga ttgaaaaata aataacccac
     8041 cgaactttaa cggtgggtgt attgagatga aataagatta agagctatgg ataacctgtc
     8101 tagtttaata caattgggtc ttttctcttc cttactctcc gtccatcatc tcttgttaca
     8161 cttgtaaatt gatacagagg aactatccca gctcctgata atatcaattc ttttattgtt
     8221 tcatctatcc ctgccccttt cgctaaatat attaaaaaac tactacttgc tttcacattg
     8281 tatagtttct taatatcctt agaagctcgt tcaagagtga atccttgttt aacaaagtag
     8341 cctctgtttt ttattaaagc atttttcacg ctgttatccc caacattacg taggcgttca
     8401 caaacagtgt taataagttc tttctctgtc attagatatg tcccttgcca cgataagccc
     8461 tgtaaaactt catgtgcttc atctaaaatc aattgttttt ccttgctgag ttctgatcgg
     8521 gaattttgag gaaatactct tttagcctct ttttcgttaa aacatatctt aacccattca
     8581 taagaaaaat attgatatgt aaaatgaatt ttttccagaa gtggaaggat atcttcattc
     8641 aatttttgaa tcacagaagt tgcattaaaa acgtctaatc gatagacatt gcttctctta
     8701 tgattaaagt ttcctaacgc tttctcttcc tttaacctat ttaggtattc gagcatcttc
     8761 aaaggaacat cttcatcatc taattttttt atcaatccta taaaagtcat aagtgatagt
     8821 aacttattac atttatctat gtcataagtt ttaccatagt aagaactcat ttttttagct
     8881 aattgttccc ccgacaaaat acaagtcaca ctttccttac tatcatcaaa aaaattaaca
     8941 tcagaaagca agacatcata gacacgtcgc atttcctcta gataattcct tattgtcctg
     9001 aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta cttttgattc ctctttcaat
     9061 tttcgaaaat gtgtaggata caagtctagg ttctctaata gccaattaaa tgtttctacc
     9121 cgactgtctt tctttaaaat tagcatcaca actccaataa aattttcact gatatcacaa
     9181 ttactggaat gacaataaaa aatggaatgc ccagttttac tggtaaaaat actagcagag
     9241 ggattatgat cgttgtgaaa cagacatctt aagtttttac ttgacttaag taatttattc
     9301 atatcaatgg ttaacaatct ttcataaatc tcattaaaag acatgtcgga ggaatcaaac
     9361 aggcagtctt taaaccactg tttcatttct tctttgtcgt cattttttat caacttaaca
     9421 aaatcggttt cattgactag ttcatactct ctccctgaat cactttgaca atttttagaa
     9481 catgtcatgg taaaatcaat cggaagctca gtaatatcta acatgttgtc ttcattaatt
     9541 attatcactg catctgactt atatcctcca aaaaacattc tattcgaagc atttacattg
     9601 tcatcacacc cggggatgtt ctttttcaag tattcgtaaa cagcaaccat tttaccatag
     9661 tcagtaattg cttcttttaa tagaaagaca atcttgtatt tttctagatt ttcagagtac
     9721 cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta aattgataga taaaaccttt
     9781 tctacgctta agtatctact atcatcttta ctcaaatcta ctaccttccc cttctcatct
     9841 ttaacagtgt tatcgacatc cagaataatt actctggttt ccacaaaact ctccttgtca
     9901 ttgcctttat tctttgtctt gcttggaata aatgattgtg atgaatttag tatctctaca
     9961 aactcatctc ttgtaacttc tctatagtta tctaaacacc tatctcttac atcagttttt
    10021 tctttttttg taactggctt ctctaataat ttttcttgat ctacataaat tggaatatta
    10081 atcattgatg tttccatttg tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata
    10141 cagataataa aaacaaactc tttattacta tatgttttag tcttatttgt ttactcaacc
    10201 tttataccgt tccatgaaac tatcaatatc agatttttta aatctaatga ccgtatcaat
    10261 ttttataaca ggtaacccgt attgagaaac atattttcta atcgttccta ctgatacacc
    10321 tagatattca cttgcctgct gatagtccat atactctaga gtgactccag aaataatgtc
    10381 ttctagcata ggctttagca ctgtaattat ctctttcact tgttgttcaa cttgtataga
    10441 aacaatgcta tcaatgattt gtagagacat atttccccct cctttcgtga ttgcttgaac
    10501 aaattttcta agaattatct tcgtattttt tgctcaaaat aaaagtattt tgttattaac
    10561 cgattaatca aacaaaaaat gattaccagt tcatcgacat acacataata cctcgataaa
    10621 aaaggggtgt caacagcttt gtaaaattta tttgcaacac cccttttttg aggtatacta
    10681 attttaggag gtgtggtcaa tatgataaaa cgtccaccaa taaattattt agaacgaaaa
    10741 aagatactcg gaacaaaaat aaaagctata agaaagagta aaaaattaac acaaccagca
    10801 tttggtctta tgattaacaa cggtcaatta attgacaaaa aaactatcta cgaatgggag
    10861 aagggaacct atttaccaat accagagcga ttatcccgaa ttgctgattt gggaaatatg
    10921 agtatagagg agttagtttg tggcaatgta gaagaatata ttttgggaat tattctttat
    10981 agagatagca ttgtactaga tggtataaca tttccagata aaaacctgtt ccagcatctt
    11041 cgtcaacagt tcccaccagt acactctaat ttagatacat ggctggatag atattccaaa
    11101 ttagaaccag agatgcaaga atttattgct aacaagactt gtaataaagt taaaaatgaa
    11161 aaaattagtc ttttcaatat cttaaaaata gaagaattat tcatcaatgc tattgtagaa
    11221 gaatttgaca ataacattct tttcctcact agcagtatag aagaactact agaaagaatg
    11281 gtagatgaat ggctacctat tcaattgaaa gacatgtctt atcccgaaga agctgtgaga
    11341 gaaattactg ataatataaa caagctagaa caaacgattt catcaattgg gaaaaaatac
    11401 actaagaaaa aaatgaaggg aggtgatacc atctagtggt aagtactcct tgtaaactaa
    11461 gaggagatga tttaatggca aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt
    11521 ggatgtataa aggacacatt gccaccgatc cacgaactgg tgaaaaaata aatactacga
    11581 gaagaggttt tagtagcaaa caagaagctc aaaaagatta tgacgagtac agacatcaaa
    11641 tattatacgg tgttaaaaag aaagcacctg atatgacatt tgaagacctg tataatgaat
    11701 ggataactca tcagcgtacc agtgttaaag cttcaacaat cgcaatctca gtaagatacg
    11761 caaataatca aatacttcca gcatttggta agcttaaact atcgaatatc tctgtcccat
    11821 attgtcaaaa agtggtagat gaatggcaca gtaaatatga aagttacgat tatatgagaa
    11881 aacagactgc acaaatttta cgctacgggg ttgcaatgca atatattgac aataatccta
    11941 tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa
    12001 aagaggagct taataattta ctggatgctt ttaaagattt tggtaacatg aaacaatacg
    12061 ccttcttcag attattggca tacacaggta tgagaaagag tgaagtactc gctttacagt
    12121 ggaaagacat agacacgttt aataaagaac tacatgtcaa taaaactctt gctgtagatg
    12181 aatttggtaa agttataatt caatctccga aaacaagagc ttctagacga gttatctctt
    12241 tagatactga gactctttct atcctcaaca actggaaatt acagcaaaaa gaagagtatt
    12301 taaaacttgg atacaatact agctctaaag agcaacatgt ttttacaaca gttaaaaaca
    12361 ctctatacat tccaaacact gttaatgatt ggttaagata tattttgaaa aaatataact
    12421 tgcctcgtat tacacctcat gggtttagac atacacatgc tagtttatta ttagaagcag
    12481 gagaatctgt caaggtagtt caacaacgat tagggcatga aaatagtaaa gtgacattag
    12541 acatctatgc acatattaca aataatgcac ctaagaaaac aggtcaagac tttgctgata
    12601 tgatggctca tcagtaaaag agctggtcaa aatctggtca tttctagtat acaggcaaaa
    12661 caaaaaagct agaaccctta tatatcaagg gttctagctc ctataacagg tttacccaac
    12721 actgccttcc atctcataac tgatcaatcg attgagttcg actgcatatt ccattggaag
    12781 ttctttggtg aatggttcaa caaagcccat gacgatcatt tcagttgctt cagattcggt
    12841 caggcctcgg ctcatcagat agtagagttg ttcttctgag atttttgaga ctttggcttc
    12901 gtgttctaaa gagacttggc tgttgtggat ctcattgaat gggatcgtat cggatttcga
    12961 ccattcgtcc atgatgatcg tgtcacattc gatatgagag atcgaacccg cactgttttt
    13021 ggcaaaggtt acttgtcctc gatagtttac ttctccgccg tcttttgaaa ttgatttaga
    13081 gacgatagag ctagatgtat ttggtgcatt gtggatcatt tttgcacccg tatcttggat
    13141 ttggtttgct cctgcaaagg cgatggagag catggtgcct cgtgcacctt ttccatctaa
    13201 gtaaacactt gggtatttca tcgttgtttt tgcacctagg tttccgtcga tccattcaac
    13261 ggttgcgcct tcgtatgctt ttgctcgttt ggttactaag ttgtacacat tgtctgacca
    13321 gttttggatc gttgtgtaac ggcaatacgc atcttttctt gtaaagattt ctacgatggc
    13381 ggcatgcagg ctgttacttg aataagtagg tgccgtacaa ccttcgacat agtggatgct
    13441 tgctccttcg tcaacaatga tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat
    13501 ccggaagtac gtttgtaatg gaacatcgac tttgacgcct tttggcacgt aaataaaggt
    13561 cccacctgac catacggctg aattcagcgc tgctaatttg ttgtcggttg gcggtacaag
    13621 ttttgaaaag tactctttga ataattcagg gtattctttc aaagcagagt cagtgtcagt
    13681 aaagacgatt cctaactttt cgaattcatc tttcatatta tggtacacga cttctgattc
    13741 atattgtgcg gaagcaccgg ctagataagc tcgttcagct tctggtatac caatcttttc
    13801 aaatgtatct ttgatttttt ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg
    13861 tttttggtaa tatttgatgg catcgaaatc gatgtctgaa aggtcggggc cccatgtttg
    13921 cattggcatt ttgttaaatg tttctagaga tttcagacgg aattcaagca tccattctgg
    13981 ttcgcctttg attcttgaca tctcacggat cacttcttct gtcaagcctt ctcctgtact
    14041 gaaaaccggt tcgacatcat cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc
    14101 catgttctca ctcctcttcc ttgtggtaat gatggaaagc tgcggtacca tcgttttcca
    14161 ttgcttttcc tagtgctttc caggcaagtg tcgcacattt gatccgagcg ggaaatttag
    14221 ctacaccacc aagcatagca gcatcgccta attggtcggg atctttgact tcttttcctt
    14281 ggacaagctg tagaaagtct acagttaaat gttctgcttc ttctattgat tttccgatga
    14341 cggcgtctgt catcatcgag gcacttgccg tactgatgga gcagccactg ccgtcaaagg
    14401 cgatattttt gatgattccg tcttttacat cgacttgcaa ttcgatcaca tctccacatg
    14461 tggggttgtt caattcgatc ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat
    14521 gggaagaatg atccaagatc acttgacggt ataaattgtc taatttagat agtcccatgt
    14581 tggaaaaact cctttgttgc catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg
    14641 ttgtagaaat aaaagctcgc gcgagcagtc gctgatactt caaggtactt caatagcggt
    14701 tgcgcacaat gatgacctgc tctgacggct accccttcca tgtctaaagc tgtcgcgaca
    14761 tcatggggat gtaacccatc caaattgaat gcaacgactc ctgtccgaaa attcgggtct
    14821 tgtggaccat aaacagtcaa tccatcaatt gcttgtaatt ttggcaacac ataagcggct
    14881 agttcttctt catatcgatg gatattgtcc ataccgagtt tttgcaaata atcgattgca
    14941 tggcctaatg cgatagcgcc ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt
    15001 tctttccatg tactgtcgta taattcgaca aaatcgatca tttcgccacc aaattcaaca
    15061 ggttccattt cttctaacca ttttcgttta ccatataaaa caccaatacc tgttggtccg
    15121 cacattttgt gtccgctgaa agcaaagaaa tcacagtcta gtgcttggac atcaatggcc
    15181 atgtgcgggg cagattgagc accatctact acgatgaccc cgccgttttc atggacaagt
    15241 tcagctaatt cttgaacagg attgaagaca cctaagacat tggatacatg agcaatggag
    15301 acgattttgg ttttgtccgt aatctttttc gctgcatcct ccatatccag gaagccttct
    15361 tctgttagtt cgatatattt caagacagca cctgttcgtt ttgccaactg ctgccacggg
    15421 ataacattag aatgatgttc catgtaagag agcactatct cgtctcctgc tttgatatag
    15481 cggtcgccga aggttctggc tacccaattc aagcttgtcg tcgtaccacg agtaaacagc
    15541 acttctgctg tttcttttgc atggatgaac gcccgtactt tttctcttgc ttgttcataa
    15601 tcatgggtcg cgcgttctgc taacgtatgg acaccgcgat gaacattggc gttatcgtgt
    15661 tcgtaatagt acgctaatgc ctggagtact tgttttggct tttgggtcgt cgctgcatta
    15721 tccagataaa ctaaaggttc atcattgacg tcttgaaata agataggaaa atcattgcgt
    15781 agctttttag catcgatcat ccattcaact tcccttcgat tacttcgact aattctttcc
    15841 gaacttgttc tacagggatt gctgttaaga cagaaccgag gaatcctcga atcactaagc
    15901 gctctgcttc ttctttatgc aatcctctac tcatcagata gtacatttct tctggatcaa
    15961 ctcgtcctac actggcagca tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag
    16021 gattcgcatc accacgtgct ttatcagaaa gcatcaagac acgactttct tgttgggcat
    16081 ctgcaccttt tgcccctttc aagatgtggc cgataccatt aaaggtcaat gttccttttt
    16141 ctcggatcac tccgtgttgc aaaatatgcc cgatcgtatg aggggcttta ttggttactc
    16201 gtgtatcgat tccttggatt tgtctgcccg agctgattgc cacgattttg acttctgcgt
    16261 gtgcaccttc tcctgctaaa tctgagtcaa agtcagcgat cacatgaccg tcattcatga
    16321 caccaattgc ccagtcgaca gaagcatctc gtaaaatgtg cccacggcga ttcatataag
    16381 aagtaatatt ttctcctaat tgatctacgg ctgaatattt gatttttgaa ccagctttag
    16441 cgatgacttc cacgatgata ttacctgatg attttgctac ttgttctttg gttgtctgga
    16501 aacgttccag ataagaaaat tcactatgtt catctgccac gatcaacaca tgcttgaaga
    16561 agtgctcatc ggatgcgcca tcttgaatga ataaggactc gagtggttca tcgatcacga
    16621 catttttagg cacatacagg aaaataccgc tgttcataaa agctacatgg agtgcagtca
    16681 attggtcttc gttcatgttt actgcttttt tcatataata ttcttggact agttctggat
    16741 attcgatcat tgcagtgaac aagtcggtaa aaatgactcc tttttctgct aattccacag
    16801 gcagttgttc aaaaatcgtc catgaacctt gttggacaag cacaggattg tctttcattg
    16861 catcaaatgc cggaatcgtg cctgtttcac tgatggtttg tgtttctttc acatcaaata
    16921 acgaccagcg atggaatttc acccgatcga tatgcggcaa tggcagttca tctgctttcg
    16981 ctagtgcgtc ttgacggaag gttctcatcc aatcaggttc gcctttactt aaagaaaaat
    17041 catttaccgc gtcaagataa tcgagccagt ttttttcttt cattcttgct cccccttacg
    17101 cttcttcttc tttatagtcg attcctaatt ctttactgat accagcgtag ccttctgctt
    17161 ccagacgttt agctaggtca gcattccctg tcatgactac tcggccttcc atcatgatgt
    17221 gaacgacatc aggtgtgata tagttcaata atcgttgata gtgggtgatg atcaatgcac
    17281 cgaaattgtc tccgcgcatt tcatttactc ctttagcaac gactttcaaa gcatcgatat
    17341 ccagacctga atcgatttcg tctaaaattg caaatgtagg ttctaacatc aacaattgca
    17401 ggatctcatt ccgtttcttt tctccgccag aaaaaccttc gttcagataa cgttcagcca
    17461 tttcttctgg catatttagt aaagccattt tttcatctaa ctttttcaaa aatgccataa
    17521 cagaaatttt gtcgttttca tcacgtttcg cattgatcgc tgcacgcatg aattccgcat
    17581 tcgtgatacc tggtatctca cttggatatt gcatagctaa aaataagcca agtcgcgcac
    17641 gttcatctac ttccaagtct aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta
    17701 cttcatagtt tggattgccc atgatagcgg cagataaggt agattttcct gtgccgttag
    17761 gtcccatgat ggcatggatt tctccggttt tcattgttag gtttacgcct ttaaggattt
    17821 ctttttcctc gatcgacaca tgtagatttt ttatttctaa gacagacatg cattataccc
    17881 ccaaattctc tttcgttctc actcatttta gactaattga gaatgaagcg caatgacctt
    17941 ccttactaaa atcaaatatc ttttatttat tattaaacaa ttttgatttc taaattagaa
    18001 taggtataaa aaagatgaga aataagccat tacagactta tttctcatct tttttgttcc
    18061 ataccttatt ttgcatcagc ctttactggt ttaacagagc cgccccattt gtctgtgatc
    18121 cattcttgga cttctttgcg gtgaagcact tcaatcagtt ttttgatttt gtcactgtct
    18181 tcatcacctt tacgagtcgc aataatattc acgtaaggag agttgtcctt ttcaagcaat
    18241 acggcatctt ctaatggatt caaccctgcg ccgtaagcga agtttgcgtt gattgcaact
    18301 aggtcacctt catcattttg gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg
    18361 aacttcaagt tcttagggtt ttcttcaata tcatcgaatg ttgctgtttc cagatctact
    18421 ccatctttga ctttgactaa gccagcatct tgtagaattg tgatgatgcg tccccagtct
    18481 gattcagaat ttgaagtaat gattgttgca ccatctttta attcggaaac atctttgact
    18541 tttttagagt acaagcccat tggttcgatg tggacagcac cggcattgac aaagtcatat
    18601 cctttttctt tgatttcttt gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg
    18661 tcgccttctt ccaaagattg gttcggtaat acatagtcat cgaatttgac gatttcaaga
    18721 tcgattcctt catctttcaa taatggtttc acatgttcca aaatctctgc gtgcggtgtc
    18781 ggtgaagcgc cgactttcaa tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg
    18841 ctgttgttac cgcatgctgc taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa
    18901 attttctttt tcatatccta catcccccta tatcctatta tcttttgtct gttttcttag
    18961 ttaagaaatc accgattcct tgaacgataa atacgattag taaaataatt aaagtcgcaa
    19021 cgaatgtcac tgttagatta ccacgctggt agccttcctg ccaagcaaga cctccaagtc
    19081 cacctgcgcc gatcgcgccg gccatcgcag taaatccgat catcgagatc gttgtaactg
    19141 ttaatccaga aatcaatgcg ggaatacttt caggtatcaa gaccttccag atgatttccc
    19201 agtaacttgc tcccattgca tctgctgctt ccagtacacc agggctgact tcacgaaaag
    19261 caatctctac tagacgggca taaaatggtg ctgcggataa aacaagagct ggaatggctg
    19321 cttgcgcacc tagcatagtg cctacgattg ctttagtaaa tggaatgatc aaaaccatca
    19381 ggatcataaa tggcgttgac cggaaaatat tactgatgat cgatacgatt ccataaatga
    19441 ttcgagcata cggtttgttt ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc
    19501 cgatgatcgt cactaagacc attgagatta atgtcatata tagtgtatca aggatagcct
    19561 gattcatcgt ttccgggttg atttgtttaa aatctagata tgtttctgaa aagcttttat
    19621 ccacgatgga tcacctcaat tcctactcct tgttctttga tgaacacttc tgcttgatct
    19681 aattctttgg tttcaccagt catcaagaca gtcaatgaac caaaggaacc ttctttcact
    19741 tgtttgatct ttccatacac aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa
    19801 atgactggct cgttggcatt tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga
    19861 ttttctttga tgagttcagc taatagttct gtcgaatcag cttctggttc gatgtcttgt
    19921 tgtacaaagc gtttggttac ttcttttttc ggttgtctga agacagtcaa tacgtctcct
    19981 tcttctacga ctttccccaa ctccatcaca gcgactttgt tgcagatttt gcggatcaca
    20041 ttcatttcat gtgtgatcaa gacgatggtc aaattcaatt tcttattgat cgctaacaat
    20101 aagtctaata cttcttctgt tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc
    20161 aggatctcag gatcatttgc caatgctcgt gcgataccga cgcgttgttt ctgtccgccg
    20221 gaaagttgtg aaggatatgc ttgtccgcgt ccttctaacc cgactaaacg caacagttct
    20281 tctgcccgtt ctttacgttt gctctttggt acgccagcta attccagcgg cagctggata
    20341 ttttcaagta cagttcttga ccataacagg ttgaaatgct ggaagatcat accgattttc
    20401 tttcggaatg tacgtaattc tttgtttttt aattttgtaa tatcttgatc gtttacgatc
    20461 acttcgccgc tcgtgggaag ctcaagtcca ttcaggagac ggactaatgt actttttcct
    20521 gcgccagaat agccgacgat tccataaata tccccttttt caacgtttag tgaaacgtca
    20581 ttgacggctt ggatgtttcc gtttttccca ttgaatgtct ttttgacatt cgttagttgg
    20641 attaatgaca tgccattcac atctttctct tatgattttt caaacaaaaa agccttcgcc
    20701 ctcttgcctt aagcaaaagg acgaaagctt gaacttccgt gttaccacct tgatttaccg
    20761 ttacttcgca ataacagcct cttccagtac ttggacttag cttctttccg tatactgtcg
    20821 cgctgtaacg ggcactcccg tagtaagttt gcttacgctc gcttactctg ctctaagacc
    20881 atcttccatc tcattttcgt tacccatttt cagcaaacag ggctctcttc aaacgtccgt
    20941 gacatgtact cttcttttca aagctttctc gtttcatata ggattgctta tgattatagc
    21001 aactggaaga atcagtgtca acttttttca agtgattttc tgattttata attcgtcaaa
    21061 ttcttttgga tcaacatctt tgaaacttaa gatccacgca ttcatttcat ctgaatcatt
    21121 caagatattg atatcttcac cgattttatc attaacagaa gaaactgtac ccgttagcgg
    21181 gctggcaaat tcattgaccg ctttttccgc ttcaacttcg atcagaggtt catctttttt
    21241 tagcgattgt cctgcttttg gcagattggc aaacgtgatg tctccaagtt cttcttgcgc
    21301 ttcatttgtc aaaccaacac agtattcttt tccattaaat aagatccaaa ggttgtcctt
    21361 tttcttcaaa tgcttgcctg ccattttata tcagttctcc ttcataaatt ttttcattga
    21421 atccaattgc aacaagctgg tcctctcgta agatcagtgg tcgcttgatc agcataccat
    21481 ctgtcgataa gacttggctc gcttttgaag ccgtaaactc gcctacttga tctttcagtc
    21541 caagttcccg atatttcatc ccgcttgtgt tgaacaggcg acggattggt acttgactcg
    21601 tttctagcca agtctcaatc gttgaggcct tcggtacttc tttgatcata tcaacggttt
    21661 gataactaac gccatgctgg tctaaccacg ctttggcttt tttgcatgtt gaacatttcg
    21721 ggtaccagta aaaggtgtac aattttctta cctccttgaa gatcttgcag gtgcttcatt
    21781 ttggtatctc atcgataata tcaacccgat accgatcatg ttccctaaaa tcgaggatcc
    21841 tccctgacta atgaaaggca atgggatccc tgttaaagga agtaatccga tatttgctcc
    21901 aatattctca aagacatgga acagcagcat catgatcaaa ccgcttgcga tataggcgta
    21961 aaattcatta ttcgtatcga aacacacccg aatcatacga tagattaaaa taaaatacaa
    22021 gaaaatgacg aatgcaccac cgataaagcc gaagttttca ccgataacgg taaaaatcat
    22081 atcggactca cgaacaggca catagatatt gcttacgttg aatcctttac caaacaagcc
    22141 accggtacca attgccaaaa gtccttgggc cggctgataa ctcattcctg acgtatcatg
    22201 gaaagggtcc agccatgaat caatacgtgc aaactgataa gatttgaatc caactttata
    22261 aagcaagtct cttccgcctt cagtcgtaac caagaagatc gttcctgctc caacgactac
    22321 tgccaagatt actacaggtg cgatgatgcg ccaagagatt ccagacatca ggaaaatccc
    22381 gccaaagatc gcaagaaaga caagcatcgt tccaaagtca tcttgggcca tgatcaatgc
    22441 taaaaccggt attgtcacga tcaagagctt cccaatcagc caaaagtccg atttcatcgt
    22501 ccgttctttt acttgcgtat tatgtttagt tacgatcagt gccatcatca agatgtacgc
    22561 aatcttcatc aactctgctg gctggaaagt aaacgcaccg aatcgaaacc agtttttaga
    22621 ccctgttgaa gcagctaaat tctgatcata gaatttgagc aagagtccca ttaccactaa
    22681 ccccaaacca taaaggtaag gagtcagttt ccagatccat ttggaattca aatgcatgat
    22741 gacaacgatt gctgccgctc caactaaata ccagacaact tgcatcccta cgcctttcaa
    22801 gactgctgga ttgttcggat catggctaag tgctacatat aatgagagca gaccaatcaa
    22861 acataacaga aagactggta aaatcacgcc ataatcaata cgattatcgt tcgaagcttt
    22921 taattttttt tccatgtttc atcccttttc ttaaaaacca tactgctgat tattatagcg
    22981 gtccagttag ataatggaaa gtctttccat agacatttgg gaaaaattaa ctttccgttt
    23041 gtgcagttgc ttgagcaaat gcagtacaaa agcctactgc ttatctttag tgtatcatag
    23101 aacttttttt gatacatgca attatctacc agctgagatt ctcctcgctg tgagaagttt
    23161 ctgtgctttc attgtatcga aggatctctt cggcaatctc tagcagctga tcagaaacca
    23221 gattggttcg ttggatgaaa taaaaatatc gatggaaatt caacggagtc attggcacat
    23281 tttctggaac agagcgctta gacaaaatcg agcgaccgat tccttccttc agcaacgcaa
    23341 caattacatc gttacttttc acgatcatct tcttatcttg gatattcatc tcttcaaaat
    23401 aacgctgcgt gtaatgaaaa acaccagagt ttgcttctct tacaagccac aatttgcttt
    23461 ttgggtcccc agcgatgact agctggtctt ccatcaaaga gtatctttgt acgccggaag
    23521 tctctaatgg cttttcgata aaaccaaaat cggattcatg tttcgctact tgttctaatg
    23581 attcatgaga attcgtgatt tcgatctcaa aatgcacttt aggaaacctt ttgatcagat
    23641 gtttcatcaa ctgaggcaaa agatagacag caaaggtgtg ggaagcaacg atccgacata
    23701 cttcttcttc attttgttca ttgagcaatt tttgctgagt ttcttcccat tcttctaaca
    23761 aaaccagtgt ccgttcatat aataagtcgg cttgcttagt tggaagcatc tcttttcttc
    23821 cgttacgttg aaaaagatcg atattcaatt cccgctccaa ctgtttgatt tgattcgaga
    23881 ctgctggttg agataaaaac agtacttctg ctgctctgga aaaatttctt gtttcatata
    23941 ctgctcgaaa agtcgttaat aacttaaaca tggcaccact tccataactt tttatgataa
    24001 ttactataaa aactatttga tttaaatata caaaaaacca tctataatag aaatgtacaa
    24061 caaaacagtt gctgatccaa ctagtaactt agatgaagac aatcagctgt ctgcttgctc
    24121 gtaatcatac attcctattc attttacaat gtattcgttc atcttggtac taaaatctat
    24181 taaataaaaa ggagaatatt atatatgaag aactttttga aaattattcc gatcccgatc
    24241 tgcgggttga tccttgggtt agcttccctt ggaaatctgt ttaaagacta tcaccatgtt
    24301 gctttaggca atctaactgg cggtatctct atgattctta tgatattcat ttttgttaaa
    24361 ttgtttatct tattcgaaca cacgaaacaa acgttgcatg atccaatcat cgcctccgtt
    24421 tcaccaactt ttacgatgtc actcatggtt atttgtactt attttgtttc atatgaaagt
    24481 attgcaccaa ttgtcaaatt catttggctg tgcgctgtta tcttccaagt gatccttgtc
    24541 ttttatttca attatcacca tgttatcaaa gctgatcttt caattgaagc aatttatcca
    24601 agctggttta tcatttacgt tggatttggg gtcatcactg taactgccgg gaacttcttc
    24661 ccacttattg gaaaaatttt cttttggatt tcattgatct gctatgcgtt cttattgccg
    24721 atcatcatca aacgtgtctt tttcgtaaga aatatggctc atccgacacg tccgctgatt
    24781 acgatcgttg cagcacctgg ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa
    24841 cctaattttt atcttgtcgt agcattgttc gctttatctc aagtattgta tttcgttgtg
    24901 ctgtctatgc ttccacgttt gttcaagctt cgtttttatc caagctatgc agcctttaca
    24961 ttcccgcttg ttatctctgc aacagctata tttactactg ttcattactt caacagttta
    25021 ggtatctata gtagcgtatt ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta
    25081 gttgtcttct acgttcttgc tcattacgta cgtttcttaa ttaaagaaca tagcaaacat
    25141 ggaaaatttg tttctaaaaa aatcgcttaa taatcgattc aataggttgt gaaaaagctg
    25201 ttctgcatca agtaataaga acggccaaac aaaaacagct gttcatgttt ttgtactggt
    25261 tgggcttatt accgcagatg caagcttttg aacaccgttt accaagaggt cgtaa
//
LOCUS       DACECJ010000033        25194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.033, whole genome shotgun sequence.
ACCESSION   DACECJ010000033 DACECJ010000000
VERSION     DACECJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.033"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..746
                     /locus_tag="IWO09_001429"
     CDS             99..746
                     /locus_tag="IWO09_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0264026.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IWO09_001430"
     CDS             1139..3784
                     /locus_tag="IWO09_001430"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0264027.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IWO09_001431"
     CDS             4094..5407
                     /locus_tag="IWO09_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0264028.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IWO09_001432"
     CDS             5397..6050
                     /locus_tag="IWO09_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0264029.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="IWO09_001433"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="IWO09_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="IWO09_001434"
     CDS             6787..7872
                     /locus_tag="IWO09_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0264030.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="IWO09_001435"
     CDS             7869..9761
                     /locus_tag="IWO09_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0264031.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="IWO09_001436"
     CDS             9768..10145
                     /locus_tag="IWO09_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0264032.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="IWO09_001437"
     CDS             10299..11081
                     /locus_tag="IWO09_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAQ0264033.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="IWO09_001438"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="IWO09_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAQ0264034.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12083)
                     /locus_tag="IWO09_001439"
                     /pseudo
     CDS             complement(11996..12083)
                     /locus_tag="IWO09_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            12447..13109
                     /locus_tag="IWO09_001440"
     CDS             12447..13109
                     /locus_tag="IWO09_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0264035.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="IWO09_001441"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="IWO09_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13637..13837
                     /locus_tag="IWO09_001442"
     CDS             13637..13837
                     /locus_tag="IWO09_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0264036.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="IWO09_001443"
     CDS             complement(14091..15770)
                     /locus_tag="IWO09_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0264037.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="IWO09_001444"
     CDS             complement(15772..15984)
                     /locus_tag="IWO09_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0264038.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="IWO09_001445"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="IWO09_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0264039.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="IWO09_001446"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="IWO09_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0264040.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="IWO09_001447"
     CDS             19959..20156
                     /locus_tag="IWO09_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264041.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="IWO09_001448"
     CDS             20309..20794
                     /locus_tag="IWO09_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0264042.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="IWO09_001449"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="IWO09_001449"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0264043.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="IWO09_001450"
     CDS             21671..22198
                     /locus_tag="IWO09_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0264044.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="IWO09_001451"
     CDS             22289..23026
                     /locus_tag="IWO09_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0264045.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="IWO09_001452"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="IWO09_001452"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0264046.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="IWO09_001453"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="IWO09_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0264047.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8390 a         4103 c         5297 g         7404 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DACECJ010000034        24879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.034, whole genome shotgun sequence.
ACCESSION   DACECJ010000034 DACECJ010000000
VERSION     DACECJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.034"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(144..914)
                     /locus_tag="IWO09_001454"
     CDS             complement(144..914)
                     /locus_tag="IWO09_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0264048.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(907..1842)
                     /locus_tag="IWO09_001455"
     CDS             complement(907..1842)
                     /locus_tag="IWO09_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264049.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2158..3807)
                     /locus_tag="IWO09_001456"
     CDS             complement(2158..3807)
                     /locus_tag="IWO09_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0264050.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(3924..6527)
                     /locus_tag="IWO09_001457"
     CDS             complement(3924..6527)
                     /locus_tag="IWO09_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0264051.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(6539..7588)
                     /locus_tag="IWO09_001458"
     CDS             complement(6539..7588)
                     /locus_tag="IWO09_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAQ0264052.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(7614..7928)
                     /locus_tag="IWO09_001459"
     CDS             complement(7614..7928)
                     /locus_tag="IWO09_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0264053.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(7944..8390)
                     /locus_tag="IWO09_001460"
     CDS             complement(7944..8390)
                     /locus_tag="IWO09_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0264054.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(8391..10367)
                     /locus_tag="IWO09_001461"
     CDS             complement(8391..10367)
                     /locus_tag="IWO09_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ0264055.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(10525..11421)
                     /locus_tag="IWO09_001462"
     CDS             complement(10525..11421)
                     /locus_tag="IWO09_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0264056.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(11414..12094)
                     /locus_tag="IWO09_001463"
     CDS             complement(11414..12094)
                     /locus_tag="IWO09_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0264057.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(12095..12877)
                     /locus_tag="IWO09_001464"
     CDS             complement(12095..12877)
                     /locus_tag="IWO09_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0264058.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12870..13787)
                     /locus_tag="IWO09_001465"
     CDS             complement(12870..13787)
                     /locus_tag="IWO09_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0264059.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13951..14274)
                     /locus_tag="IWO09_001466"
     CDS             complement(13951..14274)
                     /locus_tag="IWO09_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0264060.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            15062..18289
                     /gene="fss3"
                     /locus_tag="IWO09_001467"
     CDS             15062..18289
                     /gene="fss3"
                     /locus_tag="IWO09_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0264061.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18402..18716
                     /locus_tag="IWO09_001468"
     CDS             18402..18716
                     /locus_tag="IWO09_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0264062.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18729..19103
                     /locus_tag="IWO09_001469"
     CDS             18729..19103
                     /locus_tag="IWO09_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0264063.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            19113..19448
                     /locus_tag="IWO09_001470"
     CDS             19113..19448
                     /locus_tag="IWO09_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0264064.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            19531..20871
                     /locus_tag="IWO09_001471"
     CDS             19531..20871
                     /locus_tag="IWO09_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0264065.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            20949..21623
                     /locus_tag="IWO09_001472"
     CDS             20949..21623
                     /locus_tag="IWO09_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0264066.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            21856..22290
                     /gene="dcm"
                     /locus_tag="IWO09_001473"
     CDS             21856..22290
                     /gene="dcm"
                     /locus_tag="IWO09_001473"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0264067.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            22291..22998
                     /locus_tag="IWO09_001474"
     CDS             22291..22998
                     /locus_tag="IWO09_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0264068.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            22988..23278
                     /locus_tag="IWO09_001475"
     CDS             22988..23278
                     /locus_tag="IWO09_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264069.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            23537..24721
                     /locus_tag="IWO09_001476"
     CDS             23537..24721
                     /locus_tag="IWO09_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0264070.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            24736..24855
                     /locus_tag="IWO09_001477"
     CDS             24736..24855
                     /locus_tag="IWO09_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0264071.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         7739 a         4795 c         4641 g         7704 t
ORIGIN      
        1 agctttttca caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc
       61 aacaaacgac aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa
      121 acgctgattt cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac
      181 caagtaacgg cgtacaaaac aacgatgaat agcacaagta cgatcatcga tgttgctact
      241 ggcacatcga ttgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga
      301 ttgactttcg aaacagcttg ccaaaatggc ggcaacatag agatggcata gaaaacgcca
      361 cctagataag ttaacggctg caagacaaat gtaggcacga tcgatacatc gtcataagac
      421 tgagcaaaaa tcccgttgag cagtcctgct aacgaaaaca agattgccgt catcaataat
      481 gtaatgatga ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc
      541 gttacgagag agccgacgag aatactgcga cccactccgc caatcacaaa gccccaaatg
      601 atcacatgcg taggaacagg cgctaccaag atttcttcga tgtttttctg gaatttttga
      661 gagaagaagg acgaagaaac gttggcatac gaactagtga tagcagacat cataatcaaa
      721 ccaggcacga tgaattccat atatgaaaaa ccacccatgt caccgatccg accaccgatc
      781 atttttccga aaataacaaa gtacaaggat gtcgtgatca caggcggaac cagtgtttgg
      841 acccagatac gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa
      901 taaagattaa acatttttct cctccgtatg attattttct tctgtgattt tcaaaaacag
      961 ttcttctaaa cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtcag
     1021 ctgatcgaag attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgttc
     1081 atcttcaaat gtacaagcgt atccttcaat cacaggtttg gtctcatatg gcgctaaatc
     1141 aaagataaat gtttcaaatt gcagtttgga tagaagctct ttcatactag tattctcgat
     1201 cagttcacct gattggatga tcccgatgtt gcgacaaagc atttcagctt cttctaaata
     1261 atgcgtcgtc aaaatgatcg ttgtaccatt ttcgttcaat tctcggagaa actcccacat
     1321 ctcgcgtctc aattcaatat cgactcctgc tgttggttca tccaagatca gcaaacgtgg
     1381 ttcatgcatc aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg
     1441 tgcacggaca tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt
     1501 catcgcttct ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac
     1561 cgtttcaaag ggattgaaat tgaactcttg cgggactaat ccaatctgtt gctttgctcg
     1621 gactagatcc gtatccagat cataaccgaa gacattcact tttccagatg ttttattgac
     1681 taaagaagta atgattccga tcgttgtaga cttcccagct ccatttggtc ctaatagcgc
     1741 atagaaatct ccttctttca cggaaagatc gattcctcgc aatgcttcga ccccacttcc
     1801 ataaaccttt ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta
     1861 tagacatgat tctttattgt atcatggaat acagcagatg aaaaaacaac ttggttccag
     1921 cgaaaaatca ggttgctggg ctcaggaaaa tctcctaatg aaagacatca atcggacgaa
     1981 gtcagacaac cttttcagaa aaaacaaatc gaagataaag gattactatt catatgtgca
     2041 acaaaaggag cgtgagacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
     2101 aacggcggaa gcaaaagcca agatttttga tccaataaat actttcgatc acttcgccta
     2161 tcccaataca tgccactgcc gcttttcatc aaataacgcg gcttccgttt ttgcggtttc
     2221 tactaaagtc tctgggtaat ccaaaacttt cagcaaggca atcgcatttc gggtccttga
     2281 aggtccttct tttaatttga aatcaaaact gattcctttt ccttctgtta cttgttcttc
     2341 aaagtggaca ttctcacaat aattctttaa aatctctgtc aattcgatat catgtgtggc
     2401 gacaaatgct aaagatggat actccgcaag ccaccgaacg actgaagcgg aagaagcaat
     2461 ccgttcgatc gtatttgtcc cttttaaaat ctcatctacg aagcagtagc agcgttcttt
     2521 ggaagccact tggtccaaca aacgtttgat cgatttgatt tctgaaacaa aataactgtc
     2581 gccttcaaat aaatcatctt cgattgccat ggaagtcaac acatgtccag gttgcagggt
     2641 aaactgttct gcaatcgctg tttggatcgt ctgtgctaga atacagctga tggctatact
     2701 tttgacatac gtagattttc ctgatgcatt ggaacccgtg actaatgtat tcttctgcca
     2761 gttgaccgga ttcactaccg cttcttttaa caatggatga tagatatctt tcgctgtcac
     2821 accgccctca gtaaactctg gtcggcaagt gatgggcata taagtacggt aattcaatat
     2881 tgcagcggct acttctaatt ctcccagcaa ttcccacagc ttgatggctt cagttgaatg
     2941 cttttctaat ttagtgagaa cgaaatgata ggagataaat ggcagcatga acatgatgtt
     3001 gacatattca aaaatcaact ctgcttcact cccactcttc atacgaaatg aaaaacctaa
     3061 tttagggatg tctcttaatg gtttagtttg ctgtttcagc tcttcttgaa gtggtgtcgg
     3121 cagttttgct agctgattgg cggctgaaat cgttgaaacc aaataactca tgctattcag
     3181 ctctgtttct agtgtttgtt tcttcactag ataatagacc gtattgaata ctaacccacc
     3241 gattgccaaa tagattccta gtacttgtcc taaaagaagc aaacccaaac ccaccagcgg
     3301 aagcaagcca agaaaaataa ataaacctac gcgtccaatc tttttacttt ttccatccgc
     3361 tagatattgt tttgcaaaat tatgatcttt ttttcctaaa cgagcgaatt gatattggat
     3421 tttttctctc agttgaggat tttcctgata aaaagcaatc agtttttcta actgctgatc
     3481 ttcaccaaaa tcaaagctgc gtaaacgttg atacaacgct tctgaaccta cactagaata
     3541 ggtactgttg attccttcga atacttcgaa catatccagg tcataccaag taagatcatc
     3601 gatttcgcta tcccatgaac ggaacttctt ctccgtgagc catgcttcct tcaagctttc
     3661 ttctttatca aatctcggtt gataaggaat tttttcccat ttgctacgaa ctgtttcctt
     3721 taatttcaaa cgtgaaaaaa tgtctaaagc aactaataaa ataacagctg caataataat
     3781 taaaacgata aattctcttt gacccatatc cctctccgtt cctttatttg ttagctttag
     3841 tatagcactg ttgcgctatt tgaaaagagg gtaaaaaaac acctttcaaa ataaaaggtg
     3901 tttttttctt gtatattaca tatttatctc tcatttatca aaattgacaa ataatcatag
     3961 ggaggaattg ttgttaattc ttgtttattc tccagtgcat tgacaactgt cccaagggaa
     4021 aggatagcct caggtactat tttttcttgt tctgttggat tagctaagcg aagaacaaat
     4081 ccttgttcat gaaaataaga aggataaatc gatgatacaa ttaagtcttc aggtaattca
     4141 atcaatgaaa atttacgagg aacatcaagt tcattttgta atggccaaat cttgttatct
     4201 aacctgtgaa taaatacatt cagtttctgc ttttgataag aaatagattg ttctaaccgt
     4261 ttttctgcct gttttgccac tttgaactca ttaaatcttc cttcttctac attaaatcca
     4321 aaagtgactt tatactcacc tatctcttga gctagcggtg tttccatcat gacatgccct
     4381 tcatttgtcg tatctcctga agctcgccca ggtcgccaag caagattagg ttttccaagt
     4441 tcccctgttg ttgaaaataa ggttatgatt aattgatttt ctatacgttc atactctttt
     4501 aaaccatctg cataaaacgt aaaataatga tcattttctg ccacagagac actcttatcg
     4561 aatatttcta aattaacagg tttctctacc atagtttctg accaattcgg aggaatgaca
     4621 tcaggggtat tttcaataaa tccgttttga atttgtgcaa ttgatttttc agatttatct
     4681 aaaacattaa ttgctaagcg catacgatgc gagtaaacag tattatgaat tttgagtgtg
     4741 ccttcaacga tttgactttc tcccagactg aacgatattt cataagatat ttcttccatt
     4801 tcgggttttt cagataaacg atcttttaaa tctttaggaa gttgagcttt tccgtaaaca
     4861 actaatgttt cttgtaaaga atctttatat actttagctg tttctaattt tagagatagt
     4921 tctgtatctc cttctaatgg tgaataatca tacgtatcgc catcatttgc tgaatcgatc
     4981 aaatgaacga aatcaagata ttgtctatct ccaactatca aattcaattg cccatcttta
     5041 aaagaaagtt tatatttatt gttacttatc tcagttttgt ctatctcgat tgctgtttct
     5101 aatttttcat tatttttctc aaattcaaca acagtgtatc ctagtgctgg cacatttaca
     5161 tttattttta tgtcaagttc ataataagct ggttcatcaa aatattcgaa acctgtagca
     5221 atcatccgat aagcattttt ccgttctgga aaataaactt ctttttcaat gatattatag
     5281 ggactacctt tgaagtgtat tttctttgaa tcagctacta catgcacgat tttcgatcca
     5341 ctataattct cagtcgttgt gttgaaaaca aggacttcat tcgattttag ctttaatgca
     5401 tcagccatcc gttttacaat aaggttttcg attccgtcag ctatctcatt agcttctttg
     5461 attcgatgga aaatatcttc cgcaacatta tctgaaacac agccaccaat actatcatga
     5521 gcctggtttt gcaaaatctt tttccataac cgacgaagta ggcctttact gatatggata
     5581 ccatttcttt ctgcaatcag catgagcggc tcaactcgtt ttagaatttt ttgttccaat
     5641 ttaaaatttt ctattttcat ttttgttctt actgagccaa tagaacgatg aacacgagag
     5701 taaaccggct gacgaagttc acctttatat tcaggtaatt cttttttgtt tctgataact
     5761 tctacataag aaggataatc tgaaataata ttgtggaatt tactgtattt attcaacttc
     5821 tctacgactt gatcaaaatt taaaatcatt gacttttgat ctacacccga aggtactaaa
     5881 atatctttgt tttttccttg catatgaagc atttcaactg cttcatccaa cttttctttt
     5941 gcaaattcag gaatatcttt aattgcgtct aaggtcatca ttccagtcat ataaccaaat
     6001 ggaaaattca tggcataaac aaatttatct cccaatcctt tccagataaa ataaggagat
     6061 ggaaccatcg tttcaaagtt taatcctcgc caacacatga aattatcgat ttctacttga
     6121 tgcaaaagtg taggcaaatt cgcattgaac ccaaacgtgt caggcaaata ccctaccatc
     6181 attggatctc catatttaag gatcgtatca ctgattccaa tcattaagtt tcttaaaatt
     6241 gattcagcat ccactaataa ggcatctgtt tgtgcatacc acggtcccac gaatagttgt
     6301 cctgttttca tcagtcgttg gataacctta agcttttgcg gattgatctc caaatactca
     6361 tcgacgattg aagtttgtcc atctaaacaa aagtttactt tagggttcct ttccaattca
     6421 tctagaactt cagtaaagac ctgatcagac aatacgatgg catcttcatt tgtaaaatac
     6481 cattctctgt cccagtgcgt gtgttgaatt atatgtgcat ttaccatatc agattctcct
     6541 actttccttc ttctactgaa tttacttgtt gttctttttt atacttgatg gattttagtg
     6601 cattgaccat taacgctgta accactcccc caataacaat tgatactaaa tataaaggta
     6661 tattcgtaac aaaaggaatt gccataaagg ttgaaagcat tgtttcttga gtaacaccaa
     6721 aaaatccatt tagaccacat gctactgcag tgcccaccac gatgcttggt attactcgta
     6781 aaggatcttt cacggcaaac gggatcgtcc cttcagtaat ccccacaagg cccataatca
     6841 tagctgtctt tgcattatct cgttccgatt catcataaag ttttttgttt aaaattgttg
     6901 ctaaaaacat tccaaatggc ggaatagcaa ttcctaataa catggctgta ctaggtccaa
     6961 agcttccgct tgaaaaagaa gcaaccccaa aagcatatgc gattttattg ataggtcctc
     7021 ccatatcaaa cgccatcatt cctcctaaaa tagccgacat caaagcagca tttgctcctg
     7081 acatgccttc tagccaatta gtcagtcctg ttgtcattgc ttgaataggc gtagctagta
     7141 cataccataa tatgattcca acaactaatg tagtaacaaa aggaatcagc ataattgatt
     7201 ttagactaat aacttgcatc ggtactttta cttttttcaa tgcattagct aaatagccac
     7261 aagctaatcc agcaaccatt cctccaataa atcccatacc aagattttga cacatcatcc
     7321 cagtgataaa agctggtgct agaccgggac ggtcagcaat tgaatatgcg atataggctg
     7381 aaatcatcgg tacgataaac tgtaatcccg tctgaccaat cattcgcagg actccccaga
     7441 atgtatccgt ctggtcccaa acacctgtgc caccaaaaat gactgcaata gaagttagta
     7501 accctgctgc tacgacaagc gggatcatat aggaaacccc actttggaaa tgatttagaa
     7561 tttctcttcc ttgtttcttg aattccatta gtaactcccc cacgatatat tttttatttg
     7621 ttaattaatg caattgcttc atcaatcgtc tcttcaccat cttttactac ctctgaggtc
     7681 cctactgtta aaatcggttt accgtcaaag cgacctcgtt ctgaaacatt tgtatctact
     7741 gcaaatatga caacatctgc ctcggaaaca tccgaaattg aaagtttgtt ttctatcccc
     7801 atagccccct gtgtttctac tttaatatct aaccctttct tcttcgcaaa cttttctaaa
     7861 ttagcttttg ccatataagt atgggcaatc cctgcgatac atgctgttac tgctactatt
     7921 tttgtcatat gaaattcctc cttttaaaat gatagagctt ttaccaattc ttttttattt
     7981 ctcgaatctt ttattctttt aacaaactcg tcatccatca atcttccaga taattctgct
     8041 aaaatacgta aatgattagt acctttatca gtgtctttta ttgctaacaa aaagacgaga
     8101 tcaactggtt gatcatctaa tgcgccccat tcaacagacc gctttaactt ggctacagcc
     8161 actgttgatt caattactgc taggcttttg ccatgaggaa tggcgatatt tttcccaatt
     8221 cctgtggtca tatggttttc tctgtcaaat acgtcttttt gaaattgttt gtggtcagac
     8281 acaaacccat tagtctctaa caatttcgat aatttttcga ctacactttt tttatcagat
     8341 gcatttaaat ctatcactat attatttata ttgatttttt cactgatcat ttataagcct
     8401 tctttcaata tataaactaa tttttcaaac gacgaactcg tttttaaatt tgctaataac
     8461 tcatgattcg aagcgatttc attgaatgtt tcataaattt tattcaattc aggttttact
     8521 ttttcattaa gtgcaaaaaa gaaaacagaa tgaacaagtg tcccatccca atcaattcct
     8581 tctggattaa cgtagacaca gattactggc ttttttataa aatctacttc gatatgcggc
     8641 atagcaaaat cattcatcgc tgttgaagat agtttttctc ttttgattgc tccctctagt
     8701 atcccttctt tcgcgtaatt ttcttttacc aattttttgg taattatatt taaaacgttc
     8761 acttgattat cctttatttg gtgggtaaaa aagcaaaatc gtttatctaa taaatttaga
     8821 aaagcatctt ctgtttgttc atttaactgt agatactgtt ttacttttct gacatctgcc
     8881 cctaacatca aaggactaac aacaataaca ggaaaatttg tattttcaat cggaatcgtg
     8941 cttataacaa gttcatcagt gacttttcta tcttgtagtt cattgatgct tagtacatct
     9001 tctatctcaa ccatatctgc aaaaccgtta tttattcttt gctctaataa ttttgatgtt
     9061 ccatatccgc tactacaaac taatatcact ttcgttttgt caggtttgta acgatccaaa
     9121 agtgattgaa tatggagtgt tacgtaagcg atttcatctt cattaaaatg tatacagaat
     9181 ctctcttcga tttcctctaa taaatctaca cttatcataa atgacctttt aaaagaatat
     9241 ttgattttgt ccgtataagg gttatagata tttactccca atttgagtct tttaactgct
     9301 gcattgagat gtaatactag attattgatc aattcttcat caggataaaa ttcatttaat
     9361 ttaattctta ctaactcttg tagctcaaca cccatatcta aataggaaag cttcgtttga
     9421 tctaagtcgt gattcatagc atttttttca atcgaagtaa tgtgaatgtc aacataagct
     9481 tgttcatcta ttggcacaat aattgaaaat gctttttcaa tcattttgat taaaataagt
     9541 gtattctttg agatttcttc ttcagaatta ttactacgtg ataagaaatt gcttgtttca
     9601 atcctttcta tcgcaatagc aaggtggata gctaaagttt gaaattcata atctgtaaat
     9661 ttcaattgtg tcttattcat gtatcgatta agcaattcgg ctactttttt caatatgtcc
     9721 ttatttaaga aattattcgc ttcaccaatg aaattgatat tcaagtctaa gagatctgtt
     9781 ttactgttag atgctgtaaa gccaccccaa taatagttca caacttctga catcaacctt
     9841 cttttacttt tttcatcagc tttgatgcgc acgccatttc gatctgaaac taaggacatc
     9901 ccatgcttgt ttagtctttt tctaacatct ttcattgtgc tttctaccgt cgacctgctc
     9961 acgtataatt cgtcagcaag attttgaatc tttagagaat cattagtatt caataattta
    10021 gcaaacacat aagttagacg gtcctccgga gagtctgcga tattgatttt atgactggat
    10081 aataaatttc gaagtgtttc tttttttcca ataagcgaaa cgcctttttt aggttgaacc
    10141 aacatttgta catctggcat tttttggatt atttctttca aaattaaaat atctttgcta
    10201 attgttttcg tagatacata taattgttct gataattctt tgtaagttgt gtgctcattt
    10261 tcaatcaaaa agtatagtat tttcatttgc cgttcgctaa attcgatgcc cattatcttc
    10321 acctcctgaa tacagcttaa gtgtacaatt attatctcta ttttcataca gaagaaagtt
    10381 cttgattaat gtagaagttt ctaatattct tgattatttc aaatcaataa aaaagcctgc
    10441 aatatttata tcacaggctt ttttctctat taaatatata taagataata catttttcta
    10501 ctagttctca ctaatcttgt ataattaaac cctccgcaac ttgacggaga tggagaagat
    10561 gcccccgtct aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag
    10621 gtaaagcccg attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg
    10681 ctggaagact ttttcaacag aggtttgcgt tcctggtttg ataggattgg ccactgttaa
    10741 tcgaatgaat ggatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa
    10801 taaattctgt atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt
    10861 tgtatttatc gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata
    10921 aaaagagaaa aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc
    10981 tttttgcttc gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct
    11041 tgcaacagat tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg
    11101 ttccagtaat tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag
    11161 gccgacgatc gatagtctga tgttttcttc catttcttga aatgcttgtc ctttttgttc
    11221 attttcatcc aaccatgtat tcagtgcatc tactaggtcc attagattct tttctcgaaa
    11281 ttcgactgaa agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt
    11341 taatctcttg atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag
    11401 aagcaacagc catttaatca aatctaaacc ccactcccca caccgtatga atatacacag
    11461 catgtgcctt tgctagcttc cctcgaagat tggaaatatg catattcact gttttctcac
    11521 tatcaaaata tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt
    11581 tcggatgttc taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagcca
    11641 caccattcac atttacatcc ctcgactctt tgtccagtaa gatttccttg taatagatgc
    11701 gttgcgacag atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta
    11761 acaaaagttc ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca
    11821 aggaaacttt atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg
    11881 tctgtcggat ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa
    11941 ggatcaaatc gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat
    12001 caagaagttt gtatctctgt tcaagaacct cctccagtaa ggaatgaaca gcttcatcgt
    12061 cttcgataat caaaatagtt tccagcagga ccacctactt aatgtcagat ttttggaaaa
    12121 taaacattcc tagagctgat gataccacga ttgttactag actgaccaga atcattttcg
    12181 gtgcttgcga ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa
    12241 accactccgt tagaaattcc aagcctttga tttttgcgaa aacaccggga atgaaggccg
    12301 ttcccatgat ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg
    12361 tcaaagccac tggaacagat aatgtcccta atgtcagcag aagcaataca ggaaatactt
    12421 ttagaacata atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg
    12481 tgtaagcaag catggctagt cccgtgatca aaaccacgcc aagtgttgtg atcaaaagcg
    12541 taacgagaag tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc
    12601 gaatcgtacc gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca
    12661 gaagaatcca tgcagccatt ttttctacca ttaaaatccc tgttgcaccg ttgatgactg
    12721 tatccgttgc ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag
    12781 aataaaaaag cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc
    12841 atacttttat tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt
    12901 aagaaataat cttccagttt ttgcccaact aggtgaatac catccacagg aacttttgct
    12961 tctaccaatg cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca
    13021 cggctagatt gtacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt
    13081 tccgtcgccg aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc
    13141 gataattctt tgatcaagtg tccttcatgc aaaataccat agtgagtggc gatctgtgac
    13201 aattcatcta gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt
    13261 tggatgattt ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc
    13321 aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata
    13381 cctaaagaga aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc
    13441 aggtcttcga tttctctatc cgacacaccg ccgttcgctg cttggatacg taggttatct
    13501 cttgcagtta attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt
    13561 gctgcctgca aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa
    13621 cccataatag ttctcataaa ggtagactta cccgctccat tttccccaat caatccatag
    13681 atcattccac gtttgatttc gattgatact ttatctaatg ctttggcagc accgtatttt
    13741 ttggttatac cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt
    13801 tcttattgat gagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa
    13861 gaaagtgtaa attttccaat tgaatgtcaa aaaaaacaat cggacaatac gtgtctttcc
    13921 tattatcagg cagtttgtta tacgcttgta tcagtttcct gattctttct tttcgaccaa
    13981 agtcgtttct ctgtattcct cttcactgaa aatttttgct tctttcagat caagcttgag
    14041 taattttacc agaccggtta taaggataac tatagcaaga ctccctccca gaaaaattag
    14101 actgtctcgc agacgtggtt gcgttgtctt ccaatcaaag agcgcaccaa acagtgctaa
    14161 tccaagtgga ctggccacca tggtaatcgt tgttatcgtt gacataagtc gtccttgtat
    14221 atgcgttgga acttctcgct gaagccagac catataaaaa gcaaaagaaa acattaatcc
    14281 ttaaaaacgg acttatgaaa aataatacat gccgtttggg atttacaatt agctaccaaa
    14341 taagaggtta tgacaaaaga cttttcacaa tcccttcttc cgaataaacg gtgttctcct
    14401 gccaactcct cggtcacaag tcacaatatt tatcaaacat gagaagcaat ttttgtaaat
    14461 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcagaagcgg ataaataata
    14521 ttgtttgatg atactttatc tttttcccgt ttgtttatag atcgaatgat attatagacc
    14581 aagaatccta cctattaaca tagctaaaaa cacaaaaaag gcactttcca aattgtggaa
    14641 agtgccagaa aagttaggat taaggtaact gcgctcagca ttgcttcgcc cagtacgatt
    14701 ctcgtaactc gtgaactcga acgataatcg caattaacgt tttgagaact gtgaagcttt
    14761 acgggctttc ttaagacctg gttttttctt cgtatcattg aataagtttg aaaaaccttg
    14821 atttataaag gttttcccgt aaatatattt tattatattt gattaaattt gaaatgtttg
    14881 tgttctaaaa tgtgttctaa tttaaaggca gtatatttcg tattttagat taaattttta
    14941 ggctcattca ttctcaaatc cattctattc agtcaataaa aattttaaaa agactttata
    15001 aacaaagatg ttcaagcgag catcttattt ttttgtgctt aatttgaaag gagaacattt
    15061 catgtataaa aaacaaaaaa agaaattatg tgtattaact attttgagtt tggctgttca
    15121 agcaattgtt ggagcaattg ctcctacgat tacttttgct gatgaaatta cccatccaca
    15181 aacggtaacc gtagaactag atttagcaca tcaatatgct gttgaaggaa cttttagtga
    15241 tggtcgtcca atgtcagaag tcactgttcc gcattatgca gtgtacaacg gctttaaaca
    15301 agatattttc tgtattgaac caggagtccc gatttacaat gagttcactc ctggctatga
    15361 gaaaaacccg ttaccagata tgtccgataa agccaaatta gtttccgttt tatggaaaaa
    15421 agtaggtaca gatgcagata ctcatattgt ggcacagaag atgatctggc aagaagttaa
    15481 tgggtatacc cttcactcaa taaaacgatc agatggtagt gccgtaaata tcgcagccat
    15541 tgaggcaaaa ataaatcaag ccattgacga ttatcagaag aaaccaacct tccataatag
    15601 tacggctaaa acagtattag ggcaatcgac tactgtgacg gatacgaata atctgaattt
    15661 gtcagagttc gatgaggttg tggaaaatgc cgcaaacatt gattatcgtg tgaatggtaa
    15721 tcagttgata atcaccccaa atgccaattc aaaagaaagt ggtgtgctga cgcttaagaa
    15781 atctgctggt accggaacac ctgtcgccta taaaaaggct ggccaacaga ctttaatggc
    15841 cggggcgatt gataaaccta atacctacac aatcaaaatt gatgtggaaa cggagggttc
    15901 tttaaagatc aaaaaagtcg ataaagaatc gggtgatgtt gtaccaggaa cggttttcca
    15961 tttagacttt ggaaaaactt tacctgcaaa agacgtgact actgacaaag aaggcattgc
    16021 tacattggat gagattcctc atggtacaaa agtaaccatt accgaaaaat cagtaccggc
    16081 gccttatacg attgatacca cccccatgac taccaccatt aaagcaggcg aaaccattta
    16141 cgtgacttca aaaaacgcac gggaaaaagg gcaaatcatt ctggataaaa gcggagttga
    16201 aacagggagt gatctttgga atgacaatta ttccttagct ggtaatacgt ttgccattcg
    16261 caaagacagt ccaaccggtg aaatcgttca agaaatgacc acggatgaaa acggtcatgc
    16321 ggaaacacca aaagagattg ctaatgcgtt ggaattggga acctattatg tgaccgaaac
    16381 taaagctagt catgggttcg tgaatacgtt caaacctgta aaagtcgaat tgaagtatgc
    16441 caatcaaacc gtagcccctg ttaccagtaa cgtaaaaggg caaaatcaag aagtcactgg
    16501 cgaaaccact ttgacgaaag aagacaaaga tactggggat aaagcacaag gtaaggcagt
    16561 ctttgagggt gctgaataca ccttgtttac tgctaaggat ggaaaagctg ttaaatggag
    16621 tgaggctttt aaaccagagt tagcgaaggg aacgaaaggt tctgatgaaa cggtcacttt
    16681 gactttagac gaaaagaacc aagttgccgt taaacattta gctatcaacg agtattattg
    16741 gcaagaaaca aaagcgcccg aaggatatag ccttgatgaa acaaagtatc ctgtatccat
    16801 caaaaaggtg gatgatagcg aaaaaaatgc cgtgattacc cgaaatgtta cggcaaaaga
    16861 acaagtgatt cgctttggct ttgatttctt taaatttgct ggatcagctg cgggcactac
    16921 cgaaactggc ttcaatgatc tgacctttaa agtgtcgcca ttggaaggga ccaatgaaat
    16981 cacaggtgcc gaagatgaag cgaccacagc ttataacgag caattagggt ttgatggtta
    17041 tggcaagttt gaaaatcttc cttatgggga ttatttactt gaagaagtag aagccccaga
    17101 aggttttcaa aagattacgc cattagaaat ccgttctact tttaaggaaa acaaagaaga
    17161 ctttgtgaag agtgagtatg tctttaccat taccgaacaa gaccaaaaac aaccaattaa
    17221 aacggtgacc gttccttacg agaagctgac gaacaaagca ttttctgtta gtttgaaccg
    17281 tttaatgctc tatgatttgc ctgaggaaga agatagttta acttctcttg cgacttggaa
    17341 agacggaaac aaagaattaa cttctctgga ttctaccgag ctagttgata aattgagtta
    17401 caatctacat gaaatcaaag aagactggta tgtggtagcg caagccattg atgtggatgc
    17461 gacaaaagct gcccaagaaa aagatgaaaa agccaaacca gtggtgattg cagaaacaag
    17521 cgcaacgttg gcgaacaaag agaaaacagg aacttggaaa attcagcata aattaaccgc
    17581 cgaacaagtt ttaaataaaa ctattgtctt attcaactat gtgtatgaaa ataaggaagc
    17641 ttttgaagca ggcgataagc cagtagcgaa ggatgttagc ttgaataacc aagcccaaac
    17701 tgtcagttgt acagtggaac accatgtttc tatccaaaca aaggcccacc tagaaaacgg
    17761 ttctcagacc tttactcatg gtgatgtggt ggatatgttt gatgatgtgt cgattactca
    17821 tgatgtactg gatggctcaa aagaagcttt cgaaacaatt ctgtatgctt tattaccgga
    17881 tggtacaaac aaagaaattt ggaaatctgg caagattgat tatgaagtaa atgataaaga
    17941 attcaccaaa actgtgcttg cgaaaaaagt agataccgga aaatatccag aaggaaccaa
    18001 gtttactttt gcggaaatca attacgataa agatgggaat atcaatggaa aacacaatga
    18061 agatttgaaa gaaaaatctc aaaccttaac accaaaagaa gttccaacca tactaagtac
    18121 gccgaaacaa ccggaaacac caactgttcc aagtggctct caagaatcta gtcccacagt
    18181 gaagaccttc cctcaaaccg gggagaaaaa ttccaatgtt ttactgttca ttggttttac
    18241 tttgattttt gcgacggcgg gctattattt ctggaatcgc cggaactaag gtgatgagat
    18301 gatacaagta aatcctaaga accaacgtgc gcgaccgcct cctaaaaaag tagtcgcgct
    18361 atttttgtat ccaaaactaa caatgaaaag aggaaaagaa gatggaattg aaatttgtcg
    18421 tgccagatat ggctgaaacc tttggaaagt taagttatgc cggtgaagga gaaattataa
    18481 cagaaggata tggacggaat actacggtga ttggtcgcag ttatcatttg tattccagta
    18541 agcaacgggc cgatgatatt gaggtggtag tagctgcgga agccggtgaa aaggattttg
    18601 atcaagacca accgctaaaa gccgtgaatc cccatttggt tgccaaaggc tatgagattg
    18661 agaatcgtgg gtttactgat tacgtgttgt atgttgatga tttagtgaaa gcataggagg
    18721 aaaagaagat gagattaaca gaaggtattg tcgtagattc aggtttaaca tttgggaagt
    18781 tgcgtttttc cgcattacgc cgagaagtac ggaagcaaaa tgaggatgga acaattggta
    18841 atgaggtaaa agaacgaacc tataatttaa aatcctctgc acaagggcga atgattcaag
    18901 ttagtatccc agctaatgta cccttacgcg agtttgctta tgatgcagag gtagagttgg
    18961 tcaatccaat cgtggatacc gttgccaatt atgtttttcg agaaggaact accgttaatt
    19021 ggttcattaa ggcggatgat ttagtcttga agcggcagcc gaatcaagga aatcctacga
    19081 accaaaatga aagaaagaaa taggtgaaga ttatggaaat taatgttgaa catattttag
    19141 attgtctcga tcaatatgga aaaggcgagc tgactgagga acaactcgtt aaagcattaa
    19201 cctctgatga aaaaatgttt ctgattatac atcaaggttt acttggagaa aatagcgaca
    19261 gtgaagaaga ctctgatgta ctagactggt taggagaaga agagtctttc tttatagttg
    19321 tcgaagtaaa tgaaaactta tgtcggcagg cagaatccat tttggaagaa attggtgtgg
    19381 agatgccgga tgctattgaa ggctttttaa atcaattggt tgaaacgaaa caactcccct
    19441 cctcgtagca gtaaatgact agcataactt tttgaaattg cctaatgaaa tcctattggg
    19501 ctttttcgat tggaggtgga aaccatcaaa atgtacaaag gacatcgcat acgcgcagga
    19561 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
    19621 atgagcgtct tttattttca agagtttaga caatttgata tttctaagct atcactttct
    19681 actattgaaa tcgtttggtc tataaaagat ttagtatgtc tgcttggaag tctcgctttt
    19741 tcaggagcta tgatattgct ttatattcac ttttttcttg atcattggcg aagcttgtgg
    19801 catcgacaaa agctagctcg gatgattcta gagaaccatt ggtatgaagt gaaacaaact
    19861 caaagtgaag gctttttcaa agatttgaat agtagccgaa ccagagagac tatcagttac
    19921 ttccccaaaa tctgttatcg aatgaaagat ggattactgt ctattcgcgt tcaaatttca
    19981 cttgggaaat accaagatca gcttttaaaa ttggaaaaga agttagaaag cggactgtat
    20041 tgtgagttag tagaaaagga actcaaagac tcctatgtgg agtatacttt actgtatgat
    20101 atgatcgcca atcgaattgg gatagacgaa gtagtggcag aaaatgggac tctgcgattg
    20161 atgaaaaatc aagtatgggc ttatgattct ttaccccata tgttaatcgc tggtggcacg
    20221 ggtggaggga agacctattt cctcctcacc attatcgaag cactattgaa gtcagatgca
    20281 gaattgttta tcctcgatcc caaaaatgca gatttagctg atttaggtac ggtgatgcct
    20341 catgtttatt cacaaaagga agaaatttct gcttgtgtgg aagatttcta tgaacgtatg
    20401 atggctcgta gcaaggcaat gaaagaaatg tctaactaca aaacaggaga gaattatgcc
    20461 tatcttggac ttccacccaa ctttttaatc tttgatgaat acgtggctta tatggagatg
    20521 ttaacgacga aagaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttgggt
    20581 cgtcaatctg gtttctttct gattttggct tgccaaaggc cggatgcgaa atatttagga
    20641 gacggaatcc gcgatcaatt caactttcgc gtggctttgg gtcgtatgag tgaactcgga
    20701 tattccatga tgtttgggga ggtcgaaaaa aacttcttta tgaagcacat caaaggtcga
    20761 ggttatgtgg atacaggtgg cagtgtgatt agtgagtttt atacgccact tgtaccaaaa
    20821 gggtatgatt tcttagattc aattagtaaa ttagaaaaga tgaatgattg agtgatcttc
    20881 aatactgact ttatacttca agtaacgtat aatactttta tatagtagga agaaagggga
    20941 taattaatat ggaaagtaat gattcttatt tagagtatgc aaaagcaaat aaggataagt
    21001 ttatttctta tattatcaaa gggaaatccc ccgaagttgg acgagacgca gtttttatgg
    21061 cagggagtcc tggggctgga aaatcagagg ttgccattgg attagctgag aattatgata
    21121 accatgtgat tattgatgcg gattactttc gcacccagtt tcctgattat aatggaaaaa
    21181 actctagtct ctttcaaaaa gcatcttctt ggttggtaga gcaggcattt agatatgtct
    21241 tagatcatgg ctattctttt attctggatg caacttttgc aatattaagt gctgaaaaaa
    21301 atatctcgcg ggcgttgaaa aaacattttc gagtgacgat tttctatgtt tatcaagacc
    21361 ctaaaatcgc ttggaaattt acacaagaac gtgaaattgc agagggacgt catgtgccga
    21421 aaaaggcatt tattaacgct tttttcaaat caagagagaa cattgcaaaa gtaaagagta
    21481 aacatccaga tgtattgctt catatcataa taaaagatta tacgaataat atttccgaag
    21541 ttcattatgc tacggataat ctagaattat ttttaccact agggtatagt aggaaagatt
    21601 tggaggttca attacatgac tgagaaagaa caagtaacga aaattgtaaa gaaatacaat
    21661 aagagcattg cggatttatc ggaaaatgct actgcaaaag agttcaaaac ggtgattaaa
    21721 tatgtagccg atcaggccaa tgaaaaacaa cggaaattag ttggattaaa taaaaaatag
    21781 taggacaaat taataatgta tgagccaaga gcaaaatgtt cttggctttt ttctttgcaa
    21841 tgaatgaggt aatgtatgag atttttagat ttattcgccg gtattggtgg ctttcgttta
    21901 ggtatggaac aggcgagcca tcattgtatc ggtttttgtg aaattgataa atttgctaga
    21961 cgtagttata aagcaattca tgatacaagt aaggaggtgg aaatgcatga catcacaagt
    22021 gtatcagatg agtttattca atccctcgga ccagtggaca tcctttgcgg cggatttccg
    22081 tgccaagctt tttcaattgc gggaaagcgg caaagatttt ccgatactcg aggtacttta
    22141 ttctttgaaa tcgctcggtt caccactctt ctccgaccta agcttttatt ccttgagaac
    22201 gtccgaggat tgctcaatca cgaaggaggg gctacgttcg agacgatcct ccgaacgctg
    22261 gatggattgg ggtatgacgt ggagtggtag gtgcttaact caaaggccta cattccccaa
    22321 agccgggagc ggatattcct tatcggacat tctcgagacg catgtaccga acaagtattt
    22381 cctatcattg gaccgtcttc aacatctgat caaaacatca gaaatttggt aaatatcaat
    22441 ccttccaatc gaggaatggg tggacaagtc tatggttcag atggcgtagc gccaacctta
    22501 acaggtgatg aaggaatcaa aatcgcactg ccagcaaggg atggaatctc tgtagcagga
    22561 ctattaccgg gaaagtttga acaaggtaat cgagtttatg aagttactgg aaccgccccc
    22621 accttgtcaa cgaaacaagg tggtacaaaa atcatgattc gaaaaaatga aacagaatat
    22681 caagaggtga agccagaaga cagtgtgaat ctggcttttc ctaattccac aagtcgccga
    22741 ggtcgtctgg gaaaacagag tgttcatacc ttactaacag gagaccaaca agcgatcgtc
    22801 acggatcagt atcaaattcg gaagctaaca cctagagagt gttggcgact tcagggcttt
    22861 cccgattggg cttttgatcg agattctcaa gtcaattcag atagtcagtt gtataagcaa
    22921 gccggaaact ctgtaacagt tccggtcatt tttgatattg ccagaagatt aaaagagttg
    22981 gatgtacatg gtttatgatg agttgattgg agaaatttat tgggtgattg gaaagattca
    23041 gtcagaccca gaattagaag aacaattgcg acggttaaaa tttgatattc gcaagaatgg
    23101 tgtcaaagtc gctggcgatc catatttaat gaatgaggag acggatgctc gaattgaaat
    23161 aaatcaagta attgcagaat ttgagcggat tgcggaccaa gcaaaagaac cagatattcg
    23221 tcaatacttg ttagaaatga aagcagagtt ggaagtaaat ggtattacag atgagtaacc
    23281 taaaccaaca caactgactg ttaggcgctg accgcggagg attcgcgaag cagaaatcct
    23341 ccgtggtgct gtgcctgttc atggacggcg ttagccgacc atgatgtctc acccccgtat
    23401 ctaacagggg ggtacaaaaa cacaaaaaat aagtaagcaa ccattaagaa tgcttgttgt
    23461 atcaaagata gtaagtaatt tgtataggtg ttaactcatt tgaaaaagtt aacaccttga
    23521 atttggaggt gagaacttgg cacaacggaa tttagattat cggctattaa aagaccgacg
    23581 caacgaatac ggcgtttcac aaaataaatt ggctacggct tgtggactta gtcggccgta
    23641 cttaaatcag atagaaaatg gtggcgtaac cgcatctata aaaaccatga ggaaaatttt
    23701 tgaccaactg gaaagcttta atcccgattt gcctttgacg ttactgtttg actatgtaag
    23761 gattcgcttt cccacaacgg atgcacgaaa aattattcaa gaaattctcc atttgaaatt
    23821 tgagtatatg ctccatgaag attatgcctt ttactcctat caagaacagt atgtcatggg
    23881 tgatattgtg gtgatgttgt ctcatgaaga agataaaggt gtgcttttag aattaaaggg
    23941 gcgaggttgc cgacaatttg aaactttttt actcgctcaa aagcgtagct ggtacgactt
    24001 tttcgaggat tgtctaaagg ccggtggcgt gatgaaacgc ttggacttgg caatcaatga
    24061 tcgagtgggg ctattggata tacctgagct aacgaaaaaa tgtcagaagg aagaatgtat
    24121 ctccttgttt cgtaccttta aaagttatcg ttccggggaa cttttaaagg ctgatgaaaa
    24181 ggacggcatg ggcaatacct tatatatcgg cagtctcaag agtgaggttt atttctgttt
    24241 gtatgaaaaa gactatgaac aatatatcaa gttagggatt ccattagacc aaactgagac
    24301 gaaaaatcgt tttgagattc gactgaaaaa tgaccgtgct tttcatgcga ttcaagattt
    24361 gttaaaaggt cgtagcattg agagcaccac gttttccatt atcaatcgtt acttgcgatt
    24421 tgcggataaa gtagaaggta aaaggcgaac caactggccc ttgaatgaac aatgggggcg
    24481 ttttattgga cggaatcgga aagagattca attaacttcc gagccaaaac cgtataccat
    24541 tgaacgaacc ttgaattggc taggccgaca agtcgcccct acgtggaaaa tggctaagga
    24601 gttggatcga ttgaatcgga caacctatat ccaggatatg gtacagaatg cacgcttatc
    24661 agaccgacat aaaaagattt tggagcaaca aagtatggca attgaaaatt tgattatatg
    24721 aaaagaggaa tcgtcatggg aattgtaaaa gatatttttt tggtatttgg tggcagtatg
    24781 cttggtgtta ctgtgatgtg cttgatgaat gcaagtgcag cagcggatcg agcaatggaa
    24841 aagaaaggaa agtgacaaga tgaatttcgg acaaaattt
//
LOCUS       DACECJ010000035        24520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.035, whole genome shotgun sequence.
ACCESSION   DACECJ010000035 DACECJ010000000
VERSION     DACECJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.035"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWO09_001478"
     CDS             949..1422
                     /locus_tag="IWO09_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0264072.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWO09_001479"
     CDS             1431..1658
                     /locus_tag="IWO09_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264073.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IWO09_001480"
     CDS             2294..2665
                     /locus_tag="IWO09_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264074.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IWO09_001481"
     CDS             complement(2921..3163)
                     /locus_tag="IWO09_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0264075.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IWO09_001482"
     CDS             complement(3195..4097)
                     /locus_tag="IWO09_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0264076.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IWO09_001483"
     CDS             complement(4110..4298)
                     /locus_tag="IWO09_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0264077.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWO09_001484"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWO09_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0264078.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IWO09_001485"
     CDS             complement(4913..5806)
                     /locus_tag="IWO09_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0264079.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6821)
                     /locus_tag="IWO09_001486"
                     /pseudo
     CDS             complement(5884..6821)
                     /locus_tag="IWO09_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(6855..7205)
                     /locus_tag="IWO09_001487"
     CDS             complement(6855..7205)
                     /locus_tag="IWO09_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0264080.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="IWO09_001488"
     CDS             complement(7238..8140)
                     /locus_tag="IWO09_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0264081.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="IWO09_001489"
     CDS             complement(8133..8990)
                     /locus_tag="IWO09_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0264082.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9324..10133
                     /locus_tag="IWO09_001490"
     CDS             9324..10133
                     /locus_tag="IWO09_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0264083.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10173..10670
                     /locus_tag="IWO09_001491"
     CDS             10173..10670
                     /locus_tag="IWO09_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0264084.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11055..11128)
                     /locus_tag="IWO09_001492"
     tRNA            complement(11055..11128)
                     /locus_tag="IWO09_001492"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Ile,seq:cat)
     gene            11315..11638
                     /locus_tag="IWO09_001493"
     CDS             11315..11638
                     /locus_tag="IWO09_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264085.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11802..12056
                     /locus_tag="IWO09_001494"
     CDS             11802..12056
                     /locus_tag="IWO09_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264086.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12126..12371
                     /locus_tag="IWO09_001495"
     CDS             12126..12371
                     /locus_tag="IWO09_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264087.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12447..12812
                     /locus_tag="IWO09_001496"
     CDS             12447..12812
                     /locus_tag="IWO09_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264088.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12873..13436)
                     /locus_tag="IWO09_001497"
     CDS             complement(12873..13436)
                     /locus_tag="IWO09_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264089.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14004..14204)
                     /locus_tag="IWO09_001498"
     CDS             complement(14004..14204)
                     /locus_tag="IWO09_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0264090.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(14956..15669)
                     /locus_tag="IWO09_001499"
     CDS             complement(14956..15669)
                     /locus_tag="IWO09_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0264091.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15662..16747)
                     /gene="uxuA"
                     /locus_tag="IWO09_001500"
     CDS             complement(15662..16747)
                     /gene="uxuA"
                     /locus_tag="IWO09_001500"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0264092.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16764..17207)
                     /locus_tag="IWO09_001501"
     CDS             complement(16764..17207)
                     /locus_tag="IWO09_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0264093.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17241..17594)
                     /locus_tag="IWO09_001502"
     CDS             complement(17241..17594)
                     /locus_tag="IWO09_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264094.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17706..18107)
                     /locus_tag="IWO09_001503"
     CDS             complement(17706..18107)
                     /locus_tag="IWO09_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264095.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18144..18653)
                     /locus_tag="IWO09_001504"
     CDS             complement(18144..18653)
                     /locus_tag="IWO09_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0264096.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18675..19532)
                     /locus_tag="IWO09_001505"
     CDS             complement(18675..19532)
                     /locus_tag="IWO09_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0264097.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19550..20383)
                     /locus_tag="IWO09_001506"
     CDS             complement(19550..20383)
                     /locus_tag="IWO09_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0264098.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20397..21194)
                     /locus_tag="IWO09_001507"
     CDS             complement(20397..21194)
                     /locus_tag="IWO09_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0264099.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21227..21511)
                     /locus_tag="IWO09_001508"
     CDS             complement(21227..21511)
                     /locus_tag="IWO09_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0264100.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21508..22509)
                     /locus_tag="IWO09_001509"
     CDS             complement(21508..22509)
                     /locus_tag="IWO09_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0264101.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(22511..23413)
                     /gene="gnd"
                     /locus_tag="IWO09_001510"
     CDS             complement(22511..23413)
                     /gene="gnd"
                     /locus_tag="IWO09_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0264102.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            23577..24425
                     /locus_tag="IWO09_001511"
     CDS             23577..24425
                     /locus_tag="IWO09_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0264103.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         7168 a         4881 c         3969 g         8502 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt tttttcgata cgattatctg actgatctag attaatcact
     6601 accaaagaaa atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct
     6661 tccggatatt cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc
     6721 tcaatctctt cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccacctttt tcttaagaat gttttttgaa aaaattcata atgacaaagc
     9241 aagttagttt cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg
     9301 taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc
     9361 ttgatcgaaa tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa
     9421 atggcggaat cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt
     9481 ccgaattagg aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga
     9541 aggatggaaa aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa
     9601 aagccttctt ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag
     9661 atgatcagct gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt
     9721 tcaatttaca gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta
     9781 gctatcttaa attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag
     9841 atctgcaagc agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc
     9901 ctacaggtat cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat
     9961 cgagtgacat catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg
    10021 ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa
    10081 cactgacgaa tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat
    10141 aaaatgacga tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc
    10201 atattcttga tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta
    10261 ttgctcaaag attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata
    10321 taccagttga actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc
    10381 tttctccaca agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa
    10441 ctttagctga aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag
    10501 tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt
    10561 ttcctgaagc agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat
    10621 ccgctttaga gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat
    10681 cttcatttac gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg
    10741 tgctttttct atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac
    10801 gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca
    10861 aaatatgagg aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc
    10921 acaacctctt ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag
    10981 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    11041 ttcacaacct cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt
    11101 ctgctctaac caactgagct aaaggtacat cataaataaa atttattttt gttacttttc
    11161 aactatagca cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt
    11221 attcttattt cttttacttg tgtcataact attttgttac tttaattttt atttgcttat
    11281 tttagaaaaa atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt
    11341 tattgcgatt ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca
    11401 aacctcagga attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct
    11461 tgtcatgatg ctcagctata aaggaaaaag atatgcccgt attcaaacaa aagtatttgt
    11521 taaatatgtt tccattcgct atgggatcat cattctttcc tttttagtga atgcgatcgt
    11581 cctcttttta tatgtattga attacttaga tatcaatcca ttgatttttt ctcgatagat
    11641 aagacacatt cagcggaaca gttcctgctt atcagcagaa aactgttctg ctttttcatt
    11701 tttcattgat ctattcttct tttattttcc tagcatttca tttgtgatgt atgcaataac
    11761 atgttatctt ttattatatg aatcgttgag gagtccgtta catgattacg ttggtgagag
    11821 ttttgttttg ggtgcctgcg gtcgcactgg tcgcaagtat tgtttatctt atgaattgga
    11881 acaaagaacg gttttattta gctattttga ctcttccggc tatctacttt atgtggaaag
    11941 tttttaatta caactacttt gaacccgata gtgtttttat agaagaatta tcaggactgg
    12001 ttttgtcttt attgatcgtc attctttatt tgatacgttt aaataaaaaa cattgagtac
    12061 tattgattgc atgtgtagct ttctatagtc attataacta ttctactata atgaaaggtg
    12121 attaaatgag tatcgaagaa gctattttac tggatacttt acgttgtcgt ctgaatttct
    12181 taagaactgg ccgatcttta tctcaagtag ctgtttctag cgcgttaagt attcctttat
    12241 tcaaatatcg taagtacgaa gaaggagaag aaattccaag catcattgaa ttaatctata
    12301 tcgctgactt ttacgatatt tccgttgatt acctaattgg tcgctcagaa acagcaagca
    12361 aactgaagtg attttttcca gccttggtgc tggttttttc atttaacgtc taaggttaac
    12421 aatctatata taaaggagac atttctatgc gattatggca tgaaaaattg atttcaaagc
    12481 ttcctcgtca acagctttta ggacaacatc gggaatgctg tgcattaaga ggaaatggct
    12541 ggggaaagaa acatgctact gtggattatg tttttcacta ttctccttat agattattcc
    12601 aatatcatca gcttgtcatg gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt
    12661 ggaaaaatcc tatgtatcgt ggcaaatcat gcgaagcatt ttttgattta cctactgtag
    12721 agcgcaacgt gccgatttat ccggaacacg atgaagttta tctagcagaa tgtttagcaa
    12781 atttggagca aaaaggaatt tatcttacct aatataacta gaaaagcctt ctatctcctc
    12841 ttacttcatt aaaaagcttt aaccatctta gatcaagcta aaatgtcgta agttttaggg
    12901 ttatcttcat cctcacgtgc aaaactaaaa gcgtactcta gttttggttc atcgccaact
    12961 tccctagaca taaatgaaca tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct
    13021 gtatacgatg cacagcacag attgcttctt ttgatctttc ttttgatttt tctgttgctt
    13081 cttctacacc ttcttcgatt actacatcga tgcttacaat tgcttctgcc taatattccc
    13141 atgcatcgtc gatttaagcc tctgtagtca ctgtgatcgt ttccacattt taactcctct
    13201 gtcgtggctt cagttgattt tgtgatcagt tcagttgcta agccagagtc agaacaacct
    13261 gccattgcta gcgttaatat tgatagtaaa aatagttttt ttatcataag gttccccccc
    13321 tgaaaataga ctaaaataat aacgctttct ttttttgttt tcagacgaaa attaataatt
    13381 tctaaatatt ctttaaaaaa gcttataact attatgcctt ttttaatata taacacagtt
    13441 atttttaaaa ataagaatgc taaatcactc tacaataacg gcgaaagcac aaaaaataaa
    13501 aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga tctcattctt
    13561 tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt atatattcgt
    13621 attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg tccgttactt
    13681 aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt ttcattaatt
    13741 gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc aaactcattc
    13801 attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac atcactttat
    13861 tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat acactactat
    13921 tcagcccatt caacgaccaa tgaatttttt cgtacaaaaa aaccgtcttt tctttcgaaa
    13981 agacggttaa aagtcaattt ttattaagct ttaacaatgt ttgttgcttg agggccacgt
    14041 tgaccatctt caacatcgta agacactgct tggccttctt ctaaagtttt gaagccgtca
    14101 ccttggatag ctgaaaaatg tgcaaataca tcatttccat cttcaccagt gataaagcca
    14161 aaacctttgt ctgcgttgaa ccattttact gtaccgttat tcataaataa attcctcctg
    14221 atacgtatca atgtacgttt tttcgttgca ataaatattt gataggcaaa aggagtttta
    14281 tctatgtaga tatatcgctc aaattacgtt tcaaaataga ttactcattt aatatatcat
    14341 gctttttcta ataacacaag gataatgcaa tgaaatataa aattcattgt cttctattgt
    14401 cttcgaatcg aagaaaaagt tgaataaccg cgattgattc actttttgtt ttcttgttta
    14461 tttgctttta tctgcaactt ttgactttaa aatttcttaa ctatgaaaac ctttcaattg
    14521 gtggtatact tataaatgga atttcgttat caacgcgttc gttgcctttc agcaagtatt
    14581 taaagaaagg agaatggcta tgacattcta taacaaagat atacaatatc gagtaacctt
    14641 ggatacagat ttgaatctat ttgtcgtttt tgacaaagaa aataccaatc atgtagcgac
    14701 aggtgttacg atcgaacaag cagtgcaaga attaaaaaaa tctgcataag gagtggaaac
    14761 cctcatatgg acgattctcg ttcttctctt taaacgcatc tactggtaga taacagtaga
    14821 tgcgttttgt gtttgttcta aattgttcac tacaaataag gtagatactt caaaataaaa
    14881 aattacttct ttttcatctg ttccctcagt cgtgcaggac aataaataaa gaggactaga
    14941 aaatagtcct ctttattaga gatacaaccc tttactttct aaaaatcgtt ttgcttgatg
    15001 caggtcataa aattcatagt ctgctttatt ggattcatat aaacttacat ctactttcaa
    15061 cttatgcttc ccattatgat cgggcaaccc tatcaaatga tctttgatga gaaaagacaa
    15121 aatgcccgct cgccttgctg cttcgatccc tgcaatcgag tcttcaaata caagtgcttc
    15181 acttggagga atattcattt ttgaacaagc tttcaaaaag atctctggtt ccggctttcc
    15241 tttttttaca tcatcccctg aaatgatttg atcaaacaag gcatatagat tttccatttt
    15301 taaatacatt tctactttta cccgtgtatt agacgaagcc acgattgtat ggattcctct
    15361 ttcttttgca aaagataaaa tttcttctgc tccttttttc gtatatactc tcttatcttc
    15421 tttcacgatt ttttctttat attgattgat tgcttctcgc catttgatat atggaacatc
    15481 ttctccgtac aaaatgccca tttgttctct tatcgccatt tctgtttggc cagttagtaa
    15541 ataatagact tccttacgca tttcataatc atacttttgt gctgatttta cataagcacg
    15601 ataagctaaa cgtccagttt ctaataaaag gccatccata tcaaagatga agagcttgat
    15661 tttattcata atgctctccc agtggttttt ccttacttat cgcctcatat agaccttgca
    15721 tgtaagtgag tcccattgca cgatcataca ggccatatcc aggcatagcc acttcacccc
    15781 acacggttct accatgatca gggcgtatag gaccgtcgaa cccttcttgc accaacgctt
    15841 tcataatatg atacatatca agggagcctt ctgtagaggg atgtgccgtt tctttgaact
    15901 ttctttctcc taaaaactcc acgtttctga agtggacaaa atggatgcgc gaacctacct
    15961 catggatgat ttcaactaaa tcgttttccg gatttgcccc taaagatcct gtgcacagcg
    16021 ttagtccatt atatggagaa tctactgcat ttagaatttt cttcaagtcg ccaagatttt
    16081 tcgtcactct aggaaaacca aaaagttccc atggcggatc atctggatgg atagccattt
    16141 ttacatctgc tttttcgcac actggaatca ctttttctaa aaaataagtc agattttcaa
    16201 ataattcttc ttcggtcatt ccttcataca gtttttccag ctgagcgaat tttttcaacc
    16261 gttcttcttc ccaacccggc aatttgaatc cctttgactg gctgtgtatc aattgataca
    16321 tttctcctgg ctccatctga tcgacgattt tctgatcgta taaaagagaa tagcttccat
    16381 cgttgttttc attaaacaaa tcagttttcg cccatccaaa aattggtttg aagctataac
    16441 aaatcaatcg aaccccacat cgagacaagt ttttgattgt tcgtatatag ttatcaatat
    16501 agtaatctcg ttcatctgtc cctgccttga ttgcatcgtg gatcgctaca ctttcaattc
    16561 caagcagttc cagtccttgg ctctcaacag atgctttcaa ctcctcaatt tctgaaatct
    16621 cccaaacatc tccaggcatt ttagtcagca aagttccaat tatcccattt attcccggta
    16681 tttggcgaac atattttaat gggatcgaat cccctttttc accataccat ctaaatcccc
    16741 acttcatctt ttcagccttc cttctaaaaa tcgtcctcat ctaatttttt ttcttccgct
    16801 gaagcatgcc aaacagttaa attatctttt ccttgtttga caatttgttc caccgcttct
    16861 tctatcggtg tgtgaagcat ctgatgattg attgcttcta acaacattgg cagattcacg
    16921 ccccctatga cataagtggc tgctttcttt tcttcagtca gttcattgac tgccaagagc
    16981 gcttgattgt acggacttgc gctaattaga tcaacaaata ctagcatggg ttctgctgtg
    17041 ttgatcatag ttaatttttc tttgaccttc tctccgaatg tttggatgtc ctccccagac
    17101 tctaagcaca ttgtttcaat atggctcgat gtcccgacaa ttacttccgc cgcatctttc
    17161 agtccttcgc tcaacttacc atgtgtaacg atcatgattt ccaacattag tcttctcact
    17221 ccttgttttg cactttttac ttatcgaaaa agagcaataa tctgattggc attttgccaa
    17281 ttaaatgttt tccctcgtaa atctgttttg gtcaaataga agaactgcgc ttgctttttt
    17341 cttgcactaa aaatataatt gacgtttttt tctttcaact tatcaacttg tttcagccac
    17401 tgttctttat ttgaaaaata atttcccgaa aactgtagcg gtgtatctgc cccaacagag
    17461 tctagataaa aaagaatagc ctcttttttc atacccgatc tcacgctgtc taagcccttt
    17521 tctccataac agccttcatc aacaaacgca tacgctacac tcggatcatc aatcgtacgg
    17581 atcatttcta gcatcgttaa tatggatgaa gtatttcgaa taaaagtttg tctgctaggc
    17641 catccgcgag ctgcttttcc caataagaaa aagtagctcc caaatgctag tacagtgata
    17701 cctgtttata cggatagcag atcgaatacg tgatgaggat ttttgttata tatcatcatt
    17761 ccccaccaac ctaaactgaa caacaaaatg aaggacaata atatagagta gattgttttt
    17821 ttcctttggt ctttccaatc aaacatgaaa taacttccta atgcatgaac gggtgtatcg
    17881 taataagtac agataacttt ctctgctttt tcgatatctc caacataaat attttttgat
    17941 cggtaggcta gcgtatcgta agcaatgact gtcacatctg ttctcatact gtaaatatct
    18001 gatactaatg ctgaaagaaa acgctgttta ctcttttttg tacggcgctt acggtaaata
    18061 tagcgataac gaaattccca atcacgaaac agctcgttct tcttcatttt ttcgccctct
    18121 ttatcttttt tctaagcatt tgtttatgac aaatcaaact tgttcaatac atcttttaat
    18181 cctgttttag gtgcagatgg agtggcttga aaataaatat tctttacgcc atattgttca
    18241 ttcatttctt tcaatttttc agcatctgat ttatttaaat aaacagattt cgtcactaaa
    18301 agatctgtat caggattttt ttgagcaatc cctccaatat tcaattcttc catggggatt
    18361 ccgtgtttta ctaagtcgta aatcgtttgg actgtttttg cgatcaaaat acaattgtat
    18421 tctttaaatt gatattcatc ccaatagaat ttagctttct ctggtgtaat gacaattgtt
    18481 ttttgtcctg tacggcttcc tgcatttttg taaagatctc tcataaattt atcttttgct
    18541 acaacttcgt ctacaacaaa aatggaattc actccacttt tttgagataa cgttaccatt
    18601 acttgcccat ggatcaaacg ctcatccaca cgcgctaaat tgactacacc catcttattc
    18661 acgttccctt ctttttataa aataccgatt cctgctagca caattaatat tccagtcagc
    18721 cataataatg cttgtgtgac tttcaatcct tttttcgtat agaacaagta cacacccatt
    18781 acgacagcta aaggtaatat ccctggcacg atttgatcaa gaatttcctg gataacaaac
    18841 tcacgtccgg atatcgcaaa tttcaatgaa gattctactt ttacatagtt tcccgcaagc
    18901 acccccatca taaacaaccc taaaactgat agcatttcaa tcgctgtctg aacaccagtg
    18961 ttttgcttta ggcctgtagc attacggccc atagtgaaag cttgtcttgc gaaaaatacc
    19021 cctagtaaaa cctgatacaa cccaaagcag ataaaaggaa aaagtgcgcc taaaggactt
    19081 ccctgttgtg cccaaggtac cgcgattgcg ataaaaatat attggacggt tcctgaatcg
    19141 attgcatctc cgattcctgc tagtgccccc atcaatcccg ctttcgtgtt atacatcaag
    19201 tcatcctgca tcaagatctc tttttcttca tactcttctt ctgctctttg ctgttccata
    19261 gaagacatta ggccagtaat cacaccgcca ccccagctta attgagtgtt gaagaacagt
    19321 aactgccgtt tatatgctgc ttttaaagag tcttcatctt tataaagttt tttcaagatt
    19381 ggcatcattc cgtatagtag tgatggcgcc aagtaacgtt caaaagagtg aggaatttcg
    19441 tttgcaaaat gccatctcag ataggctttt gttacatctt ttttcgtgat ttcttcagga
    19501 gcacctgttt gattatccat tatttcagcc atttcccatt cctcctaaat taaaatccgt
    19561 catcatagtc atcttcgtca tcaaaagatg ctgaattgtt attgcttgtg gcaggctgta
    19621 cttgtttccc tttttgcgac tggacaaaga tcaaagcgat aataatccca aaaatggcat
    19681 aagtaaccat cgtaatatct aaagatttca aaacaacact cataaaataa gcaaggaaga
    19741 aaaagaccag ataatctttt cgaccgataa cgtatacagt tgtcgcgata ccgattgccg
    19801 ctaaccctcc acctactact tcaagaatgt ggataacgac tgttccctct aaagctttga
    19861 ttacatcagc gatcaatggc gcccctaaat ataaagcaat gaaaacaagc ggaacgaata
    19921 ataaaaatga ggcaaccgtt ggataaagaa taattgatcg ggccatagct tttgtattca
    19981 aatcttctac agctttgtct gtatatttac ctaaaaaagt attgatgaaa aaacgaaact
    20041 gatacaagta acttccgagc aatccgactg gcaccgcaac ggcaatcgct gcttctggcg
    20101 acagatcccc taataatgcg actggtactg cgatagctgc tgcaatagat ggttcagctg
    20161 gcattgatcc tcctggagaa aaaaccccca tataaatcat ttgcaaagcg gctgtaacaa
    20221 tcatcgcttg tgcaacttcc cccatgatca acccgacgac taatccagtc atcaaaggag
    20281 aaaaacgtaa ggtcaacgtg gctcctccac ctaaccagcg ggacataacc gcggctaccc
    20341 ataaagcaat caacaaactt tgtactacac ttaatgtttc catgtgtcct cctccttcag
    20401 atctttctga tataaccttc cagttccatc attgactgta tcagtgattt ctgtgttacc
    20461 tctataggaa tcaaatgcac tttctctttc gaatcaataa attcagccat tttatctatt
    20521 atctgtttat ctttcggaaa taatcccatt tgttctagag aaaccggcag ttctaattcc
    20581 cgatacaaag gatataactc gtctattttt tcccagcgac cttctagtgc cagttgatag
    20641 aaaatcccat acgctacttt ttctccatgt agaaaacgat ggctttccgg aatatattta
    20701 ctgatagcgt catgcatagc atgtgctgcc acatttcttg cgtatttatc tcctaatcct
    20761 ccaactaaac cagcaacagc aaagataatc tctgaaagct gtttgaactc cgatgtcagt
    20821 tttctttgtg ccatatcttg gattgcttta gcagaatgat tcaaaatctc ttctttgcag
    20881 atcttcgcag tatcttgtgc tagcttcaaa aatgattcat cttgcaaaaa atcttgttct
    20941 aagatgagct cagattcata ccattttgct agtgtatctg ctaacccggc aatgaaatat
    21001 ggaacagggg aatcaatcac taattgggga tctgttataa aaaaagcagc ttgtctgtaa
    21061 aagtgttctg ttttcccttc tgcttgtcca ttctccttat acatgacagc aagaggtgtc
    21121 catggagcac aattactagc taaagtaggg atgactccga aatcgattct tgcaagattg
    21181 gcactataac ccactagatc ggtcagtttt cctccgccaa cgccaactac gaaatcgatt
    21241 tcattttttt gatgattgta gctattcgtc tggcttcagc ataactacat tctccagaat
    21301 aacgatgata aaaaaattga atcgtttcgt cattcaaaaa tactaattta ggtagcgcct
    21361 tttcccatga taccgttccg tgggtgacca gtactctttt cgcgccgtat gatttcaaga
    21421 gatcagaaac ttttgaaata ccaccttctt catataaata aaattgaggg cctgttttca
    21481 cttttaaacc gttcaccatt ttcttcatca tccttttata tagttgctct ttgtctggac
    21541 agaacgttca ggaagaaggt gctgactgat ctgttcgaca tctctgacac atttttcacc
    21601 cattgcttct aaacattccc ttgtgtaggc cgatatatgt ggcgtaatca ctacttgttc
    21661 aaacgctaaa tacggatgat cttgacgtcc cggttcttct tctaaaacat ctgtggcata
    21721 tcctgcgatc tttccgcttt tcagaccatt aatcatcgct tcttcatcaa ctagagcgcc
    21781 tcttgcacta ttagatagat aaacattttc tttcatctga tcgacttctt tatcagaaat
    21841 catgtgatag ttctcttctg tcaaattcgc acataaacaa atgatatctg attgttccag
    21901 taattcttga agttctactt tttttgcacc aaagctttcg atgtataaat ctgatttgta
    21961 aggatcataa gctagaacat cacagcgaaa accgttacgc aaaatttcga ttactccgct
    22021 tccggtattt cctgcaccaa tcacaccaac tgttttgtta aacagcgtcc taccgacgaa
    22081 ctccgcccga tcttcccatc gatcgtcctt gactcttgct tgtgcttgtg tcgtgcgtcg
    22141 aagcatggac aacaaattag ttatattatt ttctgctaca gcatttcgtt caactaaagc
    22201 aggtatgata gatacgattg tctggtgttc ttttgctgct tttaaatcaa tattgttgta
    22261 accaatacca tgtcgggaaa tcaatagcag ttcatctttg ttttcaaaaa aatctcgtcg
    22321 aaaaaatggc gtaacacttg caataatgat attgaatcct ttaagtaact ccgctagttc
    22381 ttttcctgga atctcttgat caacagtaaa gaaatgaacg tctccaactg actttaatct
    22441 ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa ttaacgattg ctatatgata
    22501 atctggcatt ttactcaccc ttttcaacta tttcatgtcc gccaaaccca tttcttagtg
    22561 ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc atgaacaaac
    22621 tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc gtccattttg
    22681 cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt tcgtttaact
    22741 gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc caaacacgtg
    22801 cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat ccttcaccga
    22861 ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag tgtccgcttc
    22921 cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat aattgttcca
    22981 cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt cgtgctcctt
    23041 cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga aactcttctg
    23101 ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg tctcctggtc
    23161 cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca ggaagcatta
    23221 accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct tgataagcag
    23281 gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt ccttgtactt
    23341 tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc aaaccaatta
    23401 caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa aaaatttttt
    23461 tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc cttgtatctt
    23521 taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga attctcatga
    23581 gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca aaagaacagg
    23641 ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct attagcaatt
    23701 tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa aaacttggtt
    23761 ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat ctatctgcta
    23821 tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg caaaaggtgt
    23881 tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa aaggatctaa
    23941 aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt gttggcggtt
    24001 cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc gcaacttctc
    24061 acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa aaagactgtg
    24121 ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct gaaaccgtaa
    24181 gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc cttgctaaac
    24241 ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct gaagctttag
    24301 cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg atgttttaca
    24361 acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct gaaatcaaag
    24421 aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca ctaccaaata
    24481 tttggtagtg gcttttttgg ttctgcaagg gtggtacaga
//
LOCUS       DACECJ010000036        24464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.036, whole genome shotgun sequence.
ACCESSION   DACECJ010000036 DACECJ010000000
VERSION     DACECJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.036"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IWO09_001512"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IWO09_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0264104.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1552..2973)
                     /locus_tag="IWO09_001513"
     CDS             complement(1552..2973)
                     /locus_tag="IWO09_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0264105.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IWO09_001514"
     CDS             complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IWO09_001514"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0264106.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5384..6640)
                     /locus_tag="IWO09_001515"
     CDS             complement(5384..6640)
                     /locus_tag="IWO09_001515"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0264107.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7220..8521
                     /locus_tag="IWO09_001516"
     CDS             7220..8521
                     /locus_tag="IWO09_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0264108.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8590..9189)
                     /locus_tag="IWO09_001517"
     CDS             complement(8590..9189)
                     /locus_tag="IWO09_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0264109.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9443..10324
                     /locus_tag="IWO09_001518"
     CDS             9443..10324
                     /locus_tag="IWO09_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264110.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10337..11947
                     /locus_tag="IWO09_001519"
     CDS             10337..11947
                     /locus_tag="IWO09_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0264111.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12024..12683)
                     /locus_tag="IWO09_001520"
     CDS             complement(12024..12683)
                     /locus_tag="IWO09_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0264112.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12966..13631)
                     /locus_tag="IWO09_001521"
     CDS             complement(12966..13631)
                     /locus_tag="IWO09_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264113.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IWO09_001522"
     CDS             complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IWO09_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0264114.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IWO09_001523"
     CDS             complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IWO09_001523"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0264115.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IWO09_001524"
     CDS             complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IWO09_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0264116.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15945..16265
                     /locus_tag="IWO09_001525"
     CDS             15945..16265
                     /locus_tag="IWO09_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0264117.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16377..17246)
                     /locus_tag="IWO09_001526"
     CDS             complement(16377..17246)
                     /locus_tag="IWO09_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0264118.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17342..18616)
                     /locus_tag="IWO09_001527"
     CDS             complement(17342..18616)
                     /locus_tag="IWO09_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0264119.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18642..19151)
                     /locus_tag="IWO09_001528"
     CDS             complement(18642..19151)
                     /locus_tag="IWO09_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264120.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19326..21461)
                     /locus_tag="IWO09_001529"
     CDS             complement(19326..21461)
                     /locus_tag="IWO09_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0264121.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21544..23199)
                     /locus_tag="IWO09_001530"
     CDS             complement(21544..23199)
                     /locus_tag="IWO09_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0264122.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23201..23515)
                     /locus_tag="IWO09_001531"
     CDS             complement(23201..23515)
                     /locus_tag="IWO09_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0264123.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23685..23945
                     /locus_tag="IWO09_001532"
     CDS             23685..23945
                     /locus_tag="IWO09_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0264124.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23959..24273
                     /locus_tag="IWO09_001533"
     CDS             23959..24273
                     /locus_tag="IWO09_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0264125.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6972 a         5059 c         4410 g         8023 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag acgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt tttatttttc
     1561 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
     1621 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
     1681 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
     1741 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
     1801 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
     1861 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
     1921 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
     1981 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
     2041 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
     2101 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
     2161 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
     2221 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
     2281 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
     2341 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
     2401 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
     2461 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
     2521 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
     2581 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
     2641 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
     2701 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
     2761 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
     2821 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
     2881 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
     2941 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
     3001 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
     3061 gaggggattc tttgagtcta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
     3121 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
     3181 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
     3241 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
     3301 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
     3361 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
     3421 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
     3481 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
     3541 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
     3601 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
     3661 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
     3721 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
     3781 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
     3841 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
     3901 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
     3961 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
     4021 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
     4081 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
     4141 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
     4201 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
     4261 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
     4321 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
     4381 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
     4441 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
     4501 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
     4561 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
     4621 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
     4681 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
     4741 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
     4801 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
     4861 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
     4921 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
     4981 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
     5041 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
     5101 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
     5161 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
     5221 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
     5281 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
     5341 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
     5401 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
     5461 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
     5521 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
     5581 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
     5641 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
     5701 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
     5761 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
     5821 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
     5881 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
     5941 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
     6001 acgcttctac ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
     6061 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
     6121 agtcgataga ttgcagaatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
     6181 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
     6241 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
     6301 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
     6361 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
     6421 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
     6481 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
     6541 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
     6601 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
     6661 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
     6721 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
     6781 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
     6841 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
     6901 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
     6961 attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt
     7021 ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca
     7081 attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact
     7141 ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa
     7201 aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc
     7261 tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa
     7321 agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa
     7381 gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat
     7441 tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac
     7501 actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt
     7561 ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg
     7621 atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca
     7681 aaatgatttt gttttagtta atttaaatga tttgatccaa aaagacgaac aaggaaatct
     7741 ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga
     7801 tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa
     7861 taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga
     7921 catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc
     7981 aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc
     8041 tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga
     8101 acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac
     8161 tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc
     8221 tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca
     8281 gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt
     8341 ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa
     8401 tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat
     8461 tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg
     8521 attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
     8581 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
     8641 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
     8701 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
     8761 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
     8821 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
     8881 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
     8941 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
     9001 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
     9061 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
     9121 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
     9181 accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt
     9241 tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt
     9301 atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaagt
     9361 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
     9421 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
     9481 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
     9541 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
     9601 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
     9661 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
     9721 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
     9781 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
     9841 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
     9901 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
     9961 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
    10021 cgagcgacta gcggataaag tcgtgatcat acgcgaagga acagtcgtag aagaaggaac
    10081 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
    10141 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
    10201 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
    10261 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
    10321 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
    10381 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
    10441 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
    10501 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
    10561 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
    10621 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
    10681 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
    10741 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt
    10801 ccaagcagat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
    10861 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
    10921 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
    10981 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
    11041 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
    11101 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
    11161 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
    11221 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
    11281 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
    11341 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat
    11401 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
    11461 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
    11521 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
    11581 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
    11641 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
    11701 cggatggctt cctcgaatgg gtaaacttgc ttatatctac ttagcttact cctttgcact
    11761 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
    11821 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
    11881 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
    11941 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
    12001 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
    12061 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
    12121 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
    12181 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
    12241 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
    12301 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
    12361 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
    12421 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
    12481 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
    12541 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
    12601 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
    12661 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
    12721 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
    12781 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
    12841 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
    12901 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
    12961 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
    13021 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
    13081 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
    13141 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
    13201 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
    13261 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
    13321 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
    13381 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
    13441 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
    13501 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
    13561 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
    13621 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
    13681 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
    13741 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
    13801 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
    13861 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
    13921 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
    13981 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
    14041 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
    14101 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
    14161 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
    14221 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
    14281 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
    14341 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
    14401 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
    14461 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
    14521 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
    14581 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
    14641 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
    14701 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
    14761 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
    14821 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
    14881 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
    14941 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
    15001 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
    15061 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatccg
    15121 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
    15181 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
    15241 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
    15301 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
    15361 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
    15421 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
    15481 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
    15541 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
    15601 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
    15661 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
    15721 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
    15781 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
    15841 attaatgaac aattcaagta tataataaat ttctaatttt gggaagatat gtgtagtgct
    15901 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
    15961 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
    16021 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
    16081 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
    16141 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
    16201 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
    16261 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
    16321 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
    16381 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
    16441 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
    16501 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
    16561 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
    16621 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
    16681 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
    16741 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
    16801 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
    16861 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
    16921 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
    16981 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
    17041 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
    17101 gcaccagaag agcttatagt tgttgaaggt tctgtttgag caggttcaga agtttccgca
    17161 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
    17221 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
    17281 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
    17341 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
    17401 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
    17461 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
    17521 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
    17581 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
    17641 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
    17701 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
    17761 tcagccagac ttctgttcca ccttttgccc aacgttttct ttgtcgatat agcatcttca
    17821 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
    17881 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
    17941 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
    18001 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
    18061 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
    18121 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
    18181 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
    18241 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
    18301 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
    18361 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatcttcga
    18421 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
    18481 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
    18541 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaataca taatagacaa
    18601 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
    18661 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
    18721 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
    18781 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
    18841 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
    18901 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
    18961 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
    19021 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
    19081 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
    19141 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
    19201 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
    19261 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
    19321 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    19381 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    19441 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    19501 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    19561 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    19621 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    19681 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    19741 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    19801 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    19861 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    19921 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    19981 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    20041 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    20101 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
    20161 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
    20221 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
    20281 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
    20341 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
    20401 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
    20461 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
    20521 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
    20581 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
    20641 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
    20701 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
    20761 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
    20821 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
    20881 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
    20941 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
    21001 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
    21061 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
    21121 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
    21181 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
    21241 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
    21301 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
    21361 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
    21421 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
    21481 taaagtggtg gacccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
    21541 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
    21601 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
    21661 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
    21721 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
    21781 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
    21841 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
    21901 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
    21961 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
    22021 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
    22081 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
    22141 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
    22201 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
    22261 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
    22321 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
    22381 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
    22441 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
    22501 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
    22561 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
    22621 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
    22681 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
    22741 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
    22801 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
    22861 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    22921 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    22981 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    23041 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    23101 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    23161 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    23221 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    23281 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    23341 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    23401 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    23461 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    23521 gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga gctttattaa
    23581 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    23641 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    23701 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    23761 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    23821 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    23881 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    23941 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    24001 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    24061 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    24121 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    24181 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    24241 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    24301 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    24361 aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
    24421 catatttttc gtttggttgg gcttattacc gcagatgcaa gctt
//
LOCUS       DACECJ010000037        23830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.037, whole genome shotgun sequence.
ACCESSION   DACECJ010000037 DACECJ010000000
VERSION     DACECJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.037"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWO09_001534"
     CDS             complement(55..789)
                     /locus_tag="IWO09_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0264126.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IWO09_001535"
     CDS             1116..1526
                     /locus_tag="IWO09_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0264127.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IWO09_001536"
     CDS             1519..2220
                     /locus_tag="IWO09_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0264128.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IWO09_001537"
     CDS             2220..3806
                     /locus_tag="IWO09_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0264129.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IWO09_001538"
     CDS             3825..4046
                     /locus_tag="IWO09_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0264130.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IWO09_001539"
     CDS             4043..4804
                     /locus_tag="IWO09_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0264131.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IWO09_001540"
     CDS             5184..5693
                     /locus_tag="IWO09_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0264132.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IWO09_001541"
     CDS             complement(5768..6307)
                     /locus_tag="IWO09_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0264133.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWO09_001542"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWO09_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0264134.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IWO09_001543"
     CDS             7374..8171
                     /locus_tag="IWO09_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0264135.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IWO09_001544"
     CDS             8199..8834
                     /locus_tag="IWO09_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0264136.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IWO09_001545"
     CDS             8854..9627
                     /locus_tag="IWO09_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0264137.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IWO09_001546"
     CDS             10035..10832
                     /locus_tag="IWO09_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0264138.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IWO09_001547"
     CDS             10810..11223
                     /locus_tag="IWO09_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264139.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IWO09_001548"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IWO09_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0264140.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IWO09_001549"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IWO09_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0264141.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IWO09_001550"
     CDS             16000..16560
                     /locus_tag="IWO09_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0264142.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWO09_001551"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWO09_001551"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0264143.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IWO09_001552"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IWO09_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0264144.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IWO09_001553"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IWO09_001553"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0264145.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IWO09_001554"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IWO09_001554"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0264146.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IWO09_001555"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IWO09_001555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0264147.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IWO09_001556"
     CDS             21907..22485
                     /locus_tag="IWO09_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0264148.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IWO09_001557"
     CDS             22537..23019
                     /locus_tag="IWO09_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0264149.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IWO09_001558"
     CDS             23136..23510
                     /locus_tag="IWO09_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264150.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6648 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt
//
LOCUS       DACECJ010000038        23637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.038, whole genome shotgun sequence.
ACCESSION   DACECJ010000038 DACECJ010000000
VERSION     DACECJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.038"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..279)
                     /locus_tag="IWO09_001559"
     CDS             complement(<1..279)
                     /locus_tag="IWO09_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0264151.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFN"
     gene            complement(304..735)
                     /gene="ruvX"
                     /locus_tag="IWO09_001560"
     CDS             complement(304..735)
                     /gene="ruvX"
                     /locus_tag="IWO09_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0264152.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(732..998)
                     /locus_tag="IWO09_001561"
     CDS             complement(732..998)
                     /locus_tag="IWO09_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0264153.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1253..1978)
                     /locus_tag="IWO09_001562"
     CDS             complement(1253..1978)
                     /locus_tag="IWO09_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0264154.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2339..2409)
                     /locus_tag="IWO09_001563"
     tRNA            complement(2339..2409)
                     /locus_tag="IWO09_001563"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2375..2377),aa:Gly,seq:ccc)
     gene            2526..3278
                     /locus_tag="IWO09_001564"
     CDS             2526..3278
                     /locus_tag="IWO09_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0264155.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3294..3773
                     /locus_tag="IWO09_001565"
     CDS             3294..3773
                     /locus_tag="IWO09_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0264156.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4024..5604)
                     /locus_tag="IWO09_001566"
     CDS             complement(4024..5604)
                     /locus_tag="IWO09_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0264157.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5597..6340)
                     /locus_tag="IWO09_001567"
     CDS             complement(5597..6340)
                     /locus_tag="IWO09_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264158.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6516..7292)
                     /locus_tag="IWO09_001568"
     CDS             complement(6516..7292)
                     /locus_tag="IWO09_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0264159.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7540..8376)
                     /locus_tag="IWO09_001569"
     CDS             complement(7540..8376)
                     /locus_tag="IWO09_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0264160.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8387..9316)
                     /locus_tag="IWO09_001570"
     CDS             complement(8387..9316)
                     /locus_tag="IWO09_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0264161.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9508..9861
                     /locus_tag="IWO09_001571"
     CDS             9508..9861
                     /locus_tag="IWO09_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0264162.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9935..10591)
                     /locus_tag="IWO09_001572"
     CDS             complement(9935..10591)
                     /locus_tag="IWO09_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0264163.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10817..12751
                     /locus_tag="IWO09_001573"
     CDS             10817..12751
                     /locus_tag="IWO09_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0264164.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12837..14060)
                     /locus_tag="IWO09_001574"
     CDS             complement(12837..14060)
                     /locus_tag="IWO09_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0264165.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14054..15952)
                     /locus_tag="IWO09_001575"
     CDS             complement(14054..15952)
                     /locus_tag="IWO09_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0264166.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(15946..17064)
                     /locus_tag="IWO09_001576"
     CDS             complement(15946..17064)
                     /locus_tag="IWO09_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0264167.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17147..17980)
                     /locus_tag="IWO09_001577"
     CDS             complement(17147..17980)
                     /locus_tag="IWO09_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0264168.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(17973..18890)
                     /locus_tag="IWO09_001578"
     CDS             complement(17973..18890)
                     /locus_tag="IWO09_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0264169.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19117..20241)
                     /locus_tag="IWO09_001579"
     CDS             complement(19117..20241)
                     /locus_tag="IWO09_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0264170.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20424..21332
                     /locus_tag="IWO09_001580"
     CDS             20424..21332
                     /locus_tag="IWO09_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264171.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21473..23086
                     /locus_tag="IWO09_001581"
     CDS             21473..23086
                     /locus_tag="IWO09_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0264172.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7372 a         4617 c         3949 g         7699 t
ORIGIN      
        1 attgaatact tcttcaatca tgtcccattc tgcgtctgtt tcgatttgtt ctaactcgcc
       61 ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt aattctacat cttcatcttc
      121 tgaaacacca gcaggatata aaagaacata gttacgtcca aattcttctt gtccgtcaac
      181 tgttagtagg atttcatata atgtttcatt tccttggtca tccaccaatg tgatgtgttc
      241 gtgtccttca tggtcgtggg aatggttatg ttctgtcata agattacctc ttttctttta
      301 ttattaaaat aattgcttac ctgagccgtc taaataattt tgtaagatca tcacagctgc
      361 taatttatcg atgacttttt ttcgtttggc tcttgaagta tttgcctgct ctactagcat
      421 acgttctgcc tgaacagtgg ttaatcgttc atcttgataa tcaactggca aggagaatag
      481 ttctttgatc ttttctccgt atgacatgga agcttctgcc cttggtccga tggaattgtt
      541 catattttta ggtaatccaa ctacaaaccg gtctacttca tattgtgcta ctaattcctt
      601 caaccgatca aagccaaact cttcttctgc ttcattgatc cgaatgatct ctactccttg
      661 agctgtccag ccaaaggggt cactgatagc aacacctact gtcttagaac cgacatccaa
      721 gcccattact ctcataaatt cactccatga tttgagaggt agtccttgac aattacttcc
      781 atgatctcat cgcgttcgtg acggcgaatc aaatttcgag catcacgata acgaggaatg
      841 taggccgggt ctccagaaag caagtagccg acaatctgat tgattggatt gtagcctttt
      901 tcttccaaag cccgataaac aatcgctaat gtttcgctca cttctttttt gcgactatcg
      961 tcaaaaccaa aacgtacggt ttcatctgta aaacccatga ttctacacct ctttcccgca
     1021 aggtaattcc agcttacctt ctacaacctt attttactcg aagaaaggga aaactacaaa
     1081 caaatattcc aaacttccga ataaattaag aaacactatt tgtatgtttt tttccttttt
     1141 tctgtagctt tatcaaaagt actcttttca taaagtatac gctatatttc tttgaatgta
     1201 tgtttttaca caattttttc ataatattca taaaaaattc gaatagctgc tactaatttt
     1261 ttgtttttgt tgcttgtcct gtctcataat tgatcgtgta gccatcttga acattgtaga
     1321 taaagacatt gaattcaatt tttttgtctt caatgctttc tgcttccaat tgcactcccc
     1381 tggctaccag ttcattcccc tcaaaatgag gagtgacacg gtagcgtaca tgatgatttg
     1441 tttctttgat atatgccgca atatcatttt cgtgagcaag catgtatgga gcattcaacg
     1501 aacgtgtccc tgtcatcaag tttttaatgt tgttattttg accagtcagc tggaaaccaa
     1561 ttaaatggct gcgattatat aaccattgcc cgtcacttaa tttcttttgt ttccaacctg
     1621 tcgggtcaat atacaaaggc tctctctttt cagtagggaa caaatctttt ccaagcatag
     1681 cattggcttg tcctactctg ttcaatttat caatatttga atagctttcc catgttcctt
     1741 tggaaaggct aagatcctct tttgtaaatt ccggctgatt cccattgatt tctatttctt
     1801 gatgttttcc atcatattct ggcacatccg ctacttgaga agcttgtggt gtggaatcaa
     1861 aaatagttgt aatctgctga aagatatctg aatctttttg tgtggtggaa acaccgaatg
     1921 cccctgcaat cacagcagcg acgatcccca cgatcgcctt ggttgttttt ttgctcattt
     1981 ctttactcct ttactaacag ctccctaaat cttaacatat gttcccctat aaataaatac
     2041 tagaaagaaa tagaaacagc aaaaaataaa acctgtttta agatggctta aaaatacaaa
     2101 aaagagacgg attcctcgtc tctaatcagt caccactcta tctgtaaggt atttattatt
     2161 attcaaaaat tttcttcata ctcaagacaa tacactgatt tcacaaccgc ttggtaaacg
     2221 gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa gttaaaaaca taagaagttg
     2281 tttttgcttg gctgttctta ttacttgttg cagagcagct ttttcacaac ctattttaag
     2341 cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag ctgggatttt accactaaac
     2401 tacacccgca atacaaaaac agtatacgcc tcttttattc cttcctcaag tttttttatt
     2461 ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa tcaatcaatt attggagaag
     2521 atgctatgac gggaaaagaa ttagatctgt tgctggacac ttgtttactc gctgggaaaa
     2581 tcatgatgga aagcaatgcg gagatgtatc gggtagaaga tacgatgagt cgaattgctt
     2641 tagcttctgg aaatttccgt ttggtcagct atgtcactca aaccggtctc tttattggtc
     2701 ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa ccgttcgatt aatttagaaa
     2761 aagtagtaaa agtaaatgat ctttcacgta aatatgttgc ttcagagcta acattagaag
     2821 aactttactg cgaactgcaa gcaatcgacc gagaccgttc tttctttcct gtctggttgc
     2881 aaatcgttag cgcggcagtg atcagtggaa caattatggt gttatttggc ggaaagatga
     2941 ccgatttacc tgtgacttta gtcattggag gattgggata cgcagcttat ctatatagtt
     3001 tgaaattttt tagaatcaaa tttttatcgg aatttttatc ttctttattg attggcatag
     3061 ccgctatcta cagcgtacgt tttggcttag gaagcaatca ggatctaatc attatcggat
     3121 gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc tatccgtgat ttgctagctg
     3181 gacattatgt ctcaggagtt tctcgcggaa cagaagcgat gatgactgct acgatgatcg
     3241 gttttgcgat tgcttttatt ttccagcttt tttattagga aaggaacaca agtatgggga
     3301 cgatatttat tcagttttct tttagttttt tagcgactgc ggcatttgcc gttattacaa
     3361 atgtacctag gcgttcattg atttgttgtg gtctttctgg tgcttttggc tggatgatct
     3421 actggatctg tgttcaaata ggcggatctg ctgcttttgg ttcattgctt ggttctttag
     3481 gagtagcatt catcagcgat cttttctcta aacgtttgaa aatgcccgta acgattttca
     3541 atattcccgg gatggttcct ctagtccctg gtggtttagc ttatcaagcg gttcgaaatc
     3601 tcgttactga atcctatcga gaagcggctc attatgcggt ccaagcaatt atgattgctg
     3661 gagcaatcgc tttaggtctt gtattatcag aagtctttaa ccataacatc cgtaatttca
     3721 aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg gctttttaaa taaacgaagt
     3781 gtaacaaaga aaaggttgtg aaagagacgt tcagccttga gcgatgacaa aatatctcac
     3841 aaacattgcg acaagtaacc gcaggagcaa taagaagtaa ttatgagtat taaaaaaagt
     3901 tttgtaaaaa tagcttctca catttttaca taaaattgac ttaatactga gaaactgact
     3961 tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg atttttgtca cggcccctct
     4021 tctttattca aattttttcc aaaacgttcg ttgataataa acgatccata gagcggaaag
     4081 tattagaact aaaccgatca caggaatatt cactaaccaa aatggataaa acaaagccaa
     4141 cattccgtat aagacgataa gaataatact gatgatcaat gaacccatca tagttgcgac
     4201 taaacccaaa cttcctgaac ctctgttaaa taattggtta atattggtcc agttcagcag
     4261 cagcaaccga taatctctag cgaaataacg tagtgacaat aagtagcagc cgaataaaga
     4321 accaaatagc aagctaggga taaaaaacaa aggcagctga aaagacagtc cgcttaataa
     4381 tgcgatccca ccagttaaaa aactctgcaa tatccatccg aatctgaact tttcttttaa
     4441 atattggttc atagagattg gcaaagaacg gataaacaga aaattttctt gatccaaaga
     4501 aataagattg ctgataaaag aagtctgatt aactgttaag aaagccaatg cgatccctac
     4561 caggaaaaca acaccgatta acttattatc taaatcagtt aattttattt cgcctcctag
     4621 ggcaaaagca agaataaaaa tgattggagt taacaaggaa gaagaaagaa cttgcataat
     4681 caaattaggt tcttttacta attgagcatt gtaactaaat aaaagctgat gcacattttg
     4741 gttcttttta tgggttcttt ttattttgaa ctcactgttt gaatcagtca gttgctctgt
     4801 aagctttggt actatcagct gttttacagc aaggaaaaat cccagagccg tgatagctag
     4861 tattccccaa cccaataaac cttgttgaga aagtggctga ctggcagcat catagaatgg
     4921 caataatatc gaaatcggat gccgatctgt taactcattt tcaatagtcc ccgtttgatg
     4981 attcatccac atgattccag caaatgcgat cagcatcgag ctgccaagca agatactaga
     5041 aactgttttt ctatgttttt taaacactgc tgttcgagcc aaaccaaaaa caatcaaact
     5101 agccagacaa aaaagaataa tcaaaaatag agaaaacaaa aggattgaga taatgatagt
     5161 aagcaaaatc cctagatcag ctcgccatcc ggttaaaata aacatgacta gaagcggaaa
     5221 aacaaaagga atgatggtca aagcaactac caaaatcttt gctgaaaaaa cctgttcttg
     5281 tccaaaaggc agaggcaaaa atacttgcag gtcatttcct tcaaaaaaga tattataaat
     5341 gactgatata ctttgagtta aacctaacat accaaaaagt gccacgtaat aagtaaagaa
     5401 acctggcaat ttaacaaagt tcatagcaaa catagcaaat ccgtaaatca gtaaaaaaat
     5461 cagccctgaa aaaagatttt gagacaaaag atagatagtt aatcgctttc ccttttttcc
     5521 tttctctctt gctttattcg tgagctgtgg attaacatat aaaagattaa ctcgaattaa
     5581 ttctaataat tgtcgcttat acatacggtc catctcctgt cgcttgcctt ccagccattt
     5641 tcaaataaat cttttctagc gtttcatcag gtgagccatt caataattca ctaactgccc
     5701 cctgataaat caattcacct ttcttcaaaa tagccagttt atcgcataac tgttgtgcgg
     5761 tatccaatac atgtgtagaa aaaatgactg tttttccttt tgcggcatga gccttcatca
     5821 tttctttcaa atcaaatgct gcttgaggat caagtccttg caaaggttca tccaataccc
     5881 aaatatctgg atctggcagc aacgtgccga tcaaaatcgt tttttgacgc attccatgtg
     5941 aaaaacttgc gattgtttca ttttgatgac tatacatatc aaaaagcgct gtcaattctg
     6001 ctaaacgctt ttcttttttt tgtgtgtcta attcataggc agctgaaatc aagtcccaat
     6061 attctccagc cgtcaattgt aaaaaaatat ctggggaatc aggcacataa ccgatttttt
     6121 gtttgatact caatcgattt tccgtcaatt tcattccatc tactaaaatc gttcccgatg
     6181 atggttcaat aatactaact aaacttttaa tcgtagttga ttttccggca ccattatgtc
     6241 ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga caagtctttt aatgcttgtt
     6301 ttttcccgta acttttactt acattgatga attcaatcat ttctgtactc cttttgtttg
     6361 ttattacttt ttattgtacc cttcgttatt ggtggagtaa atgtcaaaaa gagtgcataa
     6421 caaaaaagga tagaaacgac taggctgcta cttgggttgt tctcttctac aggaaaacac
     6481 tcttctaaca ggtaagatat gagtctttgt tttctttaaa atgattcatt ttctatcttt
     6541 tcttgcataa aattctttag tttatcaata cttctctttc ccatctctag ttgttctcca
     6601 tttgcaaatg ttactatttc gtttactctg ctaatagatg taatgttttc agtattaatg
     6661 atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt atatttttta
     6721 attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt atttttataa
     6781 cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc gctattacca
     6841 taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt ttctttttct
     6901 ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc ttttatcaaa
     6961 taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg atgagacgtt
     7021 agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag ttcgataccg
     7081 ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata gatttcatta
     7141 tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc aaatttccaa
     7201 ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc aaaaaccaaa
     7261 ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg atattttact
     7321 atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg taattctctt
     7381 cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa
     7441 aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc
     7501 gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata
     7561 ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg
     7621 cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc attcatcatt
     7681 tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac
     7741 acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg
     7801 gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat
     7861 tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata
     7921 cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt
     7981 atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg
     8041 gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt
     8101 ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga
     8161 caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca
     8221 caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta
     8281 gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa
     8341 ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg
     8401 tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa
     8461 aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg
     8521 cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt
     8581 ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg
     8641 ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta
     8701 atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca
     8761 catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt
     8821 catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag
     8881 cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta
     8941 tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca
     9001 cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc
     9061 cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa
     9121 atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca
     9181 cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga
     9241 aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg
     9301 gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat
     9361 acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta
     9421 ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc
     9481 acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa
     9541 gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt
     9601 tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt
     9661 catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca
     9721 gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt
     9781 aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa
     9841 gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa
     9901 gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg
     9961 catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat
    10021 tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt
    10081 tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta
    10141 aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa
    10201 cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga
    10261 tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc aagttcccta
    10321 caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca
    10381 taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat
    10441 ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt
    10501 ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag
    10561 ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac
    10621 atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat
    10681 attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat
    10741 ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag
    10801 tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc attttggtgc
    10861 agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt
    10921 tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc
    10981 tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac
    11041 aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag
    11101 acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac
    11161 acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg
    11221 aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc
    11281 ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt
    11341 agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa cgaaccattt
    11401 agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt
    11461 gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag
    11521 cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga
    11581 acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga
    11641 agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag
    11701 aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa
    11761 ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc
    11821 tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga
    11881 agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat
    11941 tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta
    12001 ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga
    12061 tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc
    12121 tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc
    12181 tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca
    12241 tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact
    12301 gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact
    12361 ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca
    12421 ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc
    12481 cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca
    12541 acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt
    12601 ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa
    12661 ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact
    12721 aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg
    12781 ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac
    12841 caataagaag accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc
    12901 caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa
    12961 aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa
    13021 acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct
    13081 aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca
    13141 tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat aaaataattg
    13201 gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca
    13261 aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga
    13321 tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt
    13381 gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt
    13441 ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa
    13501 caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata
    13561 actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc
    13621 ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa
    13681 ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct
    13741 gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta
    13801 attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt
    13861 ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc
    13921 gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa
    13981 cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt
    14041 tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta
    14101 gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat
    14161 cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac
    14221 cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca
    14281 taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc
    14341 cagcttcact caccctatag ttttccttgt cttcttctat gattattcct tctttctcag
    14401 gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt
    14461 tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta
    14521 aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat
    14581 ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat
    14641 atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat
    14701 tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat
    14761 tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat
    14821 tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca
    14881 taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa
    14941 tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag
    15001 ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc
    15061 atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg
    15121 cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa
    15181 aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag
    15241 ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac
    15301 cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat
    15361 accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt
    15421 ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc
    15481 aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata atctggcttt
    15541 tttctcctgt atctaatttt ttccataata attgttcatg atgatataac gctaataaca
    15601 ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat
    15661 gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta
    15721 attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa
    15781 ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat
    15841 aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc
    15901 ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc aaatagctaa
    15961 ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg
    16021 cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc
    16081 atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc
    16141 atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc
    16201 agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg
    16261 aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc
    16321 tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc
    16381 atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata
    16441 tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag
    16501 atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt
    16561 ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc
    16621 aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag
    16681 taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg
    16741 tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta
    16801 cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt
    16861 tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat
    16921 tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt
    16981 ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga
    17041 ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg
    17101 gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag
    17161 ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag accattggga
    17221 tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag
    17281 aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga
    17341 ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa
    17401 tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat
    17461 cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa
    17521 acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc
    17581 ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag
    17641 atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat
    17701 aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca ataacaatcg
    17761 aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata
    17821 gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg
    17881 aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga
    17941 cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta
    18001 aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct
    18061 tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct
    18121 gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag
    18181 ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt
    18241 aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct
    18301 ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa
    18361 atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg
    18421 ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg
    18481 aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa
    18541 aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc
    18601 atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac
    18661 aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca
    18721 attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag
    18781 aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct
    18841 tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc
    18901 tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc
    18961 ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt
    19021 caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt
    19081 gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc
    19141 tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc
    19201 taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt
    19261 ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc
    19321 ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa
    19381 agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt
    19441 ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa
    19501 ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa
    19561 agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa
    19621 ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc
    19681 ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata
    19741 acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg
    19801 acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata
    19861 ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc
    19921 tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg
    19981 tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt
    20041 gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata
    20101 tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc
    20161 gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac
    20221 caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt atataaaacg
    20281 ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa aaaatatttt
    20341 acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa tagaaagctt
    20401 tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa tcctccaatc
    20461 ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct gctgctccct
    20521 attttcgact actaataaat gaaagcgcta atttatatgc tgaatttgca cataaatcta
    20581 tagaactaga taatccttcc cttttattca ttgataaaca gcaagaagtc caaatctttt
    20641 ctactcaaaa ggaaacacaa acttttatca ttatccagtt ttattttgat gagcaattat
    20701 tagcacatat tacgggacaa tctttttcta tagatcaaga aataacatct atcattactt
    20761 ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca tagtaagcca gctatttcct
    20821 atgatcagga aatcaattct tttttcatac tatgccaaca gatgagcatt catttgcttt
    20881 ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc ttccttacca aggcattatc
    20941 aacaggaact tgctttattg acaatacctt caaatgagaa agtcattcct aaaaaaaagc
    21001 atattcttta ttcagatgaa ttagttgatc caatcatacg atatttagaa gaacatcttt
    21061 cagaaaattt tttgcttgaa caattagccc aaattttttt tatcagtcca gccagcttaa
    21121 aaaaacattt tagaaaagtg aatggaaaaa gtgttattag ctattttaaa gaactaaaaa
    21181 tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac ctatacagaa attgcagata
    21241 tattaggatt tcattcgatc catcattttt cttcagcttt taaaaaatat acagggttat
    21301 ccccttcaaa gtattatcta tccttaaaat agaaaaaaat ttttaggttt tatccacaaa
    21361 ttatttctct atattttctg tctattcaaa ataaacaata attttccggc acaattattt
    21421 gctgtttttt accataacat agtacgatca acgtagaaaa ggagatgttt ttatgaaagt
    21481 tgttgtcatt ggcggtgtag ctggcggtcc ttcatttgcc actcgtttcc gtcgattgaa
    21541 tgaagcgcac gaaatcatta tctatgaacg cggagaaaat atttcttatg caagttgtgc
    21601 tttgccttat tatttaggag gtgtgatcac tgaccgtgac tcgctgatcg aacgtacacc
    21661 agaaatattg aaaacaaaaa acaacatcga tgtctttact aaacacgaag taacagcaat
    21721 cgatccttct actaagcgat taacagttaa agacctatcc acaaatgaag aaataaaaac
    21781 agattacgat aagttgatca tctcttctgg tgctagacca gattatccgg atattcccgg
    21841 agtttttgaa gcagaaaacg gctttgtcct ccgtagtgtg acggatgcgg atcgaatcaa
    21901 atcgttcctt gaagaaaaaa atccacaaca tgtcgtcatt cttggtgcag gtgttatggg
    21961 tctggaatta gctgagaatt tcaagcatcg cggcttagac gtgactttaa tcgatcaatt
    22021 gccacaagtc gctttccctt atgatccaga aattgctaat ttagtttatg acaaattgct
    22081 gaaagaagga ttagccgttc atttagaaac aagagttact gagatccgtg ataaaggtcg
    22141 agaaatagta ttatcagatg gctccgtcct ttctgctgat atgctaattt ttgctgttgg
    22201 tgtttctccg aagaatgaag tggtgaaagc aaccggcata caattatcag atacaggtca
    22261 gatcattgtt gatgaccagt tacaaaccaa tctgccggac atctatgcga ttggcgatat
    22321 tatcgaaacg actagtgtag tgactggtca gccgatccaa agtatgcttt ccagtgcggc
    22381 caatcgtcaa ggacatatgc tggcagatat tttaaatggt acgcctatgc gctatcgcgg
    22441 atatattggt gcaggtgtcg caaaaatctt tgatcataca gcaagttatg cgggaatgac
    22501 agaacatgaa ttaaaagcat caggcataac aaattataaa actgttttta tcactccctt
    22561 tgaccatgcc tatttctatc cagaagctac aagattaaat ctaaagctga tttttgatgc
    22621 agatagcggt cgtattttag gtggacaagc atttggagaa aaaggtgtcg ataaacggat
    22681 gggagaactt tctgtagcaa tcaccggaaa cttgacagtc tttgatttgc ctgatttgga
    22741 gttgccttac tctccaccgt attctactac ccgtgacccg ttgaatatag ctggttatgt
    22801 cgcaatcaat caaatgacga atatcgtaga aacgatcaaa gcaagtgata tacccgaaaa
    22861 cgatttgaaa gaagcgttct ttttagatat acgtgaacct aataaagcac cttctggcag
    22921 tatctcggca acaaaaaata ttccaatgaa tgagttacga gatcgaatca atgaaatccc
    22981 aaaagataaa aaagtttaca ttactttcag aagaggattg aatacttata cttctgcccg
    23041 aatcttggca ggtttgggta tcaaagcgat tttgattgaa gaataaaaaa acgaccgcgg
    23101 tgggtagccg cggtctagga gttattttta cttgaggagt aaaaatgaaa aagtatatag
    23161 ggttgttggt tggtatgtat atagaataga agataaatgt gaagaaattg tgcattattt
    23221 cttcacattt attaggttat agtttaagaa agtttaaaca ttgcgcaatt taattatttt
    23281 agctgtttct tttcataaaa aaacatgttg tgactctgag acaaatcata gtccatagtg
    23341 atgtctctag agtaactacg cctaatatta cgaggcatca tattaatcta tcagcactag
    23401 tacctaaact agcatccctt tcgaatattt tttcttatat aataaagttt gctttcactc
    23461 ctctaatctc accaaatcga ctggcttgcg ttgtgtgata ctaggaactg attggtttag
    23521 aacgactttt ctattgtaat catatttttc gccatcctcg ttgtcttcta atccttcaat
    23581 gaatgatccc taatgtgtgt attaaataat ttaatttttt tgttcctatt tatttaa
//
LOCUS       DACECJ010000039        22988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.039, whole genome shotgun sequence.
ACCESSION   DACECJ010000039 DACECJ010000000
VERSION     DACECJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.039"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IWO09_001582"
     CDS             complement(109..564)
                     /locus_tag="IWO09_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264173.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IWO09_001583"
     CDS             complement(718..1926)
                     /locus_tag="IWO09_001583"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0264174.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IWO09_001584"
     CDS             complement(2038..2370)
                     /locus_tag="IWO09_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0264175.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IWO09_001585"
     CDS             2518..3393
                     /locus_tag="IWO09_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0264176.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IWO09_001586"
     CDS             complement(3475..4056)
                     /locus_tag="IWO09_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0264177.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IWO09_001587"
     CDS             complement(4062..5321)
                     /locus_tag="IWO09_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0264178.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IWO09_001588"
     CDS             complement(5323..6369)
                     /locus_tag="IWO09_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264179.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IWO09_001589"
     CDS             complement(6604..6879)
                     /locus_tag="IWO09_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0264180.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IWO09_001590"
     CDS             complement(7122..8432)
                     /locus_tag="IWO09_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0264181.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWO09_001591"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWO09_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0264182.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IWO09_001592"
     CDS             complement(9978..10658)
                     /locus_tag="IWO09_001592"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0264183.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IWO09_001593"
     CDS             complement(10707..11342)
                     /locus_tag="IWO09_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0264184.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IWO09_001594"
     CDS             complement(11408..12811)
                     /locus_tag="IWO09_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0264185.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IWO09_001595"
     CDS             complement(12798..13829)
                     /locus_tag="IWO09_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264186.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IWO09_001596"
     CDS             13860..14081
                     /locus_tag="IWO09_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0264187.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IWO09_001597"
     CDS             complement(14246..14983)
                     /locus_tag="IWO09_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0264188.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IWO09_001598"
     CDS             complement(15136..16476)
                     /locus_tag="IWO09_001598"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0264189.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IWO09_001599"
     CDS             complement(16713..18236)
                     /locus_tag="IWO09_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0264190.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IWO09_001600"
     CDS             complement(18614..19225)
                     /locus_tag="IWO09_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0264191.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IWO09_001601"
     CDS             complement(19555..20271)
                     /locus_tag="IWO09_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0264192.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWO09_001602"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWO09_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0264193.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IWO09_001603"
     CDS             complement(20830..21627)
                     /locus_tag="IWO09_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0264194.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IWO09_001604"
     CDS             complement(21597..21968)
                     /locus_tag="IWO09_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0264195.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWO09_001605"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWO09_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0264196.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6445 a         4914 c         3899 g         7730 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctggtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctt
//
LOCUS       DACECJ010000040        22736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.040, whole genome shotgun sequence.
ACCESSION   DACECJ010000040 DACECJ010000000
VERSION     DACECJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.040"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(13..1275)
                     /locus_tag="IWO09_001606"
     CDS             complement(13..1275)
                     /locus_tag="IWO09_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0264197.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1268..2167)
                     /locus_tag="IWO09_001607"
     CDS             complement(1268..2167)
                     /locus_tag="IWO09_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264198.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2312..4090)
                     /gene="uvrC"
                     /locus_tag="IWO09_001608"
     CDS             complement(2312..4090)
                     /gene="uvrC"
                     /locus_tag="IWO09_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0264199.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4272..4586)
                     /gene="trxA"
                     /locus_tag="IWO09_001609"
     CDS             complement(4272..4586)
                     /gene="trxA"
                     /locus_tag="IWO09_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0264200.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4673..7033)
                     /locus_tag="IWO09_001610"
     CDS             complement(4673..7033)
                     /locus_tag="IWO09_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0264201.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7165..7695)
                     /locus_tag="IWO09_001611"
     CDS             complement(7165..7695)
                     /locus_tag="IWO09_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ0264202.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            complement(7753..8190)
                     /gene="zapA"
                     /locus_tag="IWO09_001612"
     CDS             complement(7753..8190)
                     /gene="zapA"
                     /locus_tag="IWO09_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0264203.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8392..9309
                     /locus_tag="IWO09_001613"
     CDS             8392..9309
                     /locus_tag="IWO09_001613"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0264204.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9565..10476)
                     /locus_tag="IWO09_001614"
     CDS             complement(9565..10476)
                     /locus_tag="IWO09_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0264205.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10879..11457)
                     /locus_tag="IWO09_001615"
     CDS             complement(10879..11457)
                     /locus_tag="IWO09_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0264206.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11477..12145)
                     /locus_tag="IWO09_001616"
     CDS             complement(11477..12145)
                     /locus_tag="IWO09_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264207.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12160..13230)
                     /locus_tag="IWO09_001617"
     CDS             complement(12160..13230)
                     /locus_tag="IWO09_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0264208.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13401..14765)
                     /locus_tag="IWO09_001618"
     CDS             complement(13401..14765)
                     /locus_tag="IWO09_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0264209.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14762..16321)
                     /locus_tag="IWO09_001619"
     CDS             complement(14762..16321)
                     /locus_tag="IWO09_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0264210.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16377..18857)
                     /locus_tag="IWO09_001620"
     CDS             complement(16377..18857)
                     /locus_tag="IWO09_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264211.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18841..19176)
                     /locus_tag="IWO09_001621"
     CDS             complement(18841..19176)
                     /locus_tag="IWO09_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264212.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19190..20224)
                     /locus_tag="IWO09_001622"
     CDS             complement(19190..20224)
                     /locus_tag="IWO09_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0264213.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20314..20994)
                     /locus_tag="IWO09_001623"
     CDS             complement(20314..20994)
                     /locus_tag="IWO09_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0264214.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21278..22381)
                     /locus_tag="IWO09_001624"
     CDS             complement(21278..22381)
                     /locus_tag="IWO09_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0264215.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         6488 a         4779 c         3865 g         7604 t
ORIGIN      
        1 tttttctata ttttatgctt tttttcgttc atttctcata attgaaatcg attgtttcaa
       61 tgaactccat aatccgcctt cgctatacac gagtacattc gatttgtaca tgtttgcaga
      121 caagatcatc aataagaatg ttacggctac aagtaccaac aaggaaatca atgctccacc
      181 cataccgact gtctcatttg ctaagcgaac cggcataata tatgaagata agaatggaat
      241 atacgaagtg atccgaataa cgatattgtt tggatctgaa gctcctagta tcaatccaag
      301 catatatccg cctagagata gataagtcac tggcaaaatt gcttttgctg tatcttctgc
      361 tttgttgacc aaagagccac ataaagctgc taaaacagaa aaaatcaaaa tgccaaagat
      421 catgaataga agagaatacc acaagaatgg tccaatcaag ttatccaatg agataccgtc
      481 taatatattt ttgaccattt ccatatcttt gaattgactg tatcctatac caaaaataat
      541 accgtataat gccatttgcg ttaatgcaac aagtaaaaca cctgttagtt ttccgtaaaa
      601 atgcttttgt gcagtcgtac tggacaggat cacttccatg atccgcgtgc ctttttcaga
      661 agcgatctct tgtgcaatga tctgcgcata cgtcaaaata ataatgaaca agacaatcgt
      721 tgctacataa cttacgacat attggactgt cgagttatct tctccaactg tcatttttcc
      781 ttttgaatca aaattgactt tttgtcgagt caaacttgct tgttggctca agctagctac
      841 ttcttctggt gaaatgccta attgacttgc tcgaagcatc gattgcaagc cagtcagttg
      901 ttgttgtatc agtagttgtg tactttgtcc taatgaattt tcgctataaa gcatcccatt
      961 tatcttatca ttctcagttg agactactaa atacgcatcg attttttctt ccgaaagctc
     1021 tttttgtgct ttttcttcag aatcaactgc tttgaactca aaatcatctg ttttgacttg
     1081 gctcatttgc tgactgattt gctcattatc agccaatacg ccgattttat tgacatcact
     1141 attttgttga gcaaagtttc ctgctacata aatgataccc agcatgataa aagggactaa
     1201 tatcatgatc aagaaagaaa tcgacttaac attttttaaa tagacatctt tggtaataat
     1261 ccaaaactta cgcatcgatt tcacctgctt tcaatttgaa aatctcttcc aaagttggcg
     1321 gttgctggtt aaacatagga atgtagccga actgagtcgc acgagtaaag atttctttcc
     1381 cagcttcagg atcttctaag gtaatctcta acgtattgtc ttcatgaagg atcacttttt
     1441 tcacgccttc gatttcttct acttcctgtt ggcttaaacc agattccaag aataacttcg
     1501 tacggccaaa tgattcacga atctcatgta cttttccatt caaaaccatt tttccgtttc
     1561 gcaacatgat caaatgatcg cagatttttt ctacattatc catattgtgg ctagaaaaaa
     1621 tgacacatga accatgtttt ttcaattcca atattccatc ttttagcaat tcagcattta
     1681 ccggatctaa cccgctgaat ggctcatcca aaatgatcaa tttaggttca tggattaacg
     1741 tggcgatcag ctggactttt tgctgatttc ctttagacaa agattttact ttatccgtct
     1801 ttttcccttt tacctgaaat ttttccatcc aaaaatcgat ttttggttca atctcttttc
     1861 ttgatttccc tctaagttct gcaaaataaa gcagttggtc ttgaatcgtt actttcggat
     1921 aaagtccccg ttcctccgga agataaccga tgacatcata atctttctcc ccaagatgat
     1981 ggccattcca taacacttct ccttggtctt gtgtcaaaaa atccaagatc aaacgaaaag
     2041 tcgttgtctt cccagccccg ttttggccaa ttagccccat gatttttcca tcggggattt
     2101 ggaaagagac atgatcgaca gctgtgtagt tcccaaaagt tttaacaaga tccttgactt
     2161 ctaacatttc ttcactcctt cttcaagaaa tcacatatat ccttagacac cagacgtaag
     2221 cgatttatta tttaccaaca aaacgtaaac gctctttttg ttgtacataa aatgatagga
     2281 cactttactt tctttgtaaa gtgccctttc ctcattcctg atgtaattta tcaaatacgt
     2341 tttgtgcgac atttttcggc agtccgattt cttgtaattc ttctgtcgtg gctgctgtga
     2401 tatttttcaa cgatttgaac tctttaagca gcattttttt tcgttttggt cctaaccctt
     2461 caatttcatc taatcgggaa gcaaaactgt ttttactgcg tagctgccga tggaaagtga
     2521 ttgcgaatcg atggacttca tcttggattc gctggagtaa gaaaaactct ggtgaatttc
     2581 gctcaagcgg tactacagcc aaatctatac cgaatagcaa ctcatttgtt ttatgtttat
     2641 cgttttttgc catgccagca ataggaatat cgattcctaa ttgattttct aagacatctt
     2701 ttgccacatc tacttgtcct tttcctccat cgatcaagat caaatcaggt aaaggcaacc
     2761 cttcgcgaat cactcgtgaa tatctgcgat agatgacttc tctcattgat gcataatcat
     2821 ccggtccgtt gactgttttt attttatatt tccgatattc ttttcttgcc ggttttccat
     2881 caatgaatac gaccatcgca gccacaggat tcgtccccat gatattagag ttatcaaaag
     2941 actcgatacg tattggggtc gggatattca tcgcgtttcc taaacgttca actgctccga
     3001 tcgtccgttc ctgttttctg gcaatcagat caaaccgttc attcaaagca actgaggcat
     3061 tcttattcgc caattcaact aattttttct tttctcctcg ttttggttgc agcactttcg
     3121 tagctaacaa agcttctaca ctgggcttgt cgatcgtatc aggaatcaag acttctttag
     3181 gaatgaaatg ctcattttcc tgataaaact gaccgaggta agtcaagaaa tcttcttctg
     3241 cttcgttata gaaaggaaac attgaaacat ctcgttcaat tagctttcct tgtcttacga
     3301 aaaacacttg gacacacatc catcctttgt caatcgcata accaaataca tctcgatcca
     3361 gtaaatcagt atttgtcatt ttttggcgag tcatgattgt ctcaatggca cggatctggt
     3421 ctcggtattc tgcagctttt tcaaactcca tgttttcagc agctttttcc attttctgat
     3481 ggatctctga ctcgattttt tcgtgtcctc cgtttaaaaa ctttttcact tcatcaacga
     3541 ttttcgtata gactttcgga tctacgtcga aataataagg acacagacat tgtcccaaat
     3601 gatagtaaag acaaggctcc ttcgtctgac taggtttgca ttttctcaga ggaaataatc
     3661 gatcgaggat cttctttgtc tcattcgccg caccgacatc gggatatggt ccaaaataca
     3721 gtgctttgtc ttttagtact ttacgagtaa tcaccaaacg gggatatttt tcattcgtta
     3781 tcttaaggaa aggataggtt ttatcatcct ttaacataat gttgtattta ggatcatttt
     3841 tctttatcag attgatctct aagagcaatg cttcgatatt cgattctgta acgatgtatt
     3901 caaagtcttc gatttcacta acaagccgtt ccgttttcgt attatggctg ccagtaaaat
     3961 atgagcggac acgatttttt aatattttgg ctttgccgac ataaatgatc gttccatttt
     4021 tatctttcat caagtaacag ccgggctgat caggaagtaa agctaatttg tttttgattc
     4081 gttcgttcat ctggtactcc ccttcatatt caattaaagt tattatagca tgtctgtcaa
     4141 aaaaacaatc cagcctgaca acgtaattat actcaaaaaa tattaatttg aataacctta
     4201 actcaaccgc aaaaaaaccg aggacggaac tggattcagt cccaccctca gcttattttt
     4261 gctgtaagtt tttataaata ttgatcgatc atttgacgta gttgttcttt tgaatgaaca
     4321 ccaacagctt tttctactac ttgaccatct tttttcaaca ttaatgttgg aatgctcatg
     4381 ataccgaatt gttgaggagt ttctggattt tcatccacat ccattttagc aattctgaat
     4441 tcttcttcat catattcttg ttccaactga tccaaaatcg gtgcttgcat acgacatgga
     4501 ccacaccaag ttgcccaaaa atcaatcaat acaagtcctt tatctgtttc ttcattaaat
     4561 gtcttatctg taattacttg tgacatatca aagacctcca tttttaaaat ctatgattag
     4621 tatagcattt ctatacttgg atagcgaacc atctgcttac ctttagtaag ctttacttaa
     4681 atttaacaat tgtcgcacca tttccgcctt ggtttgccgg tgcaaactca aagcttttga
     4741 cgctccgatg gtttttaaga taatcagtaa ttcccttgcg aagtgcgcct gttccttttc
     4801 cgtggacaat agtcacttga ggataaccag ctagaatggc agaatccaaa tattgatcta
     4861 cctcacttag cgcttcttcg tatcgttttc ctcgaaggtc caattgattc ggcacgtgac
     4921 tactagattc agatcgtaca gcatggataa ctcgttgtgt cggctctttt tgaggagcaa
     4981 caggcgtcag ctcgtcttcg gacacattca tttttaatat accgatttct acttgccact
     5041 gattcccgtt tttacgtaaa agcgtccctc tttggccgta agtcgtaacc aacacttcgt
     5101 ctcccggttt caacgttttt tgttctttcg ccttcttcaa tactttattt ttcttcaaag
     5161 tttcttcttg atgtagattt gctaattggg tctttgcatc gatcaactga tgctctttca
     5221 catttccttg cccaatctgc tgttgcattt tacgaatatc agcaatgatt ttttctgctt
     5281 tttcttccgc ctctgacacg acttcgtttg cttttttctt tgctttttcc atttcttttt
     5341 cacgttcttc aaagaaatag ctgtaagctt ctttcaaatc atcgtgaagt tcttgtgcct
     5401 cagaaacaaa atgacgcatt tccagatatt cggtctctgc cattttccgg cgattctcta
     5461 aatctgtgat catttcgttt aaatcttggc tctcatcatt catcaactgc ttcgcttcat
     5521 caataacttc tgtatcaaga cctaatcggc tagagatttc aaacgcattg cttcgtccag
     5581 gaacgccaat cagcaaacga tacgttggac ttagtgtatc cacatcgaac tccatgctgg
     5641 cattaatcgt atttgcccga ttgtatccat aaactttcaa ttcaggataa tgcgttgttg
     5701 ccatcacata agcagacttt ttaccaagat catctaagat tgcgattgct agagcagcac
     5761 cttcttgcgg atctgttcct gcaccgagct cgtcaaataa taccagactt ttttcattga
     5821 cttttgataa aatatctaca gtgttcgtca tatgagaaga aaaggtcgat agactttgct
     5881 caatcgactg ttcgtctcct atatccgcaa atacttcctc aaaaataccg atttgacttt
     5941 cttcacctgc aggaatcggt aatcctgctt gccccatcag ttgtaacaga cctaaagtct
     6001 tcaacgtgat cgttttccca cctgtgttag gtcctgtaat gacgatggct tgatagtctt
     6061 ttccgattgt gatgtcatta gaaactactt tttcctgatc gatcagtgga tgtcttgctt
     6121 gtttcaagac gacatgattg tcttcactga ttcccggcac gatggctttt acttctttac
     6181 caaatcgtgc ttttgcattc atcagatcca ttttaccaat cacataagca ttgtggatga
     6241 tttcttgacg atggggaaca agttcagcag aaagttcact taaaatacgt tgaatctcat
     6301 ttcgttcagc gatttggtat tgacgcaaac gattattcaa atccaccact tgttttggtt
     6361 caataaataa agtctgtcct gaagcacttt gatcgtggac tacaccgcca aatacaccgc
     6421 ggtattcttg tttgactggg ataacgtagc ggtcatttcg catggtgatg atcgcatcac
     6481 ttaaatactt tgcatttttc ccacggacga tcccatctaa ctgttcacga acagcttgtt
     6541 cacttcgccg aatgttctgt cttatgctct ttaactcagg agaagcgtca tcagtcacgc
     6601 gtccgtcttc atcgattgct tcctttaaac ggcggctcaa aacagggatc gtgactaact
     6661 gatctgccca catatacaaa cgtgcaaatt cgatttcact gtcagaaaga tcatcaatga
     6721 aacgattcaa ttcgcttgtc gtagacaata ctctggaaac ttgtgcaagc tccactccat
     6781 tcaagtctgc tccgatctcg atccgtttca tatggggacg gatattttca agtttaggta
     6841 tagggatccc gcctcgcaga cgttgcactt tcaatccatc ctcagtttct tgaagccagt
     6901 ttgtgatcgt ttgtttctca ctcactggta ccaattctgc cagttcttct tttccttgtg
     6961 cagtcactac aaattgtctg accatctgtt tgactttttc aaattccaat gtttcaagga
     7021 ttcggttatt cattttatca ccttatcttt cactatgaaa aaagagctgt gccggacata
     7081 cgctacgcag ccttcttaaa taaactactc tgtacaaaaa acgaaatatt ttccagcact
     7141 attttcctta attcaaaata ttcgttacca aagctcatac atcttgttgg taagaaatgg
     7201 cgttctctca acgataaaac gagcgaaact atgctcgccg aattgatttt ggaccacatc
     7261 taatggaaca aaggcaagta actgcaaaat caaaaatata gtaacgtaag ccatgattag
     7321 tgacaggatg cctcctgcaa tccagtctac ctgtctcaag atcggcacat acgtcaatcc
     7381 atggacaaaa acccccacaa atcgggtaag cagccagcca attagcaaaa taatcagaaa
     7441 agcagttcct gcataaaaag cttcatctag gtgaaaagca aatacctgat caaaaaatgc
     7501 taactttgat gttggggtga cagctgggta tggaatgtat aattccaaat gacttgctaa
     7561 tggtttgtaa aaatgctggg cagcaataaa tgacaataca tatccaatcg cgaatatgat
     7621 ttgcaaagca aacccgcgac gtgctccaga aaaaaacgca attagcaata aaaataaaat
     7681 cagcaaactc agcatgctac gttcctttct cttctaactt ttttaaattt ttcttatttt
     7741 gataaaagga tcttaattag aagatttttg tttaatttgt ccttttcgtt tctcgtttaa
     7801 aatctgttgt gcttccacat gatttttaat ggtatgatct gcttgaccat tttctttcaa
     7861 tacttcccga gcttcgttct cgattgcttc aatgcgtttc acacgatttt caagctctgt
     7921 aaatttgatc atttttttct ttaattctgc tgtttcttct tccagctcca agattctttc
     7981 ttgtttcttc aattgatccg aaagcgcatt gacagccatc aagattgctg cttgttcatt
     8041 atccatttgt ggtgataatt gtttcaattc tgctaactgt tcattgatca gcttcgttac
     8101 gatatccata tgatgcttgg tttcccgtcc gataatagta tatgtttggt tggcaatcac
     8161 tgctttgtag cgtgtttttt cgtgcgccat acgaatcatt tcctcctatt tgatgaaagt
     8221 tttatttagt gtaccatgaa gttggttctt tcaccgttct ccacaaatat ttttcacata
     8281 cttaactatt atatgataaa ctttaaaaaa atgctatgtc tcgcaatctc ttagaaaacg
     8341 aaaagtcgtg agttgcggta tagttttatg tataaaaacg aggagaattt tatgagcaac
     8401 gtcattatca aagcaacaaa cgaccaactt agtcaaatga aagcatacta tcgctcttat
     8461 ttgttaaata aacagattcc ttatactagc tttgctgcaa aaaaagacgg gacgaccatt
     8521 accgcttata catccggaaa agtgatgttt caaggtacga atgcagcaat agaagctgat
     8581 aaatgggggg aggcacagga taaacaagcc aagccagcaa aaacagcctc ttctttgcca
     8641 aaaaatatca gtcaattatc cgttttaggt agtgatgagg tcggaaatgg cagctatttt
     8701 ggtccgctaa cagtatgtgc tgcttatgtg aaacgtgatc agcttgcaga attacgtaaa
     8761 cttggtgtta gagattcaaa agagttaaaa gatagccaaa tcatccagct tgctgacatc
     8821 ttaaaaaaaa ccatccccta taaactgctt gtcttatctc cagaaaaata caatgagatc
     8881 caaccaaaat acaatgccgt tcggatgaaa gtcgctttgc acaatcaagc gatccatttg
     8941 ctgctaaaaa aaatcgagcc agaaaaacca gaagcgattt tgatcgatca gtttacgccg
     9001 gaagcaaatt ttaaaaaata tgcgcgttta gaaaaaaatc aattacaaga aaaactgtac
     9061 ttcgttacta aaggagaaca atatcattta gctgttgctg ctgcttctat catcagtcgt
     9121 gccagctttt tggaagagtt acacaaagca tcagctgaag ctggaatcac ccttccatca
     9181 ggagctggaa cgaaatctga ccaagtagcg gcaaaactat tggagaaagg cggcatgcct
     9241 atgcttgaaa aatatgccaa acttcatttt gccaatacag aaaaagcttt gaagctgcta
     9301 aaaaaataat ggaactgtga caaaagtcat gtcacagtcc cttgttccga ataaacggtg
     9361 ttctcatgcc aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt
     9421 ttgaaattgc tccttgtgac gcggagttaa acggcaggct cacaacctct tccttaaacg
     9481 gtgttcaaaa gcttgcatct gcgataataa gcccggccaa acgaaaaata tgaggagcta
     9541 tttttgtttg gctgggctta tttattactg aaaataagtg gtcaatgctt gtgtcaaact
     9601 tgcagcaatc tgctgctgat aactgtctgt tactaattcc tttaaatcac tatcattatt
     9661 catataaccc atctcgatta aaatagaagg ttgaagattt tctcgaagaa cttgatagtt
     9721 cccagtggag attcctcttg aatccagagg cagatcagtg aaggagtcag caattgtctg
     9781 tgctaatgtt tcctcttgtc catagtaata atatgtcgtg attccagttg cttcattcgc
     9841 atattccgta gagtctgcgt gtaaactgat aaagacatct gccttttttt cttcactgta
     9901 gtcgcatata tctccaagct gtactaattc atctgtagta tgggtcaaat aaaccgttgc
     9961 tccagcatcc tctagcgcct tttttacttt tatggctgta cttaatgtta tatctttttc
    10021 ttctgtttgt tcatcattgc ttaatgcccc agtatcattt ccgccatgcc caggatctaa
    10081 tacaatcact gcatcagata gctcttccgg ttttgtttgt atattctttg tgactttggt
    10141 tccatcttcg agggctaaat atccagtata gccatcattt gtcttgatct gataagcatt
    10201 ttttgtttca gaaagaaact ccgccagctc tccgccgttt atagttgcaa gtggggtagt
    10261 tagcgagtca tctgtatata gtgtacggtc attttttccg attttgatta atgtctcttg
    10321 ttctgtatca tcaaatgatt cctttgtctt tgtaaccgta ctgttcgtac tgctgaccgt
    10381 ttgtccaact atcttttttt gaccaaaaac atctatttga aaacagataa taatgataat
    10441 gattgcaaat acaaaagctc caattatttt tttcattctt gactctcacc tcgtagatcc
    10501 agactaaggg tcaaaactta aaatcagctt aagtacgcaa aaaaggggct gtaaaagata
    10561 taaccgtaga aaataacatt actgctaaaa tgattttttt catttttggc atatcctgaa
    10621 atctcctttt gactattcgt ggtgattcat ctacccaata aatagtttcc ttcattctac
    10681 aaacaattaa tttcttcgtt attaaattaa aacacttcct ggaacaccga cataataaat
    10741 aaaaatatac cctgataatc atgagagaat aacaaatttg tatttatgaa aaactaccta
    10801 tataaaaaaa acaagatata acgatcttcg tcatatcttg cttcttcacc taactatttt
    10861 tctccctata taacagcttt acgcttctcc taatacacca ttcaaatata aatcgatcaa
    10921 ttgcgccaca tttagcggtt cttccacagg tcttttcgac cgagtgatgt aaggtgctaa
    10981 cactaagaaa aaatgccttg ctgcgatttc ttttgaaatc ccatttctta gcacagattg
    11041 gtttgctgaa aagatttctt caaaaggttc acgataattt ttcttccata gaaagaaaag
    11101 atgatctctt gtttcctttg aaagattgct ttcaagatcg tgaagcatca gaaacaaatc
    11161 gaccagatgt gtctcaatca aaaactgcgc catttttgtc aaggttactt gaaatcccgt
    11221 ttcgcttgtt tgaacgattt ctctcaaagc ttttccaaca tcttttaagt ggttttctaa
    11281 aactgccgta tatagttttt ctttatcacc gaaataatga tagagattgg gttgggtgat
    11341 cccaactttt tgtgcgatct ctcttgttga agtgccgaga tatcccttgg acataaacaa
    11401 gtcagtcgct tctttcaaaa ttttttgctg tgtatctttg ttgtatcgat ggaccatttt
    11461 tccacctgct ttttggttat tctttttctt tcaatacacc atcttccatc ataaatattc
    11521 tatcgcattt gtcaatcagc cgtgtatcgt gagtaaccat gatcgttgct ttatttttgt
    11581 cttttgattc ttttgccaag atatcgacta cttctcgtgc cttttctgaa tccaaactag
    11641 cagttggttc atctgctaga ataatggacg gatcattgta caatgcacga gcgattgcga
    11701 ccctttgccg ttctccacca gatagttctt ctggatactt gttgatcaac ttatccacgc
    11761 caagctgctc aaataattct tttgcttctt tttgacgatg ttcatgtttg atcttatcta
    11821 ctaataataa ctgctttttt actgtcagaa aaggaaccag attggaagct tgcaaaataa
    11881 agccaatttc tttaaaacgt agtttaacgc gttcactttc tttttgatta ctgaattctt
    11941 gatcattgat aaatacagag ccactgctag gcgtctgcaa tccgcctgcc aaagtcaaaa
    12001 aagtactttt acctgaacca gaaggaccga tgattgcaac aaattcccct ttatcgatac
    12061 tcatggttgt ttctttcaat gctttaacta cttggtcgcc atcttggaaa cttttttcta
    12121 cttctttaaa ttctattgct ttcatgctct tcctccttct tatccaattg ccttcaacgg
    12181 atcgattttt acgattgtcc gaacagaaaa tagtgcgcct aacacagcta ctaccagcat
    12241 caacaagcta atgacgccaa aaaacagcca attgctttgg aatggaaccg catcaggtag
    12301 aacaagggat gttcccactg ttcctaatag accaagaaga ataccaacaa atgataaaat
    12361 gaatgtttgt gccactacag aacgcgcaat aaatcctcct gtgatccctt gtgctttcat
    12421 tacaccaaag atattaatct tttgcatcgt cagtacataa atgaaaatcc cgatcacaat
    12481 agccgcaata acgatcaaaa atccgatcat aaatccaaaa gtcaatactt gcgcattata
    12541 tccaggtaat tcattgatga atttagaaat tttgatctgt tctaaatctt caggtaaatt
    12601 actgatttta cctctgacaa ctattgcgtt aatccgtgca ttttcacttg tatcttcttt
    12661 ttcgaagcgg atctcttggt aagcattgat ggttgtatag agaactggtg agacattgaa
    12721 tttggcatga tccgtaaagc cagttagttt aaacgtcttg tcactaccag ataacttaac
    12781 tgtatctcct acggctagac catattcttc tttcaaacta atatcgccta ctgcctcatc
    12841 atcgttatca aaagcttttc cttcaacaag attgggcatg atgaattgat ttttatcgat
    12901 accgaagaag ctaacattga tttttccttc ttctgtactg tctttactag tcacgactcc
    12961 aggtgtctgg gcaagatacg ccactttccc accttcgact tcttctgcta tcttcttagt
    13021 aatcatggac atccctagat ttgaatttgc atctttcgaa agcacaatcg aatctgcctc
    13081 ccacttgtcg actgctgttc gattatcttg agctaagcca taagctaacc cagtcaaaaa
    13141 aaataccagg taggcgatca aaaacatcac ccctgcaact aacgcataac gtaactttga
    13201 atgcataatt tcatttaatg ctaaaaacat ttaactcctc ctcctgaaat aatcatttta
    13261 tacaacaatt ttatcacttg ataaattttt ggcgaatatt atgctcaaaa aattcttccg
    13321 ctatttcatt atatgaattt tttattctcg attgattaat atttattata tccccacaca
    13381 ataaaagacc gtgatatttc tcacagtctt ttatctactc tattgctatt tgctctctta
    13441 gctagtactt taaaagctaa ctcattgagg tagtgaaaac gagaggtgtg ttcagacaga
    13501 aatccaaaat cctcaactgc tagctgtgga atcgtagttt tgtgtttcac gatatccgca
    13561 agcaggttca aatctgataa taagaattct tggcttatca gctgtccgcc aattatttct
    13621 aatgtatccg gatggtagat cagctttgca tttactgttt tgttttcttc atcgtattgt
    13681 ggaaatagat caaattctcc tgaacacgaa ccggtatttt gttccaagaa tggcgcttca
    13741 tcttctgtca gccctacagt tcctaaaaag tagtctataa tgactgctgc tgttatcttt
    13801 tgtctgaggt cgtaagcggt tggctgacca ctcaaagtta gcgctactgc tcttgccata
    13861 tgtacagctc gagtaactaa aggtaaaaag agatgccgtt tatttttgtc aaaactgact
    13921 gggacgatat ctccaatcgc atagatgtcc ggatcacttg tctgcagata ttcattgact
    13981 atcacagtct catcctcata caaagccact ttttctttta acaatgtcgt attaggacga
    14041 gtttgggcag aaaaaatgac catatctgcc tttattgctt gagatagaag ttgtacggat
    14101 actactttct cttctgattt tttccaatcg atcaagaatt catttggata aaaattgatt
    14161 tgcttagttt gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa atatcttgat
    14221 aaaacattcg gcatataatc aactagatga ataatgtatc ctttatttct caaagcatct
    14281 gctaattcca atccaatata accagcaccg aagatcacta cttcttttgc ttcttttaaa
    14341 gcggcaagta cttgcttaga ttgcactaga tttttatagg tgtagacatt ttcagcttca
    14401 atttgtttat acaaggtgac aggagaggaa cctgttgcta cgattaattt atcataggtt
    14461 atggtctcgt tttttccttc tttggatgcg aggatgagct gatgtttgtc tggatcaagt
    14521 tcagttactc tccattcggt gatcagtttt gccccacttt cttgaagagt ccgcatgtta
    14581 tgggcaactt cagctaaact acgtatcttt ccttttaaat aaaggttgat tccatttgaa
    14641 acatagccta gtccatcttt atgatcttca tcgatcagta aaacttctgc tttcggttga
    14701 agttttctta gatttagggc agctgtgatg cctgcatggg aagcaccaat gatcacgatt
    14761 ttcatgtttt attcatcctt aaattcaatt tttctaaata acattctttt aaaaattggt
    14821 tcaaataggg gcaatcataa ttatattcgt tgctagtgaa tacaaaaact ttagcggact
    14881 tctgtgacgg gtactctttc aattggctag tcaaaatgat atcatactgc ttttctttct
    14941 cgtattcgac taaatcatat ttgatcgtat tcagcaattg ttcctggatg atttctttca
    15001 cgatatccgc cattagcctt tcataaggaa gatcataagc aatttgcaga cgtaattcta
    15061 actgcttgat cagcgtccgc aaaatagatg catagcgagc aaataattca aagcgggcat
    15121 tttcctcgtc tacttcagta aaatcaacaa tagcaaatgt ttgtctgacc aatctttcac
    15181 ataaaaccag gaattgttta tcttctactt cagttaaccg ctgttttaat ccttcttggc
    15241 caaaaatatt catccatcct ctaaaatagg caaaatgagc gtgattttgc atcactgtca
    15301 acgcaatctt ttgcgaaatc tcttctggaa aaacataaaa agagaaatat tcttctatcg
    15361 attgtttaaa cgttcgattc aattgagcta ctacgtcgtc tttgtatttt tcgcttatca
    15421 aaaactcctg gacatgcgag ttatgtgtat ctaatgtaaa gttactgatc atgaaaagat
    15481 aaaaaaccaa cgtttcattt tgattccaag gaaaggcata ttgaaagaag taacgggtca
    15541 aaaatgtatg gaggtccaat aaaaattgtg tcgtttgaaa caatccaaca ggaagattgc
    15601 tataatctgg tttggatttt ttcgtacagc gcattcgctt cttcataatg gaaagccata
    15661 tttcaaattt caatcgtcct tcttctgtaa tagtaaaact aaactctttt tgcagcagat
    15721 ctaaaaaagt gtttcctgat aaatcactgt attctttttt gttttcaaga taaggacgtc
    15781 caaaccaaaa taattggaaa taaaaataac aaatttgact ttctggaccg gtcagccgcc
    15841 cgctttttat tttcagatga aactcattta aagaatcatt taattccttc actctgcgag
    15901 acaaagtagc cgaactgata ttaagttcca tgacaagttt ctgccgatca aatgagccat
    15961 tttccaacag ataaagaagt atctgatatt ttgtagaata gcgcaagtag caatgataat
    16021 aaacatcgac tgctgctata tttccatgtc tgattaataa aatattccct tcttggataa
    16081 caatttccaa ttgtttgata ttttccaagt tagcttgtaa ataaaacaga tcttctttca
    16141 aggtagcccg atcaactgat aagcgggtaa gtacgttatt aacggaaatc tctccttttt
    16201 cctctaataa aagttggaaa agtttgattt gccgttgttc atttttttca agcaaactga
    16261 aaagttccaa gtccactcct ccaagcattt ctgattttaa cagcagaagt ttccttctca
    16321 tccttgaaaa agaggatctg tgacagttat cacagatcca tacaaattct aatcacttag
    16381 ctggttggcc cagctgtcag ttcccatgtg accatgcctt gatacgttgc gccaggttga
    16441 gcattcgtac tgctatttac ctctaagagt aaaccatcgg tatcgggaaa gtcaacgaga
    16501 acctctcctt tagctgttcc atcactctca taaatcaacg tgttttgacc ttgctgaatc
    16561 aattgttttg atccagaatg attgaagtat aattttgttc cgcttaattc ttgaccttga
    16621 tcattcttaa ataaagaagt caatgtccct gtcagttgcc aaggagacgt gcttggaagc
    16681 cgagtatccc gcacatcaaa agaaaaatcg ccttttgctt cataataact tgtcttcaac
    16741 ggtttgggga gatcgttacc aaatgtaaaa tcatctggta cattaactaa tgttaattct
    16801 tgtttgggga caagaattgt tttagcagat gtttggacaa ctgggttagg aagcttagca
    16861 ccatctgcta atgtaatatt gctggaaagt acagctgcct ttgtcagtat gctgacggat
    16921 tgtacagaag tatctacagt atttaattga tagttgtaag taagatgaat ggtatctcct
    16981 attttgctca atagatctgc tgggacagaa actccttgtt gaatgccggt atctgcgata
    17041 gttgtaccat ttaattttaa ggaaccattg atataagtag tattttcagg aagtgagatc
    17101 gtgatttttt tgtcggttat cgcatcccca gattttgtta acgtgaatgt cacattttct
    17161 tgaatcgtat ctcctgatag agcagacagg ggatctcctg tctcaggttg tctgctaagg
    17221 ttttttagct gactatctgc actcacagat ggaaaattag ccactgttat ttgaggaaga
    17281 gtggtgattc cagcttgttc agactgccat ttgtacgcaa aagacggata catgttagta
    17341 agtacatcgt ccaccaagcg ataatcaaaa taatattgcc ctgaagccaa tgcaggtagt
    17401 gtagaagtag ctgtatttac acccccgtta tttgattgga tcgtttggaa aagttgatct
    17461 gtccatcctg tcgctaattt ttgggttcct ttcgaataaa gcctgtaatg gatcgaaccc
    17521 ttttttagtt cagttgctga ccaattggcc gtgacttggt acgatgcaga agaaaggagc
    17581 tttccccact gactatcggc tgttaagttt gttggtaaaa ccgttccgtc tagcttgtac
    17641 tgctccaata cctttaaagc aacaggagaa agcaatccgc cttgcgcatc tttcccactg
    17701 tacacgatat tggctgtctg attcaatcca cttgtgcttg ctggagtatg agtcagtatt
    17761 gctccttgtg taacgttttt tcctgtaaaa tagttgacta cgcgatcaaa gcgactaaat
    17821 ggcaactgat caaaacgtcc ttcagtcagc cccatcgtca atgtccgact attggtagag
    17881 cctggaaggt tctggttatt tccatccacg tattctgtgg tatcagcaaa tgtattatat
    17941 tttcctgttg aatcacttgt tgagatgtaa ctattccacg tatttatctc tgcacttgca
    18001 gaaaatgcat atgctcctgg attcaagttt ccgccagtta atttaggata aaatctacct
    18061 gcagctggag ctaaaatcga attgaacata cgatcttgga taatattatt cgaatcataa
    18121 aaatcaacag aaaaaacggc ctttttagac atagaaggtt gtaatttacc cttggtcaaa
    18181 attttgtttg gactcgtatc atatttatct ccgttttctg tcatcttcgc gacattagct
    18241 gtaacatcaa attgaagcgg gatatcgtcc acgttgaatt cgcctcgcgt aataatagca
    18301 ataatatctg ccaatagagt atttgtatcc cacgacccca tcccaaaacg caaggtatgg
    18361 cttcctgctg ataataaact accgctagta accttgatgg gggcaccggc aatatacaag
    18421 taggcgttgt tccagtcaat tgcttttaat acatcactgc ttgatacatg ttccggcaaa
    18481 ttgatgatcg ccacaaatgc aaatccttgt gcagtgatat tcaccaaatc aagtacattt
    18541 gttagtttca catggctttc ccaatgaaaa cggaccgtgc cacctttcca aggatcattt
    18601 gaaccatcgt tcaaataagt agcagtcaat ttaggcaatt ggttagcccc agttagttgg
    18661 acaagcttcc cgccattgac tgcactacgt ccaccatttg gaggtacgat attttcatac
    18721 gtgtatggat cataagaaat ggcaggaggc gttgctccaa cagcttctgc cgaaggtgaa
    18781 aagataaata tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa aaatgaaaat
    18841 ttattttgta gcaacatgtt catcctcctt ttcctttctt ctccataaca acaaccacgc
    18901 aagaagccat aattctatac caatgatcaa ataaagaaaa cggatctttt cttcattcgt
    18961 ctccgggaat acgccatgtt gattagattc tttataaatc ggtagctgtg tctgctgttt
    19021 tgatgcgcca ttgactgaac cacttggatg cggaactgga cttagatcat aaaatgtaat
    19081 cgtacctgaa cttttggtat ctgcttggac tggctcttgc cagctgaaga acagcaaact
    19141 tgcaataatc cctcccaata agatcgatgt tttcaacgaa atcacctcat catgttgttt
    19201 ctcctttttt ctttttcttg tggacccaca aaaacagcaa aatcaaccat aacactagaa
    19261 ctgcagcaac caatcctgca taaagccatg ggaaagattg ttcttgtact gcaaaaacgg
    19321 aattttcatt tactttttgt gctttttctt ttttgatcgt aaagttttgt gtaaatcgcc
    19381 attcattact gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg gcttttgctt
    19441 tatcaccagc taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact aaaaattcat
    19501 tagacgtatt ttcaatataa agctgatttt cataaaagat cttgctcgta aatatcaaat
    19561 tgctgataat cctaggctga ggattttgga aacgaagctg aacagttggc tttgcgttga
    19621 cctcgtccaa atctcctgat aaaagtttca gatccggttc tatcgtctct tgactttctt
    19681 gtaaaacgac tgcgagatta taagcaatac gatttttgat ttgtgctcct tctgtagcat
    19741 tttctgtttc agcttcttct ttaggtgaaa catgaatacc cgctaataca cgtcccttaa
    19801 agctttttgc tgggattttc acttctatag gtatagttgt ttctgcatgt gctgatacat
    19861 tgatttcctt tttcgcaatg agcaaaacgt cacggacatc aaacggcaat gtctcatcta
    19921 acgttgggtt tttttgacta taatcgatcg ttccaccatc actcgttacc gccggattga
    19981 cagagacctc aaatgttcgt tcttcatctg aagaattgct gataattact tctagtgtct
    20041 gattttgttc cggtgccact aataaatcaa aataaccgat tgctttatct atttggttct
    20101 ctggaaatac cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg ctgattgacg
    20161 gaaaaatgaa aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt ttactttgat
    20221 tcattgtcca ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca cctatagtat
    20281 tttcaccgca gtttgttgaa tttatttcat actctatgct actgggccag ccacgaggtt
    20341 ccaagtcaaa tcagccgtga atgttccagc tgctgctttc ggcactttca gtgtgatatt
    20401 tgctggatca taaacagaaa gccatgttcc cagaccttga tcttttgctg ctgtaaatat
    20461 tggtgtcgct gcacttgctt tactcaaccc agttacagtt tgactagttg gtccagtgga
    20521 ttcgttatct gacgtacttt taacagtgga tgttccaaaa tctaattcag cgccttgcaa
    20581 aacttgtgaa ccgtttacaa aatctgaaac cgaaactgtc acgttccacc cttgtgcttg
    20641 gccatctgca cctacacctc gacgatctga tacttggaga taaggtgtat cgttttttga
    20701 agcgtctact tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa atggcaattc
    20761 aggaactaca tccaatgtta aagaacctgt ctggccagtt gctggatttt ccggatctgc
    20821 tggctctact ggttttgaag gatcagtagg gttaagaggc tcaacggtat ttgttggttc
    20881 agtaaaggta atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg gtgttgccaa
    20941 gccgcctaga agaagtgttg aaagtaataa tgtcccaatc atgttctttt tcataataaa
    21001 tcactccttt ttcttagtga cctaagtata gtagagacag tatgcaaaaa atcctcatga
    21061 attcatccgc ttacttgaaa ttatgaccct ttttgtataa aagagaaaaa aacaataaaa
    21121 tcgtttacat gaatattctt ccttttttat taagtatgaa aaaatatttc aattttattt
    21181 tttcgtttca aattattcta ttagtgacat ccttgaacta taaaaatagg agtacggcat
    21241 catctgccgc actcctattt ttttcgtcat ttatttctta aataaaacga ttctcacgca
    21301 attttatttt tttgatatct tctactgttt gtgttccagc taattgcatg accatttcca
    21361 attctgtttt aaagaaatca aacacttgat ggactcctgt acttcctcct aaagacaatc
    21421 cataaataac gggtctgcca atagcaacaa gatcggcacc tgatgcgatt gctttgaaaa
    21481 catgttgtcc acgtcgtaca ccgctgtcaa acacgattgg tacacgttta tctactgctt
    21541 ctgccacata ttgcaacgaa tcaaacgcag caggcccccc atctaattga cggccaccat
    21601 ggttagatac ccaaattcca ccagcaccag attctaatga tcgatagaca tcttcttctg
    21661 attgaacacc tttcacgtag actggcaaat cagaatgagc agcgataaat tctacatctt
    21721 ttggactaag tttttgtttg gaagatttat atacagcatc catcgtttgg cctactcctg
    21781 attgataagc ctgaacgatc ggcattggca gcgggaaggt aaacccgttt ctgcggtctg
    21841 tttcacggtt tccgccaact gttgcatccg ctgtcaatac gattgctttt gctccattac
    21901 gcttggccac ttcaagaata tccaaattga ttccattatc tttgctcata taaaattgaa
    21961 accattgcgg tgctttctcc ccgcctgctt ctcttatctc ttctaaagta caagaagcgt
    22021 aagaacttgc tgtatagatc gtcccaaaat ctgctacacc ttttgcagaa gctttctccg
    22081 ccttcacatg agctaatcca tgagcagcaa ctggtgccat gatgattggt gcagtcagca
    22141 tttcttcgtc aaaatgtgta gttgtatcgg gtaattctac atctcttaat acatgaggaa
    22201 taatcaaacg atgattgaat gcccgttcat tttcttgata agtaaataag tcgccagcac
    22261 cactgctgat gtaaccataa ccaccagctg gaatcacttg tgaagccgcg atttctaaat
    22321 cttccatatt gataaaatct actattcctt catttgtacc tgcttgatat gttttttcca
    22381 tctcaatgcc ttctctctat aaaatttgaa tgatgaacaa tatgatagcg tttacacttt
    22441 ttatattact cttcgacatt gatacaggca aataatctgt ttacatttca gccattccag
    22501 atgtcactac tctggaagaa atgattgtaa taaaaataga taaaaaaccg atgggacttt
    22561 gacaaaagta gtgtcaaagt cccttttttt gaataaacgg tgttcaaaag ccattttctc
    22621 ggtattaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttact
    22681 actcgaaact aagcgacttt ttcacaacct ctcgataaaa cggtgttcaa tcagct
//
LOCUS       DACECJ010000041        22396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.041, whole genome shotgun sequence.
ACCESSION   DACECJ010000041 DACECJ010000000
VERSION     DACECJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.041"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IWO09_001625"
     CDS             complement(218..1015)
                     /locus_tag="IWO09_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0264216.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IWO09_001626"
     CDS             complement(1012..2196)
                     /locus_tag="IWO09_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0264217.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IWO09_001627"
     CDS             complement(2221..3468)
                     /locus_tag="IWO09_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0264218.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO09_001628"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO09_001628"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0264219.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IWO09_001629"
     CDS             5448..5762
                     /locus_tag="IWO09_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0264220.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IWO09_001630"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IWO09_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0264221.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IWO09_001631"
     CDS             6447..7091
                     /locus_tag="IWO09_001631"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0264222.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IWO09_001632"
     CDS             7106..7435
                     /locus_tag="IWO09_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264223.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IWO09_001633"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IWO09_001633"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0264224.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IWO09_001634"
     CDS             8423..9247
                     /locus_tag="IWO09_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0264225.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IWO09_001635"
     CDS             9240..9587
                     /locus_tag="IWO09_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0264226.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO09_001636"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO09_001636"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0264227.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IWO09_001637"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IWO09_001637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0264228.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO09_001638"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO09_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0264229.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO09_001639"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO09_001639"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0264230.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKNQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IWO09_001640"
     CDS             15244..16011
                     /locus_tag="IWO09_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264231.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IWO09_001641"
     CDS             16001..18043
                     /locus_tag="IWO09_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0264232.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IWO09_001642"
     CDS             18324..18473
                     /locus_tag="IWO09_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0264233.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IWO09_001643"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IWO09_001643"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0264234.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IWO09_001644"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IWO09_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0264235.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IWO09_001645"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IWO09_001645"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0264236.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..>22396
                     /gene="dltD"
                     /locus_tag="IWO09_001646"
     CDS             21481..>22396
                     /gene="dltD"
                     /locus_tag="IWO09_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0264237.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLD"
BASE COUNT         6994 a         4031 c         4639 g         6732 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgat ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatc
//
LOCUS       DACECJ010000042        22026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.042, whole genome shotgun sequence.
ACCESSION   DACECJ010000042 DACECJ010000000
VERSION     DACECJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.042"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..1371)
                     /locus_tag="IWO09_001647"
     CDS             complement(61..1371)
                     /locus_tag="IWO09_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0264238.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IWO09_001648"
     CDS             complement(1383..2801)
                     /locus_tag="IWO09_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0264239.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IWO09_001649"
     CDS             complement(3041..4609)
                     /locus_tag="IWO09_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0264240.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IWO09_001650"
     CDS             complement(4613..4894)
                     /locus_tag="IWO09_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0264241.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IWO09_001651"
     CDS             complement(4891..5814)
                     /locus_tag="IWO09_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0264242.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IWO09_001652"
     CDS             complement(5811..6689)
                     /locus_tag="IWO09_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0264243.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IWO09_001653"
     CDS             complement(6676..8013)
                     /locus_tag="IWO09_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0264244.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IWO09_001654"
     CDS             complement(8016..9119)
                     /locus_tag="IWO09_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0264245.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IWO09_001655"
     CDS             complement(9193..10701)
                     /locus_tag="IWO09_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0264246.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IWO09_001656"
     CDS             complement(10667..11359)
                     /locus_tag="IWO09_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0264247.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IWO09_001657"
     CDS             complement(11381..12355)
                     /locus_tag="IWO09_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0264248.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IWO09_001658"
     CDS             12655..13527
                     /locus_tag="IWO09_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0264249.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IWO09_001659"
     CDS             complement(14109..16307)
                     /locus_tag="IWO09_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0264250.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IWO09_001660"
     CDS             16539..17531
                     /locus_tag="IWO09_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0264251.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IWO09_001661"
     CDS             17591..19012
                     /locus_tag="IWO09_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0264252.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IWO09_001662"
     CDS             19005..20504
                     /locus_tag="IWO09_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0264253.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IWO09_001663"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IWO09_001663"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0264254.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DACECJ010000043        21988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.043, whole genome shotgun sequence.
ACCESSION   DACECJ010000043 DACECJ010000000
VERSION     DACECJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.043"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWO09_001664"
     CDS             <1..395
                     /locus_tag="IWO09_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264255.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IWO09_001665"
     CDS             398..1147
                     /locus_tag="IWO09_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0264256.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="IWO09_001666"
     CDS             1159..1428
                     /locus_tag="IWO09_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264257.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1590..1892
                     /locus_tag="IWO09_001667"
     CDS             1590..1892
                     /locus_tag="IWO09_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264258.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="IWO09_001668"
     CDS             1889..2050
                     /locus_tag="IWO09_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0264259.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="IWO09_001669"
     CDS             2047..2352
                     /locus_tag="IWO09_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264260.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="IWO09_001670"
     CDS             2352..2708
                     /locus_tag="IWO09_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0264261.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="IWO09_001671"
     CDS             2668..2913
                     /locus_tag="IWO09_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264262.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="IWO09_001672"
     CDS             2910..3329
                     /locus_tag="IWO09_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0264263.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="IWO09_001673"
     CDS             3326..3883
                     /locus_tag="IWO09_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0264264.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="IWO09_001674"
     CDS             3880..4176
                     /locus_tag="IWO09_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264265.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="IWO09_001675"
     CDS             4253..4666
                     /locus_tag="IWO09_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0264266.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="IWO09_001676"
     CDS             5012..5386
                     /locus_tag="IWO09_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264267.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5616..5960
                     /locus_tag="IWO09_001677"
     CDS             5616..5960
                     /locus_tag="IWO09_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0264268.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="IWO09_001678"
     CDS             5965..6246
                     /locus_tag="IWO09_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264269.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="IWO09_001679"
     CDS             6349..6663
                     /locus_tag="IWO09_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0264270.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="IWO09_001680"
     CDS             6641..8335
                     /locus_tag="IWO09_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0264271.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="IWO09_001681"
     CDS             8355..9533
                     /locus_tag="IWO09_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0264272.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="IWO09_001682"
     CDS             9496..10182
                     /locus_tag="IWO09_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0264273.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="IWO09_001683"
     CDS             10182..11342
                     /locus_tag="IWO09_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0264274.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="IWO09_001684"
     CDS             11352..12227
                     /locus_tag="IWO09_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264275.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="IWO09_001685"
     CDS             12224..12535
                     /locus_tag="IWO09_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264276.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="IWO09_001686"
     CDS             12525..12878
                     /locus_tag="IWO09_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0264277.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="IWO09_001687"
     CDS             12868..13269
                     /locus_tag="IWO09_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264278.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="IWO09_001688"
     CDS             13262..13666
                     /locus_tag="IWO09_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264279.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="IWO09_001689"
     CDS             13678..14286
                     /locus_tag="IWO09_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0264280.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="IWO09_001690"
     CDS             14306..14668
                     /locus_tag="IWO09_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264281.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="IWO09_001691"
     CDS             14671..14853
                     /locus_tag="IWO09_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264282.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="IWO09_001692"
     CDS             14870..18289
                     /locus_tag="IWO09_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0264283.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="IWO09_001693"
     CDS             18340..19077
                     /locus_tag="IWO09_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0264284.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="IWO09_001694"
     CDS             19087..21378
                     /locus_tag="IWO09_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264285.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="IWO09_001695"
     CDS             21402..>21988
                     /locus_tag="IWO09_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264286.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DACECJ010000044        21912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.044, whole genome shotgun sequence.
ACCESSION   DACECJ010000044 DACECJ010000000
VERSION     DACECJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.044"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO09_001696"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO09_001696"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0264287.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWO09_001697"
     CDS             complement(1172..1867)
                     /locus_tag="IWO09_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0264288.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWO09_001698"
     CDS             complement(1880..2596)
                     /locus_tag="IWO09_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0264289.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWO09_001699"
     CDS             2926..3534
                     /locus_tag="IWO09_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0264290.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWO09_001700"
     CDS             3859..4182
                     /locus_tag="IWO09_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0264291.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWO09_001701"
     CDS             4364..5734
                     /locus_tag="IWO09_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0264292.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWO09_001702"
     CDS             complement(5864..7165)
                     /locus_tag="IWO09_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0264293.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWO09_001703"
     CDS             complement(7519..9510)
                     /locus_tag="IWO09_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0264294.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWO09_001704"
     CDS             complement(9654..9920)
                     /locus_tag="IWO09_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0264295.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO09_001705"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO09_001705"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0264296.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO09_001706"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO09_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0264297.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWO09_001707"
     CDS             13264..13827
                     /locus_tag="IWO09_001707"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0264298.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWO09_001708"
     CDS             complement(14080..14601)
                     /locus_tag="IWO09_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0264299.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWO09_001709"
     CDS             complement(14604..15446)
                     /locus_tag="IWO09_001709"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0264300.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWO09_001710"
     CDS             complement(15439..16164)
                     /locus_tag="IWO09_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0264301.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWO09_001711"
     CDS             16340..16636
                     /locus_tag="IWO09_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264302.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWO09_001712"
     CDS             17204..18796
                     /locus_tag="IWO09_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0264303.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWO09_001713"
     CDS             18809..19549
                     /locus_tag="IWO09_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264304.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWO09_001714"
     CDS             19717..21066
                     /locus_tag="IWO09_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0264305.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWO09_001715"
     CDS             complement(21194..21784)
                     /locus_tag="IWO09_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0264306.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DACECJ010000045        21832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.045, whole genome shotgun sequence.
ACCESSION   DACECJ010000045 DACECJ010000000
VERSION     DACECJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.045"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO09_001716"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO09_001716"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0264307.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO09_001717"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO09_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0264308.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO09_001718"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO09_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0264309.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO09_001719"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO09_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0264310.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO09_001720"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO09_001720"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0264311.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO09_001721"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO09_001721"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0264312.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWO09_001722"
     CDS             complement(8053..8523)
                     /locus_tag="IWO09_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0264313.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWO09_001723"
     CDS             complement(8569..8994)
                     /locus_tag="IWO09_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0264314.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWO09_001724"
     CDS             complement(9061..9330)
                     /locus_tag="IWO09_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0264315.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWO09_001725"
     CDS             complement(9341..10936)
                     /locus_tag="IWO09_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0264316.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO09_001726"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO09_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0264317.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWO09_001727"
     CDS             complement(14489..15049)
                     /locus_tag="IWO09_001727"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0264318.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWO09_001728"
     CDS             15402..16376
                     /locus_tag="IWO09_001728"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0264319.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWO09_001729"
     CDS             16546..17946
                     /locus_tag="IWO09_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0264320.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWO09_001730"
     CDS             complement(18024..18746)
                     /locus_tag="IWO09_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0264321.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWO09_001731"
     CDS             complement(18825..19331)
                     /locus_tag="IWO09_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0264322.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWO09_001732"
     CDS             19454..21253
                     /locus_tag="IWO09_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0264323.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..>21832)
                     /locus_tag="IWO09_001733"
     CDS             complement(21321..>21832)
                     /locus_tag="IWO09_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0264324.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6049 a         4791 c         3963 g         7029 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tg
//
LOCUS       DACECJ010000046        21498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.046, whole genome shotgun sequence.
ACCESSION   DACECJ010000046 DACECJ010000000
VERSION     DACECJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.046"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            179..880
                     /locus_tag="IWO09_001734"
     CDS             179..880
                     /locus_tag="IWO09_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0264325.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1489..2505)
                     /gene="tsaD"
                     /locus_tag="IWO09_001735"
     CDS             complement(1489..2505)
                     /gene="tsaD"
                     /locus_tag="IWO09_001735"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0264326.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2502..2966)
                     /gene="rimI"
                     /locus_tag="IWO09_001736"
     CDS             complement(2502..2966)
                     /gene="rimI"
                     /locus_tag="IWO09_001736"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0264327.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2973..3515)
                     /gene="rimI"
                     /locus_tag="IWO09_001737"
     CDS             complement(2973..3515)
                     /gene="rimI"
                     /locus_tag="IWO09_001737"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0264328.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3499..4323)
                     /gene="tsaB"
                     /locus_tag="IWO09_001738"
     CDS             complement(3499..4323)
                     /gene="tsaB"
                     /locus_tag="IWO09_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0264329.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4412..5392)
                     /locus_tag="IWO09_001739"
     CDS             complement(4412..5392)
                     /locus_tag="IWO09_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0264330.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5416..6900)
                     /gene="galT"
                     /locus_tag="IWO09_001740"
     CDS             complement(5416..6900)
                     /gene="galT"
                     /locus_tag="IWO09_001740"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0264331.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6912..7901)
                     /gene="galE"
                     /locus_tag="IWO09_001741"
     CDS             complement(6912..7901)
                     /gene="galE"
                     /locus_tag="IWO09_001741"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0264332.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8149..8316
                     /locus_tag="IWO09_001742"
     CDS             8149..8316
                     /locus_tag="IWO09_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0264333.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8378..10189)
                     /locus_tag="IWO09_001743"
     CDS             complement(8378..10189)
                     /locus_tag="IWO09_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0264334.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10186..10551)
                     /locus_tag="IWO09_001744"
     CDS             complement(10186..10551)
                     /locus_tag="IWO09_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0264335.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10714..11109)
                     /locus_tag="IWO09_001745"
     CDS             complement(10714..11109)
                     /locus_tag="IWO09_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0264336.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11127..12089)
                     /locus_tag="IWO09_001746"
     CDS             complement(11127..12089)
                     /locus_tag="IWO09_001746"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0264337.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12089..12301)
                     /locus_tag="IWO09_001747"
     CDS             complement(12089..12301)
                     /locus_tag="IWO09_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0264338.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12322..13020)
                     /locus_tag="IWO09_001748"
     CDS             complement(12322..13020)
                     /locus_tag="IWO09_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0264339.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13040..13582)
                     /locus_tag="IWO09_001749"
     CDS             complement(13040..13582)
                     /locus_tag="IWO09_001749"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0264340.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13714..14718)
                     /locus_tag="IWO09_001750"
     CDS             complement(13714..14718)
                     /locus_tag="IWO09_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0264341.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14715..15704)
                     /locus_tag="IWO09_001751"
     CDS             complement(14715..15704)
                     /locus_tag="IWO09_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0264342.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15701..16507)
                     /locus_tag="IWO09_001752"
     CDS             complement(15701..16507)
                     /locus_tag="IWO09_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264343.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16673..17629
                     /locus_tag="IWO09_001753"
     CDS             16673..17629
                     /locus_tag="IWO09_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0264344.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17706..18224)
                     /locus_tag="IWO09_001754"
     CDS             complement(17706..18224)
                     /locus_tag="IWO09_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0264345.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18312..18461)
                     /gene="rpmG"
                     /locus_tag="IWO09_001755"
     CDS             complement(18312..18461)
                     /gene="rpmG"
                     /locus_tag="IWO09_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0264346.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18689..19135
                     /locus_tag="IWO09_001756"
     CDS             18689..19135
                     /locus_tag="IWO09_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0264347.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19330..21225)
                     /gene="asnB"
                     /locus_tag="IWO09_001757"
     CDS             complement(19330..21225)
                     /gene="asnB"
                     /locus_tag="IWO09_001757"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0264348.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6474 a         4561 c         3701 g         6762 t
ORIGIN      
        1 tacacaaatc attcgcttca aaacaaatta ttcaatacca ttgtatattt tttacataat
       61 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
      121 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
      181 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
      241 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
      301 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
      361 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
      421 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
      481 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
      541 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
      601 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
      661 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
      721 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
      781 acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac
      841 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
      901 gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg
      961 gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat
     1021 attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg
     1081 atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga
     1141 acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg
     1201 taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac
     1261 ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa
     1321 ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc
     1381 aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc
     1441 ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt attcagataa
     1501 aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac
     1561 atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac
     1621 tagttctacc tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg
     1681 attggccgct acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg
     1741 aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt
     1801 tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt
     1861 taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta
     1921 attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc
     1981 taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat
     2041 gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag
     2101 aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc
     2161 catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga
     2221 aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa
     2281 atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc
     2341 tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat
     2401 ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt
     2461 ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt
     2521 tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt
     2581 ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga
     2641 cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac
     2701 aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg
     2761 acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa
     2821 agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt
     2881 gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca
     2941 tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata
     3001 tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac
     3061 cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc
     3121 gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt
     3181 cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac
     3241 gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga
     3301 ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta
     3361 tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt
     3421 acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa
     3481 aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt
     3541 ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt
     3601 ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc
     3661 ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa
     3721 tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa
     3781 caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca
     3841 gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt
     3901 atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt
     3961 atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga
     4021 accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc
     4081 aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg
     4141 tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt
     4201 atctattcct aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat
     4261 tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata
     4321 catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta
     4381 tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact
     4441 ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg
     4501 tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg
     4561 ttacatattt cgctacactg acatcattga agcccatcac agaaatatct tctggtacca
     4621 ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa
     4681 aaagcgcatc aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg
     4741 actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa
     4801 cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt
     4861 ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt
     4921 tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag
     4981 aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga
     5041 attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt
     5101 tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat
     5161 aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct
     5221 tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa
     5281 ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa
     5341 cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct
     5401 cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc
     5461 atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt
     5521 ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc
     5581 tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac
     5641 ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa
     5701 cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg
     5761 atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct
     5821 tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat
     5881 agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat
     5941 aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc
     6001 tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat
     6061 aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat
     6121 tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag
     6181 tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga
     6241 atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt
     6301 cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc
     6361 gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc
     6421 gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata
     6481 ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt
     6541 tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg
     6601 tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc
     6661 aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac
     6721 aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag
     6781 agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat
     6841 ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat
     6901 atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt
     6961 ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt
     7021 tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga
     7081 atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg
     7141 ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa
     7201 atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt
     7261 gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa
     7321 atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt
     7381 gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac
     7441 cacttcatca ttttttccat catcaattta ctttcaccat acggattttt aggattagct
     7501 ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag
     7561 aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat
     7621 acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca
     7681 gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta
     7741 tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg
     7801 agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc
     7861 gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt
     7921 agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga
     7981 aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta
     8041 aacattttca agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc
     8101 tcttttgttc cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt
     8161 gtatctggaa tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct
     8221 tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac
     8281 cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa
     8341 gactttctaa aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt
     8401 gtgctatttt atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg
     8461 cagttagcca atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt
     8521 cccctgaagc atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat
     8581 aacttcctgt attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt
     8641 ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat
     8701 agcgactgct gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa
     8761 tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt
     8821 tcgtccgaat gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc
     8881 atagtatttc tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg
     8941 gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt
     9001 tttccgcttc ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat
     9061 tctgataggc aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg
     9121 gatgctttcc gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct
     9181 ttggaatcgg caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt
     9241 ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca
     9301 ggttttcttt tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc
     9361 ctcgagggta ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg
     9421 atggatgata gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa
     9481 acaatccgac ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag
     9541 gcggtatctt cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat
     9601 ttttctttgc gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac
     9661 cgcttgctaa aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac
     9721 catcttttct tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa
     9781 attcttcttg caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag
     9841 attgctgatc tttttccaga cgattgattt cttctataat tagttgctta tttttggtta
     9901 ccgtctgttc gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct
     9961 gtgttaccaa tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa
    10021 cagaattcat taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat
    10081 tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt
    10141 ctgctacact taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc
    10201 catttcgata aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata
    10261 aacaaaagta atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt
    10321 cttttcttct tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc
    10381 tggtacatgg tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag
    10441 atctaactgc gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct
    10501 atagccatcc tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc
    10561 cttctttcta ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat
    10621 gaacggcaca actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat
    10681 tcggttttta aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc
    10741 agaccatccg tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa
    10801 tttatttgct gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc
    10861 ttttcgaata gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa
    10921 aatatgccca gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat
    10981 tccttctcga aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag
    11041 atagacttcc caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca
    11101 taaaaccatg taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca
    11161 atgacaccag cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc
    11221 gggaaagtaa attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc
    11281 gatactcctc cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa
    11341 gctaaaccta agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct
    11401 tgagccattt caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc
    11461 agttttgaat cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta
    11521 gcgctagata cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga
    11581 tccacagtga tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg
    11641 ccagcaacga tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg
    11701 ttttctccag cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata
    11761 gcgaatttga ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag
    11821 tttaagttat acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc
    11881 atgccgattc cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt
    11941 gattcaatga tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc
    12001 catttttgct gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct
    12061 aaatcaatcc caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa
    12121 ttcggtgctc tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac
    12181 ggctatcata cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac
    12241 ccacatagat aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca
    12301 tctttaagca cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat
    12361 acatattaca aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata
    12421 gcttgtctgt tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc
    12481 accgacatgt ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac
    12541 aataaataaa agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa
    12601 ccctaagata aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag
    12661 aacattcgct tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata
    12721 aatcaagaaa aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag
    12781 tatcagtgag ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa
    12841 acgccaccat tcattgaagt gaacaagata aggaccatac attccggcat aataagggat
    12901 ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc
    12961 ataggtccat aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat
    13021 gaaaacctcc catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct
    13081 ccggttgcca ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa
    13141 aattatttaa ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat
    13201 tgtagataat acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg
    13261 attgtggctc aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat
    13321 aaaacgccat ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt
    13381 ctgctcgttg gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta
    13441 tcacttttgc tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt
    13501 tttccttttt cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta
    13561 atcttacttt caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa
    13621 aaaaagaaaa atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc
    13681 tagaaatgat tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct
    13741 gataaaaagt gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg
    13801 tacttcccca cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga
    13861 accgaacaaa ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc
    13921 agcatgagga gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct
    13981 tgccaaagca acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa
    14041 tccttgcgcg cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc
    14101 tagcggaatt aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc
    14161 ccagattgat cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat
    14221 cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat
    14281 tcgattcata cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc
    14341 taaaacacca cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa
    14401 gaaacctaaa taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga
    14461 atctgccaat tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc
    14521 tcctgctaat aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa
    14581 agaagatgat tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt
    14641 accaatcatt aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt
    14701 ccaatatatt tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca
    14761 atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta
    14821 aaatccgcca acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga
    14881 tagtccgttc caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca
    14941 ggtccgacta acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac
    15001 cgcgttcgtt taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa
    15061 gatattgctt ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat
    15121 actgctaatt gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc
    15181 tgctgcagat taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata
    15241 gctacgccag ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa
    15301 ccaaatacga gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa
    15361 tcagaagctt taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg
    15421 attcctagta agcctgaatc tgctaaagga tttcttgtca ccccttgcat caatgcaccg
    15481 ctcaatgcaa aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt
    15541 cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct
    15601 gctttttcca cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca
    15661 aaacagacca aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct
    15721 cttttggcaa caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa
    15781 tcgttgcatc aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg
    15841 taccttctgt gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag
    15901 catgattaat gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag
    15961 tcaataactg taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca
    16021 agatcaagat atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc
    16081 caccagacag cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg
    16141 cccactcaat tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt
    16201 atcgtccgta agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg
    16261 ctaataatgc aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat
    16321 tgacaatgat ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag
    16381 attttccact tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag
    16441 acatgttttc aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc
    16501 tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat
    16561 ttcgtcaagc gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata
    16621 tcaggaataa taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt
    16681 aaaaacaaaa gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg
    16741 ctccccctct tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag
    16801 tactcataca gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg
    16861 gatcatcggc agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca
    16921 gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc
    16981 aacgatttct tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt
    17041 gatttcttct tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc
    17101 aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg
    17161 caaagctttg gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat
    17221 caagaaaact cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg
    17281 ggtgaccaac aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata
    17341 cgacgatctg gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc
    17401 tgactggtct gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct
    17461 tgtgaacagt gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc
    17521 agtgacagac aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc
    17581 tattgccagt caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa
    17641 aaaagcatct tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat
    17701 agattttatt cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga
    17761 tgggcatata gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct
    17821 gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc
    17881 cagatccgac cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag
    17941 atatcttctg agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc
    18001 gcattttctt tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga
    18061 agtgaaagat cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt
    18121 gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg
    18181 ccgatcagac catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat
    18241 gattttagat gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa
    18301 ctttaaaaac attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat
    18361 ttttgttttt ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt
    18421 tctttacaag aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt
    18481 acaagaatcc tctaagatga ctctcagact tgattatctt accatgattt tttctagatt
    18541 gctagtgttt ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta
    18601 cttctcaaac aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt
    18661 atgtttttta ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc
    18721 aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt
    18781 agatgaaggg taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg
    18841 gtatgaagat gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa
    18901 gacggatgaa cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt
    18961 agaatatgag gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca
    19021 ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc
    19081 aactttgttc atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata
    19141 tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt
    19201 cctctgccaa ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg
    19261 aaattgtttc ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt
    19321 tgattttcat caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac
    19381 aaagtagact ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc
    19441 tttacgtcct tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc
    19501 ttttgcaaaa tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa
    19561 ccaagctttg attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag
    19621 atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt
    19681 acttggtatt tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc
    19741 caaagaactt gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg
    19801 atgaagatcg acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta
    19861 atgaacagtc atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc
    19921 tgatgcttct gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc
    19981 aagtgattca tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc
    20041 taaattatag cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa
    20101 ttttgaattc gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc
    20161 tccagattgg actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac
    20221 gcaagaagga tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa
    20281 cgacatgtct ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt
    20341 tctggcttca atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc
    20401 tggtttcaat acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac
    20461 ttctacatca ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc
    20521 gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg
    20581 aatgtctatt tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc
    20641 tttgaagaat gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc
    20701 ttctttgttc aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc
    20761 aaacataaat gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct
    20821 tgcaccgaat aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat
    20881 cttttgcaat aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc
    20941 atgtgtttga aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata
    21001 gatttcgcca ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc
    21061 gccctctaag tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata
    21121 ttctcctgaa ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt
    21181 ttttttgttg tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc
    21241 taatataaaa ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac
    21301 gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt
    21361 acagaaaata aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt
    21421 ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac
    21481 acgacctaaa gatcgtgt
//
LOCUS       DACECJ010000047        21383 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.047, whole genome shotgun sequence.
ACCESSION   DACECJ010000047 DACECJ010000000
VERSION     DACECJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.047"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWO09_001758"
     CDS             <1..152
                     /locus_tag="IWO09_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0264349.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IWO09_001759"
     CDS             complement(230..763)
                     /locus_tag="IWO09_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0264350.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWO09_001760"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWO09_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0264351.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWO09_001761"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWO09_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0264352.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IWO09_001762"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IWO09_001762"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0264353.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IWO09_001763"
     CDS             complement(4488..5066)
                     /locus_tag="IWO09_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0264354.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IWO09_001764"
     CDS             5388..5858
                     /locus_tag="IWO09_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0264355.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IWO09_001765"
     CDS             complement(5966..7192)
                     /locus_tag="IWO09_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0264356.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="IWO09_001766"
     CDS             complement(7946..8146)
                     /locus_tag="IWO09_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0264357.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="IWO09_001767"
     CDS             complement(8280..8888)
                     /locus_tag="IWO09_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0264358.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="IWO09_001768"
     CDS             complement(8973..9443)
                     /locus_tag="IWO09_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0264359.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="IWO09_001769"
     CDS             complement(9491..10405)
                     /locus_tag="IWO09_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0264360.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="IWO09_001770"
     CDS             10551..11354
                     /locus_tag="IWO09_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0264361.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="IWO09_001771"
     CDS             11532..12479
                     /locus_tag="IWO09_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0264362.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="IWO09_001772"
     CDS             complement(12547..12840)
                     /locus_tag="IWO09_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0264363.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="IWO09_001773"
     CDS             complement(12868..13533)
                     /locus_tag="IWO09_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0264364.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="IWO09_001774"
     CDS             complement(13673..14305)
                     /locus_tag="IWO09_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0264365.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="IWO09_001775"
     CDS             complement(14312..15379)
                     /locus_tag="IWO09_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0264366.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="IWO09_001776"
     CDS             complement(15376..16107)
                     /locus_tag="IWO09_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0264367.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWO09_001777"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWO09_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0264368.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWO09_001778"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWO09_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0264369.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="IWO09_001779"
     CDS             18270..19439
                     /locus_tag="IWO09_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0264370.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..21328)
                     /locus_tag="IWO09_001780"
     CDS             complement(19706..21328)
                     /locus_tag="IWO09_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264371.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     IFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6600 a         4182 c         3366 g         7235 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatataa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctcat gtttttttat attacatata
    21361 gtatcattta tttttttaaa aca
//
LOCUS       DACECJ010000048        20651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.048, whole genome shotgun sequence.
ACCESSION   DACECJ010000048 DACECJ010000000
VERSION     DACECJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.048"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(103..1086)
                     /locus_tag="IWO09_001781"
     CDS             complement(103..1086)
                     /locus_tag="IWO09_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0264372.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1091..1738)
                     /locus_tag="IWO09_001782"
     CDS             complement(1091..1738)
                     /locus_tag="IWO09_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0264373.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1823..2455)
                     /locus_tag="IWO09_001783"
     CDS             complement(1823..2455)
                     /locus_tag="IWO09_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0264374.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2471..3022)
                     /gene="coaC"
                     /locus_tag="IWO09_001784"
     CDS             complement(2471..3022)
                     /gene="coaC"
                     /locus_tag="IWO09_001784"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0264375.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3019..3756)
                     /locus_tag="IWO09_001785"
     CDS             complement(3019..3756)
                     /locus_tag="IWO09_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0264376.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3753..4742)
                     /locus_tag="IWO09_001786"
     CDS             complement(3753..4742)
                     /locus_tag="IWO09_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0264377.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5000..5542
                     /locus_tag="IWO09_001787"
     CDS             5000..5542
                     /locus_tag="IWO09_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0264378.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5559..6644
                     /locus_tag="IWO09_001788"
     CDS             5559..6644
                     /locus_tag="IWO09_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264379.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6656..7462
                     /locus_tag="IWO09_001789"
     CDS             6656..7462
                     /locus_tag="IWO09_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0264380.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7463..8290
                     /locus_tag="IWO09_001790"
     CDS             7463..8290
                     /locus_tag="IWO09_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0264381.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8287..9360
                     /locus_tag="IWO09_001791"
     CDS             8287..9360
                     /locus_tag="IWO09_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0264382.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9788..12484
                     /locus_tag="IWO09_001792"
     CDS             9788..12484
                     /locus_tag="IWO09_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0264383.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12561..13049
                     /locus_tag="IWO09_001793"
     CDS             12561..13049
                     /locus_tag="IWO09_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0264384.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            13061..13555
                     /locus_tag="IWO09_001794"
     CDS             13061..13555
                     /locus_tag="IWO09_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0264385.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13576..14385
                     /locus_tag="IWO09_001795"
     CDS             13576..14385
                     /locus_tag="IWO09_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0264386.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14382..15203
                     /locus_tag="IWO09_001796"
     CDS             14382..15203
                     /locus_tag="IWO09_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0264387.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15254..17353
                     /locus_tag="IWO09_001797"
     CDS             15254..17353
                     /locus_tag="IWO09_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0264388.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17350..18468
                     /locus_tag="IWO09_001798"
     CDS             17350..18468
                     /locus_tag="IWO09_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0264389.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18507..19385
                     /locus_tag="IWO09_001799"
     CDS             18507..19385
                     /locus_tag="IWO09_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0264390.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19576..19728
                     /locus_tag="IWO09_001800"
     CDS             19576..19728
                     /locus_tag="IWO09_001800"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264391.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6319 a         3559 c         4393 g         6380 t
ORIGIN      
        1 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
       61 ttaaacgctt ctgtcccagc ctcttttttg atcagcatga ttttattcag ttatcttcaa
      121 aatgattttt ccgatgtttt tgtttgcttc catatattca tgcgcttgtt tgactgcgct
      181 gaattggaat gtacggtcaa tgattggccg aatcttgcca ttttctagaa aaggcccagc
      241 tttttgcata aaagtttgtg cgagctctgc tttatactga tcacttctag gtgtcaataa
      301 tgtaccaagc agttggacac gtttcccaat caatacacca aggtctatct caggcacgat
      361 ggtcccacct agcataccaa tcaagaccca acgtccatca acggctatag aagctaaatt
      421 cttttcccag taagaagcgc cgatgaaatc caaaataaca tcgactcccc gaccttcagt
      481 gatttttcgt acctcttctt gaaagtcttg ttttttatag ttgatcaatt catctgcacc
      541 aagttcttta caaaaggcga gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg
      601 agaaagctgt ctagctaatt ggatcgctgc tgttcccaca ccacttgctc ctgcatggat
      661 caagacagtt tcgccttctt gtaattttcc aagccagtaa agtgtttgat aagcagtcaa
      721 aaaaacttct gagattccag cagcagaaac atagtctagc gtatcaggga gaaggattgc
      781 acgatcggca ggcatcacag catattcggc ataaccacct agattgacta atccgtatac
      841 ttttgtccca ggagtaaaag tggagatatc acttttattc tcaactacct ctccagctac
      901 ttcgactccc aaaatagggt agggaggttt caaagagact gattctcttt tcataatatc
      961 tgttcgattg acagctgctg tatggacttt gatcagtaat tcgccttgtt ttggtttagg
     1021 aacaggcact tcaatttgtt ctaatacatc tacagaacca ggatgcttga ttgaaatggc
     1081 acgcatggat ctattcctcc tttgcccatt cttggaatgc tgtattcaac tctttgctac
     1141 cttcagaaag tactagagtc gtacgagaaa tatccgtttc ttccactaga gcggcaagta
     1201 cagcagcaac tgtagagcga gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt
     1261 gaatggaagt aacttcttca tcgttagtca atgtgactgg ttgaagaatg acaaaatcta
     1321 agttagttcg tttcagccat tcatctgcat aatgtttagt aatataataa tctgtcatgg
     1381 actcaggcca ttttgctggt tcatctgcat aaacagcact gaccatcaga tagcgagaga
     1441 catgcgcatt ttcagctgcg atcactgttt tgattgcgcc atccaagtct acttgcagaa
     1501 gattctttcc ttgagagccg gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg
     1561 tcattttttc cacggaccaa gttaaatcaa aagagacata cgaaacattc tcttttttta
     1621 ctaatgtttg ggaagcaaca tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta
     1681 aatcttttat caggtgtcgg ccaatttgtc cgtttgcacc tacaacaaaa attttcattt
     1741 ggatcatcct ttcgacgtca aatctttcca ttcaatagta acaccaaaag agaaaaaaat
     1801 cagagatcgg ctacgatgac tatcagttag taagagaagg gtgcttgaag tatcttaaca
     1861 agacattgca tactgctgca gtcgcaagac ctgcgacgat tccttcaggt ataccacttg
     1921 taaaaataac cgctagaata gctgaataaa cagcatttaa atttgttccg atgactttgg
     1981 cataatcttg ctggaataaa aagtagatca tattcatcac taaaatcgta ttggtcaatg
     2041 aaccggaaag tccagctaag aataaagaaa cggaatctgc tttttgtttc attagtttgc
     2101 gcagaccttt ataaacgaaa taaggaacca ccccaatcaa gatgcgagga ataaacgcga
     2161 tcagcaaggc tttccagctt ccatgttctg ttccgatgac tgggataaac ggagaaaaga
     2221 caaaagacaa aggtgtttgg ataactgtgc tacggatcat gctgatcagt ccaaatgtac
     2281 cgccaagaaa agcacctagt cttggtccta aaacgatcga agcaatgatt acaggaatat
     2341 gcatggttgt tgcattgatt ggaccgatag ggataaaacc taaaggcgta acagctaata
     2401 aaatcaaaat agctaaaaac atcgcagtta atgtaaagtt ttttgtattt ttcataagta
     2461 ctcctttttt ctaagcattt ctttcattaa gagtcagcat gacctgttcg atgatcgtct
     2521 gaatatcagc taaggcacct tttccaaaat ctccacaagc taaaagagat tctcttggtt
     2581 caatttcctg atagccgaca gatctcaatg tttccagatt acgctgagtg atcgggtttt
     2641 gatacatatt cgtgttcatg gcaggggcaa tcaattttgg tgttcccact ggaagcgcca
     2701 ttgcgactgt tgaaagaaga tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg
     2761 ctgtagcagg cgcgatgaga aacaagtcag cttgttttgc tagttcgata tgattgatta
     2821 cctgcggatt tgattcttgc atgacgtctg tatggactgc gcgcttcgac agagattgca
     2881 aggttaatgg cgtgataaaa tgtgtgctgc ttttagacat aatcacatct acctgggcac
     2941 ctaatttgac gaattgactt gtaagatcag cgcttttgta agcagaaata cttccagtaa
     3001 cgcctagtaa gatctttttc attttgattc ctccagtcta attttctcag taatcaatgc
     3061 agcaatctca gatttacttt gagcttcttc cactgttcca tctttagata agagatagcc
     3121 gtggtgtgtc tcgccatgga tttccgtcaa atcatttgcg aaaataaaat cagttttgtt
     3181 atttatcaga ctattttttg cgactgccag aagagattcc tttggtacat ctactaatag
     3241 tttgaaacca atgatttttg cttttggctg ccattcacgt agataagaaa tgatttttgg
     3301 tgttttcttc aaaacgagca caagatgctc ggtgttagaa gagatctttg aaacagtggt
     3361 atctgtctgt tcattttccg caaaccaatt gtccagttct tcttgcgttg aagaagtggg
     3421 taggtttttt gccagctgtt cttctgaaaa actaaaagca ggagtaaaat cactgactgc
     3481 catactgtgg ataatcgcat cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa
     3541 taagtccagt gcagattcga tcgtataaat cgttatattg gagtgtcttt tcggtttcag
     3601 tgcttgtttc gtagtcacgt aatcgattgt ggtatccgga ttggcagcta aataatcagc
     3661 taatgcttgc cctaatcgtc ctgtcgaatg atttgtaata gcacgtactt gatcgatttt
     3721 ttcagtcgtt cctccagctg tgattagaat cttcatttat ggtcacctac atttactagt
     3781 gttcttcctt ggtgtgttcc agcaagtagt ttagatgctg tttcaggcaa ttgatccaga
     3841 gtaatctctt ggacgaccag ttcatctgca atctgcaaat ccgccatctg ttgccataaa
     3901 gaaagacgtt gatctattgg tacattgaca gaatcaattc caatcaactg gatgtttcgc
     3961 agaataaaag gcaagacagt cgcgtttagt ttgattcccc cagcattgcc acataaagct
     4021 actgcaccgt tataagagat caatggaaga agactgctta gctgctcgcc acctactgtg
     4081 tcgatgacat agtcgatttg ctgttttcct aaagcttttg ttgtctctgg cagaaattca
     4141 tcaggtcgta cgacttgcaa agcacctaga ctttttagcc atgtgacaga ctcttctttt
     4201 ctggaaaagg caatgattga ctggaatcct agtttatgta acagggctat cgctgtactg
     4261 cctacaccgc cagaagcacc agtaacgaca acttttttat cggaggtcat accttgacgg
     4321 attaaagcgt tcacacacaa catagcagta aaccctgctg taccaaggat catcgcttga
     4381 cgcagcgtca tattttttgg tagaggaact aaccagtctc ctggtacttt ttgatattgg
     4441 ctataaccgc ctggatgggt gacgccaagc ccaaaaccag tagcaagtac aagatcttct
     4501 tttgaaaaac gtggattgtt actttggacg acagttccgg ataaatcaat ccctggagtc
     4561 atcggatatg ttcgtatcac ctgaccagac tcgctcattg ccaatgcatc tttataattg
     4621 acatcagaat aggcaatttt taccaagact tcattttcag ccaactcagt aaatggcaga
     4681 tcactgattg ctgtatggaa aacaggttcc tttattacct gaaaagatcg aaaagcgttc
     4741 attcgtgaga cactcctttt aaaattttct tttatcatac cataaagcct actcttttat
     4801 tcttgttctt ccgtaaattt agacaaaaat taggtgaaaa ataaagaatc aaacaactag
     4861 aatttattcc aaaagataaa gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt
     4921 ttcaataaat ttcgtatact aatacgagtg gtagtaaaca ggaagtttac tattagaaaa
     4981 cttcgattcg gaggaaatta tggaaattgg tgagaagtta agaaatttgc gcatccaaaa
     5041 gaatttgaca caagaagaac taggcgaacg cacagatttg tcaaaagggt atatttccca
     5101 gttggaacga gatttaagtt caccatcaat ggaaacattc ttttctattc ttgaagtctt
     5161 aggtgtgact ccagaagaat tttttcatca agaagcagcg aatctgcaag tggtttattc
     5221 aaaagaagat catacggttt atttcgatga agaaaacggg tatgaattgg aatggctcgt
     5281 ggctgattcg aatgaaaaag aaatggagcc tgtcttgctg acttttgaag gtgcaggaga
     5341 atataagacg tttgaacctt cattatctga aacgtttatt tttgtattag aaggggaatt
     5401 agagttaagc ttaggagaga cggtctatgt ggcaaaagca ggacagtcga tctattatca
     5461 agcgacaaaa caacatcaat taaggaatca tctcaaaaaa cgaacaaaag tattgattgt
     5521 tgcaacgcaa tcatatttgt aatatagggg gtaaaaacgt ggggaaacca atcatttcat
     5581 ttgatcaagt agtgaaacgc tatgatgatg aaacagtatt aaaaaaagta agttttgaga
     5641 tcgaacaagg aaaattttat acactgcttg gtccttccgg ctgtggtaaa acaacgatcc
     5701 tacgtattat agctggattc accgaagcta cagaagggga catctatttt gaagggaaac
     5761 ggttgaatga tttgccagct aataagcgac aagtaaatac agtcttccaa gattatgcac
     5821 tttttcctca tatgaatgtt tttgataacg ttgcttttgg attgaagatc aagaaaatgt
     5881 ccaaatcaga tatcgaaaag aaggtaaaag atgctttgcg tatggtccag cttcctggat
     5941 acgaaaaacg ggagattagt gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac
     6001 gagcaattgt aaatgaaccg aaagtattgc tattagatga accgttatcg gcgcttgatt
     6061 tgaaactacg tacagatatg cagtatgaac tgcgggatct gcaacaacga ttaggaatca
     6121 cgtttatttt tgttactcat gaccaagaag aagcgttggc aatgagtgat gagatttttg
     6181 tcatgaataa agggaaaatc gtccaaagtg gtacacccgt agatatttat gatgagccaa
     6241 tcaatcattt cgttgctgat tttgttgggg agagcaatat cgtggacggt gtaatgatcg
     6301 aagataatct agttgagttt gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc
     6361 taaatgaacc agttgaagtt gttttacgcc cagaggattt gacgatcacg acaccagata
     6421 aaggaaaact agtcgtaacg gtcgatactc agctgtttcg tggtgtccat tatgaaatca
     6481 tctgttatga tgaacagcaa aatgaatgga tggtccattc aacgaaaaaa gctaaagaag
     6541 gaagcaaagt aggattggcc tttgagccag aagatatcca tgtcatgcgt ttcaacgagt
     6601 ctgaagaaga attcgatgca cgtcttgaca gctacgaaga atagggggga caagaatgaa
     6661 aacaatgcgc aggatctatt ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc
     6721 gcctgtatta atgatcattt atcaatcttt ttttgatatg aatgggcaat tcacgctgaa
     6781 taattatcta tcttatttta cgtcggggac atcgctttct atgacgatca attctgtatg
     6841 gtatgcattt ttgatcacgt ttttcacgct gatcatcagt tacccgactg cttatttttt
     6901 aacaaaactg aagcacaagc aattatggct gatgctgatt attttgccta catgggtgaa
     6961 tctgctgctt aaagcttacg catttattgg gattttcagt gtgcacggca gcgtgaatca
     7021 tttctttgat tttatcggga tcgggccgaa acagatatta ttcacagact tcagttttct
     7081 gttcgtggca acgtatatcg agattccatt tatgattatg ccaattttca atgcattgga
     7141 agagatgaat ccttctttga tcagtgctag tagagattta ggcgcgaata gctttgagac
     7201 cttccgacga gtgatttttc cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt
     7261 atttattcct tctctttctt tattcatgct gactcgtctg attggcggaa atcgggtgat
     7321 cactttagga acggctatcg aacagcattt cttggttaca caaaactggg ggatgggttc
     7381 tacaatcggt gtgattttaa tcattgctat gttcatcgtg atgctcctga caggcgagaa
     7441 gaaaaaagga ggtcgctcat aaatgaaaaa atttaaatgg tcgtatcttt atttagggat
     7501 cgtcttttta ttcttatatg caccgatctt ctatctgatt ttttattcct tcaatgaagg
     7561 aggaacgatg aacagcttta caggatttac ttgggaaaat tatgaagctg tttttgaaga
     7621 tacacgtttg atcattatcg tattgaatac gttcatgctg gctttcttat ctgcattgat
     7681 cgccacgatc atcgggacat ttggggctat gggaatctat tacacgaaaa aacgacgtgc
     7741 acggacagcg ttgttgagca tgaataacat cttattagtc tctcctgatg ttattatcgg
     7801 tgctagtttc ttgattttct ttacactact aggattcgga ttaggttttc agagtgtatt
     7861 gctttcccat attgctttca gcattccaat cgtcgtcttg atggtcttgc caaaattgca
     7921 ggaaatgaac gattcaatgg tcgatgcggc tagagacctt ggtgctaata atttccaagt
     7981 agtcaaaaat atcattttgc cttatctttc tccaggaatc atagctggat attttatggc
     8041 atttacctat tctcttgatg attttgcggt tacatttttc gtcacaggaa atggctttac
     8101 gacgctctca gtagagatct attctcgtgc aagacaagga atcagtttag aaatcaatgc
     8161 gctaagtgcg ttagttttct tgttctccat gatattggtc attggatact actttattag
     8221 taaagaaaac cgtccaaaac ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg
     8281 gctgcgatga aaaaattaca atctctgttt ttaggtattc tcatcattat tgcggtgctg
     8341 ctgcttggtg ttcgtcaact tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc
     8401 atctataact ggggagatta tatcgatcct agtttgatca cgaaatttga aaaagaatat
     8461 ggatacaaag tgaattatga aacatttgat tctaatgaag cgatgttcac aaaaatccag
     8521 caaggtggga caaactacga catcgctata ccaagtgaat atatgatcca aaaaatgatc
     8581 aaagaaaaac tagtacttcc attagatcat tcgaagatca aaggattaaa taatattgat
     8641 ccacgatttt tagacttgga ttttgatcca gataatcagt attcgattcc atatttttgg
     8701 ggaactttag ggatcgtata taacgataaa ttctttgatg caaatcaaat taagcattgg
     8761 gatgatttgt ggaagccgga attaaaagac agtttgatgc tgattgacgg tgcacgagaa
     8821 gttatggggc tttctttgaa tagtttaggc tattctttga atagtaagaa tatggcacag
     8881 ttgacagaag cagcaaataa attgaatcat ttaacaccaa atgtcaaagc aatcgtggca
     8941 gatgagatca aaatgtatat gatcaatgaa gaagcctccg tggcagtgac attttctggc
     9001 gaagctgccg atatgatgtg ggaaaatgaa catttacatt atgtgatccc accagaagga
     9061 tccaatcttt ggtttgataa tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc
     9121 tatgatttca tcaactttat gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt
     9181 ggttattcta caccaaatga agaagcaaaa gcgttattgc caaaagacat ttcgtcggac
     9241 gaacaattct atccatctga tgacacgatc tcccatcttg aagtctacaa agatctagga
     9301 tcgaaatacc taggcatcta taatgaccta ttcttagaat tcaagatgta taggagataa
     9361 tggcaaaact cagtaagaat ggggacttat caacgttcta aagagctaaa aatcgtcctt
     9421 tttctatctg agttttccaa tcttctacca aaagcggatg aaacgccgct ttttttgtgt
     9481 tattttattt tttctaccac gtttttctac cagagaaaag tggacaaaac cattgtaaaa
     9541 tagggcccga gacgggctag taaatctaag attgaaaaag ttacagaagg ataaagtaat
     9601 tggaaactat gcaaatatac ttaacccttc tgtaatctat taataaaact aatatatgaa
     9661 tccagttcaa tatttgtagc tatgaaaaaa cataaaaatc atattagata aaaactaaat
     9721 tggtagataa attattttgt cagatatctg aggcaaatat tcgactattt gtgtttcaaa
     9781 gtaaaaattg gaatacaaat atagaataga catattgcag ccgatagttt attatgcttt
     9841 tagacacatt aaactggagg aaaaaatgaa aaagagcgct aaagatcgag tttttgatga
     9901 gctgatacga ctaacaggga atatgtcttc tgtcactgcc gagaaaattt cgaagaatct
     9961 aggtatctct cgtcagaatg ccagccatta tttgacgaga ttagtggaag ataaaaaggt
    10021 cgagaaatta ccgggaaagc ctgttttgtg gaagccttta gatgaatatg cggttgtaga
    10081 taatacggag caaataaacg aagcctttca ttctgtagtt ggtcatgacg gaagtctgag
    10141 agaggtcatt caaaaatgta tagccgctgt caaatatccg cctaacggac tcagtgtatt
    10201 aataaatgga gccacgggtg ttggaaaaag ttttttagcc acaaaaattt ttgaatacgc
    10261 tatccatgag caaattattg aaaaagatgc accatttgct atcttaaact gtgccgatta
    10321 tgcggacaat cccgaactgc tttcggcaac actgtttgga tacaagaagg gggcctttac
    10381 gggggctgaa aaggataccg atggtttatt ggcaacggcc aacaacggct atctttttct
    10441 cgatgaggtc catcgcctat cgaaagaaaa tcaagaaaag ctctttctat ttattgatac
    10501 tggaaactat cgtccagtcg gtgaaaatgt aaattggcat tctgctaagg ttcgttttat
    10561 tttcgccacg acggaaaaag gtgaaaatta tttattagac acctttgatc ggcgtattca
    10621 aattagtgtt atgttgccaa cttttgaaga tcgccccatt cgagaacgat tagagctgat
    10681 tcaattattt tttcaaaatg aagcggatgt tttacaaaaa gatattattg tttcaagaga
    10741 agccttgtct ttaatgttat ctcatccctt tagtgggaat attggtaagc tgaagaatat
    10801 cattaaaatc agttgtgcgg atgtgtatag tcgaacacaa gaagaaccat taacgattgg
    10861 gaaaaatgag atcttgattc agttaaatat gctggaatcc gaagtagaaa gcttgccgat
    10921 tgctagttta ttgataaaga aaaatcaaaa gataaaaacg gatcgaatca aaccgtttga
    10981 ttatgattcg attttggaaa aaattactca gctgataaat gaaggtaatt tcaatgaaaa
    11041 tttttcttcg ataaagaatg aagtacagca tttggtgcat cacattgata aggattcttt
    11101 tttatcaatg agtagaaaat tgatgattca gatgtttaga aaggtctggg tatccgtttt
    11161 aggaaaaacg tacggattga cgacgagtgg acctgttgca gaaattatta gcagatccta
    11221 tgtcatttcc ccaagctata agacagactt aaccgggata aatcgtacat tagctgcaaa
    11281 tctacctcgg acgacttact tatgtaatca gttcttggag catttaccac cgctctcaga
    11341 aaaggacgag aactttctaa gactgcttat tgtcgtgact ttatctgatt atgtagatga
    11401 atccatcgag ctgaagggcc tgttgcttgc tcacggagat agtacggcta gcagcattca
    11461 agcagtagtg aatcaattat gcggcaatta tgtttttgaa gcgttggata tgccagtaga
    11521 gagtaatgtg tcagagattg tcgaatcgac aaggaatttc gtgaaacggc aagtgaatac
    11581 agatagtcta gtgctgatcg tagatatggg ctctttaaat caaatttaca cacagatcaa
    11641 gaacgattta aaaggggagt tacttcttct gaataatttg acgacttcca ttgcacttga
    11701 tattggcttg aagatgacga tgaatgcgcc atttaaacag attgcggagg atgcagcaga
    11761 caagtattca atcaatgttc aatactttga aggcttttct caaagtagca acattatcat
    11821 ttcctgtatg tcgggtttgg ggatttcgga caaattgaaa gatgtttttc gtcatcattt
    11881 atcggatgaa tccatcgaag ttttcacgaa ggactacaga gaactacgtg aattaatcga
    11941 ccaaaatgat gaaacttatt ttgacaagac aaaattggtc atcacgactt cggatcttcc
    12001 acgttccttt tcaattccta atattaatgt ttatgacatc ttagacggtg atggagcagc
    12061 caatttggag catattttga gtcctgaaat gaagccggca gaatttaacc gcttgattca
    12121 ggagctagta aaattctttt cgttggaagg aattgcggac cgtttgaact ttctgaaccc
    12181 aacgatcgtg atgagtgaag tggaaacagt catttctaaa tatgaaaatt actatcattt
    12241 cactttaaat ggccgtgtaa aactaaatct ctatatgcat acggcattga tgatggaacg
    12301 tttattcttg gctcggacag aaaaatctga agaaccgaat tatgatttga aaccagatga
    12361 agatgaattt tattatgttt ctagaagtat ttttcaatca atggagatga aatataattt
    12421 taaagtcaat gcttatgagc tttcgctgct ctatgaatta ttaggaccgt atattcaaaa
    12481 ataaagtgtc taaaaaagtg tctaatctgt tttttttagg ttagacactt tttttaatgt
    12541 gaattaacgt gattaaacca atgtttttaa aagttggcac aggaattgct tatataaaag
    12601 tgaacagaaa gaaaggaatt aaccatatga agaaaattct aatagcgact cacggtcact
    12661 tagcttcagg ctttcttagc agtattcaat tactgacggg gaaggttcaa gagattacgg
    12721 tgattaatgc ttatgtggat gagtgtgatt ttgaacagga gttgaatgta tttgtaaacc
    12781 aggtgaacga gcaggatcag atttttgttt ttaccgattt atttggcgga agtgtcaatc
    12841 agaagattac caaaaccttt ttggagaaag agattgctgt aactattttg tctggcttta
    12901 atctgccaat tctgctcgaa atagtattgg caaccaagga attaacaaag gaggatattc
    12961 aggaactggt aaataaatgc caacaggaat taaagatgac cattcttgat gaggtacatg
    13021 tagtaaatga agaggaagat tttttttagg aggaagaaaa atgattaaag cagtgcgaat
    13081 tgatgaacga ttaattcatg gacaagtagc gatgacttgg acaaagagtc taaatttaac
    13141 cgggttagta gtagcaagcg atgaggcggc taccaacgat attcaaaaga tgacattgaa
    13201 gatggctgca ccatcgaatg tcaaagtcat tattaaaaca gttgatgacg cgattcattt
    13261 gttaaaagat ccgcgggcga aggacatgcg cttgatggtt ttagtcccta atgtcaaaga
    13321 tgcggtaaaa gttgcgaaag ctttcccaac ggaaatcgaa ttaatgaacg tggggaatgc
    13381 tgggaaaatg acaccgccat cggaagataa gaaaacctta tcaaaggaag tgatgttaac
    13441 gacgtcagaa attcaagact taaaagaatt agttgaactt tatccagata ccgtttttca
    13501 gcctacaccg gcgatggaaa aacgttcagc tatctctatt ttgagtgatt tttagaaaaa
    13561 ggaaggagaa aaattatgta tgatgcattt gtagtcgctt tggccgtttt tattggaatt
    13621 gctggacatg aaatgttcgg gatgtccatg ctgagtcgtc cgattgttgt tgctccgtta
    13681 gcaggagcgt tgatggggga tttgcagacg gggttgattg tcggggcgtc actagaagcc
    13741 atctttatgg gtgtcgtcaa tatcggaatc tctagtacgg cggagccttc tttggctgct
    13801 gggctggcta cagcgttcgc aatcaaaatg ggaggaaacg tggatgcgat tattcccatt
    13861 gtctttccac tagcagtatt aggattacag ttaatgaata tgattttttc ttttgtatgt
    13921 ggaccgatgg ctgctaaatt tttcaaattt gcaaaatctg gtgatcaaaa aggaatcatc
    13981 aatctgcact tctttatgtg gtttgtccac tatgcgcttt atgctcttat tccattcttt
    14041 gccgttttat ttggagcaga tgttgtcaaa agcgtcttgg ataatattcc agaggtcatc
    14101 atgaacggat taacagtcgc tggaaatctg ctgccggcag ttgggatggc gatgctgtta
    14161 cggatgcttt ggagtaaaaa tattgctgtg tggtttttct ttggggccgt tgttatggcg
    14221 tatctcgagt taccattgat tgcaattgcc gcagttggaa cgattattgc aattgttata
    14281 gcccaaagag atctacaact aaaaaatata tctgttcatg cagttcaaag cgatgctgct
    14341 gtaaatgcaa cgcttgacca agaagaggag gatttctttc aatgaaatta gctgatggaa
    14401 tttctaaaga tgagaaaaaa ttggttcgga agatgttttg gcgctcgatg actatgtatg
    14461 cttcggtgaa tccggtgacg atgggcggcg gtggtttcgc ctattctatg atgcccttca
    14521 ttaatcattt ctacaaaagt gaagaagatc gagctgcggc gctggaacgt catacacagt
    14581 atttttcgac gaccattcct tgcgcgtcct ttgtcatggg gattgcggca tcgatggaga
    14641 aggaaaattc tgaaaaagct ggttttgacg tggaaagtat caacgcgatc aaaacgagtt
    14701 tgatgggacc attggctgga attggcgatt cattgttctg gggagtttgg cgtgtcgtta
    14761 ccgcgggact agcgatcaac ttagccaatg ccggaaatat tttggcacca attatctttt
    14821 tagttatgtt caatatacct aattacttga tgcgatacta tggcggcgtt ttaggctatt
    14881 cattaggctc taaatatatt gaaaagctgt atgcgaacgg gttaattgaa attctgacaa
    14941 aagctgctag tatcttgggt ctgatcatgg taggtgcaat gacgatcagt atggtcaaat
    15001 tcaataccgt tttgagcttt tcaatgaagg gcgaagaact aatgaatgtc caagaaatcc
    15061 tcgatagtat ttttaaagga attgtaccgt tattggtaac attgagctgt ttcaaattac
    15121 tagataaaaa agtcggcgtc atcaaaatta tttttggaat ggtcgtcttg ggaattttat
    15181 tgtcattact tggaatcgca tagtaggaag ctgcagctac taggctgcag tttttttagc
    15241 aaggagggaa aagatgacta ttaatgaagt aatttattca cccaaacacc cattttttaa
    15301 tgctgagtta aacgacggaa atgtgactac ttcaaacgaa tacagctact atcggaatat
    15361 tcctgatgta aagggattga ccttcgatgt aagtaatcaa aagacattag caaaccttga
    15421 tgtttgcggg acgattcaaa cgttgacctt ttatcgagaa aatttactaa ctgaggaaaa
    15481 accaggtgtt tgggtcaata aacagctctc tcaaaccaat tcgttatcct taaaggttga
    15541 agtaaacggg attgtctacc atttgtcaga atcggatcat cgtattgaag tcgatgtgat
    15601 tcaagactgt ttgcctagat atattcatca ttattcgacg tttaaggtca ttgtagtatc
    15661 ctttgctccc atactggcta ataagcgttt aagtatgctt gttcaacagg tttacattgt
    15721 gaatgaaaca cacgaaagcc ttcaagtttc agtgaaagat gtgccttcgt atcaagagaa
    15781 gtattcggat cagcaaaatg tgttgatttc tcaaaaagga aataaggaca cgatcgcaca
    15841 acaggaagtg gcaagcttcg ccattgcttt tgtggaccca aatgcttttg aagaagtaat
    15901 gacttttcag gaatctgata tcgaaaaatg gctgaaggaa acattgaact attttgctca
    15961 gctatttggg cagctgtcaa tgccaaacga tagagttgtc catctctatc gtcgtgcact
    16021 ttatcaaagc ttttctagtt tcggtatgaa tcgacaggga caggtcgtcg gcagcaattg
    16081 gggcagctat cctgctacta atcgaatttg gaataaggat atgtattacg catcattgcc
    16141 attcaccatg tttgaaccgg agttatgtca aaaaacggtt ctatggtttg atcaatatgg
    16201 cgttaagttt cctggttcaa aatttacagg aggtatcaat cattctctga gtaattcgtt
    16261 atccagtgcg ttactggcta gtctttattt tgaacatact ggggatacaa aattctttga
    16321 gcggtgtccg caggttctac tgaatgcttc aagaattatt gaggacatat tagctcaacg
    16381 tcggtcaggg gaaccaatgc tttttccatc tgtttggtta tcagacgcct ttgcacttgg
    16441 aaagtatcat accggcagca atctttgtct ttggaaggcc tgtattggac ttgcagaaat
    16501 ttttgaagcc ctagaacaac tgagtcttag caagcgatac aaaaacattg cctgtaaact
    16561 aaaggaatca atttgtaaac aaatgactat cgatggtcct tttggtgagc aatttttgga
    16621 gggaatcggt gatgaggaga aaacaaccta ttcagtgaag cactatcaaa aaccgattct
    16681 tgagcaaggg ttaatctttt taagtgatgt gattgtggat ggaaaaatta atttattgat
    16741 gcatgatgga gaagaatcag acactacgtt gatgcctttt tatcaatttt tagataataa
    16801 agatcattta tatcagaaca ccatggcctt ttctgctagt tctttcaatc caacctatag
    16861 cgaggaaatc aaaggcatca cttggggatt agaatccggc gccacttttc ctggctttat
    16921 cactgtttta atgagcgagc tgcacaaccc tcaagctttc gtaacccgat tagaagaact
    16981 aatttgtttg gcagatttag acggttcgtg gtggtggtgg ccgtatcgct tagaggctca
    17041 acaaggagaa gtcgtgcgag actttggctg cggcaaatgc ggctgggcat cgggtctttt
    17101 tgtcagccta atgatcagtc aatatttcgg attgaaattc aaaaaaggag tattgcaaat
    17161 tgatcctgtt gataatttaa cgttttcttg gaagaatttg aagttcggtc aagccttcat
    17221 ttcgattgaa tgtacgcaat ccgagttgag tatttcaaat ttgtcagaaa agccattgaa
    17281 aataagtgat acgaaaaaaa ttcttcacgt agaaaaggga aaaacgattc acattcaaag
    17341 gagaaaaaga tgagtatact aaaaaatatt acccaattta aaaaaatcca gagtcgagca
    17401 atcactcccg aaaatccaac aggcgagaaa ggtaaagctg cgatgttgcc aagcgagcta
    17461 ggaccaagcc gcaaaggtcg tggcgcaatt ccgctgactt ctggtgaaga aactgtgatt
    17521 gcggatatta ctggaacggg aataattcgt catatgtgga tgacgattcg tgatcgaaca
    17581 gaatttggca gttttgtttt acgtgattta atcttaagaa tttattggga tgggtcggat
    17641 agccctgctg ttgaaagtcc tttaggggat ttcttttgta atgggtttgg agagcgttgc
    17701 gaggtgaact cgttgccgat agtggttaat cctacagggg gctttaattc ctatttcgaa
    17761 atgcctttcc aaacatccgc taaaattacg atcgagaatt tacatccgaa ggatatgtcc
    17821 tcattttttt atacgataaa ttacgctttg gtggacgaat tggaagaaaa tagcctatat
    17881 tttcacgcaa attggcatcg ggaacgcatt accaaattac aggaggatta taagttaatt
    17941 gatcgattaa agggtcaagg ctattacgtg ggtacttacc ttgcgcttac ctgcctagaa
    18001 cgttattggt ggggcgaagg cgagtttaaa ttctacattg atggggatag tgagtatccg
    18061 accgtaacct caacaggttc agaagattat tttggcgggg cgtgggcctt tcatgagtac
    18121 gatgagttcg gacgtccaca tgcgaaaaac tattcaacag cctttttagg gtatccttac
    18181 caatcaaata gagatgccac aagagatcgt tttgaaacgg gaaaattgaa tgccgttcat
    18241 gcctttggtg acgacgcaat gccacgacat ggtctttatc gttggcatct tcaagacccc
    18301 atctgtttta ccgaagattt atcggtcact ttacaacaaa tcggcaacga tgatgttcgc
    18361 ttatttgagc ggcaagacga tgttgctagt gtcgcttact ggtattcaac agaagcttca
    18421 ggaaatacta aacgtcattt cactgatcga gaacgcttgc cgcgttaatg aagtgttttt
    18481 cacatattcg aagggaggaa cctatgatga tatatggggc aattgaagct ggagggacta
    18541 aatttgtctg tgcagtcggt actgaggaat taacgatttt ggaaagagtc agttttccca
    18601 caacaactcc agttgaaaca atgaagcagg tcattgattt ttttaaacct tttaaggaac
    18661 aattggcagg catcggggtc ggctgctttg gtccaattga tattcaatgc aaatcaacga
    18721 gtttcggtca tatcacatca acaccaaaac ttgcttggca aaatttcaat tttgtaggaa
    18781 cgctaaagca atatttcaat attccgattg aatggacaac agatgtcaat gcggcttgct
    18841 atggtgaata tgttgctggt tctggcaaag ggttagcagg tgttgcatac ttcactgtgg
    18901 gaacaggtat cggaggtggt gccttgttca atgggacttt cgtggaaggg ttcagtcatc
    18961 cggaaatggg gcatatgttg atcaagcgac atccaaagga caatttttca ggaaactgtc
    19021 ccttccacca agattgttta gaggggatgg cagcaggacc agcgattgaa aaacggttgg
    19081 gtgtaaaagg ccagaattta ttggctgacg attcgttttg gcagattgaa gccttttacc
    19141 ttgcccaatg tgcatacaat acaacactga tgttttcgcc agatagaatt atttttggcg
    19201 gtggtgtaat gaaacaagaa cacatgaaga aaaaagttca ggataaattt gttgagctta
    19261 taaatggata tgttgaaata ccaccaattg atagctacat tattacgccc gaattaggtg
    19321 ataatgccgg aataatcggc ggattagcat tggccagaaa agcggtaaga aataagcaac
    19381 cataattaat gaatgtttca ttgatactca tgtcaaaagt atttaaccga gtaatcaaat
    19441 gatcaaatga agttggataa agcgaaaatg aattcaaata aaaaggctct aagtcaaatc
    19501 gtgttgtttt cattaaatca ctgaaaggaa atgcttctgc gtttcctttt ttctttttgt
    19561 tgtgcgctgt ataccttgag gatattcact ggtggcgtac acttaaatag taatatattg
    19621 tgttacttta tgtctgtggt aatagttata gttgcttacc aatgtatgtt catttttcga
    19681 aatgttgact tcataatcct tggcttaatg tttgaatttt tttggtgata gattttttca
    19741 gccgtataaa ataaatcgta attctattat agagagttgt gattataaaa agatagcaag
    19801 aaaaaaaaag aaagtttcta tacattttaa atattttatt cctatttcta ttgctaaaaa
    19861 agtagaaatt ctatatttac ctttaactct cgcttctctt gcaaatgtag ttagcatttt
    19921 aatcgataat aaactcaggt agttatatta ccttgaaatg attgataatt ttgtcacttc
    19981 tatttttttt gcaataaatt ttttatttta gtgaaaaata agaaactatt tctttgaagg
    20041 cgataagtta gaaaaatatt attgtagtct tttctttatt aggaatcatc actgttgtat
    20101 cttattacct tttctgtttt tcttattaga gaaaaataat ttgagaggta gatagttttt
    20161 attggaggaa gtaattatgt atatttttga aacaaaaatt tttcattgca ataattgcaa
    20221 caaagaaatt tcatcggggg aagaggtttg gacaaaaatg gaaatttccg ccaaaagtaa
    20281 ctgctcctca actgaaatca attaaagaac tagaatttga gaatgcactg gtaatttgta
    20341 tgacatgtac tgatgaagta tttcgaaaac atttttaaag gttgagatgt atatagattt
    20401 aaccaatcat ttatctctct cggaaaagga atattaacta accagcgggt tatcaaaaat
    20461 aggtaggaaa actgtactta atggcttatt tcgcgctgat tatgagaata tcagtctgtt
    20521 tgattaattt ttagggttca aaatacagag acaaactcca ggtcgggttt cgccacaagc
    20581 gaattacccg atctggagtt ttgtctattg caggaaggcg tagccggact gcaggaataa
    20641 agcccccggt t
//
LOCUS       DACECJ010000049        20484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.049, whole genome shotgun sequence.
ACCESSION   DACECJ010000049 DACECJ010000000
VERSION     DACECJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.049"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="IWO09_001801"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="IWO09_001801"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0264392.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="IWO09_001802"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="IWO09_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0264393.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="IWO09_001803"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="IWO09_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0264394.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="IWO09_001804"
     CDS             2856..3731
                     /locus_tag="IWO09_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0264395.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWO09_001805"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWO09_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0264396.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="IWO09_001806"
     CDS             4628..5062
                     /locus_tag="IWO09_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0264397.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="IWO09_001807"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="IWO09_001807"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0264398.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="IWO09_001808"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="IWO09_001808"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0264399.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="IWO09_001809"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="IWO09_001809"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0264400.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="IWO09_001810"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="IWO09_001810"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0264401.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="IWO09_001811"
     CDS             13333..14259
                     /locus_tag="IWO09_001811"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0264402.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="IWO09_001812"
     CDS             14441..15334
                     /locus_tag="IWO09_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0264403.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="IWO09_001813"
     CDS             15468..15593
                     /locus_tag="IWO09_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0264404.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="IWO09_001814"
     CDS             complement(15727..16359)
                     /locus_tag="IWO09_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264405.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="IWO09_001815"
     CDS             complement(16507..17049)
                     /locus_tag="IWO09_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0264406.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="IWO09_001816"
     CDS             17184..17573
                     /locus_tag="IWO09_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264407.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="IWO09_001817"
     CDS             17577..18341
                     /locus_tag="IWO09_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0264408.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="IWO09_001818"
     CDS             18470..19417
                     /locus_tag="IWO09_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0264409.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="IWO09_001819"
     CDS             19547..20287
                     /locus_tag="IWO09_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264410.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7192 a         3481 c         4316 g         5495 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
    20341 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
    20401 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
    20461 cgcagatcgt ttgcaaaaat agtt
//
LOCUS       DACECJ010000050        20118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.050, whole genome shotgun sequence.
ACCESSION   DACECJ010000050 DACECJ010000000
VERSION     DACECJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.050"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..497)
                     /locus_tag="IWO09_001820"
     CDS             complement(<1..497)
                     /locus_tag="IWO09_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0264411.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            603..1544
                     /locus_tag="IWO09_001821"
                     /pseudo
     CDS             603..1544
                     /locus_tag="IWO09_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
     gene            complement(1582..3693)
                     /locus_tag="IWO09_001822"
     CDS             complement(1582..3693)
                     /locus_tag="IWO09_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0264412.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(3724..3972)
                     /locus_tag="IWO09_001823"
     CDS             complement(3724..3972)
                     /locus_tag="IWO09_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264413.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            complement(3982..4809)
                     /locus_tag="IWO09_001824"
     CDS             complement(3982..4809)
                     /locus_tag="IWO09_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264414.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            complement(4905..5561)
                     /locus_tag="IWO09_001825"
     CDS             complement(4905..5561)
                     /locus_tag="IWO09_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0264415.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            complement(5574..7550)
                     /locus_tag="IWO09_001826"
     CDS             complement(5574..7550)
                     /locus_tag="IWO09_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0264416.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(7603..8274)
                     /locus_tag="IWO09_001827"
     CDS             complement(7603..8274)
                     /locus_tag="IWO09_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0264417.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(8360..8599)
                     /locus_tag="IWO09_001828"
     CDS             complement(8360..8599)
                     /locus_tag="IWO09_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264418.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            complement(8666..8905)
                     /locus_tag="IWO09_001829"
     CDS             complement(8666..8905)
                     /locus_tag="IWO09_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264419.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(9019..9360)
                     /locus_tag="IWO09_001830"
     CDS             complement(9019..9360)
                     /locus_tag="IWO09_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0264420.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(9347..9676)
                     /locus_tag="IWO09_001831"
     CDS             complement(9347..9676)
                     /locus_tag="IWO09_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264421.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(10267..10599)
                     /locus_tag="IWO09_001832"
     CDS             complement(10267..10599)
                     /locus_tag="IWO09_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0264422.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(11435..12475)
                     /locus_tag="IWO09_001833"
     CDS             complement(11435..12475)
                     /locus_tag="IWO09_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ0264423.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(12824..13150)
                     /locus_tag="IWO09_001834"
     CDS             complement(12824..13150)
                     /locus_tag="IWO09_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264424.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(13137..13925)
                     /locus_tag="IWO09_001835"
     CDS             complement(13137..13925)
                     /locus_tag="IWO09_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0264425.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(14293..14583)
                     /locus_tag="IWO09_001836"
     CDS             complement(14293..14583)
                     /locus_tag="IWO09_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0264426.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(14719..14898)
                     /locus_tag="IWO09_001837"
     CDS             complement(14719..14898)
                     /locus_tag="IWO09_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264427.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(14895..15245)
                     /locus_tag="IWO09_001838"
     CDS             complement(14895..15245)
                     /locus_tag="IWO09_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264428.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(15238..16563)
                     /locus_tag="IWO09_001839"
     CDS             complement(15238..16563)
                     /locus_tag="IWO09_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0264429.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            complement(16934..17518)
                     /locus_tag="IWO09_001840"
     CDS             complement(16934..17518)
                     /locus_tag="IWO09_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0264430.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(17729..17932)
                     /locus_tag="IWO09_001841"
     CDS             complement(17729..17932)
                     /locus_tag="IWO09_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264431.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(17942..18364)
                     /locus_tag="IWO09_001842"
     CDS             complement(17942..18364)
                     /locus_tag="IWO09_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264432.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            18678..19292
                     /locus_tag="IWO09_001843"
     CDS             18678..19292
                     /locus_tag="IWO09_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0264433.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(19316..19666)
                     /locus_tag="IWO09_001844"
     CDS             complement(19316..19666)
                     /locus_tag="IWO09_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0264434.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(19660..19923)
                     /locus_tag="IWO09_001845"
     CDS             complement(19660..19923)
                     /locus_tag="IWO09_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ0264435.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT         5997 a         3853 c         2988 g         7280 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctccaaaac aatcacaggt aatacaacaa tcacattcaa ttataatcaa gtgcatcaaa
      661 tcaatttgtt gtgcaataac aactacaaaa atgaggggcg agttgatatt ttgaaagaac
      721 agataatcaa cgtcactcac ggtgaaagtg tcactttaac tgctccttct attcaaggat
      781 atgttttaga cgatcgggta gaaccaacaa attcagtaac cttaaacaat gtgactgaaa
      841 gtcaaaatta tattttcaac tacactcgta aatttaatgt gactgttaat catgtaaatg
      901 ctgataccaa cgctgttctc tctagtgaat caaaaacagt atacgaaggg gatagcttct
      961 ctactccttg gaaaaatatg actgatcaaa attatttctt gtgcagaaat gacgatccta
     1021 ccgttaccgt ggataaaaac ggccaacgat ccattaataa cgtcgatcaa aataaaacaa
     1081 tcacattcaa atataaaaag atttcattag acgaattacg taactttatg cgtcaaaaag
     1141 aacttgcttg gttaaatgat tatcgccaac aaaatggtgt tgccccaatg caattcaatg
     1201 atattgttca acaggctgca gatattcgag ctaaagaatt acaagtcagc ttcagtcact
     1261 atcgccctgg tggtggtact ttccaagatt tattggaaag cttaggctgc tatggtgcta
     1321 agggagaaaa cataagtcaa actgggctgt atattgatga ccttttatca gacgatggtg
     1381 ctatcgatgc catggtagga tggaaaaata gccctgccca taatgcaaat ttactttata
     1441 ataaccaaag tattctcgga ttaggtcata attttgtagt tgactctgct ggtagaattg
     1501 gcgtagataa tgtatttatt agtgcgaatc caatcttcaa ataaaagaaa acaaggctga
     1561 aaatcagcct tgtttttata tttatttgag ttttaaaact acatagccca atgatgctaa
     1621 agtaagaaat aatatacttc caataacagt tagcactaaa tgattcgatc cagtttttgg
     1681 cacaatgacc tgaggtaagt taggttttac cccaatcgtt actacattag ttgctttctc
     1741 aatactttct actgaaactt ttgcttggtt gtcgatatcc aattcttcat tcaaaggagt
     1801 tttagctgta aatatcagtt gaatttctcg tccatctaaa agattgttat ttaagaaatc
     1861 agcggaagct tcccatttca gcgttcttga atggctatca taggttagta taccttctga
     1921 agtaatatct tttcctgtac tcatatctat cactcgcgca gaaccttctt tatacgtcaa
     1981 acgagcttcc actggatctt gaatactaaa ttgcttgtaa tgatcgagcg caatgtctac
     2041 accttttgta tgccattttt gtgtgactag atattgtact tcagatcctg catttaaagg
     2101 agctgtaggc gttccttttt taccatcatt tcgaccatca acatctttcc catcttgttc
     2161 cactttttta tttggtttaa gagagaaata gttttctact ttatttgttt tttgtgttcc
     2221 tttgttcgga tcattgttaa acgttacagt agccacattt ggtgcaattc cttttgcatc
     2281 attttttgct ctcatatgga gctgcaaaat gtagtcccta ccgtaatttg cagcattagc
     2341 taaaaagctg tcagaagcag tataagtgac tgtattacct gatacagtta attttcctcc
     2401 tgtatcagct tttttccaag tcccattcgc ttcaacatac cacaatgcgc catcatagtt
     2461 tttcccatcc gataccatat caaaattttt gctgtcaaag gtatcagtga ttttaaatga
     2521 tttgtatttg ttcattgtgt tgactgttaa tgcatcaaac ttttggtgaa tatgatagta
     2581 aacatctgat ccttgtggaa tcactttttt gtcaatattg tgtgctgcct cttcagtatt
     2641 agctttgtta aacacttctt ttgtaggctt cgatggttgt ggtgtccaaa gcaatgcagt
     2701 atcaaagcta aacatcatac tattcgtatt tgcgccaatc atgaatgttg gggaagcgcc
     2761 ttgaagcgta tacgttacac cacttctgtg ataagtatca gctcctacaa catcggtgaa
     2821 atgatcattc gagctataag ccttttctgc acgtcctccc actacttttg aagttaaatt
     2881 gtttgttgat aacgcaggag ctttgtattc tgcacatgct gtatctgttg tgacaaaata
     2941 aggtaagcga tccatttttt cgtacccaac aaattctctc atatcaatat tacgttcacc
     3001 gacagattca ttattgtcat tataccaaga cacacgttgc tgatactgtc catttaaact
     3061 gtaaatagat agaaatgtat cattcaactc aataccagct gctctcatcg atctcatttt
     3121 tgaattaatt tctggctgtg gttcactcaa agctactttt gtttttgatc ctgcatactt
     3181 aaaatctagt ttaacattca taacgttgat tgactgcatg aaaaagcctc gataaatgtt
     3241 tttatcaaag agcaaataat tataatactt tttcccatta attgttttag ctaatccgtc
     3301 ttggttaacc ccagcgtgcg ctacaaaatt agaaaaagtt acgtcgcaat caatttcttt
     3361 tcctagataa gtgcccacct tttgataatg ggcaacaatt tttgacgtgc ttttcaaatc
     3421 tttgactttt ggccaagcaa gtactttttg ttttttagtt gaatactcat ctagtatctt
     3481 aaatccattg atcccagtta cttcgatatt tctcaatgaa tcactatcat gataaagtac
     3541 cagattatta ttttctttga tttgactcgg atcaactttt tctaaggcct ggactgcagt
     3601 tccccttgca aaaagaagaa ggaacaaaaa caacactaaa gtaaatactc ttcccgtagt
     3661 gcaatgaatt ttattttcca taatgccctc catctattat tttgttaaac gattgtaagc
     3721 ttctcattct ttttttccta atacccacgt taccaatcct gcaagaagaa acaaactagc
     3781 cataaaaatt ggattgatta agagcttgtt aatcacttct agtggcatgg aagcatgctg
     3841 gatatttaga aaatgaacga acaatcgttg atatcttggg taaattgata attgagaaaa
     3901 aactaaaccg caacacaata ttgttgcggc taaaaaagat agattaaaaa ctaacatttt
     3961 tattattttc atcatttcat cctaatcgtt aaaaaataaa tgagtgaaaa aatcccaaca
     4021 attatcaaaa aagtttttct ttacaccagt tgatacaact tttttaattt cttctttcgg
     4081 ctctgatttc tttgatttag gatataacca aacatccgtc aaaaattcac ccttttcatc
     4141 tgaaacaggt gtcaataaaa caatatcttc gctatttgaa atgttttcag ggcttgattc
     4201 attgacgata tagtatgctt gttttttgtt tgcaggtact tcaattgaaa aatcaatcac
     4261 gccttcctga tcatcaattg tttgggtttc tccttcagtc acttttttca actgatcgta
     4321 tgaaagtttt tttgctcgat ctttcaattt tgactcaatt atttctaccg attgattttt
     4381 ctcattatca tcttgtgtca tttcttctaa gatattcgtg atatcgtaga caataaactt
     4441 tgcaccattg acaccttctt gctgagtcac ttgccttcct tgattgttga ttcgattcga
     4501 agaaataatt ttttgaatat gaatgttttg cttgattact tgcgtatctt ctacttcttg
     4561 cagtttttct gaagtctctg aagaactttc tgacgttgat tccgctgatt cagtagaact
     4621 cgtacttgaa tctgccgttt cttctgcagt gttcttcgaa ctgtccgtgt cacttgttgc
     4681 agttgttgaa ctatctgttg tctgactggt agttggagta gtactgctat cagcaaaaga
     4741 tatctgtgag taagctgcag ttgcaaagag agctgctaca caaacaaaca gccattttct
     4801 cttattcatt ccgatctcct ccttattttt ttataaataa aaataatagg aacgcaaaaa
     4861 aagcgaatag cagtgctaat cgccaatcca atgaaattga tttcctattt ttttgctttt
     4921 ttctgtagtt ttaaatcttc tttactgaat gacactcgtt caccatacac taaaaagcgg
     4981 tgtgagttaa caccttttgg cgtgcatgtc attaacgtga tcctatcttg gtctttttca
     5041 ggcatgagat aatctaaata atttttttgt aattcttctg ctttaacttc ttcaattcga
     5101 taaatttgat aggcatgttg ttcttcattt accttaataa agaatttttg cccttttttt
     5161 actgaaggca aatcatcgaa aagattatct ttataaagtc cactgtgccc cgcgattaaa
     5221 ggatttttac cgtttcctcc tgtaatatcg ccagttcctt ccgtaatccc tacaccattc
     5281 tctaaaattt tgtcggaagt tccgtcataa attggtagtt ttaaaggaat acttggaatt
     5341 gtaagaacac caacaggatc gcctaactct attttcgcaa catccaaaga agaatctgtt
     5401 ttttcaagat tttcaatggc taaaattctt tgctgttgtt gaaatttcgc tacatttgtt
     5461 tccacagctg gaattaaata gactgctact gccgacagca accaaaaaaa tatcatcagt
     5521 ttgtgcatga ctcttctttt tgggggctta cgaataatca ttagctgatc cttctaggct
     5581 ttcttcttaa agtaaacaac tgcagaaagt gcccctgctc ctagcaacaa gcaagagatc
     5641 aatacatacg tttggaaacc attactacct gttaatggaa tagctgcttt ttgattattt
     5701 ttaacagtga ttcgattttc ctctttaaat gagtttttgt ttactttaaa tgcaatatct
     5761 tctgttaatt tttggtaacc agttggtgct tttgtttctt ccagtgaata ttcaccttca
     5821 gttaaaccag tgatttcaaa gtgaccttct ttatctgatt ttaggatcgc tgcatttgct
     5881 acatcgtcta cccatactgc ttctttgaca ccgtctttat ccgtatcaac taatccacca
     5941 taatattttc cttctgcgtt tttagcagca aattctgcgc cttcaagcgg tgttgctttg
     6001 attcctgttc caaatagtcc gctatcgact tttaagaatt ttgcaccata agtcacaacg
     6061 tcagctttcg ttttttcgtg gtgttcttcc tgattaaatg gattgttgtt ataaacgaaa
     6121 tccacgctat ttgttaatgg attggcaaca gctgcgctgt catttacata catgttataa
     6181 gtcatttgaa gatcttgtcc gtgtaaactt gctacagtat cacttacttg gccattattc
     6241 acgataaaat cgatcacaaa gccattttct gaattttgga ttttataatc tgtattttct
     6301 tttaatacca cagatccatc tttatttgtt actttgatat ttgctttagc agcgacataa
     6361 gaaagtcctt gtcctgctgt atctccccac ttaaatacgc tgtaaggaga agtttcactc
     6421 ttcacaccat ttactgtaat cccatctgtt ccacctgcaa tatctgtagg gatatgaatc
     6481 gttccttgat aattcactgc gtcaccaatt tggaaagaat gttcttgtcc tgttccattt
     6541 gtcactgttt tatcgatcac tggtgcttta tagttcacgt aggaagcttt taagtcactt
     6601 gtcacgccgt cattggtaat ttcaaaggtt aatttattat gcgcatcgtt gcgagcgtct
     6661 gcgcctaata aatattgatc tgctgttggt tcttgatctg atcccactac aacttccgaa
     6721 ggaacttcca caaaatagta ttttcctaaa gtcaaatcag tcgcagtcag ctttccttga
     6781 tcatccgata caagatcacc caattttgtt ccttttccag gttcgccctt gtataactca
     6841 aattttgccc cttttaaaag ggtttgtttg ctttgggctg cgccatcgtc accaaatttc
     6901 gttaattcaa aactcaactt agaaacaatg ttcttaggat acaaatgaat attttttaaa
     6961 aatcctgatc ctgttttatc tgtcattggc gcaatcacaa ccattggttt tgctttttgt
     7021 gtgactaatc cagcagcgga tttactttca taaaccacat atacattccc tttggaattg
     7081 acataggctg gttgatcagt aaaagtgatc tcaccatttt catctacagc tgaagtaatt
     7141 ttgttggttg cattcttgac gtactcactg ttagatccct tgtcttctac atcttgtaca
     7201 atctcgtcca ctttcgcgtt ggttagatca gaatcttctc tcggtaatac ttgatccgta
     7261 atatttccta acgtaaactc aacatcacca taatctgcct tgttgtattt cgttacacct
     7321 gtaggatatt gatcgtgcgt ataaccatcg tttttgatcc cgtcaatatc aacattcaat
     7381 tgcgttcctt gatcgtacat taatttatga accgtgatat tgaccttctc tggtggtgtt
     7441 ttttgcgtga catcttctgc gtgtacttga tacccctgaa agcagcttgc aatgattcct
     7501 aacattccaa caagcattcc agcttttttt acataccgat ttattgccat tttttactct
     7561 cctcatttca tatttcaacg actcgcgttg attaaaacct tgttattgta gctctccctg
     7621 taccacgtat ctttcttttc cttgtgacgt ataaacacaa gtaaacaacg ttaataaggg
     7681 tttctctcct ttctttggta aagacaagaa agagttctct tcaggaacgc catcgattaa
     7741 tttaaagtac tcaggtacga taaatttacg cgttaccttg taacgataga cgccatcgtt
     7801 actaactaag tccatcacct cccctaatga caacttttga atatcagaaa ataaaacacc
     7861 tggcatttcc atgttatgac ctgctaggac atagtttcct ttgcccattt cggcatcttc
     7921 ataaaaataa gtagccgctc caagactcaa agtgtattga tttgcccctt taaaaattgg
     7981 caatgctaaa ccaagtgcag gtatctgaat ttgtgcaaag gcttgtttat tgacacccat
     8041 ttcgattgct tctttttgta tggacaacat ttgccccaaa gacatatcat tattgacgtt
     8101 cgttgcatcc tccttataac tctttgcatg ttcattgatt tgatctacgg gcatcttttt
     8161 ttctatattt tgcttaaaca cctttccttc atgtttttgg tagctaacat agccaaacac
     8221 actgcaaaag acgattaaag cgactcctaa aaaaccaaat aaccattttc tcatccagct
     8281 accccctttc agacaaaaca aaaaatcttt ttctcatttc catagaaaaa gttttaaaaa
     8341 tgaattctat taatgaatgt catgagactt ctaacaatag ttgacaatta gaactttttt
     8401 tcacataagt tcgtctcaac tgttcaaagt ttttcattga acctaacaaa caagctttct
     8461 cttttcctgt tatctgcata tcattgatat attgcatgac agataatctt ttaaggtgtt
     8521 tttgtttcat cgtttttagc aacttttctt ccatttcttt cgacactttt tttctgtttt
     8581 cgatttcttc ataattcatg agttttccta ccttccttgt tttttgcaaa aataaaaaag
     8641 cctatgatca catacgctct acacttcagt cttgggttat ctttttcttt cgtttaatta
     8701 ctggccgtgc tggtttcaaa tccttgattc cattatgggt tctccaataa tcgaattctt
     8761 ttcgtaatct ttctttttcc atatctgttc ctttagacag ggatttgaat acttctttag
     8821 gtgaataacc acgagctaac agctgtacat accttccttt tgtcaaagta tccatatcca
     8881 tcgcctctaa aactggtcgc gccatctaat tttcctccta aataaattcc ttactcaatg
     8941 cctaaggtaa tactaccttt ttattaatcg tcgtgactcc ttctaagcct ttatctgtaa
     9001 aatttattcc tccagttttt attcttcttt atccgctaca aattcaatga tttcaacgct
     9061 gacaatataa tcactcaaat atgaccaagt atcttcaaca gataattcgt ctccattatc
     9121 attcataagc gtcattttaa accattcatt accttgatca tcttcaggta gcaagccgaa
     9181 tccaattgga ttatcagtca atttttgatc gtataaatca tcctcgtcta atgctaaaac
     9241 atctaatcct ttacaatttc cgcctcttct aaacgtcaca aatcctttgt aatcccaacg
     9301 ttggaaagaa accttaatcg tatgtgttcc ccatcggcta ttcggatcat aatttttcat
     9361 ttgttgtaac ctcctcataa tcagttagta ctataaaggt atctttgttt ttatccaaaa
     9421 aatctaataa atcttttgta gaagatatct cttcatcaac ttcaccataa taaatacctt
     9481 cgataattgt tgctatatct gaaaaactaa tttttactgg acatttatcg tctaccgcat
     9541 tcaataccga caaaattgct tctttttctg caatctctcg tttgaatttc tttggagcta
     9601 atcgcatgac actcaagtca ttttcaagct tttcaatcca tgcagtttta ctcattttcg
     9661 caaatttttg aatcattcct ctaccacctt tgctatctac taaattagtt ttacttcata
     9721 acgaatattt ttatcgtttt tcgttcactc aaaaatatat aaaataaatc gactcgttta
     9781 tggcgtccga aacgagaaac cctagtagaa aacggtaaat gctcgattga tccaaagatc
     9841 aacgtcttcg caagtatcta catgtactgc agcacacgac acaatttctt gtgcgtcctc
     9901 cccattacga gaagacctac tccttattcc atgagtttgc gtcaaagcct agatccctta
     9961 gtagctgctc atgattaatc taggcgctat tctaacttgt ctaacttcta aattccctct
    10021 tgtttactct gtgcatatta tttacacaaa ttcatttcac cactatccaa acaagggcat
    10081 ttactcgatt gaccaatttt ttacgttacc aatcgtaaaa acctaccccg tcaccccccg
    10141 aaaggaattg ttgatagatc aagtctactc cttatttttc tagtgattct aagttccttt
    10201 agaagtctcg acaatatttt caagcaagcg aaaaacgata aaatacaagt tatgagaatg
    10261 atcaattcag ctgattaaac gccccatccc ttttgcggta caacgcaaga ctccattttt
    10321 tcttcgacca atcagtacac cgttttggtc ataaacatgt ttgattgttg gatatagctc
    10381 ttttaaaggg ataggatctt ttaaaattat ttgtttttgt ttcccaatgt aataaatcat
    10441 ttcttctgag actttgtctt tctggattat atatttctta cactgcacaa cggttttact
    10501 ttgaccagtt gatagtttct tcgtgatctt ttctgtagcg gtacaccgtt taccgtagtg
    10561 acaaatatct tcgagactca aaattcctgt atatgacata aaataatcca cccacttact
    10621 ttaaatattt ttaacaagtt tttcttgtta tttttcaaaa atagagaatt gattttcgtt
    10681 catgacaaac aaaaaagagg gaacgcatcc aaacatgcgt tccctctaat ttcagcgttc
    10741 gtcatacggc aaagagccac acggacattt ttataacatt atgcttaggc atagattcat
    10801 cgtaatatca cgtattttta accttaacac agccaaaaaa agtggtatca cttttgaata
    10861 tctgttatac taaatccgtt acgggtaata ccgtaacact gaaaggtaaa cgcattaagg
    10921 gagtgatctt aatgccacct gtggacgtcg acgaaggtta ccagccttcc gagtctcaca
    10981 gggcctttct tttttttgta ttttcaattg tccaaattta tcgtccaaaa gtgcagaacg
    11041 aaaatcaatt catcacaagc atagcagaca attaaattta aaacaactaa aaatatacaa
    11101 aaaagtgtaa ttgaatatta gcagcactgc ctgaaaagaa gtaatatcag gcttttcagc
    11161 ttggggaaat aagtgaatta cctctcttca tgaattatta taactcaaaa aagcacatat
    11221 gcaaacacta gttccctatt tttttgagtg aaaaataact aaaatatcta acgattatga
    11281 atttaaaacc tacgattgtg attgttttca taaactttta atctaaaaac tccggaatac
    11341 taactattta tttgtaatat ttgtaataaa attaaaatca taaaaaagtt ctaatttaaa
    11401 attgatcatt tttatagtaa ttgcttttta tcaactactc taaccaattt tccgtactaa
    11461 ctgttggtaa ttcgttaaat gtttctcttt gttcgtttgc aagctttgcc gcaatagctt
    11521 tttcgacaaa ccaatttttt acataaacga aaattagatt ctgcaaattg tttacttttt
    11581 cagttttttg tttttgataa gctttcagta aagtcatata taatcctttg tcagcatcaa
    11641 ttccataaga ttccagttct tcataaacga caatagtttg ctctaattct tcagcttttt
    11701 tctttgcatt atgaatagtt tggacggttt gttgagcttc actgtaactt gaagaaaatg
    11761 tttttatcaa gtttaataca ttttctggta caaatgtatt tatactgtcc tcttgcatga
    11821 tgtcgacgaa gttatctagc aaaacttgat tttgtaactg gtcagtttca gtgtctatta
    11881 tgtgtctatt agtgtctata gaattagaat agaatagatt gtctgcgatt ttcgcaatta
    11941 cggttgagtc cttgatatga ctggattttg ccgtatttgc gattttcgca attacggtag
    12001 aatcattgat ttttcgcggt tttgccgtat ttgcgatttt cgcaattacg gtggaatcag
    12061 tattgtcttc ctcttccatg attttaaaga cgtcttgatt cgtaacttct ggcttgccta
    12121 agtacaataa attaggcata gattcaatct taccatcacg tttatttact cttcctctct
    12181 tctgtttcag aagacctgct ttttctaatt cttttttgat cttagatact ttaccttcgc
    12241 ggcattgtaa caatttcatt aattcttcaa ctgtgaatat gaagaatata ttatcatcag
    12301 aatctaccca gttatttttg actgaataat caaatcgatc tttaagtagc atataagcaa
    12361 tttttgcatc atttgaaaga tccatgtatt tttcgttagt aaaaaatacc ttaggcattt
    12421 gataatattt ttcacgataa atttcttttg atttgtaata attaaaatca ttcatagcca
    12481 atcctctttc tgtaggatag cgttttgttc aaaactatgt tacaatgaat taaacaaata
    12541 tatctatcca ctatatattt ttctactgct gataagtcgc caaactttgt taaagcagta
    12601 gtcttttttt atcttttttt agtttacctt aaaaaataaa ttaaagcaaa ccgctctatt
    12661 tcagggttta agctcatggt aactatatca cgatattaca ataacattat atcacgatat
    12721 cgtaatatta caatatgaag ataagcgact tatcagcttt gatactactg atttttataa
    12781 aaaaaatatc acgatattat gatatcgtga tattgaatgt aaattaaatt ttattttgtt
    12841 tctttgctag tttatatcct tcaatcaagt attcttcagg tatggatttt aatagcgcat
    12901 cttctataac gtctggttta cttttgtctg attcagcaat agtcgtccaa tatttgagtg
    12961 ctgacattag aattggattt atacgaatgc tatctgctgt tcttacacta cttccttgat
    13021 cttttatgac tttacttata tttttcttgg ttaattcgat ttcttctact tcttttggtt
    13081 tgtagttctt tttattttta tttcctagta gtctacttac tgcagcttga ctgcgattat
    13141 cttctgccat caaaatcacc aacttctttt aaatattctt ctgtaaatgc tttatagtca
    13201 tcttcagctg ttgagggttt attaaaattg attagatctg atccagcagc ttgcgcttcg
    13261 tcaacggtta ttgttctacg aatataagtt ttgaaaattt ttgcaccaat gactctagaa
    13321 atttcggttg ctgcagtcgt catttcttta gaaatgttat aacgcttatt atacttagta
    13381 aataggactc ctcgtacttc aagcttgtcg ttcgtatatt cttttaccgt ttcaatagtt
    13441 tcacttaact ttattagtcc ttttaatgag aaatacgcag cttcaactgg aatgataatt
    13501 tcatcagaaa cagtaaatgc gttgactgta agcattccta aacttggtgg acaatcaatt
    13561 ataatataat catagttgtc cttgattggt tgaagtactc ttttaagcct gtgttcactt
    13621 cctacttgag taaattccct ctctgcagaa gaaagggcta tgtcggctgg caaaacatca
    13681 attcctttat agtccagaat agcatctgta atgtcagcct ccccttttaa tacttcataa
    13741 attgtaggtt ggtttgttcc atctatatcg aaacctacat tatctgtagc atttccctga
    13801 ggatcaatgt ccactaaaag tacattttta ccgtaaacaa atttcaaaca tgctgctaaa
    13861 ttaacgctag tcgtagtttt agaaactcca cctttttgat tggctactga caatattctt
    13921 gccattaaaa tatcccccat tcatcttata ttataatata attatatcat aatattgtga
    13981 tattgcaata tcgtatatgc attatattgt aatattgtgt gattattttg ttttaaagat
    14041 atatatcggt tcggatataa agattattca tatgttattc gaaagtaaaa taatcacttt
    14101 ttattacttt aaaatttttt attaatcata agaaatatgt tttgcgagca gcaactagaa
    14161 taatttaaac attattaaac tgtctatatt gttactaata attcccttct tatagtgcat
    14221 tttcttagaa aaaaacaata ttacgatatc atgatattaa gatatcgtaa tattatacaa
    14281 ttgtgattga tattatatat gtttttttat ttttttatca tgtttaatct tgaaggaatc
    14341 atattctttt tctaaagaca ggagcttttc ataaacatca gacatgttta aactagaggc
    14401 caggcttaaa tcataaacga atgaagcagg taatgatttt attctacgtt gtcgggttat
    14461 ccagcttgca accgttcctt gtgggtaggc atataactca caaaattgtt ctacagtgat
    14521 tcctaaattc ttgataatgt aaacattgat cgggtgcgag taaacaaatg tattgataga
    14581 catggtgcgt aagcctactt tcttttatta ttgcgtttgc aataatagtt taacacacgc
    14641 attattctag tatctactcc tatttgtaaa aacaaaaaag gattcataga gataactcta
    14701 tagaatccgt aaaatacctt atatatataa cgggaaaata taaaacctaa aattatgata
    14761 acctgctgca gcttctacat agaaagagaa gcgctcaaca ccaacctcat ttatcaaacg
    14821 ttggccataa ccccaaatct cttctgatag ctgatcaaag gttatatact ttagctcata
    14881 ttgctggata atgttcattc taatcgactc catttattat cctggtaaaa ttcgatatga
    14941 cgtatttcgt cgtagtgaat tttgtctttt ccaacgtaga tccccagctc atcactacct
    15001 aagatcttgc caacaatatc ttctggatag tatccttcag cgtttcgttc ttctttttga
    15061 atagatactg atctattttt tatgactgca gcgttcaata cttccgcaat ctcttcagtc
    15121 gtcatttgtt cttttgctaa attttgactg gttcgttgtt cattgtcggc atcaatttct
    15181 tcagtatgat cagataaata aaaccctgcc cactttaaac gtccacgatc attgtaatca
    15241 atcattttta attcccccat ttccgcccgc atgaccacca acaagtgtgc ttctagtaat
    15301 gcttctagcg ctttctaaca ttgaagaagc atgtacaatc gacgtaaaac catatttttg
    15361 ccgaatcttg tcaataattt tatctaaatt ttcttcgtta atttgctttt gtggatcact
    15421 aaataaatcc agctgaagcg aatcggtgta cacaagcttg ctataggtta ttccaacgtg
    15481 ccttacttct tgtccttcat aatacttatc aaacaagaaa agacaataat tctgcaattc
    15541 ctttgtatta tctgtgatag gtattttcat ctggtgtgaa aatcctggtc tcgtttctaa
    15601 gatcgacgta ccaatgttta aatgaacaca tcctgtttta caattatgct gcctgatacg
    15661 aatggctact tgttccgcca tttcttttac caccagctcg atttcatttc ttctagtata
    15721 atctcgtggt aacacttgtg aattaccata agatttttcc tttgtcggtt ctggcgggat
    15781 tgctatatct gttcgatcga ttccgttagc atggaagtac agctgtaatc caatcacacc
    15841 taatctattc ttaatcgtat aaggattcca gttagcaagg tctctaatgc tcaaaattcc
    15901 catttggttg agcctttttt tcattcgaga accaattccc caaaagtccg tcatttcggg
    15961 aatattccat actttctcag gaacgttttc gtacgtccat tccgcaataa atccttcatt
    16021 atgctttgct tcattgtcta aggctaattt agccaaaagg ggattatcgc cgacgcctac
    16081 cgtagcaatc aaacctaact cttctttaat acgttcttgg atcatttgag cgagcttctt
    16141 ccttcgctga cttcgtgttt cttcagtcgt aaaaagattc agtgattttg tcacttttaa
    16201 aatcgattca tcgatcgagt aaatcagtag atcttcatca gccacatatc ttctgaaaat
    16261 attatttacc tgcatatttc gcttgatata taggttcata cgtggtggaa caacgtgtag
    16321 tgttttagga aatggttgtg gtaagtcacg tggtctactc acatttgtaa taccataccg
    16381 cttttttgct tcaggagaag aagctaatat caatcctgaa ccagtattat cagatcgact
    16441 cataacaaca agttctgttg ttaatggatc taaatttctt tctatacatt cgacacttgc
    16501 gtaaaagctt ttcatatcga ttaaaaagta atcatttact ggctcttttg aataatccat
    16561 cataaataac actccattct tccacattat acaaacatat gttcgatgtt gtaaagaaga
    16621 aatagctaaa tacataaaaa aagaagcata ggtatgctat caccctgctt cttcttatga
    16681 tttgtatgat caatttattc tatcacttta ctaattaatt tgaaaatcta ataaaaactc
    16741 tcactctaaa agagtgagag actgcaaatt agagtacgga cttttttgga gttccgttct
    16801 ttaatattaa caaatgaata aaatccttac aacatttgat aataagttat cagtagacac
    16861 ttacaaaaaa ttctcatact attgtcactt ttctagataa atggcaaaaa ttttcagcat
    16921 taaatgacaa aaactaatga gaatttgtct ttccattttt ctcctccatt tttgccaaat
    16981 aacgataaat ggtggttctt gagacgttcc accgttccgc aatagtctta ataggtgtac
    17041 cgctatccac taaagtcttc agtaattcca aatcttgttc agacaatttt tcaggacgac
    17101 caccaaaccg tcctcttgca cgagcagctg ctcgtcctgc tgcggatcgt tccaggatca
    17161 aattacgctc aaattctgca aatgccgcaa acaaatgaaa catgagctgt cctgtagaac
    17221 ttgatttgtc catcgtaata ttttcttgaa tactatgaaa actcacacca cgctcattca
    17281 gtcgattgac gattgaaatc aaatcttcca tatttctacc taaacgatct aagcgccaaa
    17341 cgacaagtgt atcgccagag cgaacaaaat caatggccat ttctaatcct ggtcgattac
    17401 tttttgcccc actcatatga tcagtaaaaa ttttttcaca accaaattta tttaggctgt
    17461 cttcttgtaa atccaaattt tgtaaaccag tagatacccg ggcgtatcct attttcaaaa
    17521 aaatcactcc ttcattaatt ttattgtaac ataactttat ccatatagtt taactgaaca
    17581 tagatattgt tatgggttta tgaacatgaa atgaattgtt tttttgaaaa ataaagcaaa
    17641 aaaaggaagt gtccagaaat gggtgatttt atgaacactt tcaattagaa aaaaagaatg
    17701 tcgactagac actctttttt taaaatcatt atttttcttt ttgatatgtt ttttggtctc
    17761 ttttttttct tttttcttca gaatcttcta gattagaaat tgatttttga attttttgtg
    17821 tctcctgtaa agttcgctga acttgtaaag ctgattcttc tttcggtaaa ttaattttac
    17881 ttagaatatc gctatcttga taaaaatttg gtgtgttcac aaattctggc ataaaaaatc
    17941 cctactttct agttaaggtt cctgaataag tcgcaatcca attgtttcca gctgatttat
    18001 aactttgtaa atacaatttt ccatagtacc aataaccatt tcttttttct ttccaattcc
    18061 gtgattgagg aggtctagaa ggccaaccaa ctttatcaaa aatcatcgtt cctgtcactc
    18121 gtatcgtttt agaagctgct cgagcaagta atggagaaac tattaaataa ttttcagtaa
    18181 cagttaagtc gttagttggg ttaccaatgg ctattccgtt cctatctaaa ggagttaatt
    18241 cttggttatt atctactaca gtagttggtt catttgtact atcagcaaat gtaggaattg
    18301 tagttccagt tacactaatt ccaatagtta aaatacctaa agataaaata aacttatttt
    18361 tcattgtcta tcacctcgaa atcaaaaata agaaaacgtt ttcttaataa atcttaacag
    18421 ataaatcaag ttatatacta gaaaattcat aaaatatatt tttaatgtca taatcgttta
    18481 aattttcttg attgacattt atttacaaaa aatattgcca tttaaaaaga aaagtgacac
    18541 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
    18601 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
    18661 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
    18721 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
    18781 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
    18841 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
    18901 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
    18961 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
    19021 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
    19081 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
    19141 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
    19201 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
    19261 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
    19321 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
    19381 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
    19441 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
    19501 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
    19561 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
    19621 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
    19681 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
    19741 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
    19801 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
    19861 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
    19921 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
    19981 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
    20041 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
    20101 gaatcaggta agaaaaaa
//
LOCUS       DACECJ010000051        20004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.051, whole genome shotgun sequence.
ACCESSION   DACECJ010000051 DACECJ010000000
VERSION     DACECJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.051"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            438..992
                     /locus_tag="IWO09_001846"
     CDS             438..992
                     /locus_tag="IWO09_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0264436.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="IWO09_001847"
     CDS             complement(1165..1638)
                     /locus_tag="IWO09_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0264437.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2186
                     /locus_tag="IWO09_001848"
     CDS             1650..2186
                     /locus_tag="IWO09_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0264438.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2226..2597
                     /locus_tag="IWO09_001849"
     CDS             2226..2597
                     /locus_tag="IWO09_001849"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0264439.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2881..4152
                     /locus_tag="IWO09_001850"
     CDS             2881..4152
                     /locus_tag="IWO09_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ0264440.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4397..5422
                     /locus_tag="IWO09_001851"
     CDS             4397..5422
                     /locus_tag="IWO09_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0264441.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5717..6826
                     /locus_tag="IWO09_001852"
     CDS             5717..6826
                     /locus_tag="IWO09_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0264442.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6909..7805
                     /locus_tag="IWO09_001853"
                     /pseudo
     CDS             6909..7805
                     /locus_tag="IWO09_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7821..8645
                     /locus_tag="IWO09_001854"
     CDS             7821..8645
                     /locus_tag="IWO09_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0264443.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8677..9987
                     /locus_tag="IWO09_001855"
     CDS             8677..9987
                     /locus_tag="IWO09_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAQ0264444.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10135..11394
                     /locus_tag="IWO09_001856"
     CDS             10135..11394
                     /locus_tag="IWO09_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0264445.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11404..12276
                     /locus_tag="IWO09_001857"
     CDS             11404..12276
                     /locus_tag="IWO09_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0264446.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12288..13118
                     /locus_tag="IWO09_001858"
     CDS             12288..13118
                     /locus_tag="IWO09_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0264447.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13158..15341
                     /locus_tag="IWO09_001859"
     CDS             13158..15341
                     /locus_tag="IWO09_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0264448.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15402..16622
                     /locus_tag="IWO09_001860"
     CDS             15402..16622
                     /locus_tag="IWO09_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAQ0264449.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16619..18079
                     /gene="gtfA"
                     /locus_tag="IWO09_001861"
     CDS             16619..18079
                     /gene="gtfA"
                     /locus_tag="IWO09_001861"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAQ0264450.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18156..19262
                     /locus_tag="IWO09_001862"
                     /pseudo
     CDS             18156..19262
                     /locus_tag="IWO09_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19584..19793
                     /locus_tag="IWO09_001863"
     CDS             19584..19793
                     /locus_tag="IWO09_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0264451.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19786..19935
                     /locus_tag="IWO09_001864"
     CDS             19786..19935
                     /locus_tag="IWO09_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ0264452.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT         6839 a         3002 c         3880 g         6283 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt actttttttg
     5461 tttactaata tttttagtaa aaaatctcga caaaacatta agtaaacagt tatggtgttg
     5521 ttaacaaaaa gaaaacgcat tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat
     5581 agctttaaca gggatagctg ttttaactgg gacattactc gcagcatgtg gtgggggaga
     5641 tagtaattct aaagacgggc aagtaacttt aagctatgcg agttgagata ctgaccaagc
     5701 tccaggacta agaaaaatgt tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga
     5761 aatggagaca acgccttggg atcaatattg ggttaaatta gaagctgcaa caacgggagg
     5821 taatatgcca gatgtagtaa ccatgcactc tagcgaatcc tataaatata tgtcacagaa
     5881 tgctttgatg ggacttgatg atgttatcaa agaaaatgat attgacatgg gaaactttac
     5941 tgaaggaatt gctgatttct atacgcttga agacgaacta tacgcaatcc ctaaagatgc
     6001 ttctgtagtg gggctatggt acaacaaaga attatttgat aatgcaggtg tcgattatcc
     6061 agatgaaaca tggacatggg aaacactcag acaagctgca attgatttaa cagatgaatc
     6121 aaaaggcatc tatggttttg catcacatat tggaacagag actggctact ggccatttat
     6181 ttatcaaaat gggggagaag tatttgccga tgacaataca aaatcaggtt tgaatacccc
     6241 agaagcacaa gaagctttag ctttttatcg agatattgca cttgttgatc atgcttcacc
     6301 aacaattaca gaattacaag atcctgataa gttaagtcgt tttcaatcag gtagattagc
     6361 aatgatgatt gaaggaaact ggtttgcacc gggatttttg aaaaatgact atctagttga
     6421 aaatgttgct tctgccgtgt tacctaaggg aaaagttaat gcaacaatta caaatggact
     6481 tggttgggct gccagtgcga gtacaaaata cccagaagaa gtgaaaaaac taatggcatt
     6541 tttagctagt gaggacgcca ataaaatcca agcggaaacg ggggcatcaa tcccagcact
     6601 aaaaggcttt gaagacgagt gggctgcttc ttcagataca attgatttga gtgcttttgg
     6661 aaaaatgttg gagtatggag tccctcgtcc atacaataaa gatggattac gagctgaaca
     6721 agcagaagtt caaattatta accgtattct gtctggtgaa gtaactgttg aagatggaac
     6781 cgctcaaatt gaaaaagaag tcaatgcgat attgagcgga aattaaatta gttaattata
     6841 caatatacag gcgaaaggat tctatttcgt ctgtatatta atggatgaat accaaaaagg
     6901 gggatattat gaaaaatcga atttttggaa aatatcgtaa agatcaagtg atatggggct
     6961 atatttttat ttttccaacg gcattaggat taattttatt aaatatatgg ccagcaattc
     7021 aaacagcaat tttaagtttt caaaaaacgg tagggttcgg tgatactgaa tgggtcggat
     7081 taagaaatta ccaaaaaatg tttgcagacg gtgaagtttt tcagtcttta ttaaacacat
     7141 taatttatgc agtggtttcg atacctagta ttgttgtatt atccttgttg gtggcagttt
     7201 taatgaatag aaaagttaaa ggattgtcga tttatcggac aatttatttt ctaccagtcg
     7261 ttgctgcacc atccgcagtc gcaatgattt ggcgctggat gttcaataat gattatggac
     7321 tgattaataa catgttggct aaaataggtc tcgatggacc agcatggtta acggatccat
     7381 cgattgcgat ttattctatt atcattgtag gagtttggag tgctattggt tataacatgg
     7441 tgttattact agccggctta taagaaattc ctaaagatta ttatgaagca gcggaaattg
     7501 atggtgctag tccaatccag cagtttttca acattacgtt accattagtt acaccaacat
     7561 tattttttgt agttgtaaca acagttatca atgctttcca agtatttgat gttattttta
     7621 tgatgattac tcctaatagt accgccatgt ttaaatcaca atcattagcc tatttattct
     7681 ataaacattc atttatttta aatgacaagg gatatggttc ggcaatcgtt atgttccttt
     7741 tagtgattat attaattatt actgcaattc aaatgaagtt gcagaaaaag tgggtaaact
     7801 attaaggagg ctatcaaatt atgaaaaata aatttttttc aaatgcgcca atccatatca
     7861 tgcttatttt aggtggtatt gcaatggcat taccttttat ttggatgatt ttaacttcag
     7921 ggaaaacgtt ggttgaatct acccaaattc cacctacaat tttccctgag aaatttcagc
     7981 taagtaacta tcaacaagta cttgatttat taccattcat ggctttttat atcaatacat
     8041 ttttaatggt tgttggacga gtgattggct cggttttatt ttcagcaatg gctgcatatg
     8101 cttgtgcgcg tctaagtttt ccagggaaaa atatcctttt cagcttagta cttattcaaa
     8161 tgatgattcc cggttccatt tttattactc cacagtattt attagcacaa aaattgggta
     8221 ttttaaatac aattccagca ttaatattac caggggtagt tagtgcgttt ggtacgtttt
     8281 tattaagaca atttttctta ggtttacctg atgaattgga ggaagcggcc tatttagaag
     8341 gtgccaatat ttggacgatc tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg
     8401 ttgcacttgg aatattcaca gcgttgtttg catttaaaga tttaatgtgg ccattgattg
     8461 tcaatacttc aattgataag atgcctttag ctccgggatt agcaaactta caaggacaat
     8521 atgccacgaa cttcccacaa ttaatggcag gttcgttatt agcaatttgg ccaatagttg
     8581 tgatattcat cgttttccaa agaaaattca ttgaaggaat tgctacaaca ggtagtaaat
     8641 tataaaacta ttaattagtg attggagagt aagtacatga caaaaattac atttattggt
     8701 gcaggaagta ctatttttgc aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta
     8761 caagatgcac atattgcatt gtacgatatt gatgaaaaac gactaaaaga atcaaattta
     8821 atgcttaaaa atattaataa aaattcgaat gaaaatcgtg ctgatattgt ttcttattat
     8881 gatcgcaaag aagcgttaag agaggctgat tttgttatca atgctattca agtgggtggt
     8941 tataaacctt caacagtgat tgattttgat attcctaaaa aatatgggtt gcgacaaact
     9001 attggtgaca ccatggggat tggtgggatt tttagaaccc tacggacact accagtaatg
     9061 ttagactttg ctaaagaaat ggaagaagtt tgtccaaatg cttggttatt gaactataca
     9121 aatccgatgg cgatgttaac catgggtgtt ttaaaagcga cgagtattaa gactgttggt
     9181 ttatgtcata gcgttcaagt atgcgtacca gaattgtttg agcatttagg aatagccgat
     9241 caatatgact taaatgactt tcaatggaaa attgcaggca ttaatcatat ggcattcttg
     9301 ttggaaatta caaaaaatgg ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa
     9361 aaaccaaatc cacatccaga ttctgtacgt tttgaattgt tgaaacgttt tggttactat
     9421 attacagaat caagtgaaca caatgcagaa taccatccat actttattaa atcaaaatac
     9481 ccagaattga ttgacgaatt agggattccg ttggatgaat acttacgtcg ttgtgaacaa
     9541 caaattaatg attgggaaag catgcgagag gacattgtaa ataatgggga gttaacacat
     9601 acgagaagcc gtgaatatgc ttcatatatc atggattcta ttacgacagg aactccgtca
     9661 gtgattgcag gaaatgtctt aaataaaggc ttaatcacaa acttaccgac agatgtttgt
     9721 gtggaagtac cttgtttagt cgataaaaat ggcgtgcaac caacctatgt cggtgaattg
     9781 ccaacacaat tagctgcttt aaatcggacg aatatcaatg ttcaagaact gacagtagaa
     9841 gccgctttaa cactagataa atcaaaaatt tatcaagcag cgtatttaga tccacatcta
     9901 tcatctgaat tgtctttaac agatatcaaa aatttagtgg atgatttaat cgaagcacat
     9961 ggggactatt tacctgaata taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc
    10021 cttactcttt tcttagttaa atttagtaaa aatattgaca aaacacttag taaacagtta
    10081 cattagtgtt aacaaaaaga aaacgcattc aaattgtata aggaggagca taaaatgaag
    10141 tggtctaaaa aaattatttt agcttgtata gcactaggag caacgatctc tttgactgct
    10201 tgtggaaaaa atcaggaaga tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa
    10261 aaagaaatga ctcaaacgat tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat
    10321 attcatgtaa aagttgtgga tgtaccgaat gctggtgaag taattaaaac acgtatgctt
    10381 gcaggcgatg taccagatgt cattaatctt tatcctcaaa gtatagaatt gaaagaatgg
    10441 gcgaaagctg gttatttaga ggacttaacc gaagaaccgt atttagagaa tatcaaaaat
    10501 ggatatgcac aacgttttgc tattgaggat cgtgtatata gcataccatt aaccgctaat
    10561 gtctatggtt tttactataa caaaactgct tttgaggaaa tggggattca agcaccagaa
    10621 acttgggcag aatttgaaaa aatagtagct gagattaaag atcaaaacaa agtaccgttt
    10681 gcgattgcag gagcagaagg ctggacttta aatggttatc accaactagc tatagcgact
    10741 gctgctggtg gtgaagagga agcgaacaat gtttggcgtt tctcagaagt taatggaatt
    10801 aatgctgaaa gcaaagaaat gcaaaaagat tttaatcgat tagatttgtt aagagaacca
    10861 aaagctttgc aaaacaactg gcaaggtgcc ggttataacg atacagtagt tacttttaca
    10921 aaaggtgatg cgttgattat gccgaatggt tcatgggcaa tgccgatgat tcattcacaa
    10981 aatccagatt ttgaagtagg aacttttcct tttccagcag atgaagcagg tcaaagtcta
    11041 acgattggcg caggagacct agccttatca gtttctgcaa cttctcaaca taaagaagaa
    11101 gccaaacgtt ttgtagaata tatgacaaca cctgaagcga tgcagaaata ttatgatgta
    11161 gatggtgcac cgtgtgctgt tgagggcgtt atcgagaata ctcaggattc accattaagt
    11221 ggtttaactg aacttgcttt tactgatcgt catttagttt ggttagccaa agattggaat
    11281 agtgaaaatg atttttatac cttaacaaca aattatttac acaatggcaa tcaaaaagcc
    11341 atgattaacg cattaaatgc tttctttaat ccaatgaaag ctgacggaag gtaggaaaat
    11401 aaaatgaaaa taaaagaaat tttaaatcgt tactgggcaa tcatttttgt agcaattcca
    11461 attattttgc agatgatttt tttctacttt ccaatggttc aaggtgcttt ttacagttta
    11521 actaattgga ctggtctgac ttataatttt gattttgtgg gtttgaacaa ttataagatt
    11581 cttttttctg atccgaagtt tatcaaatcg attggcttca ctttaattgt gacgctttgc
    11641 atgattgctg gacaaattgg ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt
    11701 aaagggaaaa cattctttag agcatggttc ttttttccag cggtcttatc gggcgtggtt
    11761 gtttctttag tatttaaaca aatttttaac tatggtttac caaaaattgg tagtatgtta
    11821 ggcattgaat ttttacaaac cagtctttta ggaacaacat ggggagcgat ttttgcagta
    11881 atttttgttt tactttggca aggaattgcc atgccagtca tcattttctt agcgggtttt
    11941 caaagtattc cacaagaaat tattgaagct gctagaatgg atggggcgaa tgataagcaa
    12001 attttttgga atattgaaat cacctactta ttaccaagta tttcaatggt atttattatg
    12061 gcattaaaag gtggcttaac agcatttgat caaatttttg ctttaactgg tggtggacca
    12121 agcgatgcca caacatcttt ggggttactt gtttataatt atgcttttaa aaacaatcag
    12181 tttggttatg cgaatgctat tgctttagtc ttatttatct taattgggat tgtttcactg
    12241 gcacaattaa aattatcgaa aaaatttgaa atctaggagg tttcccaatg aaaacatcaa
    12301 aagaaaaaaa tatttctaaa tacttgcttt tattattagg gggcttcatg attttagttc
    12361 ccttagtttc aacgctattc ggttcattta aaacaacacc cgatattatg aataatttct
    12421 ttcgttttcc taatccagcg actttggaaa atttccgaag attgttagcg gatggaattg
    12481 gtcattactt ttggaattcg gcaatcatca caatcattgc agtagttgct gtgacagcaa
    12541 ttattcctat ggctgcttat tcactagcaa gaaaaatgac tcgtgggaaa gcatacggat
    12601 tgatgtattt atttttaatt ttaggaattt ttgttccttt ccaagtaatt atgattccga
    12661 ttaccgttat gatgagtaaa attggtttag caaatatgtg gggattaatc ttactctatc
    12721 tcacttatgc cgtaccacag acactatttt tatatgttgg ttttattaaa ctaaatgtgc
    12781 ctatcagtct ggatgaggct gctgaaattg atggggctag tcactttaca acttatttca
    12841 aaattgtttt cccaatgtta aaaccaatgc atgcgacgac tattattctt aatgctctat
    12901 ggttttggaa tgatttcatg ctaccattat taattttaaa tcgtgattct aatatgtgga
    12961 cactaccatt gtttcaatat aattatacgg gacaatattt taacgactat ggtccaagtt
    13021 ttgcttccta tacagtagga attgtcacga ttacactagt ttatcttgct ttccaaagaa
    13081 gtattatagc tggaatgagc aatggagcag tgaaataacc aataaattat tctcattata
    13141 aaggagttca taaacttatg attatatttg acaatcacac acaaacattt catttacaaa
    13201 ataaaaaaat tagctatcta ttaggaatcg aagaaaataa ttatttaacg catctttatt
    13261 ttggaaaaaa gattacgcac tattctggtg ggtatcgcta cccccgaaca gatcgttcct
    13321 tctcacccaa caatgcaaat gcgcaagatc gtttattatc tttagacaca ttaccattag
    13381 aatttccagg atatggacat ggtgatttta gagaaccggc gcataatatt aaattaggaa
    13441 acggtagtcg tgtaaatgat tttcgttatg atagctacga aattcaaaaa ggaaagccca
    13501 gcttagcggg attgccagcc ttatatacgg aagatgatga tgaagctgaa acgctgatta
    13561 ttcaattgaa agacgcgatt agtggattga ctttacagtt gttctacaca atttatcaaa
    13621 atcacagtgt aattattcgt tctacgcaat tagtaaataa tagtgatgaa acagtggaga
    13681 ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg tccactcgag ttaattcatt
    13741 taaatggtac ctgggcaaga gaacgtcaaa tgactagaga aaaaattcat acagggataa
    13801 aagtattaga tagtaaacgc ggatcaagca gccatcaaca aaatccgttt gttgctttag
    13861 tagatgccaa aacagatgaa tttcaaggag aggcatatgg atttaattta gtttatagcg
    13921 gtaatcacga aacggtgatt caaaaagatc catttgacca aacacgggtc attatgggaa
    13981 ttaattcatt taattttggt tggcaactaa aaactggcga ctcatttcaa acgccagaag
    14041 tcgtgatggt ttatagtaat gaaggtctga atacgttatc acagacgtat catgaattgt
    14101 ataatcaccg tttaattcgt ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca
    14161 actgggaagc aacgtatttt gattttaacg aagaaaaaat catgaatatt gtcgatgatg
    14221 cgaagaactt gggcattgaa atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa
    14281 atgacttcac ttcattaggt gattgggttg aaacagaagg caaacttgct tgtggtttgg
    14341 aaaatttagc caagcaagtg catgataacg ggatgaaatt tgggatttgg tttgaaccgg
    14401 aaatgatttc tgaagatagt gaactatttc gagcacatcc agattgggca ttgggtattc
    14461 caggtcgtga ccgctcaatt agtcgaaatc aatatgttct tgatttttca agacaagatg
    14521 ttcgtgataa tatctatcaa caaatgacgg cgatttttga tcgtgtaccg attgattatg
    14581 tgaagtggga tatgaatcga aatatgacag aagtctattc tgtcttgttg gactcaacga
    14641 tgcaaggtga agtggctcat cgttatatgt tagggttata tgaattttta gaaaagttaa
    14701 caacgaatta tccaactatt ttatttgaaa gttgttcggg tggaggcggt cgttttgatg
    14761 caggtttctt gtattacatg ccacaaactt ggacgagcga caataccgat gccgtggctc
    14821 gtctgaaaat tcaatatgga accagcttag tgtatccaat ttcaagtatg ggtgcgcatg
    14881 tttcagcagt cccaaatcat caaacaggaa gagaaacgag tttaactatt cgtggcaatg
    14941 ctgcgatggc tggtgttttt ggttatgagc tagatctaac gcaattaact tctgaagaaa
    15001 aagcagaaat taaagaacaa gttgcctttt ataaaaagca tcgccaactt ttgcaatatg
    15061 gaacttttta tcgcttggaa agtccgtttg aaggcaatga tactgcatgg atgtttgtct
    15121 caaaagacca aacagaagcc atggtctttt atttccgagt attagcagaa gctgcgcatc
    15181 cattagtcac attgaaatta gcaggattag cagaagaagc aacttatcaa atggaagatg
    15241 taacatttgc tggcgatgaa ttgatgaatc tcggttttta tattgactca gaactacatg
    15301 gtgattatgc gacacaacgc ttttatttga aacagatata gtagcttgaa tttacaatat
    15361 aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa tatgttttat ttacacgcat
    15421 ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg
    15481 caatgattaa ctattggtta gataaaggac taggcggatt tagaatcgat gcgatattaa
    15541 acatcaaaaa aaaactagag tatggcatct ttgaatctga tggagaagat gggttagcct
    15601 tcattgggcc ctggattctt aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag
    15661 agcggacatt taaaccacat aatagtatga cggtagcaga agccgatgtt cctaatgaac
    15721 gtttagatga atatattggt gaagatggct attattcgat ggtttttgat tttagttata
    15781 cggatattga tgtacctgaa actggtgaat ggtttaaaga tagccaatgg acatggacgg
    15841 atatgcgtac caatattttt acgaatcaat tagttacgca agataaaggt tggggcgcct
    15901 tatatttaga aaaccacgat caaccacgtt caattaataa atatattcca gaagaatgga
    15961 tcaatgatta tagtaaaaaa atgttggcga cgctttttat gttactaaga ggtacccctt
    16021 ttatttatca aggacaagaa ctaggaatga ccaacataga aatggcatca ttagaagatt
    16081 ttgatgatgt agctacgcat tctcagtata aacgggcatt agaatatgga ttggcaccag
    16141 agcaagcatt gaaagctgtc aataaacgaa gtcgggataa ttctcgcaca cctatgcaat
    16201 ggaatacgca aaaaaatgcc agattctcaa cttctgataa tgtatggtta aaagtaaatc
    16261 ctaattatcc agaactcaat gcagaagctc agttaaccaa tcaacagtcg gtattcaatt
    16321 attatcatca attaattaaa ttaagaaaaa atagtgtata caaagacgtg ctaatctacg
    16381 gacaatttct accagtgaaa gataaaaatg aacatatttt gctttatgaa cgccaattag
    16441 aagataaaat cgtattagtt atcttaaacg ttacagcaac agaacaagaa tattatgtag
    16501 atgaacagta ccaacaagta ttaatcaata attatgaaga tgttttatta gatcaaagtc
    16561 gaaaattgcg tttacgaccg tttgaaagta ttgtattagc taattatgga ggacaattat
    16621 gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat agtttaggaa aaaacatggg
    16681 cgaattaaaa caagtcttag acaaacattt acaaggtgtt attggtggtg tacatctgtt
    16741 gccttttttt ccatcgacag gtgatcgagg ctttgcgccg agtgattata cgacagttga
    16801 tccagctctt ggaacatggg aagatgtgga agcattaggt gaagattatt atttgatgtt
    16861 tgattttatg attaatcaca tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa
    16921 ccatgagcag tcaccataca aagaaatgtt tatccgtatt catgaatttt tcccagaaaa
    16981 tcgaccaaca caagaagata ttgatttaat ctacaagcgt aaagacaaag cgccgtttca
    17041 aacggtagaa tttgcggatg gtacgacaga agaggtttgg aatacctttg gggaagaaca
    17101 aattgattta gatgtgacca aagaagttgt gaaagaattt attcgagaaa ccatcaaaga
    17161 tatggcgaac catggctgtt ctttaattcg attagatgcg tttgcttatg cgattaaaaa
    17221 attagatacc aatgatttct tcgtggaacc agatatttgg gaacttttag acgaagtgcg
    17281 tcaagaagca gcgaaatatg acgtcgaatt attaccagaa atccatgagc attattccat
    17341 tcaaatgaaa attgccaatc atgattatta tatctatgac ttcgccttgc caatggtcac
    17401 tctgtattca ctttatagtg gaaaatcaga acgtttagcc aattggttga aaatgagtcc
    17461 gatgaagcag tttacaacat tggatacgca tgatggaatt ggtgttgtgg acgctcgtga
    17521 tttattaaca gatgaagaat tggattacac ctctgaggaa ttgtataaag tcggggcgaa
    17581 tgtgaagaag gtctattcaa gtgccaatta caataattta gatatttatc aaattaacag
    17641 cacctattat agtgcattag gcgataatga ccgtagctat ctgttagcac gtgcgattca
    17701 agtatttgcg ccagggattc cacaaattta ttacgttggg ttattagcag gaaaaaatga
    17761 tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt aatcgtcatt actatgattt
    17821 agccgaaatt gaagaagaag tgcaacggcc agtgattcaa caattatttg cacttttaaa
    17881 atttagaaat gaatctgcgg cgtttgattt ggatggaacg attgaagtag caacgccatc
    17941 agaatcagaa atagtgatta cacgtaaaaa taaaaacggt agtgatagtg caacattaac
    18001 ggctaacttg gatacgaaag aatttatcat tgagtctaaa gatataaaaa ttttcactag
    18061 tgttgcatta aaattttaac ctattccata gaacaaaaaa tcctttatac tagcacttgg
    18121 atgacttatc caagaccaat aaaaaggact catacatgga taagtataaa acaaatacag
    18181 catttaataa atggttttct gccataaaat taggaaaact ttctgtcaac tcccaaaaat
    18241 gtatttttga ttacaattcg tatcgtaaaa aactgtcttt tgaagctgtg ctcaagctct
    18301 ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga tttgagtaca gctcttatcg
    18361 accgatcgct ccaactagaa accgatgtgt catcgattag tcatacccaa ttgtctcgag
    18421 catttaatgc acttgaacct aaagtgttag aagaaatttt tcaacaactg ttagaaaaag
    18481 ttcaacatca aatcaaacca accaagcgaa atcatctgta tctgattgat tccagtacgt
    18541 tttcgcttag tttaaaacgt cataagtggg cgactttccg tcaaacaaaa tcaggtgtca
    18601 agttacactt aaactattgt tatatggacg atactacgat gtatccaact gattttacct
    18661 taacaaatgc ccatgaacat gatctcaatc aactaccagt attggtgaat caaccagagg
    18721 caacttacgt gtttgatcgt gggtattagg actttgaaaa gatggatcag atgcattggg
    18781 atggctattt cttcgtcact cggattaaaa aaaacaccaa agttcacgtg atagatactc
    18841 tggaaacatc gccagaaaca gaaattctac gagacgagct ggttcgttta ggatctaaaa
    18901 cctatctaac cgccaatttc cgactagtca ctgttcaaga taagaacgga cgtgtgtttc
    18961 aatttatcac caatcgaatg gacgtctctt caaaagaaat ctctgatatg tatcacgctc
    19021 gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca catgaccatt aaaaccttct
    19081 tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt aacgatgatt tccgctctcc
    19141 tcacgtttct catcaaattg gagacgaaaa cggaaaaatc agtgttccaa atcaaacgat
    19201 tctttcgtta tttactgttt cagccctttg aatgttgtct agaaaaatta attcccacat
    19261 gagaaaaaag aaatttggct cgttactttt ggttttcagt tgaataacag ataaaaaatt
    19321 ttctggaaaa agtcatcctc tgcgtcagca cagttttttt tgacttattt tcagaggaaa
    19381 tagttttcag aatattctaa aatgttttat gcaacactag tgatattttt tagaattaga
    19441 tttctaatgt gcattttttg agtattaagg agcaagtata caaagattta taaagctgat
    19501 taaatttgaa taaaaatatg caggaacgct actattttta gtagtgttct tttttatatt
    19561 tgaataaaaa atgtggtggt taaatggtgg ttataacaaa aaatgaatta atgaaattag
    19621 gttatgggaa gtatcagtct gagagaatca tacgacaggc aaaagcaatt atggtttcaa
    19681 aaagctatac ctattacgaa aatagaagat taggactagt acctgttgaa tctgtagaag
    19741 aagttttagg tatgaagata gaggtagagg aggattttca gaatcatggt taattttaaa
    19801 aaagataaaa atgggaaata tcattttact gctagtttgg gattcgacaa acgaacaggg
    19861 aaaagagtac aaaaaaacgt agaggggtta aaacagttaa agaagaaaaa aagcgtatac
    19921 cgaacttgtt tctaattatg gaaaagaagc agaaacttct caaagtacta tgttgtttga
    19981 ggagtttttt tataaattat tcct
//
LOCUS       DACECJ010000052        19480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.052, whole genome shotgun sequence.
ACCESSION   DACECJ010000052 DACECJ010000000
VERSION     DACECJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.052"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWO09_001865"
     CDS             133..3660
                     /locus_tag="IWO09_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0264453.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWO09_001866"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWO09_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0264454.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWO09_001867"
     CDS             7448..7789
                     /locus_tag="IWO09_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264455.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWO09_001868"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWO09_001868"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0264456.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWO09_001869"
     CDS             9146..11566
                     /locus_tag="IWO09_001869"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0264457.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO09_001870"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO09_001870"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0264458.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWO09_001871"
     CDS             13000..13737
                     /locus_tag="IWO09_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264459.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWO09_001872"
     CDS             13751..14566
                     /locus_tag="IWO09_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0264460.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWO09_001873"
     CDS             14579..15226
                     /locus_tag="IWO09_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0264461.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWO09_001874"
     CDS             15242..15898
                     /locus_tag="IWO09_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0264462.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWO09_001875"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWO09_001875"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0264463.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWO09_001876"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWO09_001876"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0264464.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWO09_001877"
     CDS             18240..18758
                     /locus_tag="IWO09_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0264465.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWO09_001878"
     CDS             18865..19365
                     /locus_tag="IWO09_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0264466.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3344 c         4182 g         5402 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DACECJ010000053        19473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.053, whole genome shotgun sequence.
ACCESSION   DACECJ010000053 DACECJ010000000
VERSION     DACECJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.053"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IWO09_001879"
     CDS             complement(21..995)
                     /locus_tag="IWO09_001879"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0264467.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IWO09_001880"
     CDS             complement(1075..1950)
                     /locus_tag="IWO09_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0264468.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IWO09_001881"
     CDS             2080..3987
                     /locus_tag="IWO09_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0264469.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IWO09_001882"
     CDS             complement(4528..7806)
                     /locus_tag="IWO09_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0264470.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="IWO09_001883"
     CDS             complement(8077..8622)
                     /locus_tag="IWO09_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0264471.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="IWO09_001884"
     CDS             complement(8634..9326)
                     /locus_tag="IWO09_001884"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0264472.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="IWO09_001885"
     CDS             complement(9345..9629)
                     /locus_tag="IWO09_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264473.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="IWO09_001886"
     CDS             complement(9642..10205)
                     /locus_tag="IWO09_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0264474.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="IWO09_001887"
     CDS             10433..11095
                     /locus_tag="IWO09_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0264475.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="IWO09_001888"
     CDS             11219..12415
                     /locus_tag="IWO09_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0264476.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="IWO09_001889"
     CDS             complement(12516..13892)
                     /locus_tag="IWO09_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0264477.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="IWO09_001890"
     CDS             complement(13929..14795)
                     /locus_tag="IWO09_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0264478.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="IWO09_001891"
     CDS             complement(14884..15909)
                     /locus_tag="IWO09_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0264479.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="IWO09_001892"
     CDS             complement(15927..16601)
                     /locus_tag="IWO09_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0264480.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="IWO09_001893"
     CDS             complement(16785..17678)
                     /locus_tag="IWO09_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0264481.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="IWO09_001894"
     CDS             complement(17752..19449)
                     /locus_tag="IWO09_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0264482.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4190 c         3445 g         6154 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 caacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt tta
//
LOCUS       DACECJ010000054        19402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.054, whole genome shotgun sequence.
ACCESSION   DACECJ010000054 DACECJ010000000
VERSION     DACECJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.054"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            315..1094
                     /locus_tag="IWO09_001895"
     CDS             315..1094
                     /locus_tag="IWO09_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264483.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1140..2084
                     /locus_tag="IWO09_001896"
     CDS             1140..2084
                     /locus_tag="IWO09_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0264484.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2100..2879
                     /locus_tag="IWO09_001897"
     CDS             2100..2879
                     /locus_tag="IWO09_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0264485.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2891..3589
                     /locus_tag="IWO09_001898"
     CDS             2891..3589
                     /locus_tag="IWO09_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0264486.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3617..4381
                     /locus_tag="IWO09_001899"
     CDS             3617..4381
                     /locus_tag="IWO09_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0264487.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4425..5876
                     /locus_tag="IWO09_001900"
     CDS             4425..5876
                     /locus_tag="IWO09_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0264488.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5908..7044
                     /locus_tag="IWO09_001901"
     CDS             5908..7044
                     /locus_tag="IWO09_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0264489.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7044..8150
                     /locus_tag="IWO09_001902"
     CDS             7044..8150
                     /locus_tag="IWO09_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0264490.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8156..9178
                     /locus_tag="IWO09_001903"
     CDS             8156..9178
                     /locus_tag="IWO09_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0264491.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9200..10255
                     /locus_tag="IWO09_001904"
     CDS             9200..10255
                     /locus_tag="IWO09_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0264492.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10873..12108
                     /locus_tag="IWO09_001905"
     CDS             10873..12108
                     /locus_tag="IWO09_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264493.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12192..13280
                     /locus_tag="IWO09_001906"
     CDS             12192..13280
                     /locus_tag="IWO09_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264494.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13277..14698
                     /locus_tag="IWO09_001907"
     CDS             13277..14698
                     /locus_tag="IWO09_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0264495.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            14791..15450
                     /locus_tag="IWO09_001908"
     CDS             14791..15450
                     /locus_tag="IWO09_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0264496.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            15469..17052
                     /locus_tag="IWO09_001909"
     CDS             15469..17052
                     /locus_tag="IWO09_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAQ0264497.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            17187..17765
                     /locus_tag="IWO09_001910"
     CDS             17187..17765
                     /locus_tag="IWO09_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264498.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            17711..18229
                     /locus_tag="IWO09_001911"
     CDS             17711..18229
                     /locus_tag="IWO09_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264499.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            18237..19277
                     /locus_tag="IWO09_001912"
     CDS             18237..19277
                     /locus_tag="IWO09_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ0264500.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT         7067 a         2435 c         3803 g         6097 t
ORIGIN      
        1 taactattat taattcaatg ttaatttttt tgttattcta ttttttgtgt aacttttaaa
       61 aacacctatg aaaattatgg aaataaaaat gaaatcgatt aatttttatc ttattaatgt
      121 tttatttact tttaaatcgt aaattgccct agtcaagaaa acagtttgtg gtatgattat
      181 atagggaaaa cttgtttaca aatatttctg tgaatatatt agtttaatta tgttttttta
      241 aataaaaagc tgtcattttt atgtaagtga attatatctt aatttatcta tcatcaatac
      301 ttaggagtga atctatggaa aagccattcg tcaagtcatt gactgctgtg gcggtaggtg
      361 caggtgctat tgcgatctgt ttgtttggat accatatcaa taatcagcgg caacatcagc
      421 aacggatcaa ttatgcagaa tctgccatca ccaatcaaaa agacaccttg acttctttat
      481 caaaagaagt agacaagctg tatagtacaa aagaaaaaat ttttctaaac cctgaaataa
      541 cagaagaaac aatcacaagt ttgtcaaata agctatcctc tgtcaaactt tcagcagatg
      601 attacgacat caaagaatca gaattgccta aagaagctgt tgcaatccaa gatgagaaga
      661 aagcaatcgt gacacagtta gatagtgcag aatcaaaact gaagatccag acagctgtca
      721 acgatttatt tactaaaaat gtatcaaact ggcagcaagc tgtagacgat gtcatcatca
      781 aagaaaaggt gacaagtgca gatgtcgctc gtgtacgtga aaatatgagc ttttttaaag
      841 atagtccttg gaaaacggtt gttatgcaat atctaggatt cgctgatatt cagatcgcgc
      901 aagtcacgca actagatcag ttattcgata cgatgttaaa ggatggtcaa gtgactgcca
      961 ccgctactta cgatcaatat ttgacggtgc tcagtcaaat agagcagatc cggaatgaaa
     1021 aaataagcgc agcatacgca acaaaagcac aaactgttgc tgagcaaatg ggctatagca
     1081 atataagcta ttgacagtta aaacgttgat tcaagcaagg ttagcaaacg gaggaaacta
     1141 tggcaagacg aagaagaaaa tcaagaggga aaaaaatatt tctgattatt ttagcagttc
     1201 ttatcgtatt agtattagca attgtaggag taggagcaaa attatatctg gatctctcac
     1261 attctattga gaaaacttac gaatcagtgg aacgaaccca aggagaacga acgaaaccag
     1321 ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat actggagatt
     1381 taggtcgggt cgatcaagga agatcagata cgatgatggt tgccacagtc agtcctgaag
     1441 acaaacagac aacgatcgtc agcattcctc gtgatacgta tgtcgacatc gtaggtcgtg
     1501 acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg gcgatggata
     1561 ccgtacaaaa atatgtagac atcccaatcg atcactatgt ctctatcaat atgaaagggc
     1621 taaaggaact cgttgatgca gttggtggta tcgaagtcaa taatgacctc actttctcac
     1681 aagatggcta tgactttcca atcggtaaaa ttacattgaa cggggatcag gcattatctt
     1741 attctcgtat gcgttatgaa gacccaaatg gtgattacgg tagacaaggg cgccaaagaa
     1801 agatcgtaga aggcgtagcg aaaaaagtat tgagtctaaa cggtgtcagc aactatcaat
     1861 ccgttttgaa tgctctagag tcgaatatga aaacagacat gaactttgat gaaatgaaaa
     1921 aaattgcttt agattatcgg gaggcatttg gtacgattaa gcaagatcag ctgcaaggag
     1981 aaggcttcat gcaagataat atctcctatc aaaaagtcag tgatcaagaa cttgatcgtg
     2041 tgaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt ggagaaaaaa
     2101 tggaagagac aatcagcttg aaagaaattt ttgaaacatt acgcaaacac ttggtaataa
     2161 tcatcatctc aatgtttgct ggcttggcca tctcaggaat cgtaacattt ttcatcttga
     2221 caccaaaata cagttcacaa gcacaattga tcgtgacact gccacaatca gacactgcaa
     2281 atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaagat atgatcgtca
     2341 gtgatttagt gctcaatgaa gtcaaagatc gtttagaaac agaagacaat gtgaaaatga
     2401 ctgctgaaca aatcaaagat gccatctcag ttaatcaggc agaaaattca caaatgttct
     2461 cgattcaggc aataagtacg aatgcagtgg ctgctcaaca gatcgctaac acaacttcac
     2521 aagttttcca agagaacgca aaagatgtga tgaatgttga taaaatctcg atcatttcag
     2581 gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaattta atgactggct
     2641 taatgttagg aatgatggta ggtgtaggtc tggcattctt attggaatta ctcgatcgga
     2701 cagtgaaaga tgaaaaatat gtgacagata cattaggatt cccaatctta ggaacagttc
     2761 cagaaatgtc agcgaaagaa ttgaatgctg gtatccgtaa aaaaggacca gctcagacac
     2821 aaccaaaagc tacaaacgat aaaacagcgc catcacgcag aagccgctca agagtctagg
     2881 aggaaagaaa atggcacgaa cacagaaaca aaaagcagcc caaactcatg cagtcagttt
     2941 ggtcacactc accaatccct cttcaccaat cgctgaacaa tatcgtacga ttcggacgaa
     3001 tatccagttt gcctccgcag ctggtcagca aatcaaaacg attgttgtga cttcatcagg
     3061 acctggagaa gggaaatcga ccactgcagc aaacattgcc gttgtttttg ccaaatctgg
     3121 tcaacgggtg ctgctagtcg atgcagactt acgtaagccc gtcatctata aaacgttcaa
     3181 gttgaacaac gcaagcggat taagtactgt tcttagttca tcaggaagtg tagcagatga
     3241 gatccaacga acaccagtcg acaatttgtc gattctgcca agtggaccta aaccaccaaa
     3301 tccatcagaa ctactaagtt cgccaagaat ggatcagatt ttagcagaag caagacaact
     3361 atttgatgta gtgatcttcg acatgccgcc agtcgttgcc gtaacagatg cacagatcat
     3421 gtcttcaaaa acggatggca ccttattggt cgtacgggaa aatacgtcac gaaaagaatc
     3481 attgactaaa gcaaaagaac tgttagatat ggtacaagca cgagtcttag gtgtcgtata
     3541 caacggagca gaacacagca aagatgctgg ttattactac ggaaattaag gaataagaag
     3601 agaggagaca agagaaatga tcgatctaca ttgccatatc ctaccaggaa tcgatgacgg
     3661 ggcagaaaac ttagaagcaa gtattgcaat ggctgaaaaa gcgattcagc aaggcattac
     3721 gcatatttta tgcacaccac accacaataa tggaaaatac agcaatgaaa aatcacaagt
     3781 catttctcta gtcgcttcgt tacaagccga attagagaaa agacagttgc ctttgacgtt
     3841 attggaagga caagaagtac gtattaccgg aacgctcatc gaagacatcc accgagatga
     3901 aattttattt acagatttag atgatacata tcttctgatc gaatttccga cactggaagt
     3961 gccgttatat gcagaacggc ttttttttga gttgttagaa ctaggaaaga taccagtgat
     4021 cgtccatcca gaacgcaacg cgcattttcg agaagaccca aatcatttgc ttccgttcct
     4081 agatatggga tgtttggccc aacttactgc gccaagttat gttggtgtat ttggcaaaga
     4141 tatccagaaa acagccaaaa caatggtcaa acataatctc gtgcagatgg ctgcttctga
     4201 tgcacacggg gtctctaagc gcacctttta cttaaaagag tgctatgagc agattgccaa
     4261 ggagtttggt aaggaaaaag tagtacagat gaaacaagtc gcaaaggatc tgatcaacgg
     4321 ggatgagatc aattatccaa tctatgaaga ggtaaaaaag aagaagtttg gtttatttta
     4381 gttctttatt cgtagataaa aaggaaatga ggagagagtg agagatgtat agaaaaaact
     4441 caagtggttg gtctaaacat attgatttta ttttattaga cttattttgt gtccagcttg
     4501 ctttttatat atcatatgta atgcgacagg gtgactggaa tccgtatgtg attcctattt
     4561 atcgccacat ggcaattttt atagaacttg agaatcttac gattatattt ttatttgagg
     4621 catataaaaa tgttttaaaa agaggatatt ataaagaggc tgtagcttct ataaagcaat
     4681 cttttatgct gttacttgtg tgcagtttat accttattac aatgcaggat ggaaatgatt
     4741 attccagagt ttctttattt ataatggggg tggtgtatgg attattaaca tatataattc
     4801 gtatactttg gaaagggttg ttgcatcacc gcatgaaaaa cggtgaagat gtatcgttag
     4861 ttattgtaac atctcaaaag attgcaaaaa atgtagttcg taatcttaaa gagaagaatt
     4921 atggcatgta taaacttagt ggtgtgattg tagtagacag agatatgtat ggaactacca
     4981 tcggaggtgt cccagtagtt gcaaatgctg ataatgcagc ggaatatctt ttacagaatt
     5041 gggtagatga agtattcatc aatgttgacg aaagtgttcc atatcccaaa gaattaatcg
     5101 ccagatgttc ggaaatggga cttgctgtac atatgaatct tacaaaagtg tctgatagta
     5161 cacgtggaaa acaatttatt ggccgagttg gtgattatac tgttattaca tctagtatta
     5221 atgttatgac gatgaggcaa gcatttataa agagaacgat tgacattctt gctggaatta
     5281 ttggatgtat tgcaacaggt gttattttta tcttcgtagc accagttatt tacatcaaat
     5341 caccagggcc gattttcttt gcacaagaac gtattggtca aaatggtaag acatttaaga
     5401 tgtataagtt ccgtagtatg tatatggatg cagaagaacg caaagcagag ttgatgaaag
     5461 aaaataagat gagtaataat ctcatgttta agatggattt tgacccaaga atcattggca
     5521 ataaaatact tcctgatgga acaaagaaga caggaattgg acaatttatt cgatctacat
     5581 cattggatga gtttccacaa ttttataatg tcctaatggg aaaaatgagc ctggtggggt
     5641 ttagaccttg cttgaagtca gaatatgaga agtatgattt tcatcatcgt gcgagaattg
     5701 cgatgaaacc tgggattact ggaatgtggc aggtgagtgg tagaagtgat atcacagact
     5761 tcgaagaggt tgtaaaactt gatgcagaat atatcaggaa ttggagtatg ggattagatt
     5821 ttagaatttt gtttaggaca gtcaaaactg tgcttcatag aaacggttcc atgtaagata
     5881 aagatactat agaaggagta aaacgtgatg gcaagaaaaa aattaaaaat ctgtatgcta
     5941 ggtcataaaa gaattccatc aagagaaggt ggaattgaga tagtggtaga agaattggca
     6001 acgcgaatgg ttgcacttgg gcatgaagtg acttgctata atcgaagtgg tcatcatgtt
     6061 agcggaaagg aatttgacca gcataaaaat aaagaatata aaggtgtaaa attaaagact
     6121 atttttacat taaatataaa aggaattgca gcaatgagtt cttcagtatt tggtggaatt
     6181 agagcagcat tcggaaaata tgatgttgtt cattttcatg cagaaggacc atgtgctatg
     6241 ttgtggttgc caaaactttt tgggaaaaga tgtgttgcaa caattcatgg tctagatcat
     6301 caaagagaaa aatggaacaa gcttgctagt acatacatca tgttagggga aaagtgtgct
     6361 gtaaaatttg cagatgaaat aattgtactt agtgaaagcg tccaaaatta ttttgaagat
     6421 atatatggaa gaaaaacaag gtttatacca aatggcgtta aaaaaattga aataaaatca
     6481 gcaggattga ttacagaaaa gtatggatta acaaaagatt cttatatttt gtttttgggg
     6541 cgattggttc ccgaaaaagg aattcggtat ctaattgagg catttaaaga tgttcagaca
     6601 gataaaaaac ttataatagc cggaggaagc tctgatacag atgaatttgc aaatgaacta
     6661 aaggaattag caaaaggtga tgagcgaatt atatttactg gatttgtaca aggacaggaa
     6721 ttagaagaat tatatagtaa tgcgtatatc tatacacttc caagtgattt agaaggaatg
     6781 ccattaagtt tgttagaagc aatgagttat gggaactgtt gtatagtttc aaatatctca
     6841 gagtgtacgg aagtagtaga agacaaggct atgattttta agaaatcgga tgtaagtgat
     6901 ttaaagataa gattagaaga ggcatgcaat caatcggaaa tggttaaagt tttgaagaat
     6961 caagctactg agtttatttg tagtaaatat aattgggata aaatcgtaca agaaacatta
     7021 aatttgtata ggggaaatat ataatgattc gaatattaca gtgtgtgaat gatatgcatc
     7081 gtgcagggtt ggagacgatg ctaatgaact attataggaa tatagatcga actaaaattc
     7141 agtttgattt tttgacacat cgtccatata aaagtgatta tgatgaggaa ataataagtc
     7201 ttggaggaaa ggtctattat gcaccgagac tttatccaca aaattatcct aaatatttta
     7261 aatggatgga acaatttttt aaggatcatc cagaatatga gattgttcat tctcatattg
     7321 atgcgatgag ttatttacct ttaaaagctg caaaaaaagc tgggattccg atacggatag
     7381 cacatagcca taatacatcg atagatagag attttaaata tcttttaaag caatattatc
     7441 gatgtaagtt aacgtctgtt ttgacacatg aattttcatg tggaaaagta gcaggtgatt
     7501 ttttgtttcg aaatgataaa ttcactttga ttccaaatgc agtggatgct aaaaaatttt
     7561 attatgatga aagtattaga aatgatgtac gaatgaaatt aggaatttca agtcagacgt
     7621 ttgtaatggg acatattgga agaatttcat attctaaaaa tcatcgtttt ttgattgaaa
     7681 tttttaaaga atttcacgct attaagaata attccgtatt attaattatt ggaactggtg
     7741 atatggaaga agaaataaaa aattatgcaa agaaaagtgg catagatgat gatattaaat
     7801 ttttaggaaa tagaaatgat atagaaaaat tttatcaagc atttgacgta ttaatgttgc
     7861 catcattatt tgaaggggtt ccgttagtgg gtattgaagc tcaatttgca gatttaccgt
     7921 gtttcttttc ggagaaagtt cctacagaag ttgctttcag tgataagacc aatttcataa
     7981 gtttaaatca atcggcaaaa gaatgggcga gagagattgc aaatgtagat atctctcatc
     8041 gagattcaga acgttcgatt ttaataaagg cagattataa tattcaaaca gcatacaaaa
     8101 tcttagaatc aaaatattat gagttatttg aattaataca aagaggttaa agaatatgac
     8161 acagaaacat tcaagtataa aaagaattat acatcgcata tccgaagaat tgaatgcaac
     8221 aagacgaatt gcaaagataa catcttggaa ggaagcattt gccacgttac aagctaaaat
     8281 tgatattcaa gtcatgaatc gtaatggata taatgaaagt aaaaaacaga aggaacattt
     8341 attaaaaaaa catgaggtaa tgctgaagta ttatgaaaag actttttcag attttttgaa
     8401 aacatatgat tatagtcaaa atcaaataat taatcaatca aaatcaccat attctaactg
     8461 catttgggtt tgttggtggc agggattgga tcaatcgccg gaaattgtaa aagcttgtgt
     8521 aaaatcaata aaacgaaatg cagggaaaca tactgttata attttaacag aagataatta
     8581 tagacaatat gttgatatac caaaatgggt ggaagaaaag aaaaacaaag gaatcattac
     8641 gagaacaaat tattctgatt tattaagatt atctttgcta gcaaaacatg gaggtttgtg
     8701 gttagacgcc acattttatt gtacaaaacc aataccagat gagtattttg atatcgatat
     8761 ttggtctatt aggaggcctg attatgctca cgcatccgta gcatcaggat attttgcggg
     8821 ttattcatta gcatgtaatc aagagtctag atggatgttt gctataatta gagacttttt
     8881 tttaaattat tggaaaaata atgatatgat ggtggattat ttgatgatag attatatgat
     8941 tgttttagca cagagaataa atcatgaaat agcacagaca tttgataaaa tacctagtaa
     9001 caatccatta tgtgacgaat tgattaaaaa tttaaataat atatatgatg aaaaagtatg
     9061 gaacttatta aagaaagata ctttactgtt taagttgaca tggaaacaat cttttaaaaa
     9121 gcaaaaagat gataaagtta ctttttatgg gatgttggta gaaaacaaat tattataaat
     9181 attagaggta taaaaagtta tgaaacacaa ggtaagtgtg atagtaccaa tttacaatgt
     9241 tgaaaaatat ttaagaaaat gtgtggatag tttattgagt cagacattaa aagagataga
     9301 aatcgtatta gttgatgatg gttcgccaga tgcttcgggg gaaatagcag atgagtatca
     9361 aaaaaaatat tctaatgtaa aaacaataca tagagaaaat ggggggttag gaccggcaag
     9421 aaatacagga attgagaatg cgacaggaga atatgtggct tttcttgatt cagatgattg
     9481 ggtagaaagt gatatgtatg aaaaattata cttagttgcg tccaaagaaa atgctgatat
     9541 agtagttagt ggacattgtg attttgcgaa tgacaaagct atggtgaaaa agcaacatcc
     9601 tttgagtggg aatgtatatg atacgcctaa tagtattaaa aatgtacgaa aaaatttatt
     9661 tggacatagt ccaaaagatc aagaagtaga ggccttcccg atgtcagtat gtatgtcttt
     9721 gtataataaa aatctgctag ataaatatag actaagattt gaaaaaattt tatctgaaga
     9781 tacaattttt aacttgaatg catatgatgt cgcaaataaa attgctttta cagaatatac
     9841 agattattgt tatagaaaag aagaacaaga gtctattaca aaaagtttat ctccaaataa
     9901 gcagacggaa tttaaagaat ttttaataac tctaaaagct atagcatcga aagaagattt
     9961 agaatgtcaa atcagagcaa aacgtatggc aattgattat tgtaggcttt atgtgggctt
    10021 agttgatgat gcaaatttgg gaattaaaga gaaaagaaag tatataaaaa aatttattga
    10081 agatgaggga ttaattcaat gttggaaggg atatcctaca aatacgctac catttcaaca
    10141 aaaatttttt cataaaatgc ttgaatgtaa gtgttataca atattgttgt tgttaagtag
    10201 aattagaaag ataattaaaa atatgaccag aaaaaaattg tttataatat tataaactaa
    10261 aagtatatca aataatagaa attaacaagt tacaaaaaaa tattataaag aataatattt
    10321 tctaaaaagt aaactatagt aatgtggatt atcaccttcc aacagtatat aaaatgagcg
    10381 taaaaacggc tatttttgcc ttaacaacga tttttttgta tacaaatacg atatgctcat
    10441 taattattgg aatttgtagt gctgtttaaa ttaatgaagg tgtaattata tgagtgatca
    10501 ttttgaaaaa gtttgttgag aagatgtgct tctagtcatg tgatgggaga aaataattta
    10561 actgttagag tgaatagtta aattattctg gttatatgaa aaaagtagtt tctaaaataa
    10621 tcttgcaggg attaacttaa agaaattgag agaatagttt ctttgaaatt tctatataca
    10681 atgagaagct gaaatttact tttatttgat gactggaaat agaaattaaa tatattgtat
    10741 tgttacatta aagaaaactt gtatggaaaa atcagtacaa taccattatt taatgttctt
    10801 cacttaaagc gcaaaaaata tcagtttatt aaactcgtat atagaacaaa tatatgtaga
    10861 cggggcgaaa cgatgagtgt aaaaaaaatg ctatttatgg taagtacaat aattgtatta
    10921 acatcccgat tttggggaat taatactagt ttatctatgc gatattttat tatggcaata
    10981 tgggatttat attttatgtt aagctatttt tcttatcgtg agaaaacata taaaaaatac
    11041 gttatcaaga ataacttttg gttgactata atgccgtttg tgatattttc tatctatact
    11101 ctaataatat ggggaattgg ttcaaatgca gaatttgaaa attttataaa gttatgttcc
    11161 acgttgcttt atttaattct tgcatatggt tacgcatgta cagcatttta cttatttaag
    11221 gcagaaggaa ttgattttat tttttggtca ggtgttattt catatatgag tggttcagtt
    11281 ttttatttaa ttgtatctta tggtttaaat ggtttaattt cttatataaa aagtcttata
    11341 acaggtgaaa caaatactgc aaattttgct atggaggtac atgatttaac ttttgcgatg
    11401 ggggtttttt tcctttatta cgtatttttt gaaaataaaa atacaaaaaa acataagata
    11461 aaaattatcg tatctttaat tttaattttc atgggattga agagaattga actgttagct
    11521 ttaagtggtg caattctagt gtattatgtt ttattaaagt ggggaaaaaa gattcaaatc
    11581 cgtgcattag tgtgtactat ttgtgcaact gttatttcgt tagtgtttat ttacattata
    11641 gatacaggaa ttttggcatt gcttatgtca aatttgggca ttacagatga cggtgcgagg
    11701 ctttcatttt atctatattc atctaaattt tatgaacttg gtttaggata tgtaggaaca
    11761 ggatggacat ggttttcaaa atactttttt aatctttatt tatcgcactt taggttaaat
    11821 ggtggaagaa ttgcagcaag cctacacagt gatatattaa ctatttatat agaagttgga
    11881 tgtatagttt ttattatatg gatgttttgg ttattttttg tgaaaccgat aagactaact
    11941 aaaaaatttg gattgaaaac aggggagtgt gtattattat taactatata tatgtttata
    12001 ttatatttga cggataatac gctcacttat ccagacactc aaatgatttt tctcttagta
    12061 cctatgatag tttcgtgggt tgataatcaa caacgaatat cggaatagat agctaaagta
    12121 tattttaatt atgaatttat aattagttgt aaaagttaat atttaacgga caggaagtag
    12181 ggtgaaaaaa tttgaaaaaa attttagtgt tagcaaatac atattatcaa ataatagtag
    12241 caatacaaat gaaaaataca attttttccg atgagtatgt tgaaattatt atttctgatc
    12301 attctaaaaa cactaaagaa attgcgaaaa aaatagaaga aaaaaaagtg tttgatggct
    12361 gtcattattt agaaagcaaa tcttttgatg ataatattgg gtattttcaa aagattaagg
    12421 actgtcttca gatttcttgt ggtaaatcaa atcgatatgc ctattatttg aaaaactgta
    12481 aaagtgaata ttttgatgaa tttatttgtt tttcaatggg agaggtttgg ttggaaggaa
    12541 tatatgcttg cttatatcga aataataaaa atgtacaggt atcaatttta gaagaaggta
    12601 ttttaagcta tggagttgac attattccaa cagcaagaac aaaagctatc gcagtactca
    12661 attacttaaa aaaaaggact ccactatata acaaaagcta taatttttat tgtttttttc
    12721 ctaatatata tacaggaaga gagaatacaa tacaaattcc attagttaca gctgggtcaa
    12781 gaaccgcatt gcaactttcg gatattttta accctatgat agatatttat aaagaaaaaa
    12841 aatatatatt ttttacgtct gtttatgatt ttgagggcgg agaacctgtc ggagaatatg
    12901 aattagtatg caaaatagca gacttagtag gaaaagataa tctaattgtt aagacgcatc
    12961 ctagagatgt aagaacaatt tataaagata atggatttat cgaggataaa aattcatcaa
    13021 taccttggga ggcgattcaa ttgtcaggcg attttaacga taaaatattt atgacaatta
    13081 attcaggaag tgttatttca ggtagtacaa tgtctgcaaa acctgtacgc acgtattata
    13141 tgtataaatt gtgtaacata gaaggaaatc cgtcttgcaa aaagaatgcg tatgatattg
    13201 agcgattgct taagcaaaaa acgatgaaaa acattttaaa gaatgtgaaa atagcagaaa
    13261 gcttagatga tatactatga gcaaaactaa atatttaatt aaaaatatgg gaatattaac
    13321 aataagtaat ttttcttcaa aaattttagt ttttttgtta gtccctttat atacaagtgt
    13381 attatccaca acagaatatg gcacatatga tttgacaatt tcaacagcca cattattgtt
    13441 tccaatttta accttaaaca ttgtagatgc tgtgatgcgc tttttaatgg ataaagataa
    13501 aaataaaaaa aatatagcat ctatagcgat taaagttata tttttaagcg tgatagtctt
    13561 tggatccgta atgtatttgt taaatattac aaaattatgg caagatgttt atggattaga
    13621 aaaatatatt tttttgtatt atatatccta tgtatggaat caatttctaa tacaatttgc
    13681 taaagggtta gaaaaagtta aagctatggg catagcaggt attatcagca caattgtaac
    13741 aataattgcc aatattttct tcttgcttat tctgaaatgg ggattaattg gttttttcat
    13801 tgcaaatatt ttatcacaga ttatttcagc tacttatctg tcagtattag tgagaatatg
    13861 ggaatatatc gattttgagg ctaaagatcc tcagctaaag agagaaatgc taaattactc
    13921 gattccatta attgcaactg ttgttggatg gtggattaat agtacagctg ataggtatgt
    13981 ggtttccttt atagtaggtg tctcggcgaa tggcttattg tctgtggcat ataaaatacc
    14041 tcaaataatt aatactttgc agggaatatt tatacaggcc tggcaaattt ctgcaattaa
    14101 agaatatgga gataatgaga ctgcaaaatt ttatggaaat acatttgtta tgattaattt
    14161 gttaatgtgc ataacctgtt caagtcttat catattaact agaccgtttt cacatattct
    14221 ttatgcgaaa gacttttatg aagcgtggaa atatgttcca tttttattaa tagctaatgt
    14281 tttaaattgt gcatcaggtc tgttaggacc tatcttatca gcaaaaaaag attctaaatc
    14341 catgatgtgg tctgcaatca taggggcatt agttaatgtg attatgagtg tagtccttgt
    14401 ttataagata ggggttcaag gtgccacaat agccactgtt atatgttcat atgtaatata
    14461 tgttgttcgt aaacgagctg tgggaaaaga tatttttata aagcattatc atattgttgt
    14521 ggaatcttgg ggattattgt ttgttcaggc aatcatagaa atatatttta aaaattatgt
    14581 aatagaaatt ataattatga ttattttaat tattataaat atcagttata ttaagcagtt
    14641 gtgttataga ggaagaaaca ttatttcaat gattaagagt cgagagcaca aacggtagac
    14701 gatgttattt ttataaaaag atataattta ttaatggtcg ataattatga aaggaaaaat
    14761 attcttaaaa aaaagagtat aggatgtcgt atgaaaatag tcgctatagt accaatgaag
    14821 ttaaataaca aaagattgcc acaaaaaaac acgaaagctt ttaccaatgg aaaaccattg
    14881 tgttattata tactttcaac acttttaaaa gtaaaacaga ttgataaagt gtatgtctat
    14941 tgtagtaacc cagatattaa agaatttata ccagaaggtg taacattttt aaagagatct
    15001 agttccttag atcaggatac aacgagtatt acacaggtat tagccggttt cacaaaagaa
    15061 gtagaggctg atgtatatgt aatgacacac acaacagcac catttattag cgaaaaatct
    15121 attgaaaaag gactagatgc cgtgctgaca ggagaatatg attctgcatt tgctgcaaaa
    15181 aaattgcaag atttcttatg gaaagacggt gaaccattta attatgaatt ggacaatatt
    15241 cctagaacac aggatttgcc gcctttatac gaagaaacta gtggatttta tatttataat
    15301 cgcaatgtga tgaccgagtt gcatagaaga attgggaaaa aaccttgcat agttgaagta
    15361 ggtgagattg agtcagttga tattgatgaa gccgaggatt ttatgattgc tgatgcgatt
    15421 tataatcatc ttattttaca aggtagttaa gacaggggga aggttaggat gaacaatgta
    15481 cagattttag attgcacact tagagatggg ggctattgta acaattggga atttggattt
    15541 gataatacaa aaaaaattat ttctggactt atagaatcag gaattgagat tatagaatgt
    15601 gggtttctca caaataaaac agtatacaat aaaaatgtta caaaatatac gaccttagaa
    15661 gaattagcac agataattcc tgaaaaaaga gaaggaaaat tatttgtagc aatgatgaac
    15721 tatggcgaat ataatataga ggatcttcca gattatgatg gttcatctat tgatggaatt
    15781 aggattgcgt ttcataaaaa aaattgcaat gaagcgttga aattatgtcg tgagataaag
    15841 aaaaaaggct acttagtttt tgttcaagct atggtatctc tcaattatac agatgaagag
    15901 tttttagcag taataaaaaa tgtgaatcag tttaaaccat acgcttttta tattgttgat
    15961 agttttggaa tgatgaaagg aaaagattta actcgtcttt tttacatgat tgagcataat
    16021 ctagaaaaag atatacagat aggttttcac tcacataata acatgcagct tgcatattct
    16081 aatgcacaaa aacttataac cgtacaaaca aatcgaagcc tgattattga ttcttcggtg
    16141 tacggaatgg gaagaggagc aggtaatctt aacacagaac tatttgtaga atatttgaat
    16201 gataatgcag gaaaaaaata tcaattaaaa ccattattat atataattga tgaaatacta
    16261 aatagtttct atcagaagag ttattgggga tattcattac caaactatat ttcagcagtt
    16321 cataatgctc atcctaatta tgcaagctat ctgtcatata agaatacttt aacaattgaa
    16381 gcaatgaatg aaatattcga aatgatggat gaagaaaaaa aggtatctta tgataaacaa
    16441 tatatagaag atttatacta caaatatatg gaaactggga aaatacagga ggaatatatg
    16501 gaagaactga gaaaatttct ctcagacaaa aaaattctac tgattgcacc aggaaaaagt
    16561 tctgtagatg aggtagaaag aataaaagag tttgttacca aagaagaagt cgtaataatt
    16621 agtgtaaatt ttgaatattc tcatatagat atagattacg tatttttaag taatttaaga
    16681 cgttttcatg aattaccgaa agaaagcaga actaaatgta ttgttacatc aaatatacaa
    16741 gcatcggatg tatatttacg gacgagttat aaaaagttgt tatctgaaag ggaagttgta
    16801 agagacaatg caggattaat ggcaattaga tttttagccg atatgaatgt ggatgaaata
    16861 ttcttagctg gatttgatgg atattcacat aatgaagatg agaattatgg tgagcaatcc
    16921 atggaaatta taacaaaatt tgcattatta gatgcaatga acgtgcaaat gagagatgaa
    16981 ttgtcagaat tagctaaaaa agtaaaggtg acatttttaa cggatccacg ttgggttcga
    17041 attgataagt agtagtaata atgtttttaa aattttgata aagagatagt gagagaataa
    17101 aaatgggtaa agtcttaaat aaaaaagaag aaaaatattt aagggacgat ggggcgagat
    17161 gaaactctaa ttttgaactt ttaagaatca tttctatatt gtgtattgtt gcttaccatg
    17221 ctaatttgcg tttaaattat aatgcagacg gaattatagt acatggttca ttaactgcta
    17281 accagatttt cttgaattat attggtattt ggggtcagct aggagttaat tgttttatat
    17341 ttatttcggc atatttttta agtgagaaaa gaaacattca ctttgaaaat gttttgggaa
    17401 ttgtattaga atgtattttt tatagtgtat tagtttatgg aattctttgt gcatgtggaa
    17461 tagaaacatt ttcattgaac agctttgtaa aagaaatatt atcatttgct ctaggtagat
    17521 attggtttat tacatcctat ttagcattgt atattctaag tccgtattta aatatcatgc
    17581 ttaacaatct ggaaaaactt cagttgcaaa aaataagtat gttattgttt ataggtgtat
    17641 gctgttatcg tatgttattt aaaacatctt tgtttgggga ttgtgctgtt ttaattgcac
    17701 tatatgtcat atgttataca ctgaaaaaaa cgataataat atttttgaga gacacagaat
    17761 cataggaaca attgtatgta taacagttat accacttctc atatccatag gaatactttt
    17821 caaatttata ccaaatgcaa tggttaacca gatggttcat attggttctc cattgatggt
    17881 tatagcaagt atatttttat tttatgtatt taaaaatttt tcattttaca gtaagtatat
    17941 aaataaactt gcgagaacca gtttaggggt atatatttta catgaaaatc attatttcat
    18001 tgatggtatg aagtctagag gcttaatatg ggatgggata tttggattag gcaaaaggtt
    18061 tttagatagg gatttttgga tttggtttat aattgcagtt gtagttgtgt gcataatttg
    18121 catggctatc gatgttgtaa gagaaaaatt tgaaagacag atttttaaaa ttaagagtgt
    18181 aaagaacctg tgtaaactta tgaatcatgt tataaatata tacccataat taataaatga
    18241 ggagaaaaga agtgaaagtt atatcattcg gagaattatt attgagactt gcagcacccg
    18301 gttattcaaa gttatttcaa agagatagtt tggagactac attctgcgga ggggaagcta
    18361 atgtagcggt atccttagca atatttggat atcaatcaga atttataaca aagcttccgg
    18421 attcagatgt tggatatgcg gcagctaatg ctttgcgtta ttttggagta aatgttgata
    18481 atattatatt tggagaggga cgcatgggac tgtattattt agaaaaagga gcagctcaga
    18541 gaccatctaa agtaatttat gacagaaaat tttctgcaat ttcgttagct gatagtgatg
    18601 aatttaagtg gaaggagatc ctaaacgatg caaattggtt tcattggaca gggataaatc
    18661 cagcattgtc tgagaatctc tttgagacgt ccttagatgc atgtcaggct gccaaaaaaa
    18721 gaggtataac aatttcttgt gatttaaatt atcgatccaa gctttggaca tcagaggaag
    18781 caaaagaaaa gatggaacag atactaccat tggttgatat ttgtatttgt aatgaagaag
    18841 atgcagaaaa agtgctagga attaaagctg agcatacaaa tattaatagt ggagaattaa
    18901 ataaagatgg ctatgtgcag gtggctaggg agattcacaa gaagtttgga tgtaggtatg
    18961 tagctacaac tctgcgtaga agttattcgg caagtagaaa tggttggagt gcaatgttat
    19021 acgaagcaga aaaagataaa gcgtattttt ccacagaata caacatacaa attgttgatc
    19081 gcgttggtgg tggagacagc ttttctgctg gtataatata tgcgttatgt gaaaatatgt
    19141 ccggacaaaa ggctattgaa tttgcgactg catctagttg tttaaaacat actatagaag
    19201 gtgattttaa tcgaactaca attgaggatg tatataattt aatgaatagc ggtggaaatg
    19261 gtcgcgtgtt gcgttagtca acatataaag ataaatcaca gtaattttta tggtgattat
    19321 ctgtcttagt agtgattaga ataacgtgaa aataaattgt tgagcgttta tactgttttg
    19381 aaaaataaat ttaacatatt ga
//
LOCUS       DACECJ010000055        19329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.055, whole genome shotgun sequence.
ACCESSION   DACECJ010000055 DACECJ010000000
VERSION     DACECJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.055"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWO09_001913"
     CDS             99..293
                     /locus_tag="IWO09_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264501.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="IWO09_001914"
     CDS             562..861
                     /locus_tag="IWO09_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264502.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="IWO09_001915"
     CDS             complement(958..1392)
                     /locus_tag="IWO09_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0264503.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="IWO09_001916"
     CDS             complement(1624..2334)
                     /locus_tag="IWO09_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0264504.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="IWO09_001917"
     CDS             complement(2324..3184)
                     /locus_tag="IWO09_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0264505.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="IWO09_001918"
     CDS             complement(3189..3827)
                     /locus_tag="IWO09_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0264506.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="IWO09_001919"
     CDS             complement(3842..4141)
                     /locus_tag="IWO09_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0264507.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="IWO09_001920"
     CDS             complement(4184..5668)
                     /locus_tag="IWO09_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0264508.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="IWO09_001921"
     CDS             complement(5688..6149)
                     /locus_tag="IWO09_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0264509.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWO09_001922"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWO09_001922"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0264510.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="IWO09_001923"
     CDS             7452..8222
                     /locus_tag="IWO09_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0264511.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="IWO09_001924"
     CDS             complement(8495..9322)
                     /locus_tag="IWO09_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264512.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="IWO09_001925"
     CDS             9890..11125
                     /locus_tag="IWO09_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0264513.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="IWO09_001926"
     CDS             complement(12124..12384)
                     /locus_tag="IWO09_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0264514.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="IWO09_001927"
     CDS             complement(12411..13712)
                     /locus_tag="IWO09_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0264515.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="IWO09_001928"
     CDS             complement(14004..14462)
                     /locus_tag="IWO09_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0264516.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15800)
                     /locus_tag="IWO09_001929"
     CDS             complement(14475..15800)
                     /locus_tag="IWO09_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0264517.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17229)
                     /locus_tag="IWO09_001930"
     CDS             complement(15817..17229)
                     /locus_tag="IWO09_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0264518.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="IWO09_001931"
     CDS             complement(17246..18010)
                     /locus_tag="IWO09_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0264519.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="IWO09_001932"
     CDS             complement(18114..18989)
                     /locus_tag="IWO09_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0264520.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="IWO09_001933"
     tRNA            complement(19244..19328)
                     /locus_tag="IWO09_001933"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
BASE COUNT         6140 a         3983 c         2979 g         6227 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctaatac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggca
//
LOCUS       DACECJ010000056        18740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.056, whole genome shotgun sequence.
ACCESSION   DACECJ010000056 DACECJ010000000
VERSION     DACECJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.056"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            256..531
                     /locus_tag="IWO09_001934"
     CDS             256..531
                     /locus_tag="IWO09_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264521.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="IWO09_001935"
     CDS             544..2616
                     /locus_tag="IWO09_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0264522.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="IWO09_001936"
     CDS             2781..4667
                     /locus_tag="IWO09_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0264523.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IWO09_001937"
     CDS             4679..5626
                     /locus_tag="IWO09_001937"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0264524.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IWO09_001938"
     CDS             5646..6173
                     /locus_tag="IWO09_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0264525.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IWO09_001939"
     CDS             complement(6236..6889)
                     /locus_tag="IWO09_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0264526.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IWO09_001940"
     CDS             7021..7863
                     /locus_tag="IWO09_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0264527.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IWO09_001941"
     CDS             8021..8917
                     /locus_tag="IWO09_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0264528.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IWO09_001942"
     CDS             8920..9633
                     /locus_tag="IWO09_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0264529.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IWO09_001943"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IWO09_001943"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0264530.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IWO09_001944"
     CDS             10168..10395
                     /locus_tag="IWO09_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0264531.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IWO09_001945"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IWO09_001945"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0264532.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IWO09_001946"
     CDS             complement(11181..11723)
                     /locus_tag="IWO09_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0264533.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IWO09_001947"
     CDS             complement(11960..12358)
                     /locus_tag="IWO09_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0264534.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IWO09_001948"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IWO09_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0264535.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IWO09_001949"
     CDS             13471..14238
                     /locus_tag="IWO09_001949"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0264536.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IWO09_001950"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IWO09_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0264537.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IWO09_001951"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IWO09_001951"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0264538.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IWO09_001952"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IWO09_001952"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0264539.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DACECJ010000057        18404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.057, whole genome shotgun sequence.
ACCESSION   DACECJ010000057 DACECJ010000000
VERSION     DACECJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.057"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            83..1081
                     /locus_tag="IWO09_001953"
     CDS             83..1081
                     /locus_tag="IWO09_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0264540.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="IWO09_001954"
     CDS             1105..1389
                     /locus_tag="IWO09_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264541.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="IWO09_001955"
     CDS             1470..1868
                     /locus_tag="IWO09_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0264542.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="IWO09_001956"
     CDS             1878..4421
                     /locus_tag="IWO09_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAQ0264543.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="IWO09_001957"
     CDS             4443..6785
                     /locus_tag="IWO09_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264544.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="IWO09_001958"
     CDS             6801..8975
                     /locus_tag="IWO09_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAQ0264545.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="IWO09_001959"
     CDS             8965..9387
                     /locus_tag="IWO09_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0264546.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10268..11179
                     /locus_tag="IWO09_001960"
     CDS             10268..11179
                     /locus_tag="IWO09_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264547.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11666..11932
                     /locus_tag="IWO09_001961"
     CDS             11666..11932
                     /locus_tag="IWO09_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264548.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12004..12345
                     /locus_tag="IWO09_001962"
     CDS             12004..12345
                     /locus_tag="IWO09_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264549.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12400..12819
                     /gene="ssb"
                     /locus_tag="IWO09_001963"
     CDS             12400..12819
                     /gene="ssb"
                     /locus_tag="IWO09_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0264550.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12896..13192
                     /locus_tag="IWO09_001964"
     CDS             12896..13192
                     /locus_tag="IWO09_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264551.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13207..13638
                     /locus_tag="IWO09_001965"
     CDS             13207..13638
                     /locus_tag="IWO09_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264552.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13820..14125
                     /locus_tag="IWO09_001966"
     CDS             13820..14125
                     /locus_tag="IWO09_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264553.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            14529..15017
                     /locus_tag="IWO09_001967"
     CDS             14529..15017
                     /locus_tag="IWO09_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0264554.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            16351..16653
                     /locus_tag="IWO09_001968"
     CDS             16351..16653
                     /locus_tag="IWO09_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0264555.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            16646..17674
                     /locus_tag="IWO09_001969"
     CDS             16646..17674
                     /locus_tag="IWO09_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0264556.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            17730..17948
                     /locus_tag="IWO09_001970"
     CDS             17730..17948
                     /locus_tag="IWO09_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264557.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            18019..>18404
                     /locus_tag="IWO09_001971"
     CDS             18019..>18404
                     /locus_tag="IWO09_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264558.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYN"
BASE COUNT         6640 a         2643 c         3722 g         5399 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagtt ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacacaaact tacaagagag gggagtaaag cagttgcaga
    10201 ggaatcagga agatttgttc gaacagaaac ttgcaagaaa gcattattaa attgaaggtg
    10261 atgaaatatg aagtatttat tgcatggcta ttttattgat tattctactc gcaatagtag
    10321 tactttagcg attctatgtt tgcttcatga attgagagtg gttactagaa aacagctata
    10381 caaactattg aattttgaca gatcaattag taaacaagga gtaacgaatg ctttagtgag
    10441 attatatgaa aaacagttaa tcgatcgaaa aaaatatgga acagaaatgt attattattt
    10501 gacgaaagag ggacatcaaa gcataggtgg gtattatacg ttgccgaaag ttcctgaata
    10561 taatttgaat caccatttac aaattaacaa ttatttgatt aaaggaatgg cattggcaaa
    10621 agaccaccca catttaaaac ggattatttc agaaagacgt accgtctatg aaacgaagga
    10681 tttaaatcct aagacaaaag gaagaaaatt ttttgtgcca gattttattt ttcgttttcg
    10741 agacaaagaa aatcatgaga tcaattggtc ctttgaaatt gagctaacgt tgaaaacacg
    10801 tcgtcgctac cgtgaagcga tttttccgaa atatattcgt gagttgcgaa cacgtcctga
    10861 atcacattta atttatgtga cacctagtgg cgcaattcaa gaagaacttc atgcgtttaa
    10921 gcgtattttt ttcaagcgcg aaactgaaaa agatgcgagt caacaagaat tgtttgagcg
    10981 gttgcatatt ttttcagctt tagagtttga agagggatta aaagcattat tagagaatga
    11041 tccagcagtc aattggcgga cagtagaacg agaaaaaatt tatccgaatg gcgctgttca
    11101 aagtcgtttg aatttatttg aagaatcaac tgaaaatgag gaaaaaccaa aagtgaagtt
    11161 tacgcctgaa gtattgtaaa agagggtgtt cgcttcgctc tttttttgtg ccagtttgct
    11221 ggcaccgttc agacgcaagc gtctgaaccg gctcgttttt aggaaggact aacttgtttg
    11281 atttatccgt tgtcaaagaa gtggacgttc gagagtccct aatcgtatag cagtcattcg
    11341 gcgagtataa cctaaaacat aacctgccaa ttgccaaggg taaaatgcac gctgcgcgcc
    11401 cttggcaatc gtcagaacat gttttcgagc cggttatgcc gaacgaccgc tatacgaaaa
    11461 gggcctctaa acagaagtcc actttgatcc ttgacaactg aaacccaaaa tcaaacaagc
    11521 aggccttcgt ctaaaaacga gccgaatgaa gaccttgggg gttgcacccc caaacccccg
    11581 tgcccccgac acactaccga aaaggtgggg gtgggtggcg aaaaaatcaa ataaaacatt
    11641 ctattcaaga aaggaagaaa caaccatgac aactaaaaac gagcaactga aagaacaggc
    11701 aagaaaagag ttgcaagaaa gaggtttgat tattgagggg agctttgaag gagattttga
    11761 aacctatatt ggttgttatg caagaccgat aaacaaacct acagccttag acccaatgaa
    11821 cgaacaagaa gtaaaagaac aagaaaaaca tgcagtgaat ggttttccgc aagatttcac
    11881 tgaatggtat gagtgggaaa ttaacaacgg gaagcttgaa aacttcttat aaaagtcaga
    11941 tgacgtgcaa agagaatgat tctctttgta cggtaaaaat ataaagaaag aaggaaaaag
    12001 aaaatggcaa aaaacacacc ttatcaagta ggagacattt tttatagtag ttggggctac
    12061 gaacaaacga acgtgacttt ttggcaaatt gtgaagttaa cagaaaaaac agcatggttt
    12121 cagcctattc aaaagaaaac ggtcgagcat tattcgtcca tgagtggaaa agttgttcca
    12181 atgaaagaca agtttataga gtatccttta gcaaaaacta aagacagtat tgtaaaaaaa
    12241 cgaattgatc cagaaaccta tcctgatatg ttatgtggtt atcgtagttg ggacacgttt
    12301 catagatata aaaatcaagc tgtttatgaa agtagttatt attaagggga tagtttcccc
    12361 tttcaaaaat aataaataga agaatggaga gaaaaaatca tgatgaattc agtgaattta
    12421 gtaggacggt taacaaaaga agtggattta aaatacacac caaaaggcaa tgcgacaggc
    12481 acatttattt tagctgttaa tcgaaattat acgaatgcaa acggcgagcg tgaagcagat
    12541 tatatccgct gtgtcatttg gcggaaagct gcggaaactt tagcaaaata tactggaaaa
    12601 ggaagtttga ttggcattaa tggacgtctt caaacgagga gctatcagaa tcagcaagga
    12661 cagaccatgt atgttacgga agttctagtg acagatttct atttgttaga gagtaaagaa
    12721 atcaatgagc aacggtcaaa atcaatggcg aataatcaaa ataattcttc aattccagga
    12781 aatgagattc aaatttcaga tagttatttg cccttttaag tgaaaaataa ttttgttcta
    12841 tacctgattt cttcaggtat agaacaaaaa ataaatagag atgaggcgat tttttatgaa
    12901 aatcattgac agttacgatc aacgaaccat gcaatatttg ggctcgtttg attatacaga
    12961 tgaaaatatt attaattatg ttgcaagttt acctccttat caaagcgttc gcttggtaga
    13021 ttttgcaagt gatgaattgg tattaacaac gatgggcaac tttttagatt atgtcggaga
    13081 caaaaaatgg ttggaaaaaa ttttgtctaa attgattgcg aaacaaacag gagaagaacc
    13141 cataaatgcg attcaattgt tcaatcgata tgaacaagaa aacaatgatt aaagaggaga
    13201 gaaaaaatga aaagattaaa acgaagtatt tttattgtgc tagcattgat tgattttgtt
    13261 gggacaggtt gtgaagcaat tgacgttcaa aaagctccct tatcggaaat aaaacaagca
    13321 ataacaaaac aagatgaaga acaaccgcta aatacacaag tagaaatagt gaatcaaaat
    13381 aaaccgagtt tttctgaaga agatttttcg ttagaaaatg gaaccaggca atttttttca
    13441 gatttggatc gtttaaatcg ggtaggtgta gcaagtgcac ttttgcataa aagcaggatg
    13501 ccaacagaag aacgagaccg attatacatt aagccaactg gctggaaaca aaagaaaatg
    13561 aagaatggcg attatcttta taatcgttgt catttgattg gctatcaacg tgcgacaagt
    13621 ttccccataa gtatgtaata cgaaacggga ggattaatct taagattaat cacaactaac
    13681 ctatttgaca gttggaatga aagctatcat gtctgctgaa tcaactggtc tgatactcct
    13741 acatgcaata aagtctacta ggaagttatt gtatgaagct aggtaaaggc ggcagaacaa
    13801 tttctaagtg tggaatcata tgaagttgga tatgccggga cgctataaat tactcagggt
    13861 gagactgttt ttaattaaac tgacgaactt gcgaatgtac gggtctataa ccgacttata
    13921 ttcgaaagat ataagattgc gaatgcaaag aacattatcg aaaagtaaga atgttggtca
    13981 tgaaattcga gatggaagac tagcggtgta tctgtcaaga gttggtaaaa catgtttgat
    14041 tgattcagat gagagtggga atgcgacaaa acgttttaca gaagaaatag agcaactagc
    14101 agaactgaat atacgatatt tttaaatact ttagcggaga ataaaaaaat cctctacaga
    14161 aaactacgga gggcaacatg tgctattgtt tcaacaatac atttttaatt tttaccataa
    14221 caaaaaagta cttgaaaaaa aacagaaata tgttaaatta aaaatgaaaa aatggaagcg
    14281 gttacttatt cttttgaatc taagataaaa attcaaaaga ataacataac ccaaatatga
    14341 ttatacttga gattctaaat atttatcgaa aaggaatttg ttttaaggaa aaatgattct
    14401 atggacggtt attgatttat atttaccaaa tggctaataa aatagtattt cttatattgt
    14461 atagtttttg tttttatgtg aaaacatgag caatagaatt gtactagtag gataaggatg
    14521 gatggaaaat gaaaaaaact atttttgttt tagcgatttt tatatgtttt gggctacttt
    14581 tggtaggcta cttgttgttt aattctaatg aaatgagaac tatttctacg aatgacttgt
    14641 taaaaaaaag tgaattaaag gataaaaaaa aagaatctct tgcctttata atgtttactc
    14701 aagaagagtg tgagtattgt atagcaataa atccaatcat agatagtgta aataaggaaa
    14761 ctttgaataa gataaagaaa tatgatatat ctgtagattt tcaatctaaa caaaagtgta
    14821 aaattaagtt tgttccgact attgttgcat atagtaagaa taaagaagtg gggagaattg
    14881 acaatgaatt agataatttg tatcgaaaaa agcaagatgg ttcaattgta atagacaaga
    14941 aaaaaacaaa gacattgctt atagattgga tgaaaaaaat ggataaacaa ggagaatatt
    15001 tgtatggaga agattaaaca gacatttccg ttaattaata aatattggaa taagaaaaaa
    15061 atttttatat tttttattac gtttctgctg tcatctttat tttctctaat tttcccgtat
    15121 ctatttagta atgttattga tttatattta gagaatgagt tatcacagaa tattgtgcga
    15181 atagtaacga ttcttatatt tcagttattt ttagtcttaa ttaaagaagt gtatggatat
    15241 ataaaaacta tttatgcatt tgaagttgat ttatcaatga aaattgattt tcttaaggtt
    15301 ttacaaaaaa aaaatctttt tttttcgcaa acgaagcatt caggagaaat tcaatttagg
    15361 atgttttcgg atatttctac tatcgtaaat actatcttta ttttttctgt tgaattacct
    15421 gtgataggcg ttttagttct tgtagcggta tttattataa gtaaagtgaa tcttattttt
    15481 ctgatttttt cactaataat gatgggagct atggtgttac agtataattt atttcaatta
    15541 ccgcttaaga aaacaatcga gaatacaaaa agtgttaatc aaagtattac aggagaattg
    15601 aatgcacatt ttgatcaatt agaaattata agcgcatata attttgagaa tatctctatt
    15661 ttaaaattcg attctagatt taaagaatta tttaatgttt tcaaaaaaca aaataaatta
    15721 ctaacactgg gaagctgttt ggcacaggtt attttccaat tatggattgg aggaatattt
    15781 cttattgatg ttattttatt agctaaagga acaataagta ttactaattt tgtaatagta
    15841 tttatgtacg ctaacatggt ccctacttta tggactactt taattagtat tatttggaaa
    15901 tggcaagact attcagtgtc attaaataga tattacgaat attattcaaa taacaacaaa
    15961 gtggctggca caaaaaaaat aaataaaata aattctatta ctggtcaatc attaaatttt
    16021 tcatataaca aaagaaaggt attaagcaat ataagttttt caactgaaat gaataaacta
    16081 ttcatgatat caggagaaaa tggaacaggg aaaacaacac tgatgaaatt atttactaaa
    16141 cagttgccgg ttagtaataa tcaattattc gtcaacggga tagatattaa tatgattgat
    16201 aacgaatcat taagaaggtt aatttcaatc gcaacacaaa taccatatat aattaatagc
    16261 tccgtgaagg agaatatttt aatggggact aaaaactatg ataaaaaaaa attcagtaaa
    16321 ttaatagaac tattgggaat aagtaaattt atgacgaaat taggtattac tttggacgac
    16381 aatatttatg atgaaaaaat attattatct ggaggagaaa aacaaaaaat aagtttagct
    16441 agggcgctta ttaaatcagc ttcagttatt atattagatg aaccatttag aaatttagat
    16501 aatgaaactc gcgataattt gagggattat atactaaata caaaaaaaga agtttgttat
    16561 ataattataa ctcatgatca cattctagat aattttttgg attcaaatga tgaaatatta
    16621 tatttaaata aggagtatga taataatgaa tagcaaaaaa cttcagatgg gtgaaattgt
    16681 tagaggtcct agtcaaatat ctattgatgt tacatataaa tgtaatttta gatgtaaaca
    16741 ttgttacaat gcgagtggtg gagacaattg ttttatggat aatgagttga ctgatgatga
    16801 actgttagaa cttacaaaag atatatgtaa tttaaagcca tataatattt gtttctgtgg
    16861 aggagaaact ttactgagaa aaaaaatagt tttatcttgt gccactttat taaaagaggc
    16921 aggtattcct aatattgcta ttgttactaa tggatcatta gtaactcagg atgttgcaaa
    16981 agaattattc aaagcaggta ttaatcgatt tcaagtaagt ttagatggaa aaaatgctag
    17041 tagccatgaa gcacttagat tatatccaaa cagttttgaa aaggcaatta atgctatcaa
    17101 attatttaga gaattgtccg aagaagtgga tgttgctttt tgtcctacta gttttaatta
    17161 caatcaatta cgagaagtat atcaactagt aaatactctt aaagtgaagt ctttaagaat
    17221 tcaaccattt atgcttagtg gtaggggaat gcaaaatagg gaaatagtgc caactgtttc
    17281 tcaatataga aaactagttt ctgatattaa ctatattaaa agaaatgaag attataccaa
    17341 gatagattgg ggagatccaa ttgatcatat catccgacac agaactttat tgaaagattt
    17401 ttcgaatttt gtttctatta ggtcagacgg aggattaatg gcatcacctt acttgccaat
    17461 tcaagttgga aatattaaat gtcacagtct ttctaaatat tggcgagcag gactttataa
    17521 agttttttct ttagatttag ttcaagatat agctactcaa gtttcttcaa tacaagatat
    17581 gagtaaagaa ttaaaaaaag gacatattaa atggcttaat gaagatatag aaattgatat
    17641 tctcgatgaa tacaaacatg atattttaat ttaggtttat cattggaaag tttagataaa
    17701 caaagtactt ttagaaagga gggaaaaaaa tgatttctga acctcaaatg aataatatgt
    17761 atgaaagccc aactataatg ggagctggtg aagctgcgcc aagagcttgt tcagcaatta
    17821 tggtgtgtgt gttattagca tctgcaggag taggtatcca agcaggagcg gtgtatactt
    17881 atgctgtagt tgctacagta gctgtagctg cagccgcagt ttggaaaaca gtaggattag
    17941 ttggttagga attaatagga gactaactgt ctcctattga ttccaatgat taagctaagg
    18001 aggaatttta agaaaataat gaaatacaat aatttaaaac cgaaattact taatgagaac
    18061 atagaatata taagagaaga aaattatgga tatttaacca tagttcccaa aaatggaact
    18121 gaattgaaag ataacgtttt caatccaaca gtaagatata tattggctaa ctgtactgga
    18181 aaaaacagtg taaaaaaaat agcgaatggt ttaaaaaaga agtttcctag tttagaatat
    18241 gaaaaattct atcaagatgt tgtaggaact attaaatttt tcaaacacta taatattatt
    18301 gatataggag attatagtat ggaagaaaag aaaatatata aaattgaaca aaatttagaa
    18361 ttagaattac taaatgaatc acattatgaa gaaatttata attt
//
LOCUS       DACECJ010000058        17723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.058, whole genome shotgun sequence.
ACCESSION   DACECJ010000058 DACECJ010000000
VERSION     DACECJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.058"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(146..2518)
                     /locus_tag="IWO09_001972"
     CDS             complement(146..2518)
                     /locus_tag="IWO09_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264559.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            complement(2550..2744)
                     /locus_tag="IWO09_001973"
     CDS             complement(2550..2744)
                     /locus_tag="IWO09_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264560.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            complement(2891..3226)
                     /locus_tag="IWO09_001974"
     CDS             complement(2891..3226)
                     /locus_tag="IWO09_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264561.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            complement(3238..4830)
                     /locus_tag="IWO09_001975"
     CDS             complement(3238..4830)
                     /locus_tag="IWO09_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264562.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(4849..5271)
                     /locus_tag="IWO09_001976"
     CDS             complement(4849..5271)
                     /locus_tag="IWO09_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264563.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            complement(5313..5588)
                     /locus_tag="IWO09_001977"
     CDS             complement(5313..5588)
                     /locus_tag="IWO09_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264564.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            complement(6134..6364)
                     /locus_tag="IWO09_001978"
     CDS             complement(6134..6364)
                     /locus_tag="IWO09_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264565.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            complement(6386..7192)
                     /locus_tag="IWO09_001979"
     CDS             complement(6386..7192)
                     /locus_tag="IWO09_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264566.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            complement(7203..7847)
                     /locus_tag="IWO09_001980"
     CDS             complement(7203..7847)
                     /locus_tag="IWO09_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264567.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            complement(7863..9068)
                     /locus_tag="IWO09_001981"
     CDS             complement(7863..9068)
                     /locus_tag="IWO09_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0264568.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            complement(9068..9445)
                     /locus_tag="IWO09_001982"
                     /pseudo
     CDS             complement(9068..9445)
                     /locus_tag="IWO09_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            complement(9576..11453)
                     /gene="ltrA"
                     /locus_tag="IWO09_001983"
     CDS             complement(9576..11453)
                     /gene="ltrA"
                     /locus_tag="IWO09_001983"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0264569.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            complement(12141..13835)
                     /locus_tag="IWO09_001984"
     CDS             complement(12141..13835)
                     /locus_tag="IWO09_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ0264570.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            complement(13998..14591)
                     /locus_tag="IWO09_001985"
     CDS             complement(13998..14591)
                     /locus_tag="IWO09_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264571.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(14592..14924)
                     /locus_tag="IWO09_001986"
     CDS             complement(14592..14924)
                     /locus_tag="IWO09_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264572.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(14974..17505)
                     /locus_tag="IWO09_001987"
     CDS             complement(14974..17505)
                     /locus_tag="IWO09_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264573.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            complement(17502..>17723)
                     /locus_tag="IWO09_001988"
     CDS             complement(17502..>17723)
                     /locus_tag="IWO09_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0264574.1"
                     /translation="LHDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQE
                     YVEKLIKNEEISEMIGKRLLLESEKVVKE"
BASE COUNT         4523 a         3709 c         2784 g         6707 t
ORIGIN      
        1 ccaccgctaa accattgttt cgtgcaaaat ccacaatatc catttgcttg catcgatcaa
       61 tgagactcat ttttttctct gtcattttca aacctcccaa aagaaaaaag actatccatt
      121 tttcagatag tctttcttgc ttgttttata cgcgttcttt ttgtgacaat tgttgttcat
      181 aatagatttc attttttcgt tttgccactt ctgacactgt ctcaagtgaa aatcggaatg
      241 gatttcctag cgcaaattct tctgaaggta acgccgtttg gatctctttt ccataacttt
      301 ttccttttaa aatcacttga ttatgctgcg gatcttcaat aaacgccgca tccttcaaat
      361 gtaacgttcg gcccgtgtct tcttccagga ctttcccgtc tttcttgtcg atccaaagtg
      421 agccaacggg cacaatattc ttttgttctg ccgtttctac agtattttta tgctcctgct
      481 tcacttctgc tggctggatc tctggttctc tttctctgta ttgattgatt tcattagaaa
      541 tggtttgaat gaattcattt gcgacacgac tgacatcatt catgattttc ccacgttccg
      601 cagcttcctt atcatccaac gtctgactat tcttcgtcca tgtcgcaata taaggaatag
      661 ctttctccga ggtgtccatc ccaaaatgct tgcacacaat ataggaggtc atttctgcct
      721 gtagttcttt tgttgcagta tctaattctg aaaatttgtg cattctagca tgggctaatt
      781 cgtgaatact tgtggctaaa tgctgcgtag gagtatttcg agtattcatg acgatttctt
      841 cttcgccagt aaattcgtta cgaacgtacg ccccacgtgc ttgtcctaat tctctttgat
      901 agacttcact ttctgtcata tctcgaattt gaattccaat atttttggct accgcttgaa
      961 tcccaacttg aagttctgtc tgccctgttt gatccaattg aaaatcaaag acgcggttcg
     1021 ggaagatttt cggatagtct tcaggtttca attgtgtttg tgtgatatca aacacttttt
     1081 caatcgtgta gtaaggtttt ttgatcactt cgatttgacc actttcgatt cgttttttat
     1141 cttctggtgt agcctctgtt tctcgaacaa agccacgtcc tttctcataa aatcctttga
     1201 ccgtttttct gacaaagatc ttgttccctt gttctccttt tttgatatag attccttctt
     1261 ttttcaacgc ggcacgacct aaacaagccg ttgcgcctgg aaattgttcc tcgatcaata
     1321 attgatttct aaaggatcgt tctggaaaac gagacataaa attcaaaagt tctttgacgt
     1381 cttcaggtga atgagtatgc tttttcaccg catcaagcgc ctgttgggaa agtgatttga
     1441 cttcttgctt aatctcttct gccgttcgtt tttgataacc cttttgtttt gtttttgtct
     1501 ctttaatctc gttttgtact tttgaaatag gttcattttt gactgtttgt tcttgttcct
     1561 tttcaggtga ttgttcgcac aatctagcta actctaaata gatggggtga ttactattga
     1621 cgccatcccc tagatccatt cgtatctgat cgactacttc tccattcttc acttcttcaa
     1681 aatagaattt ataatagcct gcttctgcgg cgtattggtt atccagtgtt ttgagttgat
     1741 cgatcaattc tagtgttacg atctccccac cataactttt caaccctttt gctggatcat
     1801 tttcgttgaa ttctactcga tagaaatgat cggtttcttt ctccttcaca gaatcagtag
     1861 gtgtagcttc ttcaagtaat tcacgaacag ctgccttttc ttcttccgac gtggcttggt
     1921 attttttaga ttcctcaaat cccccgtttt cattgagttc taaagcgact tttcgatagc
     1981 ctttcagaaa atagatagtt ggattctttt tccattgcgt cactcgtccg ctgtcaatga
     2041 tcgcttgtac ttcaggaaga agattttctt ttcctttctc tgatttttct ttcagctgtt
     2101 ctaatgtttc caattttcgt tggtattttc ggatcgtggc agctgaaaaa gtcgattcac
     2161 ctttttcaaa gcgttcgatt tcttctttga ttcttggaat attttcgatc gtcatgtcca
     2221 ggccaccgta cttgttataa cccatttcct ttagctcttc ttgtctttca agtttttcta
     2281 cctgtttttc ttgttgcttg atctcttctc tcaaactttg tattttattt tctatttttt
     2341 cttgctggtt aaaccatgaa cgaccatttc gtttgtcatt cattggttgc ccatttgtca
     2401 atttttggtg atcaaatagc ttttgaaatt gattatttaa ttcgttttgc aaccgtacta
     2461 attttctttt ggactttccc aaacgactat tatctagtgg ttctttttct tctgccatgt
     2521 ccgtttcctc cttgtaaaat atcgtttgtc taaacaaatg gataattttc tactgcatca
     2581 acgaacatct tcgtagtcag atttaacgtt aaaatattgc cattttgata cgcttttgca
     2641 gctttgtctg tgactacaaa agctctacct gtttgaattt ctgtcacaaa aacgagatta
     2701 tccctgcttt tttcaaccac aacaccaatc gttgtctgag tcatttcttc ttcctccctt
     2761 cactttggaa tctcactttc ttttgatata ataaaattga atcgcaccac tcagtgaatt
     2821 ggcggaaaac aattcattga aaaagagtcc tggacaatca cgttcaggac tcttttacgt
     2881 ctctatttat ttaacattcc gtgagagaaa tagactcttc aatgctttca catgggaaat
     2941 tattgattcc ctcttcattt atttcccaag tagtataata aggcgctaac ttaccttcgt
     3001 attcttgaat aataaaatat ttttcaccct tcacatgatt acaacaatac acaccagtag
     3061 ctgggtactc aacaactaaa aattgatttg gatcaaactg ttcgtttctc aacatttcgc
     3121 ttatctgaaa caaatgctct ttttctaaac aatcaggaag ttgttcgatt cgttcttctt
     3181 ttagtgatcc ttctatgact ttccattgat ctaaatattg tttcatttct cttcctccta
     3241 ttgtctttct tgttccattt ggattcttcg ctgttgctct tcatatgctt tacttgcgtg
     3301 gtatttttct tcatgtgcat taattgttcg atttaattga cgcaagcttc gctgcgtgac
     3361 aactctttgt tggaattgtt tcctacgctt tacgaattct tgataactat acttgtattc
     3421 atttgttggc aactgtttga gtgttcgaaa ggatcgttca ttgtaaatag attgcatctg
     3481 attgacactt tctattaatg gttttatttg actatcctgt ctcttcagtt ctgttaaaat
     3541 cgcatttcct gtgcgctttt ttaaatcaaa gagggcgttt ttttctgcat tctttgtttc
     3601 tcttttgctt tccccataaa ggcttttggc ttcttctttc aatgcttttg aaatctgtat
     3661 atattcttga tatccaggtt cttttgttaa aacatatttg gttaactcat caagttttcc
     3721 ccttgtaaat ggtgaaagct tggttcgatt ttcatctcca taattccatt ccgaacgttt
     3781 ttcaggtagg gttttatata cttcatttaa taagttcatg gcgtgtcgat cgtaacgtgc
     3841 tggttctttt actacttttg ttaaggtgtt acgcaaatca ctttttcgtg tcaataaatt
     3901 tcgtttctcc ataccgatca ttcgatcctt ttcataagag aaacgttcta actcatgtac
     3961 ataattggtt ttcattgcgt ctaatgttgc ttgtcttctc ttccccttcg gtacgatata
     4021 actcacacca ttttcaatct cagtatacgg tttacgcgta ttttcttttt caacaactgc
     4081 aaaatgaata tggatatgct tagtgttacg gtgaatcgtt gccatccagt aacctgtttc
     4141 attcaattgt tctttctcga ataatgtctc catcattttt cttccagctg ctttcaaacc
     4201 ttcttcatct aaggtatctg ttttgggatc gtacaagccc attttttcaa gatacgaatt
     4261 gtcgtgagag ataacatctt gataaatgat tgagcctttt tgttgtcctc tttggaagat
     4321 ttgtttgatc tccttcagat cacttccttt cacttcgtct gaagccatag aaaaaactcc
     4381 aggaagattt cctgaagctt ttttatgtgg ctctgtttga tattttttca ttgcttggtt
     4441 caaccgttgg tattcttttt gttccctttg cgtcagttct ttcttttgtt ctaacgcata
     4501 gtgacgggtc atataagaaa gatatttggt aaaatcttgt tcctctaact tgtcaaaatt
     4561 gattccatgt tccatcaacg attgagcttc attcgacgtt tcactgaatt ctttatccgt
     4621 cttactttcc gaaaaagttt ctccttcagg aatataaaaa tcctctaaag cattcttgat
     4681 tcgttgcagt tcctgttctt cttcgggcgt acgtcgttcc atttcttcca gtgctttttc
     4741 tcttgtcata taatcaatgt atgttgaaaa agatttttta ttgggcaagg taaattgaca
     4801 agtaagaata actgcaggcg atttattcat cacgttctcc ttctactttt atctttttag
     4861 aatcttcgtt ggatcaaatg gatctactct ggtatgaaca gattcattca ctggtttctt
     4921 taacttagaa gaatattttt tggtttgatt ggatttaatt tttccttcca cacgttcttc
     4981 tgcttcttga taaacagttg aatttgtacc aattagtatt cctgtatctt tcaaatgaag
     5041 atccgcaaac tctgaaacca tttcggttaa aatagaaatt tcttttccca ttgcattcaa
     5101 tttaatttga attggattta ttttttcttc gatttttgga tcatacatca tacacaaatg
     5161 tctgactgct tcactataat tagatatatc ttcatgagta gaaattattt catcaagtac
     5221 ttcgatatgc atatcgtaaa gatatacatg ttttcttgtt gcttttgcca aaattgattt
     5281 cctcctttta taaattcaca atactatttt tattattcaa ttaacaaacc aattaattga
     5341 ttatttgttg caatatattc ttctaaagtt ttcgtattat aagtttgaat atccacaaat
     5401 ttttctaaca accgatcaaa ttggtctttt ttataattca gaagagcgtg atcaaaatca
     5461 ttttcgatca aaacttttag gtattgattt cgactccatt ttttgccagt cttgaccgta
     5521 agttcttcac aacgtttgtc aattttatta atatactttt cttcaatatt tctaattaat
     5581 aagtccatat tttcctccaa atttattatg tgtagggata tgtgtagcta cacatatccc
     5641 tacacatttt ttgattttgc tacacacaaa cgcttatttt actgcccaca ttcgctttca
     5701 tcctacccac aaatctacac acaaatccaa aattttgata tcatttatct ttcttagaaa
     5761 cgtggtaggc acagcctacc taacaccttt cgtgagaaag tgatatcacg tttccttatg
     5821 ctctttaaaa attaaggtta tttgtgtatt tagacaataa aaaatccttc taactttgtc
     5881 gattagaagg aaatattttg tcatatgaaa aatgaattta agaaaaagtg tatgctataa
     5941 ttgatacgct gtcttcctgt ttggttgaaa tcttcagcca gaaaggagga gtgttaaatg
     6001 ttttatgtgc ttgatgtttg cgttagaatc gtggttaatg ttttctccca tctcattaac
     6061 aaatggttaa ggtgatatag ctgattcata agcatttttt atatactttt ttcacataaa
     6121 atactcctgt tttttatttg ttatcagctg taaaatattt tttagcagct acatatagag
     6181 cttcaaatga tctgaatttt aaagtatcta aattctttcg accattcttc aactcagata
     6241 ttgttgtgtt agataaattc acttctcgac atatttcttt agcttttaca actttgagat
     6301 aggctttaat caatactttc tgctcagcga aagtatcttt gtcagccaag tttgttcgct
     6361 tcattttttc actccacctg tccttctaaa tcttttcttt tgtcatacga tcaatttttt
     6421 cttgaagttt ttgaatgatc tcttcttgat ctttcagttg cgttttcgat gtttcaattt
     6481 cttgctggta gctattaatc ttgttttcag tattttcctg atcatctgcg acttgataag
     6541 gcaattgatc ttttagcttc ttcactaatt cttcattcaa atcaatggtt gcttggaaag
     6601 attccatttg tttttgagct gctttttgtt ctttcttgta tcctttcact ttgtaattga
     6661 tcgcgtgttg ttcataatct tcatctttca cttgatcttt aaccttatct tcgtgcacgt
     6721 aataaatcag atcttgtggt tcttgcatat ccacttttgg atcaattttt tcaggaataa
     6781 tggttaaacg catggcgata tattcttctg gtacatgcgt tagcttcact gcaaaataat
     6841 catcagacac cttgatcact ttggctcgta ttgcttgata atccccttta gctgtcttta
     6901 cttctacgcg ataattaaga ttagccagtt cttttaacaa gctttgatct tcatcatcga
     6961 tcgcaaattc aaaaactaat tgctggttcg ctcctttttc ttttgaaacc attgtcagct
     7021 ctttcgtgtt taaaaaggcg ttcgtgctta cctcatctgg tatatctacc ttctgattcg
     7081 tataaatcgg actagcaaga atcaaaaagc cgagaatcat aaagattccc agccattgaa
     7141 gataactttt tcgttttttg aaacgttgtc tttctccttt tttcttactc attttcttcc
     7201 tcctaacgag tgaaataact aaagtctatg actttccatt gaccatctac ttgtttttca
     7261 gtgacagtta atccaccttt tattttttgt ggtttgacat ctttgaattg cggattgccg
     7321 attgtataat caacaaatac gacaaaattt tgttcctctt ttacactgct tgtttgagca
     7381 taagtttgca cgtcacctaa tgtaaaggag taacttccgt aatcatcatg ttggtgatca
     7441 tcgtctgcag gacttaattt gtcgatcact tcttgggttg cgacatcttt tagtttttcc
     7501 gttaattcat ttctacttaa tgatttatca atggccaatt gaccgaactc ttctgccgtc
     7561 tttctttgtg cttcagttac ttcctctttt gcttttgtgc catcttgttg cttcagctga
     7621 tcgatttctt ggtttttctc attgatggtt tgatttaact tgtcgatctt cgcctgtgcc
     7681 tcagaggaat tttttttgag cttctcagac gcgtttttag ccacttcttc aattttaaag
     7741 gtatttgtct cttgttcttc tttcagctgc caaaacaacg cgatattgcc gattaaacta
     7801 acgctaattg cagctgttaa gacgattttt aaagttttat tttccatatt ttttctcctg
     7861 atctattttt tgctgctatt caaagatggt aatggatcaa tccatgtacc atcgtctgtg
     7921 aatgcgtgtc cttgtgcttg acgccaatcc attcgagtga aacctaaatg caagtgtgct
     7981 gtatctcgta tcccgattac ttgaccaact ttcacttgat cgccaacctt cacacgagaa
     8041 tttcctgtac tattcgcaaa ttcttgatat accatattca aaccgtcgta attaatcacg
     8101 atcacacatg cacctaaacc tgaaatacca ggattgccaa cgtacacgac ttttcctcca
     8161 tgcaccgcgt gaatttcact tcctggatga tcgactgaac caaagtctaa tccatcatgg
     8221 aagccattcg gacggaattc accgcctgga tttgttccaa ataattgacc accactgaag
     8281 gaacttttat ctaaactgct gccagggaat ggattcgtcc attcaccagt gatcgtgccg
     8341 ttgttattgc caccttcacc ccaatgtttg atggcgaatt catcatattc ggttaattta
     8401 ttagaagtga taatggacat acaacctgta taatacggac tatcttcagt aattcctgca
     8461 gcggcataac cacctcgtcc taagatcaat aagcttgcgc cagggctttt atttcctaca
     8521 catgcattaa aattggattg tgtggctacc ataaagccgt aataggagaa ggctgctttt
     8581 gagttgggaa acttcatgta ccagccacct tcaataccac cgattcctcg cgcagttcct
     8641 ttaggaaaca gacaaccatc aaaccaagta atcccaaaat agttgagatc atttcttgcg
     8701 gacgccgacg taccaaacgc tgattcataa ccgtattgcg aaaggatcac acttggtaat
     8761 aatttatatt tcactgctgc acctacagca tattgcgcaa tctcatcaga aaaagggcct
     8821 cttcctttga tttctttata aatcggtaag ccttccgaat attctcctgt aaaagcttcg
     8881 ccaccactac ttgaatctga atcagaatta tcaatcgaac ccgccgcaag tattagaaaa
     8941 agggtcacga acacagtaat caaaatagta atcaaacctg cagaagtacc aaacaaccac
     9001 aaaaataagc gtcgctttgc acgacgctta acatactgct tgattaaact ttcattttgg
     9061 ttttccatta ttttcctcca tcaaacaatt ttaattgacg tttgttcgga ctaaaatgaa
     9121 cacgaatggt ttcttttgag ccaaaattga tcacagcgtc tccttgattt tgttgaggaa
     9181 tggattctaa ttctgagttt gtcattgatt taccaactaa tccttctata tgacgtaaaa
     9241 tagattcatc catacgcata aagatcttcg tagaagaaaa ttcaaagact ttcttcagtt
     9301 tcgagaagtt atcagaagac atattttctg gcaccatttc ttgtggggat tgtgtagcta
     9361 aaacagtcat tgcttctact ttacgcattt ctcgttgaaa gtttgtgaca taatttaccg
     9421 tttcaataaa atctggatta atcgatactg ataggtaaga gtttgatatt atctccgtct
     9481 tttcccccag tccacaccgt acgtgcgact ttcaccgcat acggcgttcc ctcgtctata
     9541 aaagacattc tgttttcccg tcctacaaat atttattaag atatttcttc gttaccaact
     9601 tttaatctca actcattgag tttttctttt tgttttggat ttaattttag atattttaga
     9661 agttcctcaa tcgtttcact ttgtgtagcg tgtattaccc gatgaacctc ctcagtaaca
     9721 atcactaggt tttcatagct atcacttttg ctttcatgat aagggataat atgatgacaa
     9781 tgcatttcag ataagataag ctcctcacct gttactgcac atcttccctt ttgagcgatg
     9841 aattttgaaa ttcgattgtc cgcaaattca atcgttgctc tcttactaag cactggattg
     9901 ctacacaacc acgatatctt ccattcttct acggtttgtt gcttcttatg aatggcttcc
     9961 ctaccttctt cggtgtattt attaatattt ctttttttac acatcggatg tctgtgttga
    10021 atatacccta ctggtaatat tggaaaccct cttatatatc gtatttgtcc tgaccgtagg
    10081 taaggcttgt atccttcgta ctttccgtat tgtccttctt ttgagattcc ttgttctcgt
    10141 agtcgtattt caaaacttgg tctgagttct cttgcaattt ttcgcattgc tacactacaa
    10201 tgagtcgcaa ttttgaagta attatgaatc ccaataacca tactgttata gactccaata
    10261 tagataaagg tatctttgct attaggactt ttttgaattc gtttgatttg tttttttagt
    10321 ttcactttaa tcatgctttg tgcttttgta cctatatgcg tattaacaac ctttttgtta
    10381 cctttggtca ctaatttcat cgtaaagcct aaaaattcac tttcttcttt tgaaagattt
    10441 gtgatttttg acttttcttc agatattggt aactttagac gctcctgtaa ccacatttta
    10501 gttgcataga aaactttttt cgcttcatta taatttctac agaaaatttt aaaatcatct
    10561 gcgtatctca ctaggtatat ttcttttaga gttgtactct ttctgagctt gtgatagata
    10621 ttacctttat cccaagctcc atttttccta aaatgatgtt ttagctcttt ggcatctttt
    10681 tcctcccact gatttgctat ccaccagtca aattcgttga gattgatgtt cgcaagtaat
    10741 gggctaagta ttccgccttg tggtgttcct tttgttgggt atatcaactt cccatttgga
    10801 agtactacgg gtgctcttaa cattttccta atgattaaga gaagctgttt gtctcgtata
    10861 cccatagacc agatttgtct catgagttta gtatgattga cttcgtcgaa gaaacctttg
    10921 atatctacat ctaccacata ttccatgtga ctttgattca ttcgggccat acaatctgct
    10981 attgcgtgtt ctgctgatct atttgggcgg aaaccatagc tgtgattatt gaattttgct
    11041 tcacatattg gttctaatac ttgtaagatg cattgctgcg cgattctatc ccatattgat
    11101 ggaataccca acggtcttaa tttaccatta tatttaggaa tttctacacg ccttactttc
    11161 cgagggttat aatgtgcgaa tcgcttccta acaatttcta cgaattcatt acggttgaga
    11221 ttttcaatat ctttaatcgt tactccatct tctcctcttg tcacactacc gtgatttctc
    11281 ttgatattac gatatgctag gacaatgttg ttatcagaaa tgataatctg cattagattc
    11341 ttgaaattat ctcccttcaa acttttggag tatatcttat cgaagatttc cgtcatgcca
    11401 taatattctg catgccgcaa cttcctatct tttggacttg tttcttttgt catggtttat
    11461 cacgctccaa ttggctcgtg acctttttag tcatacgaga atcccatggt tgaacgggtt
    11521 tcatgacttt tacagacgaa tcgagcctgt tcctccagtt ccattacaga accttcatca
    11581 gtcgtgactc tactttcacc atcactaaag taagttattt gtcattcaca tatttcttac
    11641 taccacttca tcgagtatgc actctccatg ttgatggctt cccctgttcc cttatatcta
    11701 tccacatgcc ttaacttaga tgctagcttt tgccccctaa cttctcctac gcctgtaacg
    11761 cagcatggtc tttcataaca acgactttta ctcaaccaca agttagcaca atgagtgatt
    11821 ttttgctcac tgtctctccc atttctagga gctatgcctt tcggacagta cattcgctag
    11881 ttcgtcagta cgtttgtaca tactcaccat attaagtttt tttggacacc cgacttatcg
    11941 gatacaccct ttacctctag agaactttcg tggtattcac tatttctttt accttacggt
    12001 atccttcagc cgacttcacc gagcttcaca caacgttggt ctgcataaac tacttgttgc
    12061 atgtcggagt atcagtggaa gccctttgtg gcgttaccca ctcatttgac ttctcgttat
    12121 aagagttatt acaatagttt ttagactact tgcaacccac agcttttcac tgcagaacag
    12181 gtcgcaccat tatggcactc atcaatcatc gccaagaagc atttgaagtc ttgttcttcc
    12241 atttgttttt ctttaattaa acgtcgttgt cttcttcctt gaatcaatgc ctgattccaa
    12301 atcaacgtca tagccatgta catttgacat tgttgtaccc ctcgatctaa gcctgtaata
    12361 gagtcaatgt caaaaaagac caactgttgt tggtcaaggt gtcgaatcgt actatgacca
    12421 tcaaaaatca tgccatacgt ctcacataag gattctacga tggattccat ttcttctttt
    12481 cgattctttt tctcttggga agcacctggt aattccagtt gtcctaaaaa gtctaaaaat
    12541 tctttgaacg tcggataagc ttctggatct ttacctgtga cttgcgtatc agtatttgaa
    12601 cgcgaatttg aggaagtaaa gccgcgtggt actgttccat actcttcata aaaacgattt
    12661 aaataggtct taaattctgc tttcatcgtt ccttttagct gaggactcac taactgtacc
    12721 tgatagacca ctttgtctaa gtgcgtttga taggacgctg cttcattgat cttcaaatct
    12781 ttgactgatc catcgtcata taaactggaa gcttcatcat acgtacgtgt ggcaaatatt
    12841 tctaaaggat tgatcatccc gtcagatccg tctaaacgca ccataatgcc gttatattcc
    12901 tttaaaaagg gaataaagtc ttttcccttt tcaaagcccc aaataaagtc tcctgcagct
    12961 aaatgggctt ccgcgatcat tttaagaacg gtcgatttac cagctcccat cattcctaga
    13021 agcaaggtat tggtagacaa ccgcacacta tttttatacg tggcattcca aacaaacggg
    13081 ccgcctgtca tggtttcccc atattgtgtt ccacctggat cttttagcgt ttggtggtgg
    13141 aacgggaaac ctccgccaat gtttagcgat ggtagtgatt gccctttgat atttccaaac
    13201 cattcagctt gttcatcgaa catggtgaac atcgattgcc agtctctttt cgttttgtgc
    13261 agataggtca ctgcacgata atccatccct ttgagatttt cgatcagttc ctgtgctcgt
    13321 tcatctactt cttgaatcgt gtttccgtaa acatgtactc tcactttgac ttgtttagaa
    13381 atttccccat tagaagtcaa ttttcgggca tattccgcta gttctaggta ttcttccgcg
    13441 cttttatttc ggtcagtcgc ttttcgttca ttatctgacc gatcatttaa ttcatctaag
    13501 gcacgattga tttgatcaat cactttttgc ttttcatctg tgctaacatc tagtgttgcc
    13561 actgtatgag gattgttcat gagattggcc aaccaaaaaa gcgtaggatt ttttgtgtag
    13621 tgataaactg tcacacatcg tgaataacca tttccatagg tcacatactc ttcatcgaaa
    13681 tttaatccac cttgtggcat cacttgtttt aaaaagtcta aatcataccc tttggatttt
    13741 aaaatttcta tttcccgatt tcctagttgc tttaatggat caagtacctt ttgatcattt
    13801 tgtggtgaaa aagctgtttt aattgcttct aacatgtaca gtcacctctt cttgtttgat
    13861 tctttacttg gttaaaagat tttccttttc ccaacttacc caggcttaaa gtgtgaggaa
    13921 attttctagt ggtctcttct ttcggaacat ctttttctaa ccatatcaac ggatatttca
    13981 ttctctcttc cccattccta ctcatcattc atattattca ttcgataaag aatcttgatc
    14041 aattcttttt tgtagagtgg ttttaaccct aaaatgccac tagatcgcat taactgacgg
    14101 tttctggttt gcaaatcctt gattgtcttt ccttcaatga cgaaaaagaa gcttaaatca
    14161 gggcgttcct gttctgctcg tttcaattcc tggatttcct ctactaattt cttttcatat
    14221 tttcttaatc gctgtagatt tcttcctaag gactgttcca tacgattttt atctaataac
    14281 tgccgctttt gcacgatttg ttccgttaga tccgtctctg tggtcaaact aactaactta
    14341 attgttggca catacgtacg catgacggtg gtcaactgat tcattcgttc tagtttctct
    14401 tgttcattta atccttccac gtctgtagaa actacacgat aataacgttg aaaatgatct
    14461 tctctagtaa ttaagactcc ctcttctgta caggatttat acggaaataa gtccaagata
    14521 gagcgctctt ctggttttcc aaactgcaaa atggatttcc tttcaactga attttgtcct
    14581 ttgactcgca tttattcatc ctccttatac gtaccatgat aatttcggtt aggatctaac
    14641 gcatgataat ggttcttgtc cgcaaaaaaa atagactcga taatcgcaaa aatttgtcgt
    14701 tgacctgggt tattataggg tcgccaaaca atcactaacg ctatcgcaat ccataagcca
    14761 taattcaaga gtgcaaaaaa gagtggtaag tggacaagat tattgaccat tttcgcccaa
    14821 aaaaagcagc caccaagcgt taatagatct aaaaagaaaa aagcgaaaaa cttccattct
    14881 ccactaaatc ttggagggtt tttcatgttt atatatttat ccataattga tttctccttt
    14941 tcatataaag aaaaggccta acttgtaggc cttttatggt ttgatatcct gtttgatatc
    15001 cctcaatagt tgttctttca atgcattgtt cctgttttta gtttctcttg tcacatttat
    15061 ttttgttggt gcagtaaatg ccacatttgc tggcttgtaa cctgtatgaa cgttttgaat
    15121 aggttttggt gcagcttctc ttgtcacaac attttgaacg gacgcattcc ctgtctcacc
    15181 actttcagat aaattgtaag aaacagttgt cagatttcca ttttcaggca aacgacttgg
    15241 tttcatttga acagtacctg caggtgattg atgaacaaaa ctttgatcat tatccttgaa
    15301 ttgattcaaa cgactatctt tcattggtaa attttgtgcc ttaggtaaac caattttcgt
    15361 tgatttttca gttccaactt cgattccatt atcatttggc agaacgatgg aatcctcatc
    15421 cgtttgtact tcttgatttc gttttggtaa atctgaaatg cgtttttcat tttcggagtt
    15481 aacagatcta taccgatatt gttctggatc agtttttggt aactgtccct cattttctgc
    15541 tttgtcttct tgatttactt ttgataaatc tgaaattcgc ttctcatttt caaggtcact
    15601 gttaggttta tagcgatatt gttctggatc agtttttggt aactgtccat tttttccttc
    15661 ttctgaattt ctttcttcta tggaattttt ctcttcaatc gaattttcat tagagtgcaa
    15721 cgcttcttgg tgatctttga ctgaaggaat cactatacct tcttgatttt ctttttcggt
    15781 tgttgtatta gctttcactg ctgcattgac ttgtccttca ccatacgcat cttttagtcc
    15841 ttctttgact tctgaagctt tttctttttg tttttctagt gcttcggatg gattcttcac
    15901 agcttcagcc aattctttag ctctcgtgcc gtctaattta tcttctaagg tgtctttggc
    15961 tttttctgta agatcttcag ctgttccttt taacccacgt tcattgacgt aaccaagacc
    16021 ttctgcggct ttagaaacac catttttcgc tttcccgaac atttcggaaa ctgttttctt
    16081 actattaggt acagttgtag ctttatcgtc tgattgttct tccccgttat caggggtaac
    16141 atcttttcga tggccaaatc catttttacg aatatcattt aaatggctta aaccactctt
    16201 accagcactt gcaaggtttt tagctccgct acccacattt ttcgcaatat ttcctaaggc
    16261 aaacatgcta agaagagaat tactgccgtt tttcagactc gtatcaacgc cgaaaatttt
    16321 cgtaactgat tgagagcctt tgaatagcgc aatggttagt acaatcaaag cgacaacata
    16381 caagaatcca ttgatctttc ctgccgcatg caaatcacca atacctgaaa gcattaaggt
    16441 atagattcgt aattctaaaa ttgtaaatgc caataacaag aaggattgag aaatatcttc
    16501 gatagcactt ttcatccgtt gtcctgtctc aaagtccgtt gacgcggcaa tcactaaata
    16561 gaatttttta aataccagct caataatgca agtaacgatc gtaaatagga taaataaata
    16621 ggccaccgcc aaagaagatt caccagctaa aataatccct ggactactaa tgtatcgacg
    16681 ataccctgaa gtataaacat atttggctaa gccttcatca ctgatttgca caggaatttc
    16741 ttttccatta tcatccagtt ccagtcggta ttgtaacgct cttaaccgat ccttttgtaa
    16801 atctgataaa ccactcttat ctttatccgc catttcttta gcttgtttag gagtaataat
    16861 cccattgata ttcgctgttt taaaattttt cgctgttaaa gcatttcttt tgtctgttgg
    16921 cagggattct actttattcg ggtcagttgc tagtaatgta ctgacatcga tcacattatt
    16981 ttgaataatt tgaaaagcag gtgaacccgt tttatctgct ttatttgctg cagtgacgtc
    17041 tccgtaaaag cttttactga tatttaaccc ttcatcgatt aaagaactac caccaaaaat
    17101 caagatcatg gccataaata aattaaccaa gatatttttg acttttggtg ggtgtttact
    17161 cataaaaaat ttcaagccta aataaattaa tgtgacgaca caaaaaagag ccaatacctt
    17221 actaatcaaa ctttgaaata gttcatttac cccagcggcg ttcaataagt cttttaaatc
    17281 aaagctttga taaatggttt tttctagtaa agtattgaga ttatataacc ctttgatcaa
    17341 cccccataaa aaagtattac aaatccaact gaacacatct tgatactgca gataattacc
    17401 ataatcttgt aagactttcg ttacggtttt ggggtttgca tccccttctc cgaataagcc
    17461 tgaccaccag tcggctatgc cagaaaaaat tcctccaatg atcattcttt caccaccttt
    17521 tcagattcca ataacaaccg tttcccaatc atttcagaaa tttcttcatt tttgatcaac
    17581 ttttcaacat attcttgaag tcgatctgct gcttgttctt cgtaaaattg aattaacaat
    17641 tttttagccg tctcattctc aagagattga acgattttat aaaacatatt ttcttcgttt
    17701 ttttcttcta attgatcatg cag
//
LOCUS       DACECJ010000059        17250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.059, whole genome shotgun sequence.
ACCESSION   DACECJ010000059 DACECJ010000000
VERSION     DACECJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.059"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..1124)
                     /locus_tag="IWO09_001989"
     CDS             complement(105..1124)
                     /locus_tag="IWO09_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0264575.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1146..2153)
                     /locus_tag="IWO09_001990"
     CDS             complement(1146..2153)
                     /locus_tag="IWO09_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0264576.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="IWO09_001991"
     CDS             complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="IWO09_001991"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0264577.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(2804..5098)
                     /locus_tag="IWO09_001992"
     CDS             complement(2804..5098)
                     /locus_tag="IWO09_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0264578.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5358..7523
                     /locus_tag="IWO09_001993"
     CDS             5358..7523
                     /locus_tag="IWO09_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0264579.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7827..8660
                     /locus_tag="IWO09_001994"
     CDS             7827..8660
                     /locus_tag="IWO09_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0264580.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8762..10462)
                     /locus_tag="IWO09_001995"
     CDS             complement(8762..10462)
                     /locus_tag="IWO09_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264581.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10969..12129
                     /locus_tag="IWO09_001996"
     CDS             10969..12129
                     /locus_tag="IWO09_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0264582.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12145..12588
                     /locus_tag="IWO09_001997"
     CDS             12145..12588
                     /locus_tag="IWO09_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0264583.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13071..13601)
                     /locus_tag="IWO09_001998"
     CDS             complement(13071..13601)
                     /locus_tag="IWO09_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0264584.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13706..14257
                     /locus_tag="IWO09_001999"
     CDS             13706..14257
                     /locus_tag="IWO09_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0264585.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14410..14883)
                     /locus_tag="IWO09_002000"
     CDS             complement(14410..14883)
                     /locus_tag="IWO09_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0264586.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15021..16493
                     /locus_tag="IWO09_002001"
     CDS             15021..16493
                     /locus_tag="IWO09_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0264587.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16490..17200
                     /locus_tag="IWO09_002002"
     CDS             16490..17200
                     /locus_tag="IWO09_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0264588.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5363 a         3103 c         3547 g         5237 t
ORIGIN      
        1 cacgctcttt tttacagata aacaatgtgt tagaagtagt atcttctttt acataaaaag
       61 ctgaccatag gataaatggt cagcgtattt agtgagtcag ctatttagta tgggttttct
      121 aaaggaacga ctgtctctct tttgatcaag cgatgaggga tgaccaaacg tacgccactg
      181 ctcaattctt catggatcaa ttcgcgcagc ttttgcatag cgacacgtcc taattcggag
      241 atatcgatat cgatacttgt aaggtaaggg tgtgttaatg ttgagaagat cgagttgttg
      301 aagctaatga cggagagtgt ctctggaatg acatagccgt acatttgcgc caactgcatc
      361 gtgcgaaggg cgaagatatc gtcaatgacc accaatgctg tggcttcgtt ttctttcaac
      421 gttttctcaa aggacagata atcctccgga tgctctagta ctacactgga taacgcagga
      481 agaccagcca tcatcattgc tttttgataa ccgaaatatc gttcgaaata caaactttca
      541 tgagtcgtgt ttgtcacaaa caggatattc ttatgtccat tctggatcaa aaattctgtt
      601 gcttgtttac ctagaagctg gttatcatta tctacataaa cgatttcgtt ctcatgatga
      661 taaggctgtc cgatcaaagt gaaagggatg tggttttggt ataaataatc aatgacagga
      721 tcttgactat ctgaatacac tagaataaaa ccatctactt gtttttgtaa atgcatacgc
      781 tggacgttct ctaacagcaa ttcaaaattt ttagcagtag cgattgctgt agtcatatca
      841 taattccgcg cttcttcgtt gattgcttcc atgatctcaa gataaaatgg gttaccgatc
      901 cgctcttttg aatcaagagg gggtaagatt acgccgatgg cactggaaat gcgttttcct
      961 agattgcggg cagtcatgtt aggaacataa cctagttcat ccataatttt tcggactttc
     1021 ttctttgttg tctcggaaat actaggatga tcgttgatga cacgagagac ggtcgaggtg
     1081 gcaacaccgg cttttttcgc cacatctttt acggtaatag ccatgataaa aacacctttc
     1141 tcttgttatt gtgcagagaa ccgataaatc gtctgactga tccgttcttg accaggatgg
     1201 aggttgatcg atccaaattt ggggaaatga acgatatcag ggatttcttg cggttcgatt
     1261 gctaatccgt agtttgaatg catctgtttt ccattgacag agaaatcagc ttcgaatcca
     1321 gtcgtagaaa ataataccat acttttatta gaagttgcta tagtcatttg gcgaccagaa
     1381 tgcttctcga ataattgtaa ggaagttttg gaaagcctag gatgatgcaa aagaaataca
     1441 tcatctagtc catctggata acatgcaagg atctcttgca gtgttgggga ttttctaaag
     1501 tcgattggca gtttttcttt ttttgtccaa tttccagtag gcagtttttc ttgatctaat
     1561 tcaagcacac catctaaaaa gaccgttaac tgatgttcat agatgtctct tgttccattt
     1621 cctgaaaggt tgaagtaggc atggttggtc ggattgcaga tggtttcaac aaggctacta
     1681 gcagaggttg tcatctctaa ttcgtttttt gttaaacggt aagtgatcgt agcagtgatt
     1741 ggtcctggat aggtagaaaa ttcatctttt aagtaaaaga ccacacctat tgatgatgga
     1801 gttttgaaaa cttctacttc ccaaaattgg aaagaccagc ctttcgtgcc accgtggata
     1861 tggtgtgatc cgcagttttt ctccaatcta tgctcacccc aagaagcatc ccggatcctt
     1921 ccggctacag gtccgacagt tgctccaaaa aagcttttgt ctgttagaac atcttcgaaa
     1981 cgatcgtaag acaataaaac attttctgat tttccttctt tgtctggtgc gaagatttgg
     2041 tgcaggcggg caccgtaatt gagaagaacg acttctaagt agtcgttctt caatgtgatt
     2101 ttctttattt cagtctctgg aactggtacg acggagatct ctggattatc catggtcatg
     2161 acacttttct ttcaagtaat cgatcgtaag aaaagtcgta tcagggacaa gcgggatctc
     2221 ctctccaagg tcttcagcac gtcccacgcc aattggttga gcgccacttg agaggatggc
     2281 ttggataccg gcttgcgcgt cttcgatccc gatacattct tctgctttta gtccgacagc
     2341 ttttgctgca aggaggaaga tatctggcgc aggttttccg tttgttgctt ctgccgggtc
     2401 ggcaatagca tcaaagtaag gtgtaagacc cattttttca agtaggaatg gcccgttttt
     2461 acttgcagaa gcaagagcaa tcttgatttt ttcttctttt aaagaatcca gtaaaggcac
     2521 gaccccgggg aagacatctt ttggttcgat cgtctggatc atctctaaat aataatcgtt
     2581 ttttcttttc gctaattctg caaattcttc ctttgaaaaa gagtgttctt ttccgccata
     2641 agctaaaagc aaagagagtg agtcttctcg gctgacgcct tttagctgtt cgttgaatgt
     2701 gtggtcgatc gtaatgccga tttcctgacc aagctttcgc caagcacgat aatggaattc
     2761 agcagtatcg gtaatgacac cgtctaaatc aaacaatacc cctttaaaca tttgcttcct
     2821 cctttcttag cgttatttct agacgatccg ctaaagaata agactgttca tagacgtaaa
     2881 tttctagagg aaaaccttct aataactcga aagtaacttg ttcatctgtg actgagatga
     2941 acaataaacg tccgcgataa ttgatatgga aagaataagc tttccaagtt tttggcagaa
     3001 atggtgcgaa tctcaactga tcatgggcag ttttcatttg agcaaatcct tggacaatcg
     3061 acaaccagct accagtcatg gaagtgatgt gcaagccgtc ttctgtgtcg ttattgtaat
     3121 tatccaaatc caagcgagct gttcgctggt acatttctac agctttgctt tccattccta
     3181 attctgctgc taaaatcgaa tggatacttg gcgaaaggga agactcatgg acagtcattg
     3241 gttcatagaa atcaaagttt ttttgctttt cttcttttgt aaaagtatca ttgaagaaat
     3301 aaatcccctg tagtacatct gcctgcttga tgaagcaaga tctaaggatt ttgtcccaag
     3361 accaatgctg attcagagga agatcgcttg tgggaatatc ggatacaggc atgagatctt
     3421 tatctaagaa tgtgtcatgc tggacaaaga ttcctaattc ttcgtcaact gggaagtaca
     3481 tgttctctgc gatatctttc catttggcta gttcttcttc gctgatagtg atagcagctt
     3541 ctttttgata agttaaataa ttttctcgcg tgtaacgcaa gacccaagct gcgatcgtat
     3601 ttgtatacca attgttattg atattgttct catattcgtt tgggccggtc acaccgtgga
     3661 tcatgtattt atttgctcgt tttgaaaaat ggacacggtc tgcccagaag cgtgcgattt
     3721 ctgctaaaac ttccagtcct tcatttttca aatattccgt atcgccggtg taattcgtat
     3781 aattgtagat tgcataggca atcgcgccat ttcggtgaat ctcctcgaat gtgatttccc
     3841 actcgttatg gcattcgaca ccagtgaagg tgaccatagg ataaagcgcg ccttttaatc
     3901 cttgttgctg agcgttgtgg attgcttgcg gcagttgatt atgccgatat ttcagcagat
     3961 tcttagttac ttcaggtttg gctaaagcaa gataaagagg gacagcatac gcttctgtat
     4021 cccagtaagt ggctccgccg tatttttctc ctgtgaatcc tttggggccg atattcaatc
     4081 gttcatcttc gccgtagtaa gtagagaata gttggaacaa attgaatcga ataccttgtt
     4141 gtgcttcttc gtctccttcg atgaccacat cggcaagctt ccatcgttct gcccaagccg
     4201 ctgtttgctc ttcttttgct tggatataat tgtttgtctg gttcgttaat tctgagacag
     4261 ctttttctgc ttgctgcgct tctggaatat ctctactagt aagaacgata atttcttttt
     4321 ccactgacca tgtctcacct tgagataatt ctgcagacaa gcaaccatgt aatgctaaag
     4381 tatcttcgtg atagtcaggg atgactgatt ttccattaac gaaatggcgc attgctgcag
     4441 taacagtaaa ttgttcaatc ccaaagttgt ttggaatagt tttagtcgtc aaatagccga
     4501 tttcttctat agttccacga tcaatctcat tccaaaaatg ttcatcatag ttgctgtctt
     4561 cgttttgaac atttccatcc aattttgata cgagtttgac tgttccgcta ccttcaagca
     4621 tttctatagt taaatagata taagctgatt cttttttgac aatgctcaga aaacgttcga
     4681 aggaaatgcg tactttatta gtagaagtag tgattgtgaa tgtacgagat aatacaccat
     4741 ttttcatatc taattcccag taaaagtctt ctggttccaa agaagccaag tcgatcagct
     4801 gatcgttgat cgaaatatct atcgcgataa agttcaaggc gttgatcact tttccaaagt
     4861 attctggata accatttttc caccagccga cacgtgtctt atcgggatac cagacacccg
     4921 ccaaataagt accttgatgg tggtcccctg agtaagtttc ttcaaagttc ccgcgcatcc
     4981 ccatataacc gtttccaata cttgttaaag attcttgcag tcgaagattt tttttatcta
     5041 attgtgtcgt gctgattttc catggatcaa tagcaaatag tcgtttgatc tgtttcattt
     5101 ttctcctcct aatgagctgt tttatacgtt cataataaac gcaaccgatt gcacaagtca
     5161 atagacaaaa gaaaatattt catagaaaaa agcttaaaaa aataaatttt taggagaaaa
     5221 ttatagaagg gaacacacgt attataggtt ttatagaatg gtatagacag aaaaataaaa
     5281 aataaagcat ttacaaaaaa gaaaacgcac gctataatac gtttaacgaa accgattgcg
     5341 taaaggagct aaacggaatg aaaaagatat ttagttttga gttttggcaa aaatttggaa
     5401 aggctttaat ggttgtggta gcagttatgc cggcagctgg gttgatgatc agtataggga
     5461 aatcaattcc cttgatcaat cctgattgga cgccgcttgt cacaacgggc ggagtaattg
     5521 aaaatattgg ttgggcaatt attggtaatc ttcatatttt atttgcttta gcaataggag
     5581 gaagttgggc aaaagaaaga gcaggcggag catttgcagc tgggatctca tttattttaa
     5641 tcaatcggat cactggggct atctttggtg tcacaagtga catgcttgcg aatgaagatg
     5701 catttaccca cactctgttc ggtacgaaga tcatggttaa aggatttttt acaagtgtat
     5761 tggaagcacc agctttgaat atgggagtat tcgtagggat catcgctgga tttgtcgggg
     5821 caatggctta taataagtac tataattata gaaaattgcc ggatgctttg tcatttttca
     5881 atggaaagcg ctttgttccg tttgttgtta tcttatggtc aacaatcgtg gcattgattc
     5941 ttgctgtggt gtggccaaac gtacaggctg gaatcaataa ttttggttta tggattgcag
     6001 aatcacaaga aagcgcaccg attctagcac cgtttttatt tggaacatta gaacgtttac
     6061 tattgccatt tggattgcat catatgctga cgattcctat caactataca cagcttggcg
     6121 gatcgtatga gattttatca ggcgcacagg cggggacaca agtatttggg caagatccgt
     6181 tatggttggc ttgggccact gatttagtga atcttaaagg tgcaggggat atgtcgcaat
     6241 atgagtttgt gttgcataac tggacgccgg ctcgtttcaa agtaggacaa atgattggtt
     6301 cttcaggtat cttgatggga atggcttttg cgatgtatcg taatgtcgat cctgataaga
     6361 aagcaagata taaatcaatg tatttttcag cagcgttggc tgtctttcta acaggtgtaa
     6421 cagaaccatt ggagttcatg tttatgtttg ctgctgtgcc attatatgtc atttattcag
     6481 tgatccaagg ggctgcattt gcgatggcag atatcttgcc attgcgggtc cattcatttg
     6541 ggaatatcga attgctgact cggacaccgt tagctatcaa agcgggtcta ggtggagatc
     6601 ttatcaactt cgtgttgtgt gtgatcgctt ttggtgttgt tacgtatttc ttagcaaatt
     6661 tcttgatcaa aaaattcaac tttgctacac cgggacgtaa cggaaattac gacaatgaca
     6721 gtgaagaaac cgttgtatct aacagcggaa caggaacagc agatcagcaa gtggtccaga
     6781 tcattcattt gctgggagga aaagaaaata tcaaagatgt cgatgcttgt atgactcggt
     6841 tacgcgtaag tgtaaatgat cgagaaaaag tagggactga agaagcctgg aaacgagcag
     6901 gggcgatggg attgatcgtc aaagacaatg gagtccaagc agtttatgga ccaaaagcag
     6961 atgtcttgaa atcagatatc gaagatctgc tgcaatcagg agcagagatt ccagagccag
     7021 aaatggtttc tacaggaaat ggacaagcag gtagtaagca atacttaggt atagaacaag
     7081 aactagtttc cgcagcttct ggagaagtga ttcctttatc tgaagtaaaa gatccggtat
     7141 tctcacaaaa aatgatgggt gacggctttg ctgttattcc aacatcaaga gaagttgttg
     7201 cgccgatcgc tggaaaagtg acaagtattt tccctagcaa acatgcaatt gggctggaaa
     7261 cgaaagatgg tatcgaagtt ttgatccata tgggaatcga tacagtccaa atgaatcagc
     7321 cagcttttga gattgctgtc aaagaaggcc aagaagtagg aacaggaacg aaattagctg
     7381 agatggatct tgatgtgatc caaaacgaag aaaaagatcc aacgatcatg atcgtattta
     7441 caaacgataa agtagaagaa gtagtgatca agcaattagg tacagtgaca gctggtaaag
     7501 tgatcggctc cattaaaatg taaggagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     7561 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgag
     7621 aagcagcagt ttgaatactg tttagtggag aggtcgtgaa aaagctgccc tgcaccaagt
     7681 gataaaaaca gccaaacaaa aaatagctcc tggtccattg agcttattac tgaggagtta
     7741 gcaggagaac actatttgct ggagaggttg tgacaaaaat tttgtcacaa cccttttgtt
     7801 tcgaaatgaa gacaaggaga aaaccaatga aattcatgac actcaacacg catagctggc
     7861 ttgagccaga accagagaaa aaattgcaag aattagcaga taagatcctg ttagaagatt
     7921 atgaagtgat tgctttacaa gaaatcaacc aattactgga atctgaagaa gtagaacctg
     7981 cgaagctaat gaagttctgt caggtaaaga atcaagtacc tatccgtaaa gataattttg
     8041 cctaccgttt agttcagctt ttaaaggaac acggaaaaga gtatttttgg agttgggaaa
     8101 tgagccacat cggttatgat aaatatgaag aaggaaatgc actgcttacg aaaaaaacat
     8161 tggaaagtca ggtccttacc gtctcccaat ctcaaaagaa agaagattat cagacacgca
     8221 agatcttgat tgggaaaaca aagatagatg atcaggacat ttttgtctcg agctgccact
     8281 tttcttggtg gacagataaa aaaagtggct tttattttga gtggaaaaac ttggaaaact
     8341 actttttgga aactcgcgta ccactttttt tcctaggtga tttcaataat ccagtagact
     8401 ctcaaggtta ttatactgta agagaaagtt gccttcttct tcaagattcg tatgttgtcg
     8461 cgaatgaaaa aggaaaagca gctactgtgg aaaagaaaat agatggttgg gaacaaaata
     8521 cagaaaaatt gcgtatagat tttattttcg tccctgaagg aatgcaggtt aaaaaatatc
     8581 aacggatttt tgatgggatc gatagtccaa tcatcagtga tcactacggg gtggaaatcg
     8641 aattagatgt gaacgaataa aaaatataac atataacata aaaaaactga ttaaaataaa
     8701 actctccaaa cgattatacc gtttggagag ttttttgatc ggatgtattt atcccttttg
     8761 cctaagcacg taaaagcttg tatctatgat aaataaataa acatagcaag gtaaaccagc
     8821 caatcaaagt aagagaaaga atgaaataga aatagattgg cgtttgataa tgaagcgtga
     8881 gctcgttgat tccttttttc tgcttcacga ttggggtccc gatatctgtt acttcataat
     8941 cagtcaatgt ttttccattt tgctgtagca cagtccgatc gtagacgata acagggatat
     9001 gaacagtttt gtccccagtg tttttccact tgattgtcaa aagaccgcct ggatgtgttt
     9061 ttataaaagt tgtttcgttt tctagtattt cttcgttata ccggtcgtat ttattgtctt
     9121 ttgtctcctt atagattggc agatagtcag gtgtggtttt ttggaaaacg aacaagctgg
     9181 tagacatatc tttgctgaag aatgttttcc ttgcttcttc cggcgtatcg aatagataag
     9241 tatgtcgact gacaaatgtt tcattttccc acttttccag atgctggtat aagttctgga
     9301 tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa aagacggaac cacttttgtt
     9361 cagaccactg gttcagtgaa agggcaaggt aaagtagaag caatagcgta aatggcacga
     9421 aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac atttttttcg atcaatgtcg
     9481 tccatggaat caagctagta gaaagaaaaa agaaaacaag acttgtgtac aacacgattt
     9541 tttggaataa ggtgttcttt tgttgaaaga aaataaaaag caaaaaacct gataatatca
     9601 atataggaaa ggccttaggg taatggagcc aataactgcc atccagatct atcgcctttt
     9661 gactcatttc tctgttaaca aagggaggaa gaatcttatt cccactgtaa acaatagcta
     9721 atcctgccca aatatttgct gtcagaacaa aataaagcaa gaccgactta atcagttgaa
     9781 gcagtgcttt tcctttttca gtctggttaa aaaagtaata aagataaaaa gggatataca
     9841 tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt cataagtccc actgagacag
     9901 cgactttgat aacagggaac tttttatgaa ggacgaaatc agcaataggg atcatacata
     9961 aaggataaaa acaagtcccc cagctagtga atccttggtt gatagaccag tattgaatag
    10021 aatacgtact catgaataaa acggctactg gtacacttac ccaatttcga acattgacat
    10081 atctcagtaa aatcagcata gaaaatcctg aaataagata aagaatgaaa tttgaaagaa
    10141 tctgataatg aaaccagcta cctgcaagca acacgatcaa tccttgtaaa taagcgaaaa
    10201 atgggccgta aacggcattc acgatcctgc cactttgctg gaatccataa agggaaggaa
    10261 aataatggaa atccccatat ttaatttgtt gcgcggcatc atagaaccga ttgtaatgga
    10321 atggggcatc agcacccaaa attagtttat gtgtgaataa atgaggcatg agaaatgcca
    10381 aagcaatgaa aaaaattgct aaaaatgggc catatttttt caattgtcga actcctttgc
    10441 aaactttaga taaattcatc atatatccta tgcgagaggt aggcaagaaa ttagaaaatt
    10501 catgaaaata tttatgaatt ttacaagaaa aacataagtg aaagttctaa ggagagagga
    10561 tgcgacataa gttttgttgc atctccttgt tttgaaacag aatttttctt aatactcgaa
    10621 acaaagtagc tttttcatga cctctcggta aacggtgttc aatcagctgc ttctcggtaa
    10681 taagctcact agcctagaaa atatgagaag ctgttttctg actagcgctc ttattactca
    10741 aagcataacg cagttttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    10801 gtaataagcc caacccaaca aaaaatatga gtagctattt ttttgggctg tacttattac
    10861 ttgatgcagg acagcttttt cacaacctca tcggctttca ttttctattt ttttcctgaa
    10921 cccatataat aaaagaaaga acaaaaaaac ttgcaaggga ggacacatgt gaagcagagt
    10981 aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt tattaatatg tatcgtcgta
    11041 gggttactga tcgctttata tccattttat gtggattctt taaatagttt tattgatcaa
    11101 aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa ttgaagtgca gcgaaagaaa
    11161 atggaggaga aaaaacaacg attaactgac caaggattca atcctggtac tgatcctttc
    11221 aatgaaaaga acagaaatga aagtacaaca tccagccaat tagaggaatg gctgatcgga
    11281 agtatcaata tacctaagat ccaattaaat atttcacttt atgatcggct caatggtagg
    11341 atccttgaaa atggagcagg cgtcttgcaa ggaacctctt tcccgcttgg tggaaattcg
    11401 acgcattcag tgatctctgc tcacagcggt ttacctaatc gacggttatt taccgaatta
    11461 gatcgtttag aaaacggaga cacgtttatt ttgactgttt tgggggagaa attggcttat
    11521 caagttaaaa atatacaagt ggtactgcca gatgacacgt ctgttctaac gatttcagaa
    11581 ggagaagact tagttacctt gcttacatgt acgccgtata tgatcaacac gcatcgtttg
    11641 ctagtgacag gacatcggat tccttataac gaatcggtaa aaaaagaaga aggaaaagga
    11701 aatcaggaaa gaaccatgcg gcaatggttt attcttgcag gaacgataat agctgtagtc
    11761 atactgttag tgtttatcgg acgactaatc tatcagtata gattaagtaa aaaagtgctt
    11821 gatttttcat ttattattag tgattcgtca gggaaaccgg taaatggtag ttcattcatt
    11881 ttgaaacata agaaaaaaac acttactaga aatggtgtgc cgttttcagt acaaagcgac
    11941 catcatggga aagtcaagct ggatcaattg cctggtggaa cttatcgaat cgtttcagct
    12001 gatgatccta aagttgctgt ttcttttggt attagaaaag taaaacaaga gaagatatac
    12061 ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa atggttttta ttttaaaatc
    12121 aatgaataag taaggagaga aaaaatgatc cgcacaacaa caaaattgtc tgccaaagaa
    12181 ctggaatctg ttctatcgat ttggctgcat gctaacttgg attctcatga attcattcca
    12241 tcaacttatt gggagaatca agtagaatct gttaggaaag ctttaccaga tgcagtgatt
    12301 tacttagcag aaatagatca agaaattatt ggttttgctg gggtgattga tcagtttatc
    12361 gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa tcggtacgca actaatgaaa
    12421 aaaataaaag aaaccaataa aacactgact ttgacagtct atcaaaaaaa tcaatcagct
    12481 attcatttct atctttcaca aggttttaca attcaaacac agcaaaaaga tgaagcagta
    12541 aatgaggagg aacttgtgat gaaatggaat caaaacgctg atttttgatg aacagaataa
    12601 cgaaataatg ctcccagcct aattttttgc tgggagcatt atttcatttt gaagtcacta
    12661 atttaactgt gtgaatatgc agttgttaca atctttgata aacggtgttc aatcagctga
    12721 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttgtttctc
    12781 taatactcag gtcaaaacgc tgatttcaca acctcttgat aaacggtgtt caaacggctg
    12841 cttctcggta ataagcccac tagcctagaa aatatgaaaa gctattttct aactagcgtt
    12901 cttattactc aaagcataac gctgatttca caacctcttg ataaacggtg ttcaatcagc
    12961 tgaccctcgg tattagctca aaaaacggca aaacatgagg aactattttt ccgtttcttt
    13021 ctctaatact caggtcagaa cgctgatttc acaacctctc tttcacaacc ttattttact
    13081 cgtttataat tttcatcgaa gtattttccg ctacggatat catctggcat tccatcgtat
    13141 ggttcttcgc taataacatc ttcattgtct atacctgcat cacccatata agtgatcgta
    13201 gcaaattcag gaacaagtaa ttttggataa gtttcatatt tttcacgagc tgcttccatt
    13261 tctgcgatat gttcgttttg gaaagtaaca gtgatttctg gatgttcttc aacccattta
    13321 gggaaaaaga tcattccgtg aagtttcttt tcatttggca taaagtccaa tgccacgtct
    13381 gtccctgttg gctctgtcca agtgattttg aatacgccgt ccgtcaattt aacgatatca
    13441 gcttcttggt cttttaccca acggcctttg accattccgc catggatacg gtaatcaaca
    13501 gtatggtcat ttttggcata ccattcatat tcccaaccat tgtcatacgt atagataaag
    13561 tgagttccaa taaaatcttc caattcttta aattctttca tgtttaagca tctccttata
    13621 aattaaaaca tgtaataaac tacatgtttt cgattactca tttatgatag aatggattta
    13681 aagatttgtc aaggagggaa aactgatggc aagaaaaaat acacttcgat atattttgtt
    13741 agggttatta agtaagaaga agatgacagg atatgaattg aatcaatcgt tcaaaaatga
    13801 aataggagaa ttttggcaag caaaacatag tcagatttat cctgaacttg caaaaatgga
    13861 agagcaagga atcattcagc atcaagtaga gatcacaggt gaaaaattgg aaaagaaagt
    13921 ttatgaatta acagaagaag ggaaggaaca attggcagag tggattcata caccaaccaa
    13981 tgaactaccg gttaatcgag acgaatttgt tctgaaattg tatttcgtca aagacgtgaa
    14041 tgatccggca ttagtagaaa ttattcagca gcagcgtgac cttcatgaag aaaaacgact
    14101 tcatctatta gagcgaaaaa aaacgatctt tcctactgaa aaagaaaaac atgaaaatta
    14161 cggacactac ctgattttag cgcatgcgat caatcgggaa acagaatatg caaattggct
    14221 aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa aatatccgaa cagtgatgga
    14281 gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg gatattctta tgttcaaaca
    14341 ggcctttttt caagtattta tgtagcatct acttattaaa aagcttggta cttaggagat
    14401 tagtggcgtt tagagttctt ttaacaactg ttcgtagtca gctttttcat cgacaggaag
    14461 gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg atgtacatct tagcattctc
    14521 ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg tcgatcaatt cgatatcttt
    14581 gattgctttt gaaaaaactt tacggacatg gtgcattttc ttttggcgga tttctgcctt
    14641 ttctgcagct tttgacaaag taaatccgtc atctaaatat tttttgatca gttctacttt
    14701 tatgactgta ggtaaacgat attttctagg acaattcgca tcagcttcga tcgaacgaat
    14761 gaatcccttt tgttcccaat accttaattg acgtggagac acacctacga tatcacttaa
    14821 ttcactgatg ccaacaacca attgatcatt ttccaacaaa cgtttaaggg aagccccaac
    14881 catttttgtc actccttttg tcctcttgct tcccttaatt atatccgtat taacttatgt
    14941 gtcaagtagg ttgacaaata gcgaatttag gactaaagta ttctagtagt ttcagaaaaa
    15001 attcagaaag aaggagaaat atgagtaaca accaacccgt agatatttac ggaaaaccat
    15061 ataatcggac acttttagtt gttgtcttat taattggtac attttgtacc gttttgaatc
    15121 agaccttatt gacgacagct tttcctacat tgatgaaagc tttcgatatt tcagcgtctg
    15181 atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg gatcatgatc ccaataacag
    15241 catggctgat caataaattc agttcaagaa atttatatct ttctgccatg acgatctttt
    15301 tgatcggaac gattacttgc tttacagcac ctaattttag tacattacta atcggtcgct
    15361 tgatccaagc ttgcggcgtg ggagtatcga tgccattatt gcaaaatatc atgctatcta
    15421 ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc agggatcgtt attggacttg
    15481 ctcctgcttt aggccctaca ttatctggtt atatcattga taactaccat tggcgtgact
    15541 tgtttggtat ggtactgcca atcgttgttc ttgtcttggt tttagcattc ttcctaatga
    15601 aaagtgttat tccattgtca aatcctagca ttgatatttt atccgctatt ttatcaacga
    15661 ttggattcgg tagtttactt tacggatttt ctagtgtagg agatgacggc tggggaagct
    15721 tgaaagtaat tggtttctta gttgttggtg tgattttcat cgccttgttc tcatggcgtc
    15781 aattacattt ggaacatcca ttcttggaac ttcgtgtatt caaatcatct acattcacga
    15841 ttgcagctgt tttagctggt gtaacaaata tggcgatggt aggggcagaa atggtcgtac
    15901 cattgtatat tcaaaatatt cgtggagaat cagcattcca ttctggcttg atgttgttgc
    15961 cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg tgcgattttc gataaatacg
    16021 gcgcgaaacg tttagccatc acagggatgt tcattttgac agcggcaact gcaccatttg
    16081 ctttcttgac aaaaacgact ccagttttat atatcgtgat cctttatgcg atccgtatgt
    16141 ttgggatctc catggtcatg atgccggtaa cgacttctgg tatgaatgca ttgccaaata
    16201 acttgatcag tcatgggaca gcggtaaata atacattccg tcaaatcgca agctcgattg
    16261 gtacagctat tttgatcagt gtgttgacga atgtgacaaa agcaaatatc ccaagcaaat
    16321 ctgtattgga taatacgcca ttagcttata aagatcaggc aatcaatgcc acactgtcag
    16381 gttaccatgc cgcgtttatc gtagcgatca tctttgggat tcttggcttt attattgcct
    16441 ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg ggataatgca tgattattgg
    16501 agttttgatt gtcagcttgc ttgcttttgc tttaacgaac gtgttcgcta aaaaaacatg
    16561 gcaaaccttt ttatctttga ttttcggact aatcttcgtc gcttcactaa gtttgatcgt
    16621 cgctaatttg tccaatcact ttggaatgga aaaagtaacg gaaacaaaaa cagaaaaaat
    16681 tgtttccagt gctgattccc aaggagcaga tatgcttttg tataaagctt tagggaatgg
    16741 aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa gaaaaaccaa aagcaactgg
    16801 tacggataac gaaacaaata aagtagaaaa gacagacggc gatgctgaaa aagtgacgaa
    16861 aactacttat tgggaataca aaaacgacat gtataaattc tggttcaata ttgctgataa
    16921 caatcacgaa tatgacagcc gagtgaatac attcaaaatc ccagagacat gggtagaatt
    16981 aagtacggac caagcagcca agttagctga attagtgaag aaacagcaaa gtacgatgga
    17041 aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa gcaatgactg aaaatccaaa
    17101 aatgagcaaa gcagagcaag aacaacgtac gaaagcctta gctgctgaat tccaacaaca
    17161 agcattcgca aaattagtga aagaagcaaa aggcgaataa tattttgaac accatgtagg
    17221 aggttgtgaa aaagctgctc tgcatcaagc
//
LOCUS       DACECJ010000060        17224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.060, whole genome shotgun sequence.
ACCESSION   DACECJ010000060 DACECJ010000000
VERSION     DACECJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.060"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..369
                     /locus_tag="IWO09_002003"
     CDS             1..369
                     /locus_tag="IWO09_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0264589.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="IWO09_002004"
     CDS             549..1490
                     /locus_tag="IWO09_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0264590.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="IWO09_002005"
     CDS             1559..2290
                     /locus_tag="IWO09_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0264591.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="IWO09_002006"
     CDS             2434..4614
                     /locus_tag="IWO09_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0264592.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="IWO09_002007"
     CDS             4634..5116
                     /locus_tag="IWO09_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0264593.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="IWO09_002008"
     CDS             5132..5893
                     /locus_tag="IWO09_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0264594.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="IWO09_002009"
     CDS             6024..7358
                     /locus_tag="IWO09_002009"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0264595.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="IWO09_002010"
     CDS             7665..8150
                     /locus_tag="IWO09_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0264596.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="IWO09_002011"
     CDS             8143..8826
                     /locus_tag="IWO09_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0264597.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="IWO09_002012"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="IWO09_002012"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0264598.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="IWO09_002013"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="IWO09_002013"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0264599.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="IWO09_002014"
     CDS             12442..13071
                     /locus_tag="IWO09_002014"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0264600.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="IWO09_002015"
     CDS             13123..13455
                     /locus_tag="IWO09_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0264601.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="IWO09_002016"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="IWO09_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0264602.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="IWO09_002017"
     CDS             14281..15654
                     /locus_tag="IWO09_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0264603.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="IWO09_002018"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="IWO09_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0264604.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="IWO09_002019"
     CDS             16709..17149
                     /locus_tag="IWO09_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0264605.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3008 c         3795 g         4546 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggtagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggg
//
LOCUS       DACECJ010000061        16819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.061, whole genome shotgun sequence.
ACCESSION   DACECJ010000061 DACECJ010000000
VERSION     DACECJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.061"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..455)
                     /locus_tag="IWO09_002020"
     CDS             complement(<1..455)
                     /locus_tag="IWO09_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0264606.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(560..1423)
                     /locus_tag="IWO09_002021"
     CDS             complement(560..1423)
                     /locus_tag="IWO09_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0264607.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1619..3718)
                     /locus_tag="IWO09_002022"
     CDS             complement(1619..3718)
                     /locus_tag="IWO09_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0264608.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="IWO09_002023"
     CDS             complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="IWO09_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0264609.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5071..6912)
                     /locus_tag="IWO09_002024"
     CDS             complement(5071..6912)
                     /locus_tag="IWO09_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0264610.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7094..9487)
                     /locus_tag="IWO09_002025"
     CDS             complement(7094..9487)
                     /locus_tag="IWO09_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0264611.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9594..10454)
                     /locus_tag="IWO09_002026"
     CDS             complement(9594..10454)
                     /locus_tag="IWO09_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0264612.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10513..11034)
                     /locus_tag="IWO09_002027"
     CDS             complement(10513..11034)
                     /locus_tag="IWO09_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0264613.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11354..12073
                     /locus_tag="IWO09_002028"
     CDS             11354..12073
                     /locus_tag="IWO09_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0264614.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12099..14690
                     /gene="mprF"
                     /locus_tag="IWO09_002029"
     CDS             12099..14690
                     /gene="mprF"
                     /locus_tag="IWO09_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0264615.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="IWO09_002030"
     CDS             complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="IWO09_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0264616.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16433..16789
                     /locus_tag="IWO09_002031"
     CDS             16433..16789
                     /locus_tag="IWO09_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0264617.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5766 t
ORIGIN      
        1 ttttcttttt catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact
       61 aaaaaatgat aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact
      121 tcctcgatcg tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct
      181 tttctcaaat cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat
      241 tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt
      301 ttcactaaaa agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca
      361 agcagagtag cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt
      421 gtttttttca tcgccgaaat agcattcatg accatttact caaccccaac aatttataat
      481 aattacaaat aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag
      541 aaaaacatgc aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt
      601 gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga
      661 taccaaaagc tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat
      721 ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg
      781 ctcttggctt caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt
      841 ttatctgctc gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat
      901 agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca
      961 catctttgtt tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat
     1021 ctgctaaact gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat
     1081 cccctttttt aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt
     1141 ctttatctgg aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga
     1201 ttccaaaagc gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat
     1261 gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg
     1321 atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat
     1381 caataataat acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata
     1441 gtgaaaggtt ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata
     1501 gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc
     1561 ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt
     1621 aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg
     1681 gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat
     1741 tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga
     1801 tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc
     1861 atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg
     1921 ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg
     1981 cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt
     2041 cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat
     2101 cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat
     2161 ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca
     2221 ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat
     2281 attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg
     2341 tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat
     2401 cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt
     2461 ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata
     2521 aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt
     2581 gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt
     2641 ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc
     2701 ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct
     2761 tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat
     2821 ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt
     2881 tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg
     2941 attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt
     3001 ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata
     3061 cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg
     3121 tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt
     3181 tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg
     3241 tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga
     3301 cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat
     3361 tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta
     3421 tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata
     3481 attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta
     3541 attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat
     3601 ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt
     3661 ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca
     3721 cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg
     3781 tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat
     3841 agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac
     3901 agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt
     3961 gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac
     4021 gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat
     4081 ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat
     4141 cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct
     4201 ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg
     4261 aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt
     4321 ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga
     4381 tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt
     4441 ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact
     4501 taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt
     4561 ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta
     4621 aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca
     4681 atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat
     4741 acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg
     4801 cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat
     4861 ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat
     4921 tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg
     4981 gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat
     5041 ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa
     5101 attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc
     5161 ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc
     5221 tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc
     5281 caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa
     5341 ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg
     5401 aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg
     5461 atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt
     5521 ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt
     5581 tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt
     5641 tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac
     5701 tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt
     5761 taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa
     5821 agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac
     5881 gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc
     5941 aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg
     6001 ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc
     6061 ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg
     6121 gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt
     6181 ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca
     6241 gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg
     6301 ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa
     6361 caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg
     6421 tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata
     6481 ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc
     6541 tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt
     6601 acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc
     6661 ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc
     6721 acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc
     6781 ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg
     6841 tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat
     6901 ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta acagaagata
     6961 tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac
     7021 acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct
     7081 atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc
     7141 agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct
     7201 ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct acatatatcc
     7261 ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta
     7321 caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat
     7381 ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca
     7441 aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt
     7501 tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg
     7561 tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa
     7621 taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca
     7681 ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca
     7741 gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt
     7801 ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt
     7861 ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat
     7921 agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg
     7981 catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc
     8041 cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt
     8101 tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt
     8161 cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt
     8221 gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta
     8281 cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg
     8341 cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca
     8401 tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt
     8461 tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg
     8521 tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt
     8581 cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta
     8641 ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag
     8701 aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag
     8761 gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac
     8821 gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta
     8881 ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag
     8941 ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg
     9001 cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca
     9061 aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta
     9121 gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt
     9181 gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg
     9241 cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata
     9301 tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct
     9361 ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct
     9421 ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta
     9481 aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat
     9541 gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta
     9601 tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca
     9661 ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc
     9721 gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa
     9781 atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc
     9841 acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata
     9901 tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg
     9961 ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg
    10021 tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta
    10081 tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg
    10141 acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa
    10201 agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct
    10261 ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca
    10321 gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc
    10381 ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt
    10441 tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca tgattgaaca
    10501 tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt cattcatttt
    10561 gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag
    10621 caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct
    10681 cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt
    10741 tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca
    10801 tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact
    10861 gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag
    10921 atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt
    10981 ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt
    11041 taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat
    11101 catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta
    11161 ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt
    11221 tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg
    11281 atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac
    11341 agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat
    11401 ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag
    11461 cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt
    11521 agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat
    11581 ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg
    11641 atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca
    11701 tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt
    11761 catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg
    11821 caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt
    11881 aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa
    11941 acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca
    12001 aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct
    12061 ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat
    12121 ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta
    12181 ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt
    12241 ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt
    12301 tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc
    12361 gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg
    12421 ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg
    12481 tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc
    12541 tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga
    12601 tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa
    12661 agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat
    12721 tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc
    12781 ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt
    12841 ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta
    12901 ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca
    12961 tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc
    13021 agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg
    13081 tcttattata cttttctgga atcatgctag tactttctgc cactatcccg caagctttta
    13141 cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc
    13201 ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga
    13261 aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa
    13321 gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt
    13381 ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg
    13441 gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg
    13501 atttccctca tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt
    13561 ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt
    13621 tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga
    13681 tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga
    13741 tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata
    13801 ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg
    13861 aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg
    13921 aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag
    13981 cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg
    14041 tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta
    14101 ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa
    14161 aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg
    14221 tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca
    14281 ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta
    14341 tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca
    14401 atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa
    14461 ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag
    14521 aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat tctagagata
    14581 gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga
    14641 cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata
    14701 ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc
    14761 ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt
    14821 tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta
    14881 tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa
    14941 tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc
    15001 accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt
    15061 gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc
    15121 ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt
    15181 agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac
    15241 gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac
    15301 tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc
    15361 accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc
    15421 cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac
    15481 tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa
    15541 tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc
    15601 gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt
    15661 caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt
    15721 ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc
    15781 ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg
    15841 gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc
    15901 ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa
    15961 tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc
    16021 aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc
    16081 taaataatat agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg
    16141 actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat
    16201 acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta
    16261 ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc
    16321 ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt
    16381 tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa
    16441 attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca
    16501 ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc
    16561 tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa
    16621 taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat
    16681 gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat
    16741 aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt
    16801 aaaattttat tagataaaa
//
LOCUS       DACECJ010000062        16657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.062, whole genome shotgun sequence.
ACCESSION   DACECJ010000062 DACECJ010000000
VERSION     DACECJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.062"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(221..1177)
                     /locus_tag="IWO09_002032"
     CDS             complement(221..1177)
                     /locus_tag="IWO09_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0264618.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IWO09_002033"
     CDS             1281..1883
                     /locus_tag="IWO09_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264619.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILYFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IWO09_002034"
     CDS             complement(1971..3470)
                     /locus_tag="IWO09_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0264620.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IWO09_002035"
     CDS             complement(3486..4010)
                     /locus_tag="IWO09_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264621.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IWO09_002036"
     CDS             4308..5057
                     /locus_tag="IWO09_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0264622.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IWO09_002037"
     CDS             5048..5833
                     /locus_tag="IWO09_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0264623.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IWO09_002038"
     CDS             5802..6518
                     /locus_tag="IWO09_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0264624.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IWO09_002039"
     CDS             complement(6620..7639)
                     /locus_tag="IWO09_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0264625.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IWO09_002040"
     CDS             complement(7658..8716)
                     /locus_tag="IWO09_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0264626.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8936..10084)
                     /gene="nagA"
                     /locus_tag="IWO09_002041"
     CDS             complement(8936..10084)
                     /gene="nagA"
                     /locus_tag="IWO09_002041"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0264627.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10160..10996)
                     /locus_tag="IWO09_002042"
     CDS             complement(10160..10996)
                     /locus_tag="IWO09_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264628.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11180..12397)
                     /locus_tag="IWO09_002043"
     CDS             complement(11180..12397)
                     /locus_tag="IWO09_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0264629.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12780..12863
                     /locus_tag="IWO09_002044"
     tRNA            12780..12863
                     /locus_tag="IWO09_002044"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12814..12816,aa:Leu,seq:gag)
     gene            complement(13103..14473)
                     /locus_tag="IWO09_002045"
     CDS             complement(13103..14473)
                     /locus_tag="IWO09_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264630.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(15187..15477)
                     /locus_tag="IWO09_002046"
     CDS             complement(15187..15477)
                     /locus_tag="IWO09_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264631.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(15685..16377)
                     /locus_tag="IWO09_002047"
     CDS             complement(15685..16377)
                     /locus_tag="IWO09_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0264632.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         5051 a         3329 c         2780 g         5497 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgtattttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttgg
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttcgg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatggcgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccagcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaacctta aaaccaaact taagaagttt agttttaagg ttaacttaaa ttatattatg
     8941 cgtggaaacg ttccacgcca tctagataag ttgcttccaa tgtcatatca gggttcaata
     9001 cgataaagtc tgcatcgcgt ccttgagcga tttttccaca tgtatcatcg attttacagc
     9061 tgattgctgg aatcagacta gccatcatga ctgcttgttc tggtgttgct aatccccagt
     9121 ctacaacatt tttaattgct tctttcaatt tcaatatact tccagctaag ttgccagatt
     9181 ctagacgtgc tgttccttct tttactacta ctggaaattc gcctaaattg taatttccat
     9241 caggcatccc accggccatc atacagtctg taattaatgc gacatgatca tgtcctgctt
     9301 tttccattag gattcccgca gcatttggat gtacatgatg gccatcacaa atcaattcag
     9361 aaaagacatg ttctaaagat aataacgcac ccaccatacc tggttcgcga tgattcaatc
     9421 cacgcatacc attgaaagca tggacaaata cacttgcgcc tgcttcaaca gctttttgcg
     9481 cttcttctag tgtagcatta ctgtgtccta gagaaacaac tactccttct tcagtcactt
     9541 gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa agcaattttt ttgattaaac
     9601 cacctgaagc ttcttgccat tcatggaaag tatccaaatc aggatccccg aaataacttg
     9661 ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc ttcaaaatag attcctttga
     9721 ttttagctcc tggtgcttcc tgatacattt tgccgattgt ctcagctaca tcttttaaac
     9781 gttctttgct tgacgtcaat gttgttggta aaaatgaagt cactccacaa gataacagac
     9841 cttcagacat cactttgatg ccttctgcat cattatccat tacatcatga ttcatatagc
     9901 cgtgaatatg tgtatctacc aaaccaggag caatccattt gccgctttgg tcaatgatgt
     9961 tgacttctcc ctcaggtagt tctttggtgt attcaccaaa aagtccatcg gtaatttcaa
    10021 gatagcctgc atgtttgaca cctgacgcta aaaagaattt gtcagcatag ataaaagttc
    10081 tcattctatt ctctcccttt gtttttctac ctctaaagta ctcttattta gagaagaagt
    10141 caagaatggt ctacaccatt tacaaatttt ttgaaatttg tttcacagct tgtgctaagc
    10201 cttcatagaa tgctgctctt tctggttctt ctttacgctt gtatattaag cttttaagca
    10261 caaataaatc attgaggtaa tagtagctat tcttctcttg agcccaatag attccttgat
    10321 tcacctgttc taacgcttcg tcgatttcat tttgatggaa taagaaattt ccataattat
    10381 aaaaaatttg cgaaaattca ctttttgatc gttcatcagc tgattgatgc agcagttgga
    10441 tgcttcgact caaatagtag cgtgcttcag ccgtttttcc tgccacacca tatatttttc
    10501 cgatacaact aatcagttgt atttctgtgt cagaaacatg gattctttct ttttggctag
    10561 agtagcttaa tgcttctttc aaataataaa gggactgaga gatattttga cgcaacgtga
    10621 attcgcaaac accgagataa taataatagc gttgaaagtc agttgcttga tacagatttt
    10681 cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt ttttggaaac
    10741 aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga gagagattca
    10801 tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc aatacattcg
    10861 gcagttcctc attattttcg atccgactta atacactttg tgaacaaata tcatgcgaaa
    10921 gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt ccaaaggact
    10981 cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc tattcgtatt
    11041 ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata tactttagtt
    11101 atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg
    11161 aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct ctcattttct
    11221 tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa tgatctggct
    11281 ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca
    11341 aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt gggaatatat
    11401 aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt gcattataga
    11461 tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg acactttgat
    11521 aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac aaacgcatgg
    11581 aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct ccacttagga
    11641 ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg ttcagcgtaa
    11701 caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca tccatcacta
    11761 agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct acttcaacga
    11821 tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga ttaatgataa
    11881 caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca ggataccatt
    11941 ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca atagaagccg
    12001 tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc agcaacccct
    12061 gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca ttgatcgtca
    12121 cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca gaaaagatcc
    12181 ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca cgaatgactt
    12241 cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa
    12301 tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca tcgtaactga
    12361 caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc cctcatttca
    12421 aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata ctccatccta
    12481 gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa acaagcgttg
    12541 agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat tgcaattcca
    12601 caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc aatcaataca
    12661 cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt aataagtcct
    12721 cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt tattattacg
    12781 cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt tagctcgtgt
    12841 gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt cacgagtttt
    12901 tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt acaaaaagaa
    12961 cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa gctactcgta
    13021 gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc acctaatact
    13081 aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag gtacaaatac
    13141 caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg cttttcatta
    13201 gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata tagtacgtcc
    13261 gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa caaaatccca
    13321 ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt aaataaattc
    13381 caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa gtacctggat
    13441 aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta ctactactcc
    13501 aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt gtattaccaa
    13561 aataaataat caggtttaag aaaaacggta atgcagtcaa tatacgcaca taatagtttt
    13621 gacatttcct atatgttaga aggaaaatta agaaaaatag caataaaaat aagatattca
    13681 tgtctaaaaa aaacggcttt ccaaatgatg aaatacctaa atctaactta ttaagaaagt
    13741 tgaaattttt atattcagga aaccatttat taatttcatg tgcagcacgt aatgcattcc
    13801 ctggagttgt caaagagaaa attaattctg ctaagcttat tattgaaaat actgataact
    13861 tataattata tcgtccattg acaatcaaat ataaactaac tatacttgtt agaacaaaaa
    13921 aacaggcgtt gacttgttct tgattagtta aaaatatcaa taaaggaata cttacccaat
    13981 atatttttct atttatttct tctcctctta agagttgaaa aaacggataa aaagttagta
    14041 aactaaatgc tactggccac tgatagttca aggtggttgc tctccaaccg gtttccccca
    14101 ttactgtcaa tggtattaaa cagtatatag agcaaaccaa taataaattt ttaacattta
    14161 ttttctcaaa aacatacttg caaagtaata ctacaattcc taacataatc gtagaattta
    14221 ataatttcca tagcataaag tgtttagaaa agaacattaa gaatagttct ataataattc
    14281 ttgaactcca tgtcttataa tgttctatcg aagcacccaa atagccaagt tcatctaatc
    14341 tacttgcata aactacgtca tccgctctca tagtcggcat atacatatta aagagtaaaa
    14401 agatacccca aatccctatt agtaacaaga tcagttcttt gttattcgtt atttttctat
    14461 tatgtttgtt catttttata ctccttctta tatttcctaa tagagtataa catagcttta
    14521 agcattactt aaagataact taaaagttat cttttaaata cttaattgtt aaatacctta
    14581 tttgatgcaa aagaaatcta cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat
    14641 ttttcttagt taaggaccta tggattgcag taatgcagaa ttcgtcgaat aaactgccaa
    14701 agacatacaa tcatattgct tttaaaattg acgaacaaga tatagattcg tttgtctcaa
    14761 aaatccaaat gttaggttta actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt
    14821 cttcaattta cttttacgac tatgataatc acctcttcga gttacatacc ggtactctcc
    14881 aggaacgttt aaaatcctac aattccacta cctgagacta gtaatgacta gtctcttttt
    14941 atggcctctg atccccccat tgagcgaata ctaatagggc ttcgaattga tctgttcaat
    15001 ctttaagcaa tatccctcaa atgagtttgg aaagtttagt gattcatcac aaatacttaa
    15061 atacatgtag ttatatatag attgaggttc ttgtgagcgt atgctcgtgt gggttcaaat
    15121 cccatcgtct gcatccatct ttccaaaaca agcggctagg acatttttgt ctcagccgct
    15181 tgttttctag tttattcttt catcttgtcg gacctttttt cttgggtata gacttttctc
    15241 atattctacc acaattttgg gtttccctaa cacatgatag gatctcacaa caaattgctt
    15301 tctattatta taataacctg taacgactaa cttcatccct tcgtctactt cagctaggaa
    15361 gttcaaacta tgggaagcaa tcaaacaatt gaccccattt aaactgaaac ggatcaacgg
    15421 tctggcacta acttgcaaag tacggatctt atctactgtt ccatattttt tctccataac
    15481 cttcacctca ataccattat acgaacatac attccctttg taaagaagaa actctgtttt
    15541 atctttttga ctttttgtct attacctttc ttaaaatcat attggcactt cctgcgaaga
    15601 tgaaggacat gtactaaaaa aaagaggcag cagtatagat tactgttgct tctttactaa
    15661 tttaggttta cttgttaaga aagattattt acgcaaatgt tcttcaacaa tttgctgaga
    15721 tttatcagct gtatcttcca cttcatcttt tgctttttct aacttttgaa tatattgttt
    15781 caagcttgag ttttcagcag ttgcttcgtc taatttcttt tgtgtatctg ttaactgagt
    15841 ttctacattt gttttttctt gtgtcagtgt tgcgatttgt tgattcaaac catttacagt
    15901 attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt cttgctgtcc aatctgcatt
    15961 cagatgattg atttcatcat ttttttggtt gatctcatct agtttgacat tgatttcatt
    16021 ttgtttgctt tgtagttgtg catccttgct gactaattga ctttctaagt tttgtttttc
    16081 tgctgccgat tgattttttt ggttttctaa ttcatttact tttgtttggt attcttggac
    16141 atctgccttt gaatcttgca atgtctgttc tttttgtgct agtcgagcgg taagatctgt
    16201 aagcgcttgc tctttatctg tcaatgtatg atcaagctgt gtcagattct cattgattgt
    16261 atcaatatct gtctctccac cccacaaaat aatttcattt gcgtaccgcg tgcctgtgta
    16321 gccaattaga catagaaatg caacgacaat cccaccagta ataaatttct ttttcatttg
    16381 catagctcct taatccaata cttttacatg ctaaaagtaa gtgatctcaa acacaatgtc
    16441 aagcgttttt aaattacatg tgtaatttta atataaatac tatcaaatac tcatttaatc
    16501 ctcctttttt atctatttaa aaaatctttt ttatagagaa aaatgcaggt taatactttc
    16561 ttaaataaat atttatggtg aacaaaaaca aaaaagcaag ttttattaaa cgtaaccctt
    16621 ttcatttcat agattactgt atttattaca aaaaacg
//
LOCUS       DACECJ010000063        16605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.063, whole genome shotgun sequence.
ACCESSION   DACECJ010000063 DACECJ010000000
VERSION     DACECJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.063"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(81..1817)
                     /locus_tag="IWO09_002048"
     CDS             complement(81..1817)
                     /locus_tag="IWO09_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0264633.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1814..2518)
                     /locus_tag="IWO09_002049"
     CDS             complement(1814..2518)
                     /locus_tag="IWO09_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0264634.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2649..3152
                     /locus_tag="IWO09_002050"
     CDS             2649..3152
                     /locus_tag="IWO09_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0264635.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3112..3450
                     /locus_tag="IWO09_002051"
     CDS             3112..3450
                     /locus_tag="IWO09_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0264636.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3471..4889
                     /gene="ffh"
                     /locus_tag="IWO09_002052"
     CDS             3471..4889
                     /gene="ffh"
                     /locus_tag="IWO09_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0264637.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5001..5579
                     /locus_tag="IWO09_002053"
     CDS             5001..5579
                     /locus_tag="IWO09_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0264638.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5583..6104
                     /locus_tag="IWO09_002054"
     CDS             5583..6104
                     /locus_tag="IWO09_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0264639.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6183..6737)
                     /locus_tag="IWO09_002055"
     CDS             complement(6183..6737)
                     /locus_tag="IWO09_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0264640.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6990..7265
                     /gene="rpsP"
                     /locus_tag="IWO09_002056"
     CDS             6990..7265
                     /gene="rpsP"
                     /locus_tag="IWO09_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0264641.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7277..7528
                     /locus_tag="IWO09_002057"
     CDS             7277..7528
                     /locus_tag="IWO09_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0264642.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7653..8444
                     /locus_tag="IWO09_002058"
     CDS             7653..8444
                     /locus_tag="IWO09_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264643.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8614..9135
                     /gene="rimM"
                     /locus_tag="IWO09_002059"
     CDS             8614..9135
                     /gene="rimM"
                     /locus_tag="IWO09_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0264644.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9125..9886
                     /gene="trmD"
                     /locus_tag="IWO09_002060"
     CDS             9125..9886
                     /gene="trmD"
                     /locus_tag="IWO09_002060"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0264645.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            10022..10369
                     /gene="rplS"
                     /locus_tag="IWO09_002061"
     CDS             10022..10369
                     /gene="rplS"
                     /locus_tag="IWO09_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0264646.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(10501..11637)
                     /locus_tag="IWO09_002062"
     CDS             complement(10501..11637)
                     /locus_tag="IWO09_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0264647.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(11753..12667)
                     /locus_tag="IWO09_002063"
     CDS             complement(11753..12667)
                     /locus_tag="IWO09_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0264648.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(12758..13186)
                     /locus_tag="IWO09_002064"
     CDS             complement(12758..13186)
                     /locus_tag="IWO09_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0264649.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(13191..13565)
                     /locus_tag="IWO09_002065"
     CDS             complement(13191..13565)
                     /locus_tag="IWO09_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264650.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            13851..14036
                     /locus_tag="IWO09_002066"
     CDS             13851..14036
                     /locus_tag="IWO09_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264651.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            14048..14296
                     /locus_tag="IWO09_002067"
     CDS             14048..14296
                     /locus_tag="IWO09_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264652.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            14327..14506
                     /locus_tag="IWO09_002068"
     CDS             14327..14506
                     /locus_tag="IWO09_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264653.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(14549..15019)
                     /locus_tag="IWO09_002069"
     CDS             complement(14549..15019)
                     /locus_tag="IWO09_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264654.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            15106..15804
                     /locus_tag="IWO09_002070"
     CDS             15106..15804
                     /locus_tag="IWO09_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0264655.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            15982..16323
                     /locus_tag="IWO09_002071"
     CDS             15982..16323
                     /locus_tag="IWO09_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264656.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            16316..>16605
                     /locus_tag="IWO09_002072"
     CDS             16316..>16605
                     /locus_tag="IWO09_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0264657.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         5464 a         2900 c         3246 g         4995 t
ORIGIN      
        1 taaaaaagaa caaccactcg tgtatctttc gaagcaggat gagtcatcca actttaaaac
       61 gcacgagcag ttgctctttt ttacattttc ggaatggtaa cggtgaaagt cgaacctgtt
      121 ccaagatgac tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc ttttacaatc
      181 gctaacccta atccggtccc gcctgaatgt cggcttcgcg ccttgtctac gcggtaaaaa
      241 cgctcaaaga tccgttcctg atcctcaatg tcgataccta taccaaagtc ttggacagaa
      301 aacgatagat catctgttgc ctgatagcgg ataataatct cgctgtcgct ttttgaatat
      361 tggacagcat tttcaattag atttttgata attggataaa ggatatctgt tttagtagta
      421 aatggttcgt tctttccaat caatcgaatc gtcaaccgtt tcttttcgat gattggctga
      481 taattttgaa cgatttgctg gaaataaggt tctgtataaa ttgtctggat ctcgtaagaa
      541 ttctcgccat cttttgacag ctggatgatt tcccgaatca gtttatctaa ccggatcgca
      601 tctttttgca tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt tgcaccatcc
      661 agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag tttttaattc gtgagagacg
      721 ttgccaacga aatccttttg tagcttttct aaacgtcgca ctttcgttaa gtcatacgcc
      781 acccctagaa tctgtccggt accttctgaa ttagtaaagt agcgcaaact caagtctaat
      841 gtctgccgac cttctgtaag ggtgatctcc tgatggatca gcggatgctc aggagtcact
      901 tgatgaatca gctgaatgag ttttggctct tgaatgactt ctgtgtaccg ctgttcaggt
      961 tgatagctta ccacgtttaa atgtagctgc atctttggat taagcagtag taaacgagaa
     1021 tcagcatcat caatcaggaa tacgccaatc atcagttcgt tcagtaacgt atataattgc
     1081 tcttctgtgg tagtacacgc ccgatacatc ttactcattt gttcgctcaa cgcattaatc
     1141 gtctgataca attcttccca ttgatcgggt gtttgcatga ttacttccgc ctgattagga
     1201 ttttgcacca ttttttttag tacgggcagc acggttttca atggctggtt tttttgatag
     1261 atcagataat agatcattcc tgcaagaaca aggaagaaag acagaaagaa gaaaaagacc
     1321 caacggcgaa aactctcagt tctcggtaaa aaaccgcttg tcggttctgc gatacgtaaa
     1381 atggcttcta actggccact ttttttgact ggtaatgcga catataaaag atcctcattc
     1441 aaagtagtac ttttgcgcaa agcgctacct aggttcccac cattcaatac ggctttgatt
     1501 tcgggacggg agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa taagatcttt
     1561 ccagtagcat ccattaaagt aatccgttcg ttcgactgat ggacgaattc ttccaaagca
     1621 gttttgtttt gcgctgcttg gaggttttcg acatctaatt gccggatcag cagttgtgct
     1681 ttttttgtca ggtagctttc ttgctgttcc acgatctgct gttgaaaata atgggaaatc
     1741 aattgccagc caccaaaaaa caaactaact aaaagaataa gaaagaagag acgttcataa
     1801 atttttttcc atttcatcgt ttgggttcct gaaacttata gccaaatcct ctaatagtca
     1861 ccaagtactt tgggtgtttt ggatctcgct cgattttttc tcgcaaatga ctaacgtgta
     1921 catctacgat tcggctttgt cctgcgaagt caaaattcca aatgcggtct agcaatgtgt
     1981 ctcggtcgat cacacggtct tttcgtttca tgaaataaac aagtaattca aattctttcg
     2041 gtgttagttc aatcagatgg tcgtctacgg tcacttgata attggtcaaa tcagcagtga
     2101 tttgtccaat cgatagatat tcaggctcag cttcatcttt tgattgctta cgaggttcaa
     2161 ttcgtctgaa tattgctttc atacgggcta atacttcgcg aggactaaat ggtttggtca
     2221 aatagtcgtc agcacctatt tctaggccaa tgatccgatc cacttggtca tctttagccg
     2281 tcagcatcaa aatcggtgtt tcattttttt cttgacgcag ccgctgagtg atctccatgc
     2341 catccatcgt tggaagcatc acatctaaaa tgatgaaatc aaatgattgt tctaatgcta
     2401 attctaatcc ttcgcctcca tctgtggcac tggttacctt gtagccttct ttttccaaat
     2461 tgaacgtcaa gagcgttaca atagaaggtt catcatcaac aactagtatt tttttcattc
     2521 aaatctcctt taaataggtt tcattctgtt tatattttag catagaatca gtacaaaggg
     2581 ctattttaca aaagaaataa atggtataat aggaaggcac tttgctgaat agaaaggaag
     2641 agcagtcaat gattggaatc agtgcatgtt taggcggtct ggcctgtcgc tatgatggac
     2701 agaaaaaaga agtaccagaa ttgatggaac tagttgaaac tggagaagct gtcatggtct
     2761 gtccagaagt gatcggcgga ttaccaattc ctagagatcc cgctgaaatc attggtggaa
     2821 atggattcga tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc gaagatgtaa
     2881 cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa aaacacattg
     2941 atctagtcat catgaaggaa aaaagccctt catgcggaac ccattcgatt tatgacggaa
     3001 gtttttccgg aataaaaaaa aatggtgccg gagtggctac tgcttatttt attcagcaaa
     3061 aaatgactgt gctctctgat gaagaatggc tgaaaactag aggtgagcag aatggaaatc
     3121 gaaaagacca atcgaatgaa cgcgctcttt gaattttatt cgaccttatt gacagaaaaa
     3181 caaatgaact acatggaatt atattatgca gatgattttt cattaggtga gatcgcggaa
     3241 gaatacgaag taagccgaca agcagtatat gacaatatca agcggacaag caagatcttg
     3301 gaggattatg aaaaaaaact ccatcttttt tctaattata ttgttcgaga acaggtattg
     3361 gaaaatatga ctgcatatat tacagataag tacccggaag ataaaaaact acttgaatat
     3421 gtacagcaaa ttcaagcaat cgaagaataa ataaaaggaa tgaagaaaat atggcatttg
     3481 aaagcttaac tgaacgtctc cagcaggcga tgggaaaaat aagaaaaaaa ggaaaagtat
     3541 cagaagctga cgtaaaagaa atgatgcggg aaatccgcct tgccttacta gaagccgatg
     3601 ttaacttgca agtagtcaaa gattttacta agcgcgtacg cgaacgtgcg gtaggggcag
     3661 aagtgctgga aagtttatca cctgcacaac aaatcgtaaa aatcgttgat gaagaattga
     3721 cgatcacact tggttctgaa acagcagagt taaataaatc accaaaaata ccaactgtta
     3781 tcatgatggc tggtcttcaa ggggcaggta aaacgacctt tactggtaaa ttagccaatt
     3841 atttaaagaa aaatgaaaat gctcgtccgc tcctaatcgc aggggacgta tatcgaccag
     3901 ctgcgatcga ccaattgaaa gtattaggtc aacagctaga cgttcctgta tttgatatgg
     3961 gaactgaagt cagcccggta gagatcgtcc gtcaagggat ggagttagca aaagaaaaga
     4021 aaaacgacta tgtattgatc gatacggcag gacgtctgca tatcgatgaa acattgatgg
     4081 atgagttgaa acaaatcaaa gaattgacgc aaccaaatga gattttgctt gttgtcgatg
     4141 ccatgactgg tcaagatgcc gtcaacgtag ctgatagctt caaccagcaa ttaggtatta
     4201 caggggtcgt catcactaaa cttgatgggg atactcgagg cggggcagca ctatcgatcc
     4261 gctcagtgac aggcgcacca atcaaattca tcggttctgg tgaaaaattg actgacttag
     4321 aagtattcca cccagatcgt atggctagcc gtatccttgg tatgggggat atgctgacat
     4381 tgattgaaaa agcgcagcaa gactacgacg agaaaaaagc ggaagaactt gcacagaaaa
     4441 tgcgggaaaa ttcattcgat ttcaatgact ttattgaaca gcttgatcaa gtgatgggta
     4501 tgggcccact ggaagatcta atcaaaatga tcccaggaat gaatcaagtc cctggaatcg
     4561 aaaacgtcaa ggtagatcca aaagatgttg aacgcaaaaa agcgatggtt tattctatga
     4621 cgccagcaga aagagcaaat cctgatctgt tgaacccaag ccgtcgtcgc cgaattgctg
     4681 ccggttctgg aaacagtgtt gttgaagtga atcgaatgat caaacaattc aaggaatcaa
     4741 gaaaaatgat gcagcaaatg tccaaagggg atatgaacat cccaggtatg gaccaaatgt
     4801 ttggttcagg cgtcaaagga aaactaggca aaatggcgat gaatcgaatg atcaagaaaa
     4861 ataagaagaa aaagaaaaaa cggaaataag atgaaaaaaa cagcttcggt acagactgaa
     4921 aatgtctact gccggagctt ttctatcagc aatttttatt ctttactaat acaattactc
     4981 taaacgaaga ggtggcacag atgagatgtc cgtggagtga acaaacagaa agtatgcggc
     5041 aatatcatga tactgtctgg ggtgttcctg aatatgatga ccaaaagctg ttccgaaaat
     5101 tgatgcttga tattaatcaa gccggattga gctggcagac gattttaaat aaaagtgaag
     5161 cattcgatga agcatatgat cgattcgata tcaaaaaagt cgcaaattat ggagaaacaa
     5221 aaatcagtga actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa atcgaagcag
     5281 ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt ttttcagagt
     5341 atctttggtc gttttctaat ggaaaaatcg ttgattcttc ttatcattca caagaagaag
     5401 tcccaacaac aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa agaggattta
     5461 aatttatcgg aagcacaaca atctatgctt ttttagaagc tgtcgggatc atcaatgatc
     5521 accttgacag ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag ggcgaataaa
     5581 gcatgcgaaa aatcatattt tatggtgcta taagtttaga tggctatttg gcaacgaaaa
     5641 cggatgatct tcaatggctt tttgacactc caacaggtaa gcaaactact tatgaagctt
     5701 tttatgaaac aatcgatacg accatcatgg gcagaaaaac ttatcaggaa gcaaaaaaat
     5761 atttggagac cgaagcaatt tatccagaga aaaagaattt cgttttttcc acccacgcac
     5821 aattacaatt gccagatgct gaggtcgtaa acgaagatcc tgttgctttt ctaaaaaatc
     5881 tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg atcaaaccgc
     5941 tattggaaca tcaaatgatt gatgaatggt atatccagat tacccctgta ttattagggg
     6001 acgggattcc tttattccaa aaaggaacat atgaagcgcg ttttgaaatg ctagacacga
     6061 cacgattcgg tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa ataaaataaa
     6121 aaaggcaact atatgcagaa atgtcctgta catagttgcc ttttttatta ccgttatgct
     6181 ttttactctg ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt ttggaagaaa
     6241 cttaatgttc cttcaacgat attaccattt tcatcaatta gattcgcaac gtttccgata
     6301 tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg gcgtagatgg
     6361 tggtttgcgc caaagccgcc gatcccacct ggtgatgcag taacgactaa acctggttta
     6421 tgatcccaaa cactgtgtcc ataaggtctt gagcctacat ctaaagcatt tttcaatact
     6481 gctggcacag aacggttgta ttcaggagta acgaaaacga caccgtctag tcccctcact
     6541 tcctcacgaa aacgagtcca ttcagcagga gcctgttctg gagtttcgag atcctcattg
     6601 taaaatggca gatcatcgat ttttacaaat acactttcgt acccttcagg aagcaatttc
     6661 gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc ttaaactacc aataaaaaat
     6721 ccaatttttt ttgtcatacg atttttcctc cttcaaacta tctataataa tcttacaaaa
     6781 gataagtaac tggagcaaat aaaaacactt actgacaaaa gctacataga agatagatta
     6841 cttattttta acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa tcgaatttaa
     6901 tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac tacatcttgt
     6961 gaataaaaca atggaggtgg aaataaataa tggcagttaa aatccgttta aaacgtatgg
     7021 gttctaaaaa gagtccgttt taccgtatcg tcgtagctga ttcacgttct cctcgtgatg
     7081 gacgtttcat cgaaactgta ggtacttaca atcctttgaa agaccctgca gaagtagttt
     7141 taaaagaaga cttagttctt gactggttat caaaaggtgc acaaccttct gacactgtac
     7201 gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa ttctcaaaga
     7261 aataaggtga ccggatatga aagatttaag cgacttagtc ttaacaatcg ttcgtccgct
     7321 agtcacttat cctgatcaag tcaagttaga cgtcgtagag tcgaaagact tttacgagta
     7381 caatctaaca gtggctcctg aagatattgg ccgtatcatt ggaaaacaag ggcgtgttgc
     7441 taaagcaatc cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa aagtgcgttt
     7501 gaatatcatc gatgataagg aaaagtaaat aaacgtgctg tactcgaatt ttcgagtgca
     7561 gcttttttgt tttttttatt aattgaagaa atgattattt ggcaaaacaa gcaagaaagc
     7621 gtatccttat aggaggaggg agtgaacagc caatgagaaa gaaaatgtta tttattccat
     7681 ttgctctatt gttaagcgct tgtagttcgg ttactcaaga atcagacaat acacatctat
     7741 caagcagtat atctgaaact gttaccagtc cagaaaaaag tagtaccaaa acggcttcca
     7801 ccactgaaca aacaatggct acctcaaata atgagaaaaa gactgcttta gaccagttga
     7861 aagaacaaca gccaaatgtt cccatgcctc tagatgtacc agtctcttca ggctatctca
     7921 atatcgcagc gacacataca aagcaaggtt attctatttt atattacaga acagatcgtc
     7981 ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc tattgcaacc tatctatatc
     8041 aatatgggtt tgcttctagt caagaaacaa tccaagtttt gcagccattt gagatcgaca
     8101 caaatggtca gcaagtggac ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag
     8161 gttcaagttt tcttgaatgg caagaaggaa actggcgtat tcgaatccgt gggaataata
     8221 tagaaggcca agatccctta ctattagcta aagaaatcgt agcttatttg gaagaaaaca
     8281 gtctaccagc acctgaacaa tttggtaaaa ttactgttga tatgggtgat acgacgaatc
     8341 gagccgtaga agtaagctgg caagaaccga aaaatgccta tacgatcact catcaagatc
     8401 ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc
     8461 tgagcccatc atttaactcc aagtcacagg aagtaatcaa aaatagcacg ccgcattttt
     8521 gcataatttt ggcttaatac cttaaaagaa aactagaatt gcctcttcac acatgctaaa
     8581 ataaaacaga caaaaaagaa atggggatat aagttgactg aatacttgaa tgtaggaaaa
     8641 atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac ggatttccca
     8701 gaagaacgtt acaaaaaagg aacggtgcta acactattcc aagacggaaa agcacctgtg
     8761 gaattaacag tcaaaagtca tcgcaaacat aaaaattttg acttattgag ttttgaagga
     8821 catccttcta tcaatgatgt ggaaaaatat cgggatggca tattaaaagt gtcaaaagac
     8881 aactctgtag atctagctga aaatgaattc tattaccatg agatcatcgg tctgcatgta
     8941 ttagaagatg gaactgagtt aggtaaagta aaagaaatct tgtcaccagg tgccaatgat
     9001 gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc ttccatatat cgcttctgtc
     9061 gtcaaagaag tcgatttaga agctggagtg gtccgtgtag aaattccaga aggactgatt
     9121 gacgatgaag attgatgtat tgactttatt tccaagaatg tttgaaggtc ctatggggga
     9181 atccatcatc ggtaaggctg tagataaagg attattagat atcaatatct ctaacttccg
     9241 cgattattca gataacaaac atcagacagt agatgattat ccatacggcg gtggagcagg
     9301 tatgctgctc aaagtccagc ccatctatga taacatccaa gccattgaaa aagctgcacc
     9361 ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt aaacgattcg atcagaaaat
     9421 ggcagaagaa ttttcacaag aagaacactt agtattcatt tgtggtcatt atgagggata
     9481 tgatgaacgt atccgcacat tagtgacaga tgaagtttcc ttaggagatt atgtccttac
     9541 tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt tgccagatgt
     9601 gttagggaat cagacttcag ctcaaacgga ttcttattca accggtttat tggaacatcc
     9661 ccaatatact cgcccggcag aatataaagg gatgaaagta ccagaagtat tgacaaatgg
     9721 aaatcataaa ttgattgaag aatggcaatt aaaagaatca ctaagaagga cctatcaacg
     9781 tcggcctgat cttttggaaa cacttgaatg gacacctcaa atgacgaaat ttttagaaga
     9841 aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc aagtgacttt ttctaaaatc
     9901 ttctttacag taggttgata atatgttagt attttatggt gagtgcaaaa gcacttaact
     9961 attacgatat tccgctgtga gaaaagctca taagaatatt tggaataagg agaagaaaac
    10021 catgaatcca ttaatcgaag aattaacaaa agaacaatta cgttctgaca tcccagcgtt
    10081 ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat
    10141 ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac
    10201 agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc atacaccacg
    10261 tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct
    10321 acgtgcatta cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat catcgttatc
    10381 agactgagaa agaacccttg gtattcaagg gttctttttt tgttctctgt ttttttatat
    10441 taacgatttc tatttgggac atttttgatt aataaaaagg ggcaaaaaag gggcaacaag
    10501 ttacagtttt tctatatctt ctttcaattt ttcttttact ccctttgtca catgcaaata
    10561 aatcttttca gtgatatcac tattttcgtg cccaacgcga tcctgaatag catacagagg
    10621 agttcctagt tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg aactaagttt
    10681 tttatcaatc ttcatgtcag ctttatgatt tctcaaatag gtgttaattg ctgttagttg
    10741 gaaaggagtt cctttcgttg tttgaaatag aaattgacca ttcggattta attctagaag
    10801 ctcattgtaa atagctatcg ctttttttgg taaatcaatt tctcgcattc cagcagcagt
    10861 ttttgtagaa tcagattttt tcatatcagc tattgaacgt tctctataca tcatcgtccc
    10921 atttataact actgatgcat tattattggt aatgtgtaca tcgtctttac ttaatgcgat
    10981 tgcttctcca ggcctcattc cagtcaaata tagccactgg aaaaggagag aatatctttt
    11041 attgtgtgaa gttgtgaaat ttactagtct tttatattca tcatcttcta aaaacttatc
    11101 tttaattttt atcgtttttg actctcgttt ataatcaata actacttcgt tgataggatt
    11161 tttttctaca tagccttttt tcatagcgta tgaaaaaaga agattcaatt tggatttaat
    11221 tacactgacg tacttatttg acaagtcatc tttatatatc atatcttcaa aaatattatt
    11281 taaatctact gtattaatac cagaaacgat gtaagtctca ggtatctttt tctttatagt
    11341 gtttaagata ttattcgtag gataatatgt agactcctta acttgtcttt tgtaaataac
    11401 taaccattct tcgacaagct catgaaatgt tagatcaggc ttctgaagtg ttttttcgtt
    11461 taattttata tcaatttttt tatttaactc tagcaatgcc actttctgag tttctcgaga
    11521 tttattttta taagtaatgc tcactttttt tctttttcta gtttttggat caatatatcg
    11581 ctcattatat ttatatactt tttttccgtt tttatcagtt ttggtttcaa tccacattgt
    11641 tttcatctcc tatttgttgc tataatagga atggataagt aagccaatta tagcaggttt
    11701 atttttcatc acgtccacaa actttggcga gggagggggc gtgttttttt gtttagtagc
    11761 tacctttcca tgaagtatct gcgaagaaca cttcaaatgt tgttccatca tttttcactt
    11821 caaaatatgc tgttccttgt gcggatttac ccggttggat ggtttccgaa aagtaatctc
    11881 tactagctaa atcagattta atatctgatg aatcataaaa ttctaaataa cttgaacttg
    11941 catcaaaagg tgtagtccct gtgttttcta caagaaaatc tacctttgcg taaaataggc
    12001 cttctggttt atagaaatca tcaccgtaat cttttgatac agactggaca gttacgttaa
    12061 tagagttacc attttcagaa gtaaaaggaa ctgcttctcc tatactatgt gcaactgatg
    12121 ccgtctgggg ttctgaagac gaagttgtag ttgtagttgt agtagagctt gaatctttgg
    12181 attttccatc tttatctaag aaagttcgtt gattataata tacaacctgt tcagtttttg
    12241 gactaaccca aattaacatt gtttcatcat cagaatcagt tgaataagta tattggagca
    12301 tctctaattt ttttatatct tttgcactat ttccatgttc aattaattca gagtgctctt
    12361 tataataatc tccttgtgca tcctttgaaa tagatacaat taattcatcc cgtgtgatag
    12421 cattgtctaa ttctttgtaa acaaaatcat ttgtccattt acgtaacggt gaaccaagtg
    12481 tctgataaac ttgagttaaa tcatcaccaa cttttatttt atgaacgtct gatgaagtta
    12541 gtttttttgt ttcatattca gatgttgatg aaacagtatt ttctttagtg ctagcagaat
    12601 taggactagt ggagcatccg cttaatacca agcctaaaaa aagcaaccca aaaacaattt
    12661 ttttcatttt taattcctca cttctatgat atgatttttt tgtaggatct tagaaataag
    12721 gtttttagtc cgtgttgcag cacggacttt ttttacatta tataagaaag cgataagcgc
    12781 tttctggaag tccgtaaaga ttctttaatt cctcaattcg tttaggatat tgatcattgt
    12841 cttctttgta tagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt
    12901 tgcttctaga tgttcttgtt gatacatagt aactggataa gccacgatga aaaatagcgt
    12961 gacctaattc gtgagcgcaa atgtagaatc tttcctcaga gtctctcagt tcatcattta
    13021 agaagattat tgcacgacct ctaatttctt gaaactgtcc tttgggattc tcgataaaag
    13081 gaacgtattg aattttaatg cccatctttt cacaaatata aaaaggatta gcggactggt
    13141 atttccgctt caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt
    13201 ttgccttttt ctttgtcttc tttcacaata tcccagaagg ttccaataag gatatctttt
    13261 acgcgttgga cctgttcagg cgtcagtgtt tctccgccat aagacatatt gacatttgaa
    13321 tctagtaatt tatcaagttc aactacttca tcttttgttg cccagtcagg aacttggttt
    13381 ctaccgagta aataatcagt agttacatta aaataatctg ctacaagttg tagtctttta
    13441 gtactaggag ttctattttt ccattgataa atcgtgtttt taggtatatt taattcttct
    13501 tccaattgag ttatacttat gcctctttta tgagctaatt cctttattct atctagtaaa
    13561 ttcattttcg cacctcacaa gctacgaaaa cactaataaa aaaattagtt aaaggtgttg
    13621 acaactaata aaagtgttag tatactgttt tcgtaagcta aaatattagc taaaaagact
    13681 tgataactaa taaacacttc acggtcggca aactctgaaa tgtaaattac taggcatttc
    13741 tgtgtcttat ttagctatgt ctatatacta ataaaaatat tagttattgt caacgaatat
    13801 tagctaattt tttagcttac agattatttt tttagaaagg agctattttt atgtctgaga
    13861 atttagactt aaaaattcga gcggagatga gaaaaagaag aatgactttc aaagaactag
    13921 ctgcgcttat cggtatttca ggagcttatt tatcagatat tctaaacggc aatcgtgatg
    13981 gaaagaaagc acaacagcat atcgaaacag tgaaaaaaat attggacatc cgataggggg
    14041 gtaggcgatg acaaaactaa aaaaacaaga ttttgtaaaa aaatacaatt attctccatc
    14101 tacttatcaa cgtcgaatgt cggaactaaa aaatacagca attttctcag cggcgtatga
    14161 acgggtcaca ggacaagaag tttggatcaa tacagaatta tacgataaat ttttgtcttt
    14221 caaatcctat aacaggttac gcacaagaaa ggtaacacct aaagaattta tcgagaagca
    14281 tttagttgat ttataaaaaa cagaacattt tgagaggtga aggttaatgg gtaaattcaa
    14341 cagagcattg gtattcagcg caccgctaat catctacgct ttaggacttt ggggaagcag
    14401 gcaagcgttg ataggaacga ttgtttacat ggtttggatt tttatggggc ttgatgaagc
    14461 tgaagctgag tacagagcga aaaagccaac cgagggggct gactaaaaat gaatagaaaa
    14521 gagaaactag aatgtatatt actattactc agtttaattc tttcactaat ttctctattg
    14581 ggaagtttgt atttttgata tcaacaattt tgtggatttg ggttctattt agagtaaata
    14641 tcatttttgt ttggtgatta aggagtttat taaaattaat tttccctact tcaaatgcaa
    14701 ggtaacaaga tatagcactt ttaggaggaa tatttagcgg tacagcttca gagtatattc
    14761 tattatcatc gactgatcct cctttagcaa ttaatttctt aaatggagta gctgaccatc
    14821 tgcggtttaa gttagcggat gaaagttcta aattaactaa catagctggt tctgttgaat
    14881 agttagaaat gattactttt gtataaatca tatctgcagc gaagtaagaa gcattaagtt
    14941 caacttctag ttgagggcgc attcctttta tttttatccc tgttaaaaac gtactcaata
    15001 taaaacttat tattgacatc cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca
    15061 ccaccagttt ttaactaaat tataccaaaa aggagagaag aaataatgca agaattagta
    15121 attttgaaaa ataaagaagc tgtgactacg agcttacaag tggcagaaag cttcgagaaa
    15181 aaacatcaac atgttttaag agatattgat gcactaaaaa aagatgtgtc caattttgga
    15241 cagatgtttg tagaaggtaa tgaaccagat tcatatggca gaaatcgacg agttttcttc
    15301 attagtagag atggattttt cttgctggct atggggttta caggaaagaa agctatctac
    15361 ttcaaacaaa aatacattga agcattcaac gaaatggaag atgttattcg caagaatact
    15421 gttcctcaaa caattgaaga tatgatgatc tatcaattag aagaaatgaa agatgttaaa
    15481 aaagatgttt ccatgcttaa agatactatg cgaattagcg gacaacaaga gtttgaaatt
    15541 aagcaaaaag gaaatatgaa agttatggga gttctagggg gaaaagaaag ccgagcttat
    15601 gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg taccttttca
    15661 atcccaagat atggcgagtt acctcgtaag agattcgatg atgctgtttc atttattgaa
    15721 atgtggttgc cagaaactgc gatccgtatg gaaattgatc aactgaacag acaacagaga
    15781 ctttttggtg atgacaatga atagagctga agcgctaaga atagggacgg caattgctaa
    15841 ttgctggtgg aaatactata aaccaatcat cctaagccaa caacatattg acaagcaaaa
    15901 agcatggcaa caaataaaaa agtgactccg ccggcaagca aagagtcaca aagaaaacac
    15961 atcataagga gattttagca tatggaaaaa gaactttcca ctctagatca atatttgatt
    16021 gatcctgatt ggggcaagcc gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat
    16081 cttttgacga atgaagaact agcttgggat caagatgatt taggcaacca tgtaactatt
    16141 tgggatcatg tttatcttat ccatctatcg aagcattcga ataaacctga atatatttac
    16201 gtcatcgaag atggcttgat tgatgcgcta gaagagtacg acagagataa cttgattgat
    16261 atctcttatt acggaccagg taagaaatac attgctgaaa tggaggcaga atttgatgag
    16321 tgaaatcaaa gggacaacga actttgaaaa actttttagt cgtaagttaa ataaaattct
    16381 caagaaaaaa ggaaattttg attatttatc ttgggctcac gcgtgggaga ttatgaaaaa
    16441 gaatgatcca caggcaacgg taactattaa tgagtacaaa cactacaggg ttgtttctgg
    16501 aactcatcaa gactttcttg ttgaggaata taaacctttt cttatggatg aaactgggac
    16561 ttatgtatct gtctcagtaa cggttaaagg acacacggaa accga
//
LOCUS       DACECJ010000064        16590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.064, whole genome shotgun sequence.
ACCESSION   DACECJ010000064 DACECJ010000000
VERSION     DACECJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.064"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..786)
                     /locus_tag="IWO09_002073"
     CDS             complement(79..786)
                     /locus_tag="IWO09_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0264658.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(865..1203)
                     /locus_tag="IWO09_002074"
     CDS             complement(865..1203)
                     /locus_tag="IWO09_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0264659.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1314..2276)
                     /locus_tag="IWO09_002075"
     CDS             complement(1314..2276)
                     /locus_tag="IWO09_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0264660.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2291..3622)
                     /locus_tag="IWO09_002076"
     CDS             complement(2291..3622)
                     /locus_tag="IWO09_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0264661.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3630..4310)
                     /locus_tag="IWO09_002077"
     CDS             complement(3630..4310)
                     /locus_tag="IWO09_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0264662.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4529..5008)
                     /locus_tag="IWO09_002078"
     CDS             complement(4529..5008)
                     /locus_tag="IWO09_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0264663.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5135..6814)
                     /locus_tag="IWO09_002079"
     CDS             complement(5135..6814)
                     /locus_tag="IWO09_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0264664.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7362..8864
                     /locus_tag="IWO09_002080"
     CDS             7362..8864
                     /locus_tag="IWO09_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0264665.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8877..9539
                     /locus_tag="IWO09_002081"
     CDS             8877..9539
                     /locus_tag="IWO09_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264666.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9640..9843)
                     /locus_tag="IWO09_002082"
     CDS             complement(9640..9843)
                     /locus_tag="IWO09_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0264667.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWO09_002083"
     CDS             complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWO09_002083"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0264668.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWO09_002084"
     CDS             complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWO09_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0264669.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13807..14421)
                     /locus_tag="IWO09_002085"
     CDS             complement(13807..14421)
                     /locus_tag="IWO09_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0264670.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15027..15404)
                     /locus_tag="IWO09_002086"
     CDS             complement(15027..15404)
                     /locus_tag="IWO09_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264671.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWO09_002087"
     CDS             complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWO09_002087"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0264672.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4807 a         3574 c         2833 g         5376 t
ORIGIN      
        1 ttccaaataa atggtattca atcagctaac tctcggtatt agctcagatc aaagcgctga
       61 tttcacaact tctttatttt aatctcttcg gttgccgccg aacaataaga tcaagcgcaa
      121 cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc agtgctgctg caaataatac
      181 gtgtcttgcc atcggtactt cttcttccgt cagtagtccg ccttcactca aaatttggat
      241 tgctcgccga gacgcgttaa attcgactgg taatgtcacc aattggaaaa gtaatgctaa
      301 agaaaaagcg aaaatcccga tattgattaa cgtagagttc cagctaagca atacacctac
      361 cataatcaag gggaacgaaa tcatcgaacc aaagttagct gctggaacaa gtgctgcacg
      421 aatcttcaat gggacataac ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc
      481 tacacctatc gcagcaactg aagtagactg tgcagttgct tgagacaaac tcaacatctt
      541 attacctgaa ttgtagttat ctgtgagatc tccggcaatt tgttgaacac cgacattatt
      601 tatctgttga ctttgtaata tatattgtgc tgcttgtgta ccagtcacat tgtttttgct
      661 tctcacttga tcgtattttc ggaaagtact attgacgtac gctgacgcaa ttccggaaat
      721 aactaatcca agaatgacca aaagaaatgt cggatctaaa aaaccataca ttccataacc
      781 atacattttc gtttcactcc ttttcgttta ctttatttta tcattagtag taggaaaaga
      841 atgtgaaaag accttgaata ttctttaatt ctttttcaca aattccgatt tcagtttcat
      901 cggtccaaat ccttctacct tgcaatcgat attatgatcg ccttctacta aacggatgtt
      961 cttgactttt gttccttgtt taatggcacc tgacgcacct tttactttca agtctttaat
     1021 cactgtcacg ctatcgccct ctaacagacg gtttccattt gcatctaata ccactaatcc
     1081 gtcttctatg acagtttctt gttcttcttt gttccattcg ttcccgcatt ctgggcaaat
     1141 caacaagcct cgatcttcat atgtgtactc tgagccacat tctgggcaat ttggtaatgt
     1201 catcttcttt ctcctttaaa atctttagtg agcactattg taacggaaaa agaagatctt
     1261 gaaaagatac atttgtgatt tttactacaa aaggcacgtt ttcgaacgaa gtatcattct
     1321 gtttttctgc tcttatacga ttggcatata tcttggatct ctttatagat ttcctctact
     1381 tctgctaaat cttttgcatt cgcaccactt gctaatggat ggcctcctcc atgatggcgt
     1441 ttagccaatt cattgatgat cggcccctta gaacgtaaac gtacgcggta ataccctgaa
     1501 ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa tcactccagg aagtgaaacg
     1561 atcgaagctg tctctgaatc atcgattcca tatttatcaa gaatttcttg cggaaggatt
     1621 attcttcctg caccattttc atccacctgg atattttgat aaagataacc ggaaagttta
     1681 gcgactttca tcggcatttg ttcaatctct ctgttcaaag cacttgcatc aaatggataa
     1741 tttctcaata tcgaagcaat ctctaatgta cgggacgtcg tggagggata caaaaagcgc
     1801 cctgtatctc cgacgattcc tgcataaagt aaccgtgcag ccttaacgtt catctttaaa
     1861 gattcaggaa acatcaaagc aaattctgca atcatctcac tacagctact tgcttttgta
     1921 tttacccaca caagatctcc ataagggtca tcattgggat gatggtcgat tttgatcagt
     1981 ttttctgcca actgatatct ttgatcactg attctgggtg aattagctgt atcagtcacg
     2041 atgactagcg ctccttggta gaagacatct tcaacttctt gcatttctgc taaaaagcga
     2101 agcccttcaa tcgtttcacc tacttgacgt atttcttttt ctgggaaact ttcccgtaat
     2161 agttcggcta atcctacttg tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg
     2221 ataatgattt tatcgtattt ttttattgta tctactattt cttgctgtac tgtcatttca
     2281 ctcctgcttt ctaagaacgt tccattactt gacaaacaac gattgctttg gaaacgatgg
     2341 tgttttcaat aaacacttcg atatccagtt tagcggaacg tctgccgatt tccaatacac
     2401 gtggacgaat gtctaattcg ctttccaatt ggatcagccg caaataatgt aggttcactt
     2461 gttcaatcaa gatatttcgt ttttggttca tcaccattgt tttttgtgcg acatcagaaa
     2521 tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta
     2581 ctacgaattt gaacttaggg ttttccaaac gatttccttc acggtcagta tcaatcgtat
     2641 ggatatttcc agatatctga tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa
     2701 gctgcatcgc tttcattaca tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg
     2761 aaacgaccgg catgacttct agcccatccc agatcatttg atggctaaca gaggcaatac
     2821 tcattgtttt ctttacactg attggttctt tcgtcatcac ccgatcgatg atctggtttg
     2881 gcgatttccc taaaacatct tttgccgtaa caatccccat caaacgatga tggcggttga
     2941 cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat
     3001 ctgtctgctg caaataatga gtcttttcag ctggcatata gatatcactg acaagcatga
     3061 tatctttttt gatcaattgg tcgcttaacg ctcggttgat catggttgcg actgtaaatg
     3121 tatcataaga ggtgcgtaag atcggcattt caagatcatc agctagcttt gcaatctcag
     3181 cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc atcttccaat gctaattttt
     3241 ggacttccgc ccggttgccg acgatcatta gtgatcctgg cgtaatatag cgttccatgg
     3301 catgtttagt catcgcgccg atgacgaatt tattcaagac cttgtctaat cctgccgccc
     3361 cgcctaaaac gtccccttca atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat
     3421 gtttttttaa tttgcgttcg atacggatgg tccctactcg ttgaattgtt gaaactagtc
     3481 cgacattttc tgcatctttg attgcacggt acgctgttcc ttcactgaca ttcaattctt
     3541 ttgcgatgct tcttacagaa attcgattgc caacaggtaa actttcaata tattctaaaa
     3601 tcaaatcatg ttttgtcgcc attttctgcc tacacttcca gcgtttccga gacgctcatt
     3661 atttttcctt cactatcttc cagtaactct acaaaagatt cgggatcttg ttcaatgaca
     3721 gggaaagtat tgtaatggat tggaactact ttcttggcac gaattgtttg cgcagccttt
     3781 gctgcatctt ctggccccat cgtatagtta tcgccaatcg gcaagaaagc gacatcaata
     3841 tcgaattctt ctgcaagcaa tcctagatca ctgaaataag cagtatcccc tgcatgataa
     3901 atcgtttttc cttctgcttg aataataatt ccgctaggct cgcccatata ctgaacgata
     3961 ccgtctactt catagcttga actatgctgt gcatgtacga attttaccac gccgaatgga
     4021 aacgcatgtt ttccaccgat attcataccg aatgaatcta ctcctctgct gttcgcataa
     4081 tcagcaatct caacaatact gataattgtg gcttgatttt cttttgcaat aggaatcata
     4141 tctcctatgt ggtcactatg accatgagta atcagcaacc aatctgtctt tacttcttct
     4201 gcagttaagt ctgtcaatgg atttcctgta ataaacgggt caatcatcac gcgttggcca
     4261 ttttccattt caattgttaa acaggaatgt ccatgatacg taattttcat attttacacc
     4321 tcgcgttttc tctgtatctt ttttattaaa aacgatttca tcacttgcac atcaatgaaa
     4381 aaatatagct gctaaatata ttttcattgg attgatagta cagtatgtac aaagcaattg
     4441 taacagataa tacaggatgc accaagtacc aagcagatat aatctcaagt ttgaggtctc
     4501 tgctctgctt agcttctctt ctcgtccctt aactaattaa atcagcgtaa ataggatgga
     4561 cgatcttttc taaatcggct acttttatcc gaaggcttct cccgatttca ccgcttgaca
     4621 caattacttc ttctttttct tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg
     4681 cgagttttat tccaataggg gtgtttgctc cgtggggata tcctgtatgc attaatacaa
     4741 aatccattcc aggaaaacca actttacgat ctttcgtcac ttgtctcgtt tttttgtaat
     4801 ctaagtgatc gttcaaagga atcaatgcga tgatcacccc agttttgttt cctttcacta
     4861 cgagagtttt acatatcaaa gctggatcaa tccctctttt ttgtagttct tctgtgaaag
     4921 attcttctac cacatgacta aaatcaagtg gttgatagct gatcttttgt tcttctaaat
     4981 aacgttcgac ttttgtttga ctcatcattg attcactccc tttattctat cgtttattat
     5041 aaagcgaggg atgctgtatt caaaaattat ttgtttttcg caaaaaaaga gcaagacgag
     5101 cactctatag agaggttcat cttactcttt ttgtttattt tttatttcga cgttgagtag
     5161 cttccataat gactggcaag atcactgggc ggcgttttgt ttgttcaaat aagtaacggc
     5221 ctaattgatc tcggatgtct tgctttaatt tgctccactc aaaatcattg ctttctaaat
     5281 gtttctcaac gattgtttca atcatattgc tgctttcttt catcagatca cggctagctt
     5341 ttacgtaaac gaatccccgt gaagtaattt gaggttttga aatgattttc ttttctcgcc
     5401 gactgatagt gacaaccgct acgaaaatcc cgtcttctga caagattttt cgatcacgta
     5461 aaacaatgtt tccgatatca cctatgccta atccatcaat cattacattt tctgcacttg
     5521 tagttcctgc aacactcata cgcttgttct tatattccag cacgtctccg cgtccagtaa
     5581 tatagatatt tttgtatggc atccctactg catgagccaa atctgcatga gcagctaatt
     5641 cacgatactc tccttgaact ggcacaaaat atgttggttt catcaaattg atcattaatt
     5701 gcaagtcatt aggattggca tggccagaaa cgcgcatatt ctcagagatc aacttcactg
     5761 ttccaccagc tcgataaatg atatcttccg ttttcgccac gatggtttcc atcgcgatgc
     5821 ttggcgtagt agtgatataa accaaatctc cttcttcaat cttgacatta cggtgtgtac
     5881 cgtttgccat tttttgcaga gacttgatgg gttcacccat tcggccagtt tctaaaatga
     5941 tcagttcttc tggatcatag tttttcatgt ctttcaacgt aacaagcaga tcttcacttg
     6001 gtaattgaag tttttctaaa cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag
     6061 tcaagacaac ttttcgttgt gctttttctg ctgcatccaa tacttgttgc actcgttgca
     6121 aattacttgc tacacaagcg acgatgatac gcccttccca atatttgatc gtatcaaata
     6181 cttcatctgc tatttgggct tcagaagcta caggctgagg attttctgca tttgccgatt
     6241 cacttagtaa ggctaagact ccttctttgc cgatttccgc caatcgtgca taatctgttt
     6301 gatacattgg gatagccgtt tggtcaaatt taaaatctcc cgtataaacg atactacctt
     6361 cttttgtttt cagattgatc cccactgaat ctggtatcgt atgagtggta cggaaaaagc
     6421 tcactactgt ttcaccaaag tctatttctg tatgctcatc taccacatgg aaatccttaa
     6481 atcctttggc tgcttcatta cgactgacat tcaattttgc taactcgatc gtcaactctg
     6541 tcccgaatac tggtacagaa atttttgaca acaagtaagg aagtgccccg atcgcatctg
     6601 catgtccatg tgttaaaaag acaccagcta cacgatcaat gttttcttct aaatacgtaa
     6661 aatcagggat tacgacgtcg atccccaaca actcattttc aggatatttt agcccacaat
     6721 ctaaaataaa aatatcctcg cccacttctg cgacatacat gttttttcca ttttcacgaa
     6781 cgccgcctaa tgggataatt ttaatggtac tcacaaactt cacctcttct tatgacatcc
     6841 aagtttaaag atgtcgattt tcttctttat ctaaatatat gagccattcc aatgaagcat
     6901 atgcaaaatc actatttctc aacttatgga gaatcagcag gaaaattacc agcacatccg
     6961 cgttctgaca gttggatggg cattttattt tggatatact tcattaacaa aatggatctt
     7021 ttgcgaaacg ctatagccag aatacttata taagtattca gctgttcccc attattatac
     7081 aacaaataaa ctaaatcgct agtatcagca attgttagaa caactatgca cgtaaaaatc
     7141 cctctgtagc atctaggatt tcttttgcta ttcctctttc atttcttctt cgtcggtttg
     7201 aaacatactt ttttcatttt accacatttt ttgcaataac tatagtgact ttttatatat
     7261 acagaacctc atgttgatgg tgtgcaaaca gcataagatt taagctagct ttaagatagg
     7321 ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg
     7381 catggaaaag tacaaaagta aaatggggac gttcattgtt attgtttgcc gtgttcacag
     7441 ccattcttat ttttgtgcta gctggcctca cgattcgaag cgctgcagaa gaagcagcga
     7501 atcaagcaca aaaagaagtt ggagcaacag tgactttaag tgccaatcga gaagcttcat
     7561 ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac tgaagaacgt ccagatccag
     7621 gcagtttttc tcttacacca gtttccttaa cggacgcgga aaaaatagct gaattagata
     7681 atgtaaagag ttattccttt gaaagttccg cctcagctat ggcaaaagat ggaataactg
     7741 ccatcactag ttctgattca agtgaagaca gttcagaaac tactgatgca ggcggtgaac
     7801 ctgctggagg aatgggcggg aatccgcaaa tgactcaagc agactttcaa atcagtggtg
     7861 tgtcagcaac tgcacaaaat agtaattttt ctgatggaac agcaaaaatc acggatggtg
     7921 aaggaataga tgacagcgac aaaggcacaa acaatgtagt cattgattct actctagcag
     7981 aaacaaatga cctggcagtc ggagatactt tcacaatcac gagtactgaa gatgaagata
     8041 ccacttatga aatgacgatc aaaggaattt atgaatccag cgaaacaagc ggcagtatgg
     8101 gtatgaaatt caacttcatg aatccgtcga atacacttta cacttcttac acttttgcaa
     8161 atgaactaaa tgggacatct gacgataaca cgatagattc tgctatttac actctatctg
     8221 atccagataa aatggatgag tttctcaaag aagctgagaa gctgatcgac acagatacat
     8281 ttagcttaca atcaaatgat tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg
     8341 caagtttttc taaaaatgtt gttttactag tggctgtagc cggtattatc atacttactc
     8401 tgatcattat gattaccatt cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag
     8461 gagaatccag aagtaaaatc attctccaat tgtttaccga agtggctatt tgtatgattc
     8521 ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc
     8581 tacttgacca acagacagaa accacgacac aaaaccagca agctcctggc ggtggacaaa
     8641 tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg tcaacaagga accacgaata
     8701 atccatttgc tgtttctgat caagtcagcg aattggaaat caatgtcaaa cctgcacaat
     8761 tgggactatt agccggagta gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta
     8821 tagggatatt aagattaaac ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt
     8881 tacggacaga acaattggga ttttggtatc aagatccgca agatgcgctt tataaagatg
     8941 tagaactgaa tttcgaagct ggaaaaatgt acgctatttt aggagcaagt ggttctggaa
     9001 aaacgacttt tctttcgctt atttctggac tagatgcacc aaaagaagga aaggtatact
     9061 atcaagatcg ttcactgaac aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga
     9121 ttattttcca agcttacaac ttgcttcctt atttatcggc tctagacaac gtcatagcag
     9181 caatggaaat cactaagacc aagcaagaga attatcgaca agttgcttta gatcgactgg
     9241 ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt gaccaaactt tcgggaggtc
     9301 agcagcagcg agtcgctatc gttcgtgcta tctgctgtga acatcaacta atcgtagcgg
     9361 atgagcctac tggaaatcta gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag
     9421 aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa
     9481 aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa
     9541 aaacaggata atgatcaaat aaaaaatccg aaccatatct gtctcgcaga tatggttcgg
     9601 attttttatt tgcttgattc aattagtaat aagtggtttt tatttttttt cgtcttttcg
     9661 agaaaacttg tctttgactt cgtcgcctaa atttcgagct ttttctttga tgtcagcacc
     9721 tagtgattgt gctttccctt ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc
     9781 ttcttttcct tcaccgacta ctttatcttt catatcttta gaacgtcctt ttaaatctgc
     9841 cattgtgctt cctcctctta gtttgtttat tgatctacct tcagggtaac atcaacaaca
     9901 gatacagaca aacagaacgc tcgttttcta aaaaagatag ttagagctgt tgattaaaaa
     9961 cttttttcga aagtccccca tactttgact tctttttctt ggaccattat ttctccttta
    10021 gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc tgcatcagca
    10081 ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt actggttata
    10141 gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt gatccctact
    10201 agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc atcaaaataa
    10261 ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg
    10321 atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc ccaaaaatca
    10381 ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct
    10441 tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat cggaatttct
    10501 gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc taagtggaca
    10561 ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc tactgcacga
    10621 gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat ttctccgatt
    10681 ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac tgtttttacc
    10741 ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc cagacctttc
    10801 gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa acaaccgaca
    10861 actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt tcgattttga
    10921 taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa acccggggtt
    10981 agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc gccttctagc
    11041 tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt tcctaagtaa
    11101 tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc tttctttaag
    11161 tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta ctcctttgat
    11221 tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac ttttttattt
    11281 acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa aatagttcct
    11341 aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc ccattcgatc
    11401 cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa
    11461 cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac taaatctgcc
    11521 agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt aacaaccaag
    11581 ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg aacaccggtc
    11641 aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga tgcatcagat
    11701 cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt accagctaaa
    11761 tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc atctaatttg
    11821 aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga agcgatttct
    11881 ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac aactaacatc
    11941 tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga tgtctttttg
    12001 atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg ttttttacgg
    12061 attttttctg catatatcat catataacca gcggctaatg cagtaacaac tacccacata
    12121 atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg agccaccgct
    12181 acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac ataagtcacg
    12241 gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat caccattgag
    12301 aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa agcaaagaat
    12361 aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc atagatacct
    12421 gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac aaggatcaat
    12481 gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc aaaaacaaaa
    12541 tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt tttatgcaat
    12601 tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc atctgtcaag
    12661 gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat gactttttcc
    12721 aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg cagttcttct
    12781 aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga atcttgacgt
    12841 aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc ttgcgtgctg
    12901 aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac accaaaaata
    12961 atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt tcctgaagaa
    13021 tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat gaataaaaaa
    13081 tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa tttattagaa
    13141 aatcactttt ttctattttg ttttttttct cctatgtatg attatttttt tctctttcca
    13201 tttatattaa gtattttatt tctctgtttt tccttaaaat tcttttttta tttttagatt
    13261 agagaaaatc tcgtcctgtt tttttcattt tacaatcatt gaaaaattga atttcattgc
    13321 aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt tgtgtatttt
    13381 atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg
    13441 caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc
    13501 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc
    13561 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
    13621 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
    13681 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
    13741 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
    13801 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
    13861 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
    13921 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
    13981 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
    14041 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
    14101 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
    14161 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
    14221 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
    14281 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
    14341 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
    14401 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
    14461 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
    14521 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
    14581 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
    14641 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
    14701 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
    14761 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
    14821 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
    14881 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
    14941 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
    15001 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
    15061 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
    15121 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
    15181 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
    15241 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
    15301 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
    15361 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
    15421 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
    15481 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
    15541 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
    15601 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
    15661 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
    15721 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
    15781 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
    15841 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
    15901 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
    15961 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
    16021 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
    16081 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
    16141 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
    16201 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
    16261 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
    16321 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
    16381 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
    16441 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
    16501 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
    16561 gaatgactgc ttttttgacc ttcacttgct
//
LOCUS       DACECJ010000065        16226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.065, whole genome shotgun sequence.
ACCESSION   DACECJ010000065 DACECJ010000000
VERSION     DACECJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.065"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            139..1476
                     /locus_tag="IWO09_002088"
     CDS             139..1476
                     /locus_tag="IWO09_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0264673.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1524..3653
                     /locus_tag="IWO09_002089"
     CDS             1524..3653
                     /locus_tag="IWO09_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0264674.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3628..4317
                     /locus_tag="IWO09_002090"
     CDS             3628..4317
                     /locus_tag="IWO09_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264675.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4725..5411)
                     /locus_tag="IWO09_002091"
                     /pseudo
     CDS             complement(4725..5411)
                     /locus_tag="IWO09_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5547..5741)
                     /locus_tag="IWO09_002092"
     CDS             complement(5547..5741)
                     /locus_tag="IWO09_002092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264676.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5791..6231)
                     /locus_tag="IWO09_002093"
     CDS             complement(5791..6231)
                     /locus_tag="IWO09_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0264677.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6235..7080)
                     /locus_tag="IWO09_002094"
     CDS             complement(6235..7080)
                     /locus_tag="IWO09_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0264678.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7229..8647
                     /gene="pepV"
                     /locus_tag="IWO09_002095"
     CDS             7229..8647
                     /gene="pepV"
                     /locus_tag="IWO09_002095"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0264679.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8704..9651)
                     /locus_tag="IWO09_002096"
     CDS             complement(8704..9651)
                     /locus_tag="IWO09_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0264680.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9874..10224)
                     /locus_tag="IWO09_002097"
     CDS             complement(9874..10224)
                     /locus_tag="IWO09_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0264681.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10424..11503
                     /gene="pepA"
                     /locus_tag="IWO09_002098"
     CDS             10424..11503
                     /gene="pepA"
                     /locus_tag="IWO09_002098"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0264682.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11647..11967
                     /locus_tag="IWO09_002099"
     CDS             11647..11967
                     /locus_tag="IWO09_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0264683.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11989..12453
                     /locus_tag="IWO09_002100"
     CDS             11989..12453
                     /locus_tag="IWO09_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0264684.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12658..13263
                     /locus_tag="IWO09_002101"
     CDS             12658..13263
                     /locus_tag="IWO09_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0264685.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13302..14591)
                     /locus_tag="IWO09_002102"
     CDS             complement(13302..14591)
                     /locus_tag="IWO09_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0264686.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15019..15498
                     /gene="rlmH"
                     /locus_tag="IWO09_002103"
     CDS             15019..15498
                     /gene="rlmH"
                     /locus_tag="IWO09_002103"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0264687.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15701..16226
                     /locus_tag="IWO09_002104"
                     /pseudo
     CDS             15701..16226
                     /locus_tag="IWO09_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5134 a         2971 c         3298 g         4823 t
ORIGIN      
        1 gcataattaa ggttttttaa ttgaaaactg aacatttatg gtcacccttt aatgaaagcg
       61 ctaactacat aagttagaat gagtttgact gatatattag tttgcttatc agaaaaacaa
      121 aatggacgga ggaataacat gagtgatatt attattggta ttttgttagt tatctcattc
      181 tttttcatgg tctggtactg tgtaaaaggc tacaacttga tggtcggctt tgccatcatg
      241 gctactgtat ggatgggatt agcgctagtc ggaaacacgt tttcaccaaa cccggcgatg
      301 gaagggcaag gagtaattga tgtgttgacg catatctata cgacggggcc agctgagtac
      361 gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt tcgggcgtgt tctagtagat
      421 agcggaatcg cagctaccct gattcgaaag gtagttgaac ttggagggga caagccaaga
      481 atcacgatga gcttactatg tattgtaacc gctgttatat tcatgtcgat gacaggaatc
      541 ggaccagtga tttctattgc agttattgtg ttgccaatct tgatgtcatt aggtatttca
      601 gtaccggttg cactgttctc atttatgggc tcgattatgg ctggaatctt tgctaatatc
      661 gtcaatttca aacaatatca aacgatttat gctggtttca atccagcggc tgaaagctat
      721 acttataatg actatttcca aattggtatg attgggatga ttgtaagctt agttgtggta
      781 ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct atgcaatggc agcaaacgta
      841 ccagctgaag gtggcgacgc accaatgatc tcatggttag ccgtattatt accagtttta
      901 ggggtcgttc ttttagatct tccaatcatc ttaggtttta ttctagcagg tatctgggca
      961 ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa tctgtcgtca gtttgcgaaa
     1021 ttatttacag atggcgcagt agatgttgcg ccaatggttg gtttcttgat gaccttagcg
     1081 atgttcaata attcagctgc gtatgcttct ccttattttt cagctatttt tggagattgg
     1141 ataccgcaat caccattagt cttggcaatc gtatttgcta tattgacacc actaggattt
     1201 ttccgcggtc caatgaatct agtcggttca ggctcagcta ttttagctgt cgtattagct
     1261 gttaatccaa cgatgtcgcc agcctttttg ttcccgttat ttgcaatcac aacgattgct
     1321 cctcagcatt tagatattac acaatcttgg gttgcttggg gattaggtta tacaaaagta
     1381 acttctcgag aatatatgaa gaaatccatc ccaactggtt ggatcatcgg agcgatctta
     1441 tgcctgatca cgttcttatt gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga
     1501 ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg
     1561 gtacacatac gaaagcagtc gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt
     1621 cagtgaaaac aacccacgat gatgtccgtg gagtagccgc aggtgtcgtt cagtcttttc
     1681 aaaattgttt gctggaaaat aatatcagtc cagaagatgt tgtgtttgtt gcacattcta
     1741 ccacacaagc gactaatgca ctgattgaag gagacgtagc aaaagtcggc gtaatcggga
     1801 tggcaaaagg tggattagaa ggttttttag caaaacgaca aactagattg aacgatatcg
     1861 atttagggaa taagaaaaaa atagagattg tcaatgcatt cttacctgtc aaacacttaa
     1921 atgtagaccg tgtttcagaa acgatttctt ctttagaaag agaaagagcg gaggtcttgg
     1981 tttcttctat ggcatttgga gtagataatg gggaaccaga acgagttgtt tatgaagcag
     2041 catcggtaaa atcgatccct acaacgatgg cttctgacat cacaaaatta tatggattga
     2101 caagaagaac tcggacagca gcaatcaatg catctatttt accaaaaatg ctagatacgg
     2161 caacctctac agaagattcc gtacgagaag caggggtcaa tgtttctttg atgattatgc
     2221 gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc
     2281 tttccggtcc agcagcatca gtcatgggct ctttgatgta cttgagagcg tctaatggtg
     2341 tttattttga agtaggcgga acaacaacca atatcggggt cattaaaaat ggacgtccag
     2401 caatcgatta ttcaattgta ggtggccacc caacatatat ctcttcatta gatgttcggg
     2461 ttctaggtgt ggcaggcggt tcgatggtac gggctaatca gtcaggaatc atcgatgtcg
     2521 gtccacgttc tgcacatatt gccggtctag attatgcagt atttacggag acagaaaaaa
     2581 tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg agaccctgct gattacgtca
     2641 aggtagtgat ggaagacgga gaagaagtga ctattacaaa tacatgtgca gccaatgtcc
     2701 ttggattagt gcaagaagaa catttttctt atggaaatgt cccatctgca cgaaaagcca
     2761 tccaagcatt agctgattat tgtcatacga cggttgaaga catcgcagaa cagatcatgg
     2821 aaaaatccta tgctaagatt gaaccggtga ttttagaatt ggcagacaag tatcacttgg
     2881 agaaagatca gatttctctt gtaggtgtag ggggcggcgc tgcctcattg attacttact
     2941 ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa cgccgaagtg atttcttcta
     3001 ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg tatcatccct tcgccaagta
     3061 aagaagatat acgatcgttg aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta
     3121 caccagaatc gatcgaagtc cacgtagaaa tcgatccaca gacttcaaaa gtaacggcta
     3181 tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac caaggagatc acaacagaag
     3241 aagcactaga gcttgctgca gaggatatgc gattaaataa aaatgaagtt tgcttattgg
     3301 aaaatacacc tttcttttat gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc
     3361 ttcggatcat cgatcaaaaa ggatttatca aggtccaacg tggacatgct tcatgtatga
     3421 aaacaactgc agccaattat atgacagctg tcgaacaact ttgggaagat atggcagttt
     3481 atcaaactga attgattgcc cgaccagaat tttatctctg cttaggagca agagtctctg
     3541 attttacagc gacggatctg gaacaactac agcttttgat ggatctggaa gtatctacta
     3601 tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat caaacaaaca taagttagaa
     3661 gagttactaa acgaattaca acagatcccc gaagaaattt ggggattcta ccagtttcgg
     3721 cgagatttat tttggaaaaa gattcctttg tccaagcaga aaattcttat acaacaatca
     3781 atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta
     3841 ggagaaattt gtgaacaaat ggctgttcca atcgtttcct gtgaatcaga gcaaataaac
     3901 gaacgaatca cttttgcaac ttatgcagaa gatgaaggga ttcgattgat gacggagccg
     3961 ttagaaaaat taaaatgttc aggactagct tcgatctcta aagaaacagc gcaagctttg
     4021 attattggac atgaactatt tcaccacatc gaagcttctg ttaagggaat ttatacgcaa
     4081 agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac atcaatcaaa cattcgtgca
     4141 ttgagcgaga tagcagcgat gagtttttca aaagagatga atcaaagcga tttttcgcct
     4201 tatgtattag aggcagtctt actttggcca tataatgaaa ttcgtagcca agggatctta
     4261 gaagaaataa aaaaaataga aaaaagatgt acggagtatg atttagatta caaataaaga
     4321 gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa taaacggtgt tctcctgtca
     4381 actcctcggt cacaagtcaa aatattcaac aaacatgaga agctgttttt tgaaattgtt
     4441 ccttgtgact cggagatgaa cggcaggctc acgacctctt ggaaaacggt gttcaaaagc
     4501 ttgcatctgc ggtaataagt ccagccaagt taaaaacata agaagctatt tttacttggc
     4561 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctcaataaac ggtgtgcaat
     4621 cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat atgaggaact attttgccgt
     4681 ttctttctct aatactcagg tcaaaacgct gattccaaaa cctcttattt gatgggtctg
     4741 tcctgaatat cttcaaaata atcggggtaa ttatgaatca cgtattcttg ctcctcgata
     4801 atcatatgta aatgcatcgt gcaaagaaat ccgacttcat caaagttatg atcaatcatt
     4861 gattgaagca ttgtctgatg ttcatctaat actcgacccc agtctaattc tgaaatcgtc
     4921 aaacgtaacc atctgaaacg gttcaagtgc gtactataac tttctagcca atcccaaatt
     4981 tctcgttttc cagcaatttc ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa
     5041 gcttttttat tttgagcgat tactgcctga tcggtttgtt ctagtatttt ttctagttta
     5101 gcttgatctt acttggtcat tttcgcactg cattcttgca ggatcggttt ttccaacttt
     5161 tcccgcgtat aacgggctaa tgaagcagat cggatatcga tttttgaaac ataagtgccg
     5221 gactgtggaa tggtttttac aagctcttga ttgcgcagtt ggatcagtgc ttctcgaatc
     5281 ggtgtacgcc cgatagctag ttgttcttct aacactttat cggagatttt acttccaggt
     5341 gctaactcgg aataaatgat ttgtttacgt atagattcat aagcttgctg ttgcaaatta
     5401 ttgataacca tttaaccttc ctttctatgc agatataatc tattataaca taaatcgctt
     5461 acaaatcgtg tgggcataaa aacacagtct gcaaaaaaag ccctgatagg catgcctatt
     5521 agagcctcga aaagaaaaat attgaatcaa tctggtcgta cctcttggat cggtttgaca
     5581 cgaccgccga ttcttttagc aaaagcttcg gattcttttt tgtctttgaa aatgagcaga
     5641 ttgtctaagt ttttttcccg agtgcgacag ccagttttat cgatgtaacc gtgcattact
     5701 ttaacgacat acatggggat cacctgcttt tttcttttca ttatacgaac tactcgacac
     5761 aatacaagaa aaatgcttag acggtcttta ttaagaattt acatcatcca aagaagcgta
     5821 cgcttggaat aactgatcta actccggatt gactttgctc agcttcacta atcgttgcga
     5881 atcagaaagt aaaaaacagt gtttactact gccagtagtt tttaattttt tgtcttcagt
     5941 tcccacaatt tcgtagctaa aatccaaccg tgtccccgta tacttttgga tagttggatg
     6001 gatgctcact ttttctccgt aatggatcat ttctttatag ctgcaattta cttccagaac
     6061 ggggatgatg atcccagatt cttcgatttt tttataggga aaccctagat gtaccaataa
     6121 ggctacccga gcctcttcaa accagcggat ataattgctg tgatgaataa tccccatttg
     6181 atctgtttca taataaaaag gttctcgcac ataacctgta aatttctcca tgactcatgc
     6241 ctcgttttcc attgattttt tacaaaaata attcatccat ttcggaagtg cttcgctgat
     6301 ttgtgtcggc aaaacaatat attgttcttt tgccagctgg tctccaatga aactatgaag
     6361 ataaatagct gctgcaatcg tttggtcatt ttgaggaaac tgggctaaaa atcctgtgat
     6421 catcccagct aaagtgtcac ccatgccgcc cgttgccata gcagcagaac ctaaaggatt
     6481 ggtgtagaag tgctggtgac cataaatttt tgtctcatga cttttcaaaa cgagcacagc
     6541 accgatttcc tgttgcttct ttttgttttc tttatcccgt tgttcttgta tcggtagaga
     6601 actcaaccgt tgccactcca tttgatgagg tgtaagtaca gtttgttcgg gatagggtag
     6661 caaaagatga ttagcagcaa gtaaacttaa agcagagcca tcaatcacca gccattgttc
     6721 ttttgattgg ttttctagta cttgtttgaa acgttccaaa ctcttagaag aagtacccaa
     6781 tccgggacca atcaagagta catctgcttg ctttattacg gaaagaaact ggtcagtatc
     6841 cgtccaaact acaatcattg cttctggcag atgactgtga agagcactcc gatttttttc
     6901 atcagttact acagtcgtga gacccgctcc gctgtgtacg gcagctaaag cactcataat
     6961 gattgctccg ccatattgtt cattgccgcc aatcaaaacg atacgtccaa atgttccttt
     7021 atgtgaaatt tttggacgag gtgtgatgac tcttttcact agttgttcgt ttatcagcat
     7081 ctaaaaacct ccttttactc attatagcgt atattttttt tagagaaaaa cagataagaa
     7141 aaggaattgt cagatttttt gaactatttt tttctgtaat ttgtggtatc atgaacacga
     7201 agacttaaat aagtggagga ttaactttat gacaattgat tggcaaaaag aagtagaagc
     7261 acgtaaagaa gacttgcttg aagacttgaa aaatctttta cgtgtcaaca gcgagcgcga
     7321 tgactcaaaa gtaacaccag atgcaccatt tggacctgga cctcgcgacg cattgaaaca
     7381 tatgttagct tatggcgaac gtgatggatt taaagtaaaa aatgtcgata actatgcagg
     7441 gcatattgat cttggcgaag gagacgaaac gctagggata tttggacaca tggatgtcgt
     7501 tcctgcagga gatggctggg acacagatcc ttatgaacca gtaatcaagg atggaaagat
     7561 ctttgcacgc ggatcaagcg atgacaaagg tccaagtatg gcagcttact atgcgatgaa
     7621 gatcattaaa gaacttgact tgaaactatc aaaaaaagtt cgcttcgtag tcggcagcga
     7681 tgaagaaagc ggctggggcg atatggctta ttatttcgaa catgaagaag aaccagattt
     7741 tggtttctca ccagatgctg aattcccaat catcaatggt gaaaaaggaa atgtttcttt
     7801 agctttacgc ttcaaaggcg acaatgcagg agattatgtc ttgaaatctt ttgtttccgg
     7861 acttcgcgaa aatatggtgc caggaacagc aactgcagca cttcaagtac caagtgcaga
     7921 tgcagctatc gcgatggaag aagcatttta ccaattcatc gaagcaaatc cagtaagcgg
     7981 aacgattgaa gcggacaata cgtatgtaaa aatcgaatta gttggtaaag gcgcacatgg
     8041 tgcaagccca caatctggta tcaatgcagg ttctttcttg gcattgttct tagataacta
     8101 tgaattccta ggatctgcta aacaatttat ccatgtagca gctgcttatg tccatgaaga
     8161 tttttatgga gaaaaactag gagtcgctta tgaagatgaa aaaatgggca aattgacaat
     8221 gaatgctgga ctatttgctt ttgaagaaaa tggtacagag gaagcgaact tcatcaatat
     8281 gaatttccgt tttccaaaag gtgtaacagt agacggattg caaagtgaca tcgaacaaac
     8341 agtggggcaa gaaggcgcaa cagttactcg aggtgctcgc gtaatggaac cacattacgt
     8401 accaatggat gatccattgg tggctacatt attgcaagtc tatgaagacc acactggaga
     8461 aaaaggctac gaacaaatca tcggtggcgg aacttacgga cgtttgttga aacgtggcgt
     8521 tgcttatgga gcgatgttcc caggatatac tgatacaatg catcaagcaa acgaatttat
     8581 gagtcttgat gacttgttca gagcaacagc aatctatgca gatgccatct atcgtttggc
     8641 gaaataatta attaaaaaga ggatcaagaa ttgcaaatta tttttgcagt cttgatcctt
     8701 tttttacttt ctactgtggt attcatgata aacatgatat agattcaatt catattcttt
     8761 cttagcgttc gtgacgactg tatccaaaat caaccctgtt actaatgaaa gaagtgctaa
     8821 agtcatcaat ccagtactta aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat
     8881 atattcgcga atcacaggaa tccccgtgac caatccaaat aaaaagaaga agatggtcca
     8941 aatcccaaag aaaagcaaag gcttataatc tttaaacatt ttaataatca tcatgatcac
     9001 tttgaatccg tcagagaatg tattcaattt tgattcgctg ccttcaggac gatcacgata
     9061 atcgattggg atttcaacga acttgaattt tttatctaaa gcatggatcg tcaattccgt
     9121 ttcgatttga aaaccagaac tcataatagg aaaacttttg acaaagatac gattgaatcc
     9181 tcgatagccg gtcatgacat caagaatttt tgttttatac attctagtaa ttgtatttct
     9241 aacaaggtta tttccaaaat cgtggaaagg tctcttattc tcatcaaagt aggttccatt
     9301 tgataggcga tcaccaatga ccatatctgc ttcaccagaa cgtaatttct cgagcaaacc
     9361 atgtacagct tctgcaggat aagtatcatc gccatctacc attaaataat agtcggcatc
     9421 gatatcaaag aacatttggc ggatcacatt tccttttcct tgacgaggtt ctttttttac
     9481 gattgctcca ccttgaacgg ccaattcgta tgttcgatcg gtcgaattat tatcatagac
     9541 ataaatatct gcttctggta attctcgttt aaagtctgca atgactgttg aaattgttgc
     9601 ttcctcattg taacaaggaa tcagaacagc aattttttct tgttgttcca tttttcactc
     9661 tccttaacta tagaaaacaa aagctaaatt aaaaatacct cataatcaaa ggaaatgcta
     9721 gtttttcccg ctaaatatca tttatttaaa aagaaaatac tggaatatat gtttggaaag
     9781 ctctatctaa aaaaccaccc actacaaaag aaggacatga tacctctctt ttgaatagtg
     9841 ggtgcgtatt cgttctctca cttttttgac gtattaatct gttgcacctt tttttcttga
     9901 aatttctaat acggttcctg tatgagcatc tgctaaaaat tcataataaa cagtttcgcc
     9961 atcttctatt cgagaaagac ctccccgata agcactcgac cgaacggcga atttcctaat
    10021 aggttccttt tcaaaagaga tccaagaacc ttcgattgca ccttctttta gaaaagatga
    10081 cttgatattt tcaagtactt ggtctgctga caatactttt ttgcgatgat accaaatcgt
    10141 tgaagctacc ccgctcaata atcctagacc gattccaact gctaacccgc ctttaaaata
    10201 gcttagttcg ctttcttcat acatgtggac atacctcctt cttctcactt gttactcctt
    10261 gtgcttattt taccacactc tcatattttc tttgaaataa ataaccgcta ccagaaaaaa
    10321 taagctataa taaatacgat cggtctgcag aaaaaatcat attttatccg cagatcaata
    10381 taagtctgag aggagtttag tctgaaattt agacgaatag catatggaag aaaaaacatt
    10441 tcaacgaatc aaagagttaa cagaattaca aggaacaagc ggttttgaac aagatgttcg
    10501 cgcttatatg gaaagtcata tggaaccgct ggtagatgag ttacagttag atggtttggg
    10561 cggcattttt ggtctgcgcc atcatgaaga agcggatgca ccccgtgtaa tggtcgcagc
    10621 acatatggat gaagtcggat tcatgctgac acaaatccaa gaaaacgggc tttttaaagt
    10681 cgtacctcta ggcggctgga atccttacgt tgtttctgct cagcgtttta cacttaaaac
    10741 ctctactggt aagaattatc catgtatttc ttcttccgtt cccccacatt tattgcgagg
    10801 aacaagcgga caaaaatcag tagaagtaac cgatattttg ttcgatgctg ggtttgaatc
    10861 aagagaagag gcaatgagtt acggtgtcct tccaggagac acaatcgttc cttacgctga
    10921 aacgatcaaa acagccaacg gaaagaatat tatcagtaaa tcatgggaca accgctatgg
    10981 ctgtacaatg gtattagaag ctttagaagc gttgcaaaat gaaacattag gtcatacatt
    11041 gattgcaggc gcgaatgtcc aggaagaagt aggcttgcgt ggttctaaag cttctgtcaa
    11101 taaattcaag ccagatctgt tctttgcagt agactgctct gcagcagatg atacagtgac
    11161 gaaaaatggc acattcggac atttaggaga aggtacattg atgcgtatcc aagatccagg
    11221 tttgatcatg cttcctcgtt tgcgcgagta cttgctggat atcgctgaaa caaacaatat
    11281 cccttatcaa tactttgttt caaaaggcgg tacagatgca ggtgctgctc atacacaaaa
    11341 cgaaggaatc ccaagtacag tgattggcgt agtgggacgt tatatccata cgcatcaaac
    11401 gatgttcagt attcgcgatt ttgaagctgc acgagaaatg ttgattcaaa cactgaaagg
    11461 tttggataaa tcaacagtaa atacgatcgt atacggaaaa taatgcgata gtgaaactat
    11521 aaaatctggc aaaagattgc tttttgccag atttttttta taaaaagagg atcaatgttt
    11581 gttagttcga aagaaaggtg tataatttaa ttgaaaaata cataaacaga aagtagagga
    11641 caaagaatga ttatcccaaa tagtttagaa gaattagcag gctacgtaga aactggaaag
    11701 agtgtatttt tctttactgc cggctggtgc ggagactgcc gttttatcaa accgcaaatg
    11761 cctgaaattg aaaatgactt ttctgattgg caatttattg aagtagaccg tgacaagtac
    11821 atagatgtag ccgctgaatg gaacattttt ggtatcccaa gttttgtcgt gatccaagac
    11881 ggaaaagaat taggtcgtct agtgaataaa gatcgtaaaa caaaacagga aatcgaatcg
    11941 ttcctagaaa gtgtaacaaa aggctgacat ctgttaggag gggtcaagat gaatcaagaa
    12001 tataagaata ttttagtagg tatcgacggt agtgaacaag cacttgctgc tttcaaaaaa
    12061 gcggtagaag tggctcgccg taataatggg aaagtatatg tagcaaacgt aatcgatcag
    12121 caattttata actttatggg ctatgcacct attgatcaaa atctagtcga tcagcaaact
    12181 gaaacagcca aggaaatgat cgaagaatgt aaagaatatg ggaaatctgt tgattacaca
    12241 aatatagaag gaattattgc atatggttct gttcgtgaat ccatggcagt caaattgcca
    12301 aaaaaatatg agatcgatct aatcatggtt gggcagtcag gtctaaatgc agtcgaacgc
    12361 tttatgacag gaagtgtagc cagctacgtg atacgtcatt caccttgtga tgtcttgatc
    12421 gtttctgacg aagaagaaca gccaagtaat taagcaagtg gaacttgtac gaccttttta
    12481 atcaattaac taaaacagta aagggactgt gacaaaagtc ttgtcacagt cccttgttac
    12541 gaataaatgg tgttccagaa gaggcttctt cggaaataaa gcatgaaaaa aatttcatgc
    12601 agtgaatgca attttttgtt aagataaatc tcagcaaagg ataatggagg aaagaacatg
    12661 atttttgctt ataacaaagc acacgtcgga gacaccttga tggttattat agccgacgat
    12721 aaaggaacag aaaacactgt tgaacgcaaa tggaatgttg ctcggatcaa agatgaacaa
    12781 ggtcaagttg ttgcttggaa ctttttccat atctccgatc atctaacaat cgaagggaac
    12841 ggccaagtaa gcttaacaga tgaggaatta gcgaaattga atcgattgat caaagaagca
    12901 ggattcgaag aacagcttga agctgatcat gaaccgaaga tcgttgtcgg gtacgtaaaa
    12961 acatgcgtcc cacatcctga ttctgaccat ttatcgatta ctgagacaga agtagacaat
    13021 ggtacagtat tgcaaatcgt ttgcggtgca cccaatatcg aagctggaca aaaagttgtc
    13081 gttgcaaaac caggagctat gatgccagat ggaatgatga tctggccagg aactttgcga
    13141 ggggtagata gctatggaat gatctgttcc gctcgtgaat tgcatttgcc aaatgctcca
    13201 gcaaaaaaag gaatcttagt attgcctgag gatgccgtag tcggagaagc atttccaaaa
    13261 taaagaggtt gtgacaagtc tttgtcacaa cctctttttt attaattttc actattttga
    13321 ttttgtttta atgccgattg atcgatcgtc aattcaacag tagttgtttt ttctttcgag
    13381 ccttcatagt aagtgatttt gacagaatct cccacttttt tcttatacaa agcagattga
    13441 agttctacac cagaagaaac ttctttgtca tcgatcttag taatgacgtc gtattgtttc
    13501 aatccagctt tttcagccgg tgtagctgtt tggacatttg tcacgatgac accgttagtt
    13561 acagatgaag gaatcttcaa gatttgttct tgttgctgag tagaaacggc agataagtcg
    13621 accattgtga tacctagagc tggacgtgtc acttttccgt ctttttccag ttgattgatg
    13681 atattcacta catcgttact tggaatagca aatcccattc cttctacgct tacattagaa
    13741 gtagattcag aagtacttgc gatcttactt gagttgatac cgataacttg tccttcgata
    13801 ttaactaaag gaccaccaga atttccaggg ttgattgctg catctgtttg gatcgcattg
    13861 atattgactg tttcgttcga ttcattcgta cttgtcactt gtcggttcaa ggaagaaatg
    13921 attcctgaag tgacagaatt tgcatattca gatcctagtg gggaaccaat cgcaatcgca
    13981 ggctcaccaa ccttcagagc acttgagtca ccaaaagagg caactgtttc gactttgtct
    14041 gaattgatct tcaacaccgc taaatctgaa taagcatctg ttccaactaa ttcagctttt
    14101 accttcgtac cgtctttcat caagacttca agaccttgct ggccatctac tacgtgatta
    14161 ttcgtgacta cataagcagt attcccgtct tttttgtaga tcacgccgct tccttcacta
    14221 taagcttcca aattactatc atctgagctg ctactttctt cttgctgccc gaatagtccg
    14281 ccaaaaccgc ttgattggtt ttgactttgt aagttgataa cagaaacgac agcgtcttga
    14341 actttatcta ccgctttggt gatatcagaa tctacattga ctttgacatt ttccacaact
    14401 gtttcaccgg cagaattctg attgccgctt gtagcagtag aactattgtt gccagtgccc
    14461 atcgctgcat aaaaaatgcc agctgtcagt agtcctccaa cgatccctcc aacaagcccc
    14521 agacctaact tacgccagag gctattattt ttactttttt tcatctttgg tgtcacattt
    14581 tttctatcca tgtgttcctc ctccaattac attcatttat ctactgttag tatagtctta
    14641 tttgttgact ttagtctact ttgagactgt gaacttttta tgaaaaactt taggaaaaat
    14701 cattgttttc agatgaaaat atttgatttt ctttttccac agatactgtg taaaactacg
    14761 cggataggat tggaaaaata agggataaaa catatccaca gcctgtgtat aaagtggata
    14821 aattcttata aaacgtatgt ttgcctaatg aatgtgatga aataaggtaa aataacactg
    14881 ttttctgtgg ataaacctgt ggatgttgtg cataactgtt ggggaaatct ttttaacaaa
    14941 aatagccaaa ataaacatat agacgaaaaa attgattata ataaacaaag tgagcatgaa
    15001 gaccaaagga gagaaaacat gaatatcaaa attatctcag taggaaaatt aaaagaaaaa
    15061 tacctgatcc aaggtatcaa tgagtatgtc aaacggctga atgcctatgc aaaaatcgaa
    15121 ttgatcgaag ttcctgacga aaaagccccg gagaatttaa gcgaagcgca aatgagacaa
    15181 gtaaaagaaa aagaagggga acggatctta gcgaaaatca aagaacaaga atacgtctat
    15241 gccttagcaa ttgaagggaa aaatcctaca agtgaatctt ttgctaaaca aatcgaccaa
    15301 ttaggtatcc aaggaaaaag ccaccttgtt tttgttatcg gcggctcttt aggactcagc
    15361 gaagccgtca tgaaaagaag caatgctcag atctcttttg gaaaaatgac gtatccacat
    15421 caattgatgc gtctgatcct tgtcgagcaa atctaccgtg ccttccggat caatgcgggg
    15481 gctccatacc acaaataatg atatttttta agagaaaaaa ttatccctat tttaaataat
    15541 agcaataggt agtggtaggg agaaatttat aaataacttt tttatttgct ttggtagcca
    15601 agtaagttgc aaaagaaaaa tagatactgt taaaaagata atgtagacat agaaacaaat
    15661 acgtaaaatt ttagttagaa taatggcaag gagaaaaaac atgatagatg aaaaaaagtg
    15721 tttaaaaaat ctattagagg atatggatga tattgaagaa aactatctaa atggactggg
    15781 aaaacaaata tatttcagat acttaagcta tctaaaaatg aaataagaca tagtaatttt
    15841 ttagcttttt tgtttgctcc aaatgaaacc cacaatttat ctgatgagtt ttttaaaatg
    15901 tttttgaaaa ggtatataga ttctaatgat gataccaaag ctgctatcaa ttattttgat
    15961 gctttactta atagctatga agatttaatt gtttacagag aaaataagaa tattgatatt
    16021 ttattggtat cggaaaaaaa taaaatagtg gtgtgcatcg aaaataaaat tctgtcttcg
    16081 gagtctggag ggcaacttaa taaataccaa atatatatag aacgcaatta ttctaactat
    16141 aaaaaaattt ttgttttttt aactccagat ggaaatgaac ccacaaatcc tagttggggc
    16201 ataattactt acaagaatat cgttga
//
LOCUS       DACECJ010000066        15687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.066, whole genome shotgun sequence.
ACCESSION   DACECJ010000066 DACECJ010000000
VERSION     DACECJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.066"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            68..751
                     /locus_tag="IWO09_002105"
     CDS             68..751
                     /locus_tag="IWO09_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0264688.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IWO09_002106"
     CDS             753..1589
                     /locus_tag="IWO09_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0264689.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IWO09_002107"
     CDS             1600..3354
                     /locus_tag="IWO09_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264690.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="IWO09_002108"
     CDS             complement(3630..3971)
                     /locus_tag="IWO09_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0264691.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="IWO09_002109"
     CDS             complement(4039..6210)
                     /locus_tag="IWO09_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0264692.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="IWO09_002110"
     CDS             6421..7278
                     /locus_tag="IWO09_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0264693.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="IWO09_002111"
     CDS             complement(7349..8206)
                     /locus_tag="IWO09_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0264694.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="IWO09_002112"
     CDS             complement(8191..10893)
                     /locus_tag="IWO09_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0264695.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="IWO09_002113"
     CDS             complement(10906..12195)
                     /locus_tag="IWO09_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0264696.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="IWO09_002114"
     CDS             12345..13391
                     /locus_tag="IWO09_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0264697.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="IWO09_002115"
     CDS             13393..15546
                     /locus_tag="IWO09_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0264698.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4454 a         3282 c         2887 g         5064 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DACECJ010000067        15230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.067, whole genome shotgun sequence.
ACCESSION   DACECJ010000067 DACECJ010000000
VERSION     DACECJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.067"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWO09_002116"
     CDS             278..787
                     /locus_tag="IWO09_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0264699.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWO09_002117"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWO09_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0264700.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWO09_002118"
     CDS             2322..3302
                     /locus_tag="IWO09_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0264701.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IWO09_002119"
     CDS             3424..4842
                     /locus_tag="IWO09_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0264702.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IWO09_002120"
     CDS             4913..6382
                     /locus_tag="IWO09_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0264703.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IWO09_002121"
     CDS             6392..6859
                     /locus_tag="IWO09_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0264704.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IWO09_002122"
     CDS             6895..7767
                     /locus_tag="IWO09_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0264705.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="IWO09_002123"
     CDS             7848..10382
                     /locus_tag="IWO09_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0264706.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IWO09_002124"
     CDS             10538..11311
                     /locus_tag="IWO09_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0264707.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IWO09_002125"
     CDS             11304..12095
                     /locus_tag="IWO09_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0264708.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IWO09_002126"
     CDS             12105..12596
                     /locus_tag="IWO09_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0264709.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IWO09_002127"
     CDS             12614..13024
                     /locus_tag="IWO09_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0264710.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IWO09_002128"
     CDS             13028..14428
                     /locus_tag="IWO09_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0264711.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="IWO09_002129"
     CDS             14451..14825
                     /locus_tag="IWO09_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0264712.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DACECJ010000068        14815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.068, whole genome shotgun sequence.
ACCESSION   DACECJ010000068 DACECJ010000000
VERSION     DACECJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.068"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(296..1024)
                     /locus_tag="IWO09_002130"
     CDS             complement(296..1024)
                     /locus_tag="IWO09_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0264713.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IWO09_002131"
     CDS             complement(1002..2576)
                     /locus_tag="IWO09_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0264714.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IWO09_002132"
     CDS             2805..3743
                     /locus_tag="IWO09_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0264715.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IWO09_002133"
     CDS             3755..4666
                     /locus_tag="IWO09_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0264716.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IWO09_002134"
     CDS             4690..6162
                     /locus_tag="IWO09_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0264717.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="IWO09_002135"
     CDS             6213..6824
                     /locus_tag="IWO09_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0264718.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="IWO09_002136"
     CDS             6854..8767
                     /locus_tag="IWO09_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAQ0264719.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="IWO09_002137"
     CDS             8895..11876
                     /locus_tag="IWO09_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAQ0264720.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="IWO09_002138"
     CDS             11873..13534
                     /locus_tag="IWO09_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAQ0264721.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="IWO09_002139"
     CDS             13574..14506
                     /locus_tag="IWO09_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0264722.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DACECJ010000069        14779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.069, whole genome shotgun sequence.
ACCESSION   DACECJ010000069 DACECJ010000000
VERSION     DACECJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.069"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(147..773)
                     /locus_tag="IWO09_002140"
     CDS             complement(147..773)
                     /locus_tag="IWO09_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264723.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1068..1403)
                     /locus_tag="IWO09_002141"
     CDS             complement(1068..1403)
                     /locus_tag="IWO09_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0264724.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1390..1674)
                     /locus_tag="IWO09_002142"
     CDS             complement(1390..1674)
                     /locus_tag="IWO09_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0264725.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1730..3253)
                     /locus_tag="IWO09_002143"
     CDS             complement(1730..3253)
                     /locus_tag="IWO09_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0264726.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3246..4421)
                     /locus_tag="IWO09_002144"
     CDS             complement(3246..4421)
                     /locus_tag="IWO09_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0264727.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4425..4610)
                     /locus_tag="IWO09_002145"
     CDS             complement(4425..4610)
                     /locus_tag="IWO09_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264728.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4576..6270)
                     /locus_tag="IWO09_002146"
     CDS             complement(4576..6270)
                     /locus_tag="IWO09_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0264729.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6267..6740)
                     /locus_tag="IWO09_002147"
     CDS             complement(6267..6740)
                     /locus_tag="IWO09_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ0264730.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6809..6964)
                     /locus_tag="IWO09_002148"
     CDS             complement(6809..6964)
                     /locus_tag="IWO09_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ0264731.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7098..7478)
                     /locus_tag="IWO09_002149"
     CDS             complement(7098..7478)
                     /locus_tag="IWO09_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0264732.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7482..7697)
                     /locus_tag="IWO09_002150"
     CDS             complement(7482..7697)
                     /locus_tag="IWO09_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264733.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7700..8107)
                     /locus_tag="IWO09_002151"
     CDS             complement(7700..8107)
                     /locus_tag="IWO09_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ0264734.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8373..9848)
                     /locus_tag="IWO09_002152"
     CDS             complement(8373..9848)
                     /locus_tag="IWO09_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ0264735.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9838..10686)
                     /locus_tag="IWO09_002153"
     CDS             complement(9838..10686)
                     /locus_tag="IWO09_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ0264736.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10723..11085)
                     /locus_tag="IWO09_002154"
     CDS             complement(10723..11085)
                     /locus_tag="IWO09_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264737.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11385..12009)
                     /locus_tag="IWO09_002155"
                     /pseudo
     CDS             complement(11385..12009)
                     /locus_tag="IWO09_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12006..12317)
                     /locus_tag="IWO09_002156"
     CDS             complement(12006..12317)
                     /locus_tag="IWO09_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264738.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12361..12654)
                     /locus_tag="IWO09_002157"
     CDS             complement(12361..12654)
                     /locus_tag="IWO09_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264739.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12848..13495
                     /locus_tag="IWO09_002158"
     CDS             12848..13495
                     /locus_tag="IWO09_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0264740.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13556..14701
                     /locus_tag="IWO09_002159"
     CDS             13556..14701
                     /locus_tag="IWO09_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0264741.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         4266 a         3045 c         2347 g         5121 t
ORIGIN      
        1 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
       61 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
      121 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
      181 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
      241 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
      301 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
      361 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
      421 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
      481 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
      541 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
      601 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
      661 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
      721 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
      781 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
      841 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
      901 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
      961 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
     1021 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
     1081 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
     1141 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
     1201 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
     1261 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
     1321 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
     1381 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
     1441 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
     1501 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
     1561 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
     1621 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
     1681 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
     1741 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
     1801 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
     1861 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
     1921 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
     1981 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
     2041 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
     2101 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
     2161 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
     2221 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
     2281 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
     2341 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
     2401 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
     2461 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
     2521 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
     2581 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
     2641 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
     2701 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
     2761 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
     2821 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
     2881 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
     2941 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
     3001 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
     3061 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
     3121 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
     3181 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
     3241 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
     3301 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
     3361 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
     3421 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
     3481 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
     3541 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
     3601 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
     3661 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
     3721 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
     3781 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
     3841 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
     3901 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
     3961 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
     4021 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
     4081 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
     4141 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
     4201 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
     4261 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
     4321 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
     4381 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
     4441 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
     4501 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
     4561 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
     4621 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
     4681 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
     4741 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
     4801 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
     4861 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
     4921 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
     4981 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
     5041 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
     5101 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
     5161 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
     5221 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
     5281 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
     5341 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
     5401 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
     5461 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
     5521 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
     5581 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
     5641 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
     5701 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
     5761 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
     5821 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
     5881 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
     5941 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
     6001 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
     6061 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
     6121 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
     6181 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
     6241 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
     6301 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
     6361 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
     6421 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
     6481 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
     6541 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
     6601 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
     6661 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
     6721 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
     6781 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
     6841 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
     6901 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
     6961 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
     7021 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
     7081 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
     7141 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
     7201 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
     7261 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
     7321 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
     7381 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
     7441 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
     7501 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
     7561 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
     7621 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
     7681 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
     7741 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
     7801 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
     7861 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
     7921 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
     7981 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
     8041 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
     8101 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
     8161 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
     8221 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
     8281 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
     8341 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
     8401 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
     8461 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
     8521 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
     8581 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
     8641 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
     8701 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
     8761 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
     8821 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
     8881 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
     8941 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
     9001 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
     9061 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
     9121 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
     9181 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
     9241 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
     9301 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
     9361 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
     9421 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
     9481 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
     9541 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
     9601 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
     9661 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
     9721 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
     9781 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
     9841 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
     9901 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
     9961 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    10021 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    10081 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    10141 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    10201 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    10261 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    10321 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    10381 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    10441 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    10501 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    10561 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    10621 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    10681 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    10741 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    10801 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    10861 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    10921 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    10981 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    11041 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    11101 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    11161 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    11221 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    11281 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    11341 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    11401 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    11461 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    11521 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    11581 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    11641 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    11701 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    11761 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    11821 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    11881 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    11941 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    12001 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    12061 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    12121 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    12181 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    12241 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    12301 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    12361 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    12421 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    12481 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    12541 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    12601 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    12661 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    12721 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    12781 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    12841 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    12901 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    12961 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    13021 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    13081 attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt
    13141 gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg attctaatct
    13201 ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt
    13261 aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac
    13321 cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa
    13381 tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct
    13441 tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata
    13501 aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc
    13561 aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg
    13621 aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa
    13681 agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa
    13741 agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac
    13801 tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa
    13861 atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag
    13921 ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc gtatttttag
    13981 ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa
    14041 aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac
    14101 cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt
    14161 actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc
    14221 agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa
    14281 tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga
    14341 aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact
    14401 tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca
    14461 cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt
    14521 gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga
    14581 agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt
    14641 ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta
    14701 gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa
    14761 ggtttctcct agctttata
//
LOCUS       DACECJ010000070        14525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.070, whole genome shotgun sequence.
ACCESSION   DACECJ010000070 DACECJ010000000
VERSION     DACECJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.070"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(330..692)
                     /locus_tag="IWO09_002160"
     CDS             complement(330..692)
                     /locus_tag="IWO09_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264742.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="IWO09_002161"
     CDS             complement(766..1146)
                     /locus_tag="IWO09_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0264743.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1228..2403
                     /locus_tag="IWO09_002162"
     CDS             1228..2403
                     /locus_tag="IWO09_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0264744.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2480..3064)
                     /locus_tag="IWO09_002163"
     CDS             complement(2480..3064)
                     /locus_tag="IWO09_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0264745.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="IWO09_002164"
     CDS             3265..4263
                     /locus_tag="IWO09_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0264746.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="IWO09_002165"
     CDS             4375..4884
                     /locus_tag="IWO09_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0264747.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="IWO09_002166"
     CDS             complement(4914..5573)
                     /locus_tag="IWO09_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0264748.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="IWO09_002167"
     CDS             complement(5575..6339)
                     /locus_tag="IWO09_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0264749.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="IWO09_002168"
     CDS             complement(6347..7024)
                     /locus_tag="IWO09_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0264750.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="IWO09_002169"
     CDS             complement(7061..8446)
                     /locus_tag="IWO09_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0264751.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="IWO09_002170"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO09_002170"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="IWO09_002171"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="IWO09_002171"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0264752.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="IWO09_002172"
     CDS             9385..10242
                     /locus_tag="IWO09_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0264753.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="IWO09_002173"
     CDS             complement(10323..10658)
                     /locus_tag="IWO09_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264754.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="IWO09_002174"
     CDS             complement(10725..12095)
                     /locus_tag="IWO09_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0264755.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="IWO09_002175"
     CDS             complement(12173..12709)
                     /locus_tag="IWO09_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0264756.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13018..14499
                     /locus_tag="IWO09_002176"
     CDS             13018..14499
                     /locus_tag="IWO09_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0264757.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4475 a         2891 c         2358 g         4801 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat tattgataat
    12781 tatcaaaaca tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa
    12841 tatcttgaca aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta
    12901 aataacgtta tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact
    12961 ttccactcac agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg
    13021 ctaattgaag caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat
    13081 tgttctttac aagaattatc caatgactta catattccaa aacggacaat caaagaactg
    13141 atccgaaaaa taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct
    13201 aatcataaag gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt
    13261 tctaagttaa aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta
    13321 atcaattatc cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg
    13381 ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta
    13441 cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc
    13501 tatctttact tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt
    13561 gaattaaatg cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa
    13621 gaaaaaaata tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat
    13681 tttccaaaaa cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat
    13741 tattcagaat actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag
    13801 agagcctact ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa
    13861 aaacaggaga ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt
    13921 caattgatga cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca
    13981 atttattttt attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc
    14041 agtagttttt ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata
    14101 caggaaaaga aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac
    14161 caaatcagtc aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag
    14221 cagttactca tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc
    14281 catttgcagc agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct
    14341 gatttgatca ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg
    14401 gatacaacgc acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat
    14461 atgaaaaaag gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc
    14521 ttatt
//
LOCUS       DACECJ010000071        14519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.071, whole genome shotgun sequence.
ACCESSION   DACECJ010000071 DACECJ010000000
VERSION     DACECJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.071"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(143..1345)
                     /locus_tag="IWO09_002177"
     CDS             complement(143..1345)
                     /locus_tag="IWO09_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0264758.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="IWO09_002178"
     CDS             complement(1348..1542)
                     /locus_tag="IWO09_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0264759.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="IWO09_002179"
     CDS             complement(1589..2437)
                     /locus_tag="IWO09_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAQ0264760.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="IWO09_002180"
     CDS             complement(2642..2992)
                     /locus_tag="IWO09_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264761.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="IWO09_002181"
     CDS             3587..4033
                     /locus_tag="IWO09_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0264762.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="IWO09_002182"
     CDS             4657..5649
                     /locus_tag="IWO09_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0264763.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="IWO09_002183"
     CDS             5665..6549
                     /locus_tag="IWO09_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0264764.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="IWO09_002184"
     CDS             6598..8205
                     /locus_tag="IWO09_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0264765.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="IWO09_002185"
     CDS             8206..9690
                     /locus_tag="IWO09_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0264766.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="IWO09_002186"
     CDS             9867..10859
                     /locus_tag="IWO09_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0264767.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="IWO09_002187"
     CDS             10911..11780
                     /locus_tag="IWO09_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0264768.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IWO09_002188"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IWO09_002188"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0264769.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13820..>14519)
                     /locus_tag="IWO09_002189"
     CDS             complement(13820..>14519)
                     /locus_tag="IWO09_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAQ0264770.1"
                     /translation="DRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNA
                     EIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEP
                     LKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTI
                     SSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNF
                     RARIYLQQGLIFEN"
BASE COUNT         4431 a         2464 c         3010 g         4614 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt
    13801 ctgtgcgccg catatttttt tagttttcaa agatcagacc ttgttgtaaa tagatccgtg
    13861 cccgaaagtt ataaaagttg cgataaccaa acgcaattcg tttaatcagc ttgatcttgt
    13921 tattcagccc ctcagtgacg ccgtttgagt gggacacctt aaaggcattt gaaatacccg
    13981 attggtagcg atggaagatc gtgaatttct ttttaaacga ctgaggtaac gtagatgaaa
    14041 ttgttttaag ggtttcttga aagccttccg tacctcgttt aacgaaataa tagcgtaata
    14101 attgacaaaa atgatacgca gttcgcaatt cttcgttgta gcttaacagc tcatcaacga
    14161 tttctgtttg actaattggt cgtttaaaca gtcgatgata atgccgtttg agtggctcca
    14221 atgtcgtgga atctttgagt aagagtttcc aatatcgctt gagccgacgg tacttcttct
    14281 gattttcaga gtgtgaggtt cgaaaacgat tcatgatctg gacacgtaat tgattgaagc
    14341 tgcgattgat gtgttggaca atatgaaagc ggtcagttac gatctccgca tttggaaaga
    14401 ctgttttaag caactgacaa tagctggcat tcatgtccat cactaggtac ttgaccttta
    14461 aacgggcctt tctaggaaag cgcaggaagt attgagttag tttgtaaagt ctgcgatct
//
LOCUS       DACECJ010000072        14107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.072, whole genome shotgun sequence.
ACCESSION   DACECJ010000072 DACECJ010000000
VERSION     DACECJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.072"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IWO09_002190"
     CDS             66..767
                     /locus_tag="IWO09_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0264771.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IWO09_002191"
     CDS             764..1885
                     /locus_tag="IWO09_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0264772.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IWO09_002192"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IWO09_002192"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0264773.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IWO09_002193"
     CDS             3264..4172
                     /locus_tag="IWO09_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0264774.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IWO09_002194"
     CDS             4207..4479
                     /locus_tag="IWO09_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0264775.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IWO09_002195"
     CDS             4490..5536
                     /locus_tag="IWO09_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0264776.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IWO09_002196"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IWO09_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0264777.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IWO09_002197"
     CDS             complement(8547..9218)
                     /locus_tag="IWO09_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264778.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IWO09_002198"
     CDS             complement(9211..9705)
                     /locus_tag="IWO09_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0264779.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IWO09_002199"
     CDS             complement(9725..10945)
                     /locus_tag="IWO09_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0264780.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IWO09_002200"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IWO09_002200"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0264781.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14103)
                     /locus_tag="IWO09_002201"
     CDS             complement(13021..14103)
                     /locus_tag="IWO09_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0264782.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DACECJ010000073        14018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.073, whole genome shotgun sequence.
ACCESSION   DACECJ010000073 DACECJ010000000
VERSION     DACECJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.073"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..806)
                     /locus_tag="IWO09_002202"
     CDS             complement(150..806)
                     /locus_tag="IWO09_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0264783.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            999..1277
                     /locus_tag="IWO09_002203"
     CDS             999..1277
                     /locus_tag="IWO09_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264784.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1296..2504
                     /locus_tag="IWO09_002204"
     CDS             1296..2504
                     /locus_tag="IWO09_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0264785.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2652..3017)
                     /locus_tag="IWO09_002205"
     CDS             complement(2652..3017)
                     /locus_tag="IWO09_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0264786.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3164..3937)
                     /locus_tag="IWO09_002206"
     CDS             complement(3164..3937)
                     /locus_tag="IWO09_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0264787.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4111..4971)
                     /locus_tag="IWO09_002207"
     CDS             complement(4111..4971)
                     /locus_tag="IWO09_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0264788.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4971..6389)
                     /locus_tag="IWO09_002208"
     CDS             complement(4971..6389)
                     /locus_tag="IWO09_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0264789.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6446..6895)
                     /locus_tag="IWO09_002209"
     CDS             complement(6446..6895)
                     /locus_tag="IWO09_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0264790.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7340..7603)
                     /locus_tag="IWO09_002210"
     CDS             complement(7340..7603)
                     /locus_tag="IWO09_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0264791.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7702..8997)
                     /locus_tag="IWO09_002211"
     CDS             complement(7702..8997)
                     /locus_tag="IWO09_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0264792.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8994..10265)
                     /locus_tag="IWO09_002212"
     CDS             complement(8994..10265)
                     /locus_tag="IWO09_002212"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0264793.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10430..11299)
                     /locus_tag="IWO09_002213"
     CDS             complement(10430..11299)
                     /locus_tag="IWO09_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0264794.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11591..13201)
                     /locus_tag="IWO09_002214"
     CDS             complement(11591..13201)
                     /locus_tag="IWO09_002214"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0264795.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3990 a         2956 c         2394 g         4678 t
ORIGIN      
        1 aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc
       61 tcaaaaaacg gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca
      121 aaacgctgat ttcacaacct ctttgttcat caaaagataa tattgtcttc taacaagtcc
      181 atattcaaaa taacgatatg atggtcttta acggtgataa taccatcttt tttcagttgt
      241 tttaaaattc tgtttacact gctagcagtt gagatgccac aaaatttggc gatttcttca
      301 ttcgtgatcg aaaaatcgat cagcatccct tcttctgtcc gcacaccgaa taaatcatat
      361 aattcgtaaa gttgcgtaca aacagctccc agctttccgt ttaatagcat ttgctgcatt
      421 tttttcatcg aataaagcag acgatagcgg taatactctt ttacatagtg ttgtagcttt
      481 gggtcttggt tgatattttg ccaaaagcgc acacgattga ttcgataaaa ctctgcatat
      541 tctgattcaa ctcgaatatt atacggcgaa tcgatgaatt gtgaatattc gtcttttaaa
      601 agagagacga tttcaagatc tttgatatag cgaaggttaa attctctccc atcatttgaa
      661 ataacacttg cttttaccgt tccctctttc aagatatacg tatgggaatc ttccatccct
      721 tcgaatgtca aatatttatg atatcctttg cgtataatcg gaaaatcatt tttatccaaa
      781 tattccttta agactctact atccaatgat gctcccccat ccaaactttc atctaaaaag
      841 atcatacagt actttaaaac tttttaaagt ccaaaggtat tttactaatg atattaacat
      901 ttgttaagta cctttttcca tcacttatgc gatttttttc ctttttttta ttcgttatac
      961 tgcagaagta aaatttttta agaatagagg gaaaaaacat gaattactgg atgaacacaa
     1021 tcattaatcg attagaaaca gcatatcaaa caagatttga tatgaaagct tctttagtct
     1081 ttttaaatga tgcgtatcaa aactcgatcg aattgatcaa agcagtggat gagaatccaa
     1141 caaacgaatg cgaagaattt ttaaatctgt ttatgtctac aagagattta tttattcgcc
     1201 aacttgttga tcgctatcca tccaattatc acgatgtaga agttcaaata caaaaattaa
     1261 aagcatattc agcttaaggg gaatgtataa catacatgga aaagaaatta tttaataaat
     1321 attttattgg aatcacattg atcaatttta ttgtctacct gatttattat ctgttgatgg
     1381 tcatcatggc agtcattgcg caagacacct tacacgccag cttgagccaa gcaggttttg
     1441 cttctgggat ctatatcatc ggaacattgc tggcacgctt attcatgggg aaacagttag
     1501 aactatttgg tcggaagttt accttaagag gcggcgcgat cttctatctc ctttcaacat
     1561 tagcatatct ttatacgcca actatcgggg cattgtattt agttcgattc ttgaatggat
     1621 tcggctacgg tacagtttct acagctacaa atgctatcgt tactgcatat atccctgcat
     1681 ctaaaaaagg ggaaggcatc aattattatg gattaagtac tagtttagct gccgctgttg
     1741 ggccattttt aggaatgatt ttgctgaata tgactgattt tcgtttcatt atctggtttt
     1801 caattgtact ggtcttcttt gttacgattg cctgccttgt ctttcctgta aaaaatattc
     1861 cattatctga agaacaaaaa atatctcttc gctcatggaa tatcaatagt tttattgaaa
     1921 agaaagcact tttcattaca ggaattgcct ttttgatggg tctttcttat tcaagtgttt
     1981 tatcattttt atcttcttat acaaaagtaa tccacttagt tgctgctggg tcatttttct
     2041 tcgtcgtata tgctttggtc atcacattta cacgtccatt gactggaagg attttcgatg
     2101 tcaaaggaga acaatacgtg atgtatccta gctatttatt cttaacagca ggattatttt
     2161 tactgagtgt aacgacgaat agtgtgacat tgcttatctc tggagcatta gtcggcctag
     2221 gttatggtac gtttatgtca aatggacagg ctgtttgttt aaaaatcgtc cgcgaacatc
     2281 gaatcagtat tgctttatct acctacttta ttggtttaga cctaggctta ggcgttggtc
     2341 cttatttgat ggggatgcta aaatctctta cttcatttag aggattgtat gtgattgccg
     2401 gagtcattcc attgatatgt acagcactct atcttgttaa tggccgaaaa catagtcatg
     2461 aagcagaggg cgaattgatt gaatcattga aaaatgaaat ttgatcttga tgaatagata
     2521 aaaaaaaccg ttcagatctg aaaataatct gagcggtttt ttgagtaaaa atagtttatg
     2581 attattctta attaaacatc tatttatttt taaagctttc atttatcgat aagaatagtt
     2641 taactgttga tttattccat tttccgttca tctggtacga tgccttgact tagcaaccac
     2701 ttgtggaaga accattctgc cacaccgatc aatagagcag gaatcaatac tttcatccaa
     2761 atatcgactg tatcattcat aaaatataga tagagccaca agataactgc agctgaacca
     2821 gcatctgcga tcgtcgctgc gatgttccct actttacgta agatcaacag atcccctagc
     2881 acatagatca tgactacaat aattacccca acaatcgttg aatccataaa gctaacaccg
     2941 tataaacctg tcagcacgac ccacagaatc gcccagatca tgattgcctt cactaccaac
     3001 gccttcacat gtttcatttt ctgcactcct ttctcctgga taaaaaagta agattatcta
     3061 acaagataac cttttcatta ttataccatc ggctgagaga agcgagcaaa gataaggtcc
     3121 tatctaaaaa tgaggaagcc acttagaaca ttcacgtaat aatttatccg tacagacatt
     3181 cgatatggtg ttcctttact ttcgcatact gttcttttgt cagttcttta cttgtcacca
     3241 gataatcgat ttcatgaaaa gcaatggctt gatgcatgaa tttatgtgca attttttgct
     3301 gtgtaactaa aacggcaagc cggtctgatt gttttctaaa agcttttttg accattgcct
     3361 ctccttcatg agtttctgtt actccttggc tgaaatctag cccgcgacaa ctcattatcg
     3421 ctaaatccgc ataatatgac aatgcatcgt tataggcttt tgaaccgttg attgttgctc
     3481 gtttcggaac aattgcccct cccaatacag atacttttgc cgttgtatat tcactcaaca
     3541 tagcagctgt gacgatgcca ttcgtaatga tctgcaaacc atcaaatctt gctaggtttc
     3601 tgactaagtg taaagaggtt gtactggaat ccaagaataa gaccatatca tctcgaatca
     3661 aagaagaagc tgtatctgca atcttacgtt tttctgtttc ttcttctaat ttggtgatct
     3721 tcatcaggta aggcacgtca atttttcgat tgtcaataag catggcgcca ccgcgaactc
     3781 tacggatcaa tccacttttt tccagaaaaa caaggtcacg tttaatcgtg gcttcgctat
     3841 aatttactaa ctcgatcaaa tcggtgactc ttgcaaattg gttgtttttt agataattca
     3901 caatctcctg ctgtctattc tccacttcat tcaacataaa atcaccccat ttactttata
     3961 tagtaacagg aaaaaagagg ttgtgacaga agtgattggc ttcgagaaat aagaagaaat
     4021 ttttcgaaaa aactacttct gaacatcgtg tgtggttaag agggtatgac aagactattg
     4081 tcataccctc ttgaccacac atttttattt ttagtataaa ctagccttgc ctactgtgtt
     4141 caaaatttcc attttatgtt tgaccacttc ttgtaccttt ttattagctg ctggataaat
     4201 tgcattcggc tcgtacatcg atggattttc actcaatatt tctctcaaag cattgaaaaa
     4261 gacgctttta agatctgagc tcaaattcac tttggcaact ccgtatttta ctgcttcgct
     4321 gacttctgcg tccggattac ctgaaccgcc atgtaaaaca aatggaagat cgatttgttg
     4381 gttcaattct ttcagtaagt cgatatttaa ttttggagtt ctgtttttcg gatacaagcc
     4441 atgcgctgtt ccaattgcta cagcaagtgt atcgattcct gtttctttca caaaaatctt
     4501 cgcttgatca ggatctgtat aaatgattgt ttcagcacct ccttcagcag aaccattatt
     4561 cccaatcgtt ccaagctcag cctccactga gacatttacc gcatgagcta acgcaaccac
     4621 ttttttcgta atctgtacat tgtcgtcgaa tgatttcaaa gacgcatcga tcatcacgga
     4681 agaatagccg tttcgaatcg cccgtaagac atcttctatc gtaccgccat gatccaagtg
     4741 gataacgatc ggcagtttac ttgtataagc ataggctctt actgccgcaa caaactcatc
     4801 acttaaatag gcgatttcat caggatgaat ttctaagatt actggtgctt ctaatctctc
     4861 tgcttcttcg atgattgctt tcatcatatc atagctgcaa atattgaagg ctggtactgc
     4921 aaattgattc tctttagcca ctttcaataa ctctttcatt gtatataaca ttacgcttcc
     4981 acctcatttg ttagattttc atttttcgtc cacttgtctt gttttttctc tttcactgtt
     5041 tttttcaata gtccaagcat aaaggctgta gtgactgtac ctgatgcaat tgccaagaca
     5101 tacataggta aattgttcat aacatttgga attagcatca cccaaatccc tccatgaggt
     5161 gccattgaag taactcctac tgccatggaa atccctgcag cgactgctga acctgtcatc
     5221 aaactaggta tcacgcggat cgggtcagat accgcaaacg gtatagcacc ttctgtaata
     5281 tacgaagcgc ctaaaatcca gcatgattga cctgaagtcc gttcttcttt ggtaaattta
     5341 tgtttgaata ataaagtggc taaagcaata cctaggggag gtgtcattcc tgcagccata
     5401 caagcagcaa tcggtgcatt tactccagct gacatcaaac cgatcgagaa agtcgaagtc
     5461 gatttattga tcggcccacc catatcagct gccatcatga tcccgataag taatccgaac
     5521 aatagcccgg atgtatttcc caagctattc agccaatttg tcaaagcatc caacacccat
     5581 gtgatcggtt gttcaatcac aaataccatc aaaagcccaa taattaagat cgataaaaag
     5641 gggacgacaa tcagctgata aatagcagaa aattcttttt ttacgctaat tttatttgcc
     5701 agaaaatcac agatataccc tgctagcacg ccaccaatca ttccgccaag aaaacctgaa
     5761 ccacctgtag aggccagtaa tccggcaaca attcctgggg caaaacctgc ttttccagca
     5821 atcgatgtag caatccccgc gcctaacgca ggaaccatca tggcaaacgc tgtatctcct
     5881 ccgatccttg aaaatgcagc tgctaacaca ttatagtctg ctgagttggg atcagccgct
     5941 gtaatcccaa aggcaaaact cagtgcgatg atgattccgc ctgcgatcac aaatggaagc
     6001 atgtaattca caccattcat gaaatgatta tagattgtcg gctttttctc atttgcagct
     6061 ggttcttttt cattagacac aacgaatacg ccaatatctc tcaatgcttc gtctattact
     6121 acttgcggtt ctttgattgc tcggcttgta gagacacgct ttactttttt cccggcaaat
     6181 cggtccaatt cgatttcttt atctactgca agaatgattg catctgcctc tttgatttct
     6241 tgttcagata actgattttc tgtttttaca ccttgtgttt cgaattttgc ttgatggttt
     6301 agatttcttg ctgttacttc taatttttct gccgacatat aagtatgagc aattccagtc
     6361 gggcaagcag ttacaccaac gattttcatg attattttcc tcctttttct tcactatcga
     6421 tactcggaca aagactctac tattttcatt cttgactaat ctgtctgatg atatctgctg
     6481 gcgtagtggc ttcttttatt ccctttctaa aatctgcatt catcaatcgt ctggagagtg
     6541 tgctgagaat ctctagatgc atatctgctg atttttcagg aactacgatg gcaaaaatat
     6601 attcaattgg catttcatct atcccgctat agtccgttaa tttggctgta tgaaaaaaac
     6661 acagtgctgg ttttgtcaca tatttgcttt ttgcatgggg aatcccaaat ccatcttcta
     6721 ttcctgtgga gatctcctcc tctcgtttga atagtgcctt aacgactttt tgaacatttc
     6781 ttaccttatt tgtttttttc aacacatccc ctattcgctc aaataatgat ccttttgaat
     6841 ctatcgatgg atcgaaaaca atcagttctt ctgcgatcaa ttgatcaaac ctcataaaaa
     6901 acctctcccc cacaaaaaat gttaacgttt tcttggaact gaggtcagta tagcatcgct
     6961 agctgtaccg ttcaatatta tgaatgaaca gaggaaaaag acccatcatc cgcgtgtata
     7021 agtcattaaa atgaacgatc tagctcacaa acaatcgcaa gtctataaca aaacaaaaag
     7081 ccttccttga aaggaaagct tagcttctaa ttttaggtaa atggtttttt acttcgaaat
     7141 aacccctctt catttcgtta atataaggtg tttttatcaa gtggataatt aaaactatat
     7201 actagttttt cattgttttg tgcattatga tcctacgact ttttcagcat caaacgaagt
     7261 tttaaattgg caaaaaacat aaaaaaacag actatcccta tcagatagtc tgctaaacgt
     7321 tgagttatca gcgtttttat tattccgcag cgtttgcgtc tttgagaccg tattttttgt
     7381 tgaaacggtc cacacgtccg tctgcttgtg tgaatttttg acgtccagtg tagaatggat
     7441 gtgagtcaga agttacttcg acacggatga ctgggtatgt attaccgtct tcccattcaa
     7501 ctgtttcttg tgaagttttt gttgaacctg acaagaattt gaaaccagtt gttgaatcca
     7561 tgaatactac tgggtggtaa tctggatgga tgttttcttt catgattttt tcgctccttt
     7621 gccctgctcc atttgctgaa ccagagttga tttgtcgatt tacaacatta acatcttaac
     7681 atgtttcagt caaggttgca attatctttt cagatttctg gtcggatttt ttttaccaaa
     7741 ggagacatct tgaaattctt tgaaaaattg ctgattgttt tttgttttac gcaaaaagcg
     7801 gatcagttgt tccgtatatt ctaatgaatc tccagtcata ttttttcgaa gtttccaaat
     7861 ttcttctaat tcttcacttg gcatcaataa ttcttctttt cgggtacctg attttttgat
     7921 atcgatggct gggaagatcc gacgttcaga taattcacgt gaaagttgta attctagatt
     7981 tccagttcct ttgaattctt catagatcac gtcatccata cggcttcctg tatcgactaa
     8041 agcggtcgct aaaatcgtca aactgccacc ttcttcgatg tttcttgctg ccccgaaaaa
     8101 tttcttaggt ttatacaatg cggctgggtc aatcccgcca ctcaatgtac gtccgcttgg
     8161 tggaacgact aagttatagg cccgagcaag tctagtaata ctatccatca agataacgac
     8221 atctcgttta tcttcgacta aacgcattgc gcgttccaat actaattcag aaactcgagt
     8281 atggttttgc ggctgcagat caaatgtaga agataccacg tcgcctttca cgcttcgttc
     8341 tagatccgtt acttcttccg gtcgttcatc aatcagtaga acgattaatt ctacttccgg
     8401 atagttttcg gtgataccat ttgcgatttc ttttaataca gaagttttcc ctgcttttgg
     8461 cggtgcgacg atcaatccac gctgaccaaa accgattgga gaaaagatat cgatcattcg
     8521 ggtagatagt ctttgcggtg ttgtttccag ttgaagctgt ctttgcggat aaagaggtgt
     8581 taaggctgga aaatgcggac gttcttttgc ttcttccgga tcttttccat taacgctttc
     8641 cacgtgcatc aaaccataat aacgttccga ttctttaggt ggacgggctt ttcctgccac
     8701 tttgtcccca tttctcaaac tgaatcgtct gatttgtgag gcagaaatat agatatcttc
     8761 tgcacttggt ccataattga ttggccgtaa aaaaccgtaa ccatcttgac caacgatatc
     8821 caatattcct tccatataat aaaatccttg tttctctgct tgtgcacgaa tcacagctag
     8881 agataattcc tttttattca tcttactgta ataaggaatt ttaaattctt tggcataact
     8941 ataaacttct tttaacgttt tgttctctag ttctgccatt gtcaaataat cgctcattca
     9001 gctcccactt cctctatcat ttcaattgat gcccctaagg ctgttagttt ctcgataata
     9061 tgatcataac cgcgtaagat ataatctaca ttgtaaatcg tcgttgtccc ttctgccaat
     9121 agtccagcaa tcactaaaca agcacctgcc cgaagatcag aagccacgac ttctgtgccg
     9181 tgaagtttat ttggaccatt cacgatgatc atatttcctt ccacggtaat atcagcgccc
     9241 attcgtgcta gttccggcac atggttcaca cgtttcgcat agatcgtatc gatgatttct
     9301 gaagtgcctg tagtcatcaa tagtaacgca gtaagtggct gctgcagatc tgtggcaaat
     9361 cctggataag gataggtcat gatgtttgcg ggttttaatt ctttcgaggg gtaaacttcg
     9421 atttcatctt cgctgatttt catatggaca ccaatttcct gcaattttgc tataaagctt
     9481 tccaaatgtt caaagatcac atttttcact ttgataccat ttcctactgc agctgctaat
     9541 gctagatagg ttcctgcttc gatgcggtca ggaatcatga aatgccgaca gccatgtaat
     9601 gtttcgactc cttcgatacg gatcacatct gtacccgctc ctcgaacttt tgcccccata
     9661 ttattcagta aagttgccac atctatgatt tccggttctc gtgcagcgtt ttcaattact
     9721 gtctgtcctt ttgcttttac agcagctaac atcacattga tcgttgctcc gactgaaacc
     9781 acgtccatga aaatccgatt gccacgcaat cctttatttt ctgtccgtaa atacatggca
     9841 ccatgttcat ttgtcacttc agcacctaga gcttcaaatc ctttgacgtg taggtcaatc
     9901 ggtctagggc ctaaataaca gccacctggc aggcctacta ccgcttcgcc aaatttacca
     9961 agcagtgttc ccataaaata ataagaagca cgcaaactat tgatttttcc gtgcggcatc
    10021 ggaatcgata cgacttctcg aggatcgatt tctagtatgt tgttggaaaa atggacagtt
    10081 gcgcccatga tctctaaaat ttcaattaat gaatgaacat cttgtatgtc cggcacacct
    10141 tccaagatca ctggggaatc agctaagatt gcagctggaa tcaatgcgac tgcactattt
    10201 tttgcaccgc tgatcgttac ttcaccagct aaaggtcggt ttcctttgat tacgattttt
    10261 ttcatctgtt tccaccttgt ttattaatct tttttcatgg atatgcttgt attttatcat
    10321 agataaaaaa aagaaacgag catggtctgc cgttttaagt aaaaaagaat ggagagcgac
    10381 ttcaaaagtc gttctccatt cctatatatc gtatctgtta tgtggatgct tacgctttgt
    10441 tagctgaacc gaaccagtcg atatgagatt tagcatcttc aacgattgct gctttacctg
    10501 gagctaataa tttacgaggg tcgaatcctt taccttcttt atctttacca gcttcgatat
    10561 atttacgagt agctgcagca aatgataatt ggaattcagt gttaacattt acttttgaga
    10621 tacccattga gatagctttt tcgatttgat cttgaggaat acctgatccg ccatgtaata
    10681 ctaaaggcat gtctggacct acagcatcag caattgcttg taagtggtca aaagctaatc
    10741 ctttccagtt ttctggatat tgaccatgga tgttaccgat accgcaagct aagtagtcga
    10801 tacctgtagc aaccatttgt ttgcattctt caatatcagc taattcgccg ttaccgatga
    10861 ttccgtcttc ttctccgccg attgaaccaa cttcacattc aactgagata ccttttgcat
    10921 gtgctttttc aacaacgtct ttagctaatt tcaagttttc ttcaaatggt agatgtgaac
    10981 catcgaacat tactgaagta tagcctaatt cgatacattc caaagcagct tcgtaatcac
    11041 catggtctaa gtggatagca actggaacag tgatgttcat tgcttcaact aaatcagtga
    11101 ttaaatcttt acagattttg taaccaccca tgtatttagc tgcacccatt gatgtttgga
    11161 taagtactgg tgcttgtttt gcttcagccg cttcaaggat tgcttgagtc cattccaagt
    11221 tgtttgtgtt gaatccgccg acagcgtaac cgcctttacg agcagctttt aaaaattcag
    11281 ctcctgatac tactggcata ataaaattcc tcctaagttt tgtaaaacta aatttgttaa
    11341 gacaattctt aaccaacgac cctattttac caaacatttc ggtagttttc tactaaaatc
    11401 aatgaaaatg aaaattttca agaagttttt tgcaaaaaat aaaatatcca atagcgcttt
    11461 aatcgaaaaa agtcttttgc tttctatctt ttttcctaca aataataaca aatcagaaaa
    11521 agagaaaagt gacaagaaaa ttgcttatcc ccttctcttg ctacttttct ctttctatca
    11581 agttcaaaaa ttattttgtt tcgtgatctg ctaatgatgc tccgacaaat gctttgatta
    11641 atttttgcgg gcgatttgga cgagaaatca attctgggtg gaattggcat cccacaaaga
    11701 attttttctc tggcagctct acgatttcta ctaaacggtt atctggtgag acacctgaaa
    11761 agactagccc ttgttcttca aacaattgac gatatttgtt attgaactca tagcggtgac
    11821 ggtgacgttc ttgcacaaca tcggctccat catatgcttc agccgtcttc gttccttttt
    11881 tgattttaca tgggtataat ccaaggcgta aagttccacc caaattctcg atgttttcct
    11941 gatcagccat taaatcaata atattgtttg ctgtatctgg attcgtttca gctgatgacg
    12001 catcttctaa tccaacgacg ttacgtgcaa attcgacaca agccatctgc ataccaagac
    12061 aaatacctaa aaacggaacg tcattttcgc gagcataacg aatcgcttcg attttccctt
    12121 ctactccgcg atcaccaaat ccaccaggaa ccaagattcc atctgcgtct ttcaaacgtt
    12181 cttctacatt ttctctcgtt aattcttgtg ctttgatcca atcgatctcg atgtctgaat
    12241 tataggcgaa accagaatgt ttcaatgctt caactactga gatatatgca tctggtaact
    12301 cgacatattt accgacaagc gcaatccgta cttttttctt caagttcaag acacgttctt
    12361 ctaattcttt ccattctgtc atgtctgctt ctggtgtatc tagttttaaa tggtcgcaaa
    12421 caatttgatc cattccttgt gcttgtaaaa ctaatgggat ggagtaaagt gtatctacat
    12481 ctggcgactc gataactgct tctggttcga catcacaaaa ttgtgccaat ttgtttttca
    12541 cgctttgaga aacaggttgc tctgtacgta ccaccaaaat attcggttgg atacctaaac
    12601 cacgtagctc ttttacactg tgctgcgttg gtttagtttt catttctcca gcagctttca
    12661 agtaaggaat caatgttgta tggatataca taacattatc tgtaccgaca tctgctttca
    12721 tttgacgaag tgcttcaagg aatggcaaag attctatatc ccctactgtt ccccctactt
    12781 ctgtaatgat cacatctgaa tcagtcattt tagctgctcg catgattttt tctttgatct
    12841 catttgtaat atgaggaata acttgaacag ttgctcccaa gtattcgccc ttacgttctt
    12901 tacgcaaaac ttcagaatag atttttcctg tcgtcacgtt ggagtatttg ttcaaattga
    12961 tgtcaataaa acgttcgtag tggcccagat ccaaatctgt ttcagcacca tcatctgtta
    13021 cgaatacttc tccgtgctga tatgggctca tcgtccccgg atccacgttg atgtatggat
    13081 caaatttttg gatagttact tttaagccgc ggtttttcaa taagcgtcct aaagaagctg
    13141 caacaatccc ttttccgatt gatgaaacga caccgccggt cacaaaaata tattttgtca
    13201 taagttcaaa aactcctttt taatttatgt gcaggaaaag tctcagacaa taaacaaagc
    13261 tccctattct tcaataagaa tagggagccg aaatttctgt aaacttctga ccctctaaaa
    13321 gggtgcccaa ggtgtataat acaagctgac taagggaaag tcaagtactc aaatgaatac
    13381 tatccgctta caattttttc tttttcgata tcctttgaga aattatttct taagtccttt
    13441 atgagtgcaa aaaaaattta acccacataa taagcttgat ataaacatag ctaacgttaa
    13501 acctaagcaa atcagaaaat cgttactgaa aaattttaag tgaaataata gataaaaaaa
    13561 taaaacaaac attatgatag ataatatccc acctaacaaa gaactacttt ttcctacttg
    13621 cagcaacagt ttatatagct tatcgtagaa acaatctttt tctttcaccc ttatttacct
    13681 cctttacgta aaaaacgtag tccacagttc tcggattttt ggcatatctt ttctattaaa
    13741 taatatcaaa aacaaaacaa ttccactaat tttttcaaag atgacctttt tttcaacaaa
    13801 agtgccttgt agggattcat tgaaggtaac gaatcacata agtaaaaaat aaggagatgc
    13861 gacaaaagtc gtgcctcctt atttctaaat aaacggtgtt ctcctgccaa ctcctcggtc
    13921 acaagtcaca atatccatca aacatgagaa gctgtttttg aaattatttc ttgtgattcg
    13981 gagttaaatg gtaggctcac aacctctgga taaacggt
//
LOCUS       DACECJ010000074        13918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.074, whole genome shotgun sequence.
ACCESSION   DACECJ010000074 DACECJ010000000
VERSION     DACECJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.074"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWO09_002215"
     CDS             131..505
                     /locus_tag="IWO09_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0264796.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWO09_002216"
     CDS             complement(619..1248)
                     /locus_tag="IWO09_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0264797.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWO09_002217"
     tRNA            complement(1382..1454)
                     /locus_tag="IWO09_002217"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IWO09_002218"
     tRNA            1668..1741
                     /locus_tag="IWO09_002218"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IWO09_002219"
     CDS             1696..1845
                     /locus_tag="IWO09_002219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264798.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IWO09_002220"
     CDS             2338..3666
                     /locus_tag="IWO09_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0264799.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IWO09_002221"
     CDS             3868..4701
                     /locus_tag="IWO09_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0264800.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IWO09_002222"
     CDS             4717..5454
                     /locus_tag="IWO09_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0264801.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6535)
                     /locus_tag="IWO09_002223"
     CDS             complement(5609..6535)
                     /locus_tag="IWO09_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0264802.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="IWO09_002224"
     CDS             6810..7643
                     /locus_tag="IWO09_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0264803.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="IWO09_002225"
     CDS             7677..8516
                     /locus_tag="IWO09_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0264804.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="IWO09_002226"
     CDS             8542..9102
                     /locus_tag="IWO09_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0264805.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="IWO09_002227"
     CDS             9301..11022
                     /locus_tag="IWO09_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0264806.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11187..12329
                     /locus_tag="IWO09_002228"
     CDS             11187..12329
                     /locus_tag="IWO09_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0264807.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12345..13556
                     /gene="thiI"
                     /locus_tag="IWO09_002229"
     CDS             12345..13556
                     /gene="thiI"
                     /locus_tag="IWO09_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0264808.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4036 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
    11041 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
    11101 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
    11161 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
    11221 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
    11281 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
    11341 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
    11401 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
    11461 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
    11521 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
    11581 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
    11641 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    11701 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    11761 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    11821 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    11881 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    11941 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    12001 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    12061 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    12121 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    12181 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    12241 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    12301 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    12361 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    12421 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    12481 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    12541 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    12601 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    12661 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    12721 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    12781 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    12841 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    12901 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    12961 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    13021 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    13081 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    13141 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    13201 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    13261 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    13321 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    13381 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    13441 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    13501 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    13561 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    13621 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    13681 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    13741 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    13801 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    13861 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DACECJ010000075        13701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.075, whole genome shotgun sequence.
ACCESSION   DACECJ010000075 DACECJ010000000
VERSION     DACECJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.075"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IWO09_002230"
     CDS             complement(<1..902)
                     /locus_tag="IWO09_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0264809.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IWO09_002231"
     CDS             1185..2153
                     /locus_tag="IWO09_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0264810.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IWO09_002232"
     CDS             2266..2544
                     /locus_tag="IWO09_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0264811.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IWO09_002233"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IWO09_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0264812.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IWO09_002234"
     CDS             3010..3756
                     /locus_tag="IWO09_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0264813.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IWO09_002235"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IWO09_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0264814.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IWO09_002236"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IWO09_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0264815.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IWO09_002237"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IWO09_002237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0264816.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IWO09_002238"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IWO09_002238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0264817.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IWO09_002239"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IWO09_002239"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0264818.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IWO09_002240"
     CDS             8493..9035
                     /locus_tag="IWO09_002240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0264819.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IWO09_002241"
     CDS             9058..10614
                     /locus_tag="IWO09_002241"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0264820.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IWO09_002242"
     CDS             10628..11530
                     /locus_tag="IWO09_002242"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0264821.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IWO09_002243"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IWO09_002243"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0264822.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IWO09_002244"
     CDS             12973..13395
                     /locus_tag="IWO09_002244"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0264823.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2451 c         2988 g         3732 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag a
//
LOCUS       DACECJ010000076        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.076, whole genome shotgun sequence.
ACCESSION   DACECJ010000076 DACECJ010000000
VERSION     DACECJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.076"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWO09_002245"
     CDS             complement(<1..914)
                     /locus_tag="IWO09_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0264824.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWO09_002246"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWO09_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0264825.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWO09_002247"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWO09_002247"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0264826.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWO09_002248"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWO09_002248"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0264827.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWO09_002249"
     CDS             4440..5321
                     /locus_tag="IWO09_002249"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0264828.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWO09_002250"
     CDS             5318..6034
                     /locus_tag="IWO09_002250"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0264829.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWO09_002251"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWO09_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0264830.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWO09_002252"
     CDS             6751..7752
                     /locus_tag="IWO09_002252"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0264831.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWO09_002253"
     CDS             7749..9110
                     /locus_tag="IWO09_002253"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0264832.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWO09_002254"
     CDS             9070..10221
                     /locus_tag="IWO09_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0264833.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWO09_002255"
     CDS             10196..11365
                     /locus_tag="IWO09_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0264834.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWO09_002256"
     CDS             11540..12919
                     /locus_tag="IWO09_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0264835.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWO09_002257"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWO09_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACECJ010000077        13404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.077, whole genome shotgun sequence.
ACCESSION   DACECJ010000077 DACECJ010000000
VERSION     DACECJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.077"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            79..1125
                     /locus_tag="IWO09_002258"
     CDS             79..1125
                     /locus_tag="IWO09_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0264836.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IWO09_002259"
     CDS             1474..2106
                     /locus_tag="IWO09_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0264837.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IWO09_002260"
     CDS             2694..4133
                     /locus_tag="IWO09_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0264838.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IWO09_002261"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IWO09_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IWO09_002262"
     CDS             4895..5110
                     /locus_tag="IWO09_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264839.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IWO09_002263"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IWO09_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IWO09_002264"
     CDS             complement(5770..6681)
                     /locus_tag="IWO09_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0264840.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IWO09_002265"
     CDS             complement(6700..7704)
                     /locus_tag="IWO09_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0264841.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IWO09_002266"
     CDS             complement(7701..9824)
                     /locus_tag="IWO09_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0264842.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IWO09_002267"
     CDS             complement(9829..12276)
                     /locus_tag="IWO09_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0264843.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IWO09_002268"
     CDS             complement(12263..12652)
                     /locus_tag="IWO09_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0264844.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IWO09_002269"
     CDS             complement(12712..13215)
                     /locus_tag="IWO09_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0264845.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..>13404)
                     /locus_tag="IWO09_002270"
     CDS             complement(13228..>13404)
                     /locus_tag="IWO09_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264846.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4099 a         2642 c         2454 g         4209 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DACECJ010000078        13399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.078, whole genome shotgun sequence.
ACCESSION   DACECJ010000078 DACECJ010000000
VERSION     DACECJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.078"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(15..707)
                     /locus_tag="IWO09_002271"
     CDS             complement(15..707)
                     /locus_tag="IWO09_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0264847.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            792..968
                     /locus_tag="IWO09_002272"
                     /pseudo
     CDS             792..968
                     /locus_tag="IWO09_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="IWO09_002273"
     CDS             complement(1144..1713)
                     /locus_tag="IWO09_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0264848.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="IWO09_002274"
     CDS             complement(1787..2110)
                     /locus_tag="IWO09_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264849.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="IWO09_002275"
     CDS             complement(2264..3076)
                     /locus_tag="IWO09_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264850.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="IWO09_002276"
     CDS             3430..4278
                     /locus_tag="IWO09_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0264851.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="IWO09_002277"
     CDS             4423..5364
                     /locus_tag="IWO09_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0264852.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="IWO09_002278"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="IWO09_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="IWO09_002279"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="IWO09_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0264853.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="IWO09_002280"
     CDS             complement(9828..12014)
                     /locus_tag="IWO09_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0264854.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="IWO09_002281"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="IWO09_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0264855.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="IWO09_002282"
     CDS             complement(12236..12676)
                     /locus_tag="IWO09_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0264856.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="IWO09_002283"
     CDS             complement(12750..13289)
                     /locus_tag="IWO09_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0264857.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DACECJ010000079        13323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.079, whole genome shotgun sequence.
ACCESSION   DACECJ010000079 DACECJ010000000
VERSION     DACECJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.079"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            509..715
                     /locus_tag="IWO09_002284"
     CDS             509..715
                     /locus_tag="IWO09_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0264858.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(917..1918)
                     /locus_tag="IWO09_002285"
     CDS             complement(917..1918)
                     /locus_tag="IWO09_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0264859.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1923..3836)
                     /locus_tag="IWO09_002286"
     CDS             complement(1923..3836)
                     /locus_tag="IWO09_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0264860.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(4004..4510)
                     /locus_tag="IWO09_002287"
     CDS             complement(4004..4510)
                     /locus_tag="IWO09_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0264861.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4670..5110
                     /locus_tag="IWO09_002288"
     CDS             4670..5110
                     /locus_tag="IWO09_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0264862.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5269..6609
                     /locus_tag="IWO09_002289"
     CDS             5269..6609
                     /locus_tag="IWO09_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0264863.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6678..7835)
                     /locus_tag="IWO09_002290"
     CDS             complement(6678..7835)
                     /locus_tag="IWO09_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264864.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7838..8194)
                     /locus_tag="IWO09_002291"
     CDS             complement(7838..8194)
                     /locus_tag="IWO09_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264865.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8492..9412)
                     /gene="sgrA"
                     /locus_tag="IWO09_002292"
     CDS             complement(8492..9412)
                     /gene="sgrA"
                     /locus_tag="IWO09_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0264866.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9608..10447)
                     /locus_tag="IWO09_002293"
     CDS             complement(9608..10447)
                     /locus_tag="IWO09_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0264867.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10632..11519
                     /locus_tag="IWO09_002294"
     CDS             10632..11519
                     /locus_tag="IWO09_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0264868.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(12103..12798)
                     /locus_tag="IWO09_002295"
     CDS             complement(12103..12798)
                     /locus_tag="IWO09_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0264869.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12782..13180)
                     /locus_tag="IWO09_002296"
     CDS             complement(12782..13180)
                     /locus_tag="IWO09_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0264870.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         4003 a         2653 c         2064 g         4603 t
ORIGIN      
        1 tggagaaggt tgtattggta ttattcgagt tacttgttag atgcgataac aaagttagta
       61 tacagtagta aaaaatttat cttttctttt ttttattatt tttgtttctt attttctaaa
      121 aaattaaaaa aaattttggt gaattaagta tttccttgat aacactggct tttggcctca
      181 ttttaggtta tcatttgtaa ttttatttca cagttgatat gattgttatg taaaataaaa
      241 aagtttagga tacatcgaat ttttggggaa attcttttgg gggtatccta aatcttttct
      301 attatattcc catattttct attattggtc tagacgtatg atggtaaact ttgtacctat
      361 ctgattgctg aatcattaat tgcttatcat tagccatctt tgtttcaagt tcgctagatg
      421 atcattttat tcacatccta acaaacttgt cacatttata gattcttaat ctatttatca
      481 tacaattata aaaaaggagg tgctgcttat gaaagctatc catatttctt tgcttttttt
      541 cgctagtgca ttgattttat ttgctattca tctttgttcc cagccttata tcaataacaa
      601 cggaatatta gtggaaccag cattcttttg cttaccactc ggctttttgt cccttcttgc
      661 ctccgcaggc ttcggatttg ttgctttttt taaacgatca aaacaacaaa tctgacttat
      721 aaaatttacg taaaagagat cgtgacaata atcgtgtgat attcccaaat ttcgaataaa
      781 aggtgcccct tgccatctct aactcctcag tcacaagtta caatattcat aaaccatgaa
      841 aagcttgttt attgaaattg ctccttgtga cttggagcta agtgacagat tacaacctct
      901 tttgcatcat aagactttac tccttgaaaa acattactaa cttttgatat gtttcttgcg
      961 cttgtttatt gctgaaatca aactgatatt catggttcaa aacagcattt ttctccgcat
     1021 aatctacttt tgtgactttc acccccgctt tctcaaatac agaagttact tcttccattt
     1081 gtttcgtaaa cgtatttgta tttccatccg ttaagaatgt gttttgcgtc caacgatccg
     1141 catgtctgac caaggctaaa cttttgacct tctctgattg ttcaaagtcc ttattatcaa
     1201 agtaagccca tccacatttt tcataaagaa agctagagat tttatcactt acttgagtaa
     1261 gtgcagaagg atccaacaga ccagacaaag aaataaactt cgtaatctgc ttgttctcca
     1321 aagtcggatc aagatgatca gcttttttaa tttcttcatt ttgaagtgct aacaaatatt
     1381 ctccactaat ttgtgcacct gccgaatttc caccaatcga aatattcgac caatctgctt
     1441 gatcttttat ctctttttta gctgcttgaa tggcttcgtt caattggata actggtgtag
     1501 gatgttttgc ttccggtgat cgttcataat ctatatttac gatgatttgc tgcgtgtctg
     1561 aacatagata ttccaaataa tcacgacaat catttttatc ccctcctaca tatgcgcctc
     1621 catgcatcca gaaaagaatc ggtaattttt tcttttccac tttcggtgtg tagatatcca
     1681 tattattatt tttgtacttt gaaggatagg atatttcttt ttttatatcg atttgttcga
     1741 ttttttgctg gaaatcatct ggtcgggcat atacttgttc tttttcactt gtgtcaaatt
     1801 gttttctaac aagcaagcta atcggctttg gtgtgatttg attaatcaaa aatacagtga
     1861 ctaaaataac tacgatgata actggtactg caatcactat tcgcttgaac catttcattc
     1921 atttaccctc tctttcttcg ccaaatccat gtgataatcg tgctaacaga taaaaatcca
     1981 cctgaataaa tgagaagcga atgtttcttt tctccagttt tcggcaacat tctttttttt
     2041 gcatctgtct ttttttgtac tttcactgtc atctgaatcg gttgcgtatc atcacttgat
     2101 tgatcctctc gaggttcatt ctttccctta ttgtctgcat tctctgtcgg atcttgcttt
     2161 tttggtggag ctactgggat ttttttgtaa tagaaaataa tagttggtat cgtctcatta
     2221 aaaatccctt cagtcggtcc ttcaacattt gctaattcgt atccttcgaa tgtcttttgc
     2281 tctgtcatat aattttctcc ataagctccg taaagctgga tttcgtcctg gtctttgagt
     2341 agacttttat tgttttcatc cacatacttt acgtttatct gatcatggaa cactactttc
     2401 aaaaagtccg ttacatgttt cgtgtaggca gcatggtcaa cttcctgtgc ctttccatgc
     2461 cctgctcctt ctactaataa aagatcttta taccctcctg ataactcata taatttttgc
     2521 gcgttagaca caggtactac atcatctgct gttccatgga taatcaattt aggcaatgaa
     2581 tctgacattc caatcgtagg tagagcagct tttgtatcca aatgcatctc ctgatcaaaa
     2641 tagtattgtt tcaaaaggcc cataaataaa tcttcatgct cttgatcccc aatgatgtct
     2701 aactgatttt ttatggcatc cggtagtgcg ggggctactg tctggctgta cgtaaaacgt
     2761 agttgttcat caattgatgc aaatccgcaa ttttcaataa ttcctttgac attgacagga
     2821 atatctttcg ctaatacaga catcatcgta gctgctccca tcgaaccacc ataaagtaaa
     2881 atcttttgag atggccgctc tcgaacttct tgatttatcc agtaaaggac gtcatctgac
     2941 tcatattgtc cataggtcac ataatcgcct ccgctattac cagtcgctct ggaatcaaca
     3001 aataataaat tatacccggc cttataaaaa tcattgtatt cttctgtaac gatcccgtta
     3061 ttccaattcc ctcgaaatcc gccatggatc atgactgttt tatctgaacc ttgatcgaca
     3121 taatatgcat atacatccat cattttgcca tcttgtttgt ttagtacagt ttgatgaagt
     3181 tgttttgtat ttggatagaa gcctgtgttt tctgtaggcg ttgtcccatc atctgtacct
     3241 ccaagcatcg acatcaacac actgtttttt gcaaaagcga tatcgtacaa tagataagta
     3301 gcaatcagat gtttgataat ctctttatct acaccataat cattaacagc aagctcagtg
     3361 actaataact ctattaattt tgacttgatg tcttctgttg gtccattaag tatttctgtc
     3421 atctgtttac cgaagtaatt caaatattct ttaccattga aattttcacc tttaccgata
     3481 tcatcatttt ctgtcccatt ctctttgtca cttactttag cagaagagga ttcgtcgttt
     3541 gacatttttg tagctgttgc ttcttctgtt tgttggttat ctgtggtagc ggtcgtttct
     3601 tgatctgtgc tagtaacaga tgtctcaacc gttgtttcag tatttgtttc tgtttgtgaa
     3661 gtactcgatt tcgtttcatt tccagcatca gtagtttctg tggtttctac ctcccgagat
     3721 tccgattcgg aagaaatgct ctctccatat actgtccatt gcgggacgct tgtacttaca
     3781 gcaaataaga tgaccaacgc tccctttaat acttgttttg ttctcaagct attcactcct
     3841 ttttatgttt ctaggagaat tatatgtaaa cgcaaacatt ttttatacaa aaaataaatg
     3901 gtttatccca taaaaataga acaggattat tttctcattt tacgtaataa agcagaatgg
     3961 ataaaccagt atccattctg ctttttttaa acgaagaaac aacttattgc tccaaaaatg
     4021 tcagaaatcg tttatcccaa attttctcgt aaaaagcgat tgtttccttt gctttcataa
     4081 aaggattatc ataagttgtc gttgctcctc tacacatcat aagctggtat cctaatccat
     4141 gagcaaactt cacagtcgta tctatacaat actctgcttg tgccccacat aattcgatcg
     4201 aatgacagtc caattcagtt aataaaacac tcaaattagt cttataaaat gaattcgcat
     4261 gggtttttct cacaaaataa ctgtgctcag gaatagataa ttccgacaat agctcccatg
     4321 gttttgtttg tggcactaat tgagcatctt cgtgttgaac gaaaataact ggtttcccaa
     4381 tcgtttcgta tgttttgatt cttttattca ctaatgctgt cactgtttct aattcgtgta
     4441 tcggcgaaga atcaaaacat accccttttt gtaaatcgat cactactatt gcgtcggctg
     4501 tgtttgacat acttgtgcct tctttctttt ttcttgattt aatagtattt cttatctagc
     4561 tagttccatt atacgtgaca acattttctt attttacacc tctaaatggc acttttcgac
     4621 tatttcttca tgttattatt tcataaatac ttagctattg gaggaatgta tgatgattaa
     4681 attgtataca ggttctggct gctcgtcttc aagacgcgca aaacgttggc ttatcgtcca
     4741 tgatataccg tttatcgaac agaacttaat tcgtcaacca ctaaccaaaa atgaatttat
     4801 ccacttacta aaattgacaa acaacggcgt gaccgaaatg ctctctactc attctattag
     4861 ttataaaaat cttgttcaca gaatagatga actcagtttg agtgaactat atattgagat
     4921 tcgtacatcc ccttctttat tgaaaaaacc gattatctac gaccaatctg caaaactttt
     4981 gactggattt agcgaagata aaatcaggat gttcctttct aaaactaaaa gaaagcaaga
     5041 gctcaacgaa ctcttgcttt ctggtcatct tgtcgatttt aaagaaaata atctttttgc
     5101 aatgtcttaa ttttggcata aacaatctgc ttttctggat ctataatatc tagtgttggt
     5161 gacgccttct gttgccaata ctttttcttt tttctaataa acgcaaacag acaccttgaa
     5221 atcctttaga ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat gtcctattca
     5281 gagtttacca aagagttact agatattcta gacctaaatc ttacctttca tgaagatgcc
     5341 tttcgcaaag agcgcattaa tgacgaaaca tgttttgttt ttgatggcac attgacctat
     5401 caaccagagg aatgcttcca ttgccactat caaaataagc aaacgatcat taaatggggc
     5461 tggaaaaaag tatccatctt attgaatgat gtcagtaact acaagaccat tgtgcggatt
     5521 aacaaacagc gcttcaaatg taaacactgt ggaaagactt ttttagcaga agattcagtt
     5581 agcgatcgtc gctgctccat tgcccgcaga gtcaaacaag ccattcttga gctattaagt
     5641 gagcctattt ctatgtcttt gattgcacgt atgaagcaca tttctccgac cactgtcatt
     5701 cgaatacttc gcagccttcg gcctaaaacc gtttctttga accagccgtt accagaagtg
     5761 gtgtgtttcg atgaattcaa atcggtgaaa aacgtctcgg gagccatgag ctttgtcatg
     5821 atggacggca agacccatca actgattgat attgtggaaa atcggcaact caatccttta
     5881 cgcgactatt tcttgcgcta ccctagaaaa gtccgtgaac aagtacgtct agtggtttca
     5941 gatttctata caccctatcg cacattggtg aaagagtgtt ttccgaatgc ccgaatcgtc
     6001 gccgatcgct ttcatattag ccaacacatc ggtcgggcct ttaccaacca ccgaattcaa
     6061 gtaatgaaga cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa atactggaga
     6121 ctacttcaaa agaatgcgtg ggaattgaat ggtcagcatc gctactggcg tccgtcattt
     6181 cgagatcatc tgacagaagc ggagatagtc gatcggctat tatattatga tgactcctta
     6241 aagcgaggct acgaagtcta ccaggttttt ctttccgcta tcagaagaca agatgttcct
     6301 gaattcgtcg cgctcctaaa agaagactac aaggagttgc cagagcacta tcaaccagtg
     6361 ttcacgacct tcaagaaata tcgaacagaa atcaaacgag cgttgcgagt tccctattcc
     6421 aacggcccta tagaatgttt gaacaaccat atcaaagtac taaaacgaat tgcctatggc
     6481 tttagaaact ttcaaaacta ccgagaacga atctttttat atcgaggaaa atatttcaag
     6541 aaaactaaga acactaccca actgactaaa gccagaacaa caacacggct agacaagata
     6601 gctgtctagc cgtgtgagta tctcgattat tctattgaat ttcttcacca acactcattg
     6661 acgaagagcc gcttttctta attaacgaaa tccaattttt ccaatatgga tctatttttc
     6721 tcattcctga tttttatgat ttcagctttc agacgcgccc aatcattttt tgtaggttgt
     6781 ccattccagt aaaatatctg tgtattcgtt tgatcatatg cctttttgtc gataggataa
     6841 tcgctaatga caatatcagg taactgatca gcggatgcac tatattttat tgggcgttca
     6901 gtcatagata atatcctttt ctttgcccat aattcctgtg ttttaccata ctttgattga
     6961 atatagaata tgacaggttg ttcgtgtttt ataaaaatat ccctgactaa catacagtat
     7021 tgaaaaatca accgtctgtt cttttcataa taagtattaa gctctggaat tctgcgagct
     7081 accccactaa aaaactgttg catgattgga tacatcactg gaaatatatg ctgaaaatct
     7141 ctgtcttctg ctgattttcc aaaagcatcc accttctttt ttgtcccaaa aacccttgaa
     7201 tacaaatgga tcattgccaa attgacatat agataatttt tttctgaagt tgtaaaacaa
     7261 atcttgaagt ttttttcaaa attttctaca atagacgatt caatccagcc ccaactttct
     7321 tgtactttat ctggagtatc caacaaatct tcttccaaat aggtgttcat tacaccaaac
     7381 ataaaataaa ggtaagcttg ctctgttgga gtgatgtgat tcatcgacca gaagtttgct
     7441 tcaaaaaaag gctctagtat gtaatgcgta aactcttctt ttgttatcaa tggatgcaca
     7501 aatttgttca tctcttctgg gacatacgta atcgagtggc cttgtttcat tcgttgcaac
     7561 gaaattctta gtaggctgat ccatttcttt ctatctatac tacgcgtata tggcgcatgt
     7621 ttcgcaaatt tttctaatag ttgacgttta ttttttatac ttgataatat atcagaatga
     7681 ttttcgtcga atgactgcca atacatcaag tgaaaaaagt atcggatacg gtattcttct
     7741 ccttctatcg gattaccttt tttcaagctg caagataaat gatagttttt caaaatagta
     7801 ttaagttctc ttaattttct ttgaatcgta cgcaaactta tataattttt tgactaagat
     7861 tttctaagga ttgataagta cctttgtaaa gtgaatccag taaaatataa ccaatcgttc
     7921 cctctaaaag tatagaataa actttactca gtaaaaactg agaagaaaac tcgactttta
     7981 tcatcttgtt ttctgaaaga aaagataatt tatattctgg atacctttcg caaaacgatt
     8041 ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta gtttagatct aaagcgatat
     8101 ccttcaccac atataatttc tcgtctcttt cataacaata gttcaaaaat tgatatactt
     8161 gcctttcatt gatatcaata aattcttcaa acaaagtaca tactcctttt tatctattat
     8221 ttagacttat tccgacagct acatgaataa cgtacaaata gattcgttac tgatttttca
     8281 gagatactac catgcgcagt gtgcattgtc aattattctt gctcttattt tccatttgtg
     8341 ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa aaatagatct ggcaaagcta
     8401 caacttcgcc aggcaataca gcactctcga atgaaatatt cactcaaaaa tctatcgttt
     8461 tattcaattt tagatctgtt gcttttattc tttatttcac tcgatggaag agagaacgaa
     8521 aatagcttgc tcctacaatc aaaatgagta acacacccat ccatgtaatt agcttgttca
     8581 aggttctact accagtttct ggtaaatcgc tttttctatt atctgtttct ttcgcttttt
     8641 cttgatgatc aattccacga gaaacaaaat caggctgttt tagcacactt tttaggttgc
     8701 tccctgcatt tctcttttgt tccttagttg gtatgatagg taccaaatca agttcattat
     8761 ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt gctactctca ctactcatgc
     8821 ttcctgtgct actttcacta ctcgtactct ccgtactgct ttcgctgctc gtgttttctg
     8881 tgctactttc actgctcgta ctctctgtac tgctttcacc gctcgtgttt tctgtactgc
     8941 tttcactact tgtgctatcc gtactgcttg gcggtccaac aactgtccaa ctgcttggtt
     9001 tcaaattctc cgtgatcgtc atctgtccat cataaaaaac aatcaaatca actttatctt
     9061 tactcttgat ctcatcaatc gatagttctg cagaaatcat tattgtatac tcaataccag
     9121 cggtccattg tacgtctttt aaataaactt ccccaataac tttttctgaa cttccattac
     9181 catcccaacc atatgttaga acagcatgta catcactagc attcacgctt ttgtttggtt
     9241 tgactggaat agtaatgact tgttgtgtca tgtcataaac aatccgatca gttttcacag
     9301 taaatgaaag attctcattt gcccgttcat ttgcagaagt actgattcca atcattgctg
     9361 caattatact cagcccaaga ataacaaata atacggtcgc tgttttcttc atttctatca
     9421 ctctccatat aattaccttc tatttgataa aggaattata taaaatgaaa atgatgagaa
     9481 cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg tattcgcttg aatttgttta
     9541 ctttttcgtt taatcaatga cagacttaaa aaagcaagac cgatatcagc cttgctttct
     9601 tcatctttta ttgataaaca gcagtgtatt tcttcatccg ctttgtttta tatgcataga
     9661 gtaacaaacc aacaatcaag actaacaaga ataatgtagt tgatatagat gcttcagccc
     9721 caaagtttcc cccagtaatt attgtatttt gcgattggat cagagtggag aaaaaacctt
     9781 gttcttgcgt tccgctgact ggtataccca aaatagctcc ttgtgtccaa ttccaagtaa
     9841 tatgagcacc aatagccgta aataaacttc ctgtactgta gataagtgcg ctgaaaaagc
     9901 aacctgctaa aaagatattg aaaactgcca gaaatgtcat gttaggattc aatccgtgca
     9961 aaagagcaaa taatgcagca gatccaatca ttgctattaa cggaccaaat ctaagcatct
    10021 gatgctgaaa aaagccacgg aataaaagct cttctgatgc ccccactgca ataaataaaa
    10081 taaaactcgt tatcagcggc acaatgtttg cgtttcctga atctaaccga tacgcccccg
    10141 tcaaatataa tggactagta ataagcagaa acactccaat tccaacaacg agacctcccg
    10201 ctaaataccc ccagtctttt cctcgaacag aaacaccata atcttctatt ctttttcctt
    10261 cgaccttact agcaagaaat aaagctagta aaaaaacagc taaatgtccg accgtaccgg
    10321 caattgctcc aatccattca ctatttccaa taaacatatc cgcaagtgca ttattgacaa
    10381 gaattcctaa aacaaaacca actaagacta ttaaaacaga tccaatcaac cgaataatcc
    10441 tgatcatctt tcatccctcc tttatattaa gtttaacaac ccttttcttt cttgtctcgt
    10501 gaaaactatg taaatgttct ccatcataga gagaatagat ttttacgaac taagaatatc
    10561 tacagcaacc ttacgaaaat gaaagactgt ctccctaatt cttctgttag actgttttta
    10621 ataaggaaag gatgggaaat tttttgaaaa aacaactact aattatgtgc ctttcagcat
    10681 gtgcaatttt actattctca ggttgtagca agtccaatga aacgattgca acgtttaaag
    10741 gtggaacact tacagttcag gatttttatg ataacccaaa agttaaatca aataatcaaa
    10801 cactattaaa aaaaatgatt gtcttcaaag catttgatga tgtatacgga aaagagattt
    10861 cttcaaagga agtaacaaaa gaatataatg aacaaatcaa aaaactcggt ccaaactaca
    10921 aggaacaatt aaaagcagtt ggccaaacag aagagacata taagaaactc tttaaacaga
    10981 tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt aacagataaa gatctagaca
    11041 ctgcttggaa agaattctat ccagaagtta gtacacagat cattctattt tcgactaaag
    11101 aagaagcaga taaagcaaaa aaagaagcaa atgaagggga aaatttctcc aagcttgttc
    11161 aagcttatgg aaaaaataaa acattaaagg aaactgatgg aaaaatgaat tttgattcaa
    11221 ccaatccaga gattcctact gaagtaaaaa aagcagcatt taaactaaaa aacggggaag
    11281 tttctgatat cattcctgta acagatccaa cgacttatca gcaaagctat tatctagtaa
    11341 aaatggtaaa aaaacaggat aaaggttcta ataaagacaa atacaaatct gagctagaaa
    11401 aaattgctac cgaagcaaaa actcttgata cggaattcat ggataaaaca atccgtaaag
    11461 tgatgaaaaa agacaatgta actattaaag atccttatgt aaaaaatata ttcaaataaa
    11521 caaatttgac ccaattgggg atgtggcaaa atttgagaag tgatttttgg aaattccttc
    11581 ttatttttga ggcaaaccac ttttgccaca acttcatcgg ggcctttttt tatttgtacc
    11641 aaaacaaatc actggatttg ttacaatcat gaggatataa tttaaaaaag ttttttattt
    11701 ctatcacatg aatcaatgcg ccattaagta gattctttca tgcttcccta ttttttcact
    11761 tgccgtatcg cttcaaaaac aactatcata aactcaaact cgactgaaaa gttattttaa
    11821 aaaagactaa aaatatccct tctggacttg ttcctcttat tctgttatac tagagaaaca
    11881 cgaaagatat ttttatgcga cacttttcat ttgtttttat gaaatttatt caaaactggc
    11941 gtaacttcag agttacaagg atgtattaga ggttagggaa tacatcgttt aaatgagaaa
    12001 taagtaatag tcacccaaac tttaaattaa tgaatattga atcgtcagat agcctcctcg
    12061 agttttcgag aaggctatcg tttattttat ggagaaattg attcagaagt aatcagcgac
    12121 cttttttagt tcaccaatcg tttctgcttg gaaagtttgc acccaatctt cttggaagcc
    12181 cctcactgct ttatcaatcg agggcatctc taatacttga tctagtagct ctttacctaa
    12241 cgttatcgca tgagctccag atcgaatggc tgtagtgact tgttcacaat ttttgaaact
    12301 agctgctaat atttttgttg gtctttgcgt acgttcgatc tcagtccgca acgaatcaat
    12361 cactttcata gcatcaatgc cttgattttg catcctgtta taataaggtg caatataatc
    12421 tgctccatta ttaatagcta aatacccttg taattctgtg taaatagctg taccggtaat
    12481 caaataacct tcttttttca actttttgat cgcttgaagg cctgcttgat tcaccggcac
    12541 cttgataaac gtatcttttc ccagttcttc tatgataact cgagcatcct ctatcatttc
    12601 ttcttccgtt cttcctattg tctgaatatg gataggaatc tgtccgatta tttttttgat
    12661 ttctttcata tgcgagaaga agtttgacgc tccttctttt ttcagaattg taggatttgt
    12721 tgttacgcct gttaaattca aaaactgttg acaagaataa atactttttg tatttgctgt
    12781 atcaagcatg atttccatat agctttcccc cattctgatc ctcttgatcg attttaatga
    12841 ttgtcccctt tcttaaatgc ggcatttcct tatcttctac atacattgtt cctggaagtt
    12901 ccggctcatg agatccatca aattttatcg tgatatgtcc taattcattc aaattagttt
    12961 ggacaacatc tcctactgct gtgattacat acatttgttt atcaaaacag agcttcatat
    13021 cacaagttat ttcttttctt acctgattca attcaatctt ataacaataa tctgttaatg
    13081 tttccggtgc ttcatctcca aataaaatca ttattttttc tttcctgaat acttctgctt
    13141 ctataccaac atctgttaca accgtttcaa agatcgtcat ctctgttcct cctcgtaaaa
    13201 tatcatttgt ctaatagttt ttattcaaaa tgtttctaga ttataaaatt tattgaataa
    13261 cacattccta acggaatttg tttcgatatt gtgtgatttc tcagagtaca atggatgtat
    13321 gga
//
LOCUS       DACECJ010000080        13222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.080, whole genome shotgun sequence.
ACCESSION   DACECJ010000080 DACECJ010000000
VERSION     DACECJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.080"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            158..1027
                     /locus_tag="IWO09_002297"
     CDS             158..1027
                     /locus_tag="IWO09_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0264871.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1236..2261
                     /locus_tag="IWO09_002298"
     CDS             1236..2261
                     /locus_tag="IWO09_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0264872.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2300..3250
                     /gene="manA"
                     /locus_tag="IWO09_002299"
     CDS             2300..3250
                     /gene="manA"
                     /locus_tag="IWO09_002299"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0264873.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3344..5047
                     /locus_tag="IWO09_002300"
     CDS             3344..5047
                     /locus_tag="IWO09_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0264874.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5049..7082
                     /locus_tag="IWO09_002301"
     CDS             5049..7082
                     /locus_tag="IWO09_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0264875.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7093..7533
                     /locus_tag="IWO09_002302"
     CDS             7093..7533
                     /locus_tag="IWO09_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0264876.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7733..8860)
                     /locus_tag="IWO09_002303"
     CDS             complement(7733..8860)
                     /locus_tag="IWO09_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0264877.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8863..9540)
                     /locus_tag="IWO09_002304"
     CDS             complement(8863..9540)
                     /locus_tag="IWO09_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0264878.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9804..11570
                     /locus_tag="IWO09_002305"
     CDS             9804..11570
                     /locus_tag="IWO09_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0264879.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11585..12022
                     /locus_tag="IWO09_002306"
     CDS             11585..12022
                     /locus_tag="IWO09_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0264880.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12035..13186
                     /locus_tag="IWO09_002307"
     CDS             12035..13186
                     /locus_tag="IWO09_002307"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0264881.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4491 a         2298 c         2731 g         3702 t
ORIGIN      
        1 cacaacctct ttttataaga gtttttactc attaatttac aaaaaacaag caaaatgcga
       61 attttctcac atttttttta acaaaatcaa agttattgct tgaaagaaga agaatccatc
      121 gttaaaataa agcttgacgt agaggggagc aagaaatatg aaagattttc aattgggggc
      181 tgccacctat ttaaaaagtg tagccattcg ggtgaaagac cgagataaaa tgattgattt
      241 ttacaagaac gttgttggct ttgatttgaa aagagaagaa aacgaattag ccattttagg
      301 aacagaagaa gctggaagtg aaatgttgct gctggaagaa agtcctagag cgaatgatca
      361 catgggggtg atcaaaaagc tgaaccgttt ttcattgatt atccctagca cagaagaaat
      421 ggcagatatc ctttgtcgta tccgtaaaat gaattatcca atcaaagaag ctttagaaga
      481 tcatggacgt atggggattt tgctcgtaga cccagaagag aatgaattgg aaatttacca
      541 tgaagagaat aagaacgacc aaagagaaat tccagaaaca atggatcaag aagcattatt
      601 aagaaaatct gaagaaaaat tcagtaaact atcaacaggt tcttattttg aaaaaatcca
      661 tttaaatgtt actaacttaa caaaagaacg ccaatttctt aatcatgtac ttggtttgaa
      721 ggtacataat gaagaagagg gcatccatgt attgaatgaa ggagatttcc atgttggttt
      781 gacagaagct agcggaggcg caattgattt gcctacagat gaagtgattg gacttgattt
      841 tctgaaattc atgatcgata ctgaaagcat ggaaggactg attgctcact tgcagcaact
      901 atcagttgat ttcttcttag acaaaaagca cacagtactc acaatctatg atgctatagg
      961 aatcgaatgg tggttcgtca gtgagccaaa agaaaagaat aaaactgaca ttcgatgcga
     1021 cgcttaactt tttctttctt aacgattgtc gcatgtctcg tcattttatg aacggtcata
     1081 ctatcgctta ttctagagat agaaagagaa aatagtatac cgggggaaac tgcgcggttt
     1141 ttcgcgcagt ttttttgtgt aaaaaaggtt gattcctcat agattaagct ttaagcttgg
     1201 actgtaagaa tctgatagaa aagcaggaga aaaagatgta tccacacacg aaacagctta
     1261 ttttgaaaaa gatggaagaa ggattttatc caggtgtagc agtcgccttc ataaaagaaa
     1321 atcaagtaga aacatttaca gccggctttg cccaagtcgt tcctgaaaag cagctgatga
     1381 cagaagatct gttgtttgat gcagcctcac tgacgaaagt cgttgcaacc acttcattag
     1441 ttttacgtct attagaagat ggaaagatag aactcgatca accgcttcat aaatatcttc
     1501 ctgaatttga aaatcagaca atcactttgc gtcatttact gacacatact tctgatatca
     1561 aaacatggat accaaacaga gataagctat ctcaggaaga attgatcgcc gcttatttga
     1621 agctagaacc aggtgagcaa ctaggaaaag aagtaaaata tacagatgcc ggtacgattc
     1681 ttcttgggtt gatgctagag gaaatttacc aagaaactgc agtggaaatc tttcaagaac
     1741 aagtattgga accattagga atgatgaaca gcttatttct tccttttcct tccaaaaaaa
     1801 tcgttcccac agaacaactg ccttcgggtg aggttttgcg tggacaaact catgatccga
     1861 aagcaagagt tttggctgaa catgcaggta acgcgggtct ctttattgat ttgaaagaca
     1921 gcattacttt tgtccaaatg tatttgaaca agggaaaaat ccgtaatggt cgttttttag
     1981 aagaagagac gattgattct ttactgcaag atcaaacacc gaaaaaaaat ggctatcgtt
     2041 ctttaggatg ggacttaaaa aaggatgtag aagacggtca tgcgattctt tttcatactg
     2101 gttatacagg tacgtttttc ttgattgata taaaaacaca agaagcgttg atatttttgt
     2161 caaatcgcgt acatcctgtc gatcatcgtg cggagtatct catatgtcga gatgaaattg
     2221 ttgaaaactt tttgaaagag aaagcggtat taccgaaata atgctataat agctatgaaa
     2281 aatgagagga gtttgaatta tgcaggaacc tatgttttta aaaccagttt ttcaagaaaa
     2341 aatttgggga ggaagtcgtt tacgttcagt ctttggtttt gatatcccta acgataaaat
     2401 cggagaagat tgggcgatca gtgcgcatcc tcatggtgtc agtgtagtag aaaatggcga
     2461 attcaaaggg aaaagattag atgaactatg gtcagaacac aaggaattgt ttggccatcc
     2521 ttcagaacca gtgtttccat tattgatcaa gatcttagac gcagaagatg atctatctgt
     2581 acaagtacat ccagatgatg cctatgggat gaaacatgaa ggtgaattag gaaaaactga
     2641 atgctggtat atcatcgatg ctgaacctgg tgcagaaatc atctatggtc atcatgcaaa
     2701 gacaagagaa gaactagctg agatgatcaa agatgggcgc tgggatgacc ttttgaagaa
     2761 agtaccagtt aaaaaaggtg atttctttta tgtaccaagc ggaacgatcc atgctattgg
     2821 taaaggaatc atgattttag aaacacagca aagttctgac acgacttacc gcgtgtatga
     2881 ctacgacaga aaagatgcgc aaggacaaac aagagagctg catatccaac aatcgatcga
     2941 tgtaacaact gtccctgcga aaacaccaga attacaaatc aaagaagtcc gaaaagggaa
     3001 ttcatcgatc gtcacttatt tagaaactga gtttttcaat gtatatgagt gggatattaa
     3061 aggaatcaca tctttcaaaa aacaagcccc ttatacattg atgacggtaa tcgaaggagc
     3121 aggagacctt gtcgttgacg gaaaaacata tccattagaa aaaggaacaa gctgtattat
     3181 tccaaatggc gtttctgagt gggcggtcca aggtgaacta gcaatcattg cgactgaacc
     3241 aggcaaatag ttcccagata gctgaaagca gcagacggtt tttcgtttgc tgctttttct
     3301 acataaaatc cgtccatcaa taaatagaag tatggtgaaa aagatggaat acaaatggaa
     3361 agcaaacaac agcagtcgcg tgtctatgaa acaactgaga aaagctggac atcacttaaa
     3421 tgaaatgatc atgccaaatc taagtctgtt tatcgcttgg ggaatcttgt cattagcaac
     3481 aaaaggtgtg tctggcgatt tgcacaccac attagaagag gtatgccatt ggatgatcca
     3541 gctgctttta cccatcttga tcagttattc aggcgggaaa tcggtagggg gacaaaaagg
     3601 ggcagtcgcc ggtgcaattg cttctttagg gttgatcgct cgttccaatg ctccacaaat
     3661 cgtaggagcg atgattatcg ggccgcttgc cggcatcgta tatgaacaat ttgtttctag
     3721 attccacgtg aaattcaagg ctggatatga aatgcttgtc agcaacctac tagtaggact
     3781 gactggtagc gttatttgta taattggaat cgtttttatt gaaccagttt taaatagtgt
     3841 tcacttgttg ctcgcctccg ttgtgtcttg gctagtcaca atgaatctgt taccacttgt
     3901 cagtctgatc ttagaacctc taaaagtact atttttcaat aatgcaatca atcatggtgt
     3961 attgacacct ttaggaatcg aattctcaca aacagtcggg tactcttcct tatttttgat
     4021 ggaggcaaat cctggacctg gtttagggat cttactttct atcctttgtt ttgcagaaaa
     4081 acaggaaaaa gtaaatgcga gtggagcatt gatgatccat gcgattggcg ggattcacga
     4141 aatctatttt ccatttgtct tgctgcgacc gtatctattt ttagctgtaa tggctggtgg
     4201 ggcaagcggc acgttgatat ttcaatggtt agatacggga ttagctgcac ctgtatcgcc
     4261 tgggtcacta ctcgtgattt ttgccaatgc gccttatcca caactagctg gggtaagtat
     4321 agggatagca atcagtacat ttgtgacctt catttgtgca gcgatactat tgaaattacc
     4381 aaataaagaa aaagagaaca aaacggtcaa agaaagggga gaaatcttgg aaccatctat
     4441 cattcattcc gtcatttttg cttgcgactc aggtatggga tcaagtgcta tgggagccgc
     4501 cctgctgaaa aagaaattag cagcagaaaa aatcgatctt tctgtcggat acagctctat
     4561 ctatgaattg aataaccaag taaatcagct ggttatcgtg caacatgaac tagcgggtct
     4621 tgcaagagaa aaagtacctc aaagtcaaat ctatgtactg gatcactttt tggaaacaga
     4681 tatcttgctg tcacgaataa aagaagcgat ccaatttcca aagcgggaag aaaaaaaaga
     4741 gatagcagat atcacagaac aaaaaaagtc gcctgctaat attgtatttt tgtataaaaa
     4801 caacagacgc ggctcacaaa ctatgggcat gacgatttta agtcaattag caaaaaaagc
     4861 gaaaaaacag tttgttatct caaaagaatc aatagaaaaa atcaaaatcc aagaagaaac
     4921 gatttatatc gcacaaagag aactagctgt aactgaacag ctagaagaaa agatccctcg
     4981 gctagttcta gttgaacatt gggtagctac gaatgaatac gaaaaactcg tgaagggaga
     5041 aatataagat gtatttatcg cccagagaaa aacaattatt agcagaattt ctcagtagcc
     5101 cgtcacctgt atctatccaa aaaatgatga atttattgaa ggtcagtaaa cggactattt
     5161 accgcgaatt agataatctt gaagtctcat tgaaatcggt aaatgcttct ttgaaaaaag
     5221 cagctagagg ctcatttgtc attgaggcat cagaagaagt attagagcaa ttaaaagaaa
     5281 atacagatgg gaaaagtaat gacttatcca ctgctaaacg acaacacgcg attttagccg
     5341 agttgttact gacgacagag ccgttgagtc ttgctcattt catggaatca tatcgcatca
     5401 gtaataccac tttttatgca gatatcaaac agttggaaga aagtattcgc caactgcctt
     5461 tggaaatcat tcggaaccag ggatacgaga tcgaaggacc ggaaaaatac cgccggcttc
     5521 tgacagcgaa tgtattagaa ttagaaatca atgaatatga actttttcat agtatttctt
     5581 tcgatgcatc actgaattat ttttttcgat tcgttgatcc gcaacatcta tctcttgcac
     5641 gcaaagtaat cggggaagaa ttgatccaaa aaaagaccaa tctaagtgat agaaagcttg
     5701 aacatttggt tttgatgctg acattgacga tggagcgtgt gactaaaaat catttactga
     5761 caaatgagac atataatagt ctggcaaaca aagaattact acagacagct aaacgattgt
     5821 tttcacggat cgcttctgag acaaaacaac tttatccagt aaatgagatc gtcttttttg
     5881 caggattatt aaatgacttc acgaactcat ttgatcagaa tttctttgaa gagacgttcg
     5941 atacacagct tgcttattta gtaaaacaac tgattcaaga agtaagtgaa gagacgaatg
     6001 tccattttta tgaagatgaa actttgtaca aaatgctgtt gactcattta tctggtgtgt
     6061 tttcaagagc cgttttacaa gaagagacac tgaccaatcc gattttagaa aaaatcatgg
     6121 ttcaatacga ggaagttgcg aatgcaatcc gtaaagcagt agagcggatt tttcaagaaa
     6181 aaaaattatc cgaagaagag atcgcttata tggtccttca ttttgcgaat tcattggaac
     6241 gaagtccgaa agaaagcaat atcagtattg caggattttc gccaagtgga ttagcttcta
     6301 ctagcatgtt ggaactacga ttgaaaaggt attttccctt catccaacaa attcatttct
     6361 atcggattgc tgatttaaaa aaaatcgatt tacaagaaga atatgatgtt gttatttcta
     6421 catctatttt accaggatac ggtggaaaat atattttagt ctccccgcta ctgctagaag
     6481 atgaagtcaa gcagctaaaa gaagcttttt cgcatattga gaagaaaaaa aggcaaaaaa
     6541 ttacgtataa cagcaataac gagtggatga tagaagatac ttatgaggaa acaatgcatt
     6601 tcatggatca aatcaatcaa ttactcggtc gtttttttat ccaagaactg gataatcctg
     6661 agacgatttc cgaaacgatt cgcagcttat ttccttattt tcctgaagga ctagtaacgg
     6721 atgaagaggt ggttcatcgc cggctgctta agcgatatga acaagcacct atcggtattc
     6781 ctcatacaaa tatggggtta ttccatacat ccagctcatt gatttctaaa ccgatctttt
     6841 gtattttcaa tttgaaacat ccgttgacca ttgaagggat ggatagacag ccagtcgatt
     6901 tgtcccgtat gttagttatg ctggcgcctt cgccaatcca agaaaaagac gggattcttt
     6961 taggaaagat cagtggagca atcatcatga atgacttgaa caccgagatc ttccgctcag
     7021 gaaataaaga aatcgtttat cagttgttag cgaaaatcct gattgaagaa ataaagaaat
     7081 gaaggtgaaa agatgataca gctaccagca gaaaaaatat tgctcgatca atcctatcaa
     7141 aattggaaag aagcgttgac cgagatcggc cagtggatga cccaacaagg gggaatcaaa
     7201 ccggcatata tccaatcaat gatcgaaaga caagaaaaag cgagtgtcta tatcgggaat
     7261 ttcgtagcct tgccacatgg gactgacaaa gatgaagaag tagtagaaga aggaatctat
     7321 ttagtccaag tgcctgatgg tgtggacttt ggaacaaaag aggaaccaaa aatcgcgaca
     7381 ctcttatttg tagtggcttt aaaaaaagaa catcagttag cggtgctcca agatttagcc
     7441 tttttctgtt cagacatcga ccaagtaatg gcgctcagtg atgcgaaaac cattgaagaa
     7501 gtccaaatga tttttgaaca tggacatata taaccaatgt tcacagtaaa aaaagctgcc
     7561 ttgcatcaag taataacttc aaccaagata aaaataagag gagctatttt tgttgggagg
     7621 ttcttattac tgcggatgca agcttttgaa aactatttat tctagaaaac cgtttatttg
     7681 aaatagggga cggtgacaag gtttttgtca ccgcccctat tatttttgtg ttttaatagc
     7741 tcttttttat atagatagtg cccataccta tgaataataa aacgatactc catataccaa
     7801 gtgtcaagat accattcatg aaagtaacct gttctgaagc tagtggcgag aaaatatctc
     7861 ctcctatgat cacattttga aaatcaacat aagaagttgg caagtaatga ctgattgatt
     7921 ctatccatcc aatcgaagaa aacaagcgat actctgctaa gaaaagacaa agaagtgtac
     7981 ctaaaagatt gactagagaa tgttcaaata aagcagatag aagaaaactg attgtactta
     8041 aaaagagcac ccacaacaac aggaacagaa agctctggaa aagatactca tacatcggaa
     8101 ggattcgtat ctcctgacca atgatcgttc ctacaggata acggaagtct cccaaaccat
     8161 ttttgattat tactagtata gagacgatca aaaatggcat cacgagactt agcatagctg
     8221 caccataata aatggtaaaa cggatgctat gtttctttac catactatcc ggtaatagat
     8281 tgactagaga gattacctgc tttctttttt cataagagat gatggaagaa agaaacaagg
     8341 cggttataca taaaatcaaa aaactagaaa tcattcctga aaaaatattt tcgtagtaat
     8401 ttgctaaagg gagatgcgct ggcaagtcaa aaatagaata tctttggatc tgatgttctt
     8461 tcatataacg gagcaactcg acacgttttt gttgttcaat gattggaatt ccttgtcttt
     8521 gaccagaaat cagtcgatcc agtatatctt tttcataatg ataagttgct tcgaccattt
     8581 gctggacatt ccctgtgttt tttgcatgta agattgcttc gatcagtttg ttgcttttct
     8641 ctaaactttc cacaaattct ttctcattct catcttcttt caacgtttca atcaaacgtt
     8701 gcatctcttc atagttatga ttcagttcat cgaattctgt ataagcaagt ttatgatcaa
     8761 aaaaaagtgg gaatgcaaag agcacgacca acaatagagg aaataaaaag tttactttgt
     8821 tttttagtaa gagattgcgt tcaaatgtaa gtaaagacat agttacacct catataattt
     8881 acgataaata gctaaaatct cttccatatt tgaggattct gtgacgattt ctccattttt
     8941 tagaaaacat gctttagaac aaatcgattg gatattcgcg atagcgtggg aagaaagaag
     9001 aatcgtcttc ccctctttct gccattgtct gatgaggcta ttcaagatat caatacttcc
     9061 tgggtctaat ccgttcaacg gttcatcgaa taacatgaca gatgaatcac tgatcaagta
     9121 agcagcaagc aagacatgtt gtttcattcc tagagacatt tttccaatcg gcaaagtaag
     9181 gtactcagtc atcttcaatc gttggaaaac agtttggcat tctactttgc tctgccaata
     9241 gtatttgatc aaagtcaaat aatcaacagc cgatagatag tcggataact gattcgttga
     9301 ttctagaaaa aagaaagacc ggcgataatc tgtataagca gtaggagaga tgttgtgaag
     9361 ggacaattga tcataagttg ttggcagcag ttgggcaatg ccattcagca aagttgtttt
     9421 gccaaatccg ttcggggcaa tcaaagcagc tacttctcct gattctacta aaagattcaa
     9481 ttgtcgaatc acacgttttt taccacgttt catgttcatg tttttgatca gtaatgtcat
     9541 agataggcct ccaatataaa atttaataac ataatagaat ggaaaacatc taaattaaat
     9601 gctaacaaaa gttattgttt gcatattata aaaaaattaa aataacagtt ttatttaaaa
     9661 ttgaagtttt ctgataccga aatcttttta ttttgctgac atactcaaac ctttggcaca
     9721 aagtttcgtg ccaaagtacg actatttctc tttttagtaa gcggatacaa ttagagtatc
     9781 aaaaggagag agggagagac aaaatggaac ggacgattcc caaaaacaac atttcgctaa
     9841 aagcgagggt acaaaaatta ggcagtactt tatcaagcat ggtcatgcca aatattggag
     9901 cactgattgc ctggggtgta ttaacggctc tatttatccc agatggttat ttaccaaatg
     9961 aggctttggc taccatggta agcccaatgc taacttatct gattccttta ttgatcggtt
    10021 atactggcgg aaaagtaatt gctggtgacc gcggatcagt tgttggggca attgcaacga
    10081 tgggtgtgat tgtcggaaca gatattccaa tgatgctagg cgcgatgatc atgggacctc
    10141 ttggtggata tgccatcaag aaatttgatc agctattcca aaaacggatc aaatccggtt
    10201 ttgaaatgct agtcaataat ttttcagcag gtcttatcgg ttttggatta gcgttattag
    10261 gtttttctgc gattggtcca gttgtcgatg cattgactca agcaatggca aaaggtgtag
    10321 agatcatttt atctgcccat ttgattccat tgacaagtat ttttattgaa ccagcaaaaa
    10381 tcttattttt gaataatgca atcaatcatg ggattttgac accattggga actgaacaag
    10441 tagcagaggc tggaaaatct attctattct tgcttgaagc aaatcctggt ccaggacttg
    10501 gcgtattact tgcttttact ttgtttggca aaggagcagc aaaatcttca gcaccaggag
    10561 ccatgatcat tcacttttta ggcgggatcc atgaaatcta ttttccttac gttatgatga
    10621 aaccgttatt attcttatca gttataggcg gaggaatgtc aggaagtttc attttccaat
    10681 tgatggatgc tggactaaga gcgccagctt ctccagggtc gatcattgcg attctagcga
    10741 tgacaccgtt caattcttac cttcctgtta ttttgggcgt gattgttgcc gcagctgttt
    10801 cgtttggtat ttctgcagta attttgaaag cggatgcgaa agatacaaat gaagacttcg
    10861 aaaaatctgt tcaagagaca aaggcagcta agcaagcttc caaaggtgcc caaggtattc
    10921 ctcaaggagc tacattctct ggcgtacaaa aaatcatttt tgcttgtgat gcaggaatgg
    10981 gatcaagtgc gatgggcgct tcgatcttga gaaaaaaagt acaggaagca gacttgcctc
    11041 aaacggtgac caatcaagca atcagccaac tgacagatga ctcgaataca ttgatcgtta
    11101 ctcaggtcga actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt tttgtctcag
    11161 tagaaaactt tttgaattca cctcgatatg atgaaatcat tcaagaatta agcagcaagc
    11221 agaaaacatc agtttctgaa attgagaatg agccagcaga agagtttctt tcagcaaaca
    11281 tcaaacaaat caaagagatt ctacttgtcc acgatgatcg ttccggatca gcaacaatgg
    11341 ggatgaccgt gctcaaagat atattagaca agaaccattt aactatcccg ttaagaaaag
    11401 tcaatatcca agatttaaaa gatgaacctg attcactgat cgtagccaaa gaaagtttag
    11461 cggaaaaagt acagatggct gcacctcaat ccattcattt atcagtcaac agcatggtca
    11521 atccgcaaaa atacgaatcg attgcacaat atttgaaaca gacagcttaa gggaggaaaa
    11581 cagcatgcaa agtttgccat tcgacatgat cgagctcaat cgatctttta aaaccaaaga
    11641 agaagcgatc cgctattgcg gtcagatgct tgtagaagca gggtgcgttg atgaggaata
    11701 tatcgatgcc atggtagata gagacaaaat gttatctgtt tatatgggga attttatcgc
    11761 gattcctcat ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca tctgtgtcgt
    11821 tcaagtacca gatggtgtag attttggtac cgaagaagaa gaaaagattg cgactgtttt
    11881 gttcggtatt gcaggagtcg gagatgagca tttgcaactt gtacagcaaa tcgcacttta
    11941 ttgcagtgat atggacaatg ttgtccagct agcagacgcg ctcagcaaag aagaagtaac
    12001 ggaaaatcta gcaattgctt aaggagagag aaaaatgaga gcagtacatt ttggtgcagg
    12061 aaatatcggt cgaggattta ttggagaggt tttggcagct aatggatttg agattacttt
    12121 cgtagatgtg aatcacacga tcattgatgc tttaaatgaa agaaaagaat acgaaatcga
    12181 attagcagat gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca
    12241 aacaaatcca gaaaaagtaa tccaagaaat cgctgaagca gatttagtta ctaccgccat
    12301 tggcccgaat atcttacctt ttattgctga gttgattgca aaaggtatcc aagaaagaga
    12361 aaaggaagga agcacaacat ctttagatat cattgcctgt gaaaatatga ttggcggttc
    12421 agcatttcta gaaaaagaag tgtataaata tcttccggaa acatcattta ctgacaaata
    12481 tatcggcttt ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc atgaagatcc
    12541 tttattcgtc caagtggaac ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa
    12601 taaaaaaatc caattaaaag gggttttata tgtagatgat ctagaaccat atatcgaacg
    12661 caaactattt agtgtcaata ctggtcatgc gactgtcgct tatacaggtg ccttgttagg
    12721 ttaccagacc attgatgaag caatgcaaga tgcgttagtt gtggcacaat taaaatcagt
    12781 tttgcaggaa acaggaagtt tgctgattgc caaatggggg tttgatgcag agcagcatca
    12841 tgcgtatatt gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac
    12901 acgtgtcgct cggacaccat tacgtaagtt aggttatcaa gaaagattta cacgtccagt
    12961 gagagaacta caagaacata acttgacttg cccacatttg acggcaacaa tggggattat
    13021 ttttaattac tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca
    13081 tgaaaacttg gaacaattga ttcaagaagt cacaggaatc aatgatccga aaacaatcgg
    13141 gaatatcaag caaaacgtga accgttatgc caagcaagtc gcttaaaata ggatacgaaa
    13201 aaggggaatg tgacgaaatt tt
//
LOCUS       DACECJ010000081        13003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.081, whole genome shotgun sequence.
ACCESSION   DACECJ010000081 DACECJ010000000
VERSION     DACECJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.081"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IWO09_002308"
     CDS             173..1309
                     /locus_tag="IWO09_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0264882.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IWO09_002309"
     CDS             1446..1790
                     /locus_tag="IWO09_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0264883.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="IWO09_002310"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="IWO09_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0264884.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="IWO09_002311"
     CDS             complement(3485..4084)
                     /locus_tag="IWO09_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0264885.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="IWO09_002312"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="IWO09_002312"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0264886.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="IWO09_002313"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="IWO09_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0264887.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="IWO09_002314"
     CDS             7290..8408
                     /locus_tag="IWO09_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0264888.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="IWO09_002315"
     CDS             complement(8468..9760)
                     /locus_tag="IWO09_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0264889.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="IWO09_002316"
     CDS             9910..10470
                     /locus_tag="IWO09_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0264890.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="IWO09_002317"
     CDS             10599..10976
                     /locus_tag="IWO09_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0264891.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="IWO09_002318"
     CDS             10909..12981
                     /locus_tag="IWO09_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0264892.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         4238 a         2327 c         2801 g         3637 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tga
//
LOCUS       DACECJ010000082        12900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.082, whole genome shotgun sequence.
ACCESSION   DACECJ010000082 DACECJ010000000
VERSION     DACECJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.082"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(260..979)
                     /locus_tag="IWO09_002319"
     CDS             complement(260..979)
                     /locus_tag="IWO09_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0264893.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="IWO09_002320"
     CDS             complement(1028..2674)
                     /locus_tag="IWO09_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0264894.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="IWO09_002321"
     CDS             2853..3443
                     /locus_tag="IWO09_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0264895.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="IWO09_002322"
     CDS             3535..5040
                     /locus_tag="IWO09_002322"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0264896.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IWO09_002323"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IWO09_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0264897.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="IWO09_002324"
     CDS             complement(6476..6994)
                     /locus_tag="IWO09_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264898.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="IWO09_002325"
     CDS             complement(7010..7336)
                     /locus_tag="IWO09_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0264899.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="IWO09_002326"
     CDS             complement(7349..9328)
                     /locus_tag="IWO09_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0264900.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="IWO09_002327"
     CDS             complement(9349..9663)
                     /locus_tag="IWO09_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0264901.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="IWO09_002328"
     CDS             9831..10124
                     /locus_tag="IWO09_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0264902.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="IWO09_002329"
     CDS             complement(10202..11314)
                     /locus_tag="IWO09_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0264903.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11701..11880
                     /locus_tag="IWO09_002330"
     CDS             11701..11880
                     /locus_tag="IWO09_002330"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264904.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="IWO09_002331"
     CDS             12074..12244
                     /locus_tag="IWO09_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0264905.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12900)
                     /locus_tag="IWO09_002332"
     CDS             complement(12668..>12900)
                     /locus_tag="IWO09_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0264906.1"
                     /translation="TPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLP
                     KTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4075 a         2588 c         2191 g         4046 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ctattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
//
LOCUS       DACECJ010000083        12759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.083, whole genome shotgun sequence.
ACCESSION   DACECJ010000083 DACECJ010000000
VERSION     DACECJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.083"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(9..530)
                     /locus_tag="IWO09_002333"
     CDS             complement(9..530)
                     /locus_tag="IWO09_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0264907.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IWO09_002334"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IWO09_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0264908.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IWO09_002335"
     CDS             4610..5497
                     /locus_tag="IWO09_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0264909.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IWO09_002336"
     CDS             5494..6663
                     /locus_tag="IWO09_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264910.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IWO09_002337"
     CDS             6687..8042
                     /locus_tag="IWO09_002337"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0264911.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IWO09_002338"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IWO09_002338"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0264912.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="IWO09_002339"
     CDS             10328..10618
                     /locus_tag="IWO09_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264913.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="IWO09_002340"
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="IWO09_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0264914.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11659..12318
                     /locus_tag="IWO09_002341"
     CDS             11659..12318
                     /locus_tag="IWO09_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0264915.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..>12759
                     /locus_tag="IWO09_002342"
     CDS             12475..>12759
                     /locus_tag="IWO09_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264916.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLY"
BASE COUNT         4040 a         2339 c         2815 g         3565 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag
    10801 tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatat
//
LOCUS       DACECJ010000084        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.084, whole genome shotgun sequence.
ACCESSION   DACECJ010000084 DACECJ010000000
VERSION     DACECJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.084"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO09_002343"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO09_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0264917.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWO09_002344"
     CDS             complement(322..891)
                     /locus_tag="IWO09_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0264918.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWO09_002345"
     CDS             1077..1979
                     /locus_tag="IWO09_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0264919.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO09_002346"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO09_002346"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0264920.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO09_002347"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO09_002347"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0264921.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO09_002348"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO09_002348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0264922.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWO09_002349"
     CDS             8547..8732
                     /locus_tag="IWO09_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0264923.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWO09_002350"
     CDS             complement(8794..9990)
                     /locus_tag="IWO09_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0264924.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWO09_002351"
     CDS             complement(10160..11596)
                     /locus_tag="IWO09_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0264925.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWO09_002352"
     CDS             11889..12698
                     /locus_tag="IWO09_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0264926.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2675 c         2249 g         4007 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaacgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACECJ010000085        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.085, whole genome shotgun sequence.
ACCESSION   DACECJ010000085 DACECJ010000000
VERSION     DACECJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.085"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO09_002353"
     CDS             6..305
                     /locus_tag="IWO09_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0264927.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO09_002354"
     CDS             378..1739
                     /locus_tag="IWO09_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0264928.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO09_002355"
     CDS             1761..2021
                     /locus_tag="IWO09_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264929.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO09_002356"
     CDS             2035..2787
                     /locus_tag="IWO09_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0264930.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO09_002357"
     CDS             complement(2881..3486)
                     /locus_tag="IWO09_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0264931.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO09_002358"
     CDS             complement(3539..4513)
                     /locus_tag="IWO09_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0264932.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO09_002359"
     CDS             complement(4891..5670)
                     /locus_tag="IWO09_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0264933.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO09_002360"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO09_002360"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0264934.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO09_002361"
     CDS             7161..8558
                     /locus_tag="IWO09_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0264935.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO09_002362"
     CDS             8639..9079
                     /locus_tag="IWO09_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0264936.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWO09_002363"
     CDS             9227..9553
                     /locus_tag="IWO09_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0264937.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWO09_002364"
     CDS             9554..10303
                     /locus_tag="IWO09_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264938.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWO09_002365"
     CDS             complement(10383..10838)
                     /locus_tag="IWO09_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0264939.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWO09_002366"
     CDS             complement(11265..11540)
                     /locus_tag="IWO09_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264940.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWO09_002367"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWO09_002367"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0264941.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACECJ010000086        11745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.086, whole genome shotgun sequence.
ACCESSION   DACECJ010000086 DACECJ010000000
VERSION     DACECJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.086"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..300)
                     /locus_tag="IWO09_002368"
     CDS             complement(<1..300)
                     /locus_tag="IWO09_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HAQ0264942.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFL"
     gene            complement(521..1387)
                     /locus_tag="IWO09_002369"
     CDS             complement(521..1387)
                     /locus_tag="IWO09_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0264943.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            1496..2137
                     /locus_tag="IWO09_002370"
     CDS             1496..2137
                     /locus_tag="IWO09_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0264944.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(2199..3560)
                     /gene="mgtE"
                     /locus_tag="IWO09_002371"
     CDS             complement(2199..3560)
                     /gene="mgtE"
                     /locus_tag="IWO09_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0264945.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(3578..4477)
                     /locus_tag="IWO09_002372"
     CDS             complement(3578..4477)
                     /locus_tag="IWO09_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0264946.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(4479..5276)
                     /locus_tag="IWO09_002373"
     CDS             complement(4479..5276)
                     /locus_tag="IWO09_002373"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0264947.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(5254..5958)
                     /locus_tag="IWO09_002374"
     CDS             complement(5254..5958)
                     /locus_tag="IWO09_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0264948.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            6089..6664
                     /locus_tag="IWO09_002375"
     CDS             6089..6664
                     /locus_tag="IWO09_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0264949.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            6782..7459
                     /locus_tag="IWO09_002376"
     CDS             6782..7459
                     /locus_tag="IWO09_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0264950.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(7538..9346)
                     /gene="pepF"
                     /locus_tag="IWO09_002377"
     CDS             complement(7538..9346)
                     /gene="pepF"
                     /locus_tag="IWO09_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0264951.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSGSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(9522..10520)
                     /locus_tag="IWO09_002378"
     CDS             complement(9522..10520)
                     /locus_tag="IWO09_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0264952.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(10746..11399)
                     /locus_tag="IWO09_002379"
     CDS             complement(10746..11399)
                     /locus_tag="IWO09_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0264953.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         3483 a         2502 c         2007 g         3753 t
ORIGIN      
        1 taaaaagaag tgctcttctc catcagcaaa attttcgtca ctatatactt tatgggcaaa
       61 ttttgagatc aaatgtagtt tacgattacc taaaaatgtt aaaaaagctt ctaaattact
      121 gtggtaagta atatttacat cattccaata atccaaccct gcccgtttta gatgtttgtc
      181 atcagttgaa aaacctagtg gctcgattaa atgaagcgga gtattcgtgg ctgcacacgt
      241 acgggcaata tttccagtgt ttgcagggat taatggttca aataaaacga tatgattcat
      301 caggatcatt cctctcatat ttatgttcgt ttctattttc tgacaatttg atttttgcat
      361 aaaaaaaaac gtatcaactc ggtgtcagtc actgcgagtc gttacgttag tgaagctctt
      421 ctcactaact tttatcagaa caggaacctg ataaaaacca atgaaaaaac aacttgttgc
      481 taatataaca ccctcaactt ttttttgcaa cctctactca ttaaaaactg ctagaaaaac
      541 gttttctctt agttcctttt aataattcta tgtcaattgt aatcttcttc aaaggattct
      601 tatataagtc tgctttgatt cgctcttcta cctgccattc taaaggtgac atggccaaaa
      661 gatctttttg attttcctct ggtatctcaa attcataggt tacacgctga cgttcagtta
      721 atttcattgc ttcagcaaac ttggctacta cacgttcatt ggaataactc tgtttttcag
      781 gttgattcgg ataaaaagcc tgacgtagtt cctttaaata attttcttgt ggcaccacct
      841 tcaacacttc tccatcttct gagagtacac gttggaattc ttggtaatga gaaggcgaaa
      901 agatatttaa aaccgcgttg aatgtttgat cagcaaacgg caaattggtc aaatcagcta
      961 cacaccaaaa tgcttcggca gattgttctg tagctgcgta gactccttct tttgaaatat
     1021 caaatccaac agcattttga atctttttca attttgaaag ttcattcaag aagctacctt
     1081 ctccgcagcc aacatctaat agcgagactt cttcaggtaa atgctgcgca atagtctcaa
     1141 taacgggctg atacatgcca gattggatca ttctacgcct tggaacgaac attccttgat
     1201 cgtattctgt tttaaccgca tgggataaaa aataaagtgt tccttttttg gaaaggtcaa
     1261 aacggtgatt atttttgcat atcaagccat tatcagactt ttttacttct tcttggcaaa
     1321 ccggacagcg gaacagctga tgattttctg ccaaaaactg gcgtcctcgg tcaatttttt
     1381 ttaacatagt tccccctcat ttctgtttta tcttaccgtt agatgctcac tttggcaact
     1441 gctaaaaata tgatacaatg cctagaagaa agtgaaagat aaaggagaag attagatgat
     1501 caaagaattt tgcgctgaaa actacacatc tattcctcta gcaattgcta gaggagccga
     1561 tcggatcgaa ttgtgtgaca acttagcggt cggtggcaca accccgagta ctggtgtgat
     1621 cgaagaaacg ttaagttatg ctggggaaaa agatatcccc gtaatgacga ttattcgtcc
     1681 tagagggggc gacttcgtct ataacgatat cgaattgaaa atcatgcata ctgatttgat
     1741 tgaagcaaag aaattaggga cggatggcgt agtatttggt tgtcttactc cttcaggctg
     1801 gctagacgaa gaggccttag aactattgat cgaaacagca gaagggttgc agatcacctt
     1861 ccatatggct tttgacagta ttcctgaaga tcggcaattc gaagcaatcg attggctggt
     1921 agaacatggt gttgaccgta tcttgactca tggcggtcct gctgacaaaa ctattgtaga
     1981 aaatttccca cggcttaaag aattgattgc ctatacagat ggccgcttaa tcattttgcc
     2041 tggtggtggt gtcactgcag agaatgcagc acatgtagct aaagaattga acgtaagcga
     2101 agtacacgga accaaaatcg tggatcttca gttataatct tataaacaaa aaaacatttg
     2161 caacaggcat aatgcttatt gcaaatgttt ttgttttttt acataaaata gcccataaat
     2221 aaagaagcaa tattgaaata aatcagtaca ctcgtcaaat cgctcaaagt cgtgataaag
     2281 ggaccgcttg ctactgctgg atcaaatcct agacgatcca ttagcatcgg gattaaacta
     2341 cctgcaagat tcgccactgt gattgcaaat agcatagcca tccctatgac aaaccccaag
     2401 ataaaattat gtttccaaaa acctacgatt agaaaaatcg tgatccccgt aacagctcct
     2461 gtgacaacgc cggtcaagac ttcagccaaa acaagtcgaa caaatccatg atctttctca
     2521 tcagaaatcg caagtcgacg aacagccaca gctagcgact gggttccagc attaccggca
     2581 gttcctgtaa tcagcgaaat gaagactgct aaaatactcg cttcacttac caagtcttca
     2641 tagtgactga ttaaagaagc ggtcgacata cccaaaaaca acagagtgat cagccaaggc
     2701 aaacgtcgag acgctgcctt tactggattt tcattaattt cttccacatt gaccccggca
     2761 agtcctgaat aatcgctagc agcttcatca tcaataacgt cgatgatatc atcgactgtg
     2821 acgattccta acaaatgatc atcataatcg gtaacaggaa gagctaagaa gtcataatct
     2881 ctaaatgttt gtgccacatc ttcttggtcg tcgcctacat gaactgaaag cactcgttcg
     2941 ctcatcagat cagaaatcat tgcgtcgtca tcattcacga ttaggtcgcg cagagaaata
     3001 acccctacta gctgaccttc ttgattaacg acatagatgt aatagattgt ttcagctaca
     3061 tctgcttcgt tcttcagcac gtacatcgca gagcgaacag tttgattggc tacgatcgaa
     3121 acaaattcag ttgtcatgat cgcgccagcc gtttcatcct cgtaatggag aagttccttg
     3181 atttcactgg catcgtctgc cgacatcaaa cttaaatatt tagcaatttg ctttttatcc
     3241 agcgtattaa gcaagtcaac agcattatct gtatacatct cagaaagcat atcagctgca
     3301 tagctaggac gcatttcatc taggtattct ttcatatgtt cgttgtcttc ttcaatcaca
     3361 tcgaacatat ccgctaattc tttaggcgac agataggaat agatcagctg cctgttctct
     3421 tctgtaagag attggtagaa ttgcccctgc tcatagatat gcatctctaa aaaactgttt
     3481 ctgaatgcct gaatattatt ttcccttaaa tcttgagtca actgggcaaa tctttcctct
     3541 aattcttgat tttcatccaa accaatctca cctcgcctta ttcttcaaac cgtctcacaa
     3601 ctgcctccat gtctgcggct aaaggctgct tgaactccag caactcttct gtaaatggat
     3661 ggtaaaaacg cagatcataa caatgaaggg cttgtctttt tattcccaga tccattcgcc
     3721 cgccatacat atcatcaccg agcagggcac agccaattgc ggaaaaatga actcttattt
     3781 gatgtgtcct tcctgtatgc agctggatat caactaacgc gatttctgtt agtcgcttct
     3841 ctatccagta ctcagtctgt gccgaacgtc cttcatcaga gaccattcgc ttcaatagcg
     3901 aagaataatc tcgggcgatc ggcaaatcga tatgtccatg tgccgataaa tctgaaagcc
     3961 ttccactgac taaggcctga tattttttca caaacttctt ttcacgaagt tgcatatcta
     4021 attttgcgtg cgcaaatcca tgctttgcaa aaagcatcaa accagatgta tcccgatcta
     4081 gtcgcgtcac cacatggatt acttggtttt cgtaaccttg ttttttataa taagccttta
     4141 cacgattagc cattgtccca tttggatggt attgggcagg aatagacgaa acgcctgctg
     4201 gtttatttac taccagaacg tgttcatctt catacacgat atcgatcggt gtttcatcca
     4261 aaagtacggt ttcatgttct ccttcagcag gaatcacaat ggttacctta tcatccttag
     4321 ctagagaaaa caagacattt tctacttgat cattaacttt gatttgaccg ccttgaaatt
     4381 ttattttcgc taacagaccc tttgagatcc ctttttcttt taaaaaatat tttacttgct
     4441 gagaatgatc tttctcataa tcccatgtaa actgcatatc aatcctcact aataaaggca
     4501 tctttcacac gatgccaaaa atgcatatgt cgataagaag caaaatggat ccgttcttct
     4561 gctattcgat aataaattga gcggatattt gcttttgcga cattcaattg atcaaccgtt
     4621 acaagataat cttctgtatt ttccaatttt atttctagcc attcatcatg ggcaatcacg
     4681 atcggagaac ccaaagtacg aaacactcga ttattcagtg aagcaatctc tgccaactga
     4741 aaggcattga tactaggatg gatcacagca ccgccgacac ttttattata tgctgtcgaa
     4801 ccagtaggcg tagaaatcgt caatccatct cctctaaaac tttcaaacag ctcgtttttg
     4861 atgtatacat cggctaccat cgtacgattg gcccgtttaa tcgttgattc attcagtgct
     4921 aaaaaatgct ggtccggtgt ttcatccaaa taactgatcc gaacatccag caagggataa
     4981 ctaacacttt gttcccggtt cgttcgtaag ctgtctacta gttcttccag ctcgtaatcc
     5041 cgccaatccg tgtaaaaccc taaatgacct gtatgcacgc ccaagaaacg cacatcattc
     5101 agacaatgac tgtagtgatg gaacgccgac aataatgtgc cgtctccgcc gactgagatg
     5161 accaattctg gctcatgatc atctcgttga atccctgctt gttctaaaag tgcgttcaaa
     5221 cgttctgtca cttctatagt ctttgcttct ttattatgaa cgatcgctac tttcatcccc
     5281 tgcctcctct ctttcgcttt ctttcttcaa aagcgttcga tagtagacat catctgattt
     5341 tcctcttcca tgggagaata accgctgtgc ttcttggatt tcttcacgga tactggacat
     5401 ctcttcatcc aactgatagg cagcttctgc cgcacgctga agtcgttcac tcatttcttc
     5461 tggaaaaacg ccttgatatt tataattcag cgaatgctca atcgttgccc agaaattcat
     5521 cgcaagagtc cgaatctgga tttccgccaa aattttcttc tcaccgctga tcagctgtac
     5581 aggatactcg atcaccagat gataagaacg gtagccactt gcttttttat ttgtgatgta
     5641 atctcgttct tggagaactt tcatgtcttt tcttttacgg atgatctcaa caacttgatg
     5701 gatatcttct acaaactgac acatgatcct taacccggca atatcagaca tttcttcttc
     5761 taaacgtccc attggaatat gccgtaattg tgattttgtt acaatactat cgactggctt
     5821 gactctccca gtgacaaatt caatcgggac atgtcgattt tgttcacgga actgtttgcg
     5881 gatcccccgc agtttcactt tcaattctga tacagtttgt tcatatggtg ctaaaaatgc
     5941 ttcccaatct cgttccatag ctgatacctc tacttcatta acattcattg tttattctac
     6001 catatatgca aagttttttc gtactcttga tttatgcatt gaccaaaaaa agaaaattag
     6061 gcaaaataga caaaaaggag tcgatcaaat gacacaaatg cttgaaatcg aatataaatc
     6121 tatgctgaca aagcaagagt acgagatatt catcgctcac tatcaattaa cagataaaga
     6181 tttccgtgta cagactaata tttatttcga taccgttgat gaacagctga agaaacaaaa
     6241 ttgtggccta cggatccgtt tcgttggtca tcaagctgaa tatacattaa aaacacctgc
     6301 tgaaaaaggg cgtctcgaaa caacggatac ctttataaca gaagaagcag aagcatttat
     6361 cttcgaaaaa cgtctgcctc gttcaggcgc agtttttcaa aaactttctg atttgaatat
     6421 cgatcctgct tcattaatcc aaaccggaaa gcttacaaca aaacgggctg aattccctat
     6481 tgaagaagga cttttagcta ttgatgaaag ctggggagac aatctacacg attttgaact
     6541 ggaactagag gtaggggatg catctgtggg aaaaattgct tttataaatt ttttaaaacg
     6601 tttttcttta ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg aagctaaaaa
     6661 ttagaaacct ttcattttca caaggcactt gagttcattt ttttactttt ttctgttaag
     6721 ttataggatg tgaaagaatc tagtcactag ttgtatagaa tctattttgg gggagattca
     6781 tatgatcgaa atctatttat ttgttaatcc tttaggggaa tactgtttcg aaatggaaca
     6841 acagttatta caatttatcg aagaagagta cgggacacca tcaaaagaaa agatgcaatt
     6901 tcgctttctt cctttggtca atttgcagac aattggggat gtcatgcaac aaaaaggaat
     6961 ttctcagaac aatcttgagg aacgtaatca cttattctca atgacttatt ctgcagcatt
     7021 ggactgcaaa gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt tgatcaaatt
     7081 gcaagaagca gtcggctgta atgacatacc atattctcaa gaacttgctg aatccatatt
     7141 tgaagaaatc ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg acttcgttaa
     7201 agaagtattc ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa aacacccttc
     7261 tgcagtcgtt tacaattatg cctgcgaccg tgattttggt gttttagtag aagtaaaagg
     7321 tacaagtagc ctttggagaa tcacagagct gtgtaaaaca acagataact catatcaagt
     7381 ttttaatggt gaacatttac atacaaatga ttctcagttc cattcaagaa ctagccattt
     7441 acacttgctt tctaattaaa aaaatacgtc gactccctta caagagagcc gacgtatttt
     7501 tttcgtttct attcgagaaa atcaaaaata gaaagcctta taaacgatcg atcaattctt
     7561 ctaattcatt tagacgttgt tcaaacatcg acattgcatc ttcaatatat gcagcctgtg
     7621 tcatatcaac tcctgctttt ttcattactt ccacaggata atcgctgtta cctgctttta
     7681 ggtaagccaa gtagttctct agtgcctctg gttcctggtt tagtattttc ttcgccagtg
     7741 ctgaagcagc agaaaagcca gtcgagtatt ggaaaacata gtaattataa taaaaatgcg
     7801 gtatccgtga ccattcgaat ttaatttctg ggtcttcttc gactgctgga ccataatatt
     7861 ttgcattcaa tttaccataa ctaccactta ggtattcact agtcaatggc acaccttttt
     7921 catcttccgt atgcatgaaa tgctcgaatt cagcgaattg tgtctggcgg aaaactgttc
     7981 ctttaaaccc atccaagtaa tggttgagta cataagcccg tacacgaggg tctttttctg
     8041 tttccaataa atactccgtt aggatatttt cattcgttgt cgaagcaatt tctgctaaaa
     8101 agatggagta gtcgccgtac acataaggct ggttcgaacg agtgaaataa ctatgaacac
     8161 tatgtcccat ttcgtggaca agcgtaaata gctgatccag cgtatcatgc caattcaata
     8221 aaatatatgg atttgtgtca tagcttcccg aagaataagc accgcttcgt ttccctttat
     8281 tttcgacaac atcgatccaa cgttcagaga atgctttttc tacgatggcc atgtattctt
     8341 cacccattgg tttcagtgct tctaaagctt tttcttttgc ttcttcgtac gtaaaggtaa
     8401 ttggagcttc ccctaagacc ggtgtataaa gatcatacat gtgcagtttt tccacttcta
     8461 ataaacgttt ccgtaattcc atgtatcgat gtaacaaagg caaatgcttg tttaccacgt
     8521 ctaccaaagt atcgtataca ctttcaggaa tatgattatt gctcaaagac gcttctctgg
     8581 cagagctgta attacggact ttcgctttaa aattatgtcc ttttatatgt gtgcctaaag
     8641 tagaagcaaa tgtatttcta aattgttcgt aaacactgta caatccctta aatgctgctt
     8701 cgcgcaccct tcgatccgtg ctttctagca actgaccata cacgccatga gataattgga
     8761 ctatttcacc attttctcct tcaatcgttg gaaaaactag atctgcatta ttcaaaacag
     8821 caaatgtatc acttgaagca tcaaagattt cacctgctcc agcaagaaga gattcctgct
     8881 cagcagataa gacatgggct cgattatcta ctatttgctg gatataatgg cgatagactt
     8941 ccaattttgg ttcttctttg aaatattgcc aaatctggtc atctgacaat tgcaatattt
     9001 ctggttcaaa ccaggaaacg gcttcactaa ctttcgaaaa cagactgctt gctcttgcat
     9061 aaagcgcttg gtaatctgta tttccagtgt cttgatcgtt tttaaggtgc gcatatacat
     9121 aaatgacttc agtttggcga taaaccctca atacgaattc aatcgcattt aaaaattgag
     9181 aagcgccttg atcaagtgtt cctttgtgtt tttcagattg ttttaactct tctgataatt
     9241 ccaaatattt ctcatcaaac tcttggtcgc ttgaaaagat cttggtcaag tcccaagtta
     9301 aattttcagg caattcttct cgttttggca actgctttac ttccatgaaa tccactcctt
     9361 ttcttccctt agctctatct atcaaaatag atcatagtct aacatccaca tgtcacagca
     9421 tctaactttt ctactcattc atgtgtctgc ttccatcaat tttagcataa ctaaatcttg
     9481 ttttacatag aaaaccgatt cctagcctat ttttatactt actatttttt caaagaagac
     9541 aagtgtatgc attcgttaaa aaactgctga tagaccatct cttcatcaat cataggaaaa
     9601 agccattccc ttttatgttc ttttatttgg caaagccatg agttgaaatc aggaactttc
     9661 tggttttcca aggcttcttc gtacaacatt cgataaagaa aaacctgttc ttgtaaataa
     9721 aagaaaaacc gacttttttg atagatccac gatgatagat gcaaaagatg tttatgacgc
     9781 aagtagcatt gttcttgtat ggatactgta ttcttttgct tgtgaataag ttggcgtgtc
     9841 agccatttct tcccatcttc actcactttt tctatttttt tcatttggtt cattttaatt
     9901 tcgttactag tgaatatttc tttaaggtcc ctactagaaa aagtccagca gctactgcaa
     9961 aaggaaatat gccctgatac agtctctgta atatgatgat acaaataaag cttttgtttc
    10021 ttgagatcag ctttccagca atgcactcct ctttttctat tatagtagca gcaacttttt
    10081 tctatcaatg agaattgcga ttgtccaaga aaagaattac caagtatcca ccatacttgg
    10141 taactgttat tcaaataatt ttgggttctt tcaataaatc tctggtgaga cagtctagaa
    10201 cattggattt ctattgctag attcgccaac aaaatatccg gcctttgccg caacctcggc
    10261 aaataggctt ccaaaaatac tggttctact gactgtccca gccaatccat gaagcattcc
    10321 tttaactgta agtgctcttt tgattctctt tcactaaaga ccgaacagtt atctgatttt
    10381 tgatgggcaa aatgagcaca ttttttcttt ccctttttca aaatcactct ttttcgacag
    10441 ccacgacaat aatatttctt tcctttttct gcatgtttag caaaacagca cttattatat
    10501 tcattcattg caagcagcat ttttctccct ctgaacttat ttacgtctaa gaatttattt
    10561 acacgaaaaa agagggcgtg aaaaagctat tctgcatcaa gtaataagaa cagctaaaca
    10621 aaaatagttc ccaatatttt tcgtttggtt gggcttgtta ccgcagatgc aagcttttga
    10681 acacatttat acgaaaaaaa gactgcgaca caatttttgt cacagtctct tactataatg
    10741 ctgatttata caaaataaaa tttcgttcgc tcgatagcat tacgcgccat cagacattgt
    10801 ccatattcat tcaatgtttc tggtgtacga tcgctctttt cagcaaactc caaaattcga
    10861 gcaatctgat tctctacatc cgcttggttg agattttcca tccagaagtg aacgactagg
    10921 taatagcgct cttcgaataa atacaaatca gtccaagcag actctagttc gacatctgtt
    10981 gcaagttgga tcacttgttc aaaatcttca aaagcaaaag ttgcttgtct ttcctgttct
    11041 tctagggcgc ttccagataa ttcttcctcc ggatcgctgc caataatctg ctgacgaaga
    11101 aagtcactca catcttctga atcaacagta gaatcttctg attgagaaat ttcttcatcc
    11161 ggaagatttt tactgataaa cagttctaat ccgtctcctt ttggcaaaac ttggaaagtg
    11221 acagcttcgc ttcctcggaa ctcctcttcg atatcaactt cttccaaaat actataaaag
    11281 aaattttcga tttctttgtg attgccaagt aaatccaaaa atgaaacgcc ccgttcctct
    11341 aaatcttcat gaccaattag tacacggatc gtattttcat taatatgttc catttccatt
    11401 ttgctacacc tcactttgcc acaatgttta atatagattc attgtagcga aatcagctta
    11461 aatgtaaaga aaaaaaatct cgctgttaag cgaatttaat aaaaccagcg gatttttcaa
    11521 cctttcaagc tatcttattt aactataaca tttttggcag acgaatacta tatagccgac
    11581 ttttcagcta attttccctc tctatttttc gtgcaatcat catcttctac taccttttta
    11641 tacatctaat caagaatact ttttgtaaaa acaaaaaaac ttggaaaaac tatctttgta
    11701 atagtctttt ggctctatac tttttgatgt tggtgaactt cacca
//
LOCUS       DACECJ010000087        10928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.087, whole genome shotgun sequence.
ACCESSION   DACECJ010000087 DACECJ010000000
VERSION     DACECJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.087"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..287)
                     /locus_tag="IWO09_002380"
     CDS             complement(<1..287)
                     /locus_tag="IWO09_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0264954.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="IWO09_002381"
     CDS             429..1547
                     /locus_tag="IWO09_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0264955.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="IWO09_002382"
     CDS             1675..3051
                     /locus_tag="IWO09_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0264956.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="IWO09_002383"
     CDS             3452..5032
                     /locus_tag="IWO09_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0264957.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="IWO09_002384"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="IWO09_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="IWO09_002385"
     CDS             complement(5658..5978)
                     /locus_tag="IWO09_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0264958.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="IWO09_002386"
     CDS             complement(6126..8360)
                     /locus_tag="IWO09_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0264959.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="IWO09_002387"
     CDS             8839..9105
                     /locus_tag="IWO09_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0264960.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="IWO09_002388"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="IWO09_002388"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0264961.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2215 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaa
//
LOCUS       DACECJ010000088        10817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.088, whole genome shotgun sequence.
ACCESSION   DACECJ010000088 DACECJ010000000
VERSION     DACECJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.088"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWO09_002389"
     CDS             complement(25..144)
                     /locus_tag="IWO09_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0264962.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWO09_002390"
     CDS             complement(159..1343)
                     /locus_tag="IWO09_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0264963.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IWO09_002391"
     CDS             complement(1599..1853)
                     /locus_tag="IWO09_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264964.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IWO09_002392"
     CDS             complement(1878..3020)
                     /locus_tag="IWO09_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ0264965.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IWO09_002393"
     CDS             complement(3080..4432)
                     /locus_tag="IWO09_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0264966.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IWO09_002394"
     CDS             complement(4503..4847)
                     /locus_tag="IWO09_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0264967.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IWO09_002395"
     CDS             complement(4857..5231)
                     /locus_tag="IWO09_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0264968.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IWO09_002396"
     CDS             complement(5244..5558)
                     /locus_tag="IWO09_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0264969.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWO09_002397"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWO09_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IWO09_002398"
     CDS             complement(8976..9638)
                     /locus_tag="IWO09_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264970.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IWO09_002399"
     CDS             10255..10485
                     /locus_tag="IWO09_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264971.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="IWO09_002400"
     CDS             10491..10790
                     /locus_tag="IWO09_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264972.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DACECJ010000089        10502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.090, whole genome shotgun sequence.
ACCESSION   DACECJ010000089 DACECJ010000000
VERSION     DACECJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.090"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(230..982)
                     /gene="xth"
                     /locus_tag="IWO09_002401"
     CDS             complement(230..982)
                     /gene="xth"
                     /locus_tag="IWO09_002401"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0264973.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1095..2015
                     /locus_tag="IWO09_002402"
     CDS             1095..2015
                     /locus_tag="IWO09_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0264974.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2088..3023
                     /gene="murB"
                     /locus_tag="IWO09_002403"
     CDS             2088..3023
                     /gene="murB"
                     /locus_tag="IWO09_002403"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0264975.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3321..4799
                     /locus_tag="IWO09_002404"
     CDS             3321..4799
                     /locus_tag="IWO09_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0264976.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5076..7136
                     /locus_tag="IWO09_002405"
     CDS             5076..7136
                     /locus_tag="IWO09_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264977.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7219..7788
                     /locus_tag="IWO09_002406"
     CDS             7219..7788
                     /locus_tag="IWO09_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0264978.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7963..8769)
                     /locus_tag="IWO09_002407"
     CDS             complement(7963..8769)
                     /locus_tag="IWO09_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0264979.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8907..10421
                     /locus_tag="IWO09_002408"
     CDS             8907..10421
                     /locus_tag="IWO09_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0264980.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3721 a         1754 c         2068 g         2959 t
ORIGIN      
        1 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg
       61 tttggctgtt cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg
      121 ttcaaaagcc agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc
      181 acaaaatttt tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat
      241 ctaattctac tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta
      301 gtcctggctc taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt
      361 tccttgaatt gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat
      421 aacggaatgt atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg
      481 gagtgaatcc agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag
      541 cgacatttag atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat
      601 tataaaaggc ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat
      661 ttggcgtgta acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt
      721 ctgtatcatg gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa
      781 aaatcgctgt gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg
      841 gaagatctaa atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg
      901 cgtctaactc atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta
      961 cgttccaaga gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg
     1021 cgatactatt gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc
     1081 caggaggaaa gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat
     1141 atttaccaac ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata
     1201 aagaaacaaa agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg
     1261 atgagctttt agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt
     1321 atgaattagc caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga
     1381 gtgtccatct aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt
     1441 tgatgatcgg tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag
     1501 ataagcggtt gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg
     1561 tcctttatga tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca
     1621 ttttgagaac aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac
     1681 aattagaaac acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata
     1741 tagaaaaata tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc
     1801 ttgtcattca agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc
     1861 tagtcaaacg cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta
     1921 aatcggaaac agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg
     1981 aagtgcttaa agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag
     2041 cgtgatagaa taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag
     2101 aaatagttga aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata
     2161 cgtttacgaa aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag
     2221 taaagcaaat catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg
     2281 caagcaatct gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa
     2341 tgaaacagat tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg
     2401 atacgactta tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc
     2461 cgggaagtgt cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag
     2521 atgtttttgc tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag
     2581 acgaaatggt attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg
     2641 aagctcgatt cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt
     2701 tgaccgaatt gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta
     2761 agcgcccagt aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga
     2821 aatggggcgg tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg
     2881 cgacagcgac agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat
     2941 ttgatgtgtc tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa
     3001 caagcagaag tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac
     3061 ggtggccgtc ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt
     3121 taacacaata aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat
     3181 attctcaaaa aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca
     3241 taaaatcagt ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg
     3301 cattttttga ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa
     3361 aactgctgct agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt
     3421 tatatgaaac attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata
     3481 aagaatttac tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc
     3541 atgtcgaacg tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat
     3601 caaaaattgc tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg
     3661 cagagaatca ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt
     3721 ttcttgaaca gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca
     3781 tacggaacgt attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta
     3841 gagaaagcgt catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt
     3901 taaaactgtc tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga
     3961 gggcctatgg gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct
     4021 cagaaggaat gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag
     4081 agcagatcat tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg
     4141 aattagatgg cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc
     4201 aaaaaatgtc agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag
     4261 aaagactata tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt
     4321 ggatattcga ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg
     4381 agacgtatcc aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt
     4441 tcttgggaaa agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg
     4501 tcggtatttg tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc
     4561 aaggatctgc ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata
     4621 ttgtgattga gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg
     4681 aaaaatttaa taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat
     4741 attttggaaa taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt
     4801 atttatggat ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga
     4861 aaaggtaact agatataact agatataact agatatatta cttaacaaaa ttaaaaagta
     4921 cataagttat tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa
     4981 tagtccatct tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt
     5041 catgtttaga caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac
     5101 aactaaataa acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg
     5161 ttaagtttat gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac
     5221 ccgttcaggc aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg
     5281 attcccctat cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg
     5341 cattgacttc ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc
     5401 ctaacaaaac attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg
     5461 atatgactga tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg
     5521 ctttgacaga tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt
     5581 attcacagtt tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg
     5641 atctggtaga aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt
     5701 caaacaatgg gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct
     5761 attttgatcc aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg
     5821 atcaaacagc tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta
     5881 aagatcttgt tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca
     5941 aaatgaacgg gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata
     6001 tagactcgaa aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg
     6061 ctgcttcatc gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag
     6121 cagaaacaca tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt
     6181 ctgatcgagt gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta
     6241 tttctgattg gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg
     6301 aagtacttca ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta
     6361 cttatgttta tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa
     6421 acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg
     6481 taccgttaaa ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga
     6541 ccaaacaaac aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag
     6601 aactccatgc tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag
     6661 aacaaaaaag acgagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt
     6721 ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa
     6781 tctcagaatc taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat
     6841 tggaaaatga aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta
     6901 aacagtcgga agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt
     6961 caactattga ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa
     7021 cacccgcacc tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg
     7081 caggaacatg ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta
     7141 tatctctatc tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag
     7201 acgaggaggg atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca
     7261 agaccacttc aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt
     7321 tatcaaaaag aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa
     7381 acgacagatg aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt
     7441 gaaagaagga tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct
     7501 tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca
     7561 gattaccaag gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct
     7621 accctccata cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt
     7681 gctctttata aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat
     7741 ccagattatg aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa
     7801 ataagtcctc agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg
     7861 agttaatact atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac
     7921 aaaagtattg tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt
     7981 aggaataaaa ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc
     8041 aaatacactg aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat
     8101 gattcccacg tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt
     8161 ttgactttca atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt
     8221 acttaactcc acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag
     8281 taatttatct gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca
     8341 gaaggaaaca actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag
     8401 caaataaaca agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa
     8461 agtatcccac attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact
     8521 aaaaataaaa gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt
     8581 ttccagaaaa taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc
     8641 ccagtgttgg ttccagacct gatccactag cataataacg acgatgatac gtaataaaat
     8701 atagattccg tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa
     8761 atgattcata aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta
     8821 ccaaagctat atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat
     8881 agtataagga gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg
     8941 ttgcacttct tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt
     9001 ctagtacgaa ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc
     9061 caaaagaaat gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg
     9121 cagcagctat cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg
     9181 ttgccggagg aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc
     9241 aaaaagaaga aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg
     9301 gtggaggaac gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg
     9361 tcgattggct agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga
     9421 gtgaaaagag gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg
     9481 ggttattgaa taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag
     9541 acattaagaa aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac
     9601 caaaagaggt caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag
     9661 aatttattga aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat
     9721 caactggaga tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata
     9781 aaatacaaat tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc
     9841 gcggagaagg agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata
     9901 caagggataa agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt
     9961 ttgaccaagg cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg
    10021 tcacaactgg aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac
    10081 tagcaaaaac agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca
    10141 accgtgccac agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg
    10201 caccaggaat ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag
    10261 ataaagaaaa ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac
    10321 atggaaacaa ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag
    10381 cgggcataca agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa
    10441 gaggttgtga aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta
    10501 gt
//
LOCUS       DACECJ010000090        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.092, whole genome shotgun sequence.
ACCESSION   DACECJ010000090 DACECJ010000000
VERSION     DACECJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.092"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IWO09_002409"
     CDS             complement(247..759)
                     /locus_tag="IWO09_002409"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0264981.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWO09_002410"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWO09_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0264982.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IWO09_002411"
     CDS             complement(3212..3646)
                     /locus_tag="IWO09_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0264983.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IWO09_002412"
     CDS             complement(3656..4444)
                     /locus_tag="IWO09_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0264984.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IWO09_002413"
     CDS             complement(4441..5382)
                     /locus_tag="IWO09_002413"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0264985.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IWO09_002414"
     CDS             complement(5559..6146)
                     /locus_tag="IWO09_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0264986.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWO09_002415"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWO09_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0264987.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IWO09_002416"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IWO09_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0264988.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IWO09_002417"
     CDS             complement(9063..9998)
                     /locus_tag="IWO09_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0264989.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACECJ010000091         9618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.093, whole genome shotgun sequence.
ACCESSION   DACECJ010000091 DACECJ010000000
VERSION     DACECJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.093"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            120..1094
                     /locus_tag="IWO09_002418"
     CDS             120..1094
                     /locus_tag="IWO09_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0264990.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1076..1537
                     /locus_tag="IWO09_002419"
     CDS             1076..1537
                     /locus_tag="IWO09_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0264991.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1587..2051
                     /locus_tag="IWO09_002420"
     CDS             1587..2051
                     /locus_tag="IWO09_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0264992.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2065..2814
                     /locus_tag="IWO09_002421"
     CDS             2065..2814
                     /locus_tag="IWO09_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0264993.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2814..3644
                     /locus_tag="IWO09_002422"
     CDS             2814..3644
                     /locus_tag="IWO09_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0264994.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3644..4291
                     /locus_tag="IWO09_002423"
     CDS             3644..4291
                     /locus_tag="IWO09_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0264995.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4389..5165)
                     /locus_tag="IWO09_002424"
     CDS             complement(4389..5165)
                     /locus_tag="IWO09_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0264996.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5309..6268
                     /locus_tag="IWO09_002425"
     CDS             5309..6268
                     /locus_tag="IWO09_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0264997.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6385..6693
                     /locus_tag="IWO09_002426"
     CDS             6385..6693
                     /locus_tag="IWO09_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0264998.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6720..7019
                     /locus_tag="IWO09_002427"
     CDS             6720..7019
                     /locus_tag="IWO09_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ0264999.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7193..7744
                     /locus_tag="IWO09_002428"
     CDS             7193..7744
                     /locus_tag="IWO09_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ0265000.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7890..8183
                     /locus_tag="IWO09_002429"
     CDS             7890..8183
                     /locus_tag="IWO09_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265001.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8176..8472
                     /locus_tag="IWO09_002430"
     CDS             8176..8472
                     /locus_tag="IWO09_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265002.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8543..9547
                     /locus_tag="IWO09_002431"
     CDS             8543..9547
                     /locus_tag="IWO09_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0265003.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3407 a         1373 c         1797 g         3041 t
ORIGIN      
        1 aaattgttat aaaaaaattt ttttgaaaaa tagtattgca aaataaagca atttcataga
       61 taataggtat tgtaaagtta tatgacttta taatacttat tagagtgagg gataaagaaa
      121 tgagacaact acacaaagaa cagattgaaa cagcaatggc agcgttaaag gaaaagacaa
      181 ttgatcatgt atattttaca gcatgtgggg gttcacaagc cgttttaatg gctggtcaat
      241 atctatttga taaagaatta actgtacctt gtcatgttta tactgctaac gaatttaact
      301 atgctatacc aaaaggatta tctgaaaatt ctttagttat cacttgttcc cattctggca
      361 caacaccaga aactgtagaa gcaacaaaaa aagcaagaga atttggagca atgactattt
      421 gtttatctaa tgaagaagga tctcctctat gggaagcagc tgaatatcct gttcactatg
      481 attggggtaa agatgttgat gctagtgatt taaataaagg aattctttat ggtttattat
      541 ttaatatttt acttgtttta gatggctcta aaaaatggga aacttgcttg aatgaatttg
      601 ataagttaaa tgatcttgtt attgctgaaa agagcaaatt tgaaataact gctaaagaat
      661 ggggtaaaaa ccaaaaacga gataaaatta tttacacaat tggtagcggt atcaattacg
      721 gagaaattta ctccacagca gcttgctggt tcatggaaat gcaatggatt aattctaatg
      781 ctattcattc tggggaattt ttccatggtc cttttgaaat cactgattat gatgtaccat
      841 tcatcttagt gaaaagtatt ggagatacaa gatttttaga tcaacgggta gaagacttca
      901 ctaaaaaatt cagtaataaa ttaatggttt tagaccaagc tgattttgac ttagaatcta
      961 tagatagtgt agcgaaagaa tatattgcag ctgtacttag tggcgttgtt attcgccaaa
     1021 tggtagaagc gattgctttt gaaagaggcc attccttaga tgttagacgc tatatgtggc
     1081 aaatgaaata ttaattaata ggaggttgca aaagatggca aatcaattat ttcacattgt
     1141 gattacaaca cattctaccc tttgtgaggg ttatttaaag gcaacaaaat taattttaat
     1201 gaatgacgcc acaggtatta gtgctgttcc atttaaagaa aatactagtc cagatgattt
     1261 tgaggagagt atgaaagaaa taattagtca gcatcatttg caacctctaa ttattttaac
     1321 ggatttaatt ggaggaacac ctaataatgt ttctattaaa cagttgaaca acaatagaat
     1381 tcaaattatt gctgggatta atttacctct tttactggag ttattaatgg cccagtcagc
     1441 tggtaaatct atagaagaaa tagattttaa ggaattactg aataactctc aaaaaagtat
     1501 ggttcatatt aattcgcttt taaaagatgt cagttaaggt ggcaatttct attctataaa
     1561 ttttaaaaat tgaaggagga aatagaatga ttaaattagt gcgtgtagac cacagattac
     1621 ttcatggaca agtaatattt tcttggacta aacagtatga tattaaccat attattgtgg
     1681 ctgataacaa agttccagga gatccaatgt ctgtgatggc tttgacaatt gctaaacctg
     1741 ctgattgcaa attagacatt gttaaaatta gtgatgttaa agggattatg gaaacatccc
     1801 caaatgataa ttacatgatt ttaattaaag gacctaagga agcttttgaa ttagttcaaa
     1861 atttaccaga aattaaagaa attaattttg gcggtgtgtc taagaaaagc gagtccaaac
     1921 aatatggaaa ggcagtcttt ttaactcctg aagaattaga agccacaaaa aaaattcttg
     1981 atcaagaagt gaaaatattt gtgcaacaag tcccatcttc aagtattgaa aatgtagatt
     2041 ttagtaagta aggggggaaa ataaatgtta ttaaaagcca ttttattagg tttagttggt
     2101 gtggtagctg ttattgactc caggttaatt ggtcgacaaa atattggtag accactaatt
     2161 ttgagtacac ttgccggact tgtgctaggt gacttgcata cagggattgt tcttggggca
     2221 tctttagaat taatttctat gggattcgtc tcaattgggg cggctggacc tcctaatatg
     2281 caacttggta gtattattgc cactgctttt gcaattttaa ctaatagtag tacagaaaca
     2341 gccttaacaa tagctatccc agtggctgta gctggtgaat ttatgagtat tatcatgcgc
     2401 atgataattg ctcaatttgg acatgctgca gataaagcca ttgaaaataa taaatttaga
     2461 aaagctcaaa ttattcatat ttattggtca tttattttta atgcttttgt ttactttatt
     2521 ccaatctttt taacagtata ttttggtgct gacgttgtgg cagatttagt agataaaatt
     2581 ccagaaatga ttacagacgc cttgactgtc tctggaaata tgctttccgc attagggttt
     2641 gcaatgctat taagcacaat gttgagtaaa aaattatatc cttactttat ttttggattc
     2701 tttattgttg cctattcagg tctttcacta attggcgtga caatttttgc agctctgatt
     2761 gcctttgtaa tggatcaagt taaatatgga aaaagagaag aggcgcatgg ttaatggaaa
     2821 atagtacaga aaaaaaacgt ttaatcacaa aaaaagatct taataagatg tgtcttcgtt
     2881 catggacaat catgtgttcg tggaattatg aaagacaaat gcatatggga tttatgtatg
     2941 ggatggcgcc aattttaaat aaagtttata aaaatgatga agagaaaaaa agagaagctt
     3001 atactcgtca tatggcattt tttaactgta cgcctcaaat gacttcattt attatgggac
     3061 tttgtgcttc tatggaagaa caaaatgctc aagcagaaga aggaaacttt aatactgaat
     3121 ctatctccat gattaaaacc agtttaatgg gtccttttgc tggaattggt gattcatttt
     3181 tccaaggtac tttaagaatt attacatttg ggattggatt gtcttttgct aagcaaggta
     3241 gtattttagg accgatttta gctgttggcc tttttgctat accttctatt ttagctgttt
     3301 tttacggcgg taaattagga tatgagtctg gcaataaata ccttactaaa ttgtatcaag
     3361 aaggtcttat ggatcgagta atgcaatttg cttctatcat tggtttggct gtagtaggtg
     3421 gaatggtggc tagcatggtg ccaattgtca cccctttaaa attcactaca gggggaacag
     3481 aacttgtttt acaagactta cttgattcca ttattcctgg attattacca cttgtatgta
     3541 ctttgggaat atataaattg attaagaaaa atgttagtac aaatatgctc ttattaggtt
     3601 gtattttggc agggtttgtt ttaagtataa taggtatctt ataatgatta aggctctaat
     3661 ttttgatatg gacggagttt taattgatag cgagattatg tatcaaaagt gggtatttaa
     3721 ttttttaaaa caagaaaatt taagctatcc tttgtattct tatcaaaaaa agattggaac
     3781 tacagcaagt atttttgatg atatagaaaa gtacaatgag ggatgtaaca gtttagaatt
     3841 gaagggaaaa tttactaaat attggaaatc gcaaaagatt gattacttgg atatttttcc
     3901 taatatacta attgaacagc ttgaatggtt aaaaaagaaa aaaataaaaa tggcaattgc
     3961 ttcttccagt ccaatggata ctataaatga agttgttacc acttgtaaaa tagagtcata
     4021 ttttgattgt ttaattagcg gaagagattt gccagaaagt aaacctaatc ctactatatt
     4081 ccttaaagct tcagaacagc tcagaattcc agttgaagaa tgtctggtga tagaagattc
     4141 ttataatggt ataaaagctg gtaaaagagc aaatatgaag gttcttgcaa ttaaagataa
     4201 acgtttttct caagatgtaa gtctagcaga tgatgttatt tacgatatta aatatctgcg
     4261 acaagaagta caggtgaaat ttggaatata aataagtttt ttgatgtatt tagagaatat
     4321 tagtaaaaat aaaaatcatg cccaaatcta atcaaaattg attgctttgg gtatgatttc
     4381 ttttaatatc aatctattac tttaggatct gaatcatttt tatgcaccgt atctaaagtg
     4441 atattttctg tgacttcaat aacataacta attctatcac aacgaactgt ggattgagaa
     4501 taatgaaatg gttgattatt tttatctttg ccaacctttt ctaaaataaa tagcggatct
     4561 cctactatac attgcaataa gtcggaaagc tcaggtgttg cagataaacc gtttatttcc
     4621 ataatagagc tcgtagcttt gacatcatag tcatcttcta gaagtttata taatgaagta
     4681 tattggctac ctttttcaaa taaagttgga aatttttctt ctgatgcgaa aaaattatca
     4741 attcccatcg gggcaccgtc ttcccacatt aaacgtttaa tattgtaaac tctatcagtt
     4801 acattgattt gtaatttatt agctaaaaac tcatcagcct ttataatttt agaatgtaaa
     4861 acagtatatc taattttatg tcctcgggta gaaagagaat cgctaaatcc atgaactcca
     4921 gctaatttta aactttcttt tttttgagtt acaaaggtcc ctttccccct taatcgttca
     4981 actgttccac tttctgctaa aagtttaaaa gcttttcgaa tggtaatctt acttactcca
     5041 taaattttac ataattcttc ttctgtaggt aattgctctc ctggaagata ttttttttca
     5101 cgaatatcgt tacgtataga agcttctact tgtaaatata atggtctatt atcttgaaaa
     5161 ttcatagtac actccctaaa aagttatact tcattatatc acaatttatt aaaacgtcga
     5221 ttgtaaaatt aagctagaca actaaaaaga tcatttgtgc tacactcaaa atagttccga
     5281 ccaaagaatt aataaggagt gagtacaaat gacttacacc catcttacaa cagacgaact
     5341 agtaatcata gagtcttatt tcaatatcga gaaacctgtc gctcttgtcg cacaaagttt
     5401 aaatcgctct agacaaacca tttataatgt ttattcgttt ctcaaacaag gaaaaaaagt
     5461 gctagattat taccgtcaat acaaacaaaa taaatctcgt tgtggacgac gttcaaccgt
     5521 tttatctgat gaacaatcaa catatgttca agaaaaggta gctcaaggat ggtcacccga
     5581 tgtgattatc ggccgtggcg agtttccaat tgattgttct gttcgtacac tctatcggat
     5641 gtttaaaaaa ggcctttttg acacacagtc gttacctatg aaggggaaac gtaaaccgaa
     5701 taatcatcaa gaaaagcgtg gaaaacaggc ctttcgcaga acgattcatg aacggaacag
     5761 tctctatcca cacctgacta aagaatttgg tcatctcgaa gtcgacacca ttgtgggagt
     5821 taaacacaag agcgctgtta ttacattggt tgagtgttta tccaaggtta ttattacttt
     5881 gaaaccgaca ggcagacgag ccgaagatat cgaaaacagg ttaaatgatt ggttcaaatc
     5941 catgcctaaa cacttgttta aatccatgac atttgattgt ggaaaagaat tttccaactg
     6001 gcgatctata agtaactcga acgatattgc catctttttc gcagatccag gaacaccttc
     6061 gcaaagaggg ctaaatgagc actcaaacgg cttgcttaga cgagatggct tacctaaaac
     6121 aatggacttt agggacacgg atgaaacctt tattcaatcc gttgcctcga aaagaaacca
     6181 tattccaaga aaatcattaa attatcgaac accattggaa gtatttttga gctgcataga
     6241 taaagacata ttgtctagct taatttgaca aatcagatag tataaaatga atcaaattga
     6301 atatcaaata tatattatat ccatgaagcc caatcttctt taatagaaga ttgggctatt
     6361 tttatggcaa aggagggaaa agaaatgttt caaaataaag gacaacgtta tgtcacaaag
     6421 ggagtaatgg acagtttgcc ggtggaatta caagctttat gttggaatct aatcgatcaa
     6481 aacgttcaaa agcaatttca tttagactat cttcaaatct ttgaattttc tacggaaaaa
     6541 ggaaatcaaa agttggttca tcgtcaagaa gaaccagaag caagaaaaga gtatctgatt
     6601 tcacctaagc ttcgtttgaa atcagtgtct caaaaaatat gggtcattga ttcgggtgag
     6661 tatcaaacga tgttattacc agaagaatac tgatatctaa aagaaaggaa cgaaaggact
     6721 tgttagaaac ggctatcgaa gtactggaaa agtgtgcaca gctagtcact actcctgaag
     6781 agtggggcta tgaatccgtt acgatggaaa aagaggaaat tgaaatgggt atgcttcctg
     6841 aagatgttac tctaccacgt ttagtgatga cacatcttta tatttattgc gcaccagaag
     6901 atggaaaaga ctatgtagtt tatttcatta cagatattac cagccagcga gaatttgttc
     6961 gtgggttact ggtagaagga cgattggttt ggagtcagat tggaggaacg aatgaataag
     7021 atcgattatt tgagaatgat tgcgaaatgt ttagaagata tccttacagc gattcatcaa
     7081 attctgaaga aaaaaggaga aagaatatga aatattttca actagtattg acacttataa
     7141 cggcaatggt gattgaattg cttgaacaaa aattagcata ggagaaaaag agatgattga
     7201 aaaagaatta gtgaccacac aaacagaagt ttggcgagat cctcatcata cccatcgcta
     7261 tgttttcaaa cgacaatggg cgatcaaagg aaaagaagcg gaagataaat tagcggtagt
     7321 cattactatt cgtccaacag atacagaacc atttacgaat gatttgagta tgttattaat
     7381 cgaaaagaat atccgacaat taggatttac tggttttatt gctgtgaatc ttttttcgta
     7441 tacaaaagcg aaaagtcctc gggtatttcc tcgtggaaat gatgaacaat ctttggaaat
     7501 attaacaact gttttcacag aaaagaaaat caacaccatt atttttgctt gtggttcgat
     7561 tacagcgacg agtcaagtgg cttatgaaca agcgaagacc atttacgatc aattgagtgc
     7621 gaaacaaaaa aagatgacca aagtattgat aaatgcagaa ggtaagttat cccacccact
     7681 aagtattcat gttcgtaatg aatggcaatt ggcagatagt caattattat ttcaagaaaa
     7741 ataacatttt attttcttat gttacttata agtagtttta catgcttttt gactttaaaa
     7801 atacatgtga tgatacatgt tatatgaatt atcaatgtac ccagtaatat gctattttca
     7861 ttatgtaaaa aataacaggg ggtgtgtaga tggagattga tgaaacttgt ttgagtaact
     7921 cttataaaag tatgaatgac ttttttgagg aaatggaact ggattcagaa ttgctctatc
     7981 aattttatct tgcttttcga gggcaacaga tttcttttcc aatgaaaatg tacgatcgtg
     8041 agttagttag aaaaagaatc gaaaacatga tagaacagga aaaaactgta gatatccgtc
     8101 aattgacaga agtttatggt ttctctgcaa gatggattca aaaagtaatt aaacaaaaag
     8161 aaaggagacg agtacatggc taagctgatt gtattaggtg gtttaattta cggtggatat
     8221 tggttatata aatatatgct tgcgcatccg aatatagata aaggaaaagt aattaagaca
     8281 gttggggtaa tcatagttgg actcatagct ctaactaaga aaaataatac taatagccca
     8341 aaaataatga gaacttatcg ctgtagagga tgtggaaggg aaatttctac tagttttaat
     8401 ccatctgaaa gccctcaagg aagagatcgt tgtcatgctg gctatcgaca ttcttgggaa
     8461 aggattaact aaatgaacac cattgagatt aattgactga taaaataagt acgcgaggga
     8521 gaaattgaaa gggggaaata aaatgaaaaa atggttgtta ttactaattg gaatggggat
     8581 tttattcact ggttgtacat ctgagaatac gaggactaaa gagactgtag aaactgatac
     8641 aacaattgag gctaaaaaaa gtaaaacgca atcaactgaa caaactacaa cagaaatgaa
     8701 acaatctgtg gaagcaagta caagtgaaag ttcaatcatt gaatcttctt ctgaagaggt
     8761 taaaaatact ttgtgggata gtgagaaatc tactcaacta gcaatttttg tcccacaatg
     8821 gggaaagaca ttaggtcaag aatataaaag ctacaatcct cagaacaatg taagtctgta
     8881 tggtacgcct ttaccaagtg cagttattga tggaaattgg aaaatggcaa tcaatgaagc
     8941 accagttacc gtacaatggt cagaagatgg aactggtcat gcagatttca atttagtagc
     9001 agtttattct gatgtagaaa ctggagaata tttagggcag catgtatatt tctttggctt
     9061 tcaaaatgga caacctaaag tttatttaac gcaacaaaat caaggcaatg ataaaaacta
     9121 catgtacttc aatgaaactc agaaccaaca gttaaaacaa ggtttcattg atattgtgaa
     9181 tggacaaact cctcaaactc cagtagtaga agaatctact caacaagcaa caactacaac
     9241 gacacaatct acaagtagct cagcaatttc aagttgggaa gctgctaaag aaactgtact
     9301 aaataataaa gctttatggt tgtatgattc tcctgataaa aatgcatcaa atactaattt
     9361 aattagcaca aatgaatata tgaaagatct aatgcaagat gaaaatggaa gctactattc
     9421 aattaatatt gtaacggatg ataatggaaa tacaacaatg gctggtggaa aacaattccg
     9481 tgtttataca gatggaagaa tttctcaacg tattggaatg actaacaatt ttcaaatgat
     9541 taaataattg aaaatattat ttatcaaaga aaaactgatt ttgagaaaaa ttatttaatt
     9601 ttcttagaat cagttttt
//
LOCUS       DACECJ010000092         9387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.094, whole genome shotgun sequence.
ACCESSION   DACECJ010000092 DACECJ010000000
VERSION     DACECJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.094"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            203..1078
                     /locus_tag="IWO09_002432"
     CDS             203..1078
                     /locus_tag="IWO09_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265004.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1223..1375)
                     /locus_tag="IWO09_002433"
                     /pseudo
     CDS             complement(1223..1375)
                     /locus_tag="IWO09_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1643..2974)
                     /locus_tag="IWO09_002434"
     CDS             complement(1643..2974)
                     /locus_tag="IWO09_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0265005.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3123..3836)
                     /locus_tag="IWO09_002435"
     CDS             complement(3123..3836)
                     /locus_tag="IWO09_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0265006.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4011..5435
                     /locus_tag="IWO09_002436"
     CDS             4011..5435
                     /locus_tag="IWO09_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0265007.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5734..6441
                     /locus_tag="IWO09_002437"
     CDS             5734..6441
                     /locus_tag="IWO09_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0265008.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6428..6751
                     /locus_tag="IWO09_002438"
     CDS             6428..6751
                     /locus_tag="IWO09_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0265009.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6840..7877)
                     /locus_tag="IWO09_002439"
     CDS             complement(6840..7877)
                     /locus_tag="IWO09_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0265010.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8198..9181)
                     /locus_tag="IWO09_002440"
     CDS             complement(8198..9181)
                     /locus_tag="IWO09_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0265011.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT         2942 a         1803 c         1601 g         3041 t
ORIGIN      
        1 gataaataaa aaacaaaaaa agttccatct ttaaaacacg ctattctttt actttttctc
       61 acattctcta agaaaagaaa aggagaatga atttttgaca attcgagcaa aagtgattat
      121 caaatatgta aaatcagcta cttttttctt aaacgtttct ttatactaaa tataaataag
      181 tgtttcatga aaggagaact agatgaataa ttggcttgag tattttcctg aaaacgtttt
      241 ggaacgcggt tacagctatc atctgcacgg ttttgtccgt catttgaact atacaagcaa
      301 gtacttgtcc gcaacagttt ctggcacgga agattataag gtagtgatca cttgggatga
      361 aaagaccaac atgacttgtg attgtctata tgccatagaa ggaaaaaaat gcaaacacat
      421 ggctgctgtc ttattcgctt acgaagaacg tccaataaaa aaatccaact actcactttc
      481 cgaactctcg tcccttgttt cctccgctag ttcctctctt gtacgggagc ttttaacaga
      541 gattcttata gaacatccgc aatttatcga acgcttcaaa gttaagatgc catttcatgc
      601 aataaattat tcagataaat taacaactat catccataaa tacgatcata ttatcaagaa
      661 aaacaaaaac aggaaaacag cgaagttcat catggagatg cgaaagttca tccaagaagc
      721 agttgaatcg ctcattcagc aaaatgccta tcttcccgca ttcgaattga tcaatgaagt
      781 gattgctact ctcgaaacat tctattggga gcctgaggat gaacgaaccc ttcttctgat
      841 agaggactgt tattatttat ggaaagaatt attagcagaa gcacctcatg cagaaaaaag
      901 gcaaatgttc tcttggttcg tctgccaagt cgatcatact gacgcctctt attcaaaacg
      961 ctactccata aaaatcctga aagaagactt tcgggaaaaa gaattttcta accaaaagaa
     1021 aaaaatagat aaaaaaacta aagaaacggt aaagaaagac gatttcaatg aaaaatagca
     1081 agtaaacgat aacaaccatc ctaaatccac aagaaataca gcagaccttg ctttaaaaac
     1141 acgaatcatg caaaggatga agtaaagaag acttaacgcg tacccaatat tagtcatttg
     1201 actattaggt acgcgttttg ttttataagt catgacaaat ttccttcgtt gctgggtaga
     1261 ttttccggca ttgttgatag attttatgat acttttctac attttgtaag agaggttcca
     1321 ctgcatctgt atattggata aaattctgac aacaactttc aggtgtgtcg aaccactcta
     1381 atctttaaag ataaatatta aaataacgat taataatatt aacagtcttt atttttcacc
     1441 accaaaaacc tatctttgca tatactaaat agacgtatat acacagatag gtttgtcttg
     1501 tgaaaaataa tgattatcct taccaaaaaa tgtttgtttt tctacatgtt cgtacttatc
     1561 aaaaaatatg atccttattg aaattactgg ctatttatat tccaacgact tcttgtgtta
     1621 atttttgtta tctcatagta aattatgttt cattttcact taatttagtt gtcacgtgtt
     1681 cgtcaattcg taaaaacggt aaatatataa acacacttgc ggctataatt aatatttgta
     1741 aaacagctgc tcgccagtct ccggctgttg ctaaaaatcc cgatataact ggtggcgtag
     1801 tccaaggaat ttgcactact acatgattaa ttaatcccac agcagttgca aaatatgcta
     1861 ctgtcaaaga aaaaatcggt gcaagcacaa agggaataat cagactgata ttaaatacaa
     1921 taggcaatcc aaaaataata ggttcgctta tattgaatat agcgggagtt accgataatt
     1981 tagcaaagtc cttataatct tttctacgac tgaagataaa tattgcaatc aataaagcaa
     2041 tcgtatttcc cgatccaccc attaccgcaa aggtatcttt aaaggccata ttgataatat
     2101 gtggaatttg ctctttgttt gcataagctg ccatattttc ttgcatattc tgaatcaaaa
     2161 acggatctaa caatgaacct gatattactg cttgatggat accaaatcca aaaaatagat
     2221 tagcaataga tgtaattaac aaaaatcctg gtaaaccagt tgtaatttta cttaatggct
     2281 ctgtaactat cgtggtaatt agcttattta tatccatatt aaacaattga atgattataa
     2341 atgacaataa agcaaacgag gtaatcgtaa taatgattgg gattaataca ttaaaagact
     2401 ttaaaacagc gggaggaata tttccggata gctgaatctg tagtttctta ttattagaaa
     2461 gtttaataaa taattctgta gccgctaatc caacaaatat acctacaaac attcctgaag
     2521 ctcctgtata ggatataggt atcacacccg tcacttcaac agctttatcc gcattttcag
     2581 gagtaaacat catctgcata ggtgttaata caacaaatgt cgaaatagca actaataccg
     2641 ctgcaatatg atttgtataa tttctgcttg cacacatatg ataagatact gctgctacaa
     2701 ctaataatgt agttatgctt aatgtaccat tggcaattga agtaccaatt tgttgtaacg
     2761 tggtcaatgt actagtatcg acaatttccc ccatgaatcc tgttggttct aagatcactg
     2821 aattaataag tgtcacaaat cctgctaaca tcataaatgg cataaatgta gcaaacgcat
     2881 ctcgcaaagt tcttaaatgg atctgattcc ccaacttagt cgcaactata ccaaattttt
     2941 caaatacaat gtccttcctg ctacttttgc ccatattttt tctcctttat gcttattgta
     3001 atcgattact ttttccaccc aaataataca tcacttatca aaaaaagtaa atacttttaa
     3061 ttaaatatat atatcttttt tagtataaaa aaataagctc tgaaaaaata attttcccga
     3121 gcttattttt caacaaaaac agcttcaaaa acaaagtctt gatataaatg tctcgatacc
     3181 gaatactcga aaggtttacc agacgataga gcaacaactt tgtctatctc aatcataaat
     3241 tcacctggat ctatttttaa tatttttgcg tcaaattcat tcgcagtatc tcctttgata
     3301 cgtatgtgcg aagtctttat ttccagaccc agttcatttg tgatataagc atatacagat
     3361 ttctttaaat gttttggttc caagcctggt atgactgcta atggcatgaa tgtttgctca
     3421 atcgagtatg gttgacaatc aataaaacgg cttcgtacaa tattatatac atagtctttt
     3481 acactgatac ttaatttatc agctactttt ttcgatggaa gttcaattga gaaagtatgg
     3541 atttctgaat ctacgtgttc ttcaccaacc acgctaaaca aaccttcgtt tggaccaaga
     3601 actcgtgagt tatcaattgg tgttctgacg acttcagtcc catacccact tctacttcgc
     3661 aaaactcctt cggaaacaag taagtctacg cctttcttta ctgttaactt gcttgtatga
     3721 taaataactg ccaattcatc tcctgtagga agtttctcac caattttata ctctccattt
     3781 acaatttttt tctttatatc taagtaaatt tccatatact tcgcaatttt tttcaatggt
     3841 gaccctccaa tcaaaaacat atcttcatct atactttaac ataaataaac cgataaaatc
     3901 cacatcaaaa ttatacacac aataaaaaag atatatatat ttaaacaaaa ctatttactt
     3961 taagttgtaa gcggtgttat cattaagtgg aaacaggagg taaaaaaatt atgacaacta
     4021 tgaaaaagct aacttttcca gatgattttt ggtggggttc cgcttggtca gctgagcaag
     4081 cagaaggaaa aggagaaaca ggaaaagcag agactatctg ggatcgctgg tttaaagaac
     4141 aaccttaccg gttttatcaa cgaatcgatt cctctattac aaccgatcac atccatcaat
     4201 ataaagaaga tatagaacta atgaaaaaaa ctgggcataa ctcctttcgc gtttctattt
     4261 cttgggcacg aatgtttcca aatggcggta ccggaaaagt aaatcccaaa gccatttctt
     4321 tctatcgaaa attattttcc gaaatgaata ataacggaat caaagtattt gccaacttat
     4381 atcattttga catgcctgct actcttcaag ataaaggagg atgggaatca cgtgaagtag
     4441 ttgatgctta tgttcattac gctgaaactt gttttaaaga atttggtgac ttagtttgtc
     4501 attggtttac cttcaatgaa ccacttgggc ctattttagg tacttatctt gaagattttc
     4561 attatccaaa cctcattgac tttaaacgcg gagcgcaagc agcattcttc actatacttg
     4621 cacacgcaaa agcaatacaa gtatttaaag aactagctct tcctagtaaa ataggcgtta
     4681 ttctaaattt gagtcctact tttcctcgta gtcaaaatcc tgctgatgtg aaagcagcag
     4741 aaatcgctga tttattttat acgcggagct ttttagatcc tatggttaaa gggaattttt
     4801 ctaaaaagct tatcctgtta ttacaagaat ataatcagct tcctgaagat tatacgaaag
     4861 aagatcttgt atgtatctac gaaaataccg ctcaaattct tggactaaac tattacgagc
     4921 cacgtcgcgt caaatctcgt tcttctatgg tggatacatc tagtccattt ttaccagagt
     4981 ggtttttcga accacatatc atgcctggac gacgaatgaa cgaataccgg ggatgggaaa
     5041 tttacgaaaa aggaatttat gatttgtgca tggatatcaa gaaccattat ggaaacatcg
     5101 aagcatttat ctcggagaac ggaatgggcg ttgcggatga agaacgtttc atggatgata
     5161 cagggcaaat aattgatgat tatcggattg agtatatcaa agatcatctt gcttacttat
     5221 ggaaggcaat aaatgaagga tgtaacatta aggggtatca tctttggaca tttattgatt
     5281 gctggtcatg gatcaacgcc tataaaaacc gatatgggct ggtttcactc gaccttacta
     5341 ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa gcaattaagt gatacaaatg
     5401 gatttagata cgatgtagac ttgttagttg aataatattc taaaattcca cttgaagtga
     5461 acacattaga aaacaagaat aatgagaaat accaataaca aatacttttt cttcatactt
     5521 catttatact tgtcagtata agagactgtg acaaatgtag tgatcctcag aagttaggtc
     5581 tggaaatcta acttgagaag atttttttaa actacgtcca gtctttttta tttgtttaaa
     5641 attgaagttg acaagtcttc attaaaaatt tagaatattc acaagtttga cgacaattat
     5701 gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact
     5761 gcgatcaaag acacgttacc taccgttttt ggttatatcg gtattggact tgcatttggt
     5821 atcgttggga aagctgccgg gtttcatccg ctggtcgtca cgttgatgtc cctgctgatc
     5881 tatgctggtt ctgcccaatt tatcacggtc agcatgcttg ctagccatag tccactgctc
     5941 tccatcgttc tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt
     6001 gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca
     6061 gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt
     6121 cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt
     6181 ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc
     6241 gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa
     6301 cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt
     6361 atcccaagta acttagtgat tttagttgta acactgattg ggtgcggatt gggggtgtgg
     6421 atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac
     6481 ttggctttct aggatcctac catttgtctt actgaaaaaa ttcgatttgc cgcaaccgtt
     6541 gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct
     6601 atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt
     6661 tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggtaggaat
     6721 tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta tggcaaatta
     6781 ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg tattcttgct
     6841 tatccgttga acggaccact aacaacgact tttcctaata tatgtcctgt ttctacgtct
     6901 ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt gctcaatgta
     6961 ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc tccttgcgtt
     7021 tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa gctgatagat
     7081 tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt tcccacatgt
     7141 ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc gggtaatatc gaataataca
     7201 gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa gtcgtgatga
     7261 taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt cggacttgca
     7321 gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga accgacgccc
     7381 ccagctccgt tgatcactaa gatttttttc ccgcggttgg cattttcttc tggagtcaga
     7441 ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa ggctgcggct
     7501 tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac cagttggtat
     7561 tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa aaacgcgatc gccgacagaa
     7621 aatttttgca cttcatcacc tacagcaaca actttcccga ctccatcaaa ccctaagata
     7681 cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg attaacagat
     7741 actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg ttctttttca
     7801 tcaataaagc tttttggatc atcgatcgac aacccttcat aaaaaccgac taccttcatt
     7861 aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc atccatacag
     7921 ttttcatttc aatatatata gtatgctatt tgttccactt ttattcatca taattattct
     7981 cttacttaaa gtaaactatt tgcacttttt tggttaaaaa agaggttgcg acaagacttt
     8041 tgtcacaacc tcttttaaac ggtgttcaat caactgaccc tcggtattcg ctcaaaaaac
     8101 gacaaaatat gaggagctat ttttgtttgg ctgttcttat tacttgatgc aggacagctt
     8161 tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg aaaagcgttg
     8221 gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc atcagtagat ttcgtattca
     8281 tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt ttaaagaaac
     8341 gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc agatctagtt
     8401 gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcggttct ttttcaaatg
     8461 cataaggatt cttgaaaatc ccccggccaa tcatgatgcc atctacgcca tatttttcgg
     8521 ccaattcttc tcctgtctgg cgatctggga tatctccgtt gatcgtgatc agtgtttgcg
     8581 gagcgatttg gtcacgtaaa gcaatgattt tagggatgac ttcccaatgt gcatctactt
     8641 tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct tgatgaagta
     8701 ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc gtcttgacgc
     8761 tgacaggcaa accgccagct ttagccgctt caatcaattg tgccgctact tctgggcgaa
     8821 ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag cccatgttga
     8881 tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta aaaaattctg
     8941 gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaag gtcaatcttc
     9001 ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta aactcagtaa
     9061 agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca tctgttacat
     9121 cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca
     9181 tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc aaaaacattg
     9241 tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat gtttctgatt
     9301 gagcaaaaaa agactgtgac aaaaatctta tcacaattcc ttttccggaa taaatggtgt
     9361 tcaaaagctt gcatctgcgg taataag
//
LOCUS       DACECJ010000093         9362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.095, whole genome shotgun sequence.
ACCESSION   DACECJ010000093 DACECJ010000000
VERSION     DACECJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.095"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(93..761)
                     /locus_tag="IWO09_002441"
     CDS             complement(93..761)
                     /locus_tag="IWO09_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0265012.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="IWO09_002442"
     CDS             895..1182
                     /locus_tag="IWO09_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265013.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="IWO09_002443"
     CDS             1598..3136
                     /locus_tag="IWO09_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0265014.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="IWO09_002444"
     CDS             3133..4098
                     /locus_tag="IWO09_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0265015.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="IWO09_002445"
     CDS             4085..5407
                     /locus_tag="IWO09_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0265016.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="IWO09_002446"
     CDS             5648..6391
                     /locus_tag="IWO09_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0265017.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="IWO09_002447"
     CDS             6406..7398
                     /locus_tag="IWO09_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0265018.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="IWO09_002448"
     CDS             7410..8147
                     /locus_tag="IWO09_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0265019.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="IWO09_002449"
     CDS             8137..8859
                     /locus_tag="IWO09_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0265020.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9362)
                     /locus_tag="IWO09_002450"
     CDS             complement(8943..>9362)
                     /locus_tag="IWO09_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0265021.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3069 a         1607 c         1972 g         2714 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
     9361 ta
//
LOCUS       DACECJ010000094         9281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.096, whole genome shotgun sequence.
ACCESSION   DACECJ010000094 DACECJ010000000
VERSION     DACECJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.096"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..435
                     /locus_tag="IWO09_002451"
     CDS             <1..435
                     /locus_tag="IWO09_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0265022.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="IWO09_002452"
     tRNA            complement(759..842)
                     /locus_tag="IWO09_002452"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="IWO09_002453"
     CDS             1370..1870
                     /locus_tag="IWO09_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0265023.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="IWO09_002454"
     CDS             complement(2060..2404)
                     /locus_tag="IWO09_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0265024.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="IWO09_002455"
     CDS             complement(2558..3478)
                     /locus_tag="IWO09_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0265025.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="IWO09_002456"
     CDS             3665..4156
                     /locus_tag="IWO09_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0265026.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="IWO09_002457"
     CDS             complement(4180..6555)
                     /locus_tag="IWO09_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0265027.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="IWO09_002458"
     CDS             6881..7507
                     /locus_tag="IWO09_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0265028.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="IWO09_002459"
     CDS             7530..8234
                     /locus_tag="IWO09_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0265029.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="IWO09_002460"
     CDS             8426..9259
                     /locus_tag="IWO09_002460"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0265030.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DACECJ010000095         8696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.097, whole genome shotgun sequence.
ACCESSION   DACECJ010000095 DACECJ010000000
VERSION     DACECJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.097"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(4..216)
                     /locus_tag="IWO09_002461"
     CDS             complement(4..216)
                     /locus_tag="IWO09_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265031.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            403..870
                     /locus_tag="IWO09_002462"
     CDS             403..870
                     /locus_tag="IWO09_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265032.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            867..1109
                     /locus_tag="IWO09_002463"
     CDS             867..1109
                     /locus_tag="IWO09_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265033.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            1127..1429
                     /locus_tag="IWO09_002464"
     CDS             1127..1429
                     /locus_tag="IWO09_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265034.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            1500..3554
                     /locus_tag="IWO09_002465"
     CDS             1500..3554
                     /locus_tag="IWO09_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265035.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            3554..5386
                     /locus_tag="IWO09_002466"
     CDS             3554..5386
                     /locus_tag="IWO09_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ0265036.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            6081..7997
                     /gene="ltrA"
                     /locus_tag="IWO09_002467"
     CDS             6081..7997
                     /gene="ltrA"
                     /locus_tag="IWO09_002467"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0265037.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            8114..8696
                     /locus_tag="IWO09_002468"
                     /pseudo
     CDS             8114..8696
                     /locus_tag="IWO09_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
BASE COUNT         3274 a         1354 c         1745 g         2323 t
ORIGIN      
        1 attttatttg ttagttgaag tattaatatc aacgatttct tcagccaatt tatttaattt
       61 catagcattt atgaatctca tgttgtctat actagagcgt ttgttggcaa ggtcacttac
      121 tgtagttaag ggtacgccag attctatgga tatagaacga actgatttac cactatcaaa
      181 cagcaaccat tcgattttct ttttatcagc gtacatttca atgctccttc ttgttttttc
      241 tttattatag ctcttttttg taatgaatca caatatcaag agaaaaattt tctatagaaa
      301 ggtggatttt tatgagtatt cgttctccgt gtatccaaat gaggtttgtt gctactttcg
      361 ttatttgata tactaaaaat aaacaacgaa agaaggaata aaatgaagaa aaagcaacta
      421 tggtttgtct ctatgtttgc aatatttttt attattttaa gtggatgttc aagtaataag
      481 aaatcggaag ctaaagaaga taatagttta ttgcaaccag gaactgtttg gaaagaagaa
      541 gtaggtggtc ttattcataa tttaaaaatt atagatgata ctacctggga atattctgaa
      601 agtgtatggc atccagatcc tgtacaaata acagttaaga ggcaaaaaga ctatgaaggt
      661 ttagaaagat acaaaatagt agattctgca ggcattaggg aatttataaa caaacctgat
      721 tcattattta ttgttgttcc ctatgagaag gatggcgtaa aaaaaataat ttttcttgga
      781 tctagcaagg atgagaagcg aacgaaagag aaattaattc atgatggatc acaatcaaat
      841 aaatacaaat tacaaaagac tagcgagtga ttgctagtct ttttatttgt cctgaaggag
      901 gaatcacagt ggcaaaagct aaaacgccat ttgaaaaagc cgcgtctaaa atcttagaac
      961 gccgcggtgt atctgaaaca cagtggaaac aagaagtcct tgaaaaagca caatttgacc
     1021 tattacgggg aaaagacaag gacttagaag gatatatcta tcaacgtgaa cgtgaaaagc
     1081 ttgttttcga tgaaattatt aaacactaaa aggagcgaac aattttatgt ttgaatcaat
     1141 tacaatgaat tttttaacac aaccgaaagt agtagctgcg gttgatattt ttagagcatt
     1201 acaaactgcc gcaactagtt tgaaaaataa tctgatgatt tttgctgctg ttgcggcagt
     1261 ggcttccatt gctcttggag gtattttctt cttctttggg cgaagaggag ctgaaaacgg
     1321 gaagacacac gtaggttatg tattgttggg ggtaggagtt attttagctg ccgcttctat
     1381 tgtagtttca atgttctcaa tgtttggaca aaccccacaa ggactataaa aaaacaagta
     1441 gaaggagtaa aaagtacgtg gctacttttt actccttttt ttatggaagg agaatattca
     1501 tgttttttac gatttcaggc aatgcaaaat tggataaaga gttaataaga aaagcaacga
     1561 agaaagtggg aaagcgtact tcctttttac cgaaaacaat taaatattct gtcattgaaa
     1621 cggaagaaca gctacatgtg ttaggacaag tgttagaaca agagaatcaa tcttctgtta
     1681 aattgactct cacgttacat gataaagaag gaaaaataga aaaggctggc acagaatacg
     1741 tttttggctc actggattat caaatttcag aaggttttta tcctacattg attgatttaa
     1801 tccgcctaga tatatttaaa gatacggatg aaaccatgag tgtggaagag tcttatcgaa
     1861 acgaaaaaat tttacaagtt ttagaagaag agttagaaag cgaccaaaga aaagtgaatc
     1921 aagtgagtga agtcgaagca aacgaagaag taccatggga acgcccaatc tttccaaagg
     1981 atgaatcaaa agaaaaagag aaaggaatta ttgacgaaca agctgacatt ttgactcata
     2041 aatcaatatt tgaggaagag aacgaggtaa tgggtcaatt ggaggtagat ccctttgaag
     2101 aaacaatgga ggatgaagag acggccgaag tagaagttga tccagttgaa gctaatgaag
     2161 acccaacatg gactgaaggg gattctcagg aatatattga accacttatc agtgaagaag
     2221 tagagaaaaa tgaaacagag atacaaaagg caacggtatt tgattatatt ttggatcaat
     2281 atccgccaga gcatgaatgg atgaaagaaa aaaataaatt atttttaaat gaactttatt
     2341 cgaaatatca attacctcaa acctatcaag catttttaga gagtaaagaa gaattaatct
     2401 cacagggtaa aggcacactt ttagaacagt tagagtctgt ccaatcaaca aattggcatg
     2461 agcttgcaca agaagattta ttagctactt ttgaagaacg tcaacaagaa acagatcaag
     2521 aaattaatga gtatgcgacg gaacagtcgt cgaattggga gaaagcaaaa gaggaactaa
     2581 ataaagaaga acagcgagtg attgaagaag aagtacaaaa aattaaagag cggtacgaaa
     2641 agaaacgtga caatgcgtat tccttgtgtg tagaaaagat caacaatttt actcagacga
     2701 atcaagaagc attatcgaaa gaaaaagaaa acttattaaa agaacgtata aaagaacgaa
     2761 aagaagaatt ttaccgaggg ttgcgtactg ataagctaag gattagcaaa gaattaaatg
     2821 aacaactgaa taaactctat caaactacga ctgaagttct gatggaaaaa cacgagcaga
     2881 tccaacaaga attacaagca caaattccaa aatggaaaaa agagtatgaa attcaccaac
     2941 aagaattgaa agaacaagaa gaaaaacaac ggaaaaaaga aaaagagcgc gaacaattag
     3001 aactaaaaaa acgtgaacag gatcttgcgg aacaaaaaat ggcgatagaa aaacaacggc
     3061 tagaacaaga aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa caaatggata
     3121 cattgcgaca agcacagtta aattatctga cacatcctca accagtagct gctgttcaaa
     3181 cgccagtgaa caaccaagca aatttaactc cttcacccca gcgttccttt aacggatgga
     3241 tggtgggatg tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta
     3301 atcatatcaa tcaatcttca caggctgatt ctgtagccac aacggcgtca atcaaggaag
     3361 aagcccagga acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta
     3421 ccaatcagtc aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg
     3481 ctaaagaagg atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa
     3541 atgaggtgaa ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt
     3601 agaaaagaat aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc
     3661 agaaaaacat gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc
     3721 tttagagtat ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg
     3781 aagcttacaa ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga
     3841 ttcagctggt ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat
     3901 ttcgacagtt tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg
     3961 tcctctagca gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca
     4021 gatttatccg caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat
     4081 cacacattac aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg
     4141 tacctctcga tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg
     4201 tgcagaaaaa caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag
     4261 taaagatgaa ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact
     4321 gcaatcaatg agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat
     4381 agatactgca acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc
     4441 ttcagataac aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc
     4501 gttgttagaa caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt
     4561 tgagcttctt tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa
     4621 acagttgaat gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa
     4681 acaatatggg gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc
     4741 aatcacggga ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac
     4801 gattgattat aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct
     4861 tttaaatcaa cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg
     4921 tgtcaaagta ggctataaag gatttagtga aatcaatttt gattgtcatt tagttgcagg
     4981 tgatcaagtt tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt
     5041 ttctcaaaga gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga
     5101 aacccatttg ccagaattag aaaaagaagc cgttctaaca ttagatgagg aaaaaagtaa
     5161 cttattagta gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct
     5221 tacacctgat tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata
     5281 taaagaatta tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca
     5341 ttttatttta gatgaatttg ggaatagtgt gacctgttta gcctaaggat aaaagaggct
     5401 agttccaacg aacattggaa taactttact atcgagaaat caaatgacgg ggtaacgccc
     5461 tgaagggttt ccactgatac tgcgacatgc gttagtagga tgtgcatcca aacgcgtatg
     5521 aagctcgctg aagtcggcag aagggtaccg taaggaaaga cacgatacgt atctaccacg
     5581 caagtgctct agaggtaggg tatgtacctg atatgtcggg tgtcctaaaa agttcaataa
     5641 ggtgagaatg atttgattat ctgacaaact tgcgaatgga atgtctgaaa cgaataactc
     5701 atagaaatgt gagggggaag ggggatacgt cctctttcca ttgactcgag gttgagtaaa
     5761 aatgtgtttc tatgaaaaac cttgcatggt taaaggctat ggagtagtca acaggcaaaa
     5821 gcaagcacct aagttgaaat ctatggatag atagtaagga acaggggaag catctagcac
     5881 ggagggagca taccgaagat gtgtttctaa ggaaaaaccg aaatcggtta taacttaggt
     5941 gaatagatgt gaaagtagag ccacgaccag tgaaaatttc gtaatgaaat tggaggaaca
     6001 ggctcaagtc gttatttata atataagaaa cctatttaaa atcatggatt ctcgtaagac
     6061 taaaaaggtc acgaaccaga atggagcgtg ataaaaccat gactaacaca aaaggaatta
     6121 gcttaaagaa caaacaactc agacacgccg aatactacga catgactgag actttcgatg
     6181 aattgcacgc aatgagtgtt gcaaaatcaa attttaaaaa tctcatgaaa ttcattattg
     6241 atgacaataa tatctttcta gcgtatagaa atatcaaaaa gaataaagga agtgcaacgg
     6301 ccggatacga tggtgtcact ataaaagaca ttgaaaaatt ggcatgtggt gactttttaa
     6361 ctattattaa aaggcgattt aaggattaca gaccaagaaa agttaaaaga gtagagatcc
     6421 cgaaaccaaa cggaaaactt cgacctttag gaattccttc aatatgggac aggatagtcc
     6481 agcaatgtat tctacaaatt ttagaaccaa tatgcgaagc aaaatttaat aagcatagct
     6541 atggattcag accaaattgt tctacagaaa acgccatagc agattgtctt gctagaatca
     6601 atcaaagtaa gatgaattat gttgtagata ttgatataaa aggattcttt gacgaagtaa
     6661 accataccaa acttatgcgt caaatatgga caatggggat tcgagataaa caactccttg
     6721 taattattcg taagatgtta aaggctccga ttgttatgcg aaatggcaat gttgtttttc
     6781 caaccaaggg tactccacaa ggagggattt taagtccact gcttgcgaat atatgcctga
     6841 atgaatttga ttggtggata gctaatcaat gggaggaacg aactgtcaaa gagctgaaac
     6901 accactatcg aaaaaacgga gcatgggata aaggaaatat ctattcgaaa ttaagaagcc
     6961 acacaagttt aaaagagatg tatctggtac gctatgcaga tgattttaaa attttctgcc
     7021 gaacaggaga agaagcccaa agggcttttt atgccacaca aaaatggctc agagaaagat
     7081 taaagctccc aatttcaatg gagaaatcta aagttacgaa cctaaataaa gctgaaagtg
     7141 aattcttggg tttttcaata agactccaca aaaagggaaa aaagaaagtg gctttcacgt
     7201 acgtttgtcg caaagcagaa gaaaacatta aaaataaact aaaacaacag attaaaaaaa
     7261 ttcaatacgc tggagcaaaa aaaaataggc accgagcaat aggtgactac aacatgatgg
     7321 tcatcggtat ccacaattac tatcggatag cttcacattg tagtgtcaat ttcactgaga
     7381 tttatagaag tgttaggaca gtaatagaag caagactgaa agctagagga atgacgaaaa
     7441 atggaatcta tggacaagat catgaaggat accgtccgta tctaaagtct aagcaaatga
     7501 gatatattga aggttttccg atacttccaa ttggctatat taaaaaaaga actccactca
     7561 acaagcggag aaatctaaat aaatatacca aggaagggag aaagctcatt catcaagagc
     7621 aacaagctat agaaagctgg aaaatcaagt atcttgtaaa aggttttagt aacaataaga
     7681 aatacacggt ggaattttct gataacaaaa tatctaagtt tatttcccaa aaagggaaat
     7741 gcgctatcac aggaaacgaa cttttgatgg aagaaattga atgccatcac attaacccat
     7801 atcatttgtc caaagacgat agttacaaga accttgtcat tatctctaga gaaatccata
     7861 gattaatcca tgcaactagt gaagaaacta ttctgaaact gcttcggtta ctgaaactaa
     7921 atcactatca aattagaaaa ctaaacacgt atcgaaaaaa agcagctaat tttccaatat
     7981 caatcaaaat gaattagata ggaaacaaat tatattgtaa ttaacgatgg aacgccgtgt
     8041 gcggtgaaag tcgcacgcac ggtgtggacc gggggaaaag acggagataa tatcaaactc
     8101 ttacctatcg gaattgccac caatggataa tatggaagga atcatgacgg tcacagctgg
     8161 ccgaaacatg ttgtgggatc tgtttattca atcttatcag caactttatg cgaagtatgg
     8221 agaacaaaac gcagcgatta tcaaagaaaa ttgtcagaca catatttata ttatgtcgac
     8281 agatgataat accatcgaag aattttctaa aaaagtgggg aataagacgg ttgaacaaga
     8341 gaacagtacc atcaattcat taggtatgaa tactcatatc aatcgcaatg ttgattcaga
     8401 tcgtatctta actcctgaaa gaatttctac ttttttagaa ggtgaaacga ttgtactcgc
     8461 tcctttaaaa cgacaagatt tgaaaggtca taaaatacgg ccgtatccta tttttaatca
     8521 tggggaaacg aatcttcctt atgcttatca atatattgaa gatttcaatc catcgtggga
     8581 tatcaatgat atagacatac attgtacaca tgcggacttg gatcttaagg ctttacaaat
     8641 tgattatgat tatttcctac cagatgaata tgctagaaat ctctatcatg gactgc
//
LOCUS       DACECJ010000096         8493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.098, whole genome shotgun sequence.
ACCESSION   DACECJ010000096 DACECJ010000000
VERSION     DACECJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.098"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWO09_002469"
     CDS             complement(<1..173)
                     /locus_tag="IWO09_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0265038.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWO09_002470"
     CDS             complement(593..1714)
                     /locus_tag="IWO09_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ0265039.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWO09_002471"
     CDS             2042..3451
                     /locus_tag="IWO09_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0265040.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWO09_002472"
     CDS             3448..4176
                     /locus_tag="IWO09_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0265041.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWO09_002473"
     CDS             complement(4304..5215)
                     /locus_tag="IWO09_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0265042.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWO09_002474"
     CDS             complement(5232..6239)
                     /locus_tag="IWO09_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0265043.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWO09_002475"
     CDS             complement(6236..8365)
                     /locus_tag="IWO09_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0265044.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWO09_002476"
     CDS             complement(8370..8477)
                     /locus_tag="IWO09_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265045.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACECJ010000097         8293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.099, whole genome shotgun sequence.
ACCESSION   DACECJ010000097 DACECJ010000000
VERSION     DACECJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.099"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(48..1514)
                     /locus_tag="IWO09_002477"
     CDS             complement(48..1514)
                     /locus_tag="IWO09_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0265046.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="IWO09_002478"
     CDS             1901..2497
                     /locus_tag="IWO09_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0265047.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="IWO09_002479"
     CDS             complement(2555..3037)
                     /locus_tag="IWO09_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265048.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="IWO09_002480"
     CDS             complement(3143..3340)
                     /locus_tag="IWO09_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265049.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="IWO09_002481"
     CDS             3635..4393
                     /locus_tag="IWO09_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0265050.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="IWO09_002482"
     CDS             4453..4881
                     /locus_tag="IWO09_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265051.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="IWO09_002483"
     tRNA            4927..4999
                     /locus_tag="IWO09_002483"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="IWO09_002484"
     CDS             complement(5149..5520)
                     /locus_tag="IWO09_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0265052.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="IWO09_002485"
     CDS             5735..5920
                     /locus_tag="IWO09_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265053.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="IWO09_002486"
     CDS             complement(6003..6191)
                     /locus_tag="IWO09_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265054.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="IWO09_002487"
     CDS             complement(6215..6478)
                     /locus_tag="IWO09_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265055.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="IWO09_002488"
     CDS             6636..6893
                     /locus_tag="IWO09_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0265056.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="IWO09_002489"
     CDS             complement(6964..7155)
                     /locus_tag="IWO09_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265057.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="IWO09_002490"
     CDS             complement(7296..7616)
                     /locus_tag="IWO09_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265058.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="IWO09_002491"
     CDS             complement(7639..8073)
                     /locus_tag="IWO09_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0265059.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2630 a         1636 c         1314 g         2713 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acg
//
LOCUS       DACECJ010000098         7761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.100, whole genome shotgun sequence.
ACCESSION   DACECJ010000098 DACECJ010000000
VERSION     DACECJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.100"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            147..1094
                     /locus_tag="IWO09_002492"
     CDS             147..1094
                     /locus_tag="IWO09_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0265060.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="IWO09_002493"
     CDS             1106..2026
                     /locus_tag="IWO09_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0265061.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="IWO09_002494"
     CDS             2067..3524
                     /locus_tag="IWO09_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0265062.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..4483
                     /locus_tag="IWO09_002495"
     CDS             3869..4483
                     /locus_tag="IWO09_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0265063.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4476..6200
                     /locus_tag="IWO09_002496"
     CDS             4476..6200
                     /locus_tag="IWO09_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0265064.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6197..7648
                     /locus_tag="IWO09_002497"
     CDS             6197..7648
                     /locus_tag="IWO09_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0265065.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2617 a         1291 c         1544 g         2309 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     4201 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     4261 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     4321 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4381 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4441 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4501 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4561 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4621 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4681 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4741 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4801 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4861 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4921 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4981 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     5041 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     5101 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     5161 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     5221 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     5281 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     5341 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5401 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5461 agacgcggat cgacataaca aacacggaat atgaactgag ggaactttca atcggtatca
     5521 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5581 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5641 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5701 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5761 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5821 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5881 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5941 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     6001 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     6061 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     6121 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     6181 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     6241 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     6301 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6361 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6421 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6481 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6541 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6601 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6661 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6721 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6781 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6841 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6901 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6961 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     7021 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     7081 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     7141 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     7201 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     7261 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     7321 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7381 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7441 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7501 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7561 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7621 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
     7681 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7741 tcatgacctc tcgataaacg g
//
LOCUS       DACECJ010000099         7629 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.102, whole genome shotgun sequence.
ACCESSION   DACECJ010000099 DACECJ010000000
VERSION     DACECJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.102"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(60..788)
                     /locus_tag="IWO09_002498"
     CDS             complement(60..788)
                     /locus_tag="IWO09_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0265066.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(801..2081)
                     /locus_tag="IWO09_002499"
     CDS             complement(801..2081)
                     /locus_tag="IWO09_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0265067.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2222..2824)
                     /locus_tag="IWO09_002500"
     CDS             complement(2222..2824)
                     /locus_tag="IWO09_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0265068.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3069..3275
                     /locus_tag="IWO09_002501"
     CDS             3069..3275
                     /locus_tag="IWO09_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265069.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3587..4306
                     /locus_tag="IWO09_002502"
     CDS             3587..4306
                     /locus_tag="IWO09_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0265070.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4387..5070)
                     /locus_tag="IWO09_002503"
     CDS             complement(4387..5070)
                     /locus_tag="IWO09_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0265071.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5288..7474
                     /locus_tag="IWO09_002504"
     CDS             5288..7474
                     /locus_tag="IWO09_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0265072.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2733 a         1284 c         1438 g         2174 t
ORIGIN      
        1 aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt
       61 tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac
      121 gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat
      181 ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat
      241 tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa
      301 aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag
      361 atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg
      421 acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg
      481 ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa
      541 ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt
      601 tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt
      661 tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta
      721 tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg
      781 ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg
      841 ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata
      901 aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac
      961 tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc
     1021 ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca
     1081 ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg
     1141 attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt
     1201 aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc
     1261 ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat
     1321 ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca
     1381 tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc
     1441 atcctgactt gttgctctaa taactgatca gctaatttct tattagagat tttccaataa
     1501 taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct
     1561 aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat
     1621 aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct
     1681 aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga
     1741 aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgtcg
     1801 atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc
     1861 aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt
     1921 agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga
     1981 atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt
     2041 gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga
     2101 tttttaccaa attatagcac aattagcgta ttattttttt aatttttcag ggattttgaa
     2161 ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg
     2221 tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag
     2281 gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag
     2341 ctgagaaaaa taaagtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata
     2401 atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa
     2461 gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt
     2521 gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa
     2581 cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa
     2641 aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta
     2701 gttttccaag attattcaat aaaacttcta aggcaaattt tacttggaca tacaccatat
     2761 cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt
     2821 tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg
     2881 tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt
     2941 tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag
     3001 tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag
     3061 ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg
     3121 atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga
     3181 tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt
     3241 tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata
     3301 aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct
     3361 tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt
     3421 tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac
     3481 ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc
     3541 tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt
     3601 gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca
     3661 agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt
     3721 ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt
     3781 tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga
     3841 agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa
     3901 gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa
     3961 gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa
     4021 aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa
     4081 agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt
     4141 gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca
     4201 agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta
     4261 cactacaaat aacccgaaag caaagggaat ctttacaaat aaataatata aaaactaagc
     4321 aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca
     4381 ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt
     4441 gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg
     4501 atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg
     4561 gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc
     4621 tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc
     4681 aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg
     4741 ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa
     4801 caagcgccag aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag
     4861 aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc
     4921 gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc
     4981 atattggata taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat
     5041 tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg
     5101 tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc
     5161 atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac
     5221 gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat
     5281 caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa
     5341 caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga
     5401 agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga
     5461 aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga
     5521 acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg
     5581 gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt
     5641 agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa
     5701 acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat
     5761 cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat
     5821 acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat
     5881 acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt
     5941 agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa
     6001 atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct
     6061 cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca
     6121 aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga
     6181 ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg
     6241 gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta
     6301 tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa
     6361 aactcttttc ttattgacta attatcgttc tcgggcagct attgtccaag gagcaaatag
     6421 attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga
     6481 agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat
     6541 caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata
     6601 ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt
     6661 tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt
     6721 ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta
     6781 ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca
     6841 atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc
     6901 caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga
     6961 ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa
     7021 ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt
     7081 cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg
     7141 cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag
     7201 taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta
     7261 taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtgac
     7321 gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct
     7381 gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa
     7441 agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg
     7501 tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt
     7561 cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga
     7621 caagaattt
//
LOCUS       DACECJ010000100         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.103, whole genome shotgun sequence.
ACCESSION   DACECJ010000100 DACECJ010000000
VERSION     DACECJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.103"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWO09_002505"
     CDS             complement(138..428)
                     /locus_tag="IWO09_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265073.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWO09_002506"
     CDS             complement(546..806)
                     /locus_tag="IWO09_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265074.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWO09_002507"
     CDS             complement(943..1356)
                     /locus_tag="IWO09_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0265075.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWO09_002508"
     CDS             complement(1812..2297)
                     /locus_tag="IWO09_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265076.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWO09_002509"
     CDS             complement(2436..2651)
                     /locus_tag="IWO09_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0265077.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWO09_002510"
     CDS             2875..3675
                     /locus_tag="IWO09_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0265078.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWO09_002511"
     CDS             3694..5562
                     /locus_tag="IWO09_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0265079.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IWO09_002512"
     CDS             5555..6046
                     /locus_tag="IWO09_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0265080.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IWO09_002513"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IWO09_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0265081.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IWO09_002514"
     CDS             6606..>7600
                     /locus_tag="IWO09_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0265082.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACECJ010000101         7553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.104, whole genome shotgun sequence.
ACCESSION   DACECJ010000101 DACECJ010000000
VERSION     DACECJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.104"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(571..1917)
                     /gene="gor"
                     /locus_tag="IWO09_002515"
     CDS             complement(571..1917)
                     /gene="gor"
                     /locus_tag="IWO09_002515"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0265083.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2051..3292)
                     /locus_tag="IWO09_002516"
     CDS             complement(2051..3292)
                     /locus_tag="IWO09_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0265084.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3310..4233)
                     /locus_tag="IWO09_002517"
     CDS             complement(3310..4233)
                     /locus_tag="IWO09_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0265085.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4454..6946)
                     /locus_tag="IWO09_002518"
     CDS             complement(4454..6946)
                     /locus_tag="IWO09_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0265086.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6953..7420)
                     /locus_tag="IWO09_002519"
     CDS             complement(6953..7420)
                     /locus_tag="IWO09_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0265087.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2079 a         1641 c         1213 g         2620 t
ORIGIN      
        1 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
       61 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
      121 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
      181 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
      241 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
      301 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
      361 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
      421 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
      481 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
      541 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
      601 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
      661 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
      721 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
      781 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
      841 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
      901 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
      961 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     1021 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     1081 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     1141 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     1201 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     1261 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     1321 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     1381 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     1441 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     1501 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     1561 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     1621 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     1681 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     1741 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     1801 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     1861 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     1921 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     1981 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     2041 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     2101 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     2161 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     2221 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     2281 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     2341 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     2401 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     2461 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     2521 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     2581 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     2641 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     2701 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     2761 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     2821 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     2881 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     2941 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     3001 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     3061 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     3121 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     3181 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     3241 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     3301 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     3361 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     3421 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     3481 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     3541 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     3601 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     3661 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     3721 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     3781 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     3841 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     3901 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     3961 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     4021 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     4081 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     4141 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     4201 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     4261 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     4321 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     4381 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     4441 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     4501 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     4561 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     4621 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     4681 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     4741 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     4801 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     4861 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     4921 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     4981 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     5041 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     5101 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     5161 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     5221 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     5281 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     5341 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     5401 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     5461 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     5521 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     5581 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     5641 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     5701 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     5761 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     5821 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     5881 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     5941 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     6001 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     6061 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     6121 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     6181 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     6241 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     6301 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     6361 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     6421 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     6481 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     6541 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     6601 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     6661 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     6721 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     6781 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     6841 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     6901 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     6961 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     7021 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     7081 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     7141 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     7201 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     7261 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     7321 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     7381 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     7441 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     7501 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atc
//
LOCUS       DACECJ010000102         7534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.105, whole genome shotgun sequence.
ACCESSION   DACECJ010000102 DACECJ010000000
VERSION     DACECJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.105"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..179)
                     /locus_tag="IWO09_002520"
     CDS             complement(<1..179)
                     /locus_tag="IWO09_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0265088.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="IWO09_002521"
     CDS             complement(192..1295)
                     /locus_tag="IWO09_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0265089.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="IWO09_002522"
     CDS             complement(1385..2320)
                     /locus_tag="IWO09_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0265090.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="IWO09_002523"
     CDS             2622..4556
                     /locus_tag="IWO09_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0265091.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="IWO09_002524"
     CDS             5013..5429
                     /locus_tag="IWO09_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0265092.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="IWO09_002525"
     CDS             5443..6009
                     /locus_tag="IWO09_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0265093.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="IWO09_002526"
     CDS             6032..7018
                     /locus_tag="IWO09_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0265094.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7534)
                     /locus_tag="IWO09_002527"
     CDS             complement(7173..>7534)
                     /locus_tag="IWO09_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0265095.1"
                     /translation="SDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSST
                     TSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVIL
                     LVIGLRITFMSYKKSKR"
BASE COUNT         2172 a         1443 c         1546 g         2373 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagc
//
LOCUS       DACECJ010000103         7476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.106, whole genome shotgun sequence.
ACCESSION   DACECJ010000103 DACECJ010000000
VERSION     DACECJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.106"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(81..416)
                     /locus_tag="IWO09_002528"
     CDS             complement(81..416)
                     /locus_tag="IWO09_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0265096.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(400..672)
                     /locus_tag="IWO09_002529"
     CDS             complement(400..672)
                     /locus_tag="IWO09_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ0265097.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1123..1926)
                     /locus_tag="IWO09_002530"
     CDS             complement(1123..1926)
                     /locus_tag="IWO09_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0265098.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1904..3028)
                     /locus_tag="IWO09_002531"
     CDS             complement(1904..3028)
                     /locus_tag="IWO09_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0265099.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(3044..3859)
                     /locus_tag="IWO09_002532"
     CDS             complement(3044..3859)
                     /locus_tag="IWO09_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0265100.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3849..4730)
                     /locus_tag="IWO09_002533"
     CDS             complement(3849..4730)
                     /locus_tag="IWO09_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0265101.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(4806..6095)
                     /locus_tag="IWO09_002534"
     CDS             complement(4806..6095)
                     /locus_tag="IWO09_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0265102.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6323..6967)
                     /locus_tag="IWO09_002535"
                     /pseudo
     CDS             complement(6323..6967)
                     /locus_tag="IWO09_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2296 a         1606 c         1147 g         2427 t
ORIGIN      
        1 atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt attgacaaag
       61 agcccttctt tttagtatta ttataattct tccatgcgtt cttccagtcg ctgtaaatcg
      121 cttggactta attccccaat ttttttccat ttgacctttt tgttgttttt gggtaattcg
      181 tttagagttc ctacgtcaat ccaagattct ttttttagtc ctgcctgttt ccaatccctt
      241 atttcaacgt atttcagctt tattttatcg gatttagtat gatattttga agtaattgac
      301 agaaacgaaa ttgtgtccag tccatttttt agaatataga caggccggcg tttaccgccc
      361 tctgcagctg tgaaagcaac gtagacggtt gctatatcat tagtattcat cttcattgaa
      421 ccattcttca attttttcgt cagtgtctag tttttggaca ggcaattttt ccgctaatct
      481 ttgaatatta tggattcgtt tacttttttc cgacaattca atagaaaatg ggatttttcc
      541 ttgctcaaca atttgcttat aaaaaacggt gacggctgtt ttggggctca tgcctagttc
      601 atcaataatg ctttctgctt catttttcaa tttttcgtcc atagaaattt gtaacttagc
      661 cattttctcc acctctttct gacaaacaca ttatatcacg gaaagagttc aaatggaatt
      721 aatacaacta cttttgaact ccttgaaatg cagtgtattt atctaaatat aaggaaagga
      781 tatagtgcta aatggccttg atagatagaa aagtttcaaa atccgccaca gcgataattg
      841 aagcgaacac tcaacgacac acagaacagg aaaagactcc taaaagaacc aaattaacta
      901 ggacgtgtct gaaaactcca caaagatgtg gtatgctctg gtaaaaaacg aatggcacaa
      961 ccacgaagac cgatatggtt cttggaaaac tttctccacc ctttttgtaa ttggagggat
     1021 agtggtttat tgatagcgat tttccaagtt cttcaagtgg aaccagacat tgagcaccac
     1081 ggctaaaaag tgaatgaagt ctttattttt aatcaataat ttttaatgat taccagtaat
     1141 tggttttttc atgcgcccta tttctatttg atgtggagtt aaataatgct gataagtaaa
     1201 aatttttccg tcaattaaac tgccactagt gaatcctgtt ctattggtaa aaagagaata
     1261 gtctgggtat tcatctacac caaatgataa agattcagcc ataaactgtt ttttaccagc
     1321 aaattcagca ctgctacctt gatggatatg tcctacaaaa atacctaaaa tatcactttc
     1381 ggcaatcaat tcagcaaaac cttccggaac ttcagttaca atagatggat cttcccacga
     1441 caatggatga tgaaccaata aaatcgttcc cttttcagca ggtgtaccca accactcttg
     1501 taattgaatc atctgtccta tcgatatttt tccttcgtgc tgggtatctt gagcagaatc
     1561 caaggaaata attcgtaacc cattttgatt cgtacaatgg atatactctc ccgtttcatt
     1621 gctttcaaca ctcattcctt caaagaaaac actgcgacga tcatgattcc cacgacaaaa
     1681 atagtaaggt gtggttggga aatattgatc caacattttc cgtaagactt gataatcaga
     1741 aatcccccct tcatgtacta aatcacctgt taatagaata aaatcgtaca ctgttaacga
     1801 gagttcttgt aacaattttt ccaatgccaa ctgtggattt ttaattgttg gaaatagttc
     1861 agcagtaaac aagttagttt gatagttttc tctaaaatgg atatcagaga gatgcaaaaa
     1921 gcgcatggct attccctctt tcttcaataa aataatcagg atatcctaaa ttttcttgcg
     1981 tctgataatc aaaaaaatgc attctttcag gttctatgcg gagattgact gcatcaccaa
     2041 tctgccaagt aggtttttca ttaaggacaa tcatttctat accatcgact ttaacggtgt
     2101 acgcataact ttgacctaat agctcacgat acttcacttg accgtgtaaa tcgcttgttt
     2161 cttttgaaaa ggttacgtgt tctggtctta tgcctaaata caatgagcca ctaacctgaa
     2221 gaagttgttt ttgccaagct ggattcaatg ttaaaaattg actggctacc tttaatgttc
     2281 cttgatcata agtaactggc ataatattca tgcctggcga accaataaaa tttgcagtaa
     2341 aagtattttt aggtttcgta taaacatttt tcggcgtatc aatcatctga attctacctt
     2401 cattcaataa aacaatccgg tcagctaacg tcatcgcttc aatttgatca tgagtgacgt
     2461 agacaaaagt ttgtttataa cgttgatgaa tttttaccaa ttcttttcgt gcatccaatc
     2521 gtaaacgcac atccaaattt gataacggtt catctaataa aaaataatta ctccgtttaa
     2581 ccaccgcacg agccaatgct acacgctgcc gttgccctcc tgacaaatct ttaggaagtc
     2641 gatcttggta tggcgttaaa cctaacatct tcaaagcttc ttccaaacgg tgctgaatgt
     2701 cagctgtctt aattttattc gcccttaatg caaaaataat attctccttt acagtcatgt
     2761 gaggatataa agcataattt tgaaaaacca ttgcgatctc tcgttctcca caaggtagct
     2821 tatttgctag ttgtgtacct aaaaaaagct ctccactcgt aattttttcc aaaccagcaa
     2881 tcattctcag caaagtagat tttcctgaac cagatggtcc cagtaacaca attcgttctc
     2941 cagcggccac agctaaatca atttctttta aaatggtatg ttctccatat ttttttccga
     3001 tatttcttaa ttcaagcgtt tctcccatga tttgtcctcc aatttattta atacctgcag
     3061 aaacaaaagt cccgataata tatttttgaa aaattgcata caagactaac ggtacaaaca
     3121 ttgtaattaa actaatcgcc attgctacgg taaaatttgt cccaccttca gaactaatat
     3181 aggtctgcat cgccaacggc aaagtataat tatctactgt ccgtaaaatt aataccggcc
     3241 aaacatactc attccaagag gtaataaaaa accaaatacc ggtagaagta atcgcatgtc
     3301 tagtaagagg aaaaacaatt ttactcatga cggttgtttg tttgatgtta tccaatgccg
     3361 ccacatctag aaggctcact ggaatttccc gcattgtttg cgtcaataaa aaaatgccca
     3421 tcgcatcagc aaattgtggt attatcacac cccaagtagt atcaattaaa ttaagttttg
     3481 caatcaacaa ataattagga atcatcgcaa cagtaaaagg aatgaacatc gtaccaatga
     3541 tgataaagta ccagattttc ttccctttga ttcggcaata agtaaaacta tatgccgcaa
     3601 aaaaagctac gcctaactta acaaccgtta taattgtagc aataaaaaaa gtatttctcg
     3661 taattttgat aattggaaga ttttcgaaca aatagcaaaa attttccaag gtaaacggct
     3721 gtggtatcaa actagtaatc gtctgatagg catctttcaa tggcttaaaa gaattggcca
     3781 taataaacaa taatggatag ataataatga ttatgatgaa cataaataag agatgtggca
     3841 accagcgttt aattttcata gtaaaccccc ttttccacaa atttaaatat tacccctaag
     3901 aaaacagcaa atattaataa agttaaaact gaaattgctg aagattctcc gactttatac
     3961 atttcaaaag ccttttgata agcataatag atcacgtttg cactaccatt attcggtcca
     4021 ccacgagtga tgatcttaat cggagtaaac acatactgca gaccttgaac aatggtcatc
     4081 accaataaat atagtcctgt cgaactactc ataggcaaca caattgtaaa aaaaatgcgc
     4141 cagatgggta cacgatccaa cttagctgct tcgatcatct cttgatcaat tcccacaatt
     4201 gctgcaaaaa tgacaatgaa attataacca aaaactttcc aagcagtaat acctgcaatc
     4261 acagcaatta cccaaccttg taaattcatc cacataggca gtcttaaacc aaaatttccc
     4321 aacaagacag caattggtcc cgataccgga tttaatatcc atgtaaaaac catccccgcc
     4381 actaccaaag aaatcacagc tggaataaaa aataatggtt tatacaaatt ctgccctttt
     4441 tctataacta ttcctaataa aaaggatgta atatatggga acacacagct cagcaagaca
     4501 aatagaacgg tataggacaa agtattcttc aacactttat gaaagactgg atcagtcagc
     4561 acctttacat agttggcaaa gccaacaaat tctttagtgg gggccaccat attccagtca
     4621 aaaaaactca aatagatcgt ataaatcaat ggataaaaaa caaacacgcc caacaaaata
     4681 attgctggcg ccagatacaa ataacctaca tagcgcttca tttccgacag cctcctcatc
     4741 cttatgtcaa aagcagacga ccttgttttt cctttcttac aggcgctgtc gtctgcttgc
     4801 tttgcttatt tgagtaactc attaatttcc ttttgagtag ctttcatcgt ttcattgacg
     4861 gaattacctg ataaaatttt atcccgcata tccaacaatt tttgttcagc ttctaatcca
     4921 gcatctccag gaaatgaact ccaactgacc atcttatcca tctgatcaat tgctggtatc
     4981 atcatctgat tttcagctaa gaaagctttt aatccatttt gggcgtctgc aacatcttta
     5041 cgtggtggta aataaccagt accttctgtc catttagcta aagattcaac actataaaga
     5101 tattttaaga aatcccaagt tgcttccttt ttcttatccg tttgtgatgt aacagctaac
     5161 attgcgccac cagcaggtag tttaactttt ttcccttccc aacttggtga ctcaactgct
     5221 ttagcagtaa aagtactatt tttttgaatg ttgtcccttt gagcaatagt ggtatagacc
     5281 atcgcgacgt taccatcaat aaagctttgc acaccttgtt cccacgtagt gtgtaatgcc
     5341 gtctggtctt ttaccatcct ttgatataat tcataagcag cgataccatc tttagaagca
     5401 aaggtagctt tgccatcttt tattacactt gccccattac tttctaaaat tgcttgttgt
     5461 gcccaattat cagcaggttc ttgaatatac acgccatact ttccagtttt ttcttttatt
     5521 atcttagaaa aagtttgaac ttcttcccaa gtttttggac cattttcatc caagcccgct
     5581 gcttttaata tatcttgatt taaatataaa accggattac taatggagta aggaataccc
     5641 acttgtttac catctttttc agacagtgcc atgatattgt ctaaaaaatg atctttaaaa
     5701 taatcagcat tatccggatc cacttcttct actaaagatt gaggattagt atatttgaaa
     5761 ttttctgcaa aataatcttt aaaagcccat cccacttgta ctacgtccgg tatttctttg
     5821 gaggcaacag aagcttgtaa attttgcatc aagcctttat acatatcagg attatatttt
     5881 gctacaacct tcactttgtc ttgagattga ttgtatttat ctactaattc attcactgtt
     5941 tttccaccta gggtatccgc attaacatgc caatactcta tcgttacacg accctccgat
     6001 ttttttgttg tcttggtatt cccactacca caagctgcca aagagatgac acttaaaaca
     6061 gacacaaccc ctataacaag atttttcttt aacataacat taacttcctc ctaatttctt
     6121 tatggccatt tttctaatca agaaatatta tgaactacca atgtaaattt ctagttaatt
     6181 ttctgtaaat aattcgaaat ttgtgcattt cggggtacag ggtcactgga catatccatc
     6241 aagctaagaa taaagccccc atatggtcat gtatcccatc tccaaatctc ttaactcctc
     6301 tgttttgatt ataaaatagt ttattctgtg ctcctttctt ccataaatag gcccatgcac
     6361 aacgtgaggt tatattataa acaccacagc gcgcaaggta ctcaatcgtt tctagttcat
     6421 tgactgtcca tacagaaaat gtcaatccta gctgctgccc cacttcacgg gccaacttag
     6481 ttaaccatcc ttcatgaata ttgacgattt gaacgctttg ataggctaat ttctgtaagg
     6541 tatcgatcaa atctgcttcg gtaatatccc caagtttttt cccttcgaac tgattttcaa
     6601 tattataaaa tatatgcttt ccccaaatcg cagcccaaga taattgtaca tagccactaa
     6661 gaacaatttg tggccagatt tgataggttt gtgccagcga aaaaacggcc atttccaatc
     6721 cctcttcttt taaatcacag ttaatggtca tggttgcctt ctcattggcc actgcttgaa
     6781 acacctgctc aagggttacc gaatctggga caacttctta cctgtcatgg gctagtatta
     6841 gcgtaccatc tgtttgctga cgcacatcta cttcaaaaca aagctggcga tccaaacaaa
     6901 agcgaagaaa tgcccagcta ttctcctccg tattgtcact accagagtgt gcagtgatca
     6961 tggtcatttt gacccctcct ttaattccgt gattttctcc cacacgtcca ctataccgat
     7021 gaagcgttaa tttcttgtta atttcttgta aattccctgc gttttttcag taaactagtt
     7081 gtaaatcttg catgctcctt cctacgaact catgaatcgt cgattttgcc cttctagcta
     7141 gtttcaccaa ttaagtagtt ctacattccc cgcttttaac ctactacacc tttttttgtt
     7201 ttcagctatt ccttcttctt gctgaaagtc cttgaggttc tttcttacag gataccatca
     7261 aggtgttcaa tggcttattc ccattgaatg accgagactt actatcagat ttctgctcgt
     7321 tgaaccaagt ctttgataca ggatttcttc cgtcatcttc tcacaaatac caccttgcgt
     7381 ttctctagcg attggtcgat gtgcaccctc ccagtggact gtcactaccc ctttttgcta
     7441 tgcctagccc acaaaaaaag ctgtcctcac agacag
//
LOCUS       DACECJ010000104         7418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.107, whole genome shotgun sequence.
ACCESSION   DACECJ010000104 DACECJ010000000
VERSION     DACECJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.107"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IWO09_002536"
     CDS             complement(<1..703)
                     /locus_tag="IWO09_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0265103.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IWO09_002537"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IWO09_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IWO09_002538"
     CDS             1758..2837
                     /locus_tag="IWO09_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0265104.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IWO09_002539"
     CDS             2869..3462
                     /locus_tag="IWO09_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0265105.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IWO09_002540"
     CDS             complement(3572..3799)
                     /locus_tag="IWO09_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0265106.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IWO09_002541"
     CDS             complement(3902..6490)
                     /locus_tag="IWO09_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0265107.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IWO09_002542"
     CDS             complement(6510..7169)
                     /locus_tag="IWO09_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0265108.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..>7418)
                     /locus_tag="IWO09_002543"
     CDS             complement(7218..>7418)
                     /locus_tag="IWO09_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0265109.1"
                     /translation="GKETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYE
                     ALRQQGFPNLELTHHYENDKLD"
BASE COUNT         2046 a         1643 c         1324 g         2405 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttcc
//
LOCUS       DACECJ010000105         7327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.108, whole genome shotgun sequence.
ACCESSION   DACECJ010000105 DACECJ010000000
VERSION     DACECJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.108"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            95..547
                     /locus_tag="IWO09_002544"
     CDS             95..547
                     /locus_tag="IWO09_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265110.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            561..1250
                     /locus_tag="IWO09_002545"
     CDS             561..1250
                     /locus_tag="IWO09_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265111.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1375..3726
                     /locus_tag="IWO09_002546"
     CDS             1375..3726
                     /locus_tag="IWO09_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0265112.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGAYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3806..4201
                     /locus_tag="IWO09_002547"
     CDS             3806..4201
                     /locus_tag="IWO09_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265113.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4526..5143
                     /locus_tag="IWO09_002548"
     CDS             4526..5143
                     /locus_tag="IWO09_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0265114.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5184..5483
                     /locus_tag="IWO09_002549"
     CDS             5184..5483
                     /locus_tag="IWO09_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265115.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5517..5684
                     /locus_tag="IWO09_002550"
     CDS             5517..5684
                     /locus_tag="IWO09_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0265116.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5729..6334)
                     /locus_tag="IWO09_002551"
     CDS             complement(5729..6334)
                     /locus_tag="IWO09_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0265117.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6594..7274)
                     /locus_tag="IWO09_002552"
                     /pseudo
     CDS             complement(6594..7274)
                     /locus_tag="IWO09_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         2476 a         1063 c         1422 g         2366 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa ttatcttatc gtagatatat
       61 attataaatt taataagaaa gcgaggtgag aatcgtgaaa aaaacgatac taagtatttt
      121 ttcagtattt acaatattaa ttgggggcta ttttcttttg aatttagatt ctgttaattc
      181 aaacgctcaa ataaaagatc aaagagtagc tgaacataat aataaaaaaa tgagtttgac
      241 caaaaatatc tattctatgt cagatgaaga attgtataaa gaatttggtg atttatccgg
      301 ggaacagtta gaagaaagta ttccaggaat tagtgactca gataaaattg ttgtcttgtc
      361 tgctggtggg caattaatgg gagatggcaa atccgaatta gttagttata aacaaaatca
      421 cagacagttt attgatgtta atactgattc caacgctatt tcgattaagg atctaaaaga
      481 gcttttaaat agtaaaagaa atgaattaaa gcagataaaa gaaactaatt cagtggaatt
      541 agagtaaagg aagattcatt atgaaaaaga tttttggtgt ttctgttggg ctacttatga
      601 tgttaacatt tggaaatgca gtatatgctg ctgagaaaga agttatttca gatccagagc
      661 agtacgcctc acgatacttt gctgattttg aagaagaaga taaagtactt gcattgccag
      721 aaggtggttt tttaaaaggg gaagctaaaa ttgtatctat ggataatcca gatataattc
      781 aaggttcata taattcagaa actgatcctg cttcagtttc agtgacagaa gcaaaaaaaa
      841 ttgttgctga aaaattcaaa aatgatattg aaactactcc aattatgcct cgaggagcat
      901 ttccacccga tgttggtact agcggacacc ttcttgtact agcaggagga ggaagctata
      961 aatcacagcc atttagtggt gcgggatggc gttttggagg tatgggtgta aaaccagctg
     1021 atggtacagg caacttttta cgttggaaat cctacattga cgatggaaga gtaggaaatg
     1081 taaatcaagc caacgcaact aaatctggtt ctattcaagg aatggcattg tatgctcgtg
     1141 aaggattcaa atactttgca ccaaacgggc ctggttggac aatgaatata ggtatttatt
     1201 acacttataa tcctgttgca ggtacgtatt atgttgttca aaatatttaa taaaacagca
     1261 caaagagatt tctttgtgct gttttattat ttttagttgt tgacaaacac taaaatataa
     1321 aatataatca acacataaac gaacgtatgt tcggcaagaa agcaggggat ttttatggat
     1381 gagatggttt taagtactca gaaatggcta aacaagaaat acagtaatgt tacagggttt
     1441 gataaggttc cagaaaatgg gcgaacaggg tggcctacaa tctatggact tattgaagga
     1501 ctacaagtag aacttggtat tactaatcta gtagctaatt ttggacctac cactgaaaaa
     1561 atgtacgata atcaggttac tcctaaatgg ggcaaaaatc tacctaaaaa tattgtattt
     1621 cttattcaag gtgccttttg gtgtaagggg attaatccag gtggcttcga tggggtatat
     1681 actccatatt tagatactgc tgtaaaagag ttgcagactg atgcagggtt taatggaagc
     1741 gctgtaactg gagttttgga tgcgaaatgg gccaaagcgt tatttgatat gagtgctttt
     1801 gttcttgttc ccggcggaga tagtaaaatt agaaaaatgc agcaatggtt aaatgtaaat
     1861 tatttagaat acacgggaat aatgccttgt gatggtattt atcaaagaaa ttcaaatcaa
     1921 gcattaattt ttgcattaca agctgaacta ggatattctc ccagtgaagc aacaggggct
     1981 tatggtaatg ggacaactag taaaacttat cctgtgtctg aaggtaactc gggaaactat
     2041 gtaagaataa ttcaatatgg attatatgtt aatggttatt ttgaagacgg tacattcgat
     2101 ggtattttca ctaaatatat gggtttagaa gtactggcat tccgaaaatt tatggtattg
     2161 cctgaatata ctgaaatagc agatgttgtt gtaataaaag gactattgtc tagcgctgga
     2221 gatattagaa gatctgcaga tggcgcagat acttctacgc aattaactag atctcaaatc
     2281 caaacattgg ttgataatga tataaaaata gttggtagat atcttactgg tactgttgga
     2341 attggaaaag atgaacgaaa taagtatcta actacagagg aattgaataa tattttttct
     2401 aaaaatttat ctgtgtttcc tatctatcaa gatggaggtg cagcattagc ttattttact
     2461 tatgatcgtg gcttatcaga tggtaagaag gctatagatg cagcaaaaaa tctaaatata
     2521 ccattaacaa cagttattta ttttgctgtt gatctagaca tgttagggga agaaattctt
     2581 gcatatgctg gagaatattt taagggggta tctgctgtaa tgtcatatag cgggtatcaa
     2641 acgggtgttt acggaacaag aaatgtttgt tctcaaataa tcaataacgg gtatgcttcg
     2701 tttgcatttg tttcagatat gtcgactggt tatagtggga atttaggttt tcctatgcct
     2761 agacaatggt cttttgatca atttgttgaa tttaccatag gaagtggcaa cggagctgtt
     2821 ggtatcgatt taatcgctac atcgggtaga gatagcggat ttaatgagtt atcaaatgat
     2881 actaataatg ataattacat tgccaaatac aatcagaagg ttataaatca gatggtacgt
     2941 gcgtatcaat atctatcaca agctggtctt gataatcctt ggaaccctca tttgacattt
     3001 tatagatatg taaattattc tggtttgtca tgggacatca tttcttctcc tgtaacagag
     3061 catgatagaa aaatttatga tgaatatagg aataaattaa ctaatagcga gggcctgtat
     3121 aattatttta tagatcctaa tactggttca ataataggtt tgcctcactt aattgtgacg
     3181 ttacaatcac agatgtttct agccgacaca atcaatgata gtgtttctga ctttgcaggt
     3241 tggttaggag atttaatgac ttgctggggt gaagttaaga aattaggaat acaaatggag
     3301 caaggtgtat ttgcattagt aggtacggct gcaggtggaa catttagtct agaagattta
     3361 taccaagatg ttgacgcggt aaatgtttat accttagcaa aaagaaattc tgattcaaca
     3421 gctttatcgg ctttaaaaac gtactattgt gataagggtg ctattgatag atttaatcaa
     3481 tttgttaaga acagatttgg ttcttcagat aaaatcgctt cagtaacaaa tagtttatta
     3541 gaaaatgacg aaacagttga tatcttaggt gtagggataa tttcattttt cattgataaa
     3601 atagttaata attcacttgg gacagatttt acttatcttg attttattgg agatgagggg
     3661 aaagttgcaa atggattttc tgaaaaaatt attcaatttc aagaaaatag aagtaaactt
     3721 aattaagaat cgtttactat ttaaacagtg tccttttcct ttttaaatat atacgataaa
     3781 ataagtatat gaattgtaaa aggacatgat gattatggaa gaaatagaat ggaataatct
     3841 gactcaaaat aataaaaata attctggatg tggaattatc tttatgtttg ttatagtttt
     3901 gggaatagta gtacttttaa taactgtcaa gatagtagat tactttgcga atactcctga
     3961 agaacaaata cagagaaaac ttcctgagtt tgaaagagta ccaccttatg atccaagaga
     4021 taattcattt caagtaaaaa aattaaaaga gtctccaaat gaaatgtggt ttgaagttac
     4081 agttgaatca agcgacttca aagaaaaaaa gaaagaatac aattatattg taaaggcaaa
     4141 agtatcaaaa agatttattt ttaaccctaa attagaaata tatagtgttg taatagaata
     4201 atagtgttaa aaaaattgta aaagttaaag aagtcaaatt ctgatgtgaa aaataaaata
     4261 taccatggaa gacataaaag aaaaactgga taactagttt atttaatggc agaccgttaa
     4321 ggcctgccat ttttatgtat tttagcggaa taaatataat catgcgggag tgtaaactta
     4381 ctgtttcaaa aagtatactt tttgacatgc cttgattttc tttgtttatg tatggcataa
     4441 taaccacata atacaatttg aaacgtttct gatttaatct gattgttttg aaacaatttt
     4501 agtgaaaaaa gggtagataa aaacgatgag aaaatttttt tatatccggg tcagtagtta
     4561 tgatcaatcg atcgaacgac aactgatttc cgcgaaggaa ttaggaatcc ctgaagaata
     4621 tatatttgta gaaaaagcca gcggcaaaga ttttaaacgc cctgaatatc aattaatgaa
     4681 acggatgttt cgaaaaggag atttactcta tctacaatca ttagatagac taggcagaaa
     4741 taaacaaatg attttagaag aatggcgaga attaactaag gaaaagcaga ttgatatttc
     4801 tgtgttagac atgcctctgt tagatactgc tagttacaag caactagatg ggttagagac
     4861 gcttatctct gacttaattc tgcaattact cagctatatg gccgaagacg agcggaaacg
     4921 aattaatagc cggcaaagag aaggcgtaga agctgcgaaa caaaagggtg taaaattcgg
     4981 cagaaagaaa attgctttac ctcctgaatt tcctgcgatc tattctgact ggaaagcagg
     5041 aaagataact gcagtctatg ctatggatca attgggcatg aaacgaaata ccttttatcg
     5101 acgggtgaaa gaatatgaac ataccttgca ccacttttct tgatagatat aacagctatt
     5161 agacgggaag ggagagtttg aaaatggact taacgaatca aagggtcgtt tttactggaa
     5221 cgctgcagca aatgacacga acccaagcca tcggtttggt tcactcttta aacggccatt
     5281 gtcaatcaag tgttacttca aaaacaaatt tcctggtttg tggtcagtat tctaagtcgt
     5341 tgtttgatga ttctctctat acaaaaaagg aacaaaccgc gcgggacttg attgcgttag
     5401 gtcatgagat taccatttta tcagaagtcg aattttatgc gttaattagt caacagttga
     5461 aagaatggca acggtattta taatcgttag taaaatttac aaggaggaaa atttttatga
     5521 agcgaaaatc ttatgatcgt tggggcattg aaataactag ttttaatcga aatattgctt
     5581 ttcacgtgga gtgtgactgt ggggaattag caaaaagtgt ttatcaaaaa aattactttt
     5641 actgtcctaa atgccatcgc cgatacgggg tacatggaac atagaaacga aatagtgcgg
     5701 tagaaaagcg aatgaatgta gtatatttct acaaatcatt cgctttcttt attttataaa
     5761 tagttggtcg agagatcccg ttttcttttg caatctctgc aactggaatg ccttgcttta
     5821 acatgtccac cacggtttta aaaactaagc gtttttgcgg atctgttgag ttaatagaat
     5881 actcgattgg tcgtccctta tatcggccgg ctttttgagc caataatact ccttgccgct
     5941 gacgttcacg aattttctca cgctcgtttt gtgcaatata acttagcagt gataaaaaca
     6001 tatcgaacat agcggtatcc aggagttcat ttccagtatt aaaatctaaa aactgtgcat
     6061 ctaatatttt taacgctact tttttctgtt tcataaaagc aactgtattt tttatatctt
     6121 cataatctct tcctaaacga tctagtgatg taacgatgac gcaatcacct tcacggatat
     6181 aatttagtaa tttttgaaac tcaggacgct cagtatcttt accactggct ttttcttgga
     6241 ataatttatc aatgttaagt ccttccatca ttgccaattg tcgatccaaa ttttgatctt
     6301 gtgaagatac tcgaatatag ccaatttttc ccataaaaaa ctccttttta aaagtgatat
     6361 aatttacatg taaaatttac ttgtagtttt actgctatca tagcattttt aaaaataaat
     6421 tttatgtaga cttaaaattt acttcatgga gggtttaaat ggatgaaatc aaaagtaaca
     6481 aaaaagcatt tggcatttat ttaggagaac ctagtacaga acaattaaac caattttttg
     6541 gttctgttgc gaagtttgaa aatcaaacgc gccctctctg aactccaaat atcttaggct
     6601 gttattccca ttaatacctt gatttcagta gacaccgaaa agccgaagag cgttccattt
     6661 cttcggttct ttttatatat tcctcgaatg gtctccatgc ccttaatcgt ggaagaggct
     6721 gtacggagac tttgataaaa tttatttcgt cgtttaatag gtcgatggtc ttgttctatt
     6781 aaattgttaa gatacttcac agttcggtgc tctgtcttag tatataaacc cacactctgt
     6841 aactttctaa aggcggagcc aagagaaggt gctttatcgg tcacaattgc tttcggctca
     6901 ccaaactgtt tatggagtcg ttttaagaaa gcataggctg cttgcgtatc ccgtttcttt
     6961 cgtaaccaga tatctaaggt taagccgtcc gcatcaattg cacgataaag ataatgccaa
     7021 cgtcccttaa ttttgatata ggtttcgtcc attttccatg aatagaagga ttgtctattt
     7081 ttcttcttcc aaagataata gaggactttg ctgtactctt atacccaacg ataaatcgta
     7141 gtatgacaaa catttattcc acgatcatat aacaattcct gaacttcacg atagcttaga
     7201 ttgtaacgca ggtagtaacc aacagcgaca ataatgacgt cttttttgaa ttgtttgcct
     7261 ttaaaatgat tcattactct gtcctctctg tcttttttct caattttaca ctaaaataga
     7321 ttttttg
//
LOCUS       DACECJ010000106         6924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.109, whole genome shotgun sequence.
ACCESSION   DACECJ010000106 DACECJ010000000
VERSION     DACECJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.109"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IWO09_002553"
     CDS             94..1248
                     /locus_tag="IWO09_002553"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0265118.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IWO09_002554"
     CDS             complement(1270..2022)
                     /locus_tag="IWO09_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0265119.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IWO09_002555"
     CDS             complement(2386..5052)
                     /locus_tag="IWO09_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0265120.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGLGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IWO09_002556"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IWO09_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0265121.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2044 a         1420 c         1252 g         2208 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgagtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaa
//
LOCUS       DACECJ010000107         6770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.110, whole genome shotgun sequence.
ACCESSION   DACECJ010000107 DACECJ010000000
VERSION     DACECJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.110"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..852
                     /locus_tag="IWO09_002557"
     CDS             <1..852
                     /locus_tag="IWO09_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0265122.1"
                     /translation="LYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTP
                     LEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSL
                     IEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKL
                     NKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKEL
                     HASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELI
                     PLENELR"
     gene            complement(2787..3452)
                     /locus_tag="IWO09_002558"
     CDS             complement(2787..3452)
                     /locus_tag="IWO09_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0265123.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            3578..5614
                     /locus_tag="IWO09_002559"
     CDS             3578..5614
                     /locus_tag="IWO09_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0265124.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            5618..6256
                     /locus_tag="IWO09_002560"
     CDS             5618..6256
                     /locus_tag="IWO09_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0265125.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            6284..6712
                     /locus_tag="IWO09_002561"
     CDS             6284..6712
                     /locus_tag="IWO09_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265126.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         2281 a         1015 c         1249 g         2225 t
ORIGIN      
        1 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
       61 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
      121 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
      181 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
      241 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
      301 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
      361 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
      421 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
      481 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
      541 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
      601 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
      661 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
      721 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
      781 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
      841 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
      901 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
      961 aagacagcta ttgaataatg tttcctccac ctttttcatt catttacccc accccgttgt
     1021 tatatgaccg tgttttgaga ggaaccaata aaaagcatag gctaactagg ggttcctttt
     1081 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag
     1141 tgattttttg ctataatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag
     1201 tttattgaga aaataggaga gtatttgata ctaactagta ggaaaaaatg agataaaaat
     1261 taaatggaat catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac
     1321 ctatgggact gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg
     1381 tcgtattgat tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt
     1441 ctctatatga ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg
     1501 gaataatcca ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta
     1561 ttcgtcctct tttttgacca gattttgacc agtctaattt acattctaat atattcatat
     1621 gagctaattt tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
     1681 gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
     1741 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
     1801 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
     1861 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
     1921 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
     1981 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
     2041 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
     2101 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
     2161 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
     2221 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
     2281 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
     2341 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
     2401 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
     2461 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
     2521 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     2581 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     2641 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     2701 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     2761 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     2821 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     2881 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     2941 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     3001 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     3061 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     3121 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     3181 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     3241 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     3301 tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc
     3361 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     3421 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     3481 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     3541 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     3601 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     3661 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     3721 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     3781 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     3841 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     3901 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     3961 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     4021 agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt
     4081 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     4141 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     4201 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     4261 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     4321 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     4381 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     4441 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     4501 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     4561 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     4621 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     4681 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     4741 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     4801 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     4861 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     4921 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     4981 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     5041 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     5101 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     5161 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     5221 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     5281 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     5341 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     5401 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     5461 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     5521 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     5581 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     5641 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     5701 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     5761 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     5821 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     5881 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     5941 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     6001 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     6061 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     6121 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     6181 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     6241 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     6301 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     6361 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     6421 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     6481 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     6541 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     6601 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     6661 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     6721 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta
//
LOCUS       DACECJ010000108         6475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.111, whole genome shotgun sequence.
ACCESSION   DACECJ010000108 DACECJ010000000
VERSION     DACECJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.111"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..3097)
                     /locus_tag="IWO09_002562"
     CDS             complement(<1..3097)
                     /locus_tag="IWO09_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0265127.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RA"
     gene            complement(3111..4328)
                     /locus_tag="IWO09_002563"
     CDS             complement(3111..4328)
                     /locus_tag="IWO09_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0265128.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4318..5913)
                     /locus_tag="IWO09_002564"
     CDS             complement(4318..5913)
                     /locus_tag="IWO09_002564"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0265129.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6038..>6475
                     /locus_tag="IWO09_002565"
     CDS             6038..>6475
                     /locus_tag="IWO09_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HAQ0265130.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYL"
BASE COUNT         1829 a         1338 c         1081 g         2227 t
ORIGIN      
        1 atgctcgctt catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat
       61 atccttcgta atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg
      121 gcatatattg cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa
      181 aggcatgaat caaattatca cttgcttgtg taaaatagcg acgtttaatg acaaagatat
      241 cttcattact atctaaagtc cccacatcaa atgcatttaa aatttgtcgg taaacttctt
      301 tgactccttc tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg
      361 aactattcgc agcataagtt cctagtaatt gatcaatata agtagaatca atatcataca
      421 acttagtggt attttcacta taaaattcga tcgttgaaaa atcttctccc tctttttctg
      481 gttcttcctc catcaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa
      541 gttgaatttg attgttcaaa gttgaagatt gcaccatgtc atcattgtaa tcatcataag
      601 tcactaacga ttcatacgca cgattcaatt cttgaaatgc tttgacgtac tcgatacgga
      661 tttcaagtgg cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat
      721 atgcttgctt gaatcttttt ttggattctt ggtaacttgg ataaatcaaa cccgattctt
      781 ctttagcttc tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtcgctggct
      841 ttcggaaagt aacaattagt ccttttgttt tttcaggata cgtacggttc gtacgtgaaa
      901 aggcttggat caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag
      961 gggcgtcaaa acctgtcaat agacgatcca ctacgattac taaatcgatt tgtttcccaa
     1021 attgtttgaa ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct
     1081 caatgtctgc tagtgaccag gcagtatcat aatactgatt atattcctca ataatttttt
     1141 gcatttcttc ttgttgggca cttgaatctc ggctattttc ttctaatgaa taggtaatag
     1201 cgattcgtgg gaaatcagga tcctccatcg ttcgtccttc acggataggc tgattgggaa
     1261 actcttttat taaccaatca gattctttcg tcatctcttt gatggcccga taatactttt
     1321 tggccatatc aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa
     1381 aatcaaattt tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat
     1441 gttcctctgc ttcaaaaaca gtagggtcca aataactttc ttttttgaca gactccattt
     1501 gatcgatcat acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta
     1561 atttccgata gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt
     1621 gaaatcctaa aaccgagcca tcttctaatg cattcttgat ggtataggtg tgcaagacct
     1681 ctccatactg atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag
     1741 ggaaaatcgg tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt
     1801 cttccatatt ttcagcactt aacgctcgat gacactcatc aactacaaac acgatatgtt
     1861 gccccattaa tttttggaag cgattggttc cttttttctc ttcctgtttc tttgcatatt
     1921 tcaacgccgc atctagtttt tgacgggttg taatgatcac aacattcgca ttggcatctg
     1981 ccaacagagt ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt
     2041 tggcattacc agaagaaacc ccagtattaa actccgaagc aaatttcgta aactctgtgg
     2101 tggtctgatt atctaaatct ttgcggtcca ctaacatgat cgttcgatcg atccccgatt
     2161 ttcgtgctaa aagcttagta gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat
     2221 gccacacata ccctgattga tgtttatttg cggcaataaa caaggcttga atcgcatgca
     2281 cttgataagg atgtaacacc atgagggttt tattgtcttg atcttcactg acaatcgtat
     2341 aatccgcaat caagcgatgg gcatctggga tatttagcac ttgtttgcaa aactcatata
     2401 agttttctac ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat
     2461 ccttcggtaa ggcatttgca aaataccgag tcgtttgttc attggaaacc acaaacaact
     2521 gtaaggtcga aaagatattg ttccgaaaca tgccttcttc agcatatttt ttgatttgat
     2581 taaacgcctg ataaaaacca tcttttgcag ttacttgttt tagctcgatt tggataatcg
     2641 gtaagccatt gatcaataag gtgacgtcaa aacgtcgatc ccgcgcttct atgttacttc
     2701 cacgttttgc aatttgatgg accacttcat aagtagagat tcctccacca atgtcttgat
     2761 tggaataaag gataagtgag acagaaccca attgcggatt ttcccgttca atcgtgatcc
     2821 gagccatccc attttcacct tttaaccatt tcgcagcatc aaatggggtt ttcgtccgta
     2881 gcaataattc ggttttgatc gtttcaaact ctttatcggt caaaggagaa tcattcagtt
     2941 ctgcttgatt atttgagatg attttttgac gtaaattgct ccaaagatct tcttctgatt
     3001 ttagatccgg acgatacgtc cactgattat gtccttcccc gaggacacga atcaactgtt
     3061 cttcgacttc cgcttcctca cggtgactaa ttttcattct tcttccctcc ctaaacaaac
     3121 atcttttgca agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
     3181 gcgatcgtat catctagctg cttgaagaag gcaccaattt tttgttgttc atttatatcc
     3241 ggtggaaact gaaatgtcct ttgttctata ccacctttag tcacatgtac cattgtcgtt
     3301 ccgttagttg attctttaat tttctcttta tcagtcaata gccaagtata tagatactgt
     3361 ttgtccaatt ccttagataa aaatttaagt ttccaaatat gataatggta gattacacgt
     3421 tcttcattcc acatttcagg accgaagctt gttgcccata aatataagag atctccttta
     3481 ttagcatact tgtcttcctc taattctaag tttgaataat accatctatc attagtatta
     3541 aaattaccta ctcttaaaac cctatatttt ccagaatcaa gtaattcttg ttgtttaaaa
     3601 gctctaccat tcaaaaaaac taccacttcc cccaacttcc gctcttccca atctcccgta
     3661 aatcctggaa aacgaacttc tggcactttt gctccgtttt tagggaacat tttttgtaag
     3721 aatccttttt tcgtttcctt cagcaaatct aattcgtgct ggtgaagcat gatggtgtga
     3781 tcaagttgtt tgaaaaatga accgatcttg atctgttcgt cataagttgg aagtgagata
     3841 ggtgtctttt taccactatc aattgtatag gttccgacgg ttccaatgcc tgcaacgtca
     3901 cggatatact tctgaatttt gttatttatg aacgagtagt aaaagaagtt tccgtcaatt
     3961 ttatgctcat tcttgaacca aatgatgttt ccatccttaa agtaaattgg gttatcatca
     4021 gtgattaata aggggacacc tattgatccg acaccagtta caagcaagtc tccttgagaa
     4081 actttacctg aatttttcga atattcatta tatttttctt ctgaaatgta aagataatcg
     4141 gatggttcct cattttttgc agctgagaca atatcacgag cacgtaaaaa tctaatacct
     4201 gagttagtcc aatctgattg atgaattcgc ttcacagaag taacatccat catatctcct
     4261 aacttacgct cttcccaatc ttccgtaaaa ccaggaaacc ggatttctgg tcgtgtatca
     4321 ttgctcatgt ttgaacacct ccagcgcacc ttgtaaccac gcttcatttt caggtgttgt
     4381 ttgcagggaa gagatagctt ctaaaagttc tttttctagc acttgctgtt cttgtcgtat
     4441 cttcttgatg tcatttccga catgaaccat atcaattggt tcttcttctt cgaaagtatc
     4501 gacatagcgt gggatattta agttatagtc attttctttg atttcatcaa aggttgccaa
     4561 atgagcgtat ttctcgacat cttttctttc agcgtaggtt tcgaggattt tttgaatgtt
     4621 ttcttccgat aatttattct gatttttccc tttaacaaat tcacgactag catcaatgaa
     4681 taatacatct cgagtttgac gattcttctt caatacaatc accgtcgttg gaatcgatgt
     4741 accaaagaac aagttcgcag gcataccgat cacagcataa atactgccgt cttctaataa
     4801 tttttgacga atgacccctt ctgcagcacc acggaataaa accccgtgtg gcaagacgat
     4861 agccatagtc cctgtttcct tcaaatgata gaaaccatga agaagaaacg caaaatctgc
     4921 tttggatttt ggcgctaatt tcccgtagcg attaaaccga gaatcatcta agaaggtcgt
     4981 atctgctgac caattagccg aataaggtgg attcatcact actgcatcaa atgtgtaagg
     5041 ttcatctgtt ggccagtcct tattcaacgt atccccattg cggagattca tttcttcggc
     5101 atctacccca tgtaaaatca agttcatttt tgctagattg tatgtcgttg tatttagttc
     5161 ttgcccatga tatttaacgt tatctggatg agttaagtag ttacgtacat ttaacatcaa
     5221 ggaacctgaa cccatggttg ggtcaaagac actaaagaat cgtcgttctt tttgatccaa
     5281 cgtcacgatt tgtgccatca tatcagaaac catatgaggc gtgtaaaatt ctccggcttt
     5341 cttccctgct tctgaagcaa attgactgat cagaaattca tacgcatccc cgatcacatc
     5401 cccatcgtga cctaaaactt ccacgtcatt caatttcttg ataacttcgg taatcgtcac
     5461 atttcgttgt tgctcatctg ttcctagttt ttttgattgg agatccacat catcaaataa
     5521 cccattaaat tgattgtagg tagatgccaa ttgcacaaag gctttgttta gatcgttcaa
     5581 ttggaacgtt gcttgttttg cttgatccgc taagacgttg aaaagatact ctggtgagat
     5641 cgaatacccc aaaatatcaa cgattgtggc aatcaggtct tcttttgatt cttcatctga
     5701 aagtaattca cgatacaaca ttgtttgttt gcttactgtg tcatattcct ctaaggattc
     5761 atctgctaaa agaaccactt gttccaacaa acggtcggat aaatatttat agaaaatcag
     5821 tcccaataaa taatttttat attctgacgc atccatttta ctacggagat tatctgccgc
     5881 actaaataat ttttgattta attctgcacc caaaaagata cactcctatc tcttatttaa
     5941 agttgctata taccctctta ttttagcata ctccctttta gaaaaagcaa aaaaaaaaga
     6001 aaaaatctta ttcttgataa caagctacgt gacgtatatg cctaacaaaa atagaacttt
     6061 tgaatcaatc ttaaatgaag gtttaaccac agaaccggca tccattattt ttgatgaagt
     6121 tatcgatgac gttgaagata gtaagtatca taaaaattta aaagaacttt tttattttgc
     6181 actcaacaat ggaccaagtt tgttcactgg tatatcagta cctgatagtg ataatccatt
     6241 aattatggca aaaatttact taacaaaatg gtgtaataaa tacatcaggg ataggaataa
     6301 cccagcatta aaaaaacctc ttaaaacatt tggcgaaaaa gatgctgctt taactactag
     6361 agtagcagca aatgctaata ttgatgatca aaaagtattg aatgactact tgcgtggaca
     6421 ttttttatat atgagcgctg agaacatgaa cggaagtatt ttagaggaat attta
//
LOCUS       DACECJ010000109         6428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.112, whole genome shotgun sequence.
ACCESSION   DACECJ010000109 DACECJ010000000
VERSION     DACECJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.112"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..257)
                     /locus_tag="IWO09_002566"
     CDS             complement(12..257)
                     /locus_tag="IWO09_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265131.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(274..1125)
                     /locus_tag="IWO09_002567"
     CDS             complement(274..1125)
                     /locus_tag="IWO09_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0265132.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1291..2295
                     /locus_tag="IWO09_002568"
     CDS             1291..2295
                     /locus_tag="IWO09_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0265133.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(3078..3635)
                     /locus_tag="IWO09_002569"
     CDS             complement(3078..3635)
                     /locus_tag="IWO09_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265134.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(3814..4425)
                     /locus_tag="IWO09_002570"
     CDS             complement(3814..4425)
                     /locus_tag="IWO09_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265135.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(4538..4795)
                     /locus_tag="IWO09_002571"
     CDS             complement(4538..4795)
                     /locus_tag="IWO09_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265136.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(5392..5670)
                     /locus_tag="IWO09_002572"
     CDS             complement(5392..5670)
                     /locus_tag="IWO09_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265137.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(5704..5955)
                     /locus_tag="IWO09_002573"
     CDS             complement(5704..5955)
                     /locus_tag="IWO09_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265138.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1991 a         1234 c         1021 g         2182 t
ORIGIN      
        1 ttccattctt tctatttttt cggtatcaag gcaaaaacag gtagttcagg tgaataaatg
       61 acttcatgtt cgtgccaggg tttgaccatt agcgaatgat cgaaccgttt tttcagttct
      121 tcgatgattt gatcatgtgt ccagtttctt gcaggaaaat gttggatttt ctctgcatcg
      181 atcaaaaaga caaaatcgga atgaaaagtc tgggtaactt cttccgagag agaattcaag
      241 cgttcgattg gatccataaa atcacctttc gacttaaaat tgggtagtat ctgggtcttt
      301 tgctttgccg actacaccat ctaactgatc gatttgtttc atttcttctt ctgttaattc
      361 aaaatcaaat aattcagtat tttctttgat tctgcttggt gtcactgact taggaagagg
      421 aaggaattcg tgttgcagcg accaacgcaa agcaacttgt gcaatcgtct tgtcatgtgc
      481 ttcagcgatt tgtttcattt caggcacatc gaagatttta ccagtgccta atggactata
      541 tgcttcgagg atcatatcat gtttttttgc atactcaact acagttgtct gtaattcgcc
      601 tggtgcaagg aaaacttgat tgaccatcgg cataattttt gctgtttttg ctagtgtatc
      661 tagatggtgg ggtaagaaat tgctcacacc gattgctttg attttacctg cttcatacag
      721 ttcttcaaat gctcgccatg tatctgcatt tgcttgttcc caattatcac gaaaagcaac
      781 agggtttggc cagtggatca aaaacagatc taaataatct gtttggagct tcttcaatga
      841 ttcttcaaaa gaagacatca caagttcata gctgtggttt gcattccata attttgttgt
      901 caagaatatc tcttctctag gaattccact ttccttaatc gcttggccga cgctttcttc
      961 attcttgtat ccttgggcag tatcgatatg gcgataacct gcagccagcg cttctttaac
     1021 agaggaaaca gcgacatccc catcaggtgt ttgccatgtg ccgaatccta ctaccgggat
     1081 ttcgtaacca tttgatagac gataagtgct tgttaatgaa ttcatacttt cagctccttt
     1141 tttattcgtt caactataag tgtagcgcat ttttcgaaaa aaagaatcca ttttgtttct
     1201 actcttgaaa tttttgttta aaagaaaatg attttgattc tggtattttt gatttttttt
     1261 ccgtaaacta agacagaagg gaatgagaaa atggatatct atttaaaaaa agcaattctg
     1321 catattattg acagagaaac tggttctcca gtcttttcac aaaaagagct ggatctgaca
     1381 aaagaatata ttcgtgattt tcttcaaaag aagatccaaa aaatctcttc tgctcaaaca
     1441 aaaactggac aactagcaga agaaagcatg atcgttgaat cacttaaaat cgctcaagaa
     1501 aattttgtgg aagcaagtga agcaatcgtt cagcggtggt tcgatatcta ccaagaaagt
     1561 gaagaagcac ctagtgcaga tgtatttgtt gtcttgtatg aattagatac agtgatgtac
     1621 ttatctttat tgaaagtcaa ttatcgtgag gcttacactc actttgttga agcagacgaa
     1681 gcagggatcg acaaccgctt gattttgaat cgagcaattc taggggcgaa aacacaaaaa
     1741 gcagatgaag cattcgctgt gaatttgact gattggacct acgaattaat tgaaaaaaaa
     1801 tatgagtttt ctggcaaaaa agagctttat ttttctggaa gagtgatcga aagtgttcct
     1861 gcaccttctt tagaagaaaa tgtaaaagtg atccaaaaag tagccaagca tcttggaaaa
     1921 aaatttgaga cagaagaatt tgatatcatg gctgatttga aagaagctgt ctatgatacg
     1981 atagaagaaa aaggtcggtt ggatcatgag atgattgcgg aaaaagtctt caaggaaaac
     2041 atcacggcaa aaatggcttt taaagaagaa gtacaagagc aaggatttgt cccagaagca
     2101 cctcctgtaa aagaagtaca agaaatttct gagaaaaaat ttggaaaaca aaaattcaag
     2161 ctatcaaacg ggatcgaact gatcgtacca gtagatgtat atcgcaaccc agatttgatt
     2221 gaatttgtga ataatccgga tggtacgata tccgtgatga tcaaaaatgt ggatgaagta
     2281 ttgaatcgtt tataaaaatg actagaatat taggtgttca agtaccagtg atcagttaaa
     2341 tgaattgtgt caagagaagg atcggaaaag acgaatataa caaaaaaaga gcgtgagaca
     2401 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
     2461 actccttctt atttcttgga gttaaacgct tctgtcccag cctccttatt ccgaataaat
     2521 ggtgtccaaa agcttgcatc tgccagatta tctcatttct tatgacaagc taagcaaatt
     2581 atctccacca aataatttat tcatattttt gacttgcgca tactaactaa ttcttaaatt
     2641 ttcatacaaa atttctcaat ttcccttaac gagtaatatt tatgtacggt gattctcact
     2701 agtatcccaa tcacttttta agttatcccg atttcaattc tacttcaaaa atagttgctt
     2761 ttatgtatgc tgttagttga ttagatggtc atttgttgag ctaggtcgtt tattttaaac
     2821 aagttgtttc tttagcttat acctatctcg tctaccaaac gcaagatggc aaacacaaat
     2881 gcatacgcat ctgtatttgc cactttgatt cgtataaagc taacaactat taactcctct
     2941 cagctaatag ttgctctctt ttttattcag attcattttt gctgatcaca gcctagtatt
     3001 tcaatggttt tgaacactaa accaaattgt tccattacaa cattcactgg ctgatgaaat
     3061 ggattagtag atttcgatta ttcaaagcga ttttctattg gtggatcgtg ttcccaacta
     3121 ataagacgga ttccaaacct atcgtacaga tcttctaaac cacttataaa ttttgcacct
     3181 atttcaggtg ttttattgcg tggatattct attgttacgc cattaaatgg ataccaatta
     3241 ggattaggct ctcttttttt ctgtacatct cccatagaat agttagcact ttctgggaca
     3301 taatatattc ttcgaccatc ctctaattca tctaataaga tatttcgtga tcgtctatca
     3361 cttgctaaat gtagacgcca cgtgcgatac catgccatgc tacctgcatc tgcagaaaat
     3421 ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc tacttcatat
     3481 tctaatctaa aatcaaactc ccctcgggta atttctggtc ttggtggctt aggacccaat
     3541 attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac aacaataatg
     3601 attaatattt tgtttctatt tgataaattt ctcattattc gcacatcacc tctacattct
     3661 cacatattag ttcgtaatac tcctttaata tcaaaatatc tactggtcgt aataatattc
     3721 gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta cttgctgatg
     3781 atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt taacaatcgg
     3841 ttcagatatt tcccagttga ttaaggtaat tccgtattgt gtcaaaactt cttgcgcttc
     3901 tccgaaatta agtacagttc ttatgtatcc actttctcct aatataaagt ctacctctct
     3961 tttaaaacct tcctcgagat ttctaagggc tatatgtggt tttaatgaca ttgattgtac
     4021 attttccccc atataatatt gcgcacttcc tcgactaaaa tagattccta gatcgtcccc
     4081 aaaaggtaga tcttctctac cactagctaa tctacttctc catcttcgtt ctcttttcat
     4141 agaacctgcg ctgaactcac acccgttaaa ctcagctatg acagtatctt caattatgtt
     4201 aatctgtcca tctagttcgt attcaatcct aaatggaaat tctccatagg tgatttccgg
     4261 ttcgggtatt ttaggtgaga ttaatgcaaa gaaaggaaat attaagcttg gcaacatcaa
     4321 aaaaactcct cctatgatca caattcccga tgtaatccaa aatataattt gtacaattct
     4381 cattgatcca ttgtattcca ttttattccc cctattacta ttcatatccg cctatctata
     4441 tcgatcttcc aatgcagaga taattgcttc cataccttga ttttttactg agttacctat
     4501 gcgagatatc attctccact aaggtgcatc atattgtcta tctaacatga ttaattctcc
     4561 aataggtgta tactcctcca aaaacaacat ttaatgcatc tccctcgaca acataagcta
     4621 ccatgtttgc tgtatatgtg tttgagtcta attcataagc agataaattt actgtcgctg
     4681 gattaaatat aatcgcattc gtatttatag ctactgaatt tgctgcttca gcaccacctt
     4741 ttgaatgtcc gatcattgtg atttcaatag taggatttac gtggacagcc ctcaccgttt
     4801 accctgacca ttatagaaat cgcttacttt tttgaactac gcaacaaagg atacaatgct
     4861 cctaacttca ctgtctccat ttttgtccac taaggaatat aaaaaatagg cattaattca
     4921 tttaattcgg tcacaacacg ttacctttta aatcattata ctgtatctca tacattatta
     4981 tcaataaact attataaaaa tgcaaaaaat gctttatcaa tctattttgt aattggtcat
     5041 aatatcattg aaatcatacc ctgtatcgat ttttcctatg aatctcgtct atttgtgatt
     5101 gacgaaaaag aggcaagaca gacactggaa tggtaatttt acccactatt ttgctccaac
     5161 tatttgtaat cgtacccact tttgaaatac cgagatgatc attttaattg atatgctgat
     5221 tcccaagcaa aaccccaaga aattaaatgc tctatcgcat ctagcttctt gggttttgct
     5281 tatataaatt ccaacattta aattttttat gctgttcggt ctcagaaatg ccgtttttgc
     5341 ttatatcaca atatcaagat gtcaaattat tcgtgttgcc gatgactttg attaattttt
     5401 ttcaaattgt ttattaagac taatgctgtc tttttgcaga taccaatgtt cgaacttttt
     5461 aacattatca tatgatcctg caagttttaa cttcttacca ttagatgtta tcaaagttat
     5521 aattaacctt gcctttttcc cattaggatc ttttatttta tgaattttct gcaggtaatc
     5581 tctcctaatg gcagctattt ttacctcatc agtgctgata aaccattgat catccacata
     5641 catactgcct gttatgttgt gtttttccat ttcttccgag aacgaaaagt ttaaggctcg
     5701 ttcttacttc caaagttgaa tgagtccatg gattggactt agtgtgagaa ttgagaacaa
     5761 caataagaat ccatatcctc ccgcaaacat ccctaaggct atactgtctc tccatccata
     5821 agtaggatta gaagagaaaa gagtcaccgt ttgtaataat atgaaaagga ataatccagc
     5881 acaaacacca aagataacaa ctccaaacat tgaattttta tacaatgacc atttatttat
     5941 aggcttttct ttcattttac ttccccctac tgttctgttc acacggaatg aattcaaagt
     6001 tcataagccc tcctctttga acatatgaat acgacttgct aactatactt taaaaaagct
     6061 tgttttaagc ggttataggc gagttgtaat ctctattcat ctcattagca aataacttgc
     6121 cgaaaagtct actgaaatta aagcagatag tggaagaatt atttagccag gatagacact
     6181 atttgtgaaa cacatgtctt tacttttgaa attctatgat gaaaagcctt ggaatacagt
     6241 agctcttaat tggtaatact ttttccatta taaataacct cttttcattt tatatttatc
     6301 ccctctaaaa ataatatcta atctaatatt ttaaacttat atttccctcg ctgtgctaat
     6361 agagcattac taaataaata gaaactagaa aaaagcatta cgcctgccag aataaaccaa
     6421 ttatttgt
//
LOCUS       DACECJ010000110         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.113, whole genome shotgun sequence.
ACCESSION   DACECJ010000110 DACECJ010000000
VERSION     DACECJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.113"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWO09_002574"
     CDS             334..714
                     /locus_tag="IWO09_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0265139.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWO09_002575"
     CDS             696..1610
                     /locus_tag="IWO09_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0265140.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IWO09_002576"
     CDS             1855..2544
                     /locus_tag="IWO09_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265141.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWO09_002577"
     CDS             complement(2665..2751)
                     /locus_tag="IWO09_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0265142.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWO09_002578"
     CDS             3427..4377
                     /locus_tag="IWO09_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0265143.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWO09_002579"
     CDS             4398..4931
                     /locus_tag="IWO09_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0265144.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWO09_002580"
     CDS             5567..6100
                     /locus_tag="IWO09_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265145.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACECJ010000111         6285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.114, whole genome shotgun sequence.
ACCESSION   DACECJ010000111 DACECJ010000000
VERSION     DACECJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.114"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWO09_002581"
     CDS             <1..177
                     /locus_tag="IWO09_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265146.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWO09_002582"
     CDS             190..690
                     /locus_tag="IWO09_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0265147.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IWO09_002583"
     CDS             787..2634
                     /locus_tag="IWO09_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ0265148.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IWO09_002584"
     CDS             2637..3533
                     /locus_tag="IWO09_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0265149.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IWO09_002585"
     CDS             3583..3972
                     /locus_tag="IWO09_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0265150.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6285
                     /locus_tag="IWO09_002586"
     CDS             3959..>6285
                     /locus_tag="IWO09_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0265151.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLE"
BASE COUNT         2118 a          932 c         1347 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaa
//
LOCUS       DACECJ010000112         6140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.115, whole genome shotgun sequence.
ACCESSION   DACECJ010000112 DACECJ010000000
VERSION     DACECJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.115"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..324
                     /locus_tag="IWO09_002587"
     CDS             <1..324
                     /locus_tag="IWO09_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0265152.1"
                     /translation="FIIPPVNEKWSDYTGLSQEMLQGFAKKIKFQLNSQGFNRIADFV
                     NQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEENHITASKYHLDDAFFSKSWQHQIPD
                     KLQLK"
     gene            465..1547
                     /locus_tag="IWO09_002588"
     CDS             465..1547
                     /locus_tag="IWO09_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0265153.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1547..2350
                     /locus_tag="IWO09_002589"
     CDS             1547..2350
                     /locus_tag="IWO09_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0265154.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2420..3160
                     /locus_tag="IWO09_002590"
     CDS             2420..3160
                     /locus_tag="IWO09_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0265155.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            3171..4175
                     /locus_tag="IWO09_002591"
     CDS             3171..4175
                     /locus_tag="IWO09_002591"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0265156.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(4220..6064)
                     /locus_tag="IWO09_002592"
     CDS             complement(4220..6064)
                     /locus_tag="IWO09_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265157.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         2005 a         1010 c         1227 g         1898 t
ORIGIN      
        1 ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc tcaagaaatg
       61 ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt caatcgaata
      121 gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat ccatctaggc
      181 tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga aaatcatatt
      241 actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca acatcaaata
      301 ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt gttttcttta
      361 tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa aaatcataag
      421 caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat acaaaagaag
      481 aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa gaagaggtaa
      541 gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat gacggtttag
      601 gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt tctggacata
      661 tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg gaattccaaa
      721 ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc aaaacacgtg
      781 aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta acacctcaag
      841 taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca tcaagccaag
      901 aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat attgaataca
      961 aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt atcgggacag
     1021 ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat gttttattcg
     1081 caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt acacaccttg
     1141 taaatcccga tattgctttt gctttagata ctggtacagc aggagacact ccaggaatga
     1201 caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt tttgatgcat
     1261 ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag gaaatggata
     1321 ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa atgcatttga
     1381 caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg cattcccata
     1441 catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca gaattagtga
     1501 aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga ataatagaat
     1561 acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat ggggaatcaa
     1621 tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact tttttgacgg
     1681 tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc tagtcgataa
     1741 tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag ttgtacgggg
     1801 tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt ttggttcgat
     1861 acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt taatctttct
     1921 gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca gttcaaatgc
     1981 ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt tatcgatgat
     2041 agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat tccaatttgc
     2101 tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga aatatggctg
     2161 gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt ttatcggctg
     2221 gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg tttatgtgaa
     2281 tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc cagacgccta
     2341 ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg taggataaat
     2401 ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag cgtatcctta
     2461 taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt tgttgatcga
     2521 tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg atctagatgc
     2581 tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat tacagtatta
     2641 ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg gacacacaaa
     2701 agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac atccttattt
     2761 tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa caatccatcc
     2821 agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat tcgtatttac
     2881 aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg atttgtttct
     2941 agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca cttcaaaaga
     3001 agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac acctacctca
     3061 gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca aagggattga
     3121 tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac atgatttttg
     3181 taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat ttgctgcaag
     3241 aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca atcatcatcg
     3301 gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa aatggcattc
     3361 atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga aacctgaact
     3421 ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa gtaacacagc
     3481 caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa ggccgaaatg
     3541 atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc aacggtcgga
     3601 tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc aatgcattaa
     3661 aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct cgtgtaacga
     3721 atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa tttcgtcaag
     3781 aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg tacgaattga
     3841 ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga aattgggatt
     3901 ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt tctatcggtt
     3961 cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa atctttggcg
     4021 atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga cagaaatatg
     4081 ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat tttggtgcga
     4141 tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg gaatccacat
     4201 atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt tgaacagtga
     4261 tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga tccgctacgt
     4321 ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg atgcctcgac
     4381 taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa tgttcctctt
     4441 gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca tgagtttctc
     4501 taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg ttgataggaa
     4561 gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca gctaacttct
     4621 ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac agcatttcga
     4681 aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt tggtccgtat
     4741 gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt gtatttactt
     4801 cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt ttgattttta
     4861 agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt agattgtagc
     4921 tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc gttgtttgat
     4981 agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc aagacgatcc
     5041 aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg catgcaagtg
     5101 aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat tcaggaagtt
     5161 ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt tggaacgtac
     5221 aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag cagatgatga
     5281 aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat ggttcaaaaa
     5341 gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa gcaaagcaac
     5401 ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag atcgtataga
     5461 aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc ggcactgatt
     5521 gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc ggtggcagtc
     5581 cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg atataatttg
     5641 ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga taatctttcc
     5701 gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt cctttgatta
     5761 gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat gtttcgtctt
     5821 tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca ggatcatctt
     5881 ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc gcaggagaaa
     5941 aaataaaata aaagaaataa gcaattcctg ttaaaaataa caatgataat ataatgatta
     6001 tgctccactt tatacgtaaa taaaaagatt cttttttatt atatatgtta ttaaaaaaag
     6061 acataaaggt attcctttct aaaacttgta atgttagggt aaacgttttt taaactaacg
     6121 tcaattattt ataataaaaa
//
LOCUS       DACECJ010000113         6133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.116, whole genome shotgun sequence.
ACCESSION   DACECJ010000113 DACECJ010000000
VERSION     DACECJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.116"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IWO09_002593"
     CDS             complement(207..1169)
                     /locus_tag="IWO09_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0265158.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IWO09_002594"
     CDS             complement(1171..2610)
                     /locus_tag="IWO09_002594"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0265159.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IWO09_002595"
     CDS             2795..4741
                     /locus_tag="IWO09_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0265160.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IWO09_002596"
     CDS             5005..5877
                     /locus_tag="IWO09_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0265161.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1954 a         1056 c         1080 g         2043 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aat
//
LOCUS       DACECJ010000114         6114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.117, whole genome shotgun sequence.
ACCESSION   DACECJ010000114 DACECJ010000000
VERSION     DACECJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.117"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..798
                     /locus_tag="IWO09_002597"
     CDS             <1..798
                     /locus_tag="IWO09_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0265162.1"
                     /translation="GDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISH
                     YFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIAN
                     DWQVPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSV
                     NDSIEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKG
                     YKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            809..3013
                     /gene="topB"
                     /locus_tag="IWO09_002598"
     CDS             809..3013
                     /gene="topB"
                     /locus_tag="IWO09_002598"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0265163.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3259..3852
                     /locus_tag="IWO09_002599"
     CDS             3259..3852
                     /locus_tag="IWO09_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0265164.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            3868..4767
                     /locus_tag="IWO09_002600"
     CDS             3868..4767
                     /locus_tag="IWO09_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0265165.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            4770..5255
                     /gene="ssb"
                     /locus_tag="IWO09_002601"
     CDS             4770..5255
                     /gene="ssb"
                     /locus_tag="IWO09_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0265166.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5621..5881
                     /locus_tag="IWO09_002602"
     CDS             5621..5881
                     /locus_tag="IWO09_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265167.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2223 a         1064 c         1251 g         1576 t
ORIGIN      
        1 ggtgatccta aacagttaaa attttttgag agtagtattg atttacttag ctacgctgca
       61 ctgaatcgtg atcagttaaa cgatacgtgg ttagtctcta tggaagggtt aaaacaccat
      121 gtgatcagtc attattttgg tgaggcagta tctgaattga gaaagaaaca agcctttcct
      181 caatccatcg agatttgtgt ggataatgac cgtgcaggac atattttcta cgaaaaagaa
      241 caattaatgg gcgcagttga tccatttacg aatcagaaag tacgttgtga acgaggaatc
      301 gctaatgatt ggcaagtgcc aagagaatac aaagtcattt atgaagaagt cgcaaaagaa
      361 gaaaaggtca caccagaagc tattatggcc atacacaaga cagaaaacaa tctgcaatta
      421 acgaaccaac tggtttccgc tcacaaagtc aatgcgtctt ttggtcagca gctttctgtt
      481 aacgattcaa tagaagtcat tagtttgaaa gatatttgta gagaagtcgc aaaagaatta
      541 aaagattgtg aacgctcgaa tggtacgtat gattttgatc gtttttatca aggaaaaggg
      601 gatattaatt ctcagattct tttctcttat aaagcagaac aatactacaa aggctataaa
      661 aatcatgaac atgaatttgt acctgaagtg aaaaaggatt ggaacgatca attgaagcat
      721 gagatccatc aacaggaaat tagaaagcaa aaacgtgcga tgttgtttca acaaggcaga
      781 caacaagaaa gggagtgaga tcgtcacgat gaaaactgtc attttagcag aaaaaccgtc
      841 acaagcgaag gcctatgccg atagcttttc taaagccacc cgaaaagacg ggtattttga
      901 aatccaagat cgattgtttt cgggtgaaac tgtgatcacg tatggttttg gtcatttagt
      961 cgagttagat tcgcccgata tgtacgatga aaattggaaa caatggtcat tagagcattt
     1021 gcctattttc cctacacatt atcactatca tgttcctaaa gataagaaaa agcaattcaa
     1081 cgtggtgaaa cagcaacttc aatcggccga caccattatt attgcgacag atagcgatcg
     1141 tgaaggggaa ctgatcgctt ggacgatcat tcaacaagca ggcgctgatc aaggaaaaac
     1201 ctttaaaaga ctttggatca actccttaga aaaagaagcc atttatcagg gattccagca
     1261 attacgagac gcagaagaga cctatcccaa atttgaagaa gcacaagcac gccagatcgc
     1321 tgattggttg atcggcatga acggcagccc actgtatagt ttactcttgc agcaaaaggg
     1381 cataccagga agtttttcct taggcagagt ccaaacccca acgctttaca tgatttatca
     1441 gctgcaggaa aagattagaa acttccaaaa agagccttat tttgaaggga aagcccaagt
     1501 aaccgctcaa aatggcatat ttgatgcgaa gcttgatccc aatgaaaccc aagcgacgca
     1561 agaagcattt gaagcctatt taaaagaaaa aggtgttcaa gtaggaaagc agccaggcac
     1621 gattcttgaa gtcgaaaccg agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc
     1681 cagccttcag tctaaaatga atcagctcat gaaagctagt gccaaagata ctttggaagc
     1741 catgcaaggg ttgtatgaag ggaaatatct aagctatcca cgaacagaca ccccttatat
     1801 tacagaagga gagcatgcgt atctgttgga tcatttagat gaatacaagc attttttaaa
     1861 ggctgaatca ttccctactc ctattcatac accgaatagt cggtatgtga ataataagaa
     1921 agtccaggaa cattacgcga tcattcctac aaaaacagtt atgacagaag caacgtatga
     1981 aaagctgaca cctttacaac aagcgattta tgatcaagtt ttaaagacga cggtggccat
     2041 gtttgcggaa aaatatacct atgaagaaac tacgattttg acacaagtcc agcagcttca
     2101 gctaaaggca gtcggaaaag taccagtaga tctaggttgg aaaaagctat ttggcaaaga
     2161 gagcgacaga aaagagaagg aagaagaacc gctacttcct aaagtaacaa aaggagaaac
     2221 ggtgacggta gatctacaaa tctttgaaaa agaaaccaag ccaccacaac cttacacgga
     2281 aggtacacta atcaccgcca tgaagacagc tggtaaaacc gtggatagtg aagaagcaca
     2341 atccatattg aaagaagtgg agggaattgg tacagaagcg acacgagcaa atattattga
     2401 aaccttgaag caaaaggaat atatcaaggt agagaaaaat aaacttgtgg tgacgaataa
     2461 aggaattttg ctttgtcagg ctgttgaaaa agaaccgctg ttaacaagtg ccgaaatgac
     2521 cgccaaatgg gaaagctatc ttacaaagat tggtgagcaa aaagggacac aagctacttt
     2581 tctagcaaat atccaaaaat ttgtttccca cttgttggaa gtcgtcccag gacaaatcca
     2641 agccactgat tttggagcaa cgttacaaga ggtgaaagta gccagtgaaa agcaagaagc
     2701 ggcgcgtcat ttaggatctt gtccaaagtg ccaggaaaaa gaagtcatac tttatcagaa
     2761 ggtggcgact tgcacgtctg aagcctgtga ttttaaattg tgggtaacga ttgccaaaaa
     2821 gaaactcaca gcgacacaac tgaaagagat cattcagaac ggccgaacaa gtcaaccagt
     2881 cagaggacta aaaggtcaga aaggttcgtt tgaagcaacg atcgttttaa aagaagactt
     2941 tacaacagga tttgagtttt ctgaaaagaa gaagaccacc tacaaaagaa aagtgagacg
     3001 taaaacgagt tagaattgac ggagggaaaa acgatgagaa atacaacgca taaagaacaa
     3061 gaaatcgaaa atcaaaaagc caaaatacgt tcagagcagg agaaaatgcg tgccagcaaa
     3121 cgaagactac agcaattgaa gaatcaacaa cggattcaaa aagaacttga attagatcga
     3181 taaatcctcc tgttgttaat attaaactaa catgttatag ttatgttagt ttaatattaa
     3241 cattcgagag ggaaagcgat gaaagataaa gtcaagtgca tttttgagaa atacaatggt
     3301 aatctatcct tgaaggatgc tagaaatgaa gggttatcac ctatgacgtt agatcgttta
     3361 gccgaaaagg acaaaataga aaaaatagca tcaggttttt ataccttaaa agatagtttt
     3421 gtggatgaat tatatctcgc tcaaagtatc tatactagag gaatcttctc tcacgagaca
     3481 gcgcttgatc tttttcaagt aggaacatat atccctaaaa aaattaatat gatgtttcct
     3541 aaaggataca atgtttcgaa agagaattta gatcagtatg ccattcaacc gcattacacg
     3601 aataaagaaa actttgaact gggaatcaca gaggcagaaa gcttttacgg caataaaatt
     3661 ttcgtctatg atttagagcg aacgttatgt gatatgtgga atcccagata taaagcaagc
     3721 gtagaagtaa aacaagaagc attaaaagaa tatatgacaa gtaccagcag aaatacaaat
     3781 aagctaagaa gatacatgaa tattttaaaa tcaccaaaag aaatgacttt gtatatgttg
     3841 ccactttatt gagaggagta aaggtgaatg acaccgacac aatttaaagc gaaaacaaga
     3901 aatgttgcag ctgagaaaca tttaaaccca caaattgttc aaagatatta tatgatggaa
     3961 aaattattag aatgtattgt tgaatcgcct tatcatgatg attttgtatt aaaaggggga
     4021 tttctgatcg gatcaaaata cgggttagaa aaccgttcaa ccgttgatat tgatacgact
     4081 tatcgaaatt cgaaactaac aaaagaaaaa ttagaaactg ttttttctga attaacagct
     4141 gctcctacaa aagatggcat tcggtttgaa ttaaagggct taacagagac gagggaagcc
     4201 gattattacc caggttttca agcaaagttt attgcttttc ttgaaaatgc acgtgtacct
     4261 tttaaattag atattacttc aggtgattct attattccag cagcattgat atatgaacat
     4321 aaattgatgt ttgaagataa aacaattcat attccagctt atcctacgga acaaatttta
     4381 gctgaaaaat tacatgccac gtttagtttc ggaatagata attcacgtat gaaagatttc
     4441 tatgatctgt atatgatccc taaactagaa cgtatcgatg agataaattt gtataagagt
     4501 gttcaaggaa catttaagga aagaaataat caatcagtgt ttaagaattt gtataatcag
     4561 gaaatgcctc aattgatgaa gaatgaggga ttgagggaga aatggaaaag atatcagcaa
     4621 gaaaactatt ttgccaaaga tatttctttc gaatctacct taacttctgt tgattcgctg
     4681 atgcaatcaa ttgtcgagca agaggaagta gaacgaaaac aaaattttaa gagatcacaa
     4741 caaatgaatc aagaaatgga gagataaaga tgatcaataa tgtaacttta gtaggtcgat
     4801 taaccaaaga tcccgattta cgttacaccg caagtggcac tgcagtgacc acttttacct
     4861 tagccgttaa ccgtaatttt actaatcaaa gtggcaatcg agaagctgac tttatcaatt
     4921 gtgtgatttg gcgcaaacca gctgaaacga tagcgacatt agctaaaaaa ggcagcttga
     4981 tcggcgtcgt cggacggatt caaacgcgga cttatgacaa ccagcaagga cagcgtgtct
     5041 atgtgactga agttgtggcc gataatttcc aactattaga aagtaaagca gcgacagaaa
     5101 gccgtatgca cgctgatcaa tcgtccagca gtccaagccc taccaccttt gaacaacggg
     5161 atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt ggtggacaat
     5221 caatcgatat tagcgatgat gatctaccgt tctaagtagc attttctatt ggttgcattt
     5281 gctacggttt atgttataat ttagacaaca aaaagaattt tcaatctata accaaaaacc
     5341 actagctggc gggctagtgg ctttctttta cgtttctatt ataagaatta acgggtaggc
     5401 ttactgtctt tcttggcaaa tttacaatca agccatttgc agatatagtg cgcggctact
     5461 tctgccatga ttgcaaaaac aaaatcaaaa agaattttca aacctataac ctcctcccct
     5521 tgccagatta ggccggggta gcagacagca agactattat agcataattg aaatatccaa
     5581 atgattggat ttttgctata attttagcga ggtgattgtg atgaacaaat atgttggttt
     5641 gcttgataaa atccgagtaa taaaaacgca accgttattg gttcgcttta ctttacaaac
     5701 gattcatgaa tcgattaatt gtgtagttgc agatattgaa attatcgata aattgctaat
     5761 tatggatgat ggaaaataca atattgccgt cacaggtcac ttcaataaac gaaatcagct
     5821 agttattgct agcatgtatg ttagaaatcc cgatcatttt acgcgatcga tgggcatata
     5881 aataaaagaa gagttgtagt cctaaatgag gattgcagct ctttttgttt tacatagaaa
     5941 caggaaaaga cctttttagg aagacacaag tgccttctca attgaaaaac ctttctctca
     6001 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctgcacgtt cactaagata
     6061 tggtgtcttg caaagcaaga caaaatagac aaaacttcct ctcaccaaaa ggtt
//
LOCUS       DACECJ010000115         6030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.118, whole genome shotgun sequence.
ACCESSION   DACECJ010000115 DACECJ010000000
VERSION     DACECJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.118"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..95
                     /locus_tag="IWO09_002603"
     CDS             <1..95
                     /locus_tag="IWO09_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0265168.1"
                     /translation="NHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(181..1275)
                     /locus_tag="IWO09_002604"
     CDS             complement(181..1275)
                     /locus_tag="IWO09_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0265169.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1464..2225
                     /locus_tag="IWO09_002605"
     CDS             1464..2225
                     /locus_tag="IWO09_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0265170.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2340..2780)
                     /gene="fabZ"
                     /locus_tag="IWO09_002606"
     CDS             complement(2340..2780)
                     /gene="fabZ"
                     /locus_tag="IWO09_002606"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0265171.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3000..3755
                     /gene="fabI"
                     /locus_tag="IWO09_002607"
     CDS             3000..3755
                     /gene="fabI"
                     /locus_tag="IWO09_002607"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0265172.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3865..4878)
                     /locus_tag="IWO09_002608"
     CDS             complement(3865..4878)
                     /locus_tag="IWO09_002608"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0265173.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5043..5963
                     /locus_tag="IWO09_002609"
     CDS             5043..5963
                     /locus_tag="IWO09_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0265174.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1849 a         1197 c         1075 g         1909 t
ORIGIN      
        1 gtaatcatat tccaagaaaa tcattgaatt acagaacacc aatcgaaata tttttgagct
       61 atgtacaaga agcattttat tctagcttaa tttgacaaat cataacctat aaaaaaacaa
      121 aataaaaaag cagaaataac tggctatttc tagcggatta tttctgcaat aactaattat
      181 ctatttttta ttttcaatta atttaaagct tgaccatggt ttcaaaaagt caaggaggaa
      241 taattcgtca tcggagattc ttcctacaac attgacacgt ccgtcgtttt tcatatcctt
      301 caaggcgatt tgtgtttctc ctttgtattg accgtaccct tcattgtcaa tcagtacatc
      361 cccatgatgc atatctcgcg tattatgagg cgggaaatct ttatctttgt agtatacacg
      421 agtcatcgtt gaacgaagga tatattctga gcgatcccct cgataactat gaaggttatc
      481 aaacaagcag attcgttcgt tttctgtaat accttcttcc gtatctactt tcaatgttgg
      541 ataatccgcg ttaaatgctt ctgccatttc tttcaattct tcttcacttg cgtacgcatt
      601 tccaatagaa atatcatcaa tcaatcctgt aaggattaaa tgcttcactt gtgtcgcaat
      661 ttcaagatct cggtggtctt ccagcgtgca caagccatct tgcgtaggcc aagggccaaa
      721 ctcagcagat tgcgaattga cgaatgccat cgtattcaaa ttatattttc tgaatttttc
      781 tgaacaaaaa acaaagtgtt cgtatcctaa accagagtat cgatgaggat aaaaattatg
      841 agaacctaat aaattatccg tgtttggcga ataactcatg atgttgtcaa cgtaattcgt
      901 gcccgaactc atattgatct cgattttgat accaaaagga ttgcgcgtca tttttgcttc
      961 ttctgctcct gtgaatccga tatctaagcg gaccccgtag gcacccatct tatggaaaaa
     1021 agataagtca tcgtaagaaa tctccaactg ttcaaataac gctggattga tatcaaccat
     1081 tacttccatg cctaggctat tcgcgtaatc aactacttct ttaaattctg aaagaacttt
     1141 ttctttatca tcattgatct gcagcaaact ggtaaaaacc cgtttatacc catacttatg
     1201 agctaaatcc aagtacgctt tgtccttttc aaatgttgac cgttctggat aaatcgagat
     1261 tcctaatttt cccatattac catttcctcc tgcatctttt cgtttttgta tccacttaca
     1321 ttatagcaaa cacaaagtaa atggtctaca ccttttcttt ttcttttaaa ataaatgata
     1381 aattccaata atttaagtag gtattttccc tctattttgt tatcattaaa gataacaaaa
     1441 tagtaaattg gaaaggaaca gttatgaaga aaaataaagt tattttaagt attagtttag
     1501 cagcagcatt gctgtttgct gctggttgtt ctgcaaacaa cacgacttct tctagttcgt
     1561 ctacagaaag ttcaaaaagt acatctactg cggaaagcac gactgccagt agcgtcgatt
     1621 tgaacgcatt ggaattacca caattgacga aagatgtcgc cgaaaatgaa gatctggtcg
     1681 aaatggtgac gaataaaggc acaatcaaaa tcaaattatt tcccgaacac gcaccaaaag
     1741 cggttgaaaa ctttatgaca catgcaaaag acggttacta caacggactg actttccatc
     1801 gcgtcatcaa agatttcatg atccaaggtg gcgatcctaa aggcgatggc actggagggg
     1861 aaagcatttg gggagaagga tttgaaacag agatcaacaa ccacctttat aatataagag
     1921 gtgcgttatc tatggctcgc tcacaagatc ctaacagtaa cggcagccag ttctttatcg
     1981 ttcaaaatac agatgatatg catgatggtc ttttaaaaga cgactatcct caagcaatca
     2041 tcgacgctta taaaaacggc ggttatcctt cactagatgg ccattatact gtcttcggcc
     2101 aagtgattga aggaatggat gtcgtggatg ctattgcaaa tatcgaaaca gatagttcag
     2161 acaaaccaaa agaagaagta aaaatcgata aaatcaatat actgcaagaa gcaaaacaaa
     2221 aataaaaaaa taacgtctga atcctgcata gatgacaacg tatgctagga ttcaggcgtc
     2281 ttctttttac ataaaaagca agtaagcttc cttttgtttt gctatatcag gttttatagt
     2341 caagcatttt ggttgacgat gaacgtgaac tcgcactcac agactttttt atcatctaca
     2401 tatgctgcag ataacgctgt accgactgat cccttcattt tggtgatttc aaaatgacat
     2461 ttcagcacat ctcctggaac aactttttga cggaatttgg ccttattgat cccaccgata
     2521 taagctcttt ttcctagaaa ctcttctgat tttaggataa gtatcgaacc tgcttgtgcc
     2581 agtgtttcta gaatcaacgc acccggcatc actggattgt caggaaagtg accttggaaa
     2641 aaatcttcat tgattgttac attttttgtc gcaacgatct tttttcctgg ttctaaagta
     2701 tctacataat caatataaca aataggataa cgattaggaa tcaattccat tacttcagta
     2761 gcagataata cgtaagacat aattaaactc cttctttcag gcaattgcca ttttcattac
     2821 tttgactatc aaagtatcat attcctaaat atttagcaat ctttttttaa tttaagaaaa
     2881 aatagtttga caatcaaaac attttgacta ataattaaga aatcaagaag ttattttagc
     2941 tattattctc tattttttta cgactatcaa atttttatca gaaacaaagg agagtcatta
     3001 tgtcatttct agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa aaaagcattg
     3061 cttggggttg tgctgaagca atgttgaaac aaggggcaga aattatctat acttaccaaa
     3121 atgaacgaat gaaaaaatca ttagtcaaat tggtcggcga agaagcattc actgtagaat
     3181 gtgatgtttc tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt gaatatgctg
     3241 gggagatcca cggccttgtc catgcagttg cttacgccaa caaagaagaa ttgtccggaa
     3301 atgtgtctga tatttccaga gacggctatc aattagctca agatatcagc agctactctt
     3361 taattgctgt atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg atcgttagca
     3421 tgacttatct aggttcagaa cgcgcaatcc cgaattataa catgatgggt attgcgaaag
     3481 cttctcttga agctgctatc cgttatttgg cagctgagtt ctcgcctaaa ggaattcggg
     3541 tcaatggaat ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt aaagactatc
     3601 aaaaactgat ccagttatct gaatctcgta cgcctgatca gcaaggtgtg acgatcgaag
     3661 aagtcggtaa tgcttgtgct ttcttaatca gcccactttc cagcggaatc atcggtgata
     3721 ttatctacgt tgacaaaggt gtccatcttt cataaacttt tgttgcattc tttaaagtaa
     3781 aacatataaa aatcaggaaa aaacttttta aagtctcttc ctgatttttt ctttttacgt
     3841 ttttttcaac tcattacttt gaaattattc cacgacttca atccagcctt ctggtgcttc
     3901 tatgtctcca aattgaatac ctgtcaattc atcatacaac cgttttgtta ctggtcctac
     3961 ttccgtttca ctataaaata catgaagatt gttattgtga taaatcccac cgattggaga
     4021 aataacagcc gctgtaccac atgcaccagc ttctgcaaat tgatctagat catcaatgaa
     4081 aatatctcct tcaattgctt gcagtcctag tcgttcttca gctaactgca acaaggaata
     4141 tttcgtgata cttggtaaga tagagggcga ttgcggagtg atgaattttc cgtcttttgt
     4201 aattccaaag aaatttgctg cacctacttc ttcgattttc gtatgtgtgg ctggatcaag
     4261 ataaacacaa tctgagtaat ccagttcttt tgcttctgtt cctggaagta aactcgctgc
     4321 gtaatttccg ccgacttttg ctgcaccagt ccccttagga gcagcacgat cgtaatcaga
     4381 tacaataaaa ttcgttggtt ttagtccacc tttgaaatac gctcctactg gcgtacagaa
     4441 gacgacaaat aaatattctg gagctggatg gacaccaatg ttttcaccga ccccaatcag
     4501 taaagggcgc aagtataatg tagcgcctgt tccatagggc ggcacaaaat cttcatttgc
     4561 ttttaccact tgcttgacag catcaacgaa catcttttca gggaccattg gcatcagcaa
     4621 tcgtctagca ctttgattca tccgtcgact attttgttca ggtcgaaaga gattgacctt
     4681 gccgtctttt cgacgataag cttttagacc ttcaaaacat tgctgaccat aatgaagcgc
     4741 tggagagcct tcatgaatcg tcaggtaatt atcttctgtc aattctcctt cttcccattg
     4801 tccgtctttc catttggcaa taaaacgcca aggagttttg atataagaaa aacctagatt
     4861 gttccaatcg atttgcatat acttaccacc ttcgttattt tctccacatt ttatcattat
     4921 ccatattatt tgtcatttta ttttcagctt ttttccttca ttatagttat ttttgctatt
     4981 tccttttttg aaaacttctt ggtacaatac agaaagaatc aaattttaga aaagaggatt
     5041 ttatgttttc attattagca actgccggta cgacagactc ttctggcgag acattgacag
     5101 atcaagctgt cagcaaagta aatgcgtttc agcggttttg ggacgggatt gattgggacg
     5161 ccattttggc tgcctttatc caaaaaagtt tatatctgat ttttttgatc gtcctttttt
     5221 ggattcttca ccgtgtggga aaatatctga ttgataagtc ttttaatcag tattctaaaa
     5281 aagccattct aaacgagagc cgcttaaaga ctttacatag tcttttaaat actatttttc
     5341 attataccct tggtttcttt tttatctatg caattttatc tgctatcggt gtgccgatcg
     5401 gatcactttt ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt gcccaagggt
     5461 tcatgagtga tgttattacc ggatttttta ttatcatgga acaacagatg gatgttgggg
     5521 actacatcaa gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt ggtatccgta
     5581 cccttcagct gaaagcaacg gatggtactg tccatttcat cccgaatcga aacatcacca
     5641 cgatcagcaa tctctctcgt gcaaatatgc aagtactttt agatattcgg attgttcctg
     5701 aagaaggata tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca ttagctgaaa
     5761 aataccaaga agagctccaa actgaacctt ctatctttgg attagtggat cttggaaaca
     5821 gcaattttgc gattcgtacg gtctgctacg cattgaacgg aaaacaatat gccttaaaag
     5881 aagagttctt gagctcctat gtcaaagaat tgacagcttc tggtttcacg attccgaaca
     5941 gtccaattgc tgtcggaaaa taatttggac aaacgatgca acgaatctca ctaaaaagag
     6001 cgtgggacaa aagtaaagaa cacttttgtc
//
LOCUS       DACECJ010000116         5903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.119, whole genome shotgun sequence.
ACCESSION   DACECJ010000116 DACECJ010000000
VERSION     DACECJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.119"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..985)
                     /locus_tag="IWO09_002610"
     CDS             complement(<1..985)
                     /locus_tag="IWO09_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAQ0265175.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="IWO09_002611"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="IWO09_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0265176.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="IWO09_002612"
     CDS             1810..2964
                     /locus_tag="IWO09_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0265177.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="IWO09_002613"
     CDS             3179..4147
                     /locus_tag="IWO09_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0265178.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="IWO09_002614"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="IWO09_002614"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0265179.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="IWO09_002615"
     CDS             5177..5785
                     /locus_tag="IWO09_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0265180.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1637 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtattg aagatgtaag gca
//
LOCUS       DACECJ010000117         5853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.120, whole genome shotgun sequence.
ACCESSION   DACECJ010000117 DACECJ010000000
VERSION     DACECJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.120"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            636..1595
                     /locus_tag="IWO09_002616"
     CDS             636..1595
                     /locus_tag="IWO09_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0265181.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1629..2054
                     /locus_tag="IWO09_002617"
     CDS             1629..2054
                     /locus_tag="IWO09_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265182.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            3206..4183
                     /locus_tag="IWO09_002618"
     CDS             3206..4183
                     /locus_tag="IWO09_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0265183.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            4193..4690
                     /locus_tag="IWO09_002619"
     CDS             4193..4690
                     /locus_tag="IWO09_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0265184.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            5066..5347
                     /locus_tag="IWO09_002620"
     CDS             5066..5347
                     /locus_tag="IWO09_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265185.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            5349..5765
                     /locus_tag="IWO09_002621"
     CDS             5349..5765
                     /locus_tag="IWO09_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265186.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         2128 a          997 c          981 g         1747 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttcgccgatt gtaaaattaa gctagacaac taaaaaagtc atttgtgcta cactcaaaat
      601 agttccgacc aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaacgg
      661 atgaacttgt aataatagag tcttatttca aaatgaatca atctgttgct aaaactgccc
      721 attgcttgaa tcgttcaaga caaacgatcc ataaagtata tctgttcttc aagcaaggaa
      781 aatcagcctt agaatattat caacagtata agaaaaacaa atcaaactgt ggtagacgtc
      841 cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt caaggatgga
      901 cacccgatgt gattgttggt cgtgcagcgt ttcctattag ttgttctgct cgtaccattt
      961 atcgtatgtt caaaaagggg ctatttgatt cttctgactt accgatgaaa ggcaagcgta
     1021 aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct attcatgaac
     1081 gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt gacactatcg
     1141 taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca aaagttatca
     1201 tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta aatcaatggt
     1261 ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga aaggaatttt
     1321 caaattggaa acagatcagt aatgtcaatg atattgccat ttatttcgct gatccaggaa
     1381 cgccgtctca aagaggccta aacgagaatt ctaacggatt gttacgtaga gatggtttat
     1441 tgaaatctat ggatttcaat tcagtagatg aattttttat tcaatctgtc gcatctaaac
     1501 gaaataatat tcctagaaaa tcactggatt atcgaacacc tttggaagta tttttgagtt
     1561 acgtaagtat tgatgatctg tctaacttaa tttgacaatt aagaattttt tgtttagagg
     1621 tgatttttat gatttgcaag aacaagaaaa aaattattgc tattattgct agtttctctt
     1681 tgatttttag tactgcatgt actgtcttat taacaaataa tgctaatgtt gaagctgcaa
     1741 gctcttctta caaacataat catgctggtt atagatgtta caaaatgagt tattatgtaa
     1801 gtccttctaa atgcaaacaa ttggctcgcc aaactaataa acttaatacc gtctcagcta
     1861 ttgcaaactt tattggacta tctggattaa caccggggat tatatcatta gttttcggat
     1921 cagcagttag tgctaataat gtatttgtta cagccgctag aagaggaaaa ggcgttcaat
     1981 taaattatat tgcccatttt agtaatacca ctacctataa ttatagttct aaccaaaatt
     2041 atgttataaa ataattaaaa ctttaaacta ggaggaatgc tttatgaaat taacaaaaat
     2101 tgctgtaatt ttagctgtta ttgctggcat tattttatat tttgtattgc ctgctaatca
     2161 attcttcctt tactttggaa tacctgcgat tttaatagta aatggcgtaa gaatctttga
     2221 acaacgaaaa aaatgtagct ctacctaatc gttacaaaac aacaatttca ccttcggtga
     2281 aataagcgaa actttacaaa aagtggctag caaatgctag ccttttttgt attgtagagc
     2341 gtgaattttg agggggattc aagggggagc ttgcgcccct ttgacaagcg gttatttgca
     2401 ccaaatacct ccgtatttgg ctagcaatcc ttctaaaaaa tacacctatc gatcagagtc
     2461 aaaacaaatc aattttcgag tgagcgaatc cgaatttgca aagctgaagc tttgcaaatg
     2521 agtgtgccgg cttccgtaaa aatacatatg gctataattt gcgttttaag gcgttttaaa
     2581 cgttctggta tataattata ccaaaacaac tttaaaacgc cttagatcta attttagagg
     2641 tcttatagaa cattttaggg ttgttagggc ttgccctgac cgcctgtaag gcgctttgag
     2701 aacaagataa gggttttata ctaagactgt cttaaaacgg cttaaacaaa cgctcagaac
     2761 ttttcaaagg agttctttat gaactgtttt taagcaaagg agcctttagg cgactgcgca
     2821 gtataatgtg agagcgtagc gatcacacat ttttagtgac cgtagggagc gagtttccca
     2881 gtttagaaaa attcgcgaac ttgtttgagg aaaaaagcag gagtttttta ggcgggaaat
     2941 tttgcctcct cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct
     3001 gtatgcttac tctcccaagg tgtgcagaat cttttggggg tctttttgtc tgtttttagg
     3061 ggtctttttg tctgttttta ggggtctttt tgtctgtttt tgggggtctt tttgtctgtt
     3121 tttaggggtc tttaattagg ggcttgaccc ctattagaaa gctctttata attttgttaa
     3181 gtagttaatc ttgaaggagg attttatggc aaatgaaatt gtgaaatacc accatgaatt
     3241 aaatacgatt cctttacgca aatttacgtc agtagaaatg aatttattct tctctatagt
     3301 atctagaatg cgtgacgtag gcgataaaaa agtacaattt acattcgaac aattaaaaga
     3361 tttaagcaat tataaagcaa ctgcaaatgt tcgattcata gatgatttag aaacaacgta
     3421 tgacaaatta atggatttac gttttggtag aagaagtgct gacggtttgc aacgtgaacg
     3481 atttgtttta tttaatcaat ttaaaatcga tggaaaagct gatattcctt ttgctgaaat
     3541 acaagtccat gagaaagcct tacccttact taataatctt gaagaatggg ttcgatattc
     3601 tctccaacaa ttcaatgaat tagaaagcag ttattccaaa accatgttta gattattaaa
     3661 acaatacaga accaaaggaa ttgcctattt ttcaaaagaa gactttcatg aattacttga
     3721 tatccctaaa agttatagag aaagtgatat aaataaaaaa attataaagc ctattcgcca
     3781 agaactaacc gcaatcttca aaggtctgac aatcaaaaag aaatacggaa aaggaagagg
     3841 aaaacctgtc attggctatc aattcacgtt taaacctgaa ataaaaaaag cagacgactt
     3901 taataaacac gcaccaacaa aagaagaaca aaataaagct ttagaactag ttaatcaaca
     3961 aatttactct gaaaaagacc ccacttcatt acaagaaaaa atcaaagaac agaaacgaaa
     4021 attagaactt ttacaacaat tagaagagct agaaagaaaa caaaataatc tgaataaaga
     4081 aaataaggag attaacgaac aaataccaga taacaattcc gatattcccc cagaattaaa
     4141 aaaaggctta ctagataatc ttcaaaactt atttaaagaa taaggggttt ttatgacaga
     4201 taaaactatc aaagaattag cagatattct aggtgtttca aaagataaag tgaagtatca
     4261 actgaggaaa ttacctagta attttaccta caaaaaagga aatattatct acctgaaaaa
     4321 agaagcagta acaacaatta caaaggtttt atcaggtaat gagccgggta acttacctag
     4381 tgaattacct gattcattac ctaccgatta cctactcaag gagattgaaa ctaagaataa
     4441 tcatattgaa gaaaaagata ggcaaattga aaaacttcaa ctagctttag aaacacaaca
     4501 aaaattactc gaccagcaac aacaactcac cctgcaagcc aaccaacaaa tcgagaaact
     4561 gcaggaacaa ctacagttga cttatacgga agaatcttcg gaaaataaat caacgactct
     4621 ttctgaagaa acagaatcga tagaaaaaca atcaaagacg gagaaaaaat ggtggcaatt
     4681 ctggcaataa aaccagcaat ttaaattaat aaaaattgct ggttttattc ttctttttgc
     4741 ttttgcgagc agagcaacat gggcccatgt tgcgatattt tggtggcgcc ccaatccccg
     4801 ttttttcgtt gaatcttact cggactgcac tcgttttaca gctgattttt ctgaattttg
     4861 aaagagcata aggaaacgtg gtatcacttt cttacgaaag gtgctagata agctgtgcct
     4921 atcacgtttc tcagaaaaaa atttgctatc acttttttgc cattgtggct aggctatgta
     4981 tagtgttttg gcgaaaaata gctatacaaa tactatattt gtatgatata cataaattat
     5041 atactgatac gaaaggaaaa tttccatgga cattttagtt cgaaatatca atcccaaaat
     5101 tattacaaga atagataaac gttgtcagga actaacaacc aaaacgggca aaaaatggag
     5161 tcgaaatcaa tacctaaaaa ttttaatcga aaacgatttt gaccacgaac ttatgaatta
     5221 caaaaaagat caattcgatt tattattcga aaaatttatt gccattcaag cagaaaatac
     5281 gaacatacta aatgactatg ttcacacaaa caaccaactc attgaaacac ttatcggagg
     5341 aatataacat gagaaaaact tctgttcaaa aacacgtaac cctttatccc agtcacgttg
     5401 acgttttaga ttccatcatc gaaaaaaatc cagacgtcaa caattattca gaagccattc
     5461 gctttttatg tatgagttat gaacctgcta ccaatacaac cacaaccagt aaccaagcaa
     5521 aactcaatgc catgggaaaa gaaatttcta tcatgacaga aatcatggca gaatttgctg
     5581 atttatatct aaaagatact ggtgttctta tcggtacaaa ttctaaagta tatcaagaag
     5641 tgaaagaaaa aattgaaaat caactcaatc cttcccgacg aaatcaaccc aaaaatcgtc
     5701 aaaaagaaac caatcaaacc tttacttctg gaaattttga cccaacaaac tttctaaaaa
     5761 gataaaaaaa gaaaccgaaa aataaaaaaa ttcctcgatt tctttttagg gatcggtctt
     5821 ccagcaagat ttgcaagttg gtaccaactt gcg
//
LOCUS       DACECJ010000118         5794 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.121, whole genome shotgun sequence.
ACCESSION   DACECJ010000118 DACECJ010000000
VERSION     DACECJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.121"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            95..760
                     /locus_tag="IWO09_002622"
     CDS             95..760
                     /locus_tag="IWO09_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0265187.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(1044..2129)
                     /gene="xerS"
                     /locus_tag="IWO09_002623"
     CDS             complement(1044..2129)
                     /gene="xerS"
                     /locus_tag="IWO09_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0265188.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2463..2696
                     /locus_tag="IWO09_002624"
     CDS             2463..2696
                     /locus_tag="IWO09_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ0265189.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2693..3100
                     /locus_tag="IWO09_002625"
     CDS             2693..3100
                     /locus_tag="IWO09_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0265190.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4237..4755)
                     /locus_tag="IWO09_002626"
     CDS             complement(4237..4755)
                     /locus_tag="IWO09_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0265191.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1950 a          930 c          915 g         1999 t
ORIGIN      
        1 aattattcct accttattat caagggaaag tagaagaaag aacatataaa aatcggttat
       61 ctacaatcaa aaaaactttt cttatttctt taaaatggag ttaagagaaa tacgtccaat
      121 tgacattcaa aagtggcaaa ataatttatt gagcaaatat aaaaattctt atgtaagaaa
      181 catgtatggg atttttcaga tgtcattaga tcgagcagtt gttttgggac tacttgaaaa
      241 aaatcctgca aaagttattg gaaatgtgaa gaaaaggaaa actacagttg atttttggac
      301 aaaagaagaa tttacaaaag ttatttcaac tttttatatt gatgatttgt accaacattt
      361 tggttttatt tgtatgtggt tactttttat gaccgttatg cgtttgggag aagccactgc
      421 attaacttgg aaagatattg attttgaaaa taaaaacttg cgtgtgaata aaacactcta
      481 ttataagaga gccaaaacct atgagtttgt agaacccgaa acggcttcta gtattcgtac
      541 aatcgaacta gacgacaata caattacctt tttaaaagct tggaaggagc gtcaagtttc
      601 aataggaggc atgtcgtttg tactttctta taatggaaca cctactcaga aatatacaat
      661 ccgtcatatt atgagaagac atgcaacgat cgttggtgtt catataatcc gcatacacgg
      721 attaaggcat agtcatgctt ctttgcttat atcacaataa aaagatgcac ttcgaattaa
      781 agaaaggtta aaacatgctg atgtacaaac gactttagga acttatggtc atttgtatct
      841 aaattcattg agagagataa caaatgatcc cacagattat atccaaacta atttttctaa
      901 aatcaaaccg acacctattt acgataataa aaaataaatg ataaacaaaa gtaggatcac
      961 accaaaagaa acacactaga aaaactataa tacaggcttt tctagcgtgt tgtttcttat
     1021 ttccgcctat ttatacatta tactcataaa gcatttaatg catcgatcgt agccgcattg
     1081 tctatatggg tatataaagt tgttgtattg gtacttgaat gtcccaattg ttgggcagtt
     1141 aataccaatg aatttgtctg ttggtagagt ctagtagcta atgtatgacg cagtttgtga
     1201 gggctgactt gaattctaaa gccttttgaa tattttgcta ccatccgttc gattgcaata
     1261 ccggatatac gcttggcttc tccttggtaa attgctagaa ataaagcttt ttcgttttct
     1321 gttactttat atcgttcttt ccgaatagct agataattag ctaaatattc cattgcgaat
     1381 gctgctatat tgacagcatc tcttttattt ccttttctag ttactaccac attattctta
     1441 tctaaagata ggtcatctaa attaatattt ataacctcgg agagtctcaa tccagatcct
     1501 aaaatcagtg cattgatcgc caggtctctt tctacatctc gttgataata gatcaaagct
     1561 cgtttactca aagtattttt atattcattg gcaatataat ctagataatt ttgtgtatcc
     1621 ttatctagaa ataatttttt ctcaatggca ttggcacgtg cagctaacgt agttttatct
     1681 gtgatggttg ctacttgatc cataggatta ttgtctaagt atggttttcc tagaggatta
     1741 ttggaacttt tcgaaagata tttaaataga acctttaatg ccgtgatatt tctattgata
     1801 gtagaagtag atagacctaa gttattatta tctttgagct tagtttttgt tgtctctttt
     1861 tttacatttt cttttgtgcg acgtttcaaa tgagatttgt acaagtttag gtcataggca
     1921 gttaactgtc ttatcgaggt gatagaagta tcaggaacga tagactcact cagaagccat
     1981 tcataaaaga gcttataatt ttttagatat tctaaagtgg ttcgaggtga ttgatcttta
     2041 tcctcacgat cttgaacaaa atcttttata taatcgggca tatgaagtat gattgtatct
     2101 attttttgat atatttctgt atttttcatg tcattcatcc tttcaataag taggggagaa
     2161 gagtatctct ataattggac tataccaatt atagaatgtt ttgtcaattg gtatgctcta
     2221 aaatttatta atttgtttca aaaaaagcat ataaacaggt atacgtttaa taatttaaaa
     2281 taaatggtat acataaatag ggaaggaaat agaagggagc aatctaaatt ttttgaattt
     2341 tcttaattaa ttatatagaa agaacaatct ttctatagag ttagacatga gagggactat
     2401 gcttgattta aaatcaacaa gcgtatatac tgtatataca aagaaaaagg agggagaaga
     2461 ctatggcaat taaagaaaga aaatttaggg aagtggggaa ctcagtagta ttaactattt
     2521 caaaggagtt tcttaaagaa aatgggttaa atcccggaga tacggttttt ttagatgaag
     2581 aaaaacttcg agcggcaatt acaaaaaaac aagccttatc tgatgaaatt gatttagcaa
     2641 tatctcaagc attaactgaa tatgatacta cttttagaga gcttgtcgat aaatgagtat
     2701 tcattaccta gaagccaaag atattataaa aatgaatgta atccaaataa aaaaatactc
     2761 tcctaatgaa ccccttggtg taaaggatgc gaatgcttta gaaatgtgtg tagggcaact
     2821 tcgagcctca gcttttggtg aagaggttta tccttcgatt tatgaaaaag cagctatcct
     2881 acttatacaa ttgataaaga aacatccttt tcacaatgca aataaacgta ctgctttctt
     2941 agctactttt gtctttttga aaatcaatgg acagctttta gctattgatc aaaaagatgc
     3001 tgtaaattta gcggtatata ttgctacgta tgataaggat ttcgatcaac taaaatatga
     3061 agtgatcaac actataaaga cacatacgca gcccatttaa ggagttactg tatgtgtctt
     3121 tatagggtta ttagctgcta tagcgaaata attgaagtaa attacaaata gctataattc
     3181 aaatccaact atatagaaaa cgtaaatttt ctatatagtt ggattaagat aacgactatt
     3241 tattttatca tatttttact ataccctata cttttacttg gtatatttat gaataatcgc
     3301 aaatttaccc ctatactact agtttgtaag ttagattaat aaaaatacta aagttatttg
     3361 ttcttttatt aaatacacct acaacttata aattgatggt atttagataa cacgaaagaa
     3421 taaaaataac agctgatagt aaagtttgtt gacgattgct actcaatcga aagtgagtta
     3481 gtgcgtatga acttgcttct gaaaacttat gaaatgaaag accttttaca tcttgcagaa
     3541 atcggtactg ggattaaact aagcaataaa aactaatgtt cccctcattc tttgaacttc
     3601 tactatttga tttatttatt ctgataaatg acgatttcac cttcaggatc ttcaaatccc
     3661 gtatagaatt tatttaagat ctcttcagta tcagtaatag gataggcgat aggccacata
     3721 ccctttgatg gttagaataa actgctcaaa atctgtatcg tatgttggct ttagcatatt
     3781 aacaaaggct atctccaaat tataaaccat aagcgttgaa tcgttcttaa caatttcaat
     3841 tcttcctaac ccaaagtcag aagatataat taccggactt gccttaccct tcatgtgggt
     3901 tgttgagatt ccatatttaa agggaattgt cggtactatc ggcggtctga aaaacatccc
     3961 aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc ctttgtagcg gatatggcac
     4021 cataatctca ttttatcgaa gtgagtcatg gtttatctgt tggccaacca aaattgatac
     4081 aatctaacga tgcaccctct acggcattct aggggagtgc acttgcattc acccaataaa
     4141 aaccggcagt tagcaaagaa cttatgtagt cgtatgttct tactgttaac atacaagagg
     4201 tctcctttcc acaattgctt agcagtaaac gggaatttac aaagctaatc tcttccattt
     4261 tcttatcttt gttctaaaag tcccaaatgg agcaactgta tttacatgaa taaatttata
     4321 tacttcccat acagccgttt tggttgcctc gtctgcccat tttcttttgt gtgcagtgaa
     4381 tagatcttca tcactcattg aatcaatcat catgttaata tcatcaatat tacgattgag
     4441 aatttcctct aattctttca atggtagatg tgcataggta tttgtaaacc acttatataa
     4501 ctctcctaac tgattccatt taaattgatc agaaggcgtt ttaacagata atccatttct
     4561 ttcatcattt tcccatttta gaattaaatt cgtccaacca acttgatatg ctaaattttc
     4621 agcaggtgtt ctatccactt cctcaactct tttatccttc aattcttcag gaatagtttc
     4681 aaattctaaa atatatttag tataagtcct atttatttct ttttttaatt cttctttatt
     4741 tttatatgtt ctcattatat tacctccata aattcctaat tgtctccttt aattcatcta
     4801 taatttcatc cacgatattt taatccgttc tacaatgcat atctgatatg cattgactgt
     4861 ctaactcata tgtgaatgat ttataaaata tcccaacgtc tcatctaaag aaatgttgat
     4921 cgtcagcaag gagctaaaaa atacgaatat atttcttttg tagtaaaatc ggactggttt
     4981 gcatatcatt ttcttcgtaa atatcaaaat catgttaact actatcaatt actaataaaa
     5041 actaccactt taagcgttaa gaactgttaa gtttagttaa gtgaggatag ttcacaaaat
     5101 ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc tttatatagg attacagcat
     5161 cgctctttct tcgattgcgc gttgggtaaa aaatattatg aagtaaaaat tgacgatgac
     5221 acgttcctta cggctcatca gatgaaacag ctacaaaaac gaaatgatca actcgaagaa
     5281 aatttaattc taaaaaagcg attgccatat tcacgcctcg ctcgacgaac tattaaaggt
     5341 caagatttac ctgaagatca tcctttttag gaaattgaag ctttttatgt tgcacaatgc
     5401 atttataata ctacaatgat gttttcacca gatattcttc tagggtgcgg tgtaatgaaa
     5461 cagatacact tgcaaaagaa aattcaccgc gaatttacta aaattatgaa taattatgtt
     5521 aatcttccga aatttttttc caaaattaag ggatcaagct ggtgttatag attgtttaag
     5581 gttggcaaaa aaataacttc tttttaaaca attatagaaa accgatactc tgttaagttg
     5641 gttgacacgg tgtcgatttt tatgactttc ttattctgaa acttcactaa cggacagtac
     5701 ctaaaaagtc tatagtatgg acgtttacga gtactatttt atcaaaaatg ctgctcattt
     5761 caacttatat gttttagtga actaccccca ctta
//
LOCUS       DACECJ010000119         5790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.122, whole genome shotgun sequence.
ACCESSION   DACECJ010000119 DACECJ010000000
VERSION     DACECJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.122"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWO09_002627"
     CDS             complement(99..1229)
                     /locus_tag="IWO09_002627"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0265192.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IWO09_002628"
     CDS             complement(1877..3079)
                     /locus_tag="IWO09_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0265193.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IWO09_002629"
     CDS             complement(3139..3342)
                     /locus_tag="IWO09_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0265194.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IWO09_002630"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IWO09_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IWO09_002631"
     CDS             4879..5691
                     /locus_tag="IWO09_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0265195.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DACECJ010000120         5666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.123, whole genome shotgun sequence.
ACCESSION   DACECJ010000120 DACECJ010000000
VERSION     DACECJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.123"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(72..542)
                     /locus_tag="IWO09_002632"
     CDS             complement(72..542)
                     /locus_tag="IWO09_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0265196.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IWO09_002633"
     CDS             872..2512
                     /locus_tag="IWO09_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0265197.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IWO09_002634"
     CDS             2666..3988
                     /locus_tag="IWO09_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0265198.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="IWO09_002635"
     CDS             complement(4056..4490)
                     /locus_tag="IWO09_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0265199.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5392)
                     /locus_tag="IWO09_002636"
                     /pseudo
     CDS             complement(4555..5392)
                     /locus_tag="IWO09_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         1696 a         1129 c         1038 g         1803 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     4981 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5041 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5101 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5161 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5221 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5281 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5341 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5401 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5461 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5521 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5581 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5641 aagaaagagg tcgtgagcct gccgtt
//
LOCUS       DACECJ010000121         5249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.124, whole genome shotgun sequence.
ACCESSION   DACECJ010000121 DACECJ010000000
VERSION     DACECJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.124"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1665)
                     /locus_tag="IWO09_002637"
     CDS             complement(<1..1665)
                     /locus_tag="IWO09_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0265200.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQN"
     gene            complement(1707..2765)
                     /locus_tag="IWO09_002638"
     CDS             complement(1707..2765)
                     /locus_tag="IWO09_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ0265201.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(2884..3339)
                     /locus_tag="IWO09_002639"
     CDS             complement(2884..3339)
                     /locus_tag="IWO09_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265202.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(3923..4726)
                     /locus_tag="IWO09_002640"
     CDS             complement(3923..4726)
                     /locus_tag="IWO09_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0265203.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1550 a         1026 c          727 g         1946 t
ORIGIN      
        1 attttgacca tatactcttt ctttcctagg tggaagacgt aaatctatat ttaaatgttt
       61 tcgtactaat tcagcactct tcaacatttc attataagca atttccagaa gagtttgttc
      121 ggataaagcc caaaatggaa ctattcgtcc aaatcggtta ataacttcag gctttagacc
      181 ttcaccaatc aaggcttgtt tgatccctga tgcgactttt cggtattctc tttttagttc
      241 agtgctgtat tcattagaat tttccttgtt tgtaacacga gatagtgagc tatatcggtt
      301 taacgaatcg aacatctccg cacctgcatt tgttgtcgca aataaaattg tatttgacaa
      361 tgaaacggtg tcgtgaactt ctcgatcatc ccatcctctt tcagtaaaac ttatcctacc
      421 ttcatctaaa attgataaaa aggcatcgag tgtttctaca ccagctttct catattcatc
      481 acacacaata atcgcagaag gatcatgcct tacatgagta cctataagtc gtctaaaacg
      541 cattgcatca tctttggttt tgtagtccgg catattaact aagagaactt tattttcatc
      601 gccataaagt cctcttgcca cggctttagc cattgcagtt ttaccgaccc ctgttgatcc
      661 tgtaaacaga gcagttgctt ttggttgctt tcgaccttta aaacgatcaa atccaagctt
      721 catacgttta aacaacattg ttatagtata aactgctaac ggttgtcctt ttatggactt
      781 taattcctct tgaagttttt ccatatcaaa atcagaatct agctgaatat tatacttacc
      841 aaatatttgc aatagacttt ctcgattata agcatttccg tctactcgat cttttgcaat
      901 caaatcctcc attacagcaa ttgatttagc cggttcggac aaatcctcac gataaagttt
      961 gtttgcttgt acgatttctt gaagtaattc atcaggaatc ctttgcgata atctaaaaga
     1021 agcctgagga tcatctttaa cagaggatgt atttagccat tgtctcaaaa tatcgacgac
     1081 aacatcgctt tttagttcat tcaattctaa cggttgtagc cgtctcccta aggcatcatc
     1141 tcttttgata tagttttcat attcttttct tgtagtagcg gtaatcattt gaagtgattc
     1201 atttgctaac ggttctttta gcgaatcacc cccaatttta ttgccactcc caaaagagct
     1261 aacaacttta tgaaactcgt caatgaagag gacaaattca ttatctttgt cctcagcttt
     1321 taaaattcgc tcatattctt tcaatcttgg aattaatgtt tctaaacgtg tttggaggac
     1381 atcatctccc ccaacacgca attgtccgat tgataattta tatacttgta ctttctttcc
     1441 atcttgtcgc tgtctttcag cccattcact tgcaagtgct gttttacctg ttccggcttc
     1501 acctaacaag atagcttgcg gtgtttccgg tctattcaag atcagtgaca actcatggag
     1561 ttcgtcatca cgtcccacta acttatgttt tggtggtttc attggtgtga gagcatgatt
     1621 aaattcggtt tgaatattct taaaaaactc ggtactattt tccatctgtt tgtgttcact
     1681 tcctctaaaa taagaataat ttacttctag tcagtcaaag ttaggtactt aaatgtcgtc
     1741 ttcatgtctc caaaagaatc tttgttaaag tcagcatttt tgtattgatc aatcaatttt
     1801 tgtgtatcat tgattgcttt aatttcggtc tccattcgac tgtctagttg acgaatactt
     1861 tcttgcgaat ctttatcgtc aggattttgt tgtaaacgtt ccagatactc agcttttgtg
     1921 gcttcaattt tcttgatgtt agtatttgaa gcatttaact gatcttctag cttatttaca
     1981 acattattct tacttgtaac tgctacaact tttgaataat caatatcgcc atctttggta
     2041 agaaagcttc cttgatagac attagttgaa tctgaatatg gatttattct gaaattgata
     2101 ttatcaggag tattaacagg ttttgctttt ttcccaaaag aaattactcc ttcattatta
     2161 acatcattca tagaatattg agaaagtgct tttgtaactg aatctgcttc attcacattg
     2221 actttggtat catcgtcagc tgtatcatca ccagaaatta gtgatacttg gtttgcaata
     2281 aaaacttgat aagctctttc ctctaattta ccttttagga ataagaaagc atccccatca
     2341 gtacttaaca tcccatagct catttcaagc ttcttaggac gatctttttc ttttgttgcc
     2401 atatataagt tatagttttt tccttgcgta gataacaact ttctagcgtt ttgggagtaa
     2461 ccaagtttta ctactgttac atctctattt tgatctgtcc acacatctga aatagtgatt
     2521 tctgctcctg atcgagcaaa ctccaatgaa gaccctatag gtgttgtatt gttcacgaga
     2581 taataattat agtttcctaa ttgataaatg tagctaccca agcctgttaa taaaaaaagc
     2641 actcctagaa aagttgcaag cataatgcgt aatccgtgac gtgttctctt atccttatca
     2701 ccaagcaacc ctccaataat actgtattga tctttggtag atgttgaatt gacgtttttt
     2761 tccatcagtt tgtctgcccc ctttcataaa agagcaaaca atcagacgtt ttttcggtaa
     2821 ttctaggttc gggattttga atcatttcgg tatattcttc cccattaata tttaacacaa
     2881 aaattatctt cctttcttcg ttcctttctt ttgctcttta ttttccaaat aatcaatgat
     2941 ttttgctatt gtaccaagat agtaattccc ctcagctttg ttttttcgga ttttgctaac
     3001 cattggagta ggtacaatct cttcatctct caattcactg aaagattttt tacctagata
     3061 ttttccaata acagatttta aaaaacgttc aaaatctttc tcgtaagttg ttttcttgcc
     3121 ttttagttca tggttcatat tgtcaacgac ttttgcggtt tcaataattg ttttagttct
     3181 cagcttaata atttcatctg gaattttcaa tcctcgtaat ttcatcgttg tactaactgg
     3241 taacccatgc tttactgcat cagcaatact aaaacttttt agatcatcta gtaagctctc
     3301 catatagtta acaaattcat gccaattaat agcattcatt gtctcacccc ttcatttttt
     3361 taaaatgagg ttcgggcgta acacatttat gttattataa tatcataata acataaataa
     3421 atcaatcagc aaatttagat atcccttaaa gtatcagcaa ttattcaaca agatatttat
     3481 tttagctatt tcagtcatca taaaagacta attagcaata aaataactaa tattatccat
     3541 ttatcagacg aaaagaaaaa acttatctaa ttactctata ttaactttta acaaatagtt
     3601 gacttgtcca aaaatgttac cttcaatctc acaaagatat taagatgttt cctaccaaaa
     3661 ctgatatcac cggacttttt attctgtgtt gtccaaaagt gttaccttta gtcgatcaac
     3721 aaattaaaaa aaattgtttt tttcttgact tgtccaaaag tgttacccat agttgttaaa
     3781 atttgtatag attagccatt ctttcgaata ttatttagac tgaaacttgc ttacaaaaaa
     3841 acttcttgca tttattgtcc aaaagtgtta cccatacttt tttaaagaag caggttgtat
     3901 taaaattaca acctgcttct tctcaatctt cttctaacca attatgcatt gaaatggttg
     3961 gcaagtttga ttctggctca ctaaaagtga atctgagccg atcaactctt cttcctttct
     4021 ttgcatagat tttttcataa tcaaaagttt caaatacctg tattccgttt tcatcaggag
     4081 ctaaaaattc ttctttcgcc tgctctagta ttcttttgtc aatatttgtc attctataac
     4141 ttgaaggtat acttaatgct cttctaaaat cttcaacttt gactacccaa taaccatttc
     4201 gccactcttt atttctgtat tgcatgagat gacgatacaa ttcttttgtg tagctacttc
     4261 ttagttttgt aaaattatca agttcaaaac gagtaaagtt tttgccaatt gagtttaaga
     4321 taaattcaaa ttcctcattt gccttaattg taactgtttc cttctgttta tctatccgga
     4381 atttttgaaa aagccacagc tgttcataga cgtctgaatc ttcaaaacgg taggttaaag
     4441 tacctagttt gtcactcaaa gcttttaaca cagaataaaa actgtccttt tctcttctgt
     4501 cgtatttgac caaataacga aagaggttga aatcaaaagt tacttctgct gttccttgac
     4561 gtttcatttt tgataaaata gcccaaaaca catccatttc aacaggctta aatcctcgca
     4621 aagctgttgt attcagacca ttgttataac gcacaattgc atttgtttgt tctgatacta
     4681 aatctacatc actatcaata atcttttcgg taacttcttg agacattgat tactctccaa
     4741 tcttcttttt gcgctccttt aatcatgtta atctggtact aattaaagga aacaattttt
     4801 tctgcttgtt acctaaatta aatcacaact aaaggtaaca gtcaacaaag atttgttact
     4861 aatctaattt tctatttttt cgccttccaa atcaagaaaa ccgaaggtaa cgtcttatcc
     4921 tagtgtgata cctcaaatgt ccacttttgt tacctttgct gtccaaaagt gttaccctta
     4981 ctgtccactt ttgttacctt tactgtccaa aagtgttacc tttatacccc tttaatactt
     5041 actctcccaa gagattaagc ctcccctaac agatatatta acagatattt aacagatata
     5101 ttaacagata tttaataaag gaggattact cctctcttat ttcaaaatct taaaaataaa
     5161 aaatcttaaa aaaaactaac actatctagg agtttatttt gcttgtgtaa ctttgaataa
     5221 ttgaaaaaaa ggaaaaggag gaagcttca
//
LOCUS       DACECJ010000122         5247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.125, whole genome shotgun sequence.
ACCESSION   DACECJ010000122 DACECJ010000000
VERSION     DACECJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.125"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="IWO09_002641"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="IWO09_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAQ0265204.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="IWO09_002642"
     CDS             1402..2802
                     /locus_tag="IWO09_002642"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAQ0265205.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="IWO09_002643"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="IWO09_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4800..5247
                     /locus_tag="IWO09_002644"
                     /pseudo
     CDS             4800..5247
                     /locus_tag="IWO09_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1675 a          850 c         1001 g         1721 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta atatattgaa tcaaaaatat aaattataaa
     4501 ataattagat ggggtatttg gtttaagtat cccttatata gtaataaatt tataggtgtg
     4561 attctcgaat taatacccga aaatgtgaga gaatttttat ggatttttta ggatacaaat
     4621 taattatttt tgcttttatc ctgattacca gtccagtact gtcggtatcc cattttgcag
     4681 caaaatcatg ttgataaagt gacaagaaga ctgatgagat atattatcca aattgagaag
     4741 ataattaatc ggattcttga gtatacccta ctttttaaga aagtagggta tacaagcgta
     4801 tgaataaaga cataactttc cctgtgatgg acgttcggct tgttccaatt accaaaatac
     4861 aggcgaacga ttataatccc aatcgagtag cgtctccgga aatgaactta ttggagcttt
     4921 ctatcattga agatggcttt actcaaccat tagtttgtta ttacaaggaa aaagaagaga
     4981 agtatatctt ggttgatgtg cttcataggt atcgtgttgc taaggaaaga ctcaacttaa
     5041 aagttgtacc ggtaacagtt attgataaac ccatataaga acgtatggct tcaacaattc
     5101 gacacaatcg tgcaagaggg acccatgaaa taagaggaat ggaaattgtc atccaaagat
     5161 tagtaaaaaa tggatggtca gacaagagaa tatccaaaga attaggcatg gacattgaag
     5221 aaatttttcg ctttaagcaa gtgtctg
//
LOCUS       DACECJ010000123         4768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.127, whole genome shotgun sequence.
ACCESSION   DACECJ010000123 DACECJ010000000
VERSION     DACECJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.127"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IWO09_002645"
     CDS             25..696
                     /locus_tag="IWO09_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0265206.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IWO09_002646"
     CDS             755..1195
                     /locus_tag="IWO09_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265207.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IWO09_002647"
     CDS             1207..1875
                     /locus_tag="IWO09_002647"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0265208.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IWO09_002648"
     CDS             1939..2562
                     /locus_tag="IWO09_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0265209.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IWO09_002649"
     CDS             2618..3004
                     /locus_tag="IWO09_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0265210.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IWO09_002650"
     CDS             3217..4608
                     /locus_tag="IWO09_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0265211.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1588 a          788 c         1016 g         1376 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagt
//
LOCUS       DACECJ010000124         4754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.128, whole genome shotgun sequence.
ACCESSION   DACECJ010000124 DACECJ010000000
VERSION     DACECJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.128"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1131)
                     /locus_tag="IWO09_002651"
     CDS             complement(<1..1131)
                     /locus_tag="IWO09_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0265212.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDD"
     gene            complement(1261..2637)
                     /locus_tag="IWO09_002652"
     CDS             complement(1261..2637)
                     /locus_tag="IWO09_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0265213.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2637..3293)
                     /locus_tag="IWO09_002653"
     CDS             complement(2637..3293)
                     /locus_tag="IWO09_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0265214.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3303..4580)
                     /locus_tag="IWO09_002654"
     CDS             complement(3303..4580)
                     /locus_tag="IWO09_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0265215.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4599..>4754)
                     /locus_tag="IWO09_002655"
     CDS             complement(4599..>4754)
                     /locus_tag="IWO09_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0265216.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1487 a          866 c          571 g         1830 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgcgcattc tcattagaga acactccctt
       61 ttttgtgact tttctgaagc tagagtgaat actttcaact gcattagtag tgtacatgat
      121 ttttctaatg gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt ttcgtttgca
      181 tacatctact gcaccagaat aatgagacca ccgattttga aaactgtcga aggcagcgtg
      241 tgcagcgttt aaagaagaag cactgtagaa ttttttcatg tctcgacaga cttccttata
      301 atccttactt gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac agcgctgcac
      361 aatgacagaa ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac cgtccatcga
      421 aatgaaaaaa acatcttcta caccacctac tttcagttca tcaaatactt gcatccagcg
      481 atttttagat tctgtttgat tgagccatag gcctaaaatc tccttgtttc ctttaagatc
      541 atagccaaga atggtgtaca cagcatattc tttagcttca tagttttcgc gtaaagtgac
      601 gtacatacaa tcaacgaata gaaatgcata acacttggct aatggccgag cttgccattc
      661 ttccaattca ggaaggacgg catccgtgat atcagaaatc atttcgtggg aaatatcaaa
      721 gccgtaaata tcttcaacgg ttgcggcgat atcgcattga ctcattcctc gtgcatacat
      781 ggaaagaacc ttaccttcga tgtcggagac atctcgtttt ctattaggaa tcaactcagg
      841 ttcaaaagaa gcctcccggt ctctgggaac atcaatgttt actttaccaa agctcgtttt
      901 aagcgtctta ttgccataac cgtttcgtcg attgtcatgt ttctttggct ctttagaatg
      961 ggcgtcatac cctaaatggt tattcaattc tccttgaagc attttttcaa ataagggacc
     1021 aaacacatct tttaaaacat cttgcatatc ctctacagat tcgggttgat aagtattcaa
     1081 aatggattca gctaacttct cagcatcagg atttcttttc tttctagcca tcgtgtaatc
     1141 acctcttgat cttattttag aaaaataaaa accgtgaagc aaccacccgc ctaggattga
     1201 tggttactta cacggtttat attacactct catttttggt gctaaaaagc accgaaaatt
     1261 ttaattattg tcaattaata attcttttgg attccttctt aatatattga atgaagaaat
     1321 aatcaatgat acaagaagta ctaaactcat aaatataact acataaatca tgaatttagg
     1381 taatacattg atatctaaac ttgataatgt cttgttaaat ccttcagctt ctgccccacc
     1441 acctaattgg ctagatgcag attgtttagc tatttgttta gctatatcac cagtaacctt
     1501 attcaatata ttattcccaa gcttgtcagc tgtatattga gctaagaaat aggaaccaac
     1561 gaatgcgggg attgatataa ataccatctc aattaggaat tgaccaaaaa tttctaattt
     1621 tgatatacct aatgatagta atactgctat ttctttcttt ctagcattca tccataagaa
     1681 taataataat gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata atttatttga
     1741 aattgagtaa atacctgata tagattgttg taatgcaggg taatttgatg aacttttaat
     1801 caaattatat tctctccaat ttatatctaa tttcccaaga tcttttatta cactatcaag
     1861 attcttatca ccttttacaa agaatgttgc atcttggtat acagctgtat cttcggtatt
     1921 accataaact ttagcagctg tatgaatatc agtgattaat gtattttcgt atagttcttg
     1981 tgctgcactt actccaccgc tattatgccc atcgaataga cctttaattt ctacttctac
     2041 tgtttcatct gcacctttct cattatcagc atcaaataaa ttagatttta tttttatctt
     2101 atctccaact ttaaggttat ttttttcagc taaatcttta tgcattaaga ttttattttt
     2161 atcttcattt tctaaatgct ttccttctac taatttatat gcttctgata caaattttgt
     2221 ttcttttgtt gagtcgttaa ccccagttaa cataactgtt cttttaaaat tctttgctct
     2281 ttcaggtgat tgattcgcaa gagtctcttg agtttcaata atatcataat caactaaatc
     2341 tgcaacactg tttattcttt ttacatagga gtctatacta tctgtttgag atatcttttt
     2401 aatatcttca ccttttacat ttcccccacc tctaggtgtt cctggattta cttgtctatt
     2461 tatctccata gaaaaactat ttgttatatt agcaaatgtt tcttttgaag ctctgtccgt
     2521 agcatcttta atagataaac taatgagact taaagttgac atagataaaa taactaatat
     2581 gataattaat gattttaaac tttttctagt tacataagca aatgcatttt ttatcattat
     2641 tcaacctcca aattcatttt atttactttt ttcaactttt taccacttag ttctaaaatg
     2701 atatccgcag aatctgctac ttccttacta tgtgttacaa ctattacaca tttatttcta
     2761 tctttagcta atgtctttaa tatattaatt atttctccag cagtaacact gtctagatta
     2821 ccagtaggct catcagctag tattattgga gcatctgata ccaatgctct agcaatagct
     2881 actctttgtt gctgtccacc agataatttc ataacatttc tttttatttg ttttttatct
     2941 aaacctagtt caaacaagat actttcatct gctgatttat ttactaatct aatattttca
     3001 attggtgata aataatctat taaattatag ttttgaaata ctaaagatat attatttttt
     3061 ctatgattac tatatccttt attttgtata tcttcattct taaacaatat ttttcctgtt
     3121 tgaggtttat caagtccagc aagtaaggaa agaagtgtag attttcctgt tcctgacttt
     3181 cctactatcg cataaaactt cccaagttca aatttttgat ttactcctga caaaactttt
     3241 tctttagaat ttgcataact atatgctaca ttctttattt ctaatatatc cattattttc
     3301 tcctaactta tttttgataa gatttcttta ggcttcttga ttaacattaa tgaagaagca
     3361 aatacaactg ataaaacaat aatacttatt aatattaaat aactttgtcc aagtgttgtt
     3421 atatttgaca taaaactact attatttatt aaacttccac cggaaatcat tgaatcctct
     3481 gagttaatga atccatctac aattactttt agtaatacat ttcctaaaaa taaggaagat
     3541 attatacttg gtatcgatat gaatattaac tcgaatataa attgcattat aatttgtatc
     3601 ttagatgttc caatagataa gaatatacct atttcataaa ttctttccct taaccataga
     3661 atcaagatta atgaaagaac aaccatccca cctaacataa tagaataagt cattactttt
     3721 attatatatt ttattccact cactgactct aaagactctt caaacgcatt agaatctttt
     3781 tcaacaaaat actttgactc atcaatttta agctctttca atttgtttaa ggctaagtct
     3841 gtagattctg cactaccaga atacattaaa attttatttg caatttcatt attttctgaa
     3901 ttatttaata tctcttggct tgttgaataa tctacaaaca ccatattttc actaaaatca
     3961 gaagataatc ctgtatatgt ttcctgtttt ttaccagaaa agatccctat aattttaaat
     4021 ttatgacttt ttatttttcc acttttttca atatctagta attcaagatc aacttcatca
     4081 cctaatttta gattgttttg tttagcaaat tcttcatgaa caataattga attcttatca
     4141 ttttcttcta tatttttacc ttctttaatt gtaaatactc cactactaaa taaaatattt
     4201 cttttagtat tatttgtagc ttcgagtgaa acaacattct taaattcgtc agataaatct
     4261 tctctattta ttctttgttc tccgctaact actttagcat cttttagttt tgctaatcca
     4321 tcatattgaa ttattttttc ttcaatttct tttaattttt caatatcttt aaattgatta
     4381 acattaaaat atttaccatc tttttttgtt attgatattg aagaattaga actttcatat
     4441 aaagcctttt ctatttcatt gcttgatttc attattgtta aacaagaata caagcaagaa
     4501 agaactatag ttaatatgat aaaaattatt aaagttctat ttttctttct tgtaatatat
     4561 gctatagcat tttttatcac tttcctaact actcctctct attttcttac tccataacta
     4621 attgcatcca ctggacaagc atttttacat cgtccacatc tgatacactc cagatgattg
     4681 caattctcaa ctgaatcttt attcatttgg caaacttttt tacattttcc acaattaata
     4741 cacttatcct tatc
//
LOCUS       DACECJ010000125         4714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.129, whole genome shotgun sequence.
ACCESSION   DACECJ010000125 DACECJ010000000
VERSION     DACECJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.129"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            108..359
                     /locus_tag="IWO09_002656"
                     /pseudo
     CDS             108..359
                     /locus_tag="IWO09_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="IWO09_002657"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="IWO09_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0265217.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1767
                     /locus_tag="IWO09_002658"
                     /pseudo
     CDS             1656..1767
                     /locus_tag="IWO09_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1788..1871
                     /locus_tag="IWO09_002659"
     CDS             1788..1871
                     /locus_tag="IWO09_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0265218.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="IWO09_002660"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="IWO09_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0265219.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="IWO09_002661"
     CDS             complement(2996..3859)
                     /locus_tag="IWO09_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ0265220.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="IWO09_002662"
     CDS             complement(3861..4133)
                     /locus_tag="IWO09_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0265221.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="IWO09_002663"
     CDS             complement(4150..4365)
                     /locus_tag="IWO09_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ0265222.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4714)
                     /locus_tag="IWO09_002664"
     CDS             complement(4457..>4714)
                     /locus_tag="IWO09_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAQ0265223.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1606 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tcat
//
LOCUS       DACECJ010000126         4713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.130, whole genome shotgun sequence.
ACCESSION   DACECJ010000126 DACECJ010000000
VERSION     DACECJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.130"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(213..443)
                     /locus_tag="IWO09_002665"
     CDS             complement(213..443)
                     /locus_tag="IWO09_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ0265224.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="IWO09_002666"
     CDS             complement(673..1491)
                     /locus_tag="IWO09_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0265225.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="IWO09_002667"
     CDS             1652..2341
                     /locus_tag="IWO09_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0265226.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="IWO09_002668"
     CDS             2355..3857
                     /locus_tag="IWO09_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0265227.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="IWO09_002669"
     CDS             3870..4346
                     /locus_tag="IWO09_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0265228.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="IWO09_002670"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="IWO09_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1590 a          673 c          830 g         1620 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACECJ010000127         4632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.131, whole genome shotgun sequence.
ACCESSION   DACECJ010000127 DACECJ010000000
VERSION     DACECJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.131"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IWO09_002671"
     CDS             174..518
                     /locus_tag="IWO09_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0265229.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IWO09_002672"
     CDS             519..938
                     /locus_tag="IWO09_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0265230.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IWO09_002673"
     CDS             complement(1030..1380)
                     /locus_tag="IWO09_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265231.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IWO09_002674"
     CDS             complement(1346..2566)
                     /locus_tag="IWO09_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0265232.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="IWO09_002675"
     CDS             2932..3498
                     /locus_tag="IWO09_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0265233.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="IWO09_002676"
     tRNA            3643..3730
                     /locus_tag="IWO09_002676"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4632
                     /locus_tag="IWO09_002677"
     CDS             4064..>4632
                     /locus_tag="IWO09_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ0265234.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1682 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat gg
//
LOCUS       DACECJ010000128         4610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.132, whole genome shotgun sequence.
ACCESSION   DACECJ010000128 DACECJ010000000
VERSION     DACECJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.132"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..339)
                     /locus_tag="IWO09_002678"
     CDS             complement(85..339)
                     /locus_tag="IWO09_002678"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0265235.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="IWO09_002679"
     CDS             complement(450..704)
                     /locus_tag="IWO09_002679"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0265236.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="IWO09_002680"
     CDS             complement(897..1172)
                     /locus_tag="IWO09_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0265237.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="IWO09_002681"
     CDS             complement(1144..2097)
                     /locus_tag="IWO09_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0265238.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..4202
                     /locus_tag="IWO09_002682"
     CDS             2709..4202
                     /locus_tag="IWO09_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0265239.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4337..>4610
                     /locus_tag="IWO09_002683"
     CDS             4337..>4610
                     /locus_tag="IWO09_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0265240.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1542 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     4081 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4141 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4201 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4261 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4321 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4381 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4441 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4501 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4561 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DACECJ010000129         4475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.133, whole genome shotgun sequence.
ACCESSION   DACECJ010000129 DACECJ010000000
VERSION     DACECJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.133"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            323..1054
                     /locus_tag="IWO09_002684"
     CDS             323..1054
                     /locus_tag="IWO09_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0265241.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="IWO09_002685"
     CDS             1199..3226
                     /locus_tag="IWO09_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0265242.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="IWO09_002686"
     CDS             3334..4431
                     /locus_tag="IWO09_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0265243.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DACECJ010000130         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.134, whole genome shotgun sequence.
ACCESSION   DACECJ010000130 DACECJ010000000
VERSION     DACECJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.134"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWO09_002687"
     CDS             complement(543..2048)
                     /locus_tag="IWO09_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0265244.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWO09_002688"
     CDS             complement(2045..2731)
                     /locus_tag="IWO09_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0265245.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO09_002689"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO09_002689"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0265246.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACECJ010000131         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.135, whole genome shotgun sequence.
ACCESSION   DACECJ010000131 DACECJ010000000
VERSION     DACECJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.135"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWO09_002690"
     CDS             690..1658
                     /locus_tag="IWO09_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0265247.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWO09_002691"
     CDS             1669..2484
                     /locus_tag="IWO09_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ0265248.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWO09_002692"
     CDS             2552..3262
                     /locus_tag="IWO09_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0265249.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWO09_002693"
     CDS             3298..>4425
                     /locus_tag="IWO09_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0265250.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACECJ010000132         4312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.136, whole genome shotgun sequence.
ACCESSION   DACECJ010000132 DACECJ010000000
VERSION     DACECJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.136"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IWO09_002694"
     CDS             complement(<1..281)
                     /locus_tag="IWO09_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0265251.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..1272)
                     /locus_tag="IWO09_002695"
     CDS             complement(730..1272)
                     /locus_tag="IWO09_002695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265252.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(2049..2239)
                     /locus_tag="IWO09_002696"
                     /pseudo
     CDS             complement(2049..2239)
                     /locus_tag="IWO09_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2359..2721)
                     /locus_tag="IWO09_002697"
     CDS             complement(2359..2721)
                     /locus_tag="IWO09_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265253.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="IWO09_002698"
     CDS             complement(2725..3033)
                     /locus_tag="IWO09_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265254.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="IWO09_002699"
     CDS             complement(3042..3461)
                     /locus_tag="IWO09_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265255.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="IWO09_002700"
     CDS             complement(3454..3687)
                     /locus_tag="IWO09_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265256.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4312)
                     /locus_tag="IWO09_002701"
     CDS             complement(3684..>4312)
                     /locus_tag="IWO09_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0265257.1"
                     /translation="SQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1221 a          959 c          618 g         1514 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aa
//
LOCUS       DACECJ010000133         4202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.137, whole genome shotgun sequence.
ACCESSION   DACECJ010000133 DACECJ010000000
VERSION     DACECJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.137"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..353)
                     /locus_tag="IWO09_002702"
                     /pseudo
     CDS             complement(1..353)
                     /locus_tag="IWO09_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(613..1161)
                     /locus_tag="IWO09_002703"
     CDS             complement(613..1161)
                     /locus_tag="IWO09_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265258.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="IWO09_002704"
     CDS             complement(1162..2019)
                     /locus_tag="IWO09_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0265259.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="IWO09_002705"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="IWO09_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="IWO09_002706"
     CDS             complement(2581..2910)
                     /locus_tag="IWO09_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0265260.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="IWO09_002707"
     CDS             complement(2987..3259)
                     /locus_tag="IWO09_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0265261.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="IWO09_002708"
     CDS             complement(3259..3522)
                     /locus_tag="IWO09_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0265262.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3628..4044)
                     /locus_tag="IWO09_002709"
     CDS             complement(3628..4044)
                     /locus_tag="IWO09_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265263.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4050..>4202)
                     /locus_tag="IWO09_002710"
     CDS             complement(4050..>4202)
                     /locus_tag="IWO09_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0265264.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1593 t
ORIGIN      
        1 aaatgttctt actgcctctt ccacactaat ttcttcgttc aatttcacta ataatacttg
       61 atctcgctta ttataatcgc tttcatttat cctaagagtc aaatcttttt ccaccataac
      121 ttttacaccc aataaatttt gcggtttatt tttctctgtt gaaattcttt gtgtagcctt
      181 taaactgaca attagctcat tgggtacata cttatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatttttat taacaagttt
     3661 tatcacaggc ttcatatatt tgattataac ttcatcttct acgtagatat ctgttatcat
     3721 tacgacatct gtactatttc gattgagtac tttcacttga tctccagcat tgattttccc
     3781 tctaaatctt ttaggaatga accaactata catatgatta gcatcaccaa aatgtgcttt
     3841 tatcgcattc tcaggatata acttattcaa tagttcattc tttttaattt tcaaaacttc
     3901 aaatctatac ttatcatcat ctgctaatct aaataaatct tctaaacgca tgttttctaa
     3961 ctgtaagttt tgaaaatctt ccggtataaa ttttcggttc tgttgcgaag tttgaaaatc
     4021 aaacgcgccc tctctgaact ccaaatatct taggctgtta ttcccattaa taccttgatt
     4081 tcagtagaca ccgaaaagct gaagagcgtt ccatttcttc ggttcttttt atatattcct
     4141 cgaatggtct ccatgccctt aatcgtggaa gaggctgtac ggagactttg ataaaattta
     4201 tt
//
LOCUS       DACECJ010000134         4031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.138, whole genome shotgun sequence.
ACCESSION   DACECJ010000134 DACECJ010000000
VERSION     DACECJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.138"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            848..1060
                     /locus_tag="IWO09_002711"
     CDS             848..1060
                     /locus_tag="IWO09_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265265.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1338..3137)
                     /locus_tag="IWO09_002712"
     CDS             complement(1338..3137)
                     /locus_tag="IWO09_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0265266.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3261..3857)
                     /locus_tag="IWO09_002713"
     CDS             complement(3261..3857)
                     /locus_tag="IWO09_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0265267.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1447 a          711 c          689 g         1184 t
ORIGIN      
        1 aaatattata cagataataa cggaatatta tcatcgaaac agctaacaaa attgtcataa
       61 aaaacgagat ggttgatatt agaacttgtg tttttgttta ggtctagaga taaagagaga
      121 caatttccaa atatatgtaa tgaatacaaa aagacgtttg tcgcggatta ccgttacaaa
      181 cgtcttttat tgattttccg agatctcatc agtcgccatt tggaatggtt cactccttat
      241 ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa taccaaacgc aacgttattt
      301 tcgttttgtg catcaatcgc cataaaacgg ttagaagttg tatcaaaagc tacgcggtat
      361 tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct ttgtaatgta atttagttct
      421 tgaaagtttc ctttaaactt tcgaacttat accaagttta gcatcgtacg ttttttgatg
      481 caattaaaaa gcatttatca ttttccttat ttattttttc ttaaatggtt tagctatttt
      541 cttttaatcc gataagtagc ccaatcatca aggttacaaa tactattgct tgaatataaa
      601 tcatatgctc acctctttat aaataactta gcaatataaa gaaaaaaacg cacgaacaac
      661 ataggaatat aaacaaacgt ttttgtcaca acgtctaaga tcgtgttaaa aaatagttat
      721 ctaaaaagca gaaagatact caaaataaaa aataaaaaaa tggataaaaa cgttttcaaa
      781 tacaaggtaa tttgttatta taaaataaag aaaaaacaaa ataaaaataa agaaaagagg
      841 gaatgaaatg gtaaaagcag aagtaggaag atcttatata tgcaaaccga tcggagtatc
      901 tcatgaacta atcggctgta ttgaacgtac atatatcaac acggctgtcg tgtatgttga
      961 aagttatgac caacgagact atcaacttat caatgaaagc aagtatcgca tgctcgtaaa
     1021 attctgtgat attagcgcac ctgctgaatt ggtttcttaa acagtatttt tgagaattcg
     1081 caagaactcc cttataatat aaataaagac agaagggagc gagaagatat gccaaaaaga
     1141 aaaagttcag ggaaaatggg aattattctt gcagtgatct ctgcaattcc tgctatttta
     1201 gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag cacacattat gagcgtcaaa
     1261 caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt ttgttgtata aagacttttt
     1321 tcattaacga tttttgatta gccgactccc aaaacaaaat ttagcagttt atctgaataa
     1381 gtgatttctt catctgtttg tttcacactt tcatttagta gtcccatctc atcggttatg
     1441 ataccatcca caccataaaa catcatgcga ctcatagcat ctgaatcgtt gattgtccat
     1501 acatatactt cttttccgtc ttggtgggca gaattaatga agttacggtt gatcgtacta
     1561 tattccattg tcagaaaatc agcaggcgtc acagggggcc caacaatatt gaaaggtaag
     1621 atgtagccca cataaaagtt tggttctgct tcttttaaat ctgaaacggt ttggtacgtc
     1681 aaactttgca aaatatgatg gttctctaag ataactttgc ggtatttatg gacaaatcgt
     1741 tcgacaaggt catccgaatc ttttttagag gtcttgatct caataagtaa tttttggttt
     1801 agcttatgtg ctttttccaa ataagaatca aaagaacaaa caggggcttg cactccgttt
     1861 tcagtaactg taagatttgt caattctttc aatgtaagat cctgtggtgt tccatttacc
     1921 cctgttaagt gacgaagatt tgcatcatgc atcactacga aatgaccatc ttttgtttcc
     1981 tgaacatcca tctctacata atcaggatga tagtctctgc ttgttttttc aagtgcttct
     2041 aaagtatttt gcgccccatt ttgggtactc acacctctgt gagagacagt cactggtttt
     2101 gtctgaacag ctgaagtcag ataatccata ttgtaaagac agacgccaat accgaaaaaa
     2161 actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga aacgcaagtt gggggcttgt
     2221 gggataaacc acttagggat ctcaggcaag aagccttcgt catccatgaa atcaacaatg
     2281 atataaaaaa tccctacggt agacatcacg atattgaata ataaaatccc ttgtaaaagc
     2341 gtcatagcaa acacagcgct gatcaatgca tagtctggga aaaattgttc aaccatactt
     2401 tgtgccaaaa taacaacgat atatcctaaa gaagaaagga gtaaaatcgt accgccaatc
     2461 acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg tcaatgacca gctttcccga
     2521 atggctgctc gaaaaggacg atctcgtaga atcatttccg gtaaagcaaa aatcagtcga
     2581 atcccaatat agccaaggaa aatataaacg agtaaaaagg aactgacgat gatccaacga
     2641 ttagcaaaaa taaaatccat aatgaaagcg gggattttta tttttgacag tagatcagaa
     2701 ttaaaactta atccgctgat cggtagaatc aataaaaaat aagcaaggaa aaacaaaaaa
     2761 gtaatgggtc tcactttctt cagctgaagt atcgtcaagt gtaagagttg cttcaaagag
     2821 attggttctt tctttttaat aaaaaaaaca ctcatcaaaa gaaaagtaaa ttcgaaataa
     2881 actaacagta aaatcgtcat caaaactacg attaacgaga gtagaacccc aggatgttga
     2941 gaaaaaattg aaggaatatt gtcataagac aaatagtcaa ttgcccctcg gtgtaaaata
     3001 aaacgggtcg tactgctcaa taagggtaaa caaataaata aaataaagcc atgcatcagc
     3061 aagacatcac ggaaataagc ttgtgttcct tttaaaaagt cccaagcatt acctaagcta
     3121 cgtttcaaat aattcatcct atcacctgct cttcacattg tagcgcaaat ctaatgaaaa
     3181 aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat ttaacattgt caatcaatga
     3241 ctaattttac aaaataaata ttatccaaga agaccactta gatattgacc tccataaatc
     3301 aaggccatac tatataagaa aatcgacagt ggtttagcaa tcaaaatgat tagtgtatat
     3361 cgtttcactg acatgtttgt caatccagcc atcaaacata acgcatcgtc aggagcaact
     3421 ggaagaaaaa tagctagagc aaacagcttc tcaaaacggt tttgattatc cagccagcca
     3481 atatatttgt tatatgtctt atcgctaatc atggaaagta tgaatggctt tccataacga
     3541 cgccctagca gaaaaatgat aatcgaacca atgcagattc caatatagtt atagataaat
     3601 ccagcataag ggccaaatat caggacacca gctgcagtac tgatccctcc aggaatgatt
     3661 ggaataacta cttgcaggat ttggatgaag acaaaaataa tcggtcctaa aatgatggaa
     3721 tcacctacta atcctcttaa cgcattcaaa tctttgaaaa cacctaaatt aataaaataa
     3781 atcgtcagcg cgattgatag gcctaatccg ataatggata tgaaatttat cagttttcga
     3841 gaggcagcaa tattcattat tcatcaactc cttagaggtt ctttgttaac tatactttac
     3901 tagaaaagca actaactgcg ccattttttg atatgacaga aaacaaacaa ttttgtaagt
     3961 tcatcataag tataaaagat tgatctcaaa aaaacatcca actaaagact tatttaataa
     4021 cttgtttttt t
//
LOCUS       DACECJ010000135         3976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.139, whole genome shotgun sequence.
ACCESSION   DACECJ010000135 DACECJ010000000
VERSION     DACECJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.139"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..1484)
                     /locus_tag="IWO09_002714"
     CDS             complement(18..1484)
                     /locus_tag="IWO09_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0265268.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(1775..3859)
                     /gene="cadA"
                     /locus_tag="IWO09_002715"
     CDS             complement(1775..3859)
                     /gene="cadA"
                     /locus_tag="IWO09_002715"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0265269.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1203 a          846 c          633 g         1294 t
ORIGIN      
        1 taacaagtat ttctatttta aggctgaacc actgtttctg gttcagtgat ttttttgttt
       61 ttgtctcgac gagcaattgc tggcaagatc atacctaata agatcaagac gattggtgtg
      121 atgatattcg taaataattg gaaccaccat gctttaggat cagcagtata atctactttt
      181 ggcaccattc ctaggataca tgcaaacgca gtgaaggcaa agcaccaagc accaaagata
      241 aaaccgattt ttggattttt aacaaattta tattcagaat tgaatttctt ataagcttta
      301 ttcaacatca tatatgccaa gaacacccat aggtaacgca ttggcatgac aactgagttc
      361 aagttcgtca accattttac taattcattc atatctttga taccaaaaat cggcaacaaa
      421 ataatgattc ctactagtat acctgtcaga agataaccat tcaccaatgt acctttacga
      481 ttacgtttac gtagccagtt tggtacaaat tctggatccg catctgctaa caagattttt
      541 agtggtgcat caattgaaaa ggctaatgcg gagatttgtc caatcatgtt tgtcaatgcg
      601 taaatgatca tcaatgagtt accgatacca tagtaattac ctaaaatttg aaaagcactg
      661 tatgccccat ttgccatcaa gtctttagga atattatcac tagcaaacag catacccatt
      721 gcgactgagc ctaatacagc acaaatccct accattgctg ctaagaagat catcccttta
      781 ggaaattcct ttgcaggatt tcttgtagag ttgacataag gggatatttt ttccgcccct
      841 ccaactgcaa atactagcat cgaaacggtt gtgaaataac tgaaatcaaa tgttggaata
      901 tatgttttca cttgattcat atgtggtgtg gcaaattcca tcgttggatt gatgaacggt
      961 gccgttacag ctaaaagaat aaataagatc gacatgacga acatcgctgt tccggccagt
     1021 ccgccaatca ctttcaaagt agaaagtcct tttgttgata aatataaaaa ggcaagaaat
     1081 ataataagtg aaataactgc taccatttgt actgacatcg tatttacgat atttccattt
     1141 ccttggactg cccaaccacc agcaatcaga attgcttgag gcttttgtgc caaataagga
     1201 atatggacga cccagtaagt ccaagcggca tagtatgcca gtcgtttatt agatgtcgta
     1261 ttttcgaccc atgaactgac gcctccttta ctatccttga aggctgatcc taattgtcca
     1321 acgatcaatg cgtatggaat aaaataaatg gccaaaatca atagccaaga ggtgacaact
     1381 gatactcctt gctgagcata gttattaact acattcccca tgccccaaac catgttaaat
     1441 gcaattaacg caacagtaaa ccagcgtaat tgtttgttgt tcattcgttc tctcctttat
     1501 gataaaaatt tgttatccag atgtaatcat attcaatttt taacaaactt acaagacagt
     1561 gttattttat gtgtgtattc ttttatttaa tacacatcgt tttttcatat tttttccatc
     1621 aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag ctattttgaa
     1681 ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc tttacatttt
     1741 tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctctttt tttacatgca
     1801 agcttcgcat ggcattcaaa acagctaata ctgtcacccc aacatctgca aatactgcat
     1861 ctcccattga tgcaaaacca aaagcaccta aggcaagtac caaaattttt atcccaatag
     1921 caaagacaat gttttgtttg acgattttca atgttttctt tgctaaatgg attgcttctg
     1981 caatcttcga tggttgatca ttcatgatga ctacatctgc tgcttcaatt gcagcatcgc
     2041 ttcccaatcc acccattgcg attcctacat ctgctcttgc tagcaccggt gcatcgttga
     2101 ttccatctcc gacaaatgcg gtctttttat tgtttctttg taagatttcc tctaaccgct
     2161 gcactttatc ttcaggaagc aattcgctat atatcttgtc cacgcctact tgcttcccga
     2221 tatgatcagc aatttttttc gagtcgcccg tcaacataac tgtgttcttc acaccagcct
     2281 gttttaattg aaccaatgcg tctacggcat cttctttcaa ggtatctgca ataacaagat
     2341 aaccactgta actttgatcg atcgctagat aaagcaatgt accgatttct tgacttaaag
     2401 gaaaagagat ttcaaatctt tccatcaatt tgtgattacc tgctaatact tgtttgccat
     2461 caacttcagc taagatccct tcacctgcaa attcctggat attagttgtt ggtaaaagcg
     2521 gacctgcgta gccttctaaa acagattgag caatcggatg tgtagaaaac tgttctgcac
     2581 tcgcaatata ttgcatgaat ttctttggat cgatttttgt atcgatcgtc tggatcgaaa
     2641 agtcaccttt cgttaatgtg ccggttttat cgaataccac tgtctcggtt tgcgcaagca
     2701 actccaagta atttgaacct tttactaata caccgatttt acttgcgcct ccgataccgc
     2761 caaagaaact aagtggcacg gaaatcacta atgcacatgg acatgagatc actaaaaatg
     2821 tcaatgctcg atagatccat tcagaaaacg ccgctccaga aacaacaagt ggaggtaaaa
     2881 cagctaataa aacggccaat cctacaacga ctggtgtgta atagcgcgcg aatttagtaa
     2941 tgaaattttc agctggtgct tttttactgc tggcattttc tactaattct aataatttgc
     3001 tgatagtaga attttcataa gatttttcta cttgaactaa aaatggttta tttttattga
     3061 taaatccgct taatactgtt tcaccaacat atacagattt agggactgat tctcctgtaa
     3121 gtgctgacgt atcgactaat gattctcctt cgatgatcgt tccatctaac ggtacacgtt
     3181 caccaggctt cacaagtaca aactgaccga tctgcacgtc ttcagggttc acggtcatca
     3241 atccttccgt cgtctgcacg tgagctgttt ctggacggat cgccatcaat tcgcgaatcg
     3301 attttcttga atgactgact gccaatcctt ggaaatactc gccgatttga taaaatagca
     3361 tcactgctac ggcttctgga tattcaccga tcaacatagc gccaatcgta gcaacagaca
     3421 taagaaaatt ctcatcaaag atttttcctt tgacgatatt catcactgcc ttcttcacta
     3481 catcatatcc tatcagcaga taaatcaata aataccctat caagcgaatt ggttcattaa
     3541 aatcaaataa tagcaataaa gcgatacctg caagggaaaa taaaatccgg aacagcgtat
     3601 ttctctcttt ttcatttcca tgttcagata ctttttcctt atctatttct gttactttga
     3661 catctggttc taattccttc actacttttt ttgtttcttg ttcaatcgta gccaaatgat
     3721 cttcctctgc gtcaatcgtt agttttccag aagtgaagtt taccgttgct tccttgactc
     3781 cattgatctt ctgaactcct ttttcgattt tcattgcaca gttcgcacaa tctaaacctt
     3841 ctaatctgta acttttcatt ttaatgacct cctcgcctca acatatgaac atctcttcat
     3901 aactaattat aaatgaatgg ttattcatat gtcaaggtat ccgaaagaaa aaatgaaaaa
     3961 catctgcatc ggaaaa
//
LOCUS       DACECJ010000136         3717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.140, whole genome shotgun sequence.
ACCESSION   DACECJ010000136 DACECJ010000000
VERSION     DACECJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.140"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..2542
                     /locus_tag="IWO09_002716"
     CDS             101..2542
                     /locus_tag="IWO09_002716"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0265270.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="IWO09_002717"
     CDS             complement(2872..3576)
                     /locus_tag="IWO09_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265271.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1193 a          654 c          813 g         1057 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaa
//
LOCUS       DACECJ010000137         3712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.141, whole genome shotgun sequence.
ACCESSION   DACECJ010000137 DACECJ010000000
VERSION     DACECJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.141"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWO09_002718"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWO09_002718"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0265272.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IWO09_002719"
     CDS             complement(2260..2511)
                     /locus_tag="IWO09_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0265273.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IWO09_002720"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IWO09_002720"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0265274.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IWO09_002721"
     CDS             3461..3679
                     /locus_tag="IWO09_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265275.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DACECJ010000138         3473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.142, whole genome shotgun sequence.
ACCESSION   DACECJ010000138 DACECJ010000000
VERSION     DACECJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.142"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IWO09_002722"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IWO09_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IWO09_002723"
     CDS             complement(545..739)
                     /locus_tag="IWO09_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265276.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2091..3131
                     /locus_tag="IWO09_002724"
     CDS             2091..3131
                     /locus_tag="IWO09_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0265277.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT         1293 a          476 c          494 g         1210 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgt
//
LOCUS       DACECJ010000139         3162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.144, whole genome shotgun sequence.
ACCESSION   DACECJ010000139 DACECJ010000000
VERSION     DACECJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.144"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1125
                     /locus_tag="IWO09_002725"
     CDS             <1..1125
                     /locus_tag="IWO09_002725"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0265278.1"
                     /translation="IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1115..2332
                     /locus_tag="IWO09_002726"
     CDS             1115..2332
                     /locus_tag="IWO09_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0265279.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2345..>3162
                     /locus_tag="IWO09_002727"
     CDS             2345..>3162
                     /locus_tag="IWO09_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0265280.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
BASE COUNT         1089 a          534 c          658 g          881 t
ORIGIN      
        1 atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat cggggatgcg
       61 tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg agaattttac
      121 acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca aaaggaacga
      181 cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt
      241 aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa tacaacgaca
      301 tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga aatgaatctc
      361 cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac atttgatgca
      421 gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt cttagatgat
      481 tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt tgcgtttctt
      541 cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt accacacgga
      601 gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga agatggcagt
      661 atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat tccaacgacg
      721 gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga tgctagtcgt
      781 gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag aaaacattca aaaaatcctc
      841 gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc atttggcaac ctttgatgaa
      901 atcaaagaaa atgactataa cttaaatatc ccacgctatg tagatacttt cgaagaagaa
      961 gaaccaattg atatggttca tgtcggaaat gacatcaaga agatacgaca agaacagcaa
     1021 gtgctagaaa aaaaactttt agaagctatc tcttccctgc aaacaacacc tgaaaatgaa
     1081 gcgtggttac aaggtgcgct ggaggtgttc aaacatgagc aatgatacac aaccagaaat
     1141 ccgtttccca ggatttacag atgattggga acagaggaag ttgggggact tcgttgtaga
     1201 ttatgttgaa aagacaagtg tacaaaatca atttcctatg ctaacttcat ctcaacaaaa
     1261 agggatagtt ttgcaagaag actattttgc aaatcggcaa gtaactacag aaaataatat
     1321 tggttacttt gtactaccta gaggatattt cacgttccgt agtcgaagtg ataatgatgt
     1381 gtttgtgttt aaccgtaatg acattattga tagagggatt ataagctatt tttatcctgt
     1441 atttacttta aaatctgctg attccgactt cttcttaaga cgtattaaca atggaatcca
     1501 acgacaatta tctattcaag cagagggaac aggtcaacat gttctgtctc taaaaaagtt
     1561 taaaaatata gtagcaatgt ttcctagtga ggaagaacaa caaaaaatcg gcaccttctt
     1621 caaacaactt gacgacacga tcgctcttca tcagcgtaag ttagatttgc tgaaggaaac
     1681 gaaaaaagga ttcttacaaa aaatgttccc tgaaaacgga gcaaaagtgc cagaaatccg
     1741 gtttcctggt tttacggaag attgggaaga gcgtaagttg ggagaattag gtaaaactca
     1801 atcgggaatt ggatttcctg atgccgagca aggtggttct gaaggtactc cattttttaa
     1861 agtttccgac atgaataaca ttggtaatga gtatgaaatg agaaatgcaa atcattatgt
     1921 atctaatgag caaattgagc gtaaaaaatg gaagccaata aaagatgtgc cagcgattat
     1981 atttgctaaa gttggcgcag caataatgct taatagaaaa cgtttggtca caagcccttt
     2041 cttaatagat aacaatacca tggcttattt atttaataat acttgggata tctattttgg
     2101 taaaatattg tttgaaacta taaatttgcc aagatactcc caagtagggg cattgccttc
     2161 ttataacagt tctgatattg agaatatcag tgttaaggtt cctgtaaagg atgaacaaca
     2221 aaaaatcggt actttcttca aacagctaga tgatacgatc gctcttcatc aacgtaagtt
     2281 agatttacta aaagaaacga aaaaaagctt cttacaaaag atgtttgtct aggagggaag
     2341 aagaatgaaa attagtcacc gtgaggaagc agaagtcgaa gaacagttga ttcgtgtact
     2401 tggagaaggg cataatcagt ggacgtatcg tccggatcta aaatcagaag aagacctttg
     2461 ggtcaattta cgccaaaaaa ttatctcaaa taatcaagca gaactgaatg attttccttt
     2521 gaccgataaa gaatttgaaa cgatcaaaac ggaattactg ctgcgaacga aaaccccatt
     2581 tgatgctgcg aaatggttaa aaggtgaaaa tgggatggct cggatcacga ttgaacggga
     2641 agattcgcaa ttgggttctg tctcacttat cctttattcc aatcaagaca ttggtggagg
     2701 aatctctact tatgaagtgg tccatcaaat tgcaaaacgt ggaagtaaca tagaagcacg
     2761 ggatcgacgt tttgacgtca ccttattaat caatggatta ccgattgtcc aaatcgagct
     2821 gaaacaagta actgcaaaag atggtgttta tcaggcattt aaccaaatca aaaaatatgc
     2881 agaagaaggt atgtttcgga acaatatctt ttcgacctta cagttgtttg tggtttccaa
     2941 tgaacaaacc actcggtatt ttgcaaatgc cttaccgaaa gacttgcacc ctaagttttt
     3001 atttagttgg cgaacgaaag acaatgaaaa agtagaaaac ttatatgagt tttgtaaaca
     3061 agtgttaaat atcccggatg ctcatcgttt gattgcggat tatacaatcg tcagtgaaga
     3121 tcaagacaat aaaaccctca tggtgttaca tccttatcaa gt
//
LOCUS       DACECJ010000140         3024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.145, whole genome shotgun sequence.
ACCESSION   DACECJ010000140 DACECJ010000000
VERSION     DACECJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.145"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1243
                     /locus_tag="IWO09_002728"
     CDS             <1..1243
                     /locus_tag="IWO09_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0265281.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="IWO09_002729"
     CDS             1256..1888
                     /locus_tag="IWO09_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0265282.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="IWO09_002730"
     CDS             2093..2320
                     /locus_tag="IWO09_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ0265283.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="IWO09_002731"
     CDS             2389..2655
                     /locus_tag="IWO09_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0265284.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="IWO09_002732"
     CDS             2645..2914
                     /locus_tag="IWO09_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0265285.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DACECJ010000141         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.146, whole genome shotgun sequence.
ACCESSION   DACECJ010000141 DACECJ010000000
VERSION     DACECJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.146"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IWO09_002733"
     CDS             177..359
                     /locus_tag="IWO09_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ0265286.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IWO09_002734"
     CDS             462..746
                     /locus_tag="IWO09_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265287.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IWO09_002735"
     CDS             762..998
                     /locus_tag="IWO09_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265288.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IWO09_002736"
     CDS             1020..1274
                     /locus_tag="IWO09_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265289.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IWO09_002737"
     CDS             1356..1541
                     /locus_tag="IWO09_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ0265290.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IWO09_002738"
     CDS             1608..2003
                     /locus_tag="IWO09_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ0265291.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IWO09_002739"
     CDS             1993..2370
                     /locus_tag="IWO09_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265292.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IWO09_002740"
     CDS             2371..2766
                     /locus_tag="IWO09_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265293.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DACECJ010000142         2992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.147, whole genome shotgun sequence.
ACCESSION   DACECJ010000142 DACECJ010000000
VERSION     DACECJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.147"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(106..1053)
                     /locus_tag="IWO09_002741"
     CDS             complement(106..1053)
                     /locus_tag="IWO09_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0265294.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IWO09_002742"
     CDS             1299..1763
                     /locus_tag="IWO09_002742"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0265295.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IWO09_002743"
     CDS             complement(1825..2868)
                     /locus_tag="IWO09_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0265296.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          989 a          593 c          571 g          839 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa cattttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACECJ010000143         2987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.148, whole genome shotgun sequence.
ACCESSION   DACECJ010000143 DACECJ010000000
VERSION     DACECJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.148"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..272)
                     /locus_tag="IWO09_002744"
     CDS             complement(12..272)
                     /locus_tag="IWO09_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265297.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            347..550
                     /locus_tag="IWO09_002745"
     CDS             347..550
                     /locus_tag="IWO09_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0265298.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(691..1176)
                     /gene="ssb"
                     /locus_tag="IWO09_002746"
     CDS             complement(691..1176)
                     /gene="ssb"
                     /locus_tag="IWO09_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0265299.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1179..2078)
                     /locus_tag="IWO09_002747"
     CDS             complement(1179..2078)
                     /locus_tag="IWO09_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0265300.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2091..2684)
                     /locus_tag="IWO09_002748"
     CDS             complement(2091..2684)
                     /locus_tag="IWO09_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ0265301.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
BASE COUNT          801 a          571 c          484 g         1131 t
ORIGIN      
        1 cagctcttct tttatatgcc catcgatcgc gtaaaatgat cggggtttct aatttgcatc
       61 gattcaatga ctaattgatt ccgtttattg aaatgacctg tgacggcaat attgtatttt
      121 ccatcatcca taattaacaa tttatcgata atttcaatat ctgcaactac acaattaatc
      181 gattcatgaa ttgtttgtaa agtaaagcga accaataatg gttgtgtttt tattactcgg
      241 attttatcaa gcaaaccaac atatttgttc atcacgatca cctcgctaaa attatagcaa
      301 aaacagctaa aattgagaat ttttattcga ttttagatat gctataatga tcaagacagt
      361 aaagtttgtg acggtggcta tccgattgaa agtgaggtgg tgcgtatgtg cgtacttcca
      421 aaatcttaca gaaaggagaa gccttttgcc tgttgaaacg gcgctaggat taatgattag
      481 tttcggttct ttgatcgcaa caatcatttt tggaatctta gcagttgtaa acaacaatca
      541 aaaaaaataa ccgtcagctt tagccggtcg acgattattt ttcaaaaata atattaagct
      601 ggtcaccgtc ttaaacggtg ctgttagggg agttgttagc gcaactcctc ttttcgtatc
      661 cattataaca actttattga tcttttacaa tcagaatggt aaatcatcat cactaatatc
      721 gattgattgt ccgccaaatg gattttgtga agcgttcaat ccattgttgt taggcgtagc
      781 tacatcccgt tgttcaaagg tggtagggct tggactgctg gacgattgat cagcgtgcgc
      841 acggctttct gtcgctgctt tactttctaa tagttggaaa ttatcggcca caacttctgt
      901 cacatacaca cgttgtcctt gttggttatc gtaggttcgg gtttgaatcc gtccaacaac
      961 gccgatcaaa ctgccctttt tagctaatgt cgccatcgtt tctgctggtt tgcgccaaat
     1021 cacacagtta ataaaatcag cttctcgatt gccgttttga ttcgtgaaat ttcggttgac
     1081 agctagggta aaagtcgcta ctgcagtacc actcgcggta taacgtaaat ctggatcttt
     1141 ggttaagcgt cctactaaag ttacattatt gatcatcttt atctctccat ttcttgattc
     1201 atttgttgtg atcttttaaa ctgccgtttt ctttcttgat tttcaatttt gatcacactt
     1261 ttcattaggt gatcaatttc gttgactgta tctgtaaatg aaatggaatt cgcaaacgtg
     1321 ttaactgagc gatatttttc ccactgcttt tccaaatagt catttcctct gatcttatcc
     1381 atttctcttt cataataaaa ggacaacggt tttgtatccc cacgttttgt aaatgtattt
     1441 cgaacacttt tgtataactc tttcgtatcg atattttcta gtttcggaat ggtaaataag
     1501 tcgtagtaat cttttgctct cgaattatct gtgccaaagc tgaacgtagc acttagcttt
     1561 tctgcaataa tttgttctgt tggataagct ggaatctcca cttttttatc ttcaaacatt
     1621 aattggtgat agtgtgtatc tacttcagga taaatagaat ctcctgttgt cacgtccatt
     1681 ttaaacggta cacgtacttt ctctaaatta gccaccaatt taatttgaaa tccaggataa
     1741 aaatcggctt ctcttgtttc cttcattcct tgaatagaga aaacaatgcc ttcttttgtg
     1801 ggttttctag ttaaatcatt aaaaatgctc ttcaattttt cttcagttaa tttgctattt
     1861 ctaaatgtcg tatcgatatc aattgttgtc cttctatcga ttccatattt tgatcctaat
     1921 aaaaagccgc ctttcaaaat gaagtcattt ttgtactttg attcagaaat ttgttcaatt
     1981 agacgatcca tcataaaatg ccgttgcact aactgtgcat ttaatcctgt actctttgct
     2041 aaatttcttg tttttgctgc aaattgtgtc ggtgtcatct ttttcctcca tcaatataag
     2101 ctttgcatgt ggtatttcat ttctttttct acatgtagtt ttgtcatgta atctcttaat
     2161 ttacttggat ctctcaatgg gtcttccatg tactctttta atgcttctaa tttcatttca
     2221 tattcaaatt catatctcgg attccaaata tcacaaagtg tacgctcttt atcatacata
     2281 gtaataggat ttccttgaaa ggaagttccc tctacttttc ctagttcata aacttctttc
     2341 ttcacgaaat gaaattgtaa ctgctgctct tttgtaattg aacgatcaac gttatagcca
     2401 tggggaaaag ttaagtgaat gaatttcggc atattcgtag aaagctggta aatatctaaa
     2461 gtcgtttcat gagagaagat tcccctggaa aatcgttgct gaagccaaaa taaatcatct
     2521 attccatgac catcctctac ataataacca cgatcaactt tgatcaattt tccttgattc
     2581 accaatcttt gtacagtggt tgctgcgatt ccaaatttcc tggcttcctt catagataaa
     2641 tttccatcgc gaatagccaa caattctttc agtttatctt gcatttactt cccctccctt
     2701 cgttacaaat acactacatc aaatatatca tagtagttta tttgtaacaa gattaacgtc
     2761 tatcgatcta attcgatttc tttttgaatg cgctgctggt tctttaattg ttgcagtctt
     2821 cttttatttg cacgtaattt ctcttgttct aaacgaattt ttgctttttg attttcgatt
     2881 tcttgttgtt tgtgtgttgt atttcgcata ggataacccc ttcattattt tgtagtgcgg
     2941 cgtgttcttt tcttatagtt cgtttttttc ttttcagaaa attcaaa
//
LOCUS       DACECJ010000144         2860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.149, whole genome shotgun sequence.
ACCESSION   DACECJ010000144 DACECJ010000000
VERSION     DACECJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.149"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..499
                     /locus_tag="IWO09_002749"
     CDS             <1..499
                     /locus_tag="IWO09_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAQ0265302.1"
                     /translation="YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIK
                     QPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEF
                     VYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCAN
                     ASEAV"
     gene            600..1172
                     /locus_tag="IWO09_002750"
     CDS             600..1172
                     /locus_tag="IWO09_002750"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0265303.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1528..2100)
                     /locus_tag="IWO09_002751"
     CDS             complement(1528..2100)
                     /locus_tag="IWO09_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0265304.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2116..2721)
                     /locus_tag="IWO09_002752"
     CDS             complement(2116..2721)
                     /locus_tag="IWO09_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ0265305.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          893 a          570 c          471 g          926 t
ORIGIN      
        1 ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc attttggatc tgttcaatcg
       61 agaaattctc ggctattctt gtggagaaca taaagatgcc gttctagtaa aaaaagcatt
      121 tagccgtatc aaacaacctc tgacagaggt tgagattttt catactgatc gtggaaaaga
      181 gtttgataac caagctattg atgaattatt aacaactttt gacatcaatc gatcattgag
      241 tcataaaggc tgtccttttg ataatgccgt agctgaatca acttataagt cgttgaaagt
      301 agaatttgtc tatcaataca catttgaaac cttacaacaa ttggatttgg agttatttga
      361 ctatgtcaat tggtggaacc accttcggtt gcacggtaca cttggctacg agacaccggt
      421 tggttaccgt aaccagagat tggcgcagcg aatccttgat aatgagctcg gatgtgctaa
      481 cgctagcgag gcagtctaac tttaactgtt agctcctgcc gaagatcgtc acatccgagg
      541 aggctcattg tcaaggacaa tcggaatagc atacggaaag aagttctgca ccttataaaa
      601 tttgtcaaaa aaactgttgc cattccatca tttcaaaaga gaatctctta aatcaggact
      661 tttctactga aacgatctgt gagaaatggg cagctgacat tacgtacatt cctactaaga
      721 aaaatggttg gtgttactta tcttcaatca tggatttaca cacgaaaaga attattagtt
      781 atacattttc aaaacgaatg actgtggatt gtgtcattca aacattgaat aaagcaaaaa
      841 tacattatca cattccagaa ggaatgattc tacacactga cttgggcagc caatacacag
      901 caagagaagt ggaacaatgg cttaaaacca acaaaataag gcattcttat agtagaaaag
      961 gaacacctta tgataatgct ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag
     1021 tctacacgac tagctactca gattttgaag aagcaaatcg agcgctattt agctacattg
     1081 aaggatttta taaccgaaat cgaatccata gctcgattca ctatctaacc ccacaggaat
     1141 ttgaagaatt agcaaaagaa aaaatggctt aaccgtctct actaaagaca atcaacaccg
     1201 tagggatatg gagataaaat caatagagtc aatagattat ttatcgataa taacactaaa
     1261 aaagccacgt tcctaaacgt gacttacgct gaacgatatg tgttgtttgg tagacgaaca
     1321 aatcgttcat tataaatata tgatggtatc ctaatatagt aagcgtggct agcatacctt
     1381 taattacttt accttttaca gttaccatac acattttttg agcagaaatc aagtaaataa
     1441 aaaaatatta tatttcacaa catgctaata gactcatatc tagaacgcat taagtattta
     1501 cctgtctggt tcgctgagac ttctttttta actttcttca attttcttct ttattctaaa
     1561 aagcgtactg cgactgaaac cggttatagc ttcaacttcc ttatacgatt tacctgaagt
     1621 aagcagttca tatgcgtgcc tcttcttcgg tgtgattgtt gctttcggac gtccttctct
     1681 gaaattagga ttattctttc ttgcaaacat ttttccttct tgggtacgtt ccacaatcat
     1741 atcacgttcc atttctgcta cactcaacaa cgtccgtgtc accattcgcc ccatggacgt
     1801 attttcaatc gttcctagat tcaaaacctt gatcgttacg ttatcatcga gtaaaggttc
     1861 aatgatctct aatgcttctc tcgtgttacg agcaaatcga tcaagcttcg taacaactat
     1921 ttcatctcca ggcttcagac cagccaacag ctgattgaat tttggccgat cccgttttgt
     1981 acccgtaaat ttttctgaaa aaatattctt cttctctacg cagttctcaa gcaactgttg
     2041 aatttgttct tttgtacttt gagaagcggt tgagacacgt gcatatccat atttcgtcat
     2101 ttggtccacc tacttttaca aatcatctat cgtatactca gaataccctt catagaaata
     2161 gctgacatcg attcctttca tatataactc gcgatcgtca attttatcag tcagagcatt
     2221 ggaaattaga tagcgtattt ccaaatcgtt tatgggactt cgctccattg cagaaagata
     2281 gtctgcttta tcgattaagt tccaatctac aactttttgc aattcttcct ttaaaatgag
     2341 atccagccaa attctagtac ttcggccatt tccttctcga aatgggtgag cgatattcat
     2401 ctccacatat ttcttgataa tctcttcaaa agaagtttgt ggcatctgat caatatgctg
     2461 taacgaatgt tctaaataca taactggtgc aaatcgaaaa tttccttttg caatattgac
     2521 cgatcggatt tttccggcaa aatcgtaaat atccgaaaat agaaattcat gaatttcaga
     2581 taaaccttta aatgtaccaa cttcgatttc atttattttt ccacaatcgt acaattcttt
     2641 cgctttttgt ttactgagtt tttcttcaac cttagccaac tcaacttgat tggttaatcc
     2701 caatttattc tctaaaatca tgcgaaatct ccttaacttt atgacataag tttatgaaac
     2761 actctcaacc ccattatatc gcgattttgt ttatgtgtca aaaactatta agtttctgac
     2821 ccccgataga tttttcaagt gtctctattt tgcacagtct
//
LOCUS       DACECJ010000145         2825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.151, whole genome shotgun sequence.
ACCESSION   DACECJ010000145 DACECJ010000000
VERSION     DACECJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.151"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            798..1064
                     /locus_tag="IWO09_002753"
     CDS             798..1064
                     /locus_tag="IWO09_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265306.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1122..1634
                     /locus_tag="IWO09_002754"
     CDS             1122..1634
                     /locus_tag="IWO09_002754"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0265307.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="IWO09_002755"
     CDS             1631..2668
                     /locus_tag="IWO09_002755"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0265308.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          495 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaaca
//
LOCUS       DACECJ010000146         2766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.152, whole genome shotgun sequence.
ACCESSION   DACECJ010000146 DACECJ010000000
VERSION     DACECJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.152"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            120..470
                     /locus_tag="IWO09_002756"
     CDS             120..470
                     /locus_tag="IWO09_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0265309.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            884..1702
                     /locus_tag="IWO09_002757"
     CDS             884..1702
                     /locus_tag="IWO09_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0265310.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1775..2134)
                     /locus_tag="IWO09_002758"
     CDS             complement(1775..2134)
                     /locus_tag="IWO09_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0265311.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          934 a          391 c          489 g          952 t
ORIGIN      
        1 tatgcttcta tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac
       61 tctccttgta tgctttagcg ggtattacaa tttgagtgta acataaatgg cttttttatt
      121 tgtctcgctt aattatacaa acggcgtttt ataaagcaat cattcctgag aacttagatg
      181 aggttaaact ttatttatgg gaagataacg gagaaattgt cggttttagt ggtgtaagtg
      241 cagaagaatt agttatgcta tttcttgatc cagattttat tggtaagcga tatggtagtc
      301 aaatattaat tgagctgatt gaaactgaag atataaaaag aattgatgtt aatagtcaaa
      361 ataaatatgc aaaacatttt tatttaagcc atggatttga aattgaatct gaagaagact
      421 tagatggttt tggtaaacca taccctataa cgcatttagt aaaaaaataa ttcatgaatg
      481 gtttattacc tgattgaaca tgaataaaag taatattttt attttgatgg cctatttcta
      541 ggttatgaat tatccagatc aaatttgtgt attacattca gtaataaggt gcttatctta
      601 ggatcaactt atgatactag ctgaccaagg atgtaccagt tatagaaaat cagttaaagt
      661 aaaaatgata aaaatcttat gtcaacactg atttaggctg acctgtgggt taggagaaag
      721 acaggtcggt agcttttaat acaaaaaatg gcttgtatgt atctagatag ttaactgaaa
      781 ttagaaatgt tcttattttt aacggagtaa gaaaaatagg ctgctttagg tctgaatact
      841 ttttcaatat aatgaagata tcaaaggcga caggagtgtt tttatgaata agaatcctca
      901 taatctaaag ttggtagcag tagatatgga tggaacattt gctcgtactg attacacata
      961 cgatatccca aggttcaagg ctgttttatc gcgaatgaaa gctgttgatt gtcaatttgt
     1021 tgttgcaagt ggaaatcaat actaccaatt gagatcgtta tttccaaatt attatgatga
     1081 attaagtttt gttgctgaaa atggtgcttt tgttaaagat aaaacagagg tactattttc
     1141 agccgatatg cctagaaaag cgatcgaggt ggtccttgat ttatgtaaag actatcctga
     1201 agtaaaaaat gtgatgtgtg gtctgaatag tgcctactgt gaaagaggaa aggtgagtca
     1261 agcatttttt gatctaacca atatctatta ccatagatta gaatgggtag ctgattttgc
     1321 agaagttcaa gataaaattt taaaatttgc tccgactatg cctgttgaaa aaaccgaatt
     1381 ttacgttgaa ttgttccgtg aaaaattagc cgggctatta gtacctacca gtagtggtca
     1441 tggttctgta gacttgattc tttctgagtg ccataaaact ttcggcttga aaatgttagt
     1501 tgagcgcttg gggattaatc ctgaccagtg cgttgctttt ggagatgggg gcaatgacat
     1561 tgagatgttg gagtattgtg gtctgagtta tgaaatggat aatgcaacgg aagctgttaa
     1621 gcaagttgct aagcatcaat gtccttcaaa tgacgaagac ggtgttttag tgacgctcga
     1681 tcgattgttc cctaacacgt aaacactagg taattcatca aaaaacctcg atataagaaa
     1741 tattcgaggt tttaaaacta tatttagaac agatttacga agcgtctgat gagtcaaaat
     1801 agacttggac attgctatct aaaataccac tttcattctt taaacgaatt gtttgatttg
     1861 atggagttac ctctaatact aaatgcccat taataaaggt agtatcgtat tcagatgttt
     1921 taactgttgc ttcttcgcaa ggtattgtta aatcatatgc tggaatataa tgttgtgtgg
     1981 aaactaataa aaaatgatca ccaatcatac agcatccctc tgctgaagaa aattgattag
     2041 agccatcatt taaagttaac aagaatccat cctttaaacg aggtacttgc ttaaatactt
     2101 ctggttgtac ttctaaaaat aaattatcgc tcattttcac gaccaccatc gacttccaca
     2161 gttgaaattg acattttatt taacacacct attccccctt aactataact caattctata
     2221 taaaaaatat tctattctca aataattgaa cttttcaaat cgccaattta cttcggcttt
     2281 atcagagctc attcatatca agagcgatta acgctgtaaa aattaaaccg aagatctgat
     2341 aaattttctc tattttctac aatatttaaa acgcttcctt agtctattat atatctattt
     2401 ctcttaggag tatgtttaga tgtttgaata atagttgccc aactttaaag gttattctta
     2461 taatatttta cttcattttc caatagaaaa ttcaaaggat tactggtttt tctaaaatag
     2521 tttagtttaa atacataagt atcctccgac actgtattag ttcataatca tctaaataca
     2581 aaattttcaa ataaaaatat ttcaagctct atcgttgacg aatgtaaacg atacttataa
     2641 agtaatagaa attgattaca tttgtaaact taaaatggtt aatttatctc acataaaact
     2701 aagaataaaa taatgattaa aagtggttag tttttatacg atttagccta tcggtaaaaa
     2761 tgaatt
//
LOCUS       DACECJ010000147         2735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.153, whole genome shotgun sequence.
ACCESSION   DACECJ010000147 DACECJ010000000
VERSION     DACECJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.153"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWO09_002759"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWO09_002759"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0265312.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1070 a          425 c          558 g          682 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atcca
//
LOCUS       DACECJ010000148         2724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.154, whole genome shotgun sequence.
ACCESSION   DACECJ010000148 DACECJ010000000
VERSION     DACECJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.154"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..387)
                     /locus_tag="IWO09_002760"
     CDS             complement(<1..387)
                     /locus_tag="IWO09_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007892530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0265313.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSP"
     gene            complement(436..1368)
                     /locus_tag="IWO09_002761"
     CDS             complement(436..1368)
                     /locus_tag="IWO09_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0265314.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(1789..2649)
                     /locus_tag="IWO09_002762"
     CDS             complement(1789..2649)
                     /locus_tag="IWO09_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0265315.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          792 a          550 c          422 g          960 t
ORIGIN      
        1 tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt gaagaaccta acgtgccata
       61 agacgctccg gtaatcatca ctggtttatc cacaaacgga aaaataccat atgaaagcca
      121 atttaatgcg ttaatcaatg aagcaggaac cgcatggtca tactctggtg tgctgatgat
      181 tacgccatct gcttgttcaa tttttccagc aatttcttta acaacatttg gtagttctgt
      241 gttttcaggt ttgttaaata gtggcagctc cttgatttcg acaatttcga tttctgcctc
      301 atttacaaaa taatttttca taaactgtaa taattcacgg tttgttgacc gcgttgaatt
      361 tgttcctact aatccaataa atttcatctt cattctcttt cctttctaac atcgatttct
      421 gactgttcgc acgtactaaa ttttttcaaa tccttttgta tacaagaatt gattctcttt
      481 tgttacaact gctccctcga tacccggctg ttcttcaagc gctttcaaca cttctaaaga
      541 agatgctcca aataaacgtg tagtccagat ttctccatct acagaagaat cagacaaaat
      601 cgtcaggctt gctacgtcat ttttaactgg aaagccagtt ttcggatcaa tgatgtgatg
      661 atacactgac gaaccatttt gtagataacg ttcgtagatt ccagaggtaa ctaaagattg
      721 gttgtttagt ttaaccaccg caagcgactc ttcacgtgaa cctttaggat ttcgcaaacc
      781 aatacgccaa gcaccatccg aacgtcttgg tgattcacca aaaacaacga tatttcctcc
      841 taagttaact aaagcaaacg ctgcatttcg ctctcggcaa tgtgtaatta atttatctgc
      901 aatgtaacct tttgctaacg cccccaagtc aatcatcatt cctggtttgg ttaaaaaaac
      961 ggttttcatt ttttcatcta attgaatttg gtgtggatcc gttagcagaa gtaactcagt
     1021 tatctctgct tttgttggaa ccaatgcatc ttcaaatcca atgtgccatt tttgtacaac
     1081 cggaccaata gcaatattca gataactatt tcttgcacaa ctatgtgctt ttcctaattt
     1141 gatgagctca aaaagttcag gatgtacaat cacagcttgt cgtccagcag cttgattgat
     1201 ggccattaat tcagaagttg catcatgtgc gctgaagcga tgttcataaa cgtgcaacca
     1261 ttcgcctaac tctgctagta atcgactagg ctcttcatgc ccaacttgta atgtaattgt
     1321 tgtccccatc atttgccaga ttttcgtttc ttcgatcacc tgattcattt tgtaccttct
     1381 ttacttgaac tacggattac atgagtaatt agtttttatt tttgttactg ttttcacaac
     1441 cagatgatac gcctgctaga tttatttgtc aaccgatttt atatattttt ttatataata
     1501 aggtaatttt aatataaaat tttatattgt aaaaaaacaa ataagggaaa caaacaacac
     1561 atttttgtgt gttgcttatt tccctctaac tatcgttttc tatttgatgg ttaacctact
     1621 taaaataaat actgcaacga caaacggaga tcaagcatgc tttgtaacgt attttacgat
     1681 aattacgact ttattttatt actctatctt gctgccatac ccgtctatcc ctaatcatct
     1741 ttggtgaatt ttttcaaagt tagcataaca aaaataagta ttttttcctt agttaatcaa
     1801 tagtatcatt tttttcatat gatttaattc atcaattaat tttttgtttt tattaatttt
     1861 agcaaaagct agtgcatctt gaatcaattc gagcacttct gccttcgact tatttttagc
     1921 ctgtgcgttc ttcgctaatt gggcagcagc tcgtgctaag taataagtca aatggttatc
     1981 cgaacaaata tcgattgcgt aatttagtag tgcatcactt gtttccaaat caccgtcttg
     2041 cgcaaaaaaa acaccacaat gaaagacgat atttaacacc cgccagatat cttctgttgt
     2101 acgaatcgga tacgtataaa ttttttcaaa cactcgattg aaataaactt ctgctttttc
     2161 caactctttt tctctagcat agaccattcc aatacctgta tatgccaata aacgaaacaa
     2221 cgtgttggct gtttctgcca tcaacaactg atcaaaatgg aacaacacat ccgtaattgg
     2281 tgcattcgta aaaatcattt caaatccttt tagatagtag taacgtgcaa tcagctcaga
     2341 ctcgtcaatt accttgggat caatggctgc taaaatctgt gtcaattgtt catattcact
     2401 cgtaatcagt gaaaactctg ctttttctag ctgttcatca agttgtgtat tgatcacgtt
     2461 catcgaagaa aaaagttcaa ctaacggaag attgattcga ttacataatt tgattaaaat
     2521 ttttaacgaa gggacttgac cattgttctc aaatttactt aatgttgctt gtgtacaaat
     2581 tccttcagac acttcttttt gagataagtt ggcagccttt cttgccgtaa taaatttttc
     2641 aatgttcatt attttctctc caatcagacc tcacacgaaa atcatttctg tctcgatttt
     2701 agcatgtttt ctttgggaat gctt
//
LOCUS       DACECJ010000149         2702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.155, whole genome shotgun sequence.
ACCESSION   DACECJ010000149 DACECJ010000000
VERSION     DACECJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.155"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..2492)
                     /locus_tag="IWO09_002763"
     CDS             complement(21..2492)
                     /locus_tag="IWO09_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0265316.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
BASE COUNT          779 a          554 c          473 g          896 t
ORIGIN      
        1 cttttttgag atacttttcg tcaattttct cgtttaaata tcgatagaaa ttttttagct
       61 acgcgactaa acggcttttg ctctttttca tttttacgtt caaacaaata ttctaccaat
      121 gtggtcaaat gcctaaaaat cgaatctgcc gagaaaaata gaatgctcaa cagcaaaagt
      181 gatacagctg agataccggt gattgaaaac agcttatgca aaaagatgac cgaaaaaaag
      241 ttaccgctag cacagaaaag aatgatctga cttctgaatt tattcagcgg tgaacagatt
      301 ttatagagaa cgaggaaccc tactgcaatc aaaagcatgg tagatgctgt agaaacatct
      361 gttttattta gattcaaagt caccccgcag atcaccaacg caccgactac gatcatatct
      421 gttagtccac ctggcacagc tttttccatc acattcatga tgaatttccc tctgattcgc
      481 tttttgtttt cttctagagc aagcaagaaa gatggaagtc cgatagtaaa caaactaatc
      541 aatgtgattt gtgatggttc taaaggatag gtcagagcaa agatcactga aaacagtgag
      601 agcaaaaagg agaaaatatt cttcactaaa aacaagctgg ctgatcgctc aatattgttc
      661 acaacgcgcc gtccttctgc aacaacttct ggcatgcgtg aaaagtcaga atcaagtaag
      721 accacttgtg aggcctgcat ggcggcttca tttccggaag ccatcgctat actgcagtct
      781 gcttctttca tggctagaat atcattcacg ccatcacctg tcatagctac tgtattgtta
      841 cgctttatca attgaacgaa gattttcttt tgttctggct tgacgcgtcc aaaaactgta
      901 tatttttcga cagctgcttc atattcttct tctgataatt tagatgcatc aacgtactga
      961 tcagcatgtg aaatccctgc ttggacagct acattggata cggtgagcgg attatctcca
     1021 gaaatcactt tgatggctac ttgctgtttt ttgaagtaat caaatgtggc tcttgcttct
     1081 tttctgatag gattagcaat cagaatatag cctaatggtg tgactgcttc tgtcaatact
     1141 ggttcttcaa gaatcccatc gtacacacca aagaccaaca cgcgatagcc tttttcagcg
     1201 aaagaaacaa actcttccgc atattccggc aattgatcgc gcaaaaccat ttcgggcgct
     1261 cctaaaacat acaccttgtc tgccaacgct acactgctga atttttggac agaagaaaaa
     1321 ggaataacat ggtgatagga ttgatcagtg gtttttgtaa aatatgcttt aacagcttcc
     1381 attgttgcat tgtctcctgc catcgctttt gcatagtcag caatgcatgc ttccaattga
     1441 tctgactgac tgattatttc ttgtttttta gagacgatca ttttttgaac agacatttta
     1501 ttttctgtga tcgttcctgt tttatctaca caaaggacat ttacccttgc caatgtctca
     1561 atgcttttca tattgtgtaa aagaactctt tgctttgcta gtttagtcgc acttaatgct
     1621 aatgcaatcg tcgtcagcaa gtacagtcct tcaggtatca taccgatcaa tgctgcttcc
     1681 attgcaacga cactttcttt gatcgtgttt ccattaaaaa agtaactttg agaaaaaagc
     1741 aggataccga taggaataat gatgatccca atccatttga tcaattgatt aagagaagca
     1801 atcatctccg actgttctcc ttctcccatt gtcttggctt tgactgtcag ctgggaaata
     1861 tacgaatctg ctccaacttt gtctaatcgg gcgtaagctt ttccggaaac aatgaaactg
     1921 ccggacatta atggatcacc tatttctttg gttatttcat ctgcttcgcc tgtcaataaa
     1981 gcctcattaa cttgcagttt cccttccacg acctctgcat ctgctggaat ttgatatcca
     2041 gtctctaaaa taacaatgtc atctattaca aggtcttcta tatctattgt ttgttctatc
     2101 ccgtctcgta tgacattgac ttttgctgta tttaaaacat ttagttcgtc caagatcttt
     2161 ttggatcgaa tttcctgaaa aatggcgatt cctgtattgg ctatgataac tggtaaaaaa
     2221 gtcaagttct tataggattc tactaaacaa agcaaaatgg ctaagataag aaagatcaaa
     2281 ttgaaatacg taaatatgtt ttccaatacg atttgctgat ttgttttgaa tgtgggatcg
     2341 actgaattat ttgctgcacc tttttctatt cgttccttga cttgatctgc cgttaatcct
     2401 tctttatacg atggaaaaag acggatgact ttatctgatg atctttgtgt tactttcttt
     2461 tttttacttc gatttatcca tttattcttc atgtggctac ctcgattgat ttcgttttct
     2521 ggatatttcc aaacttctcc tatccagtat agaacagata tggaaagtgt tcctcacatt
     2581 aagcttgcaa acacctttca ttcctgtcat atagcaaaaa aaaagggttc tataatattt
     2641 tgtgttggtg gaaaatcgaa ggcgattttc tactaacact ttttcttcgt ttcaactcaa
     2701 aa
//
LOCUS       DACECJ010000150         2633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.156, whole genome shotgun sequence.
ACCESSION   DACECJ010000150 DACECJ010000000
VERSION     DACECJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.156"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..402
                     /locus_tag="IWO09_002764"
     CDS             <1..402
                     /locus_tag="IWO09_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0265317.1"
                     /translation="DEIIASVNYLKKKNVKLIITSLPIMAEAIGNPLLDKFIKDLIIQ
                     ILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRLVYKSIVEDLK
                     NGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(567..1391)
                     /locus_tag="IWO09_002765"
     CDS             complement(567..1391)
                     /locus_tag="IWO09_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265318.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(2029..2286)
                     /locus_tag="IWO09_002766"
     CDS             complement(2029..2286)
                     /locus_tag="IWO09_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0265319.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2279..2548)
                     /locus_tag="IWO09_002767"
     CDS             complement(2279..2548)
                     /locus_tag="IWO09_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0265320.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          865 a          465 c          328 g          975 t
ORIGIN      
        1 gacgagatca tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact
       61 agtttaccga tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat
      121 ctgatcatcc aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc
      181 caggcacaag gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta
      241 tacagtgcag acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat
      301 cttaaaaatg gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc
      361 gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta
      421 ttatcttctc atctttaatt tcctacatat aattactatc tataaaatta agtcttactc
      481 agaacagata aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct
      541 gttaatataa tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct
      601 atattttcta caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc
      661 gacacattgg ttatactaat tcctatagct agttcttgag actctacttt tctctctagc
      721 tcctttgaca aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct
      781 tcataaaaat ctctatacat aaagctatca ttaattttat aaaaaaaatt aaatgttttt
      841 agcactgcca tataacattc aaaacattct tctatagttg gtttacttcc ttgaggagaa
      901 tgaaccaatt tattacgttt ttctatgaaa tttttaattt cctttcttat ttctttcgga
      961 aactcaatat taaagtaccc tttaaacatg ctccctaata atttgtctac agggggagat
     1021 tgtaaattct caaatattag agttaaatca ggtttctctt gttgataaaa ttcctttacc
     1081 cctatttcac aggctgtaat tatcgaaata aacctatctc tagtatttga tatattttta
     1141 cttcttttaa ggagtataag agaaaagggg atactttcgt gtccatgcat gtaattaact
     1201 aattgttgag cttcagaagt tatatctcta attatatagt taacggaatt aggaagtgtc
     1261 tttaactcta atgaaatttc tatccaatta ctatcttcta atctgtatat gctcgaagta
     1321 taatttgatt cgtcagcaag catcgctaca tcccaaacac tgatcataaa atcttttaaa
     1381 cgttcaatca tttcagcaat attgctaaaa tcagctaatt tttctttaga ataaaaaaaa
     1441 catttatatc agaatcaata aattctattt tttttattac tatatcgtca tcattatctt
     1501 gaccatttat gtttattgtt ccctgagtgg ggagtacttc tttcaaattc aagttaaatt
     1561 tatattccat tacttctcct ccaatatata aaaattactt aactgtatcc aaatttaata
     1621 tgtaaataaa tcattaattt ttagctaaat tctatgagaa gtttattttt gttgtttttg
     1681 ctaattaact aaaactagta caaaaaattt aaaatagatt attaaaccta ttgcttattc
     1741 tattcagtca tatatataag cgtcatcctt tagtaactaa catcccacac tacaatagta
     1801 ttataacata tctattcaac ttcaaattgt tgaaatttaa gccatttata tttacttttc
     1861 ctcaacttta gaaatgattt tactaacctt atatccgtaa tacgcgtaac aaacacattc
     1921 attaataaca atcaacacta agctatatta gctttttaaa tgactagggt aaataaaata
     1981 aggatcaaaa acttttaagt ttctgaccct ttccttactt ccgattggtt aatagtgatc
     2041 ttttgcagaa taaataaata tcgtttcatt ttcaactcta tatatcagtc tatgttcatc
     2101 tgtaattctt ctggaccatt ttccagataa atcatgcttt aatggctcag gttttcctaa
     2161 tccagcaaag ggggaacgat cgatatcttt tattaacttg ttaatctttt ttatattgct
     2221 tttgtttcct tgctcatgcc aataaagata atcatcccaa gcatcatcag accaagcctt
     2281 aatcatcaga ttcaacctcg attaagtcat gtgttttaaa tgcacctttg gagaattgtt
     2341 catctcctcg acgaattttt tccatgacgt aattattaga aagtgttctc aacgtttctt
     2401 gcatagaatc ataatctctt tttgataata caacaactgt atcttctaca tctttacttg
     2461 ttacaataag tgtttcagca tcctcattaa cttgtttcat ataactacgt aaattttggc
     2521 ggaaatttga ataagctact gcttccattc ctttcacccc ttatttattt ctataaaaca
     2581 attgtacatt atattgtaca attaagcaaa tgattaatat tctaacttct tat
//
LOCUS       DACECJ010000151         2574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.157, whole genome shotgun sequence.
ACCESSION   DACECJ010000151 DACECJ010000000
VERSION     DACECJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.157"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..1253)
                     /locus_tag="IWO09_002768"
     CDS             complement(join(91..977,977..1253))
                     /locus_tag="IWO09_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0265321.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            1528..2070
                     /locus_tag="IWO09_002769"
     CDS             1528..2070
                     /locus_tag="IWO09_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265322.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            2160..>2574
                     /locus_tag="IWO09_002770"
     CDS             2160..>2574
                     /locus_tag="IWO09_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ0265323.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          848 a          433 c          492 g          801 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgccgactg agttgaagcc cacgctttgc
      781 taaacacttc ttcaattttc gggtaccgta agcctttcgg tttcgaataa actcttcctg
      841 aacgatctct tctaagtcgg actcattttc gatcggtttc gcttgataat agtaggtctg
      901 acgagaaata tttagaattt tgcacatcgc tgatatggaa tatttatgct tgttggcatc
      961 aattacttgt cttttcgtcc gaatatcagc gccgcttgct ttaaaatatc attctccatt
     1021 tcgagttgct tatttttctt tcttagtgcg atcaattctg cttgttctgg tgttaagttg
     1081 tctctttctt tgaatgagcc cgttgattgt gcttgcttca tccatttatc gaaagaagaa
     1141 ggcgtaagct catactctcg aataatttct gcgcgaggct taccagcgag atagagatcg
     1201 acgatttgtt gtttgaattc ttttgaatag gttcttcgtt gacgtcttga catgaaaaat
     1261 cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt ctagtgtagc
     1321 cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt cgtaagtgtc
     1381 cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt gtaagaacaa
     1441 ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa attatatttc
     1501 atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa atgctattgt
     1561 gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc atgcctatta
     1621 cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga aaacaataaa
     1681 ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa tgcaaatggc
     1741 aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga atatgtttat
     1801 gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat agctgataaa
     1861 aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta tgctattttg
     1921 acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat gggcgatgga
     1981 tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt tgaaaaaaat
     2041 acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa ataggagttt
     2101 caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa agtaatcttc
     2161 tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt cctaaaaatg
     2221 gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac aaagaatcgg
     2281 ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt acaagtaaag
     2341 attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt acagcttgtg
     2401 taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag ggtgtaaata
     2461 tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa gcaattgaaa
     2521 agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg ccag
//
LOCUS       DACECJ010000152         2485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.158, whole genome shotgun sequence.
ACCESSION   DACECJ010000152 DACECJ010000000
VERSION     DACECJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.158"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            280..486
                     /locus_tag="IWO09_002771"
     CDS             280..486
                     /locus_tag="IWO09_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0265324.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            486..737
                     /locus_tag="IWO09_002772"
     CDS             486..737
                     /locus_tag="IWO09_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265325.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            752..1189
                     /locus_tag="IWO09_002773"
     CDS             752..1189
                     /locus_tag="IWO09_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265326.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            1182..1889
                     /locus_tag="IWO09_002774"
     CDS             1182..1889
                     /locus_tag="IWO09_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265327.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            2270..2449
                     /locus_tag="IWO09_002775"
     CDS             2270..2449
                     /locus_tag="IWO09_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265328.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          904 a          412 c          520 g          649 t
ORIGIN      
        1 tgcacgttca ctaagatatg gtgtcttgca aagcaagaca aaatagaaaa aacttcctct
       61 caccaaaagg ttcgttcctg ttcgctatct ctttattgat ttgccagacc ggcaacaagt
      121 tgccttttat caccaaaaag cgacaaggac aagggtaaat ggcacgctgc gcgcccttgt
      181 ccttgtcgct tttcgttgat tcattctggc caacaaatca ataaagaaag aactaacgaa
      241 aggaaataaa acctttccta agaggaggca gaatcaatca tgcgaaagaa atttgattat
      301 tggggtgttc ccttcagcga attattccca aactatcatg caccacatac tgtcgaatgc
      361 gattgtggag aaagagcgaa atgtatcaaa agctatcgtt tgtatcaatg tccaacatgc
      421 gggaaaaaat ataccttgtc ttatggcgat tatgttttga ttgatgagaa agtaaagaaa
      481 cggtgatgaa aacacaacac aaattattga ctgtaaaaga agtcgttcaa attttggtgg
      541 acaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
      601 tagaaacccc tttagtagag gatgcaccga ctatgctatt tgaagaccta gtgaagattg
      661 caggatatga actgcattat caagaaaaca tggaaactta tgaaatcgaa tattggttaa
      721 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcaa aattactatg acgaatttgt
      781 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
      841 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
      901 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacactgg aagaactgaa
      961 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagtag gtgtcaaacc
     1021 aagcactatc aaaccttccg aatatcaagc cttagaactg acttatgcaa aattcgcaca
     1081 gattaagaaa gctaaagcaa tggctagcga ctggctagat acttttgaaa atatcgaaaa
     1141 aaatggtgca ttttatacga ttgaatcgga ggagaaagat aatgaataag gtggtttatc
     1201 agctaacaac agaagatgtt tcgcaatttt taacaaacaa agaacacttg aatgaagtaa
     1261 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
     1321 tggaagaaca gttgtatatg gaaagtgaaa cgagtcaaac gaaggttaca gtcagccata
     1381 atcgtttaga cgtgtataaa cgtcgtcctg acacattaaa aaaaggagcg gagcttctac
     1441 gactagaaac agaaaaatat atcatgaatg tttacctaga aaggagtgag ctggtcgaaa
     1501 actttgtcag ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa
     1561 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaagatgcga
     1621 cggtagacga ttattacctt gtatttacta gatttttgac gtgtgggacg gaatctatcg
     1681 tgttaatggg aatggattat gaactcactg tacccgaagc atttttgagg gaatatgtag
     1741 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagacacct
     1801 acacttacga cgatacagaa gaaatccaaa atgaacttga ccgtaaacga atgccatatc
     1861 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag gatgtgtgaa
     1921 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgatccaa
     1981 ccgcccccgc cgcttaggtg gtgcgaaata agcgggcaaa gcccgataac ctaagagaaa
     2041 gaaggagatc catatgaata aaatcattcc tctattgttc gtactgtttg ttggcgagat
     2101 acttatgcgt catggtgcag ctctacattt aaacttcgta gtattcttag gaatactggt
     2161 attttgtttg atcttttggc gctttgtgtt aacccttgtg agtctaatat tcaagttgat
     2221 tgcagccata tttttaatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
     2281 aataggaaat aaacggttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagca
     2341 actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
     2401 gattgcccaa gaaaaagcag gatttgaggt accagcactc aaaaagtgac gtcacttttt
     2461 atcctaaaaa ctaaaagtga tagca
//
LOCUS       DACECJ010000153         2473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.159, whole genome shotgun sequence.
ACCESSION   DACECJ010000153 DACECJ010000000
VERSION     DACECJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.159"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWO09_002776"
     CDS             7..624
                     /locus_tag="IWO09_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265329.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..>2473
                     /locus_tag="IWO09_002777"
     CDS             1644..>2473
                     /locus_tag="IWO09_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0265330.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          905 a          326 c          397 g          845 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt tta
//
LOCUS       DACECJ010000154         2454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.160, whole genome shotgun sequence.
ACCESSION   DACECJ010000154 DACECJ010000000
VERSION     DACECJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.160"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWO09_002778"
     CDS             132..344
                     /locus_tag="IWO09_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265331.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWO09_002779"
     CDS             506..1024
                     /locus_tag="IWO09_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAQ0265332.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWO09_002780"
     CDS             1031..1543
                     /locus_tag="IWO09_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265333.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="IWO09_002781"
     CDS             1814..2437
                     /locus_tag="IWO09_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0265334.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaa
//
LOCUS       DACECJ010000155         2448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.161, whole genome shotgun sequence.
ACCESSION   DACECJ010000155 DACECJ010000000
VERSION     DACECJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.161"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1364)
                     /locus_tag="IWO09_002782"
     CDS             complement(45..1364)
                     /locus_tag="IWO09_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0265335.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IWO09_002783"
     CDS             complement(1361..2014)
                     /locus_tag="IWO09_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0265336.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2448
                     /locus_tag="IWO09_002784"
     CDS             2302..>2448
                     /locus_tag="IWO09_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0265337.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEH"
BASE COUNT          815 a          379 c          224 g         1030 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacac
//
LOCUS       DACECJ010000156         2408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.162, whole genome shotgun sequence.
ACCESSION   DACECJ010000156 DACECJ010000000
VERSION     DACECJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.162"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(243..464)
                     /locus_tag="IWO09_002785"
     CDS             complement(243..464)
                     /locus_tag="IWO09_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265338.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="IWO09_002786"
     CDS             complement(562..807)
                     /locus_tag="IWO09_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265339.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="IWO09_002787"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="IWO09_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="IWO09_002788"
     CDS             complement(2031..>2408)
                     /locus_tag="IWO09_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ0265340.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DACECJ010000157         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.163, whole genome shotgun sequence.
ACCESSION   DACECJ010000157 DACECJ010000000
VERSION     DACECJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.163"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWO09_002789"
     CDS             5..1150
                     /locus_tag="IWO09_002789"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0265341.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWO09_002790"
     CDS             1321..1779
                     /locus_tag="IWO09_002790"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0265342.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IWO09_002791"
     CDS             1938..>2407
                     /locus_tag="IWO09_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0265343.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACECJ010000158         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.164, whole genome shotgun sequence.
ACCESSION   DACECJ010000158 DACECJ010000000
VERSION     DACECJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.164"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWO09_002792"
     CDS             207..2069
                     /locus_tag="IWO09_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0265344.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IWO09_002793"
     CDS             2069..>2398
                     /locus_tag="IWO09_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0265345.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACECJ010000159         2319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.165, whole genome shotgun sequence.
ACCESSION   DACECJ010000159 DACECJ010000000
VERSION     DACECJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.165"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            123..515
                     /locus_tag="IWO09_002794"
     CDS             123..515
                     /locus_tag="IWO09_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265346.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="IWO09_002795"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="IWO09_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="IWO09_002796"
     CDS             857..1054
                     /locus_tag="IWO09_002796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265347.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="IWO09_002797"
     CDS             complement(1097..1672)
                     /locus_tag="IWO09_002797"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0265348.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1868..2302)
                     /locus_tag="IWO09_002798"
                     /pseudo
     CDS             complement(1868..2302)
                     /locus_tag="IWO09_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          743 a          399 c          356 g          821 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgcta
//
LOCUS       DACECJ010000160         2203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.166, whole genome shotgun sequence.
ACCESSION   DACECJ010000160 DACECJ010000000
VERSION     DACECJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.166"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..605
                     /locus_tag="IWO09_002799"
     CDS             <1..605
                     /locus_tag="IWO09_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0265349.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..2082
                     /locus_tag="IWO09_002800"
     CDS             739..2082
                     /locus_tag="IWO09_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0265350.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          877 a          269 c          394 g          663 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttattttaa aggcagtaac aggattaaac attgaaacat aatcgttact aaatgaaatc
     2101 aagaaatata ctgtattact caattcattg aaaatattta tcataatttt taatagaaaa
     2161 cactattaaa gagaaaaaag agaggtaaat caaatgaaaa aat
//
LOCUS       DACECJ010000161         2103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.167, whole genome shotgun sequence.
ACCESSION   DACECJ010000161 DACECJ010000000
VERSION     DACECJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.167"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWO09_002801"
     CDS             169..1539
                     /locus_tag="IWO09_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAQ0265351.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWO09_002802"
     CDS             1560..1844
                     /locus_tag="IWO09_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265352.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cat
//
LOCUS       DACECJ010000162         2065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.169, whole genome shotgun sequence.
ACCESSION   DACECJ010000162 DACECJ010000000
VERSION     DACECJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.169"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..492
                     /locus_tag="IWO09_002803"
                     /pseudo
     CDS             1..492
                     /locus_tag="IWO09_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="IWO09_002804"
                     /pseudo
     CDS             712..1008
                     /locus_tag="IWO09_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1171..1617
                     /locus_tag="IWO09_002805"
     CDS             1171..1617
                     /locus_tag="IWO09_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265353.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="IWO09_002806"
     CDS             1619..1756
                     /locus_tag="IWO09_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0265354.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="IWO09_002807"
     CDS             1794..>2065
                     /locus_tag="IWO09_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265355.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DACECJ010000163         2058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.170, whole genome shotgun sequence.
ACCESSION   DACECJ010000163 DACECJ010000000
VERSION     DACECJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.170"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..337)
                     /locus_tag="IWO09_002808"
     CDS             complement(<1..337)
                     /locus_tag="IWO09_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0265356.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="IWO09_002809"
     CDS             complement(404..559)
                     /locus_tag="IWO09_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0265357.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="IWO09_002810"
     CDS             1176..1397
                     /locus_tag="IWO09_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265358.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="IWO09_002811"
     CDS             1398..1655
                     /locus_tag="IWO09_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265359.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="IWO09_002812"
     CDS             complement(1791..>2058)
                     /locus_tag="IWO09_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0265360.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DACECJ010000164         2033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.171, whole genome shotgun sequence.
ACCESSION   DACECJ010000164 DACECJ010000000
VERSION     DACECJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.171"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IWO09_002813"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IWO09_002813"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0265361.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IWO09_002814"
     CDS             complement(1431..1823)
                     /locus_tag="IWO09_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0265362.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          662 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tat
//
LOCUS       DACECJ010000165         2002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.172, whole genome shotgun sequence.
ACCESSION   DACECJ010000165 DACECJ010000000
VERSION     DACECJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.172"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWO09_002815"
     CDS             <1..378
                     /locus_tag="IWO09_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0265363.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWO09_002816"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWO09_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0265364.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2002
                     /locus_tag="IWO09_002817"
     CDS             1859..>2002
                     /locus_tag="IWO09_002817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0265365.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tt
//
LOCUS       DACECJ010000166         1982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.173, whole genome shotgun sequence.
ACCESSION   DACECJ010000166 DACECJ010000000
VERSION     DACECJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.173"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1982)
                     /locus_tag="IWO09_002818"
     rRNA            complement(<1..>1982)
                     /locus_tag="IWO09_002818"
                     /product="23S ribosomal RNA"
BASE COUNT          424 a          601 c          417 g          540 t
ORIGIN      
        1 taagtcctcg atcgattagt atcagtccgc tccatacatc actgtacttc cactcctgac
       61 ctatctacct gatcatctct cagggatctt actttcttaa agaaatggga aatctcatct
      121 tgaggtgggc ttcacactta gatgctttca gcgtttatcc cttccctaca tagctaccca
      181 gcaatgccct tggcagaaca actggtacac cagcggtaag tccatcccgg tcctctcgta
      241 ctaaggacag ctcctctcaa atttccaacg cccgcgacgg atagggaccg aactgtctca
      301 cgacgttctg aacccagctc gcgtgccgct ttaatgggcg aacagcccaa cccttgggac
      361 cgactacagc cccaggatgc gacgagccga catcgaggtg ccaaacctcc ccgtcgatgt
      421 ggactcttgg gggagataag cctgttatcc ccagggtagc ttttatccgt tgagcgatgg
      481 cccttccatg cggaaccacc ggatcactaa gcccgacttt cgtccctgct cgacttgttg
      541 gtctcgcagt caagctccct tctgccttta cactcttcga atgatttcca accattctga
      601 gggaaccttt gggcgcctcc gttacctttt aggaggcgac cgccccagtc aaactgccca
      661 tctgacactg tctcccacca cgattagtgg tgcgggttag agtggccata acgcaggggt
      721 agtatcccac cagcgcctcc atcgaaacta gcgttccgat ttctacggct cctacctatc
      781 ctgtacatgc ggtacagaca ctcaatatca aactacagta aagctccatg gggtctttcc
      841 gtcctgtcgc gggtaacctg catcttcaca ggtactaaaa tttcaccgag tctctcgttg
      901 agacagtgcc caaatcgtta cgcctttcgt gcgggtcgga acttacccga caaggaattt
      961 cgctacctta ggaccgttat agttacggcc gccgtttact ggggcttcaa ttcgtacctt
     1021 cgcttgcgct aagcactcct cttaaccttc cagcaccggg caggcgtcag cccctatact
     1081 tcatcttacg attttgcaga gacctgtgtt tttgataaac agtcgcttgg gcctattcac
     1141 tgcggctgat cgtatgatca gcaccccttc tcccgaagtt acggggtcat tttgccgagt
     1201 tccttaacga gagttctctc gctcacctta ggattctctc ctcgactacc tgtgtcggtt
     1261 tgcggtacgg gccgctgttt tctcactaga agcttttctc ggcagtgtga catcaggaac
     1321 ttcggtacta ttatttccct ccccatcaca gcttgtcctt aagaaaagaa gcatttgact
     1381 cctcttcaga cttactgctt ggacagacat ttccgatcgt ctgcattcct tagcctcctg
     1441 cgtccctcca ttgctcaaac aaaaacaacg ggtacaggaa tatcaacctg ttatccatcg
     1501 cctacgcctg tcggcctcgg cttaggtccc gactaaccct gggcggacga gccttcccca
     1561 ggaaacctta gtcattcggt ggacaggatt ctcacctgtc tttcgctact cataccggca
     1621 ttctcacttc taagcgctcc agcagtcctc acgatctgcc ttcaacgccc ttagaacgct
     1681 ctcctaccaa tacacctaaa ggtgtactcc acagcttcgg taatatgttt agccccggta
     1741 cattttcggc gcagggtcac tcgactagtg agctattacg cactctttaa atggtggctg
     1801 cttctaagcc aacatcctag ttgtctgtgc aaccccacat ccttttccac ttaacatata
     1861 ttttgggacc ttagctggtg gtctgggctg tttccctttc gactatggat cttatcactc
     1921 acagtctgac tcccggatat gaatgaatgg cattcggagt ttatctgaat tcggtaaccc
     1981 ga
//
LOCUS       DACECJ010000167         1962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.174, whole genome shotgun sequence.
ACCESSION   DACECJ010000167 DACECJ010000000
VERSION     DACECJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.174"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..272)
                     /locus_tag="IWO09_002819"
     CDS             complement(<1..272)
                     /locus_tag="IWO09_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265366.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="IWO09_002820"
     CDS             complement(309..446)
                     /locus_tag="IWO09_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0265367.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="IWO09_002821"
     CDS             complement(448..894)
                     /locus_tag="IWO09_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265368.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1342..1962)
                     /locus_tag="IWO09_002822"
                     /pseudo
     CDS             complement(1342..1962)
                     /locus_tag="IWO09_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DACECJ010000168         1833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.175, whole genome shotgun sequence.
ACCESSION   DACECJ010000168 DACECJ010000000
VERSION     DACECJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.175"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IWO09_002823"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IWO09_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IWO09_002824"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IWO09_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0265369.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          551 a          345 c          357 g          580 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatatttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aat
//
LOCUS       DACECJ010000169         1833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.176, whole genome shotgun sequence.
ACCESSION   DACECJ010000169 DACECJ010000000
VERSION     DACECJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.176"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..969
                     /locus_tag="IWO09_002825"
     CDS             <1..969
                     /locus_tag="IWO09_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0265370.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="IWO09_002826"
     tRNA            1159..1232
                     /locus_tag="IWO09_002826"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          605 a          317 c          346 g          565 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DACECJ010000170         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.177, whole genome shotgun sequence.
ACCESSION   DACECJ010000170 DACECJ010000000
VERSION     DACECJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.177"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWO09_002827"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWO09_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0265371.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACECJ010000171         1647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.178, whole genome shotgun sequence.
ACCESSION   DACECJ010000171 DACECJ010000000
VERSION     DACECJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.178"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            137..475
                     /locus_tag="IWO09_002828"
     CDS             137..475
                     /locus_tag="IWO09_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265372.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="IWO09_002829"
     CDS             complement(680..1255)
                     /locus_tag="IWO09_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0265373.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          511 a          342 c          277 g          517 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggt
//
LOCUS       DACECJ010000172         1634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.179, whole genome shotgun sequence.
ACCESSION   DACECJ010000172 DACECJ010000000
VERSION     DACECJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.179"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..868
                     /locus_tag="IWO09_002830"
     CDS             101..868
                     /locus_tag="IWO09_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0265374.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="IWO09_002831"
     CDS             1060..1458
                     /locus_tag="IWO09_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ0265375.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          592 a          212 c          315 g          515 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgt
//
LOCUS       DACECJ010000173         1543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.182, whole genome shotgun sequence.
ACCESSION   DACECJ010000173 DACECJ010000000
VERSION     DACECJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.182"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWO09_002832"
     CDS             191..445
                     /locus_tag="IWO09_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0265376.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWO09_002833"
     CDS             446..763
                     /locus_tag="IWO09_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0265377.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1397..>1543)
                     /locus_tag="IWO09_002834"
     CDS             complement(1397..>1543)
                     /locus_tag="IWO09_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0265378.1"
                     /translation="EKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRY
                     SLGY"
BASE COUNT          546 a          231 c          290 g          476 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
     1501 ctttaacaga acgtgagttg aaattttgac aacctaattt ttc
//
LOCUS       DACECJ010000174         1520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.183, whole genome shotgun sequence.
ACCESSION   DACECJ010000174 DACECJ010000000
VERSION     DACECJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.183"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          572 a          172 c          310 g          466 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc
//
LOCUS       DACECJ010000175         1486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.185, whole genome shotgun sequence.
ACCESSION   DACECJ010000175 DACECJ010000000
VERSION     DACECJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.185"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..210
                     /locus_tag="IWO09_002835"
     CDS             <1..210
                     /locus_tag="IWO09_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAQ0265379.1"
                     /translation="ARIMPSSEFLLGHSLQAFDEQGRLIDEEQVAKLRELFKDFLLFV
                     TITSQLQHAHQANKREAESFSWETI"
     gene            230..1450
                     /locus_tag="IWO09_002836"
     CDS             230..1450
                     /locus_tag="IWO09_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0265380.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
BASE COUNT          514 a          268 c          300 g          404 t
ORIGIN      
        1 gcacgaatta tgccaagctc agaattttta ttgggtcatt cactccaagc ctttgatgag
       61 caaggacgac tcattgatga agaacaagtc gcaaaattac gtgaactatt caaagatttc
      121 ttactctttg tcacaatcac tagtcaattg caacatgcgc accaagcaaa taaacgtgaa
      181 gccgaaagct tttcatggga aacgatttaa tcgaggggag caaacaaaga tgaaattaat
      241 aggtatcgtc ggatcagcag ctgaactttc atataaccgt aaattattaa catttatcca
      301 aaaacaatat catgaactat ttaatttaga aatcattgaa atcaaagatt tacctctgtt
      361 caatcaagat gatgatcaaa caaatagtcc tgctattcaa cagctaaatc gcaaaatctt
      421 aatggctgat ggggtaatta ttgctacgcc agaacacaat catacggttc cagcaggatt
      481 gaaaagtgca cttgaatggc tatcatttaa aatccatcca cttgaaaata aacctgtaat
      541 ggttgtgggt gcttcttact acgatcaagg aacttcacgc gcgcaattac acttgcgcca
      601 aattttggac gcaccaggcg tgaatgcatt agtgatgccc ggaaatgaat ttttattggg
      661 aaaagcaaaa gaagcatttg accaagaaga tcgcttgatt gatccacgaa ctgttgactt
      721 tttaggcagt acattagcaa gttttgtaaa attcattaaa gtcgtgtcta ccttagcagg
      781 gcctaaaaag cttccaccag aagatttgca tgcgacagga acaattgcta cgacaatacc
      841 cgatgttgat atgtcagatg atcattggtt ggaaaaagct tctgaagcag tcggtgccgt
      901 ttctggaaat acttacgtca aattaaatcg tgggttattg acagtggatc aaatcaatta
      961 ctttttagat tcgatgccga tggaattaac ttttgcagat aataataacc aatttttata
     1021 ttataatttg cgccaagaag cgaaagagat gttagctgct cgtgtgcccg gtcaggtagg
     1081 gaatccatta gccaactgtc acccagaaaa atcgtataaa aatgtagagt gggtaattca
     1141 acaactgcga agcggagcaa cagatgcggt acgtgttcat gtaccaaaac aagtgccaga
     1201 aaaatatatt gtgcataatt accaagcaat gcatgatgaa aatggagagt atgctggaat
     1261 caatgaatac attcttgatt tcaaaccaat cattgagtgg tatttacaac aaacgggtca
     1321 acaacttgtt ggaaatgaag atgcggtttc aagtgcatct gttcatggtg gaaaaacaga
     1381 tgcagtaacc agcgccagca cacacgatga aactgcagta gatgcagttt cgagtgcttc
     1441 tgtaaaataa aaaggttctg ttgcaaagtt ttaaataaag aataaa
//
LOCUS       DACECJ010000176         1486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.186, whole genome shotgun sequence.
ACCESSION   DACECJ010000176 DACECJ010000000
VERSION     DACECJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.186"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            288..695
                     /locus_tag="IWO09_002837"
     CDS             288..695
                     /locus_tag="IWO09_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265381.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="IWO09_002838"
     CDS             709..1110
                     /locus_tag="IWO09_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265382.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="IWO09_002839"
     CDS             1112..1483
                     /locus_tag="IWO09_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265383.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          474 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatga
//
LOCUS       DACECJ010000177         1462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.187, whole genome shotgun sequence.
ACCESSION   DACECJ010000177 DACECJ010000000
VERSION     DACECJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.187"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(13..1152)
                     /locus_tag="IWO09_002840"
     CDS             complement(13..1152)
                     /locus_tag="IWO09_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0265384.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          384 a          315 c          263 g          500 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgtccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tt
//
LOCUS       DACECJ010000178         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.188, whole genome shotgun sequence.
ACCESSION   DACECJ010000178 DACECJ010000000
VERSION     DACECJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.188"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWO09_002841"
     CDS             105..1406
                     /locus_tag="IWO09_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0265385.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DACECJ010000179         1420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.190, whole genome shotgun sequence.
ACCESSION   DACECJ010000179 DACECJ010000000
VERSION     DACECJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.190"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWO09_002842"
     CDS             complement(150..1337)
                     /locus_tag="IWO09_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0265386.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          309 c          249 g          449 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact
//
LOCUS       DACECJ010000180         1339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.192, whole genome shotgun sequence.
ACCESSION   DACECJ010000180 DACECJ010000000
VERSION     DACECJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.192"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1309
                     /locus_tag="IWO09_002843"
     CDS             <1..1309
                     /locus_tag="IWO09_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ0265387.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          569 a          102 c          226 g          442 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaa
//
LOCUS       DACECJ010000181         1339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.193, whole genome shotgun sequence.
ACCESSION   DACECJ010000181 DACECJ010000000
VERSION     DACECJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.193"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1338
                     /locus_tag="IWO09_002844"
     CDS             <1..1338
                     /locus_tag="IWO09_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0265388.1"
                     /translation="SVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEE
                     QFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVP
                     KPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQN
                     ALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDE
                     SRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDI
                     PAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLD
                     AAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGII
                     LSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          446 a          243 c          286 g          364 t
ORIGIN      
        1 tctgtaagtg ttccagaagc agctgccacc attattccag taaaaggaca tcaacgagtt
       61 gtaggaacga aagcagacaa aatcaaacac ttacctataa cagagaaaga acatctcctt
      121 cctttggaag aacaattttg tgaacattgc ggttcacaga tgaaagatat cggacggaca
      181 aaagtacgag aagaaattcg tttccatcag gctatgttgg attgtctcac ccattatcaa
      241 catacctatt gttgtaaaag ctgcgaaaaa gaaggacttt cttcttttaa aaaagcgatc
      301 gttcctaaac ctttgatttc taatagctta ggttccaata gtttagtggc ggaaacgatc
      361 cggatgaaat ttggtcagaa agttcctgcc tatcggcaag agaactattg gaaacaaaca
      421 catgggttgg atatttcgag agataatatc accaattggc atattaaagc agtgcaaaac
      481 gcgttagacc ctttaggaga acgattaaga gtctatctta atcaagaaga gattctacac
      541 ggggatgaaa caagttatcg tgtgattgaa agtgccaaaa cagataccta ttactggcag
      601 ttttgtacag gaaaagacag tcagcatccc atcgtttatt atcaccacga cgaaagtcgt
      661 gctggagatg ttccgaaaac ttttctcaag gagtttacag ggtatttaca ctgtgacggc
      721 tatagcgggt ataatgccgt ggaaagtgta cgacttgtct attgtttcgc gcatgttcgg
      781 cggaaatttt tcgaagcaat tccaaaagga aagaaaaaca cggatattcc cgcagctcaa
      841 gcagtcaaac aattggataa atggtttgtc ctagaaaaga aatggaaaga cttttctcct
      901 gagaagcgat taagctgtcg gcaacaagaa ctgcgtccat tatttattgc tttttatgag
      961 tggatggcaa cgatagatcc tgttgcaaaa tccaagttgg atgcagccgt tcaatatgcg
     1021 tgtaaactac gaagtggttt tgaacctatt tttgaagatg gtcgtttaga actcacaaat
     1081 aatcgtgcgg agcgaaatat caaagagctg gtgataggac ggaaaaattg gttgcattcc
     1141 acaagtcttg aaggagcacg aacttcagga atcattctga gtgtatataa aacagcagaa
     1201 cttaacggat tgaatcctgt aaagtatcta gagtttctat ttgataagat accaaatctt
     1261 cccgtacttt cagcagaaac tttagatcaa ctgttgcctt ggaacaagga tgtacaacaa
     1321 catttttctc gtaactaaa
//
LOCUS       DACECJ010000182         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.194, whole genome shotgun sequence.
ACCESSION   DACECJ010000182 DACECJ010000000
VERSION     DACECJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.194"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            42..>1292
                     /locus_tag="IWO09_002845"
     CDS             42..>1292
                     /locus_tag="IWO09_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0265389.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          400 a          256 c          251 g          385 t
ORIGIN      
        1 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
       61 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      121 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      181 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      241 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      301 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      361 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      421 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      481 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      541 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      601 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      661 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      721 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      781 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      841 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      901 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
      961 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1021 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1081 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1141 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1201 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
     1261 cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACECJ010000183         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.195, whole genome shotgun sequence.
ACCESSION   DACECJ010000183 DACECJ010000000
VERSION     DACECJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.195"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(34..957)
                     /locus_tag="IWO09_002846"
     CDS             complement(34..957)
                     /locus_tag="IWO09_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265390.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT          421 a          228 c          127 g          516 t
ORIGIN      
        1 tttttctttt acgataaatc aatccttatt aaattattta ggaaaatatt gttcaattat
       61 ttctttatat attttctcat agtatgtttc tgtccatcct aatgttccta taaaatcaag
      121 tcctctacat tttaaaggtt cacttaaact attaatatag ttaataatgt tgtctatcct
      181 tttctcttta tcttcacctt caatctgggg agcatatagt ccaatttcct cccaataata
      241 tgaatctctt tcaattccca tgatggtagc tttggctaat ttcaaatcaa ataacgattt
      301 tagaattctt agtgtctcaa taaagtcttc tctcttcaac tctttaatcc attgtgaatt
      361 ttctggatta tctaaaattt caagttcatc ttcattataa gtatatttca aatttgactt
      421 gcttaactca tagataaatg aagtttcttg actcataatt cttttaagta cttgactata
      481 agtttcgttt gactttgccc atttcgctaa ttctaccact gtattcacta ataaatcgaa
      541 ataatgatgt tctcgttcaa tagtctcagc attttgaaaa tgaaaatcac ctttataaac
      601 tccattcttt tccactgttt tattaatatt ttttaaaaac cagaatgagt ttgcaaattt
      661 agtcgtactt tttcgattaa ctaagttaat aactcttcca tgtaataata agacaaaaat
      721 acataaattt ttagcttcct tattattaga tactaattga tcaatcatag ggagccattt
      781 tttgattttc actaattgat tgctcataaa tctttcactg gacttttgtt caatcttaaa
      841 ttgcaaaact tctaactcat aattcccatc ttctgattta tttttgggaa caactatcaa
      901 acttttataa agtttatcca catcaaaatt tatctttttt gcataatttg tagccattaa
      961 tacatctcct tattcataat aaatttcata tgacgctcct ctcaaaattt accggtagac
     1021 tagaaaaaag aataaaagat acgtcaattc acactttatt cttaaaaaaa aactggtctc
     1081 tctaccggta atatcgattg agtccgcctt tatcgacttt atcctttttt tatattaaat
     1141 tttttcaccg gtagacaggg agagaaagaa taaactaaaa agcccttaat tcttaatgtt
     1201 ttcacctcct taccgttcct accgctaaaa tttaatataa ctaaattcta taacattaga
     1261 agaaatttta aatacctttg ataaaaaata tt
//
LOCUS       DACECJ010000184         1271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.196, whole genome shotgun sequence.
ACCESSION   DACECJ010000184 DACECJ010000000
VERSION     DACECJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.196"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            265..957
                     /locus_tag="IWO09_002847"
     CDS             265..957
                     /locus_tag="IWO09_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0265391.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          554 a          144 c          183 g          390 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatgataac
     1201 atatggaaac tagttagtaa taataacagg tgattaaact tttgaacgac ttgtaattct
     1261 atattttttc t
//
LOCUS       DACECJ010000185         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.197, whole genome shotgun sequence.
ACCESSION   DACECJ010000185 DACECJ010000000
VERSION     DACECJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.197"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            20..1099
                     /locus_tag="IWO09_002848"
     CDS             20..1099
                     /locus_tag="IWO09_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0265392.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          376 a          301 c          310 g          278 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaac ccatgataac acagtttcac gcaagggaaa
       61 acatctttcc tactcagaac gttgccaaat cgctatcctg aaaaaggaag gctattccaa
      121 ccgtcaggtt gccaaagtgc tgggacgtgt tccgcaaacc ataaacaatg agattgatcg
      181 cggaaccatc acccagttaa aacgccaaac gcaaaatgga aaagtgtatg acacctacta
      241 ttctgtttat gacgctgatg caggacaggc cttctatgaa aagcaacgac tgaactgtgg
      301 cagacgcccc aaatgggcga acacaaatgc cttcattgat tgggcagatg acaaaatgtt
      361 aaatgaaaaa tggtcgccgg acgtcgtggt cggatttgcc agaaaacacg agctgttcga
      421 ttgtgccatc attcctggca caaccgctct ttatcaatgg attgaccgcg gaatcatgcg
      481 aacaaagaac atggacttgc ttgaaaaact gtcccgtaaa atcaaagcgg tccgcccagg
      541 acaccgtccc aacaagcgtg ttctcgggac gtcaattgaa gaacgcccta aagaagttga
      601 aacacgggaa acgttcggtc attgggaaat cgacacggta gtgggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgc tggctgaacg gcagacacgc tttgaagtca tcctaaagtt
      721 gaacgggaag gacaccgaat cggtggacca agcggttcaa cagctaagag aacgggctgg
      781 cgctaacttc gatcaattat ttaagaccgt tacatccgac aatggctcgg agttttctgg
      841 gctacacgat gctctgcaag atgtagctga cgtctatttc agccacccct atgcttcttg
      901 ggagcgtggg acgagcgaaa accagcacaa attaattcgc cgtttcgtcc ccaagggaag
      961 cccgattagt gaggtgagcg agaaccagat tttgcggatc cagcgttgga tgaacgatta
     1021 cccgcggaaa attttgggtt atgcgacacc acatgataca ttcgtccagg cattcaagca
     1081 agaacggttg gtggcttgac ccattattcc ttgataacga tcctcactcg ggacatggct
     1141 tcatgtctgg aaagctggcc tggggccaga atcgtctgaa gactatcata cctcagttca
     1201 ttatcaaggc ctcactgatt tagaataacg aggcagttac ggtatcaaac aaatgagtta
     1261 cctgt
//
LOCUS       DACECJ010000186         1258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.198, whole genome shotgun sequence.
ACCESSION   DACECJ010000186 DACECJ010000000
VERSION     DACECJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.198"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            20..1099
                     /locus_tag="IWO09_002849"
     CDS             20..1099
                     /locus_tag="IWO09_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0265393.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          332 a          342 c          355 g          229 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaaa ccatgataac acagtttcac gcaagggaaa
       61 acacctaacc tactcagaac gttgccaaat cgccatcctg aaaaaggaaa actattccaa
      121 ccgtcaggtt gcggatgtcc tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg
      181 cgggaccgtt actcagctta accgccagaa tcagaacggg aaggtctacg actattatca
      241 tgacgtctac gacgccgatg ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg
      301 cagacggccc ttatgggccg acacggacgc cttcattgaa tgggcggacg acatgatgct
      361 gttggaaaaa tggtccccgg acgtggtggt gggctttgcc agaaaacacg aattgttcga
      421 cccggccatt atccctggga ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa
      481 aacgaagaat atggacctgc tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg
      541 caaccgcccg aataaacggg tgctagggaa atccattgaa gagcgcccgg aggcggttgg
      601 tacccggaaa accttcggcc attgggaagt cgacacggtc gtaggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgt tggtggaacg gcagacacgc ttcgaggtca ttctgaagct
      721 ggccgggaag gatgccgaat cggtcaacca agcggtccga cagctgagag aacgggctgg
      781 tgcgtacttc ggccagctgt tcaagacggt tacctcggac aacggctcgg agttcgcggg
      841 actgcacgat gccttgcagg atgtggcaaa catctacttc agccacccct atgcttcctg
      901 ggagcgcggg acgagcgaaa accaacacaa gctgattcgc cgtttcatcc ccaagggcag
      961 cccaatcggc aacgtgagcg aaaaccaaat cttgcggatt cagcgttgga tgaacgatta
     1021 cccgcgaaag attttgggct atgcaacgcc gcatgacacg ttcgtccagg catttaggca
     1081 ggaacggttg acggcttgac acgtgggccc ttgacaacga gcctccctcg gcatggcttg
     1141 tgtctggaaa gctggccggg gccagccagt cgggagacta ccatgcctca gctcattgtc
     1201 aagggcccac tcgattagaa aaattagacg gttggtggta acgagcaacc gggtcacc
//
LOCUS       DACECJ010000187         1240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.199, whole genome shotgun sequence.
ACCESSION   DACECJ010000187 DACECJ010000000
VERSION     DACECJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.199"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            116..>1240
                     /locus_tag="IWO09_002850"
     CDS             116..>1240
                     /locus_tag="IWO09_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ0265394.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKT"
BASE COUNT          456 a          224 c          226 g          334 t
ORIGIN      
        1 aaaataagaa aagccaaaat tatataaggc ttttcttatt ttttattgac tatatcggtt
       61 aaccgatata taataatcat gtaaacattt ttaattaaat ttaggagttg aaaaaatgaa
      121 attatttaat ccaaaaactg ttttagctat tggaacattt gctgcctcac ttagtcttgc
      181 tggatctgtt gacgctgctt caactaacgt catgtatcgc ctttacaatc caaataataa
      241 tgaacatttt tacacctcta gcaaaaaaga acgtgaccat ctagtaaaga taaaatgggg
      301 aaattatgaa ggccccgcct gggaagcacc gactagcggt ggccatcttg tctatcgttt
      361 gtacaataaa ggtcttcgtg atcaccatta cacagctagt tgggatgaag tgaaatggtt
      421 gactaaaaat tacggctgga cttatgaagg gcctgcctgg cgttctgccg aaaaaaataa
      481 taaaccgatc taccgccttt tccttcctgg tgtaaaatct ggctcacatc actacactgc
      541 aagttggaaa gaagtacaat ggttaaccaa agactatggc tggaaatacg aaggtatcgg
      601 ttggtatggc gctgacacct ctaaacccaa agtgaataaa tcggctctag aagctcttta
      661 tacgagcgtt aagggaacga aaaaaggtga ctataccgac aatacctgga acgcattcca
      721 aaccgcttta aacaatgcta agaaagtgtt aaatgactca aaagcgactc aaaagcaagt
      781 ggatagtgcc aaaaataaat tagattctgc ttacaaagga ttaaagaaaa aaccagttcc
      841 tactgtgaat aaagctgaat tacaagcttt atacaaccga gtaaaatcaa ctgctaaagg
      901 agattttaca gaagaatctt ggaataattt tcagactgct ttatccaatg ctaaaaaagt
      961 actagacgat tctaaggttt ctcaaacaca agtaaacaat gctaaaaata gtcttgatgc
     1021 tgcatttaaa gggttgcaac acaaacctga aacgccaaaa gaatatatgg ttaccattaa
     1081 ccatattgac aatcaaacag gtcaagtgat tgctacagat caagcaaaag tgacttctgg
     1141 ttctacttac actgctaagg caaaagcatt taaatatagc gaaggtgcta aagataattt
     1201 tagctacaaa gtgattggta atgatactca aagcaaaaca
//
LOCUS       DACECJ010000188         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.200, whole genome shotgun sequence.
ACCESSION   DACECJ010000188 DACECJ010000000
VERSION     DACECJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.200"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IWO09_002851"
     CDS             414..947
                     /locus_tag="IWO09_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0265395.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACECJ010000189         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.202, whole genome shotgun sequence.
ACCESSION   DACECJ010000189 DACECJ010000000
VERSION     DACECJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.202"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(305..661)
                     /locus_tag="IWO09_002852"
     CDS             complement(305..661)
                     /locus_tag="IWO09_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0265396.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /locus_tag="IWO09_002853"
     CDS             complement(675..1085)
                     /locus_tag="IWO09_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0265397.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          384 a          222 c          167 g          421 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgt
//
LOCUS       DACECJ010000190         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.203, whole genome shotgun sequence.
ACCESSION   DACECJ010000190 DACECJ010000000
VERSION     DACECJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.203"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWO09_002854"
     CDS             <1..1076
                     /locus_tag="IWO09_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0265398.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACECJ010000191         1164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.204, whole genome shotgun sequence.
ACCESSION   DACECJ010000191 DACECJ010000000
VERSION     DACECJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.204"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(130..214)
                     /locus_tag="IWO09_002855"
     tRNA            complement(130..214)
                     /locus_tag="IWO09_002855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Leu,seq:caa)
     gene            complement(235..305)
                     /locus_tag="IWO09_002856"
     tRNA            complement(235..305)
                     /locus_tag="IWO09_002856"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(271..273),aa:Cys,seq:gca)
     gene            complement(351..422)
                     /locus_tag="IWO09_002857"
     tRNA            complement(351..422)
                     /locus_tag="IWO09_002857"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Gln,seq:ttg)
     gene            complement(434..506)
                     /locus_tag="IWO09_002858"
     tRNA            complement(434..506)
                     /locus_tag="IWO09_002858"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(471..473),aa:His,seq:gtg)
     gene            complement(509..582)
                     /locus_tag="IWO09_002859"
     tRNA            complement(509..582)
                     /locus_tag="IWO09_002859"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(548..550),aa:Trp,seq:cca)
     gene            complement(589..669)
                     /locus_tag="IWO09_002860"
     tRNA            complement(589..669)
                     /locus_tag="IWO09_002860"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(633..635),aa:Tyr,seq:gta)
     gene            complement(671..746)
                     /locus_tag="IWO09_002861"
     tRNA            complement(671..746)
                     /locus_tag="IWO09_002861"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(711..713),aa:Phe,seq:gaa)
     gene            complement(782..854)
                     /locus_tag="IWO09_002862"
     tRNA            complement(782..854)
                     /locus_tag="IWO09_002862"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(819..821),aa:Asp,seq:gtc)
     gene            complement(864..936)
                     /locus_tag="IWO09_002863"
     tRNA            complement(864..936)
                     /locus_tag="IWO09_002863"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(901..903),aa:Val,seq:tac)
     gene            complement(952..1023)
                     /locus_tag="IWO09_002864"
     tRNA            complement(952..1023)
                     /locus_tag="IWO09_002864"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(988..990),aa:Glu,seq:ttc)
     gene            complement(1038..1127)
                     /locus_tag="IWO09_002865"
     tRNA            complement(1038..1127)
                     /locus_tag="IWO09_002865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1091..1093),aa:Ser,seq:gga)
BASE COUNT          328 a          307 c          265 g          264 t
ORIGIN      
        1 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
       61 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
      121 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
      181 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
      241 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
      301 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
      361 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
      421 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
      481 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
      541 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
      601 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
      661 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
      721 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
      781 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
      841 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
      901 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
      961 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
     1021 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
     1081 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
     1141 ctcaatactc tttctcaaaa tgag
//
LOCUS       DACECJ010000192         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.205, whole genome shotgun sequence.
ACCESSION   DACECJ010000192 DACECJ010000000
VERSION     DACECJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.205"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWO09_002866"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWO09_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0265399.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACECJ010000193         1124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.206, whole genome shotgun sequence.
ACCESSION   DACECJ010000193 DACECJ010000000
VERSION     DACECJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.206"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..963
                     /locus_tag="IWO09_002867"
     CDS             4..963
                     /locus_tag="IWO09_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0265400.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          386 a          214 c          221 g          303 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gatt
//
LOCUS       DACECJ010000194         1116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.207, whole genome shotgun sequence.
ACCESSION   DACECJ010000194 DACECJ010000000
VERSION     DACECJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.207"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..898)
                     /gene="vanY"
                     /locus_tag="IWO09_002868"
     CDS             complement(<1..898)
                     /gene="vanY"
                     /locus_tag="IWO09_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0265401.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          160 g          400 t
ORIGIN      
        1 tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg tttcctgata
       61 tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca ggataataaa
      121 agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct tttaggtaat
      181 ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt ggtaaaccaa
      241 catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg tcctctggat
      301 aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct tcaggggctc
      361 gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta tgctcactat
      421 aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt tgctcatcaa
      481 agtctcgata gccactatta ataataaaat gactaacgcc accctttaca gcatcattga
      541 ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta tcaagcaacc
      601 cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac ttcacacttt
      661 cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa acctgttctt
      721 ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa tgttctttgg
      781 gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct tcattaacgt
      841 agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac ttcttcattt
      901 tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat cttcccctta
      961 aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag tcaattattt
     1021 cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt gatacccatg
     1081 ctgaatttaa cgaggctgaa ctgcctgttg acaatg
//
LOCUS       DACECJ010000195         1087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.208, whole genome shotgun sequence.
ACCESSION   DACECJ010000195 DACECJ010000000
VERSION     DACECJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.208"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            82..>1087
                     /locus_tag="IWO09_002869"
     CDS             82..>1087
                     /locus_tag="IWO09_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0265402.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     P"
BASE COUNT          366 a          208 c          212 g          301 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccct
//
LOCUS       DACECJ010000196         1064 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.209, whole genome shotgun sequence.
ACCESSION   DACECJ010000196 DACECJ010000000
VERSION     DACECJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.209"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            81..782
                     /locus_tag="IWO09_002870"
                     /pseudo
     CDS             81..782
                     /locus_tag="IWO09_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          351 a          176 c          181 g          356 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacaat tattccgtta tatttatgga tgcgacgcat atccctgtga
      121 agcgacagac tgtttcgaag gaagcagtgt atattacgat tggtattcgt ttggatggaa
      181 ccaaagaagt tcttgggttt actattgccc caactgagtc tgcttatatt tggaaagaag
      241 ttcttcaaga tcttagaaaa cgtgggttag aagaagtttt attagtagtg acagacggat
      301 taagctgtat tgaagaaagt atccatagtg tgtatccgaa tgcccaattt caacaatgtt
      361 gtgtgcatgt atctagaaat atcgctcata aagttcgtgt tcgaggtcga aaagaaattt
      421 gtgaggattt caaattggtt taccaagcga attcaaaaga agaggcattg gatcacatcg
      481 actttatgac taggaaatgg aaaaagcagt atccaagagt cgtcaattta ctcttgaatc
      541 ctgccctatt aaccttttat aatttccctc acgccatcag acgaacaatt tattcgacga
      601 acctgattga aggctttaat aagcagctaa aacgatatac tcgaagaaaa gaacaattcc
      661 ctaatgaaga atctctagag agattcttca tttctcaatt taatcaatat aaccaaaaat
      721 ttttaggtag aattcacaag ggatttaaag aaattcagga tacattagag tcgatgattt
      781 aactgtaatt aacagaatgg attttccatt tacatataat tcttgacgct accataatct
      841 aaataaccat catattcatc tactacttat ataattgagt tttattaaac tttttaagtt
      901 tctattctat caaatattta aacaatctct gcatccgaaa agactacctc gtatagtttt
      961 actaacaata tttttattag cgtgttcaaa tcctgatttc ttgtatcaat tattttctgt
     1021 tcactttcaa taagaaaaaa gtctttactt ccatggataa taat
//
LOCUS       DACECJ010000197         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.210, whole genome shotgun sequence.
ACCESSION   DACECJ010000197 DACECJ010000000
VERSION     DACECJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.210"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO09_002871"
     CDS             complement(36..992)
                     /locus_tag="IWO09_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265403.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACECJ010000198         1056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.211, whole genome shotgun sequence.
ACCESSION   DACECJ010000198 DACECJ010000000
VERSION     DACECJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.211"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1056)
                     /locus_tag="IWO09_002872"
     rRNA            complement(<1..>1056)
                     /locus_tag="IWO09_002872"
                     /product="16S ribosomal RNA"
BASE COUNT          226 a          329 c          237 g          264 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat caaaaccatg cggttt
//
LOCUS       DACECJ010000199         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.212, whole genome shotgun sequence.
ACCESSION   DACECJ010000199 DACECJ010000000
VERSION     DACECJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.212"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWO09_002873"
     CDS             6..980
                     /locus_tag="IWO09_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0265404.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DACECJ010000200         1034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.213, whole genome shotgun sequence.
ACCESSION   DACECJ010000200 DACECJ010000000
VERSION     DACECJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.213"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            401..1034
                     /locus_tag="IWO09_002874"
                     /pseudo
     CDS             401..1034
                     /locus_tag="IWO09_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          357 a          183 c          186 g          308 t
ORIGIN      
        1 aataaaatat tttttatatt acaaaagtag tgtttcaaag ttagtagaga aaatgtagtt
       61 atatatttaa atttttacca gaaagaaatg aacaccttca attatctaaa tctcgaaaat
      121 ggttagatgt aatcatcagg aaaaacaatc ttaaacgaat tactattcat ggtcttagac
      181 acacacatgc tagcttgctt ttagcaagct agtgcaaata ttaaagatgt gcaagagcgt
      241 ttaggccact cttctattca aatcattatg gatctatata ttcatattac agacaaaaga
      301 aaagaagaaa ccgtagcgca attcgcaaaa tataggctct ataatttatg ggactgatga
      361 atcaaaattg attcttcagt cccattgtta ttttaggtat atgattcgta cattgaaaga
      421 gttgaaaaac tatcttccga agccgtcaaa aaggaccctg ccaaaagtat tgatggtgga
      481 tgaatttcgt tcgcacgctt caattgtgga taaaatgagc tttatttgtg cggatggcga
      541 gactggaaaa ctcattgata ttttgcctac acgaaaattg tctcgattaa cacactattt
      601 tagtcgctgt gcaaatcctg aagcggtgga atatttagtg acagatatga acgccgccta
      661 cttccaactg acaaaacgtg tcttaccaaa tgcgaaagtc gtcattgatt gttttcatat
      721 catcaaacat ttgaatcagg cgttcaatga ttttcgtgta caagaaatga aaagattaca
      781 ccagttaggg caacgacggg aagccgagaa agtgaaaaag aactggcgct ttcttctaaa
      841 aaaccgtcaa aaaatcaatc actatgatta caaaacatgg cgcagttttc gtgcacctaa
      901 atacccgcta ctcactgaag caatgatgat tgatcgattg ctggcatttt caacgccttt
      961 aaaggacgcc tatacttttt tccatgagct gaccgaagcc tttagagaca aggaatcaga
     1021 cttatttttt acct
//
LOCUS       DACECJ010000201         1021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.214, whole genome shotgun sequence.
ACCESSION   DACECJ010000201 DACECJ010000000
VERSION     DACECJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.214"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..555
                     /locus_tag="IWO09_002875"
     CDS             <1..555
                     /locus_tag="IWO09_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0265405.1"
                     /translation="DLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRN
                     ISHKVRVNNRKEVCDDFKMVYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLL
                     TFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFL
                     GRSHKGFQQAEGELEQMLSQPMEN"
BASE COUNT          335 a          181 c          190 g          315 t
ORIGIN      
        1 gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg
       61 atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac
      121 gtttttcgta atatcagtca caaagtgcgt gtcaataatc gtaaggaagt ctgtgatgat
      181 tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tagaagcacg tggtgctttt
      241 gcgaaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac
      301 ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg
      361 attgaatcct ttaataagca aatcaagaaa tacagccacc gcaaggaaca gttccaaaac
      421 gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta
      481 ggtcgcagtc ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctgagtcaa
      541 ccgatggaga attagaaaca atctacagta gggaagaacc atttacacaa aattattgac
      601 gctcccaagc taaaactcct attaatcgat ctactaaaaa taaataaaaa aattcttttc
      661 aatttttatt tatggtatga cataaataaa gtaaagttat tcttctaggt taatcttatt
      721 aatactcttt ctcactagtg ttgcatgcaa cactagtgag aaagagtatt aaatatactg
      781 caaggcaaga catttggtaa ttgaagaata acaagcaaaa aaatagcagt caggtctctt
      841 gacaaagtat agttgctatt ctttttttag gccctacttt aatgaaattt ttgtttggag
      901 ggaacgactt cattcctttg attatttcag ttgaatttaa tagagaagcc tggcgtctat
      961 taagaaatac ttttcttgat gagcgtcaat ttttttaacg gaaatctatc ctatactaaa
     1021 t
//
LOCUS       DACECJ010000202         1011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.215, whole genome shotgun sequence.
ACCESSION   DACECJ010000202 DACECJ010000000
VERSION     DACECJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.215"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1011
                     /locus_tag="IWO09_002876"
     CDS             <1..>1011
                     /locus_tag="IWO09_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0265406.1"
                     /translation="TTTEFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADA
                     NANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEE
                     IKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLG
                     FQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFE
                     ADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPD
                     WLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDT
                     SWS"
BASE COUNT          369 a          185 c          209 g          248 t
ORIGIN      
        1 accaccacag agtttacaaa atttgcttcg gaatttaata ctgggatttc ttccgggaac
       61 gccaaagcga atagcttgat cgttggaacg ggaagtgcca aagaattaag tgaaaccctg
      121 ttggcagatg ccaatgcaaa tgttgtaatc attacaaccc gtcaaaaact agatgcggcg
      181 ttgaaatatg caaagaaaca agaagagaaa aaaggaacca atcgcttcca aaaattaatg
      241 ggccaacata tcgtgtttgt agtagatgag tgtcatcgag cgttaagtgc tgaaaatatg
      301 gaagaaatca aaaaaatgtt tccgaaatcc acttggtttg gctttacagg tacaccgatt
      361 tttcctgaga atcggaaaca agccaaaggg caattggcac gtacaaccca tgatcagtat
      421 ggagaggtct tgcataccta taccattaag aatgcgctag aagatggctc tgtcttagga
      481 tttcaagtag agcatgaaaa caccgtggag ccgacttcac tagagaataa aatctatcgg
      541 aaattaaaag aagtagaaac atatgcagag tattcatcag aacagatcaa tcgtatgatt
      601 gatcaaatgg aacctgtcaa aaaagaaagt tatttggacc cagctgtttt tgaagcagat
      661 gaacatatcc aaaaagtcat tcataaaatg ttccgtccag ataatgccta tacaaaattt
      721 gattttcgaa atggcaggcc aacgaaatct gcaatcttaa ccacgagttc cattgacatg
      781 gccaaaaagt attatcgggc catcaaagag atgacgaaag agcctgattg gttaacaaaa
      841 gagtttccca atcagctcat tcgtgaagga cgaacgatgg aggatcctga ttttccacga
      901 atcgctatta cctattcatt agaagaaaat agccgagatt caagtgccca acaagaagaa
      961 atgcaaaaaa ttattgagga atataatcag tattatgata cttcctggtc a
//
LOCUS       DACECJ010000203          996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.216, whole genome shotgun sequence.
ACCESSION   DACECJ010000203 DACECJ010000000
VERSION     DACECJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.216"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..912)
                     /locus_tag="IWO09_002877"
     CDS             complement(25..912)
                     /locus_tag="IWO09_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0265407.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          322 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaatt
//
LOCUS       DACECJ010000204          988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.217, whole genome shotgun sequence.
ACCESSION   DACECJ010000204 DACECJ010000000
VERSION     DACECJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.217"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..>988
                     /locus_tag="IWO09_002878"
     CDS             273..>988
                     /locus_tag="IWO09_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265408.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEAAPTDSLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYI"
BASE COUNT          392 a          164 c          215 g          217 t
ORIGIN      
        1 acggcggcct ggatatgaca atcgaaaata ttccaagaat caaagaagaa atcgaacgct
       61 ttgaaaaagg tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg
      121 aaacattaga acagctgaaa gaaagatcag aaaaaggaaa agaaaatctt cttcctgaag
      181 tacaagcaat tattgacagc ggacgagtga cgcaatggaa aaaaaatcca accatttatt
      241 ttttgaaaga ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat
      301 caaaaaaata ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag
      361 aggctgcgcc aactgattct ttgaaggaga aagaaaacga tcatttctat cgagtagaat
      421 tcaacgaaaa tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag
      481 aattgatcga tcaactcaaa acgttggata accaatacgc cgcagaagca ggttattata
      541 aattctattt tgaagaagtg aagaacggag aagtagtcga tcagatccga atggatctag
      601 gggatggcgt caacagtaat caacccatct atttagagtt agcaaaattg tgcgaacaag
      661 tacctgaaaa ggaacaaaaa caaacggtca aaaatgaacc tgtttcgaaa gaacaaatgc
      721 agtcgaaaga aacaaaaaca aaacaaaagg gctatcaaaa acgaacggca gaagagatta
      781 agcaagaagt caaatccctt tcgcaacagg cactggatgc ggtgaaaaag catacccatt
      841 ctcctgaaga cgtgaaagaa cttttgaatt ttatgtctcg ttttccagaa cgatccttta
      901 gaaatcaatt attgattgag gaacaatttc caggtgcaac ggcttgttta ggtcgtgctg
      961 cgttgaaaaa agaaggaatc tatatcaa
//
LOCUS       DACECJ010000205          926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.218, whole genome shotgun sequence.
ACCESSION   DACECJ010000205 DACECJ010000000
VERSION     DACECJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.218"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(151..>926)
                     /gene="menA"
                     /locus_tag="IWO09_002879"
     CDS             complement(151..>926)
                     /gene="menA"
                     /locus_tag="IWO09_002879"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0265409.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          328 a          193 c          160 g          245 t
ORIGIN      
        1 cgactttttc acaacctcct gataaacggt gttcaaaagc ttgcatctgc ggtaataagc
       61 ccaaccaaac gaaaaatata aggagctatt tttatttggc tgttcttatt acttgatgca
      121 gggcagtttt ttcacaacct cttcatgctg ttaaatggct tgcataatga ttgttagtaa
      181 tagccccaat acataactgg cattgaataa gaccatattt ttgatggaat agctgaaact
      241 gataggctgg ggtaacgttt gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt
      301 caagaaggtg accaagatcg gccattcata gataccgctc aacaacccca ttaaaatcac
      361 gaaatagcaa gcatacatca atagctggaa taagactgtt cccgcaggac gtccgatata
      421 aaaaactagt gtatagcgat gattttcaat atcacggtca agatcccgca aattattagc
      481 caacatgata ttggcaatcg taaacaccaa gggtaaggaa gctaatacga ttgctgctat
      541 agcccaaaga tttcctacca aatcaaattg tccagatgaa aagttgactg tcagatcata
      601 gacatctgaa ccgaatgtat tgacataaac gaccatggca aaaattccaa ggcccatagt
      661 caccccactg aaaatttccc ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata
      721 gaatactcca ataaaacaac agataaatcc catcaaaagt aaaagccaac ccgtattgat
      781 cgttagaata acgccgataa caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat
      841 caatacagga gatagatttc tgcggccaat gacattttct tcatacttat atacttcaga
      901 atgagccttt tcataatcca tgtaat
//
LOCUS       DACECJ010000206          921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.219, whole genome shotgun sequence.
ACCESSION   DACECJ010000206 DACECJ010000000
VERSION     DACECJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.219"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>921
                     /locus_tag="IWO09_002880"
     CDS             <1..>921
                     /locus_tag="IWO09_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ0265410.1"
                     /translation="TVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQ
                     VQQLQLKTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETK
                     PPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVE
                     KNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFVS
                     HLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQNVAACTSE
                     ACDFKLWTTIAKKKLTATQLKEIIQNGRTSQ"
BASE COUNT          351 a          169 c          213 g          188 t
ORIGIN      
        1 acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc tatttatgaa
       61 caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga agaaacaacg
      121 atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc agtagatcta
      181 ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga agaaccgcta
      241 cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct tgaaaaagaa
      301 acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa aacagctgga
      361 aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg aatcggtaca
      421 gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat caaggtagaa
      481 aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt ggaaaaagag
      541 ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt aaagattggt
      601 gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt ctctcacttg
      661 ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt acaagaggtg
      721 aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc aaaatgtcgg
      781 gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc ctgtgatttt
      841 aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa agagatcatt
      901 caaaacggcc gaacaagcca a
//
LOCUS       DACECJ010000207          866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.220, whole genome shotgun sequence.
ACCESSION   DACECJ010000207 DACECJ010000000
VERSION     DACECJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.220"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>866
                     /locus_tag="IWO09_002881"
     rRNA            <1..>866
                     /locus_tag="IWO09_002881"
                     /product="23S ribosomal RNA"
BASE COUNT          262 a          173 c          256 g          175 t
ORIGIN      
        1 cacggtggat gccttggcac taggagccga tgaaggacgg gactaacacc gatatgcttt
       61 ggggagctgt acgtaagcta tgatccagag atttccgaat gggggaaccc aacatcttta
      121 ataggatgtt acgattgtgt gaatacatag cacattcgag gtagacgcag agaactgaaa
      181 catctaagta cctgcaggaa gagaaagaaa attcgattcc ctgagtagcg gcgagcgaaa
      241 cgggaaaagc ccaaaccagc aagcttgctt gttggggttg taggactcca atatggtagt
      301 tctttcagat agtcgaatga cttggaaaag tcagtcaaag agggtaaaaa ccccgtagac
      361 gaaatgtgga agacacctag gaggatcctg agtacggcgg aacacgagaa attccgtcgg
      421 aatccgggag gaccatctcc caaggctaaa tactccctag tgaccgatag tgaaccagta
      481 ccgtgaggga aaggtgaaaa gcaccccgga aggggagtga aatagaacct gaaaccgtgt
      541 gcctacaaca agtcaaagcc cgttaatggg tgatggcgtg ccttttgtag aatgaaccgg
      601 cgagttacga ttgcatgcga ggttaagttg aagagacgga gccgcagcga aagcgagtct
      661 gaatagggcg tttgagtatg tagtcgtaga cccgaaacca tgtgatctac ccatgtccag
      721 gttgaaggtg cggtaaaacg cactggagga ccgaacccac gtacgttgaa aagtgcgggg
      781 atgaggtgtg ggtagcggag aaattccaaa cgaacttgga gatagctggt tctctccgaa
      841 atagctttag ggctagcctc ggaatt
//
LOCUS       DACECJ010000208          841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.221, whole genome shotgun sequence.
ACCESSION   DACECJ010000208 DACECJ010000000
VERSION     DACECJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.221"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..300
                     /locus_tag="IWO09_002882"
     CDS             <1..300
                     /locus_tag="IWO09_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0265411.1"
                     /translation="TFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILR
                     RNGLPKSMDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            623..739
                     /locus_tag="IWO09_002883"
     CDS             623..739
                     /locus_tag="IWO09_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0265412.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          334 a          106 c          122 g          279 t
ORIGIN      
        1 acgtttgact gtggaaaaga attttctaac tggaaagcca ttagtaacca acatgatatt
       61 gatatatatt ttgcggaccc tggaacacct tctcaacgcc cattaaacga gaattctaac
      121 gggattctgc gtcgtaatgg actgccgaaa tcaatggatt ttagagaagt gaatcagaca
      181 tttatttcca gtgtcagcaa tcaacgtaat catattccaa gaaaatcatt gaattacaga
      241 acaccaatcg aaatattttt gagctatgta caagaagcat tttattctag cttaatttga
      301 caaatcataa ataataaaga attgaacttc tgagaaaaca aagaatcttt tttctttttt
      361 tgaattgact catggattgg cgttaaaatt ttattttgaa aaagttattt ctggtttaac
      421 tcaaaaaaag agatatgaat ctaatagtga tagaaaggat gttatttttc atgaaatggt
      481 tgttttagat ttaatacata taactaatga atgctattga atgtatttaa actaattaac
      541 aaaagaattt gaattgttaa taaaatccaa taaaatatat taataaagta gatagaaata
      601 aaaaaacaaa gactaaacat atataattgg attaaacgca atatctgcaa aatattgtaa
      661 atttaaagcc tttgtagaaa aatttactga aagacaaact gataagatta ttcagtatca
      721 tattacctat actgattaaa attttttgaa acgggatact aaataaaaaa tttatttata
      781 acagaactaa ttatttaatg cggatgcgcc aatacttggt cctgcaagag ctatttattt
      841 t
//
LOCUS       DACECJ010000209          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.222, whole genome shotgun sequence.
ACCESSION   DACECJ010000209 DACECJ010000000
VERSION     DACECJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.222"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWO09_002884"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWO09_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0265413.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACECJ010000210          805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.223, whole genome shotgun sequence.
ACCESSION   DACECJ010000210 DACECJ010000000
VERSION     DACECJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.223"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..>805
                     /locus_tag="IWO09_002885"
     CDS             6..>805
                     /locus_tag="IWO09_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAQ0265414.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          274 a          117 c          149 g          265 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gttta
//
LOCUS       DACECJ010000211          769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.224, whole genome shotgun sequence.
ACCESSION   DACECJ010000211 DACECJ010000000
VERSION     DACECJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.224"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(65..150)
                     /locus_tag="IWO09_002886"
     tRNA            complement(65..150)
                     /locus_tag="IWO09_002886"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="IWO09_002887"
     tRNA            complement(167..238)
                     /locus_tag="IWO09_002887"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="IWO09_002888"
     tRNA            complement(250..322)
                     /locus_tag="IWO09_002888"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="IWO09_002889"
     tRNA            complement(340..421)
                     /locus_tag="IWO09_002889"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="IWO09_002890"
     tRNA            complement(429..501)
                     /locus_tag="IWO09_002890"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="IWO09_002891"
     tRNA            complement(505..577)
                     /locus_tag="IWO09_002891"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
     gene            complement(591..706)
                     /gene="rrf"
                     /locus_tag="IWO09_002892"
     rRNA            complement(591..706)
                     /gene="rrf"
                     /locus_tag="IWO09_002892"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          194 a          213 c          175 g          187 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataacttaa gcgtggcgac
      601 gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc ttaacttctg
      661 tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt ggtgttatct
      721 tttattgagt aaattttgtt cactcaaaac tggatttgaa gttacataa
//
LOCUS       DACECJ010000212          738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.225, whole genome shotgun sequence.
ACCESSION   DACECJ010000212 DACECJ010000000
VERSION     DACECJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.225"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IWO09_002893"
     CDS             complement(<1..625)
                     /locus_tag="IWO09_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ0265415.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
BASE COUNT          222 a          133 c           83 g          300 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaacta
//
LOCUS       DACECJ010000213          717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.226, whole genome shotgun sequence.
ACCESSION   DACECJ010000213 DACECJ010000000
VERSION     DACECJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.226"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            58..414
                     /locus_tag="IWO09_002894"
     CDS             58..414
                     /locus_tag="IWO09_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0265416.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            404..670
                     /locus_tag="IWO09_002895"
     CDS             404..670
                     /locus_tag="IWO09_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0265417.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
BASE COUNT          288 a           98 c          146 g          185 t
ORIGIN      
        1 attcgaattt ttatgttata tactataata taaaatcaat atatgatata attgggtatg
       61 gaaaaaatat cttttgaaac atttaaaaga cctaatggtc atgatgaatt tttagaatgg
      121 ttggaaacat taccaaaaaa agatagtgcg aagcttttac gtacaataga agaaacagaa
      181 aagaatggga tgctagtagc tcaacggttg aaatgggtga aaaaattaga cactaatttg
      241 tacgaattaa gaagtaaggt aggatcaaac atccaaagag ctatctattt ccatgtggtc
      301 aatggaaggt atgtcattac ccatggattt actaaaaaaa cggagaaaac accaataagt
      361 gaacttaaac atgcaaaaga acttagagag gagtggttca caaatgatga ttaaaaaatt
      421 gattgaagaa aagcaaaaag acaaagattt tgcagaagca tatgaccgtg aaggaagaaa
      481 actcgaaact gcggttgctc tttatcatgc acgggaggaa gcaggcctaa ctcaagcgga
      541 gcttgcacaa aaagcacata caactcaatc tactattgct cgaattgaga gaggggataa
      601 cgtatctttc gataaacttg atgatatcgc tagggcatta ggcaaaaagc tggtagtaga
      661 atttgcttaa aagcgagttg aaaatatacc tgtaataggc tcctaactaa tcaatta
//
LOCUS       DACECJ010000214          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.227, whole genome shotgun sequence.
ACCESSION   DACECJ010000214 DACECJ010000000
VERSION     DACECJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.227"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..446)
                     /locus_tag="IWO09_002896"
     CDS             complement(<1..446)
                     /locus_tag="IWO09_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0265418.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DACECJ010000215          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.228, whole genome shotgun sequence.
ACCESSION   DACECJ010000215 DACECJ010000000
VERSION     DACECJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.228"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..628)
                     /locus_tag="IWO09_002897"
     CDS             complement(53..628)
                     /locus_tag="IWO09_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0265419.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DACECJ010000216          690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.229, whole genome shotgun sequence.
ACCESSION   DACECJ010000216 DACECJ010000000
VERSION     DACECJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.229"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            67..408
                     /locus_tag="IWO09_002898"
     CDS             67..408
                     /locus_tag="IWO09_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265420.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            401..>690
                     /locus_tag="IWO09_002899"
     CDS             401..>690
                     /locus_tag="IWO09_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0265421.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          260 a          100 c          137 g          193 t
ORIGIN      
        1 aaataaaaaa gtgactcagc cggcaagcat agagtcacaa aacatatcta aggagaattt
       61 tagcatatga ataaagaact ttccacttta gatcaatatt tgactgattc tgaatggggc
      121 aagtcgaata tcaaggaaac aaataatcga aaaatcagac gaaatctttt gacgaacgaa
      181 gaactatcat gtgatcaaga tgacttggga aattttgtga gtatttggga tcatgtttac
      241 cttatccatc tatcaaaaca ttcaaataaa cctgaataca tctatgtcat cgaagatggc
      301 ttgattgatg cgctagaaga gtacaacaga gataacttga ttgatatctc ttattacgga
      361 tcaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa tcaaagggac
      421 aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa
      481 ttttgattat ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg atccacaggc
      541 aacggtaact attaatgagt ataaacacta cagggttgtt tctggaactc atcaagactt
      601 tcttgttgag gaatataaac cttttcttat ggatgaaaca gggacttatg tatctgtctc
      661 agtaacggtt aaaggacata cggaaacaga
//
LOCUS       DACECJ010000217          688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.230, whole genome shotgun sequence.
ACCESSION   DACECJ010000217 DACECJ010000000
VERSION     DACECJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.230"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            124..327
                     /locus_tag="IWO09_002900"
     CDS             124..327
                     /locus_tag="IWO09_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0265422.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            327..677
                     /locus_tag="IWO09_002901"
     CDS             327..677
                     /locus_tag="IWO09_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0265423.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          287 a           69 c          115 g          217 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag
      421 atattactga tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa
      481 ttcttgtaaa taaattagtt aattatattt acatcgtggg ttttgataat aaaatacgct
      541 ttattgataa tgatgaaaaa ttattaatag aattagggga tatttctaaa aaagctggaa
      601 ttaatagtaa atacaaagca aactttacag ataaatcgca attttatagt tattcagaaa
      661 caatgcctca acgataaaat atagtata
//
LOCUS       DACECJ010000218          651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.231, whole genome shotgun sequence.
ACCESSION   DACECJ010000218 DACECJ010000000
VERSION     DACECJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.231"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWO09_002902"
     CDS             141..398
                     /locus_tag="IWO09_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265424.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          266 a           79 c           86 g          220 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa a
//
LOCUS       DACECJ010000219          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.232, whole genome shotgun sequence.
ACCESSION   DACECJ010000219 DACECJ010000000
VERSION     DACECJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.232"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..270)
                     /locus_tag="IWO09_002903"
     CDS             complement(<1..270)
                     /locus_tag="IWO09_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265425.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="IWO09_002904"
     CDS             complement(276..>635)
                     /locus_tag="IWO09_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0265426.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DACECJ010000220          595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.234, whole genome shotgun sequence.
ACCESSION   DACECJ010000220 DACECJ010000000
VERSION     DACECJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.234"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..563)
                     /locus_tag="IWO09_002905"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="IWO09_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          168 a          109 c           78 g          240 t
ORIGIN      
        1 taattgtgca attgaaagta atagattcaa tttaaattct tttttgagct gaaggattgt
       61 tttaaccttg atttcttctt gctcaaatct tgttcttgaa tcaaggtttc taactttttt
      121 aaatatgcgt tctctgctct taaacgaata acttcttctt caagagactc atctttaagt
      181 tttttaggaa tgtttagctt ggaattcata ctaatttttc tgcccctttt ttggctctca
      241 agtgaagaag caccgccttc ttcatattgc tctatccatt tacttagagt tctatttgaa
      301 ccgatattaa attttttagc agtttcttgg atagaaagac catttgtttc catatattct
      361 ataacatcaa gtttaaattt tgtagtgtag cttttgccac ctccaaccaa gccttcccaa
      421 ccatgatagt tataaatcct tacccaatgt ctaaccagtg tacgatttat ttgatattta
      481 tgtgcaagat atttgtagcc gccttcgtta tttaaatagt ctgaaactac ttttttctta
      541 aaaacaaatg tatatttccg caaaaaaagc acccctttta attagatttc tagtc
//
LOCUS       DACECJ010000221          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.235, whole genome shotgun sequence.
ACCESSION   DACECJ010000221 DACECJ010000000
VERSION     DACECJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.235"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..528
                     /locus_tag="IWO09_002906"
                     /pseudo
     CDS             1..528
                     /locus_tag="IWO09_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          218 a           91 c           96 g          123 t
ORIGIN      
        1 tcagcatttg acaaatgggt aaagcaatct aaaacgagtg gttcattcaa agaaaaagat
       61 aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca gcaattagaa
      121 atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga caagtaatcg
      181 atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt ctatcacgtc
      241 agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa gaagtagtcg
      301 ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa aaagccttat
      361 caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa aatagaggat
      421 taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc aatgaagcca
      481 aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattag
//
LOCUS       DACECJ010000222          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.236, whole genome shotgun sequence.
ACCESSION   DACECJ010000222 DACECJ010000000
VERSION     DACECJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.236"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWO09_002907"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWO09_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0265427.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACECJ010000223          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.237, whole genome shotgun sequence.
ACCESSION   DACECJ010000223 DACECJ010000000
VERSION     DACECJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.237"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(107..>514)
                     /locus_tag="IWO09_002908"
     CDS             complement(107..>514)
                     /locus_tag="IWO09_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ0265428.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DACECJ010000224          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.238, whole genome shotgun sequence.
ACCESSION   DACECJ010000224 DACECJ010000000
VERSION     DACECJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.238"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACECJ010000225          501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.239, whole genome shotgun sequence.
ACCESSION   DACECJ010000225 DACECJ010000000
VERSION     DACECJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.239"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..303)
                     /locus_tag="IWO09_002909"
     CDS             complement(<1..303)
                     /locus_tag="IWO09_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0265429.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVS"
BASE COUNT          154 a          108 c           60 g          179 t
ORIGIN      
        1 gctgacaata taatcactca aatatgacca agtatcttca acagataatt cgtctccatt
       61 atcattcata agcgtcattt taaaccattc attaccttca tcatcttcag gtagcaagcc
      121 gaatccaatt ggattatcag tcaatgtttg atcgtataaa tcatcctcgt ctaatgctaa
      181 aacatctaat cctttacaat ttccgcctct tctaaacgtc acaaatcctt tgtaatccca
      241 acgttggaaa gaaaccttaa tagtatgtgt gcccaatcgt atattcggat cataattttt
      301 catttgtcat tcctccatta cgtaaatttc attttctcta tgttttatat caattttttc
      361 actttattca ggacttgttc atattttatc gtttttcgtt tacttaaaaa tacataaaaa
      421 atttcgactc gtttatggcg tccgaaacga gaaaccctag tagacagcgg taaatgctcg
      481 attgatccaa taatcaacgt c
//
LOCUS       DACECJ010000226          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.240, whole genome shotgun sequence.
ACCESSION   DACECJ010000226 DACECJ010000000
VERSION     DACECJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.240"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>487)
                     /locus_tag="IWO09_002910"
     CDS             complement(<1..>487)
                     /locus_tag="IWO09_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ0265430.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACECJ010000227          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.241, whole genome shotgun sequence.
ACCESSION   DACECJ010000227 DACECJ010000000
VERSION     DACECJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.241"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(62..259)
                     /locus_tag="IWO09_002911"
     CDS             complement(62..259)
                     /locus_tag="IWO09_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0265431.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DACECJ010000228          452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.242, whole genome shotgun sequence.
ACCESSION   DACECJ010000228 DACECJ010000000
VERSION     DACECJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.242"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>452
                     /locus_tag="IWO09_002912"
     CDS             <1..>452
                     /locus_tag="IWO09_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265432.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DACECJ010000229          441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.243, whole genome shotgun sequence.
ACCESSION   DACECJ010000229 DACECJ010000000
VERSION     DACECJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.243"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>441
                     /locus_tag="IWO09_002913"
     CDS             <1..>441
                     /locus_tag="IWO09_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0265433.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSL"
BASE COUNT          194 a           61 c           74 g          112 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg a
//
LOCUS       DACECJ010000230          417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.244, whole genome shotgun sequence.
ACCESSION   DACECJ010000230 DACECJ010000000
VERSION     DACECJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.244"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            44..>417
                     /locus_tag="IWO09_002914"
     CDS             44..>417
                     /locus_tag="IWO09_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0265434.1"
                     /translation="MADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQ
                     EKIRNFQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGT
                     IHQVETEKKSAASPRLFSLSSL"
BASE COUNT          148 a           90 c           88 g           91 t
ORIGIN      
        1 agaagagacc tacccaaaat ttgaagaagc acaagcacgc cagatcgccg attggttgat
       61 cggcatgaac ggtagcccgc tgtatagttt actcttgcag caaaaaggca taccaggaag
      121 tttttcctta ggcagagtcc aaaccccaac gctttacatg atttatcaat tacaggaaaa
      181 gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gcccaagtaa tcgctcaaaa
      241 tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa gcgacgcaag aagcatttga
      301 agattattta aaagaaaagg gggttcaatt gggaaaacag ccaggtacga tccaccaagt
      361 tgaaaccgag aaaaaaagtg cggcaagtcc acgtcttttt tctctatcca gccttca
//
LOCUS       DACECJ010000231          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.245, whole genome shotgun sequence.
ACCESSION   DACECJ010000231 DACECJ010000000
VERSION     DACECJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.245"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IWO09_002915"
     CDS             <1..390
                     /locus_tag="IWO09_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0265435.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          115 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatt
//
LOCUS       DACECJ010000232          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.246, whole genome shotgun sequence.
ACCESSION   DACECJ010000232 DACECJ010000000
VERSION     DACECJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.246"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWO09_002916"
     CDS             complement(<1..339)
                     /locus_tag="IWO09_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0265436.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACECJ010000233          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.247, whole genome shotgun sequence.
ACCESSION   DACECJ010000233 DACECJ010000000
VERSION     DACECJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.247"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IWO09_002917"
     CDS             <1..379
                     /locus_tag="IWO09_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0265437.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DACECJ010000234          395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.248, whole genome shotgun sequence.
ACCESSION   DACECJ010000234 DACECJ010000000
VERSION     DACECJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.248"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>395)
                     /locus_tag="IWO09_002918"
     CDS             complement(<1..>395)
                     /locus_tag="IWO09_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0265438.1"
                     /translation="CQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIED
                     KMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRIL
                     PNAKVVIDRFHIIKHMNQAFNELRIREMN"
BASE COUNT          118 a           78 c           78 g          121 t
ORIGIN      
        1 cattcatttc acggatacgc aactcattaa aggcttgatt catgtgtttg ataatatgaa
       61 accgatcaat cactactttc gcatttggca gaatacgttt ggtaagctgg aagtaggcgg
      121 cgttcatgtc tgtgactaaa aattttactt cttcaggatt agcgcagcct aagaaatagc
      181 ttgttaatcg aggcaattta cgcgtaggca agacatcaat taattgtcct gtttcgccat
      241 ctgcgcaaat aaagctcatt ttatcttcta tggaagcgtg cgaacgaaat tcatcaacca
      301 tcaatactct ggggagaatc ctcttagatt gctttggtaa gtagctttta aactctttca
      361 acgtacgaat aacggtggtc aaagatacct gacaa
//
LOCUS       DACECJ010000235          379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.249, whole genome shotgun sequence.
ACCESSION   DACECJ010000235 DACECJ010000000
VERSION     DACECJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.249"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..98)
                     /locus_tag="IWO09_002919"
     tRNA            complement(25..98)
                     /locus_tag="IWO09_002919"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62..64),aa:Ile,seq:cat)
     gene            complement(112..185)
                     /locus_tag="IWO09_002920"
     tRNA            complement(112..185)
                     /locus_tag="IWO09_002920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(149..151),aa:Met,seq:cat)
     gene            complement(213..285)
                     /locus_tag="IWO09_002921"
     tRNA            complement(213..285)
                     /locus_tag="IWO09_002921"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Ala,seq:tgc)
     gene            complement(305..378)
                     /locus_tag="IWO09_002922"
     tRNA            complement(305..378)
                     /locus_tag="IWO09_002922"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(342..344),aa:Pro,seq:tgg)
BASE COUNT          103 a          109 c           90 g           77 t
ORIGIN      
        1 catatgaaac ttacaacgcc acaatggacc ttgtaggact cgaacctacg accggacggt
       61 tatgagccgt ctgctctaac caactgagct aaaggtccag gctcttaaaa atcgcggcgg
      121 aggggatcga acccccgacc tcccgggtat gaaccggacg ctctagccag ctgagctaca
      181 ccgcgaaaaa gatattcatt atttaaatga aatggagcct agcgggatcg aaccgctgac
      241 ctcctgcgtg caaagcaggc gctctcccag ctgagctaag gccccgtgtt taaatttata
      301 attatcggga agacaggatt cgaacctgcg accccttggt cccaaaccaa gtgctctacc
      361 aagctgagct acttcccgt
//
LOCUS       DACECJ010000236          375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.250, whole genome shotgun sequence.
ACCESSION   DACECJ010000236 DACECJ010000000
VERSION     DACECJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.250"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWO09_002923"
     CDS             complement(<1..132)
                     /locus_tag="IWO09_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0265439.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DACECJ010000237          373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.251, whole genome shotgun sequence.
ACCESSION   DACECJ010000237 DACECJ010000000
VERSION     DACECJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.251"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IWO09_002924"
     tRNA            complement(20..91)
                     /locus_tag="IWO09_002924"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IWO09_002925"
     tRNA            complement(113..200)
                     /locus_tag="IWO09_002925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IWO09_002926"
     tRNA            complement(225..298)
                     /locus_tag="IWO09_002926"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
BASE COUNT          111 a           97 c           86 g           79 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aac
//
LOCUS       DACECJ010000238          341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.254, whole genome shotgun sequence.
ACCESSION   DACECJ010000238 DACECJ010000000
VERSION     DACECJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.254"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>341
                     /locus_tag="IWO09_002927"
     CDS             <1..>341
                     /locus_tag="IWO09_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0265440.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DACECJ010000239          327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.255, whole genome shotgun sequence.
ACCESSION   DACECJ010000239 DACECJ010000000
VERSION     DACECJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.255"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..314
                     /locus_tag="IWO09_002928"
     CDS             <1..314
                     /locus_tag="IWO09_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0265441.1"
                     /translation="NQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTN
                     LIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESM
                     F"
BASE COUNT          132 a           48 c           45 g          102 t
ORIGIN      
        1 ataatcaaat aaggtttatg atagataaat ggaaaaagca gtatccacga gtggttaaat
       61 tgctcatgaa tcctgctata ttaacctttt ataatttccc tccgtcaatc agaagaacta
      121 tttattcaac taatttgatt gaaggtttta acaaacaatt aaagaaatat accaaaagaa
      181 aagagcaatt tcctaatgaa gaatctctgg agagatttct agtatctcaa ttcaacaact
      241 ataatcaaaa gtttctatgc cgagttcata aaggttttaa agaaatacat gatacattag
      301 aatcaatgtt ttaacttgat caacaga
//
LOCUS       DACECJ010000240          323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.256, whole genome shotgun sequence.
ACCESSION   DACECJ010000240 DACECJ010000000
VERSION     DACECJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.256"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IWO09_002929"
     CDS             complement(<1..302)
                     /locus_tag="IWO09_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0265442.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DACECJ010000241          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.257, whole genome shotgun sequence.
ACCESSION   DACECJ010000241 DACECJ010000000
VERSION     DACECJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.257"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>321)
                     /locus_tag="IWO09_002930"
     CDS             complement(<1..>321)
                     /locus_tag="IWO09_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0265443.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACECJ010000242          309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.denovo.258, whole genome shotgun sequence.
ACCESSION   DACECJ010000242 DACECJ010000000
VERSION     DACECJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.denovo.258"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..>309
                     /locus_tag="IWO09_002931"
     CDS             51..>309
                     /locus_tag="IWO09_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0265444.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DACECJ010000243        10738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.089, whole genome shotgun sequence.
ACCESSION   DACECJ010000243 DACECJ010000000
VERSION     DACECJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.089"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1868
                     /locus_tag="IWO09_002932"
     CDS             <1..1868
                     /locus_tag="IWO09_002932"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0265445.1"
                     /translation="IADEYVDMEFGTGVVKITPAHDPNDFEVGNRHDLPRVNVMDDDG
                     TMNELAGKYAGMERFAARKAIVQDLKEIGRLIKIDTMIHSVGHSERTGVVVEPRLSTQ
                     WFVKMAPLAKKAIDNQETDQAVEFFPPRFNQTFLRWMENVHDWVISRQLWWGHQIPAW
                     YHKETGEMYVGMEAPADSENWVQDPDVLDTWFSSALWPFSTMGWPDTESEDYKRYFPT
                     STLVTGYDIIFFWVSRMIFQSLEFTGERPFENVLIHGLIRDEQGRKMSKSLGNGIDPM
                     EVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDASWNFINKIWNASRFVIMNIEGMTV
                     EEIDLSGEKSVADRWILTRLNETIERVTELFDRFEFGEAGRQLYNFIWDDFCDWYIEM
                     SKEVLYGDDAVSKQTTRSILVHTLDQILRLLHPIMPFVTEEIWEHIPHQGNSLVVAEY
                     PVVRPEFDDETASKGMEVLKELIRAVRNIRSEVNTPLSKPITLLIKTNDAKIDQFLVE
                     NTSYIERFCNPEELTISSEITAPDLAMSAVLTGAEIFLPLAGLINIEEEIKRLEKELE
                     KWTSEVKRVQGKLSNERFVSNAPEEVVEAERAKEKDYLEKQTAVKERIESLRTIQ"
     gene            2178..3491
                     /locus_tag="IWO09_002933"
     CDS             2178..3491
                     /locus_tag="IWO09_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0265446.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            3481..4134
                     /locus_tag="IWO09_002934"
     CDS             3481..4134
                     /locus_tag="IWO09_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0265447.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            4189..4740
                     /gene="radC"
                     /locus_tag="IWO09_002935"
                     /pseudo
     CDS             4189..4740
                     /gene="radC"
                     /locus_tag="IWO09_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4871..5956
                     /locus_tag="IWO09_002936"
     CDS             4871..5956
                     /locus_tag="IWO09_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0265448.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            5953..7845
                     /locus_tag="IWO09_002937"
     CDS             5953..7845
                     /locus_tag="IWO09_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0265449.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            7852..8229
                     /locus_tag="IWO09_002938"
     CDS             7852..8229
                     /locus_tag="IWO09_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0265450.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            8383..9165
                     /locus_tag="IWO09_002939"
     CDS             8383..9165
                     /locus_tag="IWO09_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAQ0265451.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(9291..10022)
                     /gene="erm(A)"
                     /locus_tag="IWO09_002940"
     CDS             complement(9291..10022)
                     /gene="erm(A)"
                     /locus_tag="IWO09_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAQ0265452.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(10080..10167)
                     /locus_tag="IWO09_002941"
                     /pseudo
     CDS             complement(10080..10167)
                     /locus_tag="IWO09_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            10531..>10738
                     /locus_tag="IWO09_002942"
     CDS             10531..>10738
                     /locus_tag="IWO09_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0265453.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQM"
BASE COUNT         3703 a         1608 c         2207 g         3220 t
ORIGIN      
        1 tcattgcaga tgaatatgta gatatggaat tcgggacagg tgtagtaaaa atcactccag
       61 cacatgatcc aaacgacttt gaggtaggta atcgtcatga tcttccacgc gtaaatgtta
      121 tggatgatga tggtacgatg aatgaactag caggaaaata tgctggaatg gaacgttttg
      181 ctgctcgtaa agcaatcgtg caagatctga aagaaatcgg tcgattgatt aaaatcgata
      241 cgatgatcca cagtgtgggt cattccgagc ggacaggtgt cgttgttgaa cctcggctgt
      301 ctactcaatg gtttgtaaaa atggcgccgt tagcgaaaaa agccattgat aaccaagaaa
      361 cagatcaagc agtggaattc ttccctccaa gattcaatca aacgttttta cgttggatgg
      421 aaaatgtcca tgattgggta atctcacgac aattatggtg gggacatcaa attcctgcat
      481 ggtatcacaa agaaacaggt gaaatgtatg tagggatgga agcaccagcg gatagtgaaa
      541 actgggtaca agatcctgat gttttagaca cttggtttag ttcagctttg tggcctttct
      601 caacaatggg ctggccagat acggaaagcg aagattataa gcgatacttc cctactagca
      661 ctttagtaac cggttatgac atcattttct tctgggtaag cagaatgatt ttccaaagct
      721 tggaatttac tggcgaacgt ccatttgaga atgtcttgat tcatggattg attcgtgacg
      781 aacaagggcg gaagatgagt aaatcattag gtaatggaat cgatccgatg gaagtaatcg
      841 aaaagtatgg agcagatgct ctccgctggt tcttatcgaa tggttctgca ccaggacaag
      901 atgtccgctt cagctatgaa aaaatggatg cttcttggaa ctttatcaac aaaatctgga
      961 atgcttcacg tttcgttatc atgaatatcg aaggaatgac cgtagaagag atcgatttat
     1021 ctggagaaaa atcagtggct gaccggtgga tccttactcg tttgaatgaa acgatcgaac
     1081 gagtaactga gctatttgat cgttttgaat ttggtgaagc aggtcgacaa ctgtataact
     1141 ttatctggga tgatttttgt gactggtaca tcgaaatgag taaagaagta ctttatggag
     1201 atgatgctgt ttcaaaacaa accacccgta gcatcttagt ccatacactt gatcagatcc
     1261 tacgtttatt gcatccaatc atgccattcg ttacagaaga aatatgggaa catattcctc
     1321 atcagggaaa ttctctagta gtggcagaat atcctgttgt acgtccagag tttgatgatg
     1381 aaacagcttc taaagggatg gaagtattga aagaattgat tcgagccgtt cgaaatattc
     1441 gttcagaagt gaatacacca ttgtctaaac caattacgtt gctaattaaa acaaacgatg
     1501 ctaaaatcga tcaattttta gttgaaaata caagctatat cgaacgcttc tgtaatcccg
     1561 aagagttgac gatatcaagt gagatcactg cgccagatct agcgatgtca gctgttctga
     1621 caggtgcaga aatcttcttg ccgttagcag gattgatcaa tattgaagaa gaaatcaaac
     1681 gattagaaaa agaactggaa aagtggacaa gcgaagtaaa acgtgtccaa ggaaaacttt
     1741 ctaatgaacg ttttgtttct aacgcgccgg aagaagtagt agaggcagag cgtgcaaaag
     1801 aaaaagatta tttagagaag caaacggctg tcaaagaacg aatcgaaagt ttgcgtacta
     1861 ttcaataaga atagaaaaag agagtgtgaa atcagctagc tattactaaa agctagctga
     1921 ttgaacatcg ttacaggaaa gagtttgagc ctcactctta gcttcgattc ataaggagat
     1981 gtgacataaa ttttgtcgca tctctttttt agtctactct tattatgtag gagaaggagg
     2041 gaggtggatc atgtatcaag agtcaataaa gtagttgata aaatcgttcc tctctctgtt
     2101 tcttttctct ttgtcatact tttatttttc ttatgtgttc tatttgttat aataaaagag
     2161 aaatggggga attgttgatg acgattgaag aagcaataga atggatccat agtcgattac
     2221 catttggttc acgtcctggc cttgaccgag tcgaagcttt attagaagtg gtagggcatc
     2281 cagagagaaa agtaaaaacg atccatatcg ctggaacaaa cggaaaaggt tctactgtag
     2341 cttatttgag aagcctatta gaagaggctg ggctgacagt aggtacattt acttcacctt
     2401 atatcgaaac gttcaatgaa cggatcgcaa tcaatggcaa aagcattcca gacaatgatt
     2461 tgatccagct tgtaaaaaaa tatcagccac tgatcaaaca acttgatcaa tttgaagcag
     2521 tagctggtat tactgaattc gagacattaa cagcaatggc atttgattat tttttgatga
     2581 agaaagtgga cgtagcgatt atcgaagttg gcttaggcgg cttattggat tctaccaatg
     2641 tagctgtacc aatgctgacg ggaataacaa cgattggact tgatcatacc gaaatacttg
     2701 gagatacaat tgaagaaatc gctgcacaaa aagctggaat catcaaacaa ggcgttccag
     2761 tagtaacagg aaatattgcc ccagaagctt ttactgtcat ccaacaaaca gctaaagcaa
     2821 aaaatgcacc gctttatgta tacggggaaa attataaaat aaactatctg catcctgatg
     2881 agatgtgggg agaagtattt gaatttttta atgaaatagg aaagatccct caattaacca
     2941 ctccgctttt agggcaacat caagctgaaa atgcaggagt agccatcgaa ttgtattttg
     3001 tttactgtca attggaaaag ctgcctttcc gagaaaaaga tgttttgaag ggattgaaga
     3061 aatctcgatg gccaggaaga atggagcgtt tgagtaacga gccattagtt gttttagatg
     3121 gtgcccataa tacgcatgca atgaaccggc ttgttgagaa tttgaaaaaa gaattcaaag
     3181 gatatcaaat caacattttg ttttccgcct tacgaactaa aaatgttaga gaaatgctag
     3241 aacgattgca gcaagtacca aatgtccata tttatttgac gacctttgaa catccaaaag
     3301 ccatcgaatt aagtgaagta tcagatttag caagtgagag agtatcaatc gtttcattgt
     3361 ggcaattcgg attagcagat atattggaaa agatgacaaa tgaagacgta ttagttgtga
     3421 ccggttcact ttatttcatt tcagaagttc gccatttatt aatacaatta ggaggagatc
     3481 atgaagtata atgcagtgat ttttgacatg gatggtttgc tgttcgacac agagttagtg
     3541 tattacgaag ccacacaagt agtagcagac aagatgggtt ttccttatga taaagacctg
     3601 tatttaaaat tcttaggtgt ttcagatgaa gaagtatggg cgaattatca tatgattttc
     3661 tcggaatttg gcaaaacaag tgtacaaaga ttcatagatg attcatacca ggaaacgctt
     3721 gaacgttttt cgcaaggagc ggtacaaatg aagcccggtg tgttggaact cctctctttt
     3781 ttagaagaac atcagattcc caaagttgtc gcttccagta accaacgagc agtcattgaa
     3841 cttttattga agaagaacaa tctgtttgaa cgatttgaga cgattgtttc tgcagaaaat
     3901 gttcaacgag caaaaccaga cccagaaatt ttcttgttag ctaatcagaa gctaggtaca
     3961 gcgaagaaag aaacattgat tttagaagat tcacaaaatg gtattttagc agcttctgcg
     4021 gctgagatcc cggtagtcat ggtaccagat ctgcttccac catcccaaga attgatcaaa
     4081 aaaacagctg gggttgtttc ttctttacat gaaatccctg cattgttaaa ataaaaatcc
     4141 atctcttttc gtatcttatt ttagataaga tagaaaggag atgttttttt gggaaaacga
     4201 ttgatcaaag aagtacctac tagctcttta ccaagggaac gtatggaaat ttatggcgcg
     4261 gaagctttat cggatcagga gcttttggca atcttactgc gtaccggtca acacccttat
     4321 agtgtgatgt cgattgctgg aaatctgtta aaaacgtttg gaggactggc ctctttacga
     4381 caggctactt tgcatgagtt agaagagatt cgagggatag gacgcgtgaa agcaatcgaa
     4441 atcaaagcct tgatcgaatt aggtcgacga attcagacgg atcatgaaag agcggctcca
     4501 ccggttcgta ccagctacga attggcccag caattgattc tggaaatgaa agatcacaaa
     4561 caagaacatt tggtttgtat ttatttggac acgaagaatc aagtgatctt aaaaaaaaca
     4621 gtgttcatcg gttcgctgaa tcagtcagtc gctcatccta gagaaatttt ccattatgct
     4681 gtacgttatt gtgcagcaag gatcgtttta gcgcataatc atccttctgg taatgtttta
     4741 accatagtat aataaataat agtagttaaa gtagtttaag aggagatatt aaagggatat
     4801 tttactttaa ataaaaatta acaaggttta taatttgtag tagttaaagt atttaaagag
     4861 gtgggaacat gtgaaggttc aaaggataga agtggagaat aagccgtatc cattgtattt
     4921 attactagat aaagaatacc agctaataga accagtaatg aaatttatta aatacttaga
     4981 taatactggt aagtctccta ataccattaa agcatactgc tatcatttaa agttgctgta
     5041 cgagttcatg gaacagagag gtgttattct taatgatatt aactttgagt tgttagcaga
     5101 cttcgtaggt tggttgagat atccttcagc atcaaatgta attgatcttc agtcaaaaaa
     5161 agccataaga gaagaaacga cagtgaatac aattttaaat gtagttatga gttttcttga
     5221 ttatttaagt agattaggag aatttaaatc aattgatgta tttaaacaag caaagggaag
     5281 aaatttcaaa ggatttttac atcatgttaa taagggtaga taccaaaaga atgtcttaaa
     5341 gttaagggtt aaaaagaaac agataagaac attgagatca gaggaagtta agcaaattat
     5401 tgatgcttgt catacgaaaa gagataaatt aattttaatg cttatgtatg agggtggttt
     5461 aagaatcggt gaagtgttat cgcttaggct tgaagatatt gtcacttggg acaatcaaat
     5521 ccatttaaca cctagagatg ttaatgttaa tgaagcttat attaaattaa ggaaggaaag
     5581 aacaatacat gtgagtaaag aacttatgtc actttataca gattacttga tatatgagta
     5641 tagtgaggaa ttggagcatg attatgtttt tatttcctta aaagaaggct attttgggaa
     5701 accactaaag taccaaagtg ttcttgatct agttagaaga atagttaaaa ggactggaat
     5761 agaatttaca tcacatatgc ttcgccacac tcacgcaacg cagctaatta gggaaggatg
     5821 ggatgttgcg ttcgttcaaa agagattagg tcacgcacat gttcagacaa cgttaaatac
     5881 ctatgttcat ctttcagatc aggatatgaa aaatgagttt aataaatacc tcgagagaaa
     5941 ggagcataag aaatgaatgc ttctagtaaa aggaaaatta ttagtcagag tgagattagc
     6001 aaaaaaatag ctgtaatgaa tgaagaaatg caggggtttt gggctaataa tagttgggat
     6061 ataagaaaat gtccacatcc ttctgccata gaattaagta agaatcctgc tttaaggaat
     6121 cgttgggttc gttttgaacg tgttaaaaat ctgtggttaa gaacagaatt gaaatatttt
     6181 tatttttacc atttaaacaa tggaatatgg aatgcaaaaa ctgtctggat tagaaaagga
     6241 acagtaatta ataaaatgtt agatttctta gatttaaagt atcctagcat tacttcaatt
     6301 actgaagttc ctattgaaaa agcaatgacg gagtatagaa cttatttgac aaaacggggt
     6361 gttagaatta ccactactaa ttataagatt actgctaatc aagaaaaaac acctgtaaaa
     6421 gctaattcct actatgttac taatctaaaa caatttatgg agttttatga gaacttttat
     6481 tttgatggag aggagtggga taaagacgtt tgggatagac gtaacttacc tttgccagat
     6541 gataaggtta acccaacaca atatgaatat acaattaact ttaaagggtt tcggaataca
     6601 tattttaaac aacttgtaaa aagatattgt aagttgagat tgaacatgga tagcttttcc
     6661 tatgtaagtg atattgccca aagacttaaa gagttcttta attttctgga catgaaattt
     6721 aaacaagttc agagagtaca ccaattaacg agagtggaaa ttgaagcata tttaagtgaa
     6781 ctaaacatga tgggaataaa acctagtaca ataactggga ggatctctat attggaagga
     6841 ctatttagta cccttcttag gctagaatgg gatgatgttc cttccaaaat attaatttat
     6901 tctgaggact atccgaaaat accaagagca aaaccacgct ttatagatga attcgtccta
     6961 gagcaattga acagtcatct tgataaatta cccgaatata tagctacgat gactatgatt
     7021 gttcaagaat gtggaatgag gataagtgaa ttgtgcacct tgaaaaaagg ctgtctatta
     7081 gaggacaaag atggagattt ctttttaaag tattatcaat ggaaaatgaa aaaggagcat
     7141 atagttccaa tatctaaaga ggtagcttta cttattaaag ttcgggaaga taaagtttca
     7201 gaggaatttc cagatagtga atacctcttt ccaagaaaag atggatcgcc attaaaacaa
     7261 gaaacattta gaggtgagtt aaataaatta gcttatgagc aaaatatagt ggataaatca
     7321 ggtgagattt atagattcca tgcccatgcc tttcgccata cagtaggaac aagaatgatt
     7381 aacaacggga tgccccagca tattgtgcag aaatttttgg ggcatgaaag cccagaaatg
     7441 acaagcagat acgctcatat ctttgatgaa actctaaaaa atgaatttac taaatttcag
     7501 gaaaaactgg ttaccaataa tggagatgtg cttgatctag atgaagataa tgaagtcgat
     7561 gatgtagagc ttcaatggtt caagaaaaat ataaatgcac aagtgcttcc aaatggttat
     7621 tgtagattgc cagtagtagc aggtggttgt ccacatgcga atgcatgctt agattgcact
     7681 cacttctgta ccagtaagca attcttacca cagcacgaag aacagttaga gcgtacagaa
     7741 gagttattag ccatagctaa ggataaacaa tggcaaagac aagtagagac taatagccgt
     7801 gttaaagagc gtttagaaca aatcattgga agtttgacgg ggtaattatc aatggataaa
     7861 caagttagaa atacaacaga aattgtacgt ttggcgaagc agaaatcaaa aaagacaagg
     7921 gaaaaagtag acaaagcgat ttctaaattt tcgattgaag gtaaagttat taattttaat
     7981 tcaatagcaa aggaagctaa tgtttctaaa tcatggcttt ataaggaaca cgatattagg
     8041 caaagaatcg aatcccttcg tgagcgtcaa ataacagcaa atgtagtctc aaaacccaag
     8101 aaaagttctc gttcggagga aatccttatt aaaaccttaa aaagaagagt aatggaatta
     8161 gaaaaagaaa ataaaaaatt acagaaccaa attcaaaaat tatatggaga tctgtataat
     8221 aaagaataat tattaatctg tagacaaatt gtgaaaggat gtacttaaac gctaacggtc
     8281 agctttattg aacagtcatt taggtatatg tccaatctag ggtaagtaaa ttgagtatca
     8341 atataaactt tatatgaaca taatcagtta cgaggtgaaa tcatgagcaa tttgattaac
     8401 ggaaaaatac caaatcaagc gattcaaaca ttaaaaatcg taaaagattt atttggaagt
     8461 tcaatagttg gagtatatct atttggttca gcagtaaatg gtggtttacg cattaacagc
     8521 gatgtagatg ttctagtcgt cgtgaatcat agtttacctc aattaactcg aaaaaaacta
     8581 acagaaagac taatgactat atcaggaaag attggaaata cggattctgt tagaccactt
     8641 gaagttacgg ttataaatag gagtgaagtt gtcccttggc aatatcctcc aaaaagagaa
     8701 tttatatacg gtgagtggct caggggtgaa tttgagaatg gacaaattca ggaaccaagc
     8761 tatgatcctg atttggctat tgttttagca caagcaagaa agaatagtat ttctctattt
     8821 ggtcctgatt cttcaagtat acttgtctcc gtacctttga cagatattcg aagagcaatt
     8881 aaggattctt tgccagaact aattgagggg ataaaaggtg atgagcgtaa tgtaatttta
     8941 accctagctc gaatgtggca aacagtgact actggtgaaa ttacctcgaa agatgtcgct
     9001 gcagaatggg ctatacctct tttacctaaa gagcatgtaa ctttactgga tatagctaga
     9061 aaaggctatc ggggagagtg tgatgataag tgggaaggac tatattcaaa ggtgaaagca
     9121 ctcgttaagt atatgaaaaa ttctatagaa acttctctca attaggctaa ttttattgca
     9181 ataacaggtg cttactttta aaactactga tttattgata aatattgaac aatttttggg
     9241 aagaataaag cgtcctcttg tgaaattaga gaacgcttta ttactttaat ttagtgaaac
     9301 aatttgtaac tattgaaaat agaaagaaat tgttccttcg atagtttatt aatattagtg
     9361 acatttgcat gcttcaaagc ctgtcggaat tggtttttag tgaaaagaac acgatattca
     9421 cggtttaccc acttataaac aaaagatcga tactttttgt agtccttctt tgaaatcaat
     9481 ggttgatgtc gttcaagaac aatcaataca gagtctacac ttggcttagg atgaaaatat
     9541 agtggtggta cttttttgag catttttata tccatctcca ccattaatag taaacccaaa
     9601 gctcgttgca gattttgcaa tcttttcgca aatcccttct caacgataag atagctatat
     9661 ttagcctgac tttcaaaggt aattcttttg acaatatccg tactgatgtt ataaggaata
     9721 ttaccatata tcttatagtt tatatgtttt gggaaggaaa attttagaat atccgtttga
     9781 atcactttta tattctcaga ggggtttacc gcttctttag tcacttgaca taagcctcca
     9841 tcaatttcta tagcagtaac tgatcgactc attttgacta gctctttggt aaaatgtcct
     9901 tttcctgatc cgatttctat tacgttgtct tgtttactga tattcgtgtg attcaatatt
     9961 tcttttacat gctttttaga agtaataaaa ttttgcgtgt ctttagggtt tttctggttc
    10021 attataacct tctccttact ggttataatg aactgacatt aagcgttcat tataaccaat
    10081 taattttgat ttggttgata acgaacttta ttaataacaa atatagaaaa agtacccata
    10141 cttttatctc ctttccattt ttttcattat gaatgagata aagtaatttc tactactgcg
    10201 atactggtgc acataattga aacctcctta tgtaactgaa tatgattgta tcatcttttc
    10261 attttagaat aaacctttat atgtaatttt gcacttgaca cttcagatgg acgttttctt
    10321 caaaataata tgttttgaaa cttgataaga gaagccactg tctttactaa aaagtaaagg
    10381 ctgcgataaa ggacagtgac tttaataaag ttgcactgtt gtattgtctt tggataaaat
    10441 tgtctaaagg gaagttttat ccccaacgaa aaaacatcta aagtggtaag taatgaaaga
    10501 atgtcgataa agtaggaatg ggggatcttt ttgaatacaa tagaatcaca gtttgataag
    10561 gttgcagaag attacgattt cgtgaatgag cttttgaatg attattcatt ctttgtgtct
    10621 aatatgtctc caaagaaagg cagagcatta gatatcggat gtggctcggg tttgttagtg
    10681 gagaaattag caagttatta tgatgaagtg gtagggattg atatttctaa tcaaatgc
//
LOCUS       DACECJ010000244        10263 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.091, whole genome shotgun sequence.
ACCESSION   DACECJ010000244 DACECJ010000000
VERSION     DACECJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.091"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..465)
                     /locus_tag="IWO09_002943"
     CDS             complement(<1..465)
                     /locus_tag="IWO09_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0265454.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIA"
     gene            complement(807..1679)
                     /locus_tag="IWO09_002944"
     CDS             complement(807..1679)
                     /locus_tag="IWO09_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0265455.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1807..2376
                     /locus_tag="IWO09_002945"
     CDS             1807..2376
                     /locus_tag="IWO09_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0265456.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            2443..3180
                     /locus_tag="IWO09_002946"
     CDS             2443..3180
                     /locus_tag="IWO09_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0265457.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            3411..4679
                     /locus_tag="IWO09_002947"
     CDS             3411..4679
                     /locus_tag="IWO09_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0265458.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            5166..5630
                     /locus_tag="IWO09_002948"
     CDS             5166..5630
                     /locus_tag="IWO09_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0265459.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            5614..6243
                     /locus_tag="IWO09_002949"
     CDS             5614..6243
                     /locus_tag="IWO09_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0265460.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            6597..8075
                     /gene="msr"
                     /locus_tag="IWO09_002950"
     CDS             6597..8075
                     /gene="msr"
                     /locus_tag="IWO09_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0265461.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            8122..8568
                     /locus_tag="IWO09_002951"
     CDS             8122..8568
                     /locus_tag="IWO09_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0265462.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8767..9282)
                     /locus_tag="IWO09_002952"
     CDS             complement(8767..9282)
                     /locus_tag="IWO09_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0265463.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            9399..>10263
                     /locus_tag="IWO09_002953"
     CDS             9399..>10263
                     /locus_tag="IWO09_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0265464.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAF"
BASE COUNT         3280 a         1826 c         2090 g         3067 t
ORIGIN      
        1 tgcgatatat cctgcttcgt cataagtata tggaatccca agctcttttg tcagatactc
       61 tactgcccat tgagcaatac tgaattcatc actgtaaagc acttcgattt ctcttaaaag
      121 tttgttgcgg atgacgatac cgttattaat gttctccgca gaaaaagcaa tgtggtcggt
      181 caaagcgatc aatacatgtt cattcaactt ttccatcaaa acagtttccg catgatcaat
      241 gatcgtttct gctgcaaata aaaagcgttc atcaatctga ttaagtaaat tttgcaactt
      301 gatttgacct tctggttcca tgacaaatat ccgttcgaca tcacgatcaa aaatcaaatc
      361 atttcttttt ctgtcaaatc caatcccttt tccaatagct actttttcct gtccaccgtc
      421 atctaccaat actgcatttt gattaaagat ctttttgatt ttcatgcgcg cacccttttt
      481 gaagatttgg ttttcacgtt tgctagacca gttgattgcg gccaaacaaa aatagacacg
      541 aaaaacctcc tagggaaacc atgttcctca gagatttttc atgcctagta tgatctagtc
      601 acatgaaacg aattattgtt aacgtaatca attttatata gacagttatg ttttgtcaat
      661 tcttttttta taattatagt aatttactgg aattatcaga aataaaagga aggaacaaat
      721 taagaaactt atgttattta atcattggat cttttgctta acttattcgt tgttaaaaag
      781 ctgaaagcga aggaaaaaaa ctctacttaa ctgtatttct ccgttaggac atctaaaaat
      841 ttacgagcag caggagaaag aggctggtct tttttccaaa tgagattcag tgaagcttgt
      901 aatggcggat cgagtgggat gaatactttt tccgtcccat aagtattgat gatcccatct
      961 agagctaatg caccaaaagt cccgttatcg accatcaaag aagcattgta caataagtta
     1021 tatgttccga cgatcgataa ctgatctatg ctgaaaccta gccattcagc tagttgactg
     1081 tccactgacg tttgccggga aatgatcaaa ggaacagttt tcagttcctg cggatggatg
     1141 actgtttgct ttgctaaagg atgattccga tgtatcaaaa gtccccaacg atcttgggct
     1201 ggcagtcgaa atgactggta tttttgtttg tttgtcggtt cgattacgag tccaaaatca
     1261 agtaatccgc tatccagttt gtctatgata tcatctgcat tcccgctata taaatgaaac
     1321 tggatatcag gatgcgcctt gatcaacgaa tgaaggctat cagaaatgaa agagattgct
     1381 ttagtttcgc ctccgccaat gtagatttct ccagagatga ttgcgtcatt tgttaaattg
     1441 ttcgccgttt tttctaccaa cgacaagatt tcttttcctt ttgaataaag atatcgacca
     1501 gaatccgtca actgtatctc tcgggagcct cgagtgaaaa gtgtcacacc aagttcttct
     1561 tctaaatctt tcagttgttt tgagagagta ggttgcgtga tatgcagaga ttttgctgct
     1621 cggctgatat ttttttctct agctactgct aaaaaatacg tcagtatacg aatatccatt
     1681 ttttccctcc tgtttttaaa aaagcatacc actaagtata gttaaaagga atagttaagt
     1741 atgttttata agtattagac atcataagcc gtccgttata aagtaaggat aaaaggaggg
     1801 agcaaaatga caagcctcat cgaaaaacaa atcgtcaata aagagattcg agtaacggat
     1861 ccgctctttg aagtgatcca tcagatccaa gcggaaaacg aagagccttt agcagcgctg
     1921 aatactgggt accatgaacc agcagatatc cgcaaacggc tagaaaatat cattagtgac
     1981 aaagtagatg atacggtgac tgttctgctg cctttttata cagatttcgg caaacatatt
     2041 agtataggga aaaatgtctt tatcaatcgt caagtgatgt tcgttgactt aggtggcatc
     2101 tgtttagaag attcagtatt gattggtcct cgtgtcaacc tcatcacggt gaaccatcta
     2161 accgatccaa aagaacgaag aggcttgtca gtaaagccaa tccacatcaa gaaaaatgct
     2221 tggatcggag caggagcaac tattctgcca ggcgtaacga ttggcgagaa tgccattgtt
     2281 gcagcgaatg caactgttac gaaagatgtc ccagacaata ccatcgttgc aggaatccct
     2341 gccaaaatcg tcaaacctgt cgaacggatt atttgaacag taagatgaaa taagagatag
     2401 tgagtataag caagagattt ttcaaaacag gaggagaata gaatgaatga tttatcagaa
     2461 aaagtgattg tcatcatggg cgcttcaagc ggaatcggtg aagcaacaac gaagctatta
     2521 gcagaaaaag gggctaaact agttatagct gcacgccgag aagatcgcct aaaagcaata
     2581 aaagaatctt taccagaagc tgagctatat attcaaacag cagatgtaag agattttgca
     2641 caagtccaag cagttatcga tttagcgatg gaaaagttcg gtcgaatcga tgtattgtac
     2701 aataatgcag gaatcatgcc aaccgctcca ttagttgaag gtcaccgtga tgaatggcaa
     2761 aatatgcttg atatcaacat catgggtgtg ttaaatggaa tttccgccgt acttccaatc
     2821 atggaaaaac aaaaatctgg gcatatcatt tcaactgatt ctgtggcggg acatgttgtt
     2881 tatcctgatt ccgccgttta ttgcggaaca aaatttgctg tccgtgcaat catggaagga
     2941 ttacgtcaag agcaacggca aaataacatc aaatctacga tcatctctcc aggagctgtt
     3001 caaactgaat tatatcaaac catcagcgac aaaaaggctg ctcttgaact tcatgaagcg
     3061 caaaaagaat ggggattaac atctgaagat atagcttctg ctgtagcatt tgcgatagaa
     3121 acgccagacc ggatgtctgt cagcaacatg attatccgtc cgacgacaca ggaagtataa
     3181 tccataacgg ataatatcca gagagaatta cttgttaaat agttattcaa acgaccatca
     3241 atttaaggcg tgaatggaga agaatattct tcatttacgt ctttttatgc agaaaaagca
     3301 aatttattaa aaagaaacaa atgttattta aatatggaca ttgacttgct tttacttttt
     3361 tagttacact gttatttagg aaaagtttca taattcaaag gagaaaaaag atgaaagcaa
     3421 agaatttatt atttagcgtt attcttttag gaaatctagc agttgccaac acaacagtgc
     3481 tagctgaaga atcgacagaa actgtcgcaa ctcagacatc gagttcttca gaaacaggaa
     3541 ttgaaaacga tacagaaagt actttgcagt cagctactgt ctcagaaaat acaatttcaa
     3601 ccgaagaacc aacaactgaa acaacagaac aaacacaaac cgaagacagt acatcagcag
     3661 aagctcagaa caatccagag aatgcagaag aaatcacgaa ccaattgaca gatgggcaaa
     3721 cattgacaca tacccatcaa gcagatttgt ctgaattcga aaactatact ggctatgctt
     3781 tttatgctcg ctcatctgct aattctcaac aagcgttcat tgatagtatt gcctcaactg
     3841 cacaaagttt ggcaagtgcc aatgacttat atgcatcagt catgattgct caagccatcg
     3901 ttgaaagtgg ctgggggaac agtactttgg cttctgcccc taattacaat ctatttggga
     3961 ttaaaggaag ctataatggc caatctgtta cgatgcctac ttcggaatat gtgaatgggc
     4021 aatggattac ggtgaatgcc gcttttagaa aatatccttc gtacaaagaa tcattgcaag
     4081 ataacgtaac ggtattgaag acgacttctt ttcagccagg cgtttactac tattccggtg
     4141 catggaaaag caatacgaac tcttacaaag atgctacggc ttggctaaca ggtcgttatg
     4201 ccactgcgcc taattatggt tctacattga acaatgtaat cgaaacctat aatctgacac
     4261 agtatgatac agcaccaact tcaacttctc cgatgtatcg tctgtataac cgtcatacag
     4321 gagaacattt atatacattg aatgccggag aaaaaaatta tctgccaact gtaggctggg
     4381 aatatgaagg gattgcttgg caagcaccaa acagcggaca acctgtctac cgtttataca
     4441 atccttacag tggagaccat cattacacaa tggcacaatc tgagatcaac tctttaacaa
     4501 aaatcggttg gcgttatgaa ggtctaagtt tttattctgg tggttccaag ccaatttatc
     4561 gactatttaa tccaaatgaa aaaactggta cccatcacta taccttgagt gctaaggaaa
     4621 gagattggct aacaccgatg ggctggagat atgaaggaat aggattttat ggttattaaa
     4681 aagaggtagt gaaaaagctg ccctgcatca agtagtaaga acagccaaac aaaaacagat
     4741 tttcatgttt tttgtttggt tgaacttatt accgcagatg caagtttttg aacaccgttt
     4801 actcaaaaaa ggttgtgaca acatcttgtc ataacttttt tattactgtt taagcatact
     4861 gatccaattt ctaatattcg acgggattct tgcaaaaggc gatttcggaa atttttggcg
     4921 gtgtaaaatt atctactgca gaagagctat cttctatagt ttgataagaa tcaataatct
     4981 tggttttagc gttgtgcttt ctttatcatt ttttccgttc attctacaaa aaatttcttt
     5041 tctctatact tttctattga gagcgttttt tttattacac agctgattct ttgacttttt
     5101 tcttttttta ttctattatt gacaagtcaa tcgttgacga gtcaattatt agaaaggagg
     5161 aaattatgtc tttagaagag attagtcacc ttttatatca aatcaaatta gccgatcaaa
     5221 aaatcgtcca gatctttgaa gaaaatatcg gtataagtct tactagatac gagatcatga
     5281 tgagactgaa agaagaaaat ccattgctac aaagcagttt gcaagaaaag ttgcagattg
     5341 atcaaggagc agttactcgt catctgaagg tattggaagc aaatggctat gtttcgcgtc
     5401 agagaaatcc tcagaacaac cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga
     5461 aaatcaagca ttgtgaaatg gaccataaag atacaggttc cgtgctatcc ccggctttca
     5521 gcgatgtaga aatgaaacaa tttctaacac ttttagataa attgaatacc caactttgca
     5581 atataacaac agaaacgaaa gaggttaata ataatggaac aaaaaaatga tttttcaaca
     5641 attatgactg gtagaagatc cgttcgcgta tatgatgaaa catacaaaat cccacacgaa
     5701 gaaatgctgg aaatgatcaa agaagcgacg actgcacctt cttctgtcaa tatgaaacca
     5761 tggcgctttg tggtcgctga aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt
     5821 ttcaatacga gacaaaatga tacttcttct gcaatggtca tgatttttgg cgatatgcaa
     5881 tgttacgaat atggcgaaga aatctacaac caagcttacg aatcagggaa gatgccaaaa
     5941 gaagtcagag atcaacaatt agctgcgatc attccttatt acaagagttt ttcaagagaa
     6001 gaaatgaacg atgtagtcaa agtcgattct agcttagcgg ctatgcaatt catgttagtt
     6061 gcgcgtgatc atggctatga aacaaatcca attggtggat tcgaagcaga tcagttggca
     6121 gaggcatttg gtttggataa agaccgttat gttccagtta ttattttatc aataggaaaa
     6181 gcggtagaag aaggttacga gtctgttcgt ttagcaccag aaaagatcac tatgttcgaa
     6241 taaaaggttt tcagttacta ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc
     6301 taataaaaaa ggaggtcgga aaaatgactg catcgatgaa attacgtttc gaacttttga
     6361 ataacaacta aataaattca aaagacatgt ataaacaaca atttcatgga tggagtatgt
     6421 ccattttttc gaacgacagt agaataggtg aaagctatga cagatttggt gatttagtca
     6481 tagttatttc gttcagagta ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc
     6541 tgctcttttt tagtatcgat aattgttgtt ttacatgaaa taggagtgta aaacatatgg
     6601 aaaatttagc agtaaatata acaaatctac aagtcagctt tggcaaccag ctagaactat
     6661 ctattgattc tcttcgtgtc tatcagcaag atcgtatagg gatcatagga gaaaatggtg
     6721 tcggtaaatc gactttgctc aaactaatag ccggtgaact ttttcccgat catgggaaaa
     6781 tccaaacaga gatcaccttc aactacctgc ctcaattaac ctatctcgct gaagcaaagg
     6841 acctaaattt ggaattagcc agtcatttcc agttaagact ggaagaaact tcagagcgga
     6901 aatggagcgg aggggaagaa cgaaagatcg agttgatacg tcttctttct tcttatgaac
     6961 aagggatgct tctagacgag ccaacaaccc atctagatag aaaaagtatt gatcgactga
     7021 ttgaagagct tcgttattat tatggtacgc tggtttttgt tagtcatgat cgctattttc
     7081 tagatgagct agcatcgaaa atctgggaag taaaagacgg agaaatccga gagttttcgg
     7141 ggaattatag tgcctatctc actcaaaagg aattggagaa aaagactcag ctacgagaag
     7201 cagagtcgat catgaaagag aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac
     7261 aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc agactggatc
     7321 ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa
     7381 cattagagag aagactccaa aaaataggag aaacaaccaa gccgcagcag atgaaacaaa
     7441 tccgttttcc agtaccaaaa tctcttgaac tccacagccg ctatcccatc atgggacaaa
     7501 atgtccaatt ggaaagaagt gggagaacat tactggtaaa tggtgatttt cagttttctt
     7561 taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg taagacaacc ttattagaac
     7621 atatccgcaa acaaggagaa ggaatccttc tctctccgaa agtaagcttt caagtatatc
     7681 agcaaaaggg ttatcaaatg acatctgaag aatccatcat tcgttttgtc atgagacaaa
     7741 cagagttttc agaatcgctt gtccgtagtt tgctgaatca cttagggttt gctcaggaaa
     7801 ctttgacgaa accgttatgt acattaagtg ggggagaagc gacccgtctg acgattgctt
     7861 tgctttttac taagccaagt aatgtgttgc tgttagatga accgactaat tttattgata
     7921 tggcaacgat cgaagcttta gagaagctaa tgcaaatata tccgggaacg attttgttta
     7981 cttctcatga ttcttacttt gtcgagcgta cggctgatga agtttatgaa ataaaagggc
     8041 agaaaataaa aaaagtactt acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca
     8101 aatttaaagg agtggtacag attgcctaca aataaaaaag aaggaataat ttttacaaca
     8161 atgatgtgtt ttttgatggt cttggggatg agcgcgtata atttatggct gcatcaagat
     8221 cttcatttat caagtttgtt gattggtcta gtgcctggat tcgtggtggc tttcattttg
     8281 gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc agtaaatcca
     8341 gaaagcaagg tccaaatgat cttaactatt tcatgcttga tggttttagg aatggtgaca
     8401 tgtatgtctt tattcggtgt gatcatggaa caaggaatac ctgatcaatt attctctgct
     8461 tatatgaatg cttggaaaat gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt
     8521 ggcccgcttt caagaagtgt gctaaaagca attcaaagta atgaatgatg gaacactgtt
     8581 atttgaaaaa aggccttggc atgctttttg tcatagtctc ttgaatgcag ttctagaaaa
     8641 atagtgatcc gaacgatagg ttttggcctg tcgttcggat cgctttttct tttccaactg
     8701 tattgctctt ttattgaaga taatttgctc ttcaatagat atttctagaa aggttctgtt
     8761 tatcccttac tcaaaaaact gctgcatttg ttctcttact tgctgacacc attggagttc
     8821 ctgctgacaa agcataattc tgcgctggat cacaacataa cgtccaaatt cttgtttctt
     8881 gtctagcgag aggtcattca attttttttg atttttttgc atgattttgt tcagctgttg
     8941 ttctctagtg aatagcagtc cagtagctgt tgttttatcg agcgtcgaaa aagcatagat
     9001 ttttgctaga aattcatctt tttgcgtagg agcgggagtt tcctctttga tccattcctc
     9061 tacaagagca cgtccttcat cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt
     9121 gtccaaaacg gtcacataac cctgctggtc taacttggca agcgttgtat agatctgact
     9181 gtgatgggct tcccaaaaaa ttgtgatcag ctgcttcaat tcatacccag aacgtggttt
     9241 ggataaaagc acgcataata atatataggc taaagtattc ataaaaatcc tacctctcgt
     9301 cttattttct ttattatatc acagtaaagt ttccacgttt tacatgtgca tattgacatg
     9361 taaaataaat catgtatgat gctaatagag agaaagggat ggaaatattg gagaatcaaa
     9421 tagatataca agaacaaaca aatgaagaaa caaaggggaa aagttcagcg ctagttgtag
     9481 cagctacctg tatgatcgct tttatgggag tgggtctagt agacccaatc ttaaaaacga
     9541 ttgctgcaca gttaaacgct acgccagctg aaacgaccct gttatttact agttacatgt
     9601 tagtcactgg tgtggtgatg ctatttacag gtttcttatc tagtcggatc ggtttgaaaa
     9661 aaacattgat gacaggattg tttatcatcg ttgtattcgc tggtcttggc ggattatctg
     9721 ggaatatcgg aatgctgatt gggttacgtg ccggctgggg tgtcgggaat gctttatttg
     9781 tctctactgc tttagcaacg atcgtcagta ttttagttgg taacacagaa aaagcaatca
     9841 tgatgtatga agcagcactt ggctgcggaa tggctgttgg ccctctagtc ggtggggtct
     9901 tgggcagtgg ttcatggcgt tatccatttt ttggggtagc atttttgatg ttttgcggtt
     9961 ttatagccct agcggtactt ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt
    10021 gggaaggtgt caaagcgtta agcaatcata aacttcgttc agttggacta atcgcattgt
    10081 tatataattt cggattcttt actttacttg cgtatgcgcc atttttattg attggctatt
    10141 ctgaattaga agtcggtttt gtattttttg gctggggtgt gcttctggct attgcttcga
    10201 ttttcgttgc accggctctt gagcggaagt ttacgactta tcggacaatt ttagcagcat
    10261 ttg
//
LOCUS       DACECJ010000245         7677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.101, whole genome shotgun sequence.
ACCESSION   DACECJ010000245 DACECJ010000000
VERSION     DACECJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.101"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..555)
                     /locus_tag="IWO09_002954"
     CDS             complement(<1..555)
                     /locus_tag="IWO09_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0265465.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSI"
     gene            681..1802
                     /locus_tag="IWO09_002955"
     CDS             681..1802
                     /locus_tag="IWO09_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0265466.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2060..3562
                     /locus_tag="IWO09_002956"
     CDS             2060..3562
                     /locus_tag="IWO09_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0265467.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3629..4156
                     /locus_tag="IWO09_002957"
     CDS             3629..4156
                     /locus_tag="IWO09_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0265468.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            4367..5428
                     /locus_tag="IWO09_002958"
     CDS             4367..5428
                     /locus_tag="IWO09_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0265469.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5431..6327
                     /gene="murQ"
                     /locus_tag="IWO09_002959"
     CDS             5431..6327
                     /gene="murQ"
                     /locus_tag="IWO09_002959"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0265470.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            6341..>7677
                     /locus_tag="IWO09_002960"
     CDS             6341..>7677
                     /locus_tag="IWO09_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0265471.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAA"
BASE COUNT         2538 a         1354 c         1719 g         2066 t
ORIGIN      
        1 aatggaataa cgaacgttat ggctatagat cggatttaaa tttgcttctt gaaagacttc
       61 tacaactttt tttgtaaatg cttcgccttc tggttccaag ttctctagtt cgatttctcc
      121 atcacctaac aatgtgactt ttccgggacc agctagacca gctgtccaca tcgtgatccc
      181 aaataagata tttttctttg gcacatattt ttccagtaca tcttcatgac ctaatccatt
      241 tagtaaacac aaaacataag tattgtcagt aataatggat tggatcgaac aaaacatatc
      301 atctaattga ttggcttttg ttaatgctac gatcaagtcc acgtgttcgt tgctttcgat
      361 gatttcttct ggtgaataga ttggcagttt tgcgaccact tctttaccgt taaagtcagc
      421 aatcaaccca tttgtacgaa tggcttcaat gtgagcaggc caacgatcga tcaagaggac
      481 ctcatttccg ctttgatgta gcatcaatcc gattcggctg cccattgctc cggccccagc
      541 tattgcgatt ttcatgtttt ttctctcctt tattcaatga gttccctttt agtatataga
      601 aaaatggaag aaaatagcat tgttttgttt gaaaaaaatg aaaaaaagtg tcttacttaa
      661 gaagaaagtg agggatacag atggatattt tggcggcaat tgattcattt aaaggaagtg
      721 cttcttcatt tgaactaaat caggcagttc taaatggctg ggaagatcgt aaaggaagaa
      781 aaatcaatgt gccgattgca gatggcggag aaaatactgc agaagcaatc tattacgcgc
      841 tcaggggaac atggcgtacc attgatggga tggatctctt attccgtcag cgaaaaatca
      901 gctatttatt gacaacttgg caaggaaaaa acaccgcagt catcgaatct gcacaagcat
      961 tggggttaga tctacttgat aaaccaacgg atcaaactat ccgacttgct tccagttatg
     1021 ccttaggaga acttattaaa gatgcactgc ttcaagaagt agaacaaatt attgtcactt
     1081 taggaggaag cgggtgtagc gatggtggtc taggattact ccaatccttg ggtgcttctt
     1141 tagaagggta cacagaagga aatcctttat tatctactaa aaaacttcat tttgatcgaa
     1201 gaaacgttct tgtttctaaa gtaaaattga ttatcgctgc agatgttacc aatccatata
     1261 caggtcaaca aggagctgct tggcaatttg gaagacaaaa aggaggaata gatacgacgc
     1321 tagctttctt agataaacaa gcaggaaaga ttgctcagca aataaaagat ttctgggagg
     1381 tggatttaga tgatattcca ggaagtggag cagcaggcgg tattggtgga gccctttttt
     1441 tgttgggagc gaagatggtt tctggtttcg atttagtcag ccaaatcact ggattggaaa
     1501 aaaagatcga acaagcagat gtcatcatta caggagaagg gcggatcgat cagcaaacga
     1561 tccacggaaa agttccatac ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa
     1621 tcgctatttg cggaagtaga gaccaagagc tagaaggact ttctgatttt ctcccaattg
     1681 tcttaagtat tcatttgggt ccaataggtt tagaagaagc aatggaacac aggcaaacat
     1741 tagaaaaaac aaaaatctta ggacagacac ttagcagatt tttcaccatc ctcccgaagt
     1801 agttgacaaa atcattttct ttatgctatc gtaatttcaa atataggaca aagtgaggga
     1861 tccgtgcatt gaaaaattaa gtcgattgat gaaagctgca ttcagactgt accttgtgga
     1921 gacaaggaag cagtgtgcag attttcggga ttggcttggt tttttcgatg tatgggtctt
     1981 tttgtgtgta tccaaccctt gcatcctctt tagccagttc tgaaaatagg actggtcttt
     2041 ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa aatctgacat tcggctacga
     2101 cagccaaggc acattattat ttgaacaagc aaatctaaat tttgacacac aatggaaact
     2161 aggacttatc ggacgaaacg gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa
     2221 actaccttat caagggcaag taatccatca gcaagaattt gcctatttcc cgcaacagac
     2281 aaaagataaa gaacgtttaa cctattacgt gttaaatgat attacggatt ttgagatatg
     2341 ggaaatcgaa agagagctcc aattgatgca aacagatcct gaaatcttat ggagagaatt
     2401 cagcacacta tcggggggag agaagacaaa agtcctactg gcacttttat ttgtggatga
     2461 cactcatttc ccgttaatcg atgaaccaac gaatcatttg gatatctctg gtagaaaaca
     2521 agtagcggct tatttgaaaa agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg
     2581 atttatcgat gaagtagtgg accatgtttt agcaatcgaa aaaagtcaac tggaacttta
     2641 tcaagggaat ttctctatct atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc
     2701 tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag aaaacagcag ctgaaaaagc
     2761 cgaatggtct cgttcccgag aaggagataa aacaaagaaa caagtcggat tcatcgatac
     2821 tgaatctaga cgagtgaata aaggagcagt gggtgctgat gctgcacgga cgatgaaacg
     2881 atccaaagca atcgtgaatc ggatggagac ccagatcagc gagaaagaaa aactattaaa
     2941 agatatcgaa tatatcgatt ctttgacgat gaatagccaa gcgtctcacc ataagcgact
     3001 tttaagcgta gaagatcttc aattagggta tgaaaatctg ttattcgagc caattcattt
     3061 tacaatcgag cctcatcagc gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc
     3121 cattatccat tatcttctgg gggcattcaa cggcaaggtt ataggagaaa aataccagcc
     3181 aaaacatctg agcattagtt atgcaagcca aaattatgaa gacaatcgag gaacgttggc
     3241 ggaatttgca gagaaaaacc aagtagacta ccaagcattt ttgaacaacc tccgaaagct
     3301 tgggatggaa agagatgttt ttcataacaa gatcgagcag atgagtatgg gccaacggaa
     3361 aaaagtggaa ttggctaaat ctttatcaca gccagctgaa ctatatacat gggatgaacc
     3421 attgaattat ttggatgtct tcaatcaaga acaattagaa caactgatct tgaacgtgaa
     3481 acctgccatg ttactagtgg aacatgatca aaccttcctg gataaagtat ctactgagat
     3541 tatttctctt gagagaatct aacccttgta atctcaagaa caacaaggta tggttaaggg
     3601 gaactttgcc aacgaaggag agaatcacat gctttatgta acgagacacg gagaaacaac
     3661 ttggaacgca caaggattag tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca
     3721 gatgcaggca cagaaactag cagaaaaggt agtagatttg cctgttccaa tcacgaaaat
     3781 catccattcc cctcttcaac gagcaagaga tacagcacaa gcagttgctg atcgtctcag
     3841 cttgccgctg accgttgatg aacgattggt agaaatggat tttggggatt atgatggact
     3901 gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa
     3961 tggggagtca gtcttagatg tgtatgcacg gatcgttcct ttattgaaag aatgcatcga
     4021 agacgaagaa aatgtctatc tgttagtttg ccataatgcc ttgataagag tgatcaatgc
     4081 ttattttcat ccgatgccta atgaggggtt ctttacattc atggtagata atacagaatt
     4141 ggtttcttat gagtaattat gtttttgtgt tatttaaata aaatatttca aggcactgct
     4201 tttgcttagc agtgcctttt ttaggttatg acaattcttt tgataatata aactcaacct
     4261 ttagaaatga aatttaatta ttttaataaa agtattgaaa ttttgtttct ttagatatat
     4321 actctagtca tagaaatgaa gacggataca gaaaaagagg gataatatgt ttggcttttc
     4381 aatatttatg aacgattctt tgtcagaaga gaagaaacag tatatccgcc aaatggcatc
     4441 caatggcttt gtcggtattt tcacttccat gcatatacca gaagatgatg tttcggcata
     4501 tcaaaagcga ttgatcgaac taggaagcga agcgaaaaag aataaactag aattgatggt
     4561 cgatatttca ggagaggcat tggaacgagc gggtttttca ttagaagatc taacgccttt
     4621 gaaagcaatt ggtgtaacag gtttaagaat ggattatcat atttccaatc agcagattgc
     4681 cgactggtca catcaattga aaatcagttt gaatgctagc acgatcacgc ctaaagatat
     4741 cgatgaattg aaagaagcag aagctgattt ctcacagatt gaagcttggc ataattatta
     4801 tccacgtccc gaaacagggc tggacaaaga atggtatcag aaaaaaaatc aatggctcaa
     4861 aattcaaggt ctccttgtcc aaggttttgt accaggagat acagagttga gaggcccact
     4921 atatcaagga ttgccaactt tggaagaaca tcgagggatc catcctcttg caggcgctct
     4981 agatctatta gcaagtaaca cggatattgt ttacatcgga gatgcaggtc tgtcagaaaa
     5041 cgttcaggag caatttgctt ctttccaaaa agaacagaca gtcttgctcc atacggaacc
     5101 tgtagatgaa gaattttacg aatatatatt agggaagcat acgaatcggc aagacgatgc
     5161 aagagatgtc atacgaagcg tagatgcacg ttttcgagag attccgccta tcccagcaag
     5221 aaatacagct acgcgtatga aagggtctat tactttagat aacgaaaagt atttgcgcta
     5281 tatgggtgag atccaattaa ccaaatatga tttgccagcg gatgaaaaag tgaatgtagt
     5341 agctaaagtt atcaaagaag agctaccatt gatcaaccaa atcaaagctg gtatgaacta
     5401 tcaatttata agaaaagaag gaagataaaa atgagcaaaa tcaatttaga gaacctaaca
     5461 acggaacgca gaaaccaaga aacttttgga ttagatgaga tgagtatttc tcaagccgtt
     5521 caactaatga acaaagaaga tcagaaagtg gccgctgcgg ttgaaaaaga actgacaagt
     5581 atcgaaccag tgattgctaa cacgattgag tcctttaaaa aaggtggacg tctgatttat
     5641 atgggagccg gaacaagtgg aagactaggt gtgttagatg ctgctgaatg cgtaccgaca
     5701 ttcggtgtag aaccggaaat ggtcataggt ctaattgctg gtggagaaga agcaatgact
     5761 gtagctgtcg aaggggccga agattcttat gagctagggc aagaagatct aatcaagctg
     5821 aatttgacaa aaaatgatat agttattggt atcgcagcta gcggtcgtac accatatgtg
     5881 atcggtggat tagattacgc aaaatcgatc ggtgctgtca caggaacgat ttcttgcaat
     5941 aaacaagccg aaatcagtaa acacgcggat ttcccaatcg aagtcgatgc aggtccagaa
     6001 tttttgacag gctctactcg attgaagtca ggaacagcac aaaaacttat cttgaatatg
     6061 atttctacta tcagtatgat tggtataggt aaagtattca ataacttgat ggtcgatgtc
     6121 aaaccaacaa atgaaaaact aatcgaacgc tcaaaacgaa tcatcatgca agcgacagaa
     6181 gctgattacg aaacagctga aaagtacttt gaagaagcag atcaaaatgt taagctggct
     6241 atcgtcatga ttttgacaaa tagtgataaa gaggaagctc aagcaaaatt agctgaagca
     6301 aacggatttg taaaagggac cttatgaggg ggaaaagacc atggcaaaaa acgaactgac
     6361 tttggaagaa ctggcaaaac aaatttataa aggggcaggc ggcatgggca atgtagaaag
     6421 cattgtccac tgcatgactc gtgtgcgtat gaagatcatc aatgatgatc ttgtaaatgt
     6481 ctctgaatta aaagcgattc caggagtgct tggtgtagta gaagatgaac agcttcaagt
     6541 catcatcgga cctgggaaag tgaacaaagt agcacagcaa atggttgatt tggcaggcgt
     6601 gaaattagga gaaaaacttt cttctgtaag tacagctaca gcaaattcat cagcatctgg
     6661 aaaagaccgt gtaaacgaac gggcacaaga aatgaaagcg gcacaaaaat cgaaacaaaa
     6721 accatctaaa atcaaatcgg tattaaaaga catttctaat atctttgtgc caatgattcc
     6781 tgcatttgtc ggtactggga tcgtggcagg gattgccgca gttttagcaa acttagtcac
     6841 agccggaaca cttgatgcag caacatggca acaatatatc gatgtgatga acatcttgaa
     6901 aaacgggatg tttagctacc tagttatcta tacaggtatc aatgctgcac aagtttttgg
     6961 tgcaacacca actcttggtg gagttatcgg ggccgttgtg atgctgacag ggatgaatcc
     7021 ggaagcacca ctgaaaaact tatttactgg tgaagctctt gcagctggac aaggtgggat
     7081 cctaggtgtc atcttcgctg tttggctatt gtcaatcgtt gagaaaaaac tgcataaagt
     7141 agtacctgat gcggtagata tcatcgttac accaactctt tcattgattg ccatcggtct
     7201 gattgaaatt ttcttcatca tgcctttagc aggtttcatt tctgatggca tggtcggtgg
     7261 gatcaactgg gtacttggcg ttggtggagc atttgccggc ttcgttctag gcacattatt
     7321 cttgccgatg gttatgttcg gactgcatca aattttgact ccaatccatg tgcaaatgat
     7381 tgatgaaact ggtcgtacgc tattgcttcc aattcttgcg atggctggtg caggacaagt
     7441 gggtgcagca ttggcattat ggattcgctg caaaaaagat aaagaattaa cagaaatgat
     7501 caaaggagct ttaccagtcg ggatcctagg aatcggcgaa ccattgattt acggagtaac
     7561 attacctcta ggacgcccat ttattactgc ttgtatcggt ggaggtatcg gcggggcagt
     7621 gatcggaatg atcggaaatg tcggggctat cgctattgga ccttcaggag ccgcttt
//
LOCUS       DACECJ010000246         5110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.126, whole genome shotgun sequence.
ACCESSION   DACECJ010000246 DACECJ010000000
VERSION     DACECJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.126"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..687
                     /locus_tag="IWO09_002961"
     CDS             <1..687
                     /locus_tag="IWO09_002961"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0265472.1"
                     /translation="RYIKSPDYNGPDMTDFGHRCLTDPEFNEEYKKQSKLFCEKILTD
                     DSFAETYGDLGHIYGYQWRHWETKDGGFIDQIKQVIEAIKKTPDSRRLIVSAWNPEDV
                     PSMALPPCHTMFQFYVQEGRLSCQLYQRSGDVFLGVPFNIASYALLTHLIAHETGLAV
                     GEFVHTLGDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSVFDFEMEDIQLEGYDP
                     HPAIKAPIAV"
     gene            707..1234
                     /locus_tag="IWO09_002962"
     CDS             707..1234
                     /locus_tag="IWO09_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0265473.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1297..1950)
                     /locus_tag="IWO09_002963"
     CDS             complement(1297..1950)
                     /locus_tag="IWO09_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0265474.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2082..2924
                     /locus_tag="IWO09_002964"
     CDS             2082..2924
                     /locus_tag="IWO09_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0265475.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3082..3978
                     /locus_tag="IWO09_002965"
     CDS             3082..3978
                     /locus_tag="IWO09_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0265476.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            3981..4694
                     /locus_tag="IWO09_002966"
     CDS             3981..4694
                     /locus_tag="IWO09_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0265477.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            4714..>5110
                     /gene="msrA"
                     /locus_tag="IWO09_002967"
     CDS             4714..>5110
                     /gene="msrA"
                     /locus_tag="IWO09_002967"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0265478.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPA"
BASE COUNT         1832 a          927 c         1013 g         1338 t
ORIGIN      
        1 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
       61 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
      121 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
      181 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
      241 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
      301 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
      361 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
      421 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
      481 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
      541 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
      601 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
      661 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
      721 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
      781 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
      841 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
      901 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
      961 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     1021 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     1081 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     1141 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     1201 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     1261 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     1321 gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat
     1381 agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa
     1441 aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca
     1501 ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt
     1561 caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca
     1621 acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag
     1681 aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga
     1741 atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga
     1801 gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc
     1861 gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc
     1921 atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa
     1981 tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac
     2041 atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg
     2101 taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc attcgtgtca
     2161 tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa ctagacggcg
     2221 aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa
     2281 ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa gtgatttcga
     2341 tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc agtaatctaa
     2401 caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta tcttttcagg
     2461 taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag
     2521 tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta gataacttgg
     2581 taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg aacatacgcg
     2641 tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga ggaaagaaaa
     2701 cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca aatgtgcgac
     2761 aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa gagggtttgc
     2821 aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt atcgctacgc
     2881 atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa agaggataag
     2941 ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta ggaaaataat
     3001 tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca ttagaaccaa
     3061 tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct cgttttgttc
     3121 ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc gaaagctggc
     3181 ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga aaaacttcac
     3241 tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa gcaaggagga
     3301 ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc ggttgaaaca
     3361 gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa
     3421 gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt tggcggtaat
     3481 gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc ctttaaaaag
     3541 ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg atccctcaat
     3601 aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa ctttcctgag
     3661 atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa
     3721 aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg attgaatcag
     3781 gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga aaatctaaat
     3841 ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa tttaggttat
     3901 caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca atggttagag
     3961 aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga
     4021 aaacaaatta ctggaaattc gccttcctta tccttcttgg ggtagtactt ggttcgaccg
     4081 tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa ataccagctc
     4141 ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc
     4201 aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa tataaatttt
     4261 atttggaaaa tgaagcacta ctcaatggta ctttcgaagt actaggtttt cctatcgatt
     4321 tctatttata ttttgatccg tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa
     4381 gtctttctat cggaacattg agtttgccaa tcaaagatgt gatgaatatg atcaagagaa
     4441 actataaatt accagattgg atcgaagtca atacagatga tctgactatc atgcttaggt
     4501 tggatcaatt ccgaatgcaa aacgggatgt atattaaagc agataaaatc gacttagtaa
     4561 atgatgacat tcggtttagt ctatatttgc caaaagatac ctctgatgct tcaaacaact
     4621 caaataccaa caaaactagc accagcagta ctgaaagcaa gacaaaagaa aataaaaaat
     4681 catcggatga gtagtattag agggggaatc ataatggaac gagcaatatt tgcaggaggc
     4741 tgcttctggt gtatgatcca gcctttcgac acgcttcctg gtatccatac agtgatgtca
     4801 ggatatactg gagggcatgt accagatccg acttatgaac aagtaaaaag ccaaacgact
     4861 ggacatacag aagcagtgga gattatctat gatccaacat tgatttcata tgaacaatta
     4921 gtagaacttt actggcagca aactgatcca actgacgctt tcggacaatt cgaggatcgc
     4981 ggtgacaatt atcgcccggt gatcttttat ttgacagaag aacagcggat cattgctgaa
     5041 aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc
     5101 gaaccagccc
//
LOCUS       DACECJ010000247         3179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.143, whole genome shotgun sequence.
ACCESSION   DACECJ010000247 DACECJ010000000
VERSION     DACECJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.143"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(40..1026)
                     /locus_tag="IWO09_002968"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="IWO09_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="IWO09_002969"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="IWO09_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0265479.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="IWO09_002970"
     CDS             1851..3005
                     /locus_tag="IWO09_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0265480.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          980 a          626 c          653 g          920 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccg
//
LOCUS       DACECJ010000248         2846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.150, whole genome shotgun sequence.
ACCESSION   DACECJ010000248 DACECJ010000000
VERSION     DACECJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.150"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            81..1049
                     /locus_tag="IWO09_002971"
     CDS             81..1049
                     /locus_tag="IWO09_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ0265481.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1042..2073
                     /gene="vanA"
                     /locus_tag="IWO09_002972"
     CDS             1042..2073
                     /gene="vanA"
                     /locus_tag="IWO09_002972"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ0265482.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2079..2687
                     /locus_tag="IWO09_002973"
     CDS             2079..2687
                     /locus_tag="IWO09_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ0265483.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          844 a          544 c          702 g          756 t
ORIGIN      
        1 ttcagagaaa gatttgacaa gattaccatt ggcatccccg ttttatttgg tgcctttcac
       61 agaaagggtt ggtcttaatt atgaataaca tcggcattac tgtttatgga tgtgagcagg
      121 atgaggcaga tgcattccat gctctttcgc ctcgctttgg cgttatggca acgataatta
      181 acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg
      241 gacataaatc agagatttcc gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat
      301 atatttctac ccgaagcatc ggctgcaatc atatagatac aactgctgct aagagaatgg
      361 gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt tgccgattat actatgatgc
      421 taattcttat ggcagtacgc aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt
      481 tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat gacagttggt gtggtgggaa
      541 cgggccagat aggcaaagcg gttattgagc ggctgcgagg atttggatgt aaagtgttgg
      601 cttatagtcg cagccgaagt atagaggtaa actatgtacc gtttgatgag ttgctgcaaa
      661 atagcgatat cgttacgctt catgtgccgc tcaatacgga tacgcactat attatcagcc
      721 acgaacaaat acagagaatg aagcaaggag catttcttat caatactggg cgcggtccac
      781 ttgtagatac ctatgagttg gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat
      841 tggatgtatt ggaaggagag gaagagtttt tctactctga ttgcacccaa aaaccaattg
      901 ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg
      961 cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg
     1021 attttgaaag gagacaggag catgaataga ataaaagttg caatactgtt tgggggttgc
     1081 tcagaggagc atgacgtatc ggtaaaatct gcaatagaga tagccgctaa cattaataaa
     1141 gaaaaatacg agccgttata cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa
     1201 aaaccttgcg cggaatggga aaacgacaat tgctattcag ctgtactctc gccggataaa
     1261 aaaatgcacg gattacttgt taaaaagaac catgaatatg aaatcaacca tgttgatgta
     1321 gcattttcag ctttgcatgg caagtcaggt gaagatggat ccatacaagg tctgtttgaa
     1381 ttgtccggta tcccttttgt aggctgcgat attcaaagct cagcaatttg tatggacaaa
     1441 tcgttgacat acatcgttgc gaaaaatgct gggatagcta ctcccgcctt ttgggttatt
     1501 aataaagatg ataggccggt ggcagctacg tttacctatc ctgtttttgt taagccggcg
     1561 cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca
     1621 attgaatcgg caagacaata tgacagcaaa atcttaattg agcaggctgt ttcgggctgt
     1681 gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag ttgttggcga ggtggaccaa
     1741 atcaggctgc agtacggaat ctttcgtatt catcaggaag tcgagccgga aaaaggctct
     1801 gaaaacgcag ttataaccgt tcccgcagac ctttcagcag aggagcgagg acggatacag
     1861 gaaacggcaa aaaaaatata taaagcgctc ggctgtagag gtctagcccg tgtggatatg
     1921 tttttacaag ataacggccg cattgtactg aacgaagtca atactctgcc cggtttcacg
     1981 tcatacagtc gttatccccg tatgatggcc gctgcaggta ttgcacttcc cgaactgatt
     2041 gaccgcttga tcgtattagc gttaaagggg tgataagcat ggaaatagga tttacttttt
     2101 tagatgaaat agtacacggt gttcgttggg acgctaaata tgccacttgg gataatttca
     2161 ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt agggacatac gagttggctg
     2221 aatcgctttt gaaggcaaaa gaactggctg ctacccaagg gtacggattg cttctatggg
     2281 acggttaccg tcctaagcgt gctgtaaact gttttatgca atgggctgca cagccggaaa
     2341 ataacctgac aaaggaaagt tattatccca atattgaccg aactgagatg atttcaaaag
     2401 gatacgtggc ttcaaaatca agccatagcc gcggcagtgc cattgatctt acgctttatc
     2461 gattagacac gggtgagctt gtaccaatgg ggagccgatt tgattttatg gatgaacgct
     2521 ctcatcatgc ggcaaatgga atatcatgca atgaagcgca aaatcgcaga cgtttgcgct
     2581 ccatcatgga aaacagtggg tttgaagcat atagcctcga atggtggcac tatgtattaa
     2641 gagacgaacc ataccccaat agctattttg atttccccgt taaataaact tttaaccgtt
     2701 gcacggacaa actatataag ctaactcttt cggcaggaaa cccgacgtat gtaactggtt
     2761 cttagggaat ttatatatag tagatagtat tgaagatgta aggcagaggg ttctgttgca
     2821 aagttttaaa taaagaataa aatccc
//
LOCUS       DACECJ010000249         2100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.168, whole genome shotgun sequence.
ACCESSION   DACECJ010000249 DACECJ010000000
VERSION     DACECJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.168"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..832)
                     /locus_tag="IWO09_002974"
     CDS             complement(<1..832)
                     /locus_tag="IWO09_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0265484.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     G"
     gene            1385..1933
                     /gene="aac(6')"
                     /locus_tag="IWO09_002975"
     CDS             1385..1933
                     /gene="aac(6')"
                     /locus_tag="IWO09_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0265485.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          680 a          372 c          415 g          633 t
ORIGIN      
        1 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
       61 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
      121 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
      181 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
      241 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
      301 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
      361 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
      421 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
      481 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
      541 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
      601 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
      661 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
      721 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
      781 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
      841 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
      901 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
      961 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
     1021 aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct
     1081 aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt
     1141 ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc
     1201 aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta
     1261 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
     1321 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
     1381 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
     1441 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
     1501 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
     1561 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
     1621 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
     1681 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
     1741 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
     1801 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
     1861 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
     1921 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
     1981 aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta
     2041 cttttggaac accgtgtacc agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa
//
LOCUS       DACECJ010000250         1628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.180, whole genome shotgun sequence.
ACCESSION   DACECJ010000250 DACECJ010000000
VERSION     DACECJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.180"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..859)
                     /locus_tag="IWO09_002976"
     CDS             complement(<1..859)
                     /locus_tag="IWO09_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0265486.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHV
                     EIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLG
                     YLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLE
                     QAFRKAGTEL"
     gene            1089..1586
                     /gene="dfrG"
                     /locus_tag="IWO09_002977"
     CDS             1089..1586
                     /gene="dfrG"
                     /locus_tag="IWO09_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0265487.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          566 a          253 c          288 g          521 t
ORIGIN      
        1 acaattctgt tccagctttt ctaaaggctt gctccagtac aataggagta agcatccgaa
       61 aatctgctgg agtactaaca gggctatagt ataaaccgta cttaaaacag attttacaat
      121 ccgtcacatt ccaaccatat aatccttgtt cgcaaccata gcgtatccct tccataactg
      181 cattttgaaa tgattgattt aagtatccaa gagaaaccga gctctcatac tgcattccac
      241 ttcccaacgg aagcggtgat acagataaac caatggaagc ccagaaagga tttggcggca
      301 cttcgatgtg aatggtatat tctgcatttt ttaacggtct ctccatataa atgactgtag
      361 gctcttttag ttctatctcc acatgatact tttcttgcaa cagtgcacta atcacttcca
      421 tttgtacttt ccctaagaaa gaaagtataa tttcatgtgt cgtagaatcc acgtaatatc
      481 gtagaagcgg atcactatct gagatttcca aaagggcatc aagcaacatt tctctctgtt
      541 caggtttact cggttcaaca gttgtttgta gtagagggtg cggattttca atcttttttc
      601 tctgtggcaa tagttttgta tctccaagaa cactatttaa cttcaaaaac tcattttgca
      661 aaataacaat ttctccagaa taagctctat caatcttaca taattcacca tttattgaag
      721 tatacatttc tgtaactttt attttttctt tttctgatac tctaaccgaa tctcgtaaat
      781 gtagtactcc actataaagg cgtatatatg caagtaagta gttcagtttt ggagtacaaa
      841 atgacaaaat gtacttcaaa atggactact tttttcattt taacttacat accaaattac
      901 atacaaaatg atttataaaa tgcaattgaa aatgataatt gaatgaaaat ttgtgatttt
      961 gtggtattat gataatataa cttatagaaa aaattgctta ttcaactaac gggcaggata
     1021 atgagaaaag gttttttgat agaaataaga aattagatgg taaattagtt aaaaaggctg
     1081 tgttataaat gaaagtttct ttgattgctg cgatggataa gaatagagtg attggcaaag
     1141 agaatgacat tccttggagg attcccaagg actgggaata tgttaaaaat actacaaagg
     1201 gacatccgat aatattaggt aggaagaacc ttgaatcaat cggaagagcc ttacctgaca
     1261 gaagaaatat tattctgacg agagataagg ggtttacctt taatggttgt gaaattgttc
     1321 attcaataga agatgttttt gagttatgta aaaacgaaga agaaattttt attttcggag
     1381 gagaacagat ttataatttg tttttccctt atgttgagaa aatgtacatc acaaaaatac
     1441 atcatgaatt cgaaggagat actttttttc cagaagtgaa ttatgaggaa tggaatgagg
     1501 tatttgccca aaaagggata aagaatgata aaaatccgta taactactat tttcatgtat
     1561 atgaaagaaa aaacttattg agttaacggg tgcgttaatt gaagaagaac agtaaagata
     1621 aagcacaa
//
LOCUS       DACECJ010000251         1562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.181, whole genome shotgun sequence.
ACCESSION   DACECJ010000251 DACECJ010000000
VERSION     DACECJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.181"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..155)
                     /locus_tag="IWO09_002978"
     CDS             complement(<1..155)
                     /locus_tag="IWO09_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0265488.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIG"
     gene            517..1557
                     /locus_tag="IWO09_002979"
     CDS             517..1557
                     /locus_tag="IWO09_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0265489.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT          509 a          263 c          272 g          518 t
ORIGIN      
        1 ccgattgatt caaggttctt cctacctaat attatcggat gtccctttgt agtattttta
       61 acatattccc agtccttggg aatcctccaa ggaatgtcat tctctttgcc aatcactcta
      121 ttcttatcca tcgcagcaat caaagaaact ttcatttata acacagcctt tttaactaat
      181 ttaccatcta atttcttatt tctatcaaaa aaccttttct cattatcctg cccgttagtt
      241 gaataagcaa ttttttctat aagttatatt atcataatac cacaaaatca caaattttca
      301 ttcaattatc attttcaatt gcattttata aatcattttg tatgtaattt ggtatgtaag
      361 ttaaaatgaa aaaagtagtc cattttgaag tacattttgt cattttgtac tccaaaactg
      421 aactacttac ttgcatatat acgcctttat agtggagtac tacatttacg agattcggtt
      481 agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat aaatggtgaa
      541 ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa tgagtttttg
      601 aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat
      661 ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg
      721 cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta cgtggattct
      781 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
      841 ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt catttatatg
      901 gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc aaatcctttc
      961 tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag
     1021 agctcggttt ctcttggata cttaaatcaa tcatttcaaa atgcagttat ggaagggata
     1081 cgctatggtt gcgaacaagg attatatggt tggaatgtga cggattgtaa aatctgtttt
     1141 aagtacggtt tatactatag ccctgttagt actccagcag attttcggat gcttactcct
     1201 attgtactgg agcaagcctt tagaaaagct ggaacagaat tgttagagcc atatcttagt
     1261 tttaaagttt atgcaccaca ggaatatctt tcacgggcat ataacgatgc tcccaaatat
     1321 tgtgcaaata tcgtaaatac tcaactgaaa aataatgagg tcattattat tggagaaatt
     1381 cctgctcgat gtattcaaga ttatcgcaat gatttaactt tttttacaaa tgggcttagt
     1441 gtttgtttag cagagctaaa aggatatcag gttaccactg gcgaacctgt ttgccagacc
     1501 cgtcgtctaa atagtcggat agataaagta agatatatgt tcaataaaat aacttagtgc
     1561 gt
//
LOCUS       DACECJ010000252         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.184, whole genome shotgun sequence.
ACCESSION   DACECJ010000252 DACECJ010000000
VERSION     DACECJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.184"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWO09_002980"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWO09_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWO09_002981"
     CDS             394..477
                     /locus_tag="IWO09_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0265490.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO09_002982"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWO09_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0265491.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACECJ010000253         1421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.189, whole genome shotgun sequence.
ACCESSION   DACECJ010000253 DACECJ010000000
VERSION     DACECJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.189"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IWO09_002983"
     CDS             <1..1350
                     /locus_tag="IWO09_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ0265492.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          597 a          114 c          239 g          471 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
     1381 gacggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
LOCUS       DACECJ010000254         1350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.191, whole genome shotgun sequence.
ACCESSION   DACECJ010000254 DACECJ010000000
VERSION     DACECJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.191"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..298
                     /locus_tag="IWO09_002984"
                     /pseudo
     CDS             47..298
                     /locus_tag="IWO09_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="IWO09_002985"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="IWO09_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ0265493.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DACECJ010000255         1197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41211_2017
            SAMN12640045-rid9880053.guided.201, whole genome shotgun sequence.
ACCESSION   DACECJ010000255 DACECJ010000000
VERSION     DACECJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640045
            Sequence Read Archive: SRR10023244
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741769.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 01:36:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,934
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 34 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 7 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640045-rid9880053.guided.201"
                     /isolate="E41211_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            260..>1197
                     /gene="vanY"
                     /locus_tag="IWO09_002986"
     CDS             260..>1197
                     /gene="vanY"
                     /locus_tag="IWO09_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ0265494.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          428 a          174 c          238 g          357 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt ggttctgttg caaagtttta aatctactat caaataa
//
